SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011915091.1 NCBI__GCF_000013785.1:WP_011915091.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9X0C7 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
33% identity, 94% coverage: 1:233/247 of query aligns to 1:233/241 of Q9X0C7

query
sites
Q9X0C7

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D8 (= D8) mutation to N: Loss of activity.
H48 (= H48) mutation to A: Decrease in activity.
D51 (= D51) mutation to N: Decrease in activity.
R83 (= R85) mutation to N: Decrease in activity.
D127 (= D130) mutation to N: Almost no activity.
T164 (= T167) mutation to A: Strong decrease in activity.

4tx9A Crystal structure of hisap from streptomyces sviceus with degraded profar (see paper)
32% identity, 95% coverage: 3:236/247 of query aligns to 11:240/246 of 4tx9A

query
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4tx9A

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active site: D16 (= D8), D135 (= D130)
binding aminoimidazole 4-carboxamide ribonucleotide: G28 (= G20), V57 (= V50), L59 (= L52), S86 (≠ G81), G88 (= G83), R90 (= R85), G109 (= G104), T110 (= T105), D135 (= D130), G145 (= G140), W146 (= W141)

5dn1A Crystal structure of phosphoribosyl isomerase a from streptomyces coelicolor (see paper)
33% identity, 95% coverage: 3:236/247 of query aligns to 6:235/240 of 5dn1A

query
sites
5dn1A

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active site: D11 (= D8), D130 (= D130)
binding aminoimidazole 4-carboxamide ribonucleotide: G23 (= G20), L54 (= L52), F58 (= F56), S81 (≠ G81), G83 (= G83), R85 (= R85), G104 (= G104), T105 (= T105), G140 (= G140), W141 (= W141)

P16250 Phosphoribosyl isomerase A; 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase; N-(5'-phosphoribosyl)anthranilate isomerase; PRAI; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; EC 5.3.1.16; EC 5.3.1.24 from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) (see paper)
33% identity, 95% coverage: 3:236/247 of query aligns to 6:235/240 of P16250

query
sites
P16250

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D11 (= D8) mutation to A: No activity.
R19 (= R16) mutation to A: No effect on activity toward PRA. No activity toward ProFAR.
S81 (≠ G81) mutation to T: No activity toward PRA. Almost no effect on activity toward ProFAR.
D130 (= D130) mutation to A: Very low activity toward PRA. No activity toward ProFAR.; mutation to Q: No activity.
T166 (= T167) mutation to A: No activity.
D171 (= D172) mutation to A: Low activity toward PRA. No activity toward ProFAR.

3zs4A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound prfar
32% identity, 96% coverage: 1:238/247 of query aligns to 3:240/244 of 3zs4A

query
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3zs4A

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P

E

Q

A

A

Q

L

A

A

active site: D10 (= D8), D129 (= D130)
binding phosphoric acid mono-[5-({[5-carbamoyl-3-(5-phosphonooxy-5-deoxy-ribofuranosyl)- 3h-imidazol-4-ylamino]-methyl}-amino)-2,3,4-trihydroxy-pentyl] ester: A8 (= A6), D10 (= D8), R18 (= R16), A56 (= A55), F57 (= F56), S80 (≠ G81), G82 (= G83), R84 (= R85), G103 (= G104), T104 (= T105), D129 (= D130), G143 (= G140), W144 (= W141), D174 (= D172), G175 (= G173), G200 (= G198), G201 (= G199), I223 (= I221), G225 (= G223), K226 (≠ R224)

2y88A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase (variant d11n) with bound prfar (see paper)
31% identity, 96% coverage: 1:238/247 of query aligns to 3:240/244 of 2y88A

