SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011938055.1 NCBI__GCF_000016745.1:WP_011938055.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

5xu1B Structure of a non-canonical abc transporter from streptococcus pneumoniae r6 (see paper)
55% identity, 93% coverage: 3:225/241 of query aligns to 2:224/226 of 5xu1B

query
sites
5xu1B

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binding magnesium ion: G45 (= G46), K46 (= K47), S47 (= S48)

5lilA Structure of aggregatibacter actinomycetemcomitans macb bound to atpys (p21) (see paper)
49% identity, 97% coverage: 4:236/241 of query aligns to 3:237/615 of 5lilA

query
sites
5lilA

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binding adenosine-5'-triphosphate: F13 (≠ Y14), V22 (≠ A23), S42 (= S43), G43 (= G44), G45 (= G46), K46 (= K47), S47 (= S48), T48 (= T49), Q92 (= Q93), K136 (≠ R137), Q143 (= Q144), S145 (= S146), G147 (= G148), Q148 (= Q149)
binding magnesium ion: S47 (= S48), Q92 (= Q93)

5lj7A Structure of aggregatibacter actinomycetemcomitans macb bound to atp (p21) (see paper)
49% identity, 97% coverage: 4:236/241 of query aligns to 3:237/592 of 5lj7A

query
sites
5lj7A

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binding adenosine-5'-triphosphate: V22 (≠ A23), S42 (= S43), G43 (= G44), G45 (= G46), K46 (= K47), S47 (= S48), T48 (= T49), Q92 (= Q93), K136 (≠ R137), Q143 (= Q144), S145 (= S146), G147 (= G148), Q148 (= Q149)
binding magnesium ion: S47 (= S48), Q92 (= Q93)

P75831 Macrolide export ATP-binding/permease protein MacB; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
51% identity, 93% coverage: 1:224/241 of query aligns to 1:224/648 of P75831

query
sites
P75831

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N

K47 (= K47) mutation to L: Lack of activity.
D169 (= D169) mutation to N: Lack of activity.

1f3oA Crystal structure of mj0796 atp-binding cassette (see paper)
52% identity, 90% coverage: 4:219/241 of query aligns to 1:221/232 of 1f3oA

query
sites
1f3oA

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G

binding adenosine-5'-diphosphate: Y11 (= Y14), G41 (= G44), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49)

1l2tA Dimeric structure of mj0796, a bacterial abc transporter cassette (see paper)
51% identity, 90% coverage: 4:219/241 of query aligns to 1:221/230 of 1l2tA

query
sites
1l2tA

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T

T

S

E

G

G

E

E

Y

V

L

Y

L

I

E

D

E

N

V

I

N

K

V

T

G

N

Q

D

L

L

S

D

R

D

N

D

E

E

L

L

A

T

E

K

I

I

R

R

N

R

R

D

K

K

L

I

G

G

F

F

V

V

F

F

Q

Q

G

Q

F

F

N

N

L

L

L

I

A

P

R

L

T

L

T

T

A

A

R

L

E

E

N

N

V

V

E

E

L

L

P

P

L

L

V

I

Y

F

A

K

H

Y

-

R

-

G

-

A

V

M

P

S

A

G

A

E

E

E

R

R

R

R

E

K

R

R

A

A

H

L

T

E

S

C

L

L

A

K

R

M

V

A

G

E

L

L

A

E

G

E

R

R

-
x
F

E

A

G

N

H

H

Y

K

S

P

N

N

Q
|
Q

L

L

S
|
S

G

G

G
|
G

Q
|
Q

Q

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

N

E

N

P

P

A

P

I

I

I

I

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

K

T

T

A

G

V

E

E

K

I

I

M

M

A

Q

I

L

F

L

Q

K

E

K

L

L

N

N

R

E

Q

E

-

D

G

G

I

K

T

T

I

V

I

V

M

V

V

V

T

T

H
|
H

E

D

P

I

E

N

V

V

A

A

A

R

F

F

T

G

G

E

R

R

H

I

I

I

I

Y

F

L

R

K

D

D

G

G

binding adenosine-5'-triphosphate: Y11 (= Y14), S40 (= S43), G41 (= G44), S42 (= S45), G43 (= G46), K44 (= K47), S45 (= S48), T46 (= T49), F138 (vs. gap), Q145 (= Q144), S147 (= S146), G149 (= G148), Q150 (= Q149), H204 (= H202)

5ws4A Crystal structure of tripartite-type abc transporter macb from acinetobacter baumannii (see paper)
49% identity, 93% coverage: 4:227/241 of query aligns to 4:227/650 of 5ws4A

query
sites
5ws4A

V

L

V

L

K

E

I

V

T

S

D

N

V

L

T

V

K

R

V

E

Y

F

T

P

M

A

G

G

D

E

Q

S

R

T

V

I

E

Q

A

I

L

L

K

K

G

G

I

I

S

D

F

L

S

T

I

I

R

Y

R

E

G

G

E

E

F

L

V

V

A

A

I

I

M

V

G

G

A

Q

S
|
S

G
|
G

S

S

G

G

K

K

S
|
S

T

T

C

L

M

M

N

N

M

I

L

L

G

G

C

C

L

L

D

D

V

R

Q

P

T

T

S

S

G

G

E

S

Y

Y

L

K

L

V

E

N

E

G

V

Q

N

E

V

T

G

G

Q

K

L

L

S

E

R

P

N

D

E

Q

L

L

A

A

E

Q

I

L

R

R

N

R

R

E

K

Y

L

F

G

G

F

F

V

I

F

F

Q

Q

G

R

F

Y

N

H

L

L

L

L

A

G

R

D

T

L

T

S

A

A

R

E

E

G

N

N

V

V

E

E

L

V

P

P

L

A

V

V

Y

Y

A

A

H

G

V

V

P

T

A

P

A

A

E

D

R

R

R

K

E

Q

R

R

A

A

H

T

T

A

S

L

L

L

A

T

R

E

V

L

G

G

L

L

A

G

G

T

R

K

E

T

G

Q

H

N

Y

R

S

P

N

S

Q

Q

L

L

S

S

G

G

G

G

Q

Q

Q

Q

Q

Q

R

R

V

V

A

S

I

I

A

A

R

R

A

A

L

L

V

M

N

N

E

G

P

G

A

D

I

V

I

I

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

H

T

S

A

G

V

V

E

E

I

V

M

M

A

R

I

I

F

L

Q

R

E

E

L

L

N

N

R

A

Q

A

G

G

I

H

T

T

I

I

I

I

M

L

V

V

T

T

H

H

E

D

P

M

E

Q

V

V

A

A

A

K

F

N

T

A

G

T

R

R

H

I

I

I

I

E

F

I

R

S

D

D

G

G

V

E

I

I

I

I

S

S

D

D

T

R

R

P

N

N

binding 5'-o-[(r)-hydroxy(thiophosphonooxy)phosphoryl]adenosine: S43 (= S43), G44 (= G44), S48 (= S48)

2pclA Crystal structure of abc transporter with complex (aq_297) from aquifex aeolicus vf5
46% identity, 92% coverage: 3:224/241 of query aligns to 2:219/223 of 2pclA

query
sites
2pclA

E

E

V

I

V

L

K

R

I

A

T

E

D

N

V

I

T

K

K

K

V

V

Y

I

T

-

M

-

G

-

D

-

Q

R

R

G

V

Y

E

E

A

I

L

L

K

K

G

G

I

I

S

S

F

L

S

S

I

V

R

K

R

K

G

G

E

E

F

F

V

V

A

S

I

I

M

I

G

G

A

A

S

S

G

G

S

S

G

G

K

K

S

S

T

T

C

L

M

L

N

Y

M

I

L

L

G

G

C

L

L

L

D

D

V

A

Q

P

T

T

S

E

G

G

E

K

Y

V

L

F

L

L

E

E

E

G

V

K

N

E

V

V

G

D

Q

Y

L

T

S

N

R

E

N

K

E

E

L

L

A

S

E

L

I

L

R

R

N

N

R

R

K

K

L

L

G

G

F

F

V

V

F

F

Q

Q

G

F

F

H

N

Y

L

L

L

I

A

P

R

E

T

L

T

T

A

A

R

L

E

E

N

N

V

V

E

I

L

V

P

P

L

M

V

L

Y

K

A

M

H

G

V

K

P

P

A

K

A

K

E

E

R

A

R

K

E

E

R

R

A

G

H

E

T

Y

S

L

L

L

A

S

R

E

V

L

G

G

L

L

A

G

G

D

R

K

E

L

G

S

H

R

Y

K

S

P

N

Y

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

Q

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

N

E

E

P

P

A

I

I

L

I

L

L

F

A

A

D

D

E
|
E

P
|
P

T
|
T

G

G

N

N

L

L

D

D

S

S

K

A

T

N

A

T

V

K

E

R

I

V

M

M

A

D

I

I

F

F

Q

L

E

K

L

I

N

N

R

E

Q

G

G

G

I

T

T

S

I

I

I

V

M

M

V

V

T
|
T

H

H

E

E

P

R

E

E

V

L

A

A

A

E

F

L

T

T

G

H

R

R

H

T

I

L

I

E

F

M

R

K

D

D

G

G

V

K

I

V

I

V

S

G

D

E

binding magnesium ion: E165 (= E170), P166 (= P171), T167 (= T172), T196 (= T201)

P75957 Lipoprotein-releasing system ATP-binding protein LolD; EC 7.6.2.- from Escherichia coli (strain K12) (see paper)
44% identity, 92% coverage: 4:224/241 of query aligns to 5:226/233 of P75957

query
sites
P75957

V

L

V

L

K

Q

I

C

T

D

D

N

V

L

T

C

K

K

V

R

Y

Y

T

Q

M

E

G

G

D

S

Q

V

R

Q

V

T

E

D

A

V

L

L

K

H

G

N

I

V

S

S

F

F

S

S

I

V

R

G

R

E

G

G

E

E

F

M

V

M

A

A

I

I

M

V

G
|
G

A

S

S

S

G

G

S

S

G

G

K

K

S

S

T

T

C

L

M

L

N

H

M

L

L

L

G

G

C

G

L

L

D

D

V

T

Q

P

T

T

S

S

G

G

E

D

Y

V

L

I

L

F

E

N

E

G

V

Q

N

P

V

M

G

S

Q

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

R

Q

K

K

L

L

G

G

F

F

V

I

F

Y

Q

Q

G

F

F

H

N

H

L

L

L

L

A

P

R

D

T

F

T

T

A

A

R

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

V

L

Y

I

A

G

H

K

V

K

P

K

A

P

A

A

E

E

R

I

R

N

E

S

R

R

A

A

H

L

T

E

S

M

L

L

A

K

R

A

V

V

G

G

L

L

A

D

G

H

R

R

E

A

G

N

H

H

Y

R

S

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

E

N

P

P

A

R

I

L

I

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

K

R

T

N

A

A

V

D

E

S

I

I

M

F

A

Q

I

L

F

L

Q

G

E

E

L

L

N

N

R

R

-

L

Q

Q

G

G

I

T

T

A

I

F

I

L

M

V

V

V

T

T

H

H

E

D

P

L

E

Q

V

L

A

A

A

K

F

R

T

M

G

S

R

R

H

Q

I

L

I

E

F

M

R

R

D

D

G

G

V

R

I

L

I

T

S

A

D

E

G42 (= G41) mutation to D: Loss of lipoprotein release when overexpressed.

7mdyC Lolcde nucleotide-bound
44% identity, 92% coverage: 4:224/241 of query aligns to 2:223/226 of 7mdyC

query
sites
7mdyC

V

L

V

L

K

Q

I

C

T

D

D

N

V

L

T

C

K

K

V

R

Y
|
Y

T

Q

M

E

G

G

D

S

Q

V

R

Q

V

T

E

D

A

V

L

L

K

H

G

N

I

V

S

S

F

F

S

S

I

V

R

G

R

E

G

G

E

E

F

M

V

M

A

A

I

I

M

V

G

G

A

S

S

S

G
|
G

S
|
S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

L

N

H

M

L

L

L

G

G

C

G

L

L

D

D

V

T

Q

P

T

T

S

S

G

G

E

D

Y

V

L

I

L

F

E

N

E

G

V

Q

N

P

V

M

G

S

Q

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

R

Q

K

K

L

L

G

G

F

F

V

I

F

Y

Q
|
Q

G

F

F

H

N

H

L

L

L

L

A

P

R

D

T

F

T

T

A

A

R

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

V

L

Y

I

A

G

H

K

V

K

P

K

A

P

A

A

E

E

R

I

R

N

E

S

R

R

A

A

H

L

T

E

S

M

L

L

A

K

R

A

V

V

G

G

L

L

A

D

G

H

R

R

E

A

G

N

H
|
H

Y

R

S

P

N

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

E

N

P

P

A

R

I

L

I

V

L

L

A

A

D

D

E
|
E

P

P

T

T

G

G

N
|
N

L

L

D

D

S

A

K

R

T

N

A

A

V

D

E

S

I

I

M

F

A

Q

I

L

F

L

Q

G

E

E

L

L

N

N

R

R

-

L

Q

Q

G

G

I

T

T

A

I

F

I

L

M

V

V

V

T

T

H
|
H

E

D

P

L

E

Q

V

L

A

A

A

K

F

R

T

M

G

S

R

R

H

Q

I

L

I

E

F

M

R

R

D

D

G

G

V

R

I

L

I

T

S

A

D

E

binding adp orthovanadate: Y12 (= Y14), G42 (= G44), S43 (= S45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49), Q91 (= Q93), H138 (= H140), E142 (≠ Q144), S144 (= S146), G145 (= G147), G146 (= G148), E168 (= E170), N172 (= N174), H201 (= H202)
binding magnesium ion: S46 (= S48), Q91 (= Q93)

7arlD Lolcde in complex with lipoprotein and adp (see paper)
45% identity, 90% coverage: 4:219/241 of query aligns to 2:218/222 of 7arlD

query
sites
7arlD

V

L

V

L

K

Q

I

C

T

D

D

N

V

L

T

C

K

K

V

R

Y

Y

T

Q

M

E

G

G

D

S

Q

V

R

Q

V

T

E

D

A

V

L

L

K

H

G

N

I

V

S

S

F

F

S

S

I

V

R

G

R

E

G

G

E

E

F

M

V

M

A

A

I

I

M

V

G

G

A

S

S

S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T

T

C

L

M

L

N

H

M

L

L

L

G

G

C

G

L

L

D

D

V

T

Q

P

T

T

S

S

G

G

E

D

Y

V

L

I

L

F

E

N

E

G

V

Q

N

P

V

M

G

S

Q

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

R

Q

K

K

L

L

G

G

F

F

V

I

F

Y

Q

Q

G

F

F

H

N

H

L

L

L

L

A

P

R

D

T

F

T

T

A

A

R

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

V

L

Y

I

A

G

H

K

V

K

P

K

A

P

A

A

E

E

R

I

R

N

E

S

R

R

A

A

H

L

T

E

S

M

L

L

A

K

R

A

V

V

G

G

L

L

A

D

G

H

R

R

E

A

G

N

H

H

Y

R

S

P

N

S

Q

E

L

L

S

S

G

G

G

G

Q

E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

E

N

P

P

A

R

I

L

I

V

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

A

K

R

T

N

A

A

V

D

E

S

I

I

M

F

A

Q

I

L

F

L

Q

G

E

E

L

L

N

N

R

R

-

L

Q

Q

G

G

I

T

T

A

I

F

I

L

M

V

V

V

T

T

H

H

E

D

P

L

E

Q

V

L

A

A

A

K

F

R

T

M

G

S

R

R

H

Q

I

L

I

E

F

M

R

R

D

D

G

G

binding adenosine-5'-diphosphate: G42 (= G44), G44 (= G46), K45 (= K47), S46 (= S48)

9gvkD Cryo-em structure of endogenous atp-bound lolcde with lold-e171q mutations in nanodiscs (see paper)
44% identity, 92% coverage: 4:224/241 of query aligns to 3:224/226 of 9gvkD

query
sites
9gvkD

V

L

V

L

K

Q

I

C

T

D

D

N

V

L

T

C

K

K

V

R

Y
|
Y

T

Q

M

E

G

G

D

S

Q

V

R

Q

V

T

E

D

A

V

L

L

K

H

G

N

I

V

S

S

F

F

S

S

I

V

R

G

R

E

G

G

E

E

F

M

V

M

A

A

I

I

M

V

G

G

A

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

L

N

H

M

L

L

L

G

G

C

G

L

L

D

D

V

T

Q

P

T

T

S

S

G

G

E

D

Y

V

L

I

L

F

E

N

E

G

V

Q

N

P

V

M

G

S

Q

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

R

Q

K

K

L

L

G

G

F

F

V

I

F

Y

Q
|
Q

G

F

F

H

N

H

L

L

L

L

A

P

R

D

T

F

T

T

A

A

R

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

V

L

Y

I

A

G

H

K

V

K

P

K

A

P

A

A

E

E

R

I

R

N

E

S

R

R

A

A

H

L

T

E

S

M

L

L

A

K

R

A

V

V

G

G

L

L

A

D

G

H

R
|
R

E

A

G

N

H
|
H

Y

R

S

P

N

S

Q
x
E

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

E

N

P

P

A

R

I

L

I

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

S

A

K

R

T

N

A

A

V

D

E

S

I

I

M

F

A

Q

I

L

F

L

Q

G

E

E

L

L

N

N

R

R

-

L

Q

Q

G

G

I

T

T

A

I

F

I

L

M

V

V

V

T

T

H

H

E

D

P

L

E

Q

V

L

A

A

A

K

F

R

T

M

G

S

R

R

H

Q

I

L

I

E

F

M

R

R

D

D

G

G

V

R

I

L

I

T

S

A

D

E

binding adenosine-5'-triphosphate: Y13 (= Y14), S42 (= S43), G43 (= G44), G45 (= G46), K46 (= K47), S47 (= S48), T48 (= T49), Q92 (= Q93), R136 (= R137), H139 (= H140), E143 (≠ Q144), S145 (= S146), G146 (= G147), G147 (= G148), E148 (≠ Q149)
binding magnesium ion: S47 (= S48), Q92 (= Q93)

7v8iD Lolcd(e171q)e with bound amppnp in nanodiscs (see paper)
44% identity, 92% coverage: 4:224/241 of query aligns to 4:225/229 of 7v8iD

query
sites
7v8iD

V

L

V

L

K

Q

I

C

T

D

D

N

V

L

T

C

K

K

V

R

Y

Y

T

Q

M

E

G

G

D

S

Q

V

R

Q

V

T

E

D

A
x
V

L

L

K

H

G

N

I

V

S

S

F

F

S

S

I

V

R

G

R

E

G

G

E

E

F

M

V

M

A

A

I

I

M

V

G

G

A

S

S
|
S

G
|
G

S

S

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

L

N

H

M

L

L

L

G

G

C

G

L

L

D

D

V

T

Q

P

T

T

S

S

G

G

E

D

Y

V

L

I

L

F

E

N

E

G

V

Q

N

P

V

M

G

S

Q

K

L

L

S

S

R

S

N

A

E

A

L

K

A

A

E

E

I

L

R

R

N

N

R

Q

K

K

L

L

G

G

F

F

V

I

F

Y

Q
|
Q

G

F

F

H

N

H

L

L

L

L

A

P

R

D

T

F

T

T

A

A

R

L

E

E

N

N

V

V

E

A

L

M

P

P

L

L

V

L

Y

I

A

G

H

K

V

K

P

K

A

P

A

A

E

E

R

I

R

N

E

S

R

R

A

A

H

L

T

E

S

M

L

L

A

K

R

A

V

V

G

G

L

L

A

D

G

H

R
|
R

E

A

G

N

H
|
H

Y

R

S

P

N

S

Q
x
E

L

L

S
|
S

G

G

G
|
G

Q
x
E

Q

R

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

V

N

N

E

N

P

P

A

R

I

L

I

V

L

L

A

A

D

D

E

Q

P

P

T

T

G

G

N

N

L

L

D

D

S

A

K

R

T

N

A

A

V

D

E

S

I

I

M

F

A

Q

I

L

F

L

Q

G

E

E

L

L

N

N

R

R

-

L

Q

Q

G

G

I

T

T

A

I

F

I

L

M

V

V

V

T

T

H
|
H

E

D

P

L

E

Q

V

L

A

A

A

K

F

R

T

M

G

S

R

R

H

Q

I

L

I

E

F

M

R

R

D

D

G

G

V

R

I

L

I

T

S

A

D

E

binding phosphoaminophosphonic acid-adenylate ester: V23 (≠ A23), S43 (= S43), G44 (= G44), G46 (= G46), K47 (= K47), S48 (= S48), T49 (= T49), Q93 (= Q93), R137 (= R137), H140 (= H140), E144 (≠ Q144), S146 (= S146), G148 (= G148), E149 (≠ Q149), H203 (= H202)
binding magnesium ion: S48 (= S48), Q93 (= Q93)

8g4cB Bceabs atpgs high res tm (see paper)
40% identity, 90% coverage: 4:219/241 of query aligns to 3:219/248 of 8g4cB

query
sites
8g4cB

V

I

V

L

K

E

I

A

T

N

D

K

V

I

T

R

K

K

V

S

Y
|
Y

T

G

M

N

G

K

D

L

Q

N

R

K

V

Q

E

E

A

V

L

L

K

K

G

G

I

I

S

D

F

I

S

H

I

I

R

E

R

K

G

G

E

E

F

F

V

V

A

S

I

I

M

M

G

G

A

A

S
|
S

G

G

S
|
S

G

G

K

K

S
x
T

T

T

C

L

M

L

N

N

M

V

L

L

G

S

C

S

L

I

D

D

V

Q

Q

V

T

S

S

H

G

G

E

T

Y

I

L

H

L

I

E

N

E

G

V

N

N

D

V

M

G

T

Q

A

L

M

S

K

R

E

N

K

E

Q

L

L

A

A

E

E

I

F

R

R

N

K

R

Q

K

H

L

L

G

G

F

F

V

I

F

F

Q

Q

G

D

F

Y

N

N

L

L

L

L

A

D

R

T

T

L

T

T

A

V

R

K

E

E

N

N

V

I

E

L

L

L

P

P

L

L

V

S

Y

I

A

T

H

K

V

L

P

S

A

K

A

K

E

E

R

A

R

N

E

R

R

K

A

F

H

E

T

E

S

V

L

A

A

K

R

E

V

L

G

G

L

I

A

Y

G

E

R

L

E

R

G

D

H

K

Y

Y

S

P

N

N

Q

E

L

I

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

V

I

N

H

E

D

P

P

A

S

I

I

I

I

L

F

A

A

D
|
D

E

E

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

K

T

S

A

A

V

S

E

D

I

L

M

L

A

N

I

K

F

L

Q

S

E

Q

L

L

N

N

-

Q

R

K

Q

R

G

N

I

A

T

T

I

I

I

I

M

M

V

V

T

T

H

H

E

D

P

P

E

V

V

A

A

A

A

S

F

Y

T

C

G

G

R

R

H

V

I

I

I

F

F

I

R

K

D

D

G

G

binding phosphothiophosphoric acid-adenylate ester: Y13 (= Y14), S42 (= S43), S44 (= S45), T47 (≠ S48), D168 (= D169)

7tchB Bceab e169q variant atp-bound conformation (see paper)
39% identity, 90% coverage: 4:219/241 of query aligns to 2:218/245 of 7tchB

query
sites
7tchB

V

I

V

L

K

E

I

A

T

N

D

K

V

I

T

R

K

K

V

S

Y
|
Y

T

G

M

N

G

K

D

L

Q

N

R

K

V
x
Q

E

E

A
x
V

L

L

K

K

G

G

I

I

S

D

F

I

S

H

I

I

R

E

R

K

G

G

E

E

F

F

V

V

A

S

I

I

M

M

G

G

A

A

S
|
S

G
|
G

S
|
S

G

G

K
|
K

S
x
T

T

T

C

L

M

L

N

N

M

V

L

L

G

S

C

S

L

I

D

D

V

Q

Q

V

T

S

S

H

G

G

E

T

Y

I

L

H

L

I

E

N

E

G

V

N

N

D

V

M

G

T

Q

A

L

M

S

K

R

E

N

K

E

Q

L

L

A

A

E

E

I

F

R

R

N

K

R

Q

K

H

L

L

G

G

F

F

V

I

F

F

Q

Q

G

D

F

Y

N

N

L

L

L

L

A

D

R

T

T

L

T

T

A

V

R

K

E

E

N

N

V

I

E

L

L

L

P

P

L

L

V

S

Y

I

A

T

H

K

V

L

P

S

A

K

A

K

E

E

R

A

R

N

E

R

R

K

A

F

H

E

T

E

S

V

L

A

A

K

R

E

V

L

G

G

L

I

A

Y

G

E

R

L

E

R

G

D

H

K

Y

Y

S

P

N

N

Q
x
E

L

I

S

S

G
|
G

G
|
G

Q

Q

Q

K

Q

Q

R

R

V

T

A

S

I

A

A

G

R

R

A

A

L

F

V

I

N

H

E

D

P

P

A

S

I

I

I

I

L

F

A

A

D

D

E

Q

P

P

T

T

G

G

N

A

L

L

D

D

S

S

K

K

T

S

A

A

V

S

E

D

I

L

M

L

A

N

I

K

F

L

Q

S

E

Q

L

L

N

N

-

Q

R

K

Q

R

G

N

I

A

T

T

I

I

I

I

M

M

V

V

T

T

H

H

E

D

P

P

E

V

V

A

A

A

A

S

F

Y

T

C

G

G

R

R

H

V

I

I

I

F

F

I

R

K

D

D

G

G

binding adenosine-5'-triphosphate: Y12 (= Y14), Q19 (≠ V21), V21 (≠ A23), S41 (= S43), G42 (= G44), S43 (= S45), K45 (= K47), T46 (≠ S48), E142 (≠ Q144), G145 (= G147), G146 (= G148)

P30750 Methionine import ATP-binding protein MetN; EC 7.4.2.11 from Escherichia coli (strain K12) (see 3 papers)
39% identity, 84% coverage: 4:206/241 of query aligns to 1:203/343 of P30750

query
sites
P30750

V

M

V

I

K

K

I

L

T

S

D

N

V

I

T

T

K

K

V

V

Y

F

T

H

M

Q

G

G

D

T

Q

R

R

T

V

I

E

Q

A

A

L

L

K

N

G

N

I

V

S

S

F

L

S

H

I

V

R

P

R

A

G

G

E

Q

F

I

V

Y

A

G

I

V

M

I

G

G

A

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

M

C

L

V

G

N

C

L

L

L

D

E

V

R

Q

P

T

T

S

E

G

G

E

S

Y

V

L

L

L

V

E

D

E

G

V

Q

N

E

V

L

G

T

Q

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

E

K

I

A

R

R

N

-

R

R

K

Q

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

A

S

R

S

T

R

T

T

A

V

R

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

V

E

Y

L

A

D

H

N

V

T

P

P

A

K

A

D

E

E

R

V

R

K

E

R

R

R

A

V

H

T

T

E

S

L

L

L

A

S

R

L

V

V

G

G

L

L

A

G

G

D

R

K

E

H

G

D

H

S

Y

Y

S

P

N

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

S

E

N

P

P

A

K

I

V

I

L

L

L

A

C

D

D

E
|
E

P

A

T

T

G

S

N

A

L

L

D

D

S

P

K

A

T

T

A

T

V

R

E

S

I

I

M

L

A

E

I

L

F

L

Q

K

E

D

L

I

N

N

R

R

Q

R

-

L

G

G

I

L

T

T

I

I

I

L

M

L

V

I

T

T

H

H

E

E

P

M

E

D

V

V

40:46 (vs. 43:49, 86% identical) binding ATP
E166 (= E170) mutation to Q: Exhibits little ATPase activity.

Sites not aligning to the query:

278:283 binding L-methionine
295 N→A: Reduces the binding of L-methionine to undetectable levels.
295:296 binding L-methionine

3tuzC Inward facing conformations of the metni methionine abc transporter: cy5 semet soak crystal form (see paper)
39% identity, 85% coverage: 3:206/241 of query aligns to 1:204/344 of 3tuzC

query
sites
3tuzC

E

H

V

M

V

I

K

K

I

L

T

S

D

N

V

I

T

T

K

K

V

V

Y
x
F

T

H

M
x
Q

G

G

D

T

Q

R

R

T

V

I

E

Q

A

A

L

L

K

N

G

N

I

V

S

S

F

L

S

H

I

V

R

P

R

A

G

G

E

Q

F

I

V

Y

A

G

I

V

M

I

G

G

A

A

S

S

G
|
G

S

A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

M

C

L

V

G

N

C

L

L

L

D

E

V

R

Q

P

T

T

S

E

G

G

E

S

Y

V

L

L

L

V

E

D

E

G

V

Q

N

E

V

L

G

T

Q

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

E

K

I

A

R

R

N

-

R

R

K

Q

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

A

S

R

S

T

R

T

T

A

V

R

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

V

E

Y

L

A

D

H

N

V

T

P

P

A

K

A

D

E

E

R

V

R

K

E

R

R

R

A

V

H

T

T

E

S

L

L

L

A

S

R

L

V

V

G

G

L

L

A

G

G

D

R

K

E

H

G

D

H

S

Y

Y

S

P

N

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

S

E

N

P

P

A

K

I

V

I

L

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

S

P

K

A

T

T

A

T

V

R

E

S

I

I

M

L

A

E

I

L

F

L

Q

K

E

D

L

I

N

N

R

R

Q

R

-

L

G

G

I

L

T

T

I

I

I

L

M

L

V

I

T

T

H

H

E

E

P

M

E

D

V

V

binding adenosine-5'-diphosphate: F12 (≠ Y14), Q14 (≠ M16), G42 (= G44), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49)

Sites not aligning to the query:

binding selenomethionine: 274, 279, 281, 282, 283, 284, 311, 313

3tuiC Inward facing conformations of the metni methionine abc transporter: cy5 native crystal form (see paper)
39% identity, 85% coverage: 3:206/241 of query aligns to 1:204/344 of 3tuiC

query
sites
3tuiC

E

H

V

M

V

I

K

K

I

L

T

S

D

N

V

I

T

T

K

K

V

V

Y
x
F

T

H

M

Q

G

G

D

T

Q

R

R

T

V

I

E

Q

A

A

L

L

K

N

G

N

I

V

S

S

F

L

S

H

I

V

R

P

R

A

G

G

E

Q

F

I

V

Y

A

G

I

V

M

I

G

G

A

A

S

S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

M

C

L

V

G

N

C

L

L

L

D

E

V

R

Q

P

T

T

S

E

G

G

E

S

Y

V

L

L

L

V

E

D

E

G

V

Q

N

E

V

L

G

T

Q

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

E

K

I

A

R

R

N

-

R

R

K

Q

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

A

S

R

S

T

R

T

T

A

V

R

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

V

E

Y

L

A

D

H

N

V

T

P

P

A

K

A

D

E

E

R

V

R

K

E

R

R

R

A

V

H

T

T

E

S

L

L

L

A

S

R

L

V

V

G

G

L

L

A

G

G

D

R

K

E

H

G

D

H

S

Y

Y

S

P

N

S

Q

N

L

L

S

S

G

G

G

G

Q

Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

S

E

N

P

P

A

K

I

V

I

L

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

S

P

K

A

T

T

A

T

V

R

E

S

I

I

M

L

A

E

I

L

F

L

Q

K

E

D

L

I

N

N

R

R

Q

R

-

L

G

G

I

L

T

T

I

I

I

L

M

L

V

I

T

T

H

H

E

E

P

M

E

D

V

V

binding adenosine-5'-diphosphate: F12 (≠ Y14), G42 (= G44), A43 (≠ S45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49)

6cvlD Crystal structure of the escherichia coli atpgs-bound metni methionine abc transporter in complex with its metq binding protein (see paper)
39% identity, 85% coverage: 3:206/241 of query aligns to 1:204/344 of 6cvlD

query
sites
6cvlD

E

H

V

M

V

I

K

K

I

L

T

S

D

N

V

I

T

T

K

K

V

V

Y
x
F

T

H

M
x
Q

G

G

D

T

Q

R

R

T

V
x
I

E

Q

A

A

L

L

K

N

G

N

I

V

S

S

F

L

S

H

I

V

R

P

R

A

G

G

E

Q

F

I

V

Y

A

G

I

V

M

I

G

G

A

A

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

I

N

R

M

C

L

V

G

N

C

L

L

L

D

E

V

R

Q

P

T

T

S

E

G

G

E

S

Y

V

L

L

L

V

E

D

E

G

V

Q

N

E

V

L

G

T

Q

T

L

L

S

S

R

E

N

S

E

E

L

L

A

T

E

K

I

A

R

R

N

-

R

R

K

Q

L

I

G

G

F

M

V

I

F

F

Q

Q

G

H

F

F

N

N

L

L

L

L

A

S

R

S

T

R

T

T

A

V

R

F

E

G

N

N

V

V

E

A

L

L

P

P

L

L

V

E

Y

L

A

D

H

N

V

T

P

P

A

K

A

D

E

E

R

V

R

K

E

R

R

R

A

V

H

T

T

E

S

L

L

L

A

S

R

L

V

V

G

G

L

L

A

G

G

D

R

K

E

H

G

D

H

S

Y

Y

S

P

N

S

Q
x
N

L

L

S
|
S

G

G

G

G

Q
|
Q

Q

K

Q

Q

R

R

V

V

A

A

I

I

A

A

R

R

A

A

L

L

V

A

N

S

E

N

P

P

A

K

I

V

I

L

L

L

A

C

D

D

E

Q

P

A

T

T

G

S

N

A

L

L

D

D

S

P

K

A

T

T

A

T

V

R

E

S

I

I

M

L

A

E

I

L

F

L

Q

K

E

D

L

I

N

N

R

R

Q

R

-

L

G

G

I

L

T

T

I

I

I

L

M

L

V

I

T

T

H
|
H

E

E

P

M

E

D

V

V

binding phosphothiophosphoric acid-adenylate ester: F12 (≠ Y14), Q14 (≠ M16), I19 (≠ V21), S41 (= S43), G42 (= G44), A43 (≠ S45), G44 (= G46), K45 (= K47), S46 (= S48), T47 (= T49), N141 (≠ Q144), S143 (= S146), Q146 (= Q149), H200 (= H202)

8w6iD Cryo-em structure of escherichia coli str k12 ftsex complex with atp- gamma-s in peptidisc
39% identity, 83% coverage: 4:203/241 of query aligns to 1:196/219 of 8w6iD

query
sites
8w6iD

V

M

V

I

K

R

I

F

T

E

D

H

V

V

T

S

K

K

V

A

Y
|
Y

T

L

M

G

G

G

D

R

Q

Q

R

-

V

-

E

-

A

A

L

L

K

Q

G

G

I

V

S

T

F

F

S

H

I

M

R

Q

R

P

G

G

E

E

F

M

V

A

A

F

I

L

M

T

G

G

A

H

S
|
S

G
|
G

S
x
A

G
|
G

K
|
K

S
|
S

T
|
T

C

L

M

L

N

K

M

L

L

I

G

C

C

G

L

I

D

E

V

R

Q

P

T

S

S

A

G

G

E

K

Y

I

L

W

L

F

E

S

E

G

V

H

N

D

V

I

G

T

Q

R

L

L

S

K

R

N

N

R

E

E

L

V

A

P

E

F

I

L

R

R

N

-

R

R

K

Q

L

I

G

G

F

M

V

I

F

F

Q
|
Q

G

D

F

H

N

H

L

L

L

L

A

M

R

D

T

R

T

T

A

V

R

Y

E

D

N

N

V

V

E

A

L

I

P

P

L

L

V

I

Y

I

A

A

H

G

V

A

P

S

A

G

A

D

E

D

R

I

R

R

E

R

R

R

A

V

H

S

T

A

S

A

L

L

A

D

R

K

V

V

G

G

L

L

A

L

G

D

R
x
K

E

A

G

K

H

N

Y

F

S

P

N

I

Q
|
Q

L

L

S
|
S

G
|
G

G
|
G

Q
x
E

Q

Q

Q

Q

R

R

V

V

A

G

I

I

A

A

R

R

A

A

L

V

V

V

N

N

E

K

P

P

A

A

I

V

I

L

L

L

A

A

D

D

E

E

P

P

T

T

G

G

N

N

L

L

D

D

S

D

K

A

T

L

A

S

V

E

E

G

I

I

M

L

A

R

I

L

F

F

Q

E

E

E

L

F

N

N

R

R

Q

V

G

G

I

V

T

T

I

V

I

L

M

M

V

A

T

T

H
|
H

E

D

binding phosphothiophosphoric acid-adenylate ester: Y11 (= Y14), S37 (= S43), G38 (= G44), A39 (≠ S45), G40 (= G46), K41 (= K47), S42 (= S48), T43 (= T49), Q86 (= Q93), K130 (≠ R137), Q137 (= Q144), S139 (= S146), G140 (= G147), G141 (= G148), E142 (≠ Q149), H195 (= H202)

Query Sequence

>WP_011938055.1 NCBI__GCF_000016745.1:WP_011938055.1
MDEVVKITDVTKVYTMGDQRVEALKGISFSIRRGEFVAIMGASGSGKSTCMNMLGCLDVQ
TSGEYLLEEVNVGQLSRNELAEIRNRKLGFVFQGFNLLARTTARENVELPLVYAHVPAAE
RRERAHTSLARVGLAGREGHYSNQLSGGQQQRVAIARALVNEPAIILADEPTGNLDSKTA
VEIMAIFQELNRQGITIIMVTHEPEVAAFTGRHIIFRDGVIISDTRNATPTVAAPAEEQS
P

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory