SitesBLAST
Comparing WP_011937525.1 NCBI__GCF_000016745.1:WP_011937525.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
3pefA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter metallireducens in complex with NADP+ (see paper)
76% identity, 100% coverage: 2:287/287 of query aligns to 2:287/287 of 3pefA
- binding glycerol: D67 (= D67), G123 (= G123), K171 (= K171), N175 (= N175), M178 (= M178), L203 (= L203), G207 (≠ D207), N213 (= N213), A217 (≠ K217), F232 (= F232), H236 (= H236), K239 (= K239), R242 (= R242), R269 (≠ K269)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), I11 (= I11), M12 (= M12), N31 (= N31), R32 (= R32), S33 (= S33), K36 (= K36), M64 (= M64), L65 (= L65), A66 (≠ S66), A70 (= A70), E73 (≠ A73), T96 (= T96), V121 (= V121), G123 (= G123), S124 (= S124), A231 (= A231), F232 (= F232), H236 (= H236), K239 (= K239)
3pduA Crystal structure of gamma-hydroxybutyrate dehydrogenase from geobacter sulfurreducens in complex with NADP+ (see paper)
66% identity, 100% coverage: 1:286/287 of query aligns to 1:286/287 of 3pduA
- binding glycerol: R242 (= R242), E246 (≠ G246), E246 (≠ G246), R250 (≠ W250)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G8 (= G8), G10 (= G10), I11 (= I11), M12 (= M12), N31 (= N31), R32 (= R32), N33 (≠ S33), M64 (= M64), L65 (= L65), A66 (≠ S66), A70 (= A70), T96 (= T96), V121 (= V121), G123 (= G123), T124 (≠ S124), K171 (= K171), S231 (≠ A231), F232 (= F232), P233 (= P233), H236 (= H236), K239 (= K239)
2uyyA Structure of the cytokine-like nuclear factor n-pac
51% identity, 98% coverage: 3:284/287 of query aligns to 8:289/292 of 2uyyA
- binding [(2r,3r,4r,5r)-5-(6-amino-9h-purin-9-yl)-3-hydroxy-4-(phosphonooxy)tetrahydrofuran-2-yl]methyl [(2r,3s,4s)-3,4-dihydroxytetrahydrofuran-2-yl]methyl dihydrogen diphosphate: G15 (= G10), L16 (≠ I11), M17 (= M12), N36 (= N31), R37 (= R32), T38 (≠ S33), V70 (≠ L65), S71 (= S66), A75 (= A70), T101 (= T96), F237 (= F232), Y238 (≠ P233), Y241 (≠ H236), K244 (= K239)
Q49A26 Cytokine-like nuclear factor N-PAC; NPAC; 3-hydroxyisobutyrate dehydrogenase-like protein; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Nuclear protein of 60 kDa; Nucleosome-destabilizing factor; hNDF; Putative oxidoreductase GLYR1 from Homo sapiens (Human) (see 3 papers)
51% identity, 98% coverage: 3:284/287 of query aligns to 269:550/553 of Q49A26
- 271:285 (vs. 5:19, 67% identical) binding NAD(+)
- T362 (= T96) binding NAD(+)
- M437 (≠ K171) mutation to K: Loss of tetramerization and protein stability.; mutation to N: No effect on tetramerization or protein stability.
- P496 (≠ T230) to L: decreased interaction with GATA4; decreased synergistic activation of GATA4 target genes transcription; detrimental effect on cardiomyocyte differentiation
- K505 (= K239) binding NAD(+)
Sites not aligning to the query:
- 214 D→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
- 214:217 Interaction with histone H3
- 216 H→A: Slightly reduced stimulation of KDM1B demethylase activity, but normal KDM1B-binding.
- 216:225 Interaction with KDM1B
- 217 Required to promote KDM1B demethylase activity toward histone H3K4me1 and H3K4me2; F→A: Abolished stimulation of KDM1B demethylase activity, reduced affinity for histone H3 of the dimer with KDM1B, but normal KDM1B-binding.
- 219 H→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-223.
- 220:222 FLL→AAA: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity.
- 223 S→A: Impaired KDM1B-binding and abolished stimulation of KDM1B demethylase activity; when associated with A-219.
Q922P9 Cytokine-like nuclear factor N-PAC; NPAC; Glyoxylate reductase 1 homolog; Nuclear protein NP60; Putative oxidoreductase GLYR1 from Mus musculus (Mouse) (see paper)
50% identity, 98% coverage: 3:284/287 of query aligns to 268:543/546 of Q922P9
- P489 (≠ T230) mutation to L: Mutant animals are born at expected Mendelian ratios. 54% mutants display postnatal lethality between days 0 and 1. They show centricular septal defects.
Q8T079 Cytokine-like nuclear factor N-PAC; NPAC; Glyoxylate reductase 1 homolog; Nuclear protein NP60 homolog; Nucleosome-destabilizing factor; Putative oxidoreductase GLYR1 homolog from Drosophila melanogaster (Fruit fly) (see paper)
39% identity, 97% coverage: 4:282/287 of query aligns to 318:597/602 of Q8T079
Sites not aligning to the query:
- 8 modified: Phosphoserine
- 10 modified: Phosphoserine
- 224 modified: Phosphoserine
- 228 modified: Phosphoserine
- 243 modified: Phosphoserine
3w6zA Crystal structure of NADP bound l-serine 3-dehydrogenase (k170m) from hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
34% identity, 97% coverage: 3:281/287 of query aligns to 15:293/296 of 3w6zA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G8), L21 (= L9), G22 (= G10), I23 (= I11), M24 (= M12), N43 (= N31), R44 (= R32), T45 (≠ S33), K48 (= K36), V77 (≠ L65), S78 (= S66), D82 (≠ A70), Q85 (≠ A73), V133 (= V121), F244 (= F232), K245 (≠ P233), H248 (= H236), K251 (= K239)
3ws7A The 1.18 a resolution structure of l-serine 3-dehydrogenase complexed with NADP+ and sulfate ion from the hyperthermophilic archaeon pyrobaculum calidifontis (see paper)
35% identity, 97% coverage: 3:281/287 of query aligns to 15:290/293 of 3ws7A
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G20 (= G8), L21 (= L9), G22 (= G10), I23 (= I11), M24 (= M12), N43 (= N31), R44 (= R32), T45 (≠ S33), K48 (= K36), M76 (= M64), V77 (≠ L65), S78 (= S66), D82 (≠ A70), Q85 (≠ A73), V133 (= V121), F241 (= F232), K242 (≠ P233), H245 (= H236), K248 (= K239)
- binding sulfate ion: T134 (≠ S122), G135 (= G123), K183 (= K171)
8z9fA Crystal structure of glyoxylate reductase from acetobacter aceti in complex with nadh
32% identity, 99% coverage: 3:287/287 of query aligns to 10:289/293 of 8z9fA
- binding 1,4-dihydronicotinamide adenine dinucleotide: F16 (≠ L9), G17 (= G10), A18 (≠ I11), M19 (= M12), P39 (vs. gap), C67 (≠ M64), V68 (≠ L65), P69 (≠ S66), A73 (= A70), S77 (≠ V74), S99 (≠ T96), G126 (= G123), S127 (= S124), Q234 (≠ A231), F235 (= F232)
8z9gA Crystal structure of glyoxylate reductase from acetobacter aceti in complex with NADPH
32% identity, 99% coverage: 3:287/287 of query aligns to 9:288/291 of 8z9gA
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F15 (≠ L9), G16 (= G10), A17 (≠ I11), M18 (= M12), T37 (≠ N31), P38 (vs. gap), S39 (vs. gap), C66 (≠ M64), V67 (≠ L65), P68 (≠ S66), A72 (= A70), S76 (≠ V74), S98 (≠ T96), S126 (= S124), K173 (= K171), Q233 (≠ A231), F234 (= F232), K241 (= K239)
P29266 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Rattus norvegicus (Rat) (see paper)
28% identity, 99% coverage: 5:287/287 of query aligns to 42:331/335 of P29266
- D68 (≠ N31) mutation to R: Decrease of activity with NAD, increase of activity with NADP.
- K208 (= K171) mutation K->A,H,N,R: Complete loss of activity.
- N212 (= N175) mutation to Q: Decrease in activity.
2i9pB Crystal structure of human hydroxyisobutyrate dehydrogenase complexed with NAD+
28% identity, 97% coverage: 5:283/287 of query aligns to 4:289/296 of 2i9pB
- binding nicotinamide-adenine-dinucleotide: G9 (= G10), N10 (≠ I11), M11 (= M12), Y29 (≠ W30), D30 (≠ N31), V31 (≠ R32), M63 (= M64), L64 (= L65), P65 (≠ S66), T95 (= T96), V120 (= V121), G122 (= G123), F238 (= F232), K245 (= K239)
5je8B The crystal structure of bacillus cereus 3-hydroxyisobutyrate dehydrogenase in complex with NAD (see paper)
28% identity, 100% coverage: 1:287/287 of query aligns to 3:291/294 of 5je8B
P31937 3-hydroxyisobutyrate dehydrogenase, mitochondrial; HIBADH; EC 1.1.1.31 from Homo sapiens (Human) (see paper)
28% identity, 99% coverage: 5:287/287 of query aligns to 43:332/336 of P31937
- LP 103:104 (≠ LS 65:66) binding NAD(+)
- N108 (≠ A70) binding NAD(+)
- T134 (= T96) binding NAD(+)
- K284 (= K239) binding NAD(+)
Sites not aligning to the query:
- 1:36 modified: transit peptide, Mitochondrion
- 40:68 binding NAD(+)
P0A9V8 3-sulfolactaldehyde reductase; SLA reductase; 4-hydroxybutyrate dehydrogenase; Gamma-hydroxybutyrate dehydrogenase; GHBDH; Succinic semialdehyde reductase; SSA reductase; EC 1.1.1.373; EC 1.1.1.61 from Escherichia coli (strain K12)
29% identity, 100% coverage: 1:286/287 of query aligns to 1:287/298 of P0A9V8
- QM 11:12 (≠ IM 11:12) binding NAD(+)
- D31 (≠ N31) binding NAD(+)
- L65 (= L65) binding NAD(+)
- T96 (= T96) binding NAD(+)
- G122 (≠ S122) mutation to S: 25-fold decrease in catalytic efficiency with SLA as substrate. 5-fold decrease in catalytic efficiency with NADH as substrate.
- R123 (≠ G123) binding 2,3-dihydroxypropane-1-sulfonate; mutation to G: 130-fold decrease in catalytic efficiency with SLA as substrate. 3-fold decrease in catalytic efficiency with NADH as substrate.
- T124 (≠ S124) mutation to G: 230-fold decrease in catalytic efficiency with SLA as substrate. 12-fold decrease in catalytic efficiency with NADH as substrate.
- NNYMS 174:178 (≠ VNMVM 174:178) binding 2,3-dihydroxypropane-1-sulfonate
- K240 (= K239) binding NAD(+)
6smyA Crystal structure of sla reductase yihu from e. Coli with nadh and product dhps
29% identity, 99% coverage: 4:286/287 of query aligns to 3:286/294 of 6s