query
sites
2y88A

M

L

L

I

I

L

P

P

A
|
A

I

V

D
x
N

L

V

K

V

D

E

G

G

A

R

C

A

V

V

R
|
R

L

L

R

V

Q

Q

G
|
G

L

K

M

A

D

G

D

S

A

Q

T

T

V

E

F

Y

S

G

D

S

D

-

P

A

V

V

A

D

M

A

A

A

A

L

K

G

W

W

V

Q

Q

R

A

D

G

G

C

A

R

E

R

W

L

I

H

H

L

L

V

V

D

D

L
|
L

N

D

G

A

A
|
A

F

F

E

-

G

G

Q

R

P

G

V

S

N

N

G

H

E

E

V

L

T

A

A

E

I

V

A

V

K

G

R

K

Y

L

P

-

D

D

L

V

P

Q

I

V

Q

E

I

L

G
x
S

G
|
G

I

I

R
|
R

T

D

L

D

E

E

T

S

I

L

E

A

H

A

Y

A

V

L

R

A

A

T

G

G

V

C

S

A

Y

R

V

V

I

N

I

V

G
|
G

T
|
T

K

A

A

A

V

L

K

E

E

N

P

P

E

Q

F

W

V

C

T

A

E

R

A

V

C

I

R

G

A

E

F

H

P

G

G

D

K

Q

V

V

I

A

V

V

G

G

L

L

D
|
D

A

V

K

Q

-

I

D

D

G

G

-

E

-

H

F

R

V

L

A

R

T

G

D

R

G
|
G

W
|
W

A

-

E

E

V

T

S

D

S

G

V

G

Q

D

A

L

V

W

D

D

L

V

A

L

R

E

R

R

F

L

E

D

A

S

D

E

G

G

V

C

S

S

A

R

I

F

V

V

Y

V

T

T

D

D

I

I

A

T

K

K

D
|
D

G
|
G

M

T

M

L

Q

G

G

G

C

P

N

N

V

L

E

D

A

L

T

L

V

A

A

G

L

V

A

A

N

D

A

R

S

T

R

D

I

A

P

P

V

V

I

I

A

A

S
|
S

G
|
G

G

G

I

V

H

S

N

S

I

L

G

D

D

D

I

L

Q

R

K

A

L

I

L

A

D

T

T

L

N

T

T

H

P

R

G

G

I

V

I

E

G

G

A

A

I

I

T

V

G
|
G

R
x
K

A

A

I

L

Y

Y

E

A

G

R

T

R

L

F

D

T

V

L

A

P

E

Q

A

A

Q

L

A

A

active site: N10 (≠ D8), D129 (= D130)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: A8 (= A6), N10 (≠ D8), R18 (= R16), G22 (= G20), L53 (= L52), A56 (= A55), S80 (≠ G81), G81 (= G82), G82 (= G83), R84 (= R85), G103 (= G104), T104 (= T105), D129 (= D130), G143 (= G140), W144 (= W141), D174 (= D172), G175 (= G173), S199 (= S197), G200 (= G198), G225 (= G223), K226 (≠ R224)

5abtA S.Enterica hisa mutant d7n, g102a, v106m, d176a
35% identity, 95% coverage: 2:236/247 of query aligns to 1:238/246 of 5abtA

query
sites
5abtA

L

M

I

I

P

P

A

A

I

L

D
x
N

L

L

K

I

D

D

G

G

A

T

C

V

V

V

R

R

L

L

R

H

Q

Q

G
|
G

L

D

M

Y

D

A

D

R

A

Q

T

R

V

D

F

Y

S

G

D

N

D

D

P

P

V

L

A

P

M

R

A

L

A

Q

K

D

W

Y

V

A

Q

A

A

Q

G

G

C

A

R

G

R

V

L

L

H

H

L

L

V
|
V

D

D

L
|
L

N

T

G

G

A

A

F

K

E

D

G

P

Q

A

-

K

-

R

-

Q

-

I

P

P

V

L

N

I

G

K

E

T

V

L

V

V

T

A

A

G

I

V

A

-

K

-

R

-

Y

-

P

-

D

N

L

V

P

P

I

V

Q

Q

I

V

G

G

G
|
G

I

V

R
|
R

T

T

L

E

E

E

T

D

I

V

E

A

H

A

Y

L

V

L

R

K

A

A

G

G

V

V

S

A

Y

R

V

V

I
x
V

I

I

G
x
A

T
x
S

K

T

A

A

V

M

K

K

E

S

P

P

E

D

F

V

V

V

T

K

E

G

A

W

C

F

R

E

A

R

F

F

P

G

G

A

K

Q

V

A

I

L

V

V

-

L

G

A

L

L

D
|
D

A

V

K

R

-

I

-

D

-

E

-

H

-

G

D

T

G

K

F

Q

V

V

A

A

T

V

D

S

G
|
G

W
|
W

A

Q

E

E

V

N

S

S

S

G

V

V

Q

S

A

L

V

E

D

Q

L

L

A

V

R

E

R

T

F

Y

E

L

A

P

D

V

G

G

V

L

S

K

A

H

I

V

V

L

Y

C

T

T

D

D

I

I

A

S

K

R

D

A

G
|
G

M

T

M

L

Q

A

G

G

C

S

N

N

V

V

E

S

A

L

T

Y

V

E

A

E

L

V

A

-

N

-

A

C

S

A

R

R

I

Y

P

P

V

Q

I

I

A

A

-

F

-

Q

-

S

S
|
S

G
|
G

G

G

I

I

H

G

N

D

I

I

G

D

D

D

I

I

Q

A

K

A

L

L

L

R

D

G

T

T

N

-

T

-

P

-

G

G

I

V

I

R

G

G

A

V

I
|
I

T

V

G
|
G

R
|
R

A

A

I

L

Y

L

E

E

G

G

T

K

L

F

D

T

V

V

A

K

E

E

A

A

active site: N7 (≠ D8), D129 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D8), G19 (= G20), V49 (= V50), L51 (= L52), G81 (= G83), R83 (= R85), V100 (≠ I102), A102 (≠ G104), S103 (≠ T105), D129 (= D130), G144 (= G140), W145 (= W141), G177 (= G173), S202 (= S197), G203 (= G198), I223 (= I221), G225 (= G223), R226 (= R224)

2y85A Crystal structure of mycobacterium tuberculosis phosphoribosyl isomerase with bound rcdrp (see paper)
31% identity, 96% coverage: 1:238/247 of query aligns to 3:231/234 of 2y85A

query
sites
2y85A

M

L

L

I

I

L

P

P

A

A

I

V

D
|
D

L

V

K

V

D

E

G

G

A

R

C

A

V

V

R

R

L

T

R

E

Q

Y

G

G

L

S

M

A

D

V

D

D

A

A

T

-

V

-

F

-

S

-

D

-

P

-

V

-

A

-

M

-

A

A

A

L

K

G

W

W

V

Q

Q

R

A

D

G

G

C

A

R

E

R

W

L

I

H
|
H

L

L

V
|
V

D

D

L
|
L

N

D

G

A

A
|
A

F

F

E

-

G

G

Q

R

P

G

V

S

N

N

G

H

E

E

V

L

T

A

A

E

I

V

A

V

K

G

R

K

Y

L

P

-

D

D

L

V

P

Q

I

V

Q

E

I

L

G
x
S

G

G

I

I

R

R

T

D

L

D

E

E

T

S

I

L

E

A

H

A

Y

A

V

L

R

A

A

T

G

G

V

C

S

A

Y

R

V

V

I

N

I

V

G

G

T

T

K

A

A

A

V

L

K

E

E

N

P

P

E

Q

F

W

V

C

T

A

E

R

A

V

C

I

R

G

A

E

F

H

P

G

G

D

K

Q

V

V

I

A

V

V

G

G

L

L

D
|
D

A

V

K

Q

-

I

D

D

G

G

-

E

-

H

F

R

V

L

A

R

T

G

D
x
R

G

G

W

W

A

-

E

E

V

T

S

D

S

G

V

G

Q

D

A

L

V

W

D

D

L

V

A

L

R

E

R

R

F

L

E

D

A

S

D

E

G

G

V

C

S

S

A

R

I

F

V

V

Y

V

T

T

D

D

I

I

A

T

K

K

D
|
D

G
|
G

M

T

M

L

Q

G

G

G

C

P

N

N

V

L

E

D

A

L

T

L

V

A

A

G

L

V

A

A

N

D

A

R

S

T

R

D

I

A

P

P

V

V

I

I

A

A

S
|
S

G
|
G

I

V

H

S

N

S

I

L

G

D

D

D

I

L

Q

R

K

A

L

I

L

A

D

T

T

L

N

T

T

H

P

R

G

G

I

V

I

E

G

G

A

A

I

I

T

V

G
|
G

R
x
K

A

A

I

L

Y

Y

E

A

G

R

T

R

L

F

D

T

V

L

A

P

E

Q

A

A

Q

L

A

A

active site: D10 (= D8), D120 (= D130)
binding 1-(o-carboxy-phenylamino)-1-deoxy-d-ribulose-5-phosphate: D10 (= D8), H40 (= H48), V42 (= V50), L44 (= L52), A47 (= A55), S71 (≠ G81), R133 (≠ D139), D165 (= D172), G166 (= G173), S190 (= S197), G191 (= G198), G192 (= G199), G216 (= G223), K217 (≠ R224)

5ab3A S.Enterica hisa mutant d7n, d10g, dup13-15, q24l, g102a (see paper)
35% identity, 95% coverage: 2:236/247 of query aligns to 1:235/241 of 5ab3A

query
sites
5ab3A

L

M

I

I

P

P

A

A

I

L

D
x
N

L

L

K

I

D

G

G

G

A

T

C

V

V

V

R

R

-

V

-
x
R

L

L

R

H

Q

Q

G

G

L

D

M
x
Y

D

A

D

R

A

L

T

R

V

D

F

Y

S

G

D

N

D

D

P

P

V

L

A

P

M

R

A

L

A

Q

K

D

W

Y

V

A

Q

A

A

Q

G

G

C

A

R

G

R

V

L

L

H

H

L

L

V
|
V

D

D

L

L

N

T

G

G

A

A

F

K

E

D

G

P

Q

A

-

K

-

R

-

Q

-

I

P

P

V

L

N

I

G

K

E

T

V

L

V

V

T

A

A

G

I

V

A

-

K

-

R

-

Y

-

P

-

D

N

L

V

P

P

I

V

Q

Q

I

V

G

G

G
|
G

I

V

R

R

T

T

L

E

E

E

T

D

I

V

E

A

H

A

Y

L

V

L

R

K

A

A

G

G

V

V

S

A

Y

R

V

V

I

V

I

I

G
x
A

T
x
S

K

T

A

A

V

V

K

K

E

S

P

P

E

D

F

V

V

V

T

K

E

G

A

W

C

F

R

E

A

R

F

F

P

G

G

A

K

Q

V

A

I

L

V

V

-

L

G
x
A

L

L

D
|
D

A

V

K

R

D

I

G

D

F

E

V

H

A

G

T

T

D

K

G

Q

W

V

A

A

E

-

V

V

S

S

S

G

V

V

Q

S

A

L

V

E

D

Q

L

L

A

V

R

E

R

T

F

Y

E

L

A

P

D

V

G

G

V

L

S

K

A

H

I

V

V

L

Y

C

T

T

D

D

I

I

A

S

K

R

D

D

G
|
G

M

T

M

L

Q

A

G

G

C

S

N

N

V

V

E

S

A

L

T

Y

V

E

A

E

L

V

A

-

N

-

A

C

S

A

R

R

I

Y

P

P

V

Q

I

I

A

A

-

F

-

Q

-

S

S
|
S

G
|
G

I

I

H

G

N

D

I

I

G

D

D

D

I

I

Q

A

K

A

L

L

L

R

D

G

T

T

N

-

T

-

P

-

G

G

I

V

I

R

G

G

A

V

I

I

T

V

G
|
G

R
|
R

A

A

I

L

Y

L

E

E

G

G

T

K

L

F

D

T

V

V

A

K

E

E

A

A

active site: N7 (≠ D8), D132 (= D130)
binding [(2r,3s,4r,5r)-5-[4-aminocarbonyl-5-[[(z)-[(3r,4r)-3,4-dihydroxy-2-oxo-5-phosphonooxy-pentyl]iminomethyl]amino]imidazol-1-yl]-3,4-dihydroxy-oxolan-2-yl]methyl dihydrogen phosphate: N7 (≠ D8), R18 (vs. gap), Y24 (≠ M22), V52 (= V50), G84 (= G83), A105 (≠ G104), S106 (≠ T105), A130 (≠ G128), D132 (= D130), G174 (= G173), S199 (= S197), G200 (= G198), G201 (= G199), G222 (= G223), R223 (= R224)

1h5yB Hisf protein from pyrobaculum aerophilum (see paper)
34% identity, 81% coverage: 1:199/247 of query aligns to 5:206/253 of 1h5yB

query
sites
1h5yB

M

L

L

R

I

I

P

P

A

C

I

L

D
|
D

L

I

K

D

D

G

G

G

A

A

C

K

V

V

R

V

L

V

R
x
K

-

G

-

V

-

N

-

F

Q

Q

G

G

L

I

M

R

D

E

D

-

A

-

T

-

V

-

F

-

S

V

D

G

D

D

P

P

V

V

A

E

M

M

A

A

A

V

K

R

W

Y

V

E

Q

E

A

E

G

G

C

A

R

D

R

E

L

I

H

A

L

I

V

L

D

D

L

I

N

T

G

A

A

A

F

P

E

E

G

G

Q

R

P

A

V

T

N

F

G

I

E

D

V

S

V

V

T

K

A

R

I

V

A

A

K

E

R

A

Y

V

P

-

D

S

L

I

P

P

I

V

Q

L

I

V

G

G

G
|
G

I

V

R

R

T

S

L

L

E

E

T

D

I

A

E

T

H

T

Y

L

V

F

R

R

A

A

G

G

V

A

S

D

Y

K

V

V

I

S

I

V

G
x
N

T
|
T

K

A

A

A

V

V

K

R

E

N

P

P

E

Q

F

L

V

V

T

A

E

L

A

L

C

A

R

R

A

E

F

F

P

G

G

S

K

Q

-

S

V

T

I

V

V

V

G
x
A

L

I

D
|
D

A

A

K

K

-

W

D

N

G

G

F

E

-

Y

-

E

V

V

A

Y

T

V

D

K

G
|
G

W

G

A

R

E

E

V

A

S

T

S

G

V

L

Q

D

A

A

V

V

D

K

L

W

A

A

R

K

R

E

F

V

E

E

A

E

D

L

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T
|
T

D

S

I

I

A

D

K

R

D
|
D

G
|
G

M

T

M

G

Q

L

G

G

C

Y

N

D

V

V

E

E

A

L

T

I

V

R

A

R

L

V

A

A

N

D

A

S

S

V

R

R

I

I

P

P

V

V

I

I

A

A

S

S

G

G

active site: D12 (= D8), D133 (= D130)
binding glycerol: K22 (≠ R18), N106 (≠ G104), A131 (≠ G128), D133 (= D130), G147 (= G140), T174 (= T167), D179 (= D172)
binding phosphate ion: G84 (= G82), G85 (= G83), N106 (≠ G104), T107 (= T105), D179 (= D172), G180 (= G173)

Sites not aligning to the query:

binding phosphate ion: 226, 227, 228

Q9X0C6 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
30% identity, 80% coverage: 3:199/247 of query aligns to 6:203/253 of Q9X0C6

query
sites
Q9X0C6

I

I

P

A

A
x
C

I

L

D
|
D

L

V

K

K

D

D

G

G

A

R

C

V

V

V

R
x
K

L

-

R

-

Q

-

G

G

L

T

M

N

D

F

D

E

A

N

T

L

V

R

F

D

S

S

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

V

L

F

V

L

D
|
D

L

I

N

T

G

A

A

S

F

V

E

E

G

K

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

S

I

I

G
x
N

T

T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G

A

L

I

D
|
D

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T

T

D

S

I

I

A

D

K

R

D
|
D

G

G

M

T

M

K

Q

S

G

G

C

Y

N
x
D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

C9 (≠ A6) mutation to A: No change in activity.
D11 (= D8) mutation to X: Loss of activity.
K19 (≠ R16) mutation to S: Decrease in activity.
D51 (= D51) mutation to N: No change in activity.
N103 (≠ G104) mutation to A: No change in activity.
D130 (= D130) mutation D->A,C,F,G,H,I,K,L,M,N,P,Q,R,S,T,V,W,Y: Complete loss of activity.; mutation to E: Weak activity.
D176 (= D172) mutation to N: Decrease in activity.
D183 (≠ N179) mutation to N: No change in activity.

7ac8A Molecular basis for the unique allosteric activation mechanism of the heterodimeric imidazole glycerol phosphate synthase complex. (see paper)
30% identity, 80% coverage: 3:199/247 of query aligns to 6:203/252 of 7ac8A

query
sites
7ac8A

I

I

P

A

A

C

I

L

D
|
D

L

V

K

K

D

D

G

G

A

R

C

V

V

V

R

K

L

-

R

-

Q

-

G

G

L

T

M

N

D

F

D

E

A

N

T

L

V

R

F

D

S

S

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

V

L

F

V
x
L

D

D

L
x
I

N

T

G

A

A

S

F

V

E

E

G

K

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

G
|
G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

S

I

I

G
x
N

T
|
T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G

A

L

I

D
|
D

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G
x
S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T

T

D

S

I

I

A

D

K

R

D
|
D

G
|
G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S
|
S

G

G

active site: D11 (= D8), D130 (= D130)
binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: L50 (≠ V50), I52 (≠ L52), G82 (= G83), N103 (≠ G104), T104 (= T105), D130 (= D130), S144 (≠ G140), D176 (= D172), G177 (= G173), S201 (= S197)

Sites not aligning to the query:

binding [(2R,3S,4R,5R)-5-[4-aminocarbonyl-5-[(E)-[[(2R,3R,4S,5R)-3,4-bis(oxidanyl)-5-(phosphonooxymethyl)oxolan-2-yl]amino]methylideneamino]imidazol-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methyl dihydrogen phosphate: 225

3zr4E Structural evidence for ammonia tunneling across the (beta-alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex (see paper)
30% identity, 80% coverage: 3:199/247 of query aligns to 6:194/244 of 3zr4E

query
sites
3zr4E

I

I

P

A

A

C

I

L

D
|
D

L

V

K

K

D

D

G

G

A

R

C

V

V

V

R

K

L

-

R

-

Q

-

G

-

L

-

M

-

D

-

A

-

T

-

V

-

F

-

S

S

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

V

L

F

V

L

D

D

L

I

N

T

G

A

A

S

F

V

E

E

G

K

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

S

I

I

G

N

T

T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G

A

L

I

D
|
D

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T

T

D

S

I

I

A

D

K

R

D

D

G

G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V
x
R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

active site: D11 (= D8), D121 (= D130)
binding glycerol: R179 (≠ V184)

Sites not aligning to the query:

binding glycerol: 206

1gpwC Structural evidence for ammonia tunneling across the (beta/alpha)8 barrel of the imidazole glycerol phosphate synthase bienzyme complex. (see paper)
29% identity, 76% coverage: 13:199/247 of query aligns to 8:203/253 of 1gpwC

query
sites
1gpwC

A

A

C

C

V

L

R
x
N

L

V

R

K

Q

D

G

G

L

R

M

V

D

V

D

K

A

G

T

T

V

N

F

F

-

E

-

N

-

L

-

R

-

D

S

S

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

V

L

F

V

L

D

D

L

I

N

T

G

A

A

S

F

V

E

E

G

K

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

G
|
G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

S

I

I

G
x
N

T
|
T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G

A

L

I

D
|
D

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T

T

D

S

I

I

A

D

K

R

D
|
D

G
|
G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

active site: N11 (≠ R16), D130 (= D130)
binding phosphate ion: G82 (= G83), N103 (≠ G104), T104 (= T105), D176 (= D172), G177 (= G173)

Sites not aligning to the query:

binding phosphate ion: 225

7qc8A Imidazole glycerol phosphate synthase subunit HisF (see paper)
30% identity, 80% coverage: 3:199/247 of query aligns to 6:203/250 of 7qc8A

query
sites
7qc8A

I

I

P

A

A

A

I

L

D
x
E

L

V

K

K

D

D

G

G

A

R

C

V

V

V

R

K

L

-

R

-

Q

-

G

G

L

T

M

N

D

F

D

E

A

N

T

L

V

R

F

D

S

S

D

G

D

D

P

P

V

A

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

V

L

F

V
x
H

D

D

L
x
H

N

T

G

A

A

S

F

V

E

E

G

K

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

S

I

I

G

N

T

T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G

A

L

I

D

D

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T

T

D

S

I

I

A

D

K

R

D

D

G

G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

binding zinc ion: E11 (≠ D8), H50 (≠ V50), H52 (≠ L52)

P60664 Imidazole glycerol phosphate synthase subunit HisF; IGP synthase cyclase subunit; IGP synthase subunit HisF; ImGP synthase subunit HisF; IGPS subunit HisF; EC 4.3.2.10 from Escherichia coli (strain K12) (see paper)
23% identity, 96% coverage: 3:238/247 of query aligns to 6:246/258 of P60664

query
sites
P60664

I

I

P

P

A

C

I

L

D

D

L

V

K

R

D

D

G

G

A

Q

C

V

V

V

R

K

L

-

R

-

Q

-

G

G

L

V

M

Q

D

F

D

R

A

N

T

H

V

E

F

I

S

I

D

G

D

D

P

I

V

V

A

P

M

L

A

A

A

K

K

R

W

Y

V

A

Q

E

A

E

G

G

C

A

R

D

R
x
E

L

L

H

V

L

F

V

Y

D

D

L

I

N

T

G

A

A

S

F

S

E

D

G

G

Q

R

P

V

V

V

N

D

G

K

E

S

V

W

V

V

T

S

A

R

I

V

A

A

K

E

R

-

Y

V

P

I

D

D

L

I

P

P

I

F

Q

C

I

V

G

A

G

G

I

I

R

K

T

S

L

L

E

E

T

D

I

A

E

A

H

K

Y

I

V

L

R

S

A

F

G

G

V

A

S

D

Y

K

V

I

I

S

I

I

G

N

T

S

K

P

A

A

V

L

K

A

E

D

P

P

E

T

F

L

V

I

T

T

E

R

A

L

C

A

R

D

A

R

F

F

P

G

G

V

K
x
Q

V
x
C

I

I

V

V

-

V

-

G

-

I

-

D

-

T

G

W

L

Y

D

D

A

A

K

E

D

T

G

G

-

K

-

Y

-

H

-

V

-

N

-

Q

F

Y

V

T

A

G

T

D

D

E

G

S

W

R

A

T

E

R

V

V

S

T

S

Q

V

W

Q

E

A

T

V

L

D

D

L

W

A

V

R

Q

R

E

F

V

E

Q

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

V

Y

L

T

N

D

M

I

M

A

N

K

Q

D

D

G

G

M

V

M

R

Q

N

G

G

C

Y

N

D

V

L

E

E

A

Q

T

L

V

K

A

K

L

V

A

R

N

E

A

V

S

C

R

H

I

V

P

P

V

L

I

I

A

A

S

S

G

G

I

-

H

-

N

-

I

A

G

G

D

T

I

M

Q

E

K

H

L

F

L

L

D

E

T

A

-

F

N

R

T

D

P

A

G

D

I

V

I

D

G

G

A

A

I

L

T

A

G

A

R

S

A

V

I

F

Y

H

E

K

G

Q

T

I

L

I

D

N

V

I

A

G

E

E

A

L

Q

K

A

A

E46 (≠ R46) mutation to A: Loss of activity.; mutation to G: Loss of IGP synthase activity. Weak IGP synthase and HisH activities in vitro.
Q123 (≠ K124) mutation to A: Decrease in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.
C124 (≠ V125) mutation to A: No change in activity.; mutation to R: Loss of IGP synthase activity. Weak HisH activity in vitro.

Sites not aligning to the query:

5 R→A: Loss of activity.; R→H: Loss of IGP synthase activity. Weak IGP synthase activity and reduced HisH activity in vitro.

2wjzE Crystal structure of (hish) k181a y138a mutant of imidazoleglycerolphosphate synthase (hish hisf) which displays constitutive glutaminase activity (see paper)
29% identity, 80% coverage: 3:199/247 of query aligns to 6:189/237 of 2wjzE

query
sites
2wjzE

I

I

P

A

A

C

I

L

D
|
D

L

V

K

K

D

D

G

G

A

R

C

V

V

V

R

-

L

-

R

-

Q

-

G

-

L

-

M

-

D

-

A

-

T

-

V

-

F

-

S

-

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

V

L

F

V

L

D

D

L

I

N

T

G

A

A

K

F

-

E

-

G

-

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

G
|
G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

S

I

I

G
x
N

T
|
T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G

A

L

I

D
|
D

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T

T

D

S

I

I

A

D

K

R

D

D

G

G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S

S

G

G

active site: D11 (= D8), D116 (= D130)
binding phosphate ion: G68 (= G83), N89 (≠ G104), T90 (= T105)

5d2tA Directed evolutionary changes in kemp eliminase ke07 - crystal 3 wild type
27% identity, 95% coverage: 3:237/247 of query aligns to 4:236/251 of 5d2tA

query
sites
5d2tA

I

I

P

A

A

A

I

L

D

I

L

V

K

K

D

D

G

G

A

R

C

V

V

V

R

K

L

G

R

S

-

N

-

F

Q

E

G

N

L

L

M

R

D

D

D

-

A

-

T

-

V

-

F

-

S

S

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

S

L

F

V
x
W

D

D

L

I

N

T

G

A

A

S

F

V

E

E

G

K

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I
x
E

I

I

G

N

T

T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G
x
Y

L

I

D

A

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

V

Y

L

T

G

D

S

I

I

A

D

K

R

D

L

G

G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S

H

G

G

I

-

H

-

N

-

I

A

G

G

D

K

I

M

Q

E

K

H

L

F

L

L

D

E

T

A

N

F

T

L

P

A

G

G

I

A

I

D

G

A

A

A

I
x
K

T

A

G

N

R

S

A

V

I

L

Y

H

E

F

G

R

T

E

L

I

D

D

V

V

A

R

E

E

A

L

Q

K

binding 5-nitro-2-oxidanyl-benzenecarbonitrile: W48 (≠ V50), E99 (≠ I102), Y126 (≠ G128), K220 (≠ I221)

4ewnD Structure of hisf-d130v+d176v with bound rcdrp (see paper)
29% identity, 80% coverage: 3:199/247 of query aligns to 5:196/243 of 4ewnD

query
sites
4ewnD

I

I

P

A

A

C

I

L

D

D

L

V

K

K

D

D

G

G

A

R

C

V

V

V

R

K

L

-

R

-

Q

-

G

G

L

T

M

N

D

F

D

E

A

N

T

L

V

R

F

D

S

S

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

V

L

F

V

L

D

D

L

I

N

T

G

K

A

T

F

M

E

L

G

-

Q

-

P

-

V

-

N

-

G

-

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G

G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

S

I

I

G

N

T

T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G

A

L

I

D
x
V

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

L

Y

L

T

T

D

S

I

I

A

D

K

R

D

V

G
|
G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S
|
S

G
|
G

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: V123 (≠ D130), G170 (= G173), S194 (= S197), G195 (= G198), G196 (= G199)

Sites not aligning to the query:

binding 1-(O-carboxy-phenylamino)-1-deoxy-D-ribulose-5-phosphate: 215, 216, 217, 218

6dnjA Directed evolutionary changes in kemp eliminase ke07 - crystal 28 round 5 (see paper)
26% identity, 94% coverage: 6:237/247 of query aligns to 8:237/250 of 6dnjA

query
sites
6dnjA

A

A

I

L

D

I

L

M

K

K

D

D

G

G

A

R

C

V

V

V

R

K

L

G

R

S

-

N

-

F

Q

E

G

N

L

L

M

R

D

D

D

-

A

-

T

-

V

-

F

-

S

S

D

G

D

D

P

P

V

V

A

E

M

L

A

G

A

K

K

F

W

Y

V

S

Q

E

A

I

G

G

C

I

R

D

R

E

L

L

H

S

L

F

V
x
W

D

D

L

I

N

T

G

A

A

S

F

V

E

E

G

K

Q

R

P

K

V

T

N

M

G

L

E

E

V

L

V

V

T

E

A

K

I

V

A

A

K

E

R

Q

Y

I

P

-

D

D

L

I

P

P

I

F

Q

T

I

V

G
|
G

G

G

I

I

R

H

T

D

L

F

E

E

T

T

I

A

E

S

H

E

Y

L

V

I

R

L

A

R

G

G

V

A

S

D

Y

K

V

V

I

E

I

I

G
x
N

T

T

K

A

A

A

V

V

K

E

E

N

P

P

E

S

F

L

V

I

T

T

E

Q

A

I

C

A

R

Q

A

T

F

F

P

G

G

S

K

Q

-

A

V

V

I

V

V

V

G
x
Y

L

I

D

A

A

A

K

K

-

R

-

V

D

D

G

G

-

E

-

F

F

M

V

V

A

F

T

T

D

Y

G

S

W

G

A

K

E

K

V

N

S

T

S

G

V

I

Q

L

A

L

V

R

D

D

L

W

A

V

R

V

R

E

F

V

E

E

A

K

D

R

G

G

V

A

S

G

A

E

I

I

V

V

Y

L

T

G

D

S

I

I

A

D

K

R

D

L

G

G

M

T

M

K

Q

S

G

G

C

Y

N

D

V

T

E

E

A

M

T

I

V

R

A

F

L

V

A

R

N

P

A

L

S

T

R

T

I

L

P

P

V

I

I

I

A

A

S

H

G

R

G

G

I

-

H

-

N

-

I

A

G

G

D

K

I

M

Q

E

K

H

L

F

L

L

D

E

T

A

N

F

T

L

P

A

G

G

I

A

I

D

G

A

A

A

I

K

T

A

G

D

R

S

A

V

I

F

Y

H

E

F

G

R

T

E

L

I

D

D

V

V

A

R

E

E

A

L

Q

K

binding 5-nitro-1,2-benzoxazole: W49 (≠ V50), G79 (= G81), G80 (= G82), N102 (≠ G104), Y127 (≠ G128)

Query Sequence

>WP_011915091.1 NCBI__GCF_000013785.1:WP_011915091.1
MLIIPAIDLKDGACVRLRQGLMDDATVFSDDPVAMAAKWVQAGCRRLHLVDLNGAFEGQP
VNGEVVTAIAKRYPDLPIQIGGGIRTLETIEHYVRAGVSYVIIGTKAVKEPEFVTEACRA
FPGKVIVGLDAKDGFVATDGWAEVSSVQAVDLARRFEADGVSAIVYTDIAKDGMMQGCNV
EATVALANASRIPVIASGGIHNIGDIQKLLDTNTPGIIGAITGRAIYEGTLDVAEAQALC
DLKLKDE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory