SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011938952.1 NCBI__GCF_000016745.1:WP_011938952.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
54% identity, 99% coverage: 1:396/399 of query aligns to 4:397/654 of P36204

query
sites
P36204

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R36 (= R38) binding substrate
R118 (= R120) binding substrate
R151 (= R153) binding substrate

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
54% identity, 98% coverage: 1:390/399 of query aligns to 3:390/398 of 1vpeA

query
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1vpeA

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active site: R35 (= R38), K196 (= K200), G353 (= G353), G376 (= G376)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G198), A195 (= A199), K196 (= K200), K200 (= K204), G218 (= G222), A219 (≠ G223), N316 (= N319), P318 (= P321), G320 (= G323), V321 (= V324), E323 (= E326), G352 (= G352), G353 (= G353), D354 (= D354), S355 (≠ T355)

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
53% identity, 96% coverage: 11:395/399 of query aligns to 10:393/394 of 1phpA

query
sites
1phpA

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active site: R36 (= R38), K197 (= K200), G351 (= G353), G374 (= G376)
binding adenosine-5'-diphosphate: G195 (= G198), K201 (= K204), G219 (= G222), G220 (= G223), L237 (= L240), N316 (= N319), P318 (= P321), G320 (= G323), V321 (= V324), E323 (= E326), G350 (= G352), D352 (= D354), S353 (≠ T355)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
53% identity, 96% coverage: 11:395/399 of query aligns to 10:393/394 of P18912

query
sites
P18912

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E

Y

L

S

R

M

L

A

D

P

A

A

V

A

A

K

K

R

R

L

L

S

G

R

E

L

L

G

E

K

R

E

P

V

V

K

A

L

K

A

T

P

N

D

E

C

A

I

V

G

G

D

D

G

E

V

V

S

K

K

A

I

A

I

V

D

D

S

R

M

L

Q

N

P

E

G

G

D

D

V

V

V

L

M

L

L

L

E

E

N

N

L

V

R

R

F

F

Y

Y

P

P

G

G

E

E

K

K

N

N

D

D

D

P

D

E

F

L

A

A

K

K

A

A

L

F

A

A

D

E

H

L

C

A

D

D

I

L

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

A

S

A

H

H

R

R

A

A

H

H

A

A

S

S

V

T

E

E

A

G

I

I

T

A

K

H

F

Y

F

L

P

P

I

A

V

V

A

A

A

-

G

G

F

F

L

L

M

M

K

E

N

K

E

E

M

L

S

E

Y

V

F

L

E

G

K

K

S

A

M

L

K

S

N

N

P

P

I

D

R

R

P

P

L

F

V

T

A

A

I

I

L

I

G

G

A

A

K

K

V

V

S

K

G

D

K
|
K

L

I

E

G

V

V

L

I

E

D

N

N

L

L

C

L

N

E

K

K

V

V

D

D

K

N

V

L

I

I

G

G

M

L

A

A

F

Y

T

T

F

F

L

V

K

K

A

A

L

L

G

G

Y

H

N

D

V

V

G

G

K

K

S

S

L

L

V

L

E

E

N

D

L

K

L

I

E

E

T

L

A

A

L

K

N

S

T

F

Y

M

N

E

K

K

A

A

R

K

E

E

K

K

G

G

I

V

K

R

F

F

Y

Y

L

M

P

P

V

V

D

D

C

V

V

V

A

A

D

D

Q

R

F

F

N

A

P

N

A

D

A

A

E

N

T

T

K

K

V

V

T

V

T

P

I

I

Q

D

E

A

I

I

P

P

E

A

G

D

W

W

M

S

A

A

L

L

D

D

I

I

G

G

P

P

A

K

T

T

V

R

T

E

L

L

F

Y

S

R

T

D

A

V

L

I

Q

R

N

E

A

S

K

K

T

L

I

V

V

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

M

M

D

D

A

A

F

F

S

A

R

H

G

G

T

T

-

K

-

A

-

I

F

A

A

E

M

A

V

L

S

A

A

E

V

A

A

L

N

D

S

T

Y

Y

A

-

L

-

T

S

I

V

V

I

G

G

G
|
G

D
|
D

T
x
S

D

A

V

A

A

A

V

V

H

E

R

K

A

F

G

G

E

L

Y

A

A

D

K

K

I

M

S

D

Y

H

I

I

S

S

T

T

G

G

A

A

F

S

L

L

E

E

L

F

L

M

E

E

G

G

K

K

K

Q

L

L

P

P

G

G

I

V

K

V

V

A

L

L

E

E

D

D

K201 (= K204) binding ATP
N316 (= N319) binding ATP
E323 (= E326) binding ATP
GGDS 350:353 (≠ GGDT 352:355) binding ATP

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
53% identity, 96% coverage: 11:395/399 of query aligns to 10:393/394 of P40924

query
sites
P40924

D

D

L

V

R

K

E

G

K

K

K

V

I

V

F

F

M

C

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

K

E

D

H

G

Q

E

N

-

I

V

T

T

E

D

D

D

T

T

R

R

I

I

R

R

A

A

V

A

L

L

P

P

T

T

I

I

N

K

Y

H

A

L

L

A

D

D

Y

Q

N

G

A

A

K

K

V

V

V

L

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

E

E

R

V

N

V

A

E

K

E

Y

L

S

R

M

L

A

T

P

P

A

V

A

A

K

A

R

R

L

L

S

G

R

E

L

L

G

G

K

K

E

E

V

V

K

K

L

K

A

A

P

D

D

E

C

A

I

Y

G

G

D

D

G

A

V

V

S

K

K

A

I

Q

I

I

D

S

S

E

M

M

Q

K

P

D

G

G

D

D

V

V

V

L

M

V

L

L

E

E

N

N

L

V

R

R

F

F

Y

Y

P

P

G

G

E

E

K

K

N

N

D

D

D

P

D

E

F

L

A

A

K

K

A

A

L

F

A

A

D

E

H

L

C

A

D

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

A

S

A

H

H

R

R

A

A

H

H

A

A

S

S

V

T

E

A

A

G

I

I

T

A

K

E

F

H

F

L

P

P

I

A

V

V

A

A

A

-

G

G

F

F

L

L

M

M

K

E

N

K

E

E

M

L

S

D

Y

V

F

L

E

G

K

K

S

A

M

V

K
x
S

N

N

P

P

I

D

R

R

P

P

L

F

V

T

A

A

I

I

L

I

G

G

A

A

K

K

V

V

S

K

G

D

K

K

L

I

E

G

V

V

L

I

E

E

N

S

L

L

C

L

N

D

K

K

V

V

D

D

K

N

V

L

I

I

G

G

M

L

A

A

F

Y

T

T

F

F

L

V

K

K

A

A

L

L

G

G

Y

Y

N

E

V

V

G

G

K

K

S

S

L

L

V

L

E

E

N

D

L

K

L

I

E

E

T

L

A

A

L

K

N

S

T

F

Y

M

N

D

K

R

A

A

R

K

E

E

K

K

G

G

I

V

K

N

F

F

Y

Y

L

M

P

P

V

E

D

D

C

V

V

L

V

V

A

A

D

D

Q

D

F

F

N

S

P

N

A

D

A

A

E

N

T

V

K

K

V

I

T

V

T

P

I

I

Q

S

E

E

I

I

P

P

E

S

G

D

W

L

M

E

A

A

L

I

D

D

I

I

G

G

P

T

A

K

T
|
T

V

R

T

E

L

T

F

Y

S

A

T

D

A

V

L

I

Q

K

N

N

A

S

K

K

T

L

I

V

V

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

F

F

E

E

M

I

D

D

A

L

F

F

S

A

R

Q

G

G

T

T

F

K

A

A

M

V

V

A

S

E

A

A

V

L

A

A

N

E

S

A

Y

K

-

D

A

T

L

Y

T

S

I

V

V

I

G

G

D

D

T

S

D

A

V

A

A

A

V

V

H

E

R

K

A

F

G

G

E

L

Y

A

A

D

K

K

I

M

S

S

Y

H

I

I

S

S

T

T

G

G

A

A

F

S

L

L

E

E

L

F

L

M

E

E

G

G

K

K

K

E

L

L

P

P

G

G

I

V

K

A

V

A

L

L

E

N

D

D

S183 (≠ K186) modified: Phosphoserine
T299 (= T302) modified: Phosphothreonine

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
48% identity, 96% coverage: 11:395/399 of query aligns to 13:414/416 of P00560

query
sites
P00560

D

D

L

L

R

K

E

D

K

K

K

R

I

V

F

F

M

I

R
|
R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

-

H

G

Q

K

N

K

I

I

T

T

E

S

D

N

T

Q

R
|
R

I

I

R

V

A

A

V

A

L

L

P

P

T

T

I

I

N

K

Y

Y

A

V

L

L

D

E

Y

H

N

H

A

P

K

R

-

Y

V

V

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

E

E

R

R

N

N

A

E

K

K

Y

Y

S

S

M

L

A

A

P

P

A

V

A

A

K

K

R

E

L

L

S

Q

R

S

L

L

G

G

K

K

E

D

V

V

K

T

L

F

A

L

P

N

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

A

I

A

I

V

D

K

S

A

M

S

Q

A

P

P

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R
|
R

F

Y

Y

H

P

I

G

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

K

K

N

E

D

D

-

V

D

Q

D

K

F

F

A

R

K

H

A

E

L

L

A

S

D

S

H

L

C

A

D

D

I

V

Y

Y

V

I

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R
|
R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

F

T

D

K

-

F

-

F

L

P

P

I

Q

V

R

A

A

G

G

F

F

L

L

M

L

K

E

N

K

E

E

M

L

S

K

Y

Y

F

F

E

G

K

K

S

A

M

L

K

E

N

N

P

P

I

T

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

A

A

K

K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

N

N

L

L

C

L

N

D

K

K

V

V

D

D

K

S

V

I

I

I

G

G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

A

V

L

L

-

E

G

N

Y

T

N

E

V

I

G

G

K

D

S

S

L

I

V

F

E

D

E

K

N

A

L

G

L

A

E

E

T

I

A

V

L

P

N

K

T

L

Y

M

N

E

K

K

A

A

R

K

E

A

K

K

G

G

I

V

K

E

F

V

Y

V

L

L

P

P

V

V

D

D

C

F

V

I

A

A

D

D

Q

A

F

F

N

S

P

A

A

D

A

A

E

N

T

T

K

K

V

T

T

V

T

T

I

D

Q

K

E

E

-

G

I

I

P

P

E

A

G

G

W

W

M

Q

A

G

L

L

D

D

I

N

G

G

P

P

A

E

T

S

V

R

T

K

L

L

F

F

S

A

T

A

A

T

L

V

Q

A

N

K

A

A

K

K

T

T

I

I

V

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

F

F

E

E

M

F

D

E

A

K

F

F

S

A

R

A

G

G

T

T

F

K

A

A

M

L

V

L

S

D

A

E

V

V

A

V

N

K

S

S

Y

S

A

A

-

A

-

G

-

N

L

T

T

V

I

I

V

I

G

G

D

D

T

T

D

A

V

T

A

V

V

A

H

K

R

K

A

Y

G

G

E

V

Y

T

A

D

K

K

I

I

S

S

Y

H

I

V

S

S

T

T

G

G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

E

L

L

P

P

G

G

I

V

K

A

V

F

L

L

E

S

D

E

R22 (= R20) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R38) binding substrate
R122 (= R120) binding substrate
R169 (= R153) binding substrate

1qpgA 3-phosphoglycerate kinase, mutation r65q (see paper)
48% identity, 96% coverage: 11:395/399 of query aligns to 12:413/415 of 1qpgA

query
sites
1qpgA

D

D

L

L

R

K

E

D

K

K

K

R

I

V

F

F

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

-

H

G

Q

K

N

K

I

I

T

T

E

S

D

N

T

Q

R
|
R

I

I

R

V

A

A

V

A

L

L

P

P

T

T

I

I

N

K

Y

Y

A

V

L

L

D

E

Y

H

N

H

A

P

K

R

-

Y

V

V

L

L

A

A

S

S

H

H

L

L

G

G

R

Q

P

P

K

N

G

G

E

E

R

R

N

N

A

E

K

K

Y

Y

S

S

M

L

A

A

P

P

A

V

A

A

K

K

R

E

L

L

S

Q

R

S

L

L

G

G

K

K

E

D

V

V

K

T

L

F

A

L

P

N

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

A

I

A

I

V

D

K

S

A

M

S

Q

A

P

P

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

Y

Y

H

P

I

G

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

K

K

N

E

D

D

-

V

D

Q

D

K

F

F

A

R

K

H

A

E

L

L

A

S

D

S

H

L

C

A

D

D

I

V

Y

Y

V

I

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

F

T

D

K

-

F

-

F

L

P

P

I

Q

V

R

A

A

G

G

F

F

L

L

M

L

K

E

N

K

E

E

M

L

S

K

Y

Y

F

F

E

G

K

K

S

A

M

L

K

E

N

N

P

P

I

T

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

A

A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

N

N

L

L

C

L

N

D

K

K

V

V

D

D

K

S

V

I

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

A

V

L

L

-

E

G

N

Y

T

N

E

V

I

G

G

K

D

S

S

L

I

V

F

E

D

E

K

N

A

L

G

L

A

E

E

T

I

A

V

L

P

N

K

T

L

Y

M

N

E

K

K

A

A

R

K

E

A

K

K

G

G

I

V

K

E

F

V

Y

V

L

L

P

P

V

V

D

D

C

F

V

I

A

A

D

D

Q

A

F

F

N

S

P

A

A

D

A

A

E

N

T

T

K

K

V

T

T

V

T

T

I

D

Q

K

E

E

-

G

I

I

P

P

E

A

G

G

W

W

M

Q

A

G

L

L

D

D

I

N

G

G

P

P

A

E

T

S

V

R

T

K

L

L

F

F

S

A

T

A

A

T

L

V

Q

A

N

K

A

A

K

K

T

T

I

I

V

V

W

W

N
|
N

G

G

P
|
P

M

P

G
|
G

V
|
V

F
|
F

E
|
E

M

F

D

E

A

K

F

F

S

A

R

A

G

G

T

T

F

K

A

A

M

L

V

L

S

D

A

E

V

V

A

V

N

K

S

S

Y

S

A

A

-

A

-

G

-

N

L

T

T

V

I

I

V

I

G

G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

V

V

A

H

K

R

K

A

Y

G

G

E

V

Y

T

A

D

K

K

I

I

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

E

L

L

P

P

G

G

I

V

K

A

V

F

L

L

E

S

D

E

active site: R38 (= R38), K213 (= K200), G371 (= G353), G394 (= G376)
binding magnesium-5'-adenyly-imido-triphosphate: G235 (= G222), G236 (= G223), N334 (= N319), P336 (= P321), G338 (= G323), V339 (= V324), F340 (= F325), E341 (= E326), G370 (= G352), G371 (= G353), D372 (= D354), T373 (= T355)

3pgkA The structure of yeast phosphoglycerate kinase at 0.25 nm resolution (see paper)
47% identity, 96% coverage: 11:395/399 of query aligns to 12:413/415 of 3pgkA

query
sites
3pgkA

D

D

L

L

R

K

E

D

K

K

K

R

I

V

F

F

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

-

H

G

Q

K

N

K

I

I

T

T

E

S

D

N

T

Q

R
|
R

I

I

R

V

A

A

V

A

L

L

P

P

T

T

I

I

N

K

Y

Y

A

V

L

L

D

E

Y

H

N

H

A

P

K

R

-

Y

V

V

L

L

A

A

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

E

E

R

R

N

N

A

E

K

K

Y

Y

S

S

M

L

A

A

P

P

A

V

A

A

K

K

R

E

L

L

S

Q

R

S

L

L

G

G

K

K

E

D

V

V

K

T

L

F

A

L

P

N

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

A

I

A

I

V

D

K

S

A

M

S

Q

A

P

P

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

Y

Y

H

P

I

G

E

E

E

-

G

-

S

-

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

K

K

N

E

D

D

-

V

D

Q

D

K

F

F

A

R

K

H

A

E

L

L

A

S

D

S

H

L

C

A

D

D

I

V

Y

Y

V

I

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

F

T

D

K

-

F

-

F

L

P

P

I

Q

V

R

A

A

G

G

F

F

L

L

M

L

K

E

N

K

E

E

M

L

S

K

Y

Y

F

F

E

G

K

K

S

A

M

L

K

E

N

N

P

P

I

T

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

N

N

L

L

C

L

N

D

K

K

V

V

D

D

K

S

V

I

I

I

G

G

M

M

A

A

F

F

T

T

F

F

L

K

K

K

A

V

L

L

-

E

G

N

Y

T

N

E

V

I

G

G

K

D

S

S

L

I

V

F

E

D

E

K

N

A

L

V

L

G

E

P

T

E

A

I

L

A

N

K

T

L

Y

M

N

E

K

K

A

A

R

K

E

A

K

K

G

G

I

V

K

E

F

V

Y

V

L

L

P

P

V

V

D

D

C

F

V

I

A

A

D

D

Q

A

F
|
F

N

S

P

A

A

S

A

A

E

N

T

T

K

K

V

T

T

V

T

T

I

D

Q

K

E

E

-

G

I

I

P

P

E

A

G

G

W

W

M

Q

A

G

L
|
L

D

D

I

N

G

G

P

P

A

E

T

S

V

R

T

K

L

L

F

F

S

A

T

A

A

T

L

V

Q

A

N

K

A

A

K

T

T

V

I

I

V

L

W

W

N
|
N

G
|
G

P
|
P

M

P

G
|
G

V
|
V

F

F

E

E

M

F

D

E

A

K

F

F

S

A

R

A

G

G

T

T

F

K

A

A

M

L

V

L

S

D

A

E

V

V

A

V

N

K

S

S

Y

S

A

A

-

A

-

G

-

N

L

T

T

V

I

I

V

I

G

G

G
|
G

D
|
D

T

T

D

A

V

T

A

V

V

A

H

K

R

K

A

Y

G

G

E

V

Y

T

A

D

K

K

I

I

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

E

L

L

P

P

G

G

I

V

K

A

V

F

L

L

E

S

D

E

active site: R38 (= R38), K213 (= K200), G371 (= G353), G394 (= G376)
binding adenosine-5'-triphosphate: G211 (= G198), A212 (= A199), K213 (= K200), F289 (= F275), L311 (= L296), N334 (= N319), G335 (= G320), P336 (= P321), G338 (= G323), V339 (= V324), D372 (= D354)

4feyA An x-ray structure of a putative phosphogylcerate kinase with bound adp from francisella tularensis subsp. Tularensis schu s4
46% identity, 96% coverage: 11:395/399 of query aligns to 10:388/392 of 4feyA

query
sites
4feyA

D

D

L

L

R

K

E

D

K

K

I

V

F

L

M

V

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

V

D

K

E

D

H

G

Q

K

N

-

I

V

T

T

E

S

D

K

T

V

R
|
R

I

I

R

E

A

A

V

A

L

I

P

P

T

T

I

I

N

Q

Y

Y

A

I

L

L

D

D

Y

Q

N

G

A

G

K

A

V

V

V

I

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

-

T

K

E

G

G

E

E

R

Y

N

D

A

S

K

Q

Y

F

S

S

M

L

A

E

P

P

A

V

A

A

K

K

R

A

L

L

S

S

R

E

L

I

G

N

K

K

E

P

V

V

K

K

L

F

A

A

P

K

D

D

C

W

I

L

G

-

D

D

G

G

V

V

S

D

K

-

I

-

D

-

S

-

M

V

Q

K

P

A

G

G

D

E

V

I

V

V

M

M

L

C

E

E

N

N

L

V

R

R

F

F

Y

N

P

S

G

G

E

E

E

K

K

K

N

S

D

T

D

D

F

L

A

S

K

K

A

K

L

I

A

A

D

S

H

L

C

G

D

D

I

V

Y

F

V

V

N

M

D

D

A

A

F

F

A

A

V

T

S

A

H

H

R

R

A

A

H

Q

A

A

S

S

V

T

E

Y

A

G

I

V

T

A

K

K

F

Y

F

I

P

P

I

V

V

A

A

C

A

A

G

G

F

I

L

L

M

L

K

T

N

N

E

E

M

I

S

Q

Y

A

F

L

E

E

K

K

S

A

M

L

K

K

N

S

P

P

I

K

R

K

P

P

L

M

V

A

A

A

I

I

L

V

G

G

G
|
G

A
x
S

K
|
K

V

V

S

S

G

T

K
|
K

L

L

E

S

V

V

L

L

E

N

N

N

L

L

C

L

N

D

K

K

V

V

D

E

K

I

V

L

I

I

I

V

G

G

G
|
G

G

G

M

I

A

A

F

N

T

T

F

F

L

I

K

K

A

A

L

E

G

G

Y

F

N

D

V

V

G

G

K

N

S

S

L

L

V

Y

E

E

E

Q

N

D

L

L

L

V

E

A

T

E

A

A

L

T

N

E

T

I

Y

L

N

A

K

K

A

A

R

K

E

A

K

L

G

G

I

V

K

N

F

I

Y

P

L

V

P

P

V

V

D

D

C

V

V

R

V

V

A

A

D

K

Q

E

F

F

N

S

P

E

A

N

A

A

E

Q

T

A

K

I

V

I

T

K

I

V

Q

S

E

D

I

V

P

V

E

A

G

D

W

E

M

M

A

I

L

L

D

D

I

I

G

G

P

P

A

E

T

S

V

Q

T

K

L

I

F

I

S

A

T

E

A

L

L

L

Q

K

N

S

A

A

K

N

T

T

I

I

V

L

W

W

N

N

G

G

P

P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

F

D

D

A

N

F

F

S

A

R

E

G

G

T

T

F

K

A

A

M

L

V

S

S

L

A

A

V

I

A

A

N

Q

S

S

Y

H

A

A

L

F

T

S

I

V

V

A

G

G

G
|
G

D
|
D

T

T

D

I

V

A

A

A

V

I

H

E

R

K

A

F

G

G

E

I

Y

K

A

D

K

Q

I

V

S

S

Y

Y

I

I

S

S

T

T

G

A

G

G

G
|
G

A

A

F

F

L

L

E

E

L

F

L

L

E

E

G

G

K

K

L

L

P

P

G

A

I

I

K

E

V

I

L

L

E

K

D

E

active site: R36 (= R38), K193 (= K200), G346 (= G353), G369 (= G376)
binding adenosine-5'-diphosphate: G191 (= G198), S192 (≠ A199), K197 (= K204), G215 (= G222), G316 (= G323), V317 (= V324), E319 (= E326), D347 (= D354)

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
46% identity, 97% coverage: 11:396/399 of query aligns to 9:415/415 of 16pkA

query
sites
16pkA

D

D

L

L

R

K

E

G

K

K

I

V

F

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

V

D

-

E

K

H

N

Q

G

N

K

I

I

T

T

E

N

D

D

T

Y

R
|
R

I

I

R

R

A

S

V

A

L

L

P

P

T

T

I

L

N

K

Y

K

A

V

L

L

D

T

Y

E

N

G

A

G

K

S

V

C

V

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

E

I

R

P

N

M

A

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

V

-

P

-

G

-

F

-

Q

K

K

Y

A

S

T

M

L

A

K

P

P

A

V

A

A

K

K

R

R

L

L

S

S

R

E

L

L

G

L

K

R

E

P

V

V

K

T

L

F

A

A

P

P

D

D

C

C

I

L

G

N

D

-

G

-

V

A

S

A

K

D

I

V

D

S

S

K

M

M

Q

S

P

P

G

G

D

D

V

V

M

L

L

L

E

E

N

N

L

V

R

R

F

F

Y

Y

P

K

G

E

E

E

-

G

-

S

-

K

E

A

K

K

N

D

D

R

D

E

D

A

F

M

A

A

K

K

A

I

L

L

A

A

D

S

H

Y

C

G

D

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

D

H

S

A

A

S

T

V

M

E

T

A

G

I

I

T

P

K

K

F

I

F

L

P

G

I

N

V

G

A

A

G

G

F

Y

L

L

M

M

K

E

N

K

E

E

M

I

S

S

Y

Y

F

F

E

A

K

K

S

V

M

L

K

G

N

N

P

P

I

P

R

R

P

P

L

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

G

D

K
|
K

L

I

E

Q

V

L

L

L

E

D

N

N

L

M

C

L

N

Q

K

R

V

I

D

D

K

Y

V

L

I

L

I

I

G

G

G
x
A

M

M

A

A

F
x
Y

T

T

F

F

L

L

K

K

A

A

L

Q

G

G

Y

Y

N

S

V

I

G

G

K

K

S

S

L

K

V

C

E

E

N

S

L

K

L

L

E

E

T

F

A

A

L

R

N

S

T

L

Y

L

N

K

K

K

A

A

R

E

E

D

K

R

G

K

I

V

K

Q

F

V

Y

I

L

L

P

P

V

I

D

D

C

H

V

V

V

C

A

H

D

T

Q

E

F

F

N

K

P

A

A

V

A

D

E

S

T

P

K

L

V

I

T

T

T

E

I

D

Q

Q

E

N

I

I

P

P

E

E

G

G

W

H

M

M

A

A

L
|
L

D

D

I

I

G

G

P

P

A

K

T

T

V

I

T

E

L

K

F

Y

S

V

T

Q

A

T

L

I

Q

G

N

K

A

C

K

K

T

S

I

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

D

V

A

P

F

Y

S

S

R

K

G

G

T

T

F

F

A

A

M

I

V

A

S

K

A

A

V

M

A

G

N

R

S

G

Y

T

A

H

-

E

-

H

-

G

-

L

L

M

T

S

I

I

V

I

G

G

G
|
G

D

D

T

S

D

A

V

S

A

A

V

A

H

E

R

L

A

S

G

G

E

E

Y

A

A

K

K

R

I

M

S

S

Y

H

I

V

S

S

T

T

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

T

L

L

P

P

G

G

I

V

K

T

V

V

L

L

E

D

D

D

N

K

active site: R35 (= R38), K215 (= K200), G372 (= G353), G395 (= G376)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G198), A214 (= A199), K219 (= K204), A238 (≠ G223), Y241 (≠ F226), L311 (= L296), P336 (= P321), G338 (= G323), V339 (= V324), E341 (= E326), G393 (= G374), G394 (= G375), G395 (= G376)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
46% identity, 97% coverage: 11:396/399 of query aligns to 9:415/415 of 13pkA

query
sites
13pkA

D

D

L

L

R

K

E

G

K

K

I

V

F

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

V

D

-

E

K

H

N

Q

G

N

K

I

I

T

T

E

N

D

D

T

Y

R
|
R

I

I

R

R

A

S

V

A

L

L

P

P

T

T

I

L

N

K

Y

K

A

V

L

L

D

T

Y

E

N

G

A

G

K

S

V

C

V

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

K

G

G

E

I

R

P

N

M

A

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

V

-

P

-

G

-

F

-

Q

K

K

Y

A

S

T

M

L

A

K

P

P

A

V

A

A

K

K

R

R

L

L

S

S

R

E

L

L

G

L

K

R

E

P

V

V

K

T

L

F

A

A

P

P

D

D

C

C

I

L

G

N

D

-

G

-

V

A

S

A

K

D

I

V

D

S

S

K

M

M

Q

S

P

P

G

G

D

D

V

V

M

L

L

L

E

E

N

N

L

V

R

R

F

F

Y

Y

P

K

G

E

E

E

-

G

-

S

-

K

E

A

K

K

N

D

D

R

D

E

D

A

F

M

A

A

K

K

A

I

L

L

A

A

D

S

H

Y

C

G

D

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

D

H

S

A

A

S

T

V

M

E

T

A

G

I

I

T

P

K

K

F

I

F

L

P

G

I

N

V

G

A

A

G

G

F

Y

L

L

M

M

K

E

N

K

E

E

M

I

S

S

Y

Y

F

F

E

A

K

K

S

V

M

L

K

G

N

N

P

P

I

P

R

R

P

P

L

L

V

V

A

A

I

I

L

V

G

G

G
|
G

A
|
A

K
|
K

V

V

S

S

G

D

K
|
K

L

I

E

Q

V

L

L

L

E

D

N

N

L

M

C

L

N

Q

K

R

V

I

D

D

K

Y

V

L

I

L

I

I

G

G

G

A

M

M

A

A

F

Y

T

T

F

F

L

L

K

K

A

A

L

Q

G

G

Y

Y

N

S

V

I

G

G

K

K

S

S

L

K

V

C

E

E

N

S

L

K

L

L

E

E

T

F

A

A

L

R

N

S

T

L

Y

L

N

K

K

K

A

A

R

E

E

D

K

R

G

K

I

V

K

Q

F

V

Y

I

L

L

P

P

V

I

D

D

C

H

V

V

V

C

A

H

D

T

Q

E

F

F

N

K

P

A

A

V

A

D

E

S

T

P

K

L

V

I

T

T

T

E

I

D

Q

Q

E

N

I

I

P

P

E

E

G

G

W

H

M

M

A

A

L
|
L

D

D

I

I

G

G

P

P

A

K

T

T

V

I

T

E

L

K

F

Y

S

V

T

Q

A

T

L

I

Q

G

N

K

A

C

K

K

T

S

I

A

V

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

M

M

D

V

A

P

F

Y

S

S

R

K

G

G

T

T

F

F

A

A

M

I

V

A

S

K

A

A

V

M

A

G

N

R

S

G

Y

T

A

H

-

E

-

H

-

G

-

L

L

M

T

S

I

I

V

I

G

G

G
|
G

D
|
D

T
x
S

D

A

V

S

A

A

V

A

H

E

R

L

A

S

G

G

E

E

Y

A

A

K

K

R

I

M

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

T

L

L

P

P

G

G

I

V

K

T

V

V

L

L

E

D

D

D

N

K

active site: R35 (= R38), K215 (= K200), G372 (= G353), G395 (= G376)
binding adenosine-5'-diphosphate: G213 (= G198), A214 (= A199), K219 (= K204), L311 (= L296), P336 (= P321), G338 (= G323), V339 (= V324), E341 (= E326), G371 (= G352), D373 (= D354), S374 (≠ T355)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
45% identity, 97% coverage: 11:396/399 of query aligns to 13:419/440 of P07378

query
sites
P07378

D

D

L

L

R

K

E

G

K

K

I

V

F

L

M

I

R

R

V

V

D
|
D

F
|
F

N
|
N

V

V

P

P

L

V

D

-

E

K

H

N

Q

G

N

K

I

I

T

T

E

N

D

D

T

Y

R
|
R

I

I

R

R

A

S

V

A

L

L

P

P

T

T

I

L

N

K

Y

K

A

V

L

L

D

T

Y

E

N

G

A

G

K

S

V

C

V

V

L

L

A

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

P

P

K

K

G

G

E

I

R

P

N

M

A

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

V

-

P

-

G

-

F

-

Q

K

K

Y

A

S

T

M

L

A

K

P

P

A

V

A

A

K

K

R

A

L

L

S

S

R

E

L

L

G

L

K

R

E

P

V

V

K

T

L

F

A

A

P

P

D

D

C

C

I

L

G

N

D

-

G

-

V

A

S

A

K

D

I

V

D

S

S

K

M

M

Q

S

P

P

G

G

D

D

V

V

M

L

L

L

E

E

N

N

L

V

R
|
R

F

F

Y

Y

P

K

G

E

E

E

-

G

-

S

-

K

E

A

K

K

N

D

D

R

D

E

D

A

F

M

A

A

K

K

A

I

L

L

A

A

D

S

H

Y

C

G

D

D

I

V

Y

Y

V

I

N

S

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R
|
R

A

D

H

S

A

A

S

T

V

M

E

T

A

G

I

I

T

P

K

K

F

I

F

L

P

G

I

N

V

G

A

A

G

G

F

Y

L

L

M

M

K

E

N

K

E

E

M

I

S

S

Y

Y

F

F

E

A

K

K

S

V

M

L

K

G

N

N

P

P

I

P

R

R

P

P

L

L

V

V

A

A

I

I

L

V

G

G

A

A

K

K

V

V

S

S

G

D

K
|
K

L

I

E

Q

V

L

L

L

E

D

N

N

L

M

C

L

N

Q

K

R

V

I

D

D

K

Y

V

L

I

L

I

I

G

G

G

A

M

M

A

A

F

Y

T

T

F

F

L

L

K

K

A

A

L

Q

G

G

Y

Y

N

S

V

I

G

G

K

K

S

S

L

K

V

C

E

E

N

S

L

K

L

L

E

E

T

F

A

A

L

R

N

S

T

L

Y

L

N

K

K

K

A

A

R

E

E

D

K

R

G

K

I

V

K

Q

F

V

Y

I

L

L

P

P

V

I

D

D

C

H

V

V

V

C

A

H

D

T

Q

E

F

F

N

K

P

A

A

V

A

D

E

S

T

P

K

L

V

I

T

T

T

E

I

D

Q

Q

E

N

I

I

P

P

E

E

G

G

W

H

M

M

A

A

L

L

D

D

I

I

G

G

P

P

A

K

T

T

V

I

T

E

L

K

F

Y

S

V

T

Q

A

T

L

I

Q

G

N

K

A

C

K

K

T

S

I

A

V

I

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

M

M

D

V

A

P

F

Y

S

S

R

K

G

G

T

T

F

F

A

A

M

I

V

A

S

K

A

A

V

M

A

G

N

R

S

G

Y

T

A

H

-

E

-

H

-

G

-

L

L

M

T

S

I

I

V

I

G

G

G
|
G

D
|
D

T
x
S

D

A

V

S

A

A

V

A

H

E

R

L

A

S

G

G

E

E

Y

A

A

K

K

R

I

M

S

S

Y

H

I

V

S

S

T

T

G

G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

T

L

L

P

P

G

G

I

V

K

T

V

V

L

L

E

D

D

E

N

K

DFN 24:26 (= DFN 22:24) binding substrate
R39 (= R38) binding substrate
HLGR 62:65 (= HLGR 61:64) binding substrate
R135 (= R120) binding substrate
R172 (= R153) binding substrate
K223 (= K204) binding ATP
N338 (= N319) binding ATP
E345 (= E326) binding ATP
GGDS 375:378 (≠ GGDT 352:355) binding ATP

O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
48% identity, 96% coverage: 11:393/399 of query aligns to 13:411/414 of O60101

query
sites
O60101

D

D

L

L

R

K

E

G

K

K

K

N

I

V

F

L

M

I

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

L

D

D

E

G

H

D

Q

R

N

-

I

I

T

T

E

N

D

N

T

A

R

R

I

I

R

V

A

G

V

A

L

L

P

P

T

T

I

I

N

K

Y

Y

A

A

L

L

D

E

Y

Q

N

Q

A

P

K

K

-

A

V

V

V

I

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

N

G

G

E

A

R

R

N

V

A

A

K

K

Y
|
Y

S
|
S

M

L

A

K

P

P

A

V

A

A

K

A

R

E

L

L

S

S

R

K

L

L

G

G

K

K

E

P

V

V

K

K

L

F

A

L

P

D

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

K

S

E

M

A

Q

K

P

G

G

G

D

E

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

Y

H

P

I

G

E

E

E

-

G

-

S

-

A

-

K

-

V

-

D

-

G

-

K

-

V

K

K

N

A

D

D

-

A

-
x
S

-

A

-

V

D

E

D

A

F

F

A

R

K

K

A

S

L

L

A

T

D

S

H

L

C

G

D

D

I

I

Y

F

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A
x
S

S
|
S

V

M

E

V

A

G

I

V

T

D

K

-

F

-

F

L

P

P

I

R

V

V

A
x
S

A

-

G

G

F

F

L

L

M

M

K

K

N

K

E

E

M

L

S

D

Y

Y

F

F

E

S

K

K

S

A

M

L

K

E

N

N

P

P

I

A

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

A

A

K

K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

D

N

N

L

L

C

L

N

D

K

K

V

V

D

N

K

R

V

L

I

I

G

C

G

G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

G

Y

M

N

K

V

I

G

G

K

D

S
|
S

L

L

V

F

E

D

E

E

N

A

L

G

L
x
S

E

K

T

N

A

V

L

E

N

S

T

M

Y

M

N

A

K

K

A

A

R

K

E

K

K

N

G

N

I

V

K

E

F

V

Y

F

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

Q

K

F

F

N

D

P

K

A

D

A

A

E

K

T

V

K

G

V

S

T

A

T
|
T

I

A

Q

E

E

E

-

G

I

I

P

P

E

D

G

G

W

W

M

M

A

G

L

L

D

D

I

C

G

G

P

P

A

K

T

S

V

S

T

A

L

K

F

F

S

A

T

E

A

V

L

I

Q

T

N

T

A
x
S

K

K

T

T

I

I

V

V

W

W

N

N

G

G

P

P

M

A

G

G

V

V

F

F

E

E

M

F

D

D

A

N

F

F

S

A

R

K

G

G

T

T

F

K

A
x
S

M

M

V

L

S

D

A

A

V

C

A

V

N

K

S

T

Y

C

A

E

-

A

-

G

-

N

L

V

T

V

I

I

V

V

G

G

D

D

T
|
T

D

A

V

T

A

V

V

A

H

K

R

K

A

Y

G

G

E

K

Y

E

A

D

K

A

I

L

S
|
S

Y

H

I

V

S
|
S

T

T

G

G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

A

L

L

P

P

G

G

I

V

K

V

V

A

L

L

Y75 (= Y73) modified: Phosphotyrosine
S76 (= S74) modified: Phosphoserine
S143 (vs. gap) modified: Phosphoserine
S172 (≠ A156) modified: Phosphoserine
S173 (= S157) modified: Phosphoserine
S183 (≠ A169) modified: Phosphoserine
S253 (= S239) modified: Phosphoserine
S260 (≠ L246) modified: Phosphoserine
T299 (= T285) modified: Phosphothreonine
S328 (≠ A313) modified: Phosphoserine
S351 (≠ A336) modified: Phosphoserine
T373 (= T355) modified: Phosphothreonine
S387 (= S369) modified: Phosphoserine
S390 (= S372) modified: Phosphoserine

Sites not aligning to the query:

412 modified: Phosphoserine
413 modified: Phosphoserine

2wzcA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and aluminium tetrafluoride (see paper)
47% identity, 96% coverage: 11:395/399 of query aligns to 11:404/405 of 2wzcA

query
sites
2wzcA

D

D

L

V

R

K

E

G

K

K

K

R

I

V

F

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

K

E

N

H

N

Q

Q

N

-

I

I

T

T

E

N

D

N

T

Q

R
|
R

I

I

R

K

A

A

V

A

L

V

P

P

T

S

I

I

N

K

Y

F

A

C

L

L

D

D

Y

N

N

G

A

A

K

K

-

S

V

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

-

V

E

P

R

M

N

P

A

D

K

K

Y

Y

S

S

M

L

A

E

P

P

A

V

A

A

K

V

R

E

L

L

S

K

R

S

L

L

G

G

K

K

E

D

V

V

K

L

L

F

A

L

P

K

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

A

S

N

M

P

Q

A

P

A

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

Y

H

-

V

-

E

-

G

P

K

G

G

E

E

E

P

K

A

N

K

D

I

D

E

D

A

F

F

A

R

K

A

A

S

L

L

A

S

D

K

H

L

C

G

D

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

V

T

N

K

-

F

-

F

L

P

P

I

Q

V

K

A

A

A

G

G

G

F

F

L

L

M

M

K

K

N

K

E

E

M

L

S

N

Y

Y

F

F

E

A

K

K

S

A

M

L

K

E

N

S

P

P

I

E

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

V

L

L

I

E

N

N

N

L

M

C

L

N

D

K

K

V

V

D

N

K

E

V

M

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

N

Y

M

N

E

V

I

G

G

K

T

S

S

L

L

V

F

E

D

E

E

N

E

L

G

L

A

E

K

T

I

A

V

L

K

N

D

T

L

Y

M

N

S

K

K

A

A

R

E

E

K

K

N

G

G

I

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

Q

K

F

F

N

D

P

E

A

N

A

A

E

K

T

T

K

G

V

Q

T

A

T

T

I

V

Q

A

E

S

-

G

I

I

P

P

E

A

G

G

W

W

M

M

A

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

T

K

L

K

F

Y

S

A

T

E

A

A

L

V

Q

T

N

R

A

A

K

K

T

Q

I

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

F

K

A

A

M

L

V

M

S

D

A

E

V

V

A

V

N

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

T

T

I

I

V

I

G

G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

V

C

H

A

R

K

A

W

G

N

E

T

Y

E

A

D

K

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

V

L

L

P

P

G

G

I

V

K

D

V

A

L

L

E

S

D

N

active site: R37 (= R38), K204 (= K200), G362 (= G353), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G198), A203 (= A199), K204 (= K200), K208 (= K204), G226 (= G222), G227 (= G223), N325 (= N319), P327 (= P321), G329 (= G323), V330 (= V324), E332 (= E326), G361 (= G352), D363 (= D354), T364 (= T355)
binding tetrafluoroaluminate ion: R37 (= R38), K204 (= K200), K208 (= K204), G361 (= G352), G362 (= G353), G384 (= G375)

2wzbA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp, 3pg and magnesium trifluoride (see paper)
47% identity, 96% coverage: 11:395/399 of query aligns to 11:404/405 of 2wzbA

query
sites
2wzbA

D

D

L

V

R

K

E

G

K

K

K

R

I

V

F

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

K

E

N

H

N

Q

Q

N

-

I

I

T

T

E

N

D

N

T

Q

R
|
R

I

I

R

K

A

A

V

A

L

V

P

P

T

S

I

I

N

K

Y

F

A

C

L

L

D

D

Y

N

N

G

A

A

K

K

-

S

V

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

-

V

E

P

R

M

N

P

A

D

K

K

Y

Y

S

S

M

L

A

E

P

P

A

V

A

A

K

V

R

E

L

L

S

K

R

S

L

L

G

G

K

K

E

D

V

V

K

L

L

F

A

L

P

K

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

A

S

N

M

P

Q

A

P

A

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

Y

H

-

V

-

E

-

G

P

K

G

G

E

E

E

P

K

A

N

K

D

I

D

E

D

A

F

F

A

R

K

A

A

S

L

L

A

S

D

K

H

L

C

G

D

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

V

T

N

K

-

F

-

F

L

P

P

I

Q

V

K

A

A

A

G

G

G

F

F

L

L

M

M

K

K

N

K

E

E

M

L

S

N

Y

Y

F

F

E

A

K

K

S

A

M

L

K

E

N

S

P

P

I

E

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

V

L

L

I

E

N

N

N

L

M

C

L

N

D

K

K

V

V

D

N

K

E

V

M

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

N

Y

M

N

E

V

I

G

G

K

T

S

S

L

L

V

F

E

D

E

E

N

E

L

G

L

A

E

K

T

I

A

V

L

K

N

D

T

L

Y

M

N

S

K

K

A

A

R

E

E

K

K

N

G

G

I

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

Q

K

F

F

N

D

P

E

A

N

A

A

E

K

T

T

K

G

V

Q

T

A

T

T

I

V

Q

A

E

S

-

G

I

I

P

P

E

A

G

G

W

W

M

M

A

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

T

K

L

K

F

Y

S

A

T

E

A

A

L

V

Q

T

N

R

A

A

K

K

T

Q

I

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

F

K

A

A

M

L

V

M

S

D

A

E

V

V

A

V

N

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

T

T

I

I

V

I

G

G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

V

C

H

A

R

K

A

W

G

N

E

T

Y

E

A

D

K

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

V

L

L

P

P

G

G

I

V

K

D

V

A

L

L

E

S

D

N

active site: R37 (= R38), K204 (= K200), G362 (= G353), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G198), A203 (= A199), K204 (= K200), K208 (= K204), G226 (= G222), G227 (= G223), N325 (= N319), P327 (= P321), G329 (= G323), V330 (= V324), E332 (= E326), G361 (= G352), D363 (= D354), T364 (= T355)
binding trifluoromagnesate: K204 (= K200), K208 (= K204), G361 (= G352), G384 (= G375), G385 (= G376)

4axxA The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp 3-phosphoglycerate and beryllium trifluoride
46% identity, 96% coverage: 11:395/399 of query aligns to 11:406/407 of 4axxA

query
sites
4axxA

D

D

L

V

R

K

E

G

K

K

K

R

I

V

F

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

K

E

N

H

N

Q

Q

N

-

I

I

T

T

E

N

D

N

T

Q

R
|
R

I

I

R

K

A

A

V

A

L

V

P

P

T

S

I

I

N

K

Y

F

A

C

L

L

D

D

Y

N

N

G

A

A

K

K

-

S

V

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

-

V

E

P

R

M

N

P

A

D

K

K

Y

Y

S

S

M

L

A

E

P

P

A

V

A

A

K

V

R

E

L

L

S

K

R

S

L

L

G

G

K

K

E

D

V

V

K

L

L

F

A

L

P

K

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

A

S

N

M

P

Q

A

P

A

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

Y

H

P

V

G

E

E

E

K

G

N

K

D

A

-

K

-

A

-

E

-

P

-

A

-

K

-

I

D

E

D

A

F

F

A

R

K

A

A

S

L

L

A

S

D

K

H

L

C

G

D

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

V

T

N

K

-

F

-

F

L

P

P

I

Q

V

K

A

A

A

G

G

G

F

F

L

L

M

M

K

K

N

K

E

E

M

L

S

N

Y

Y

F

F

E

A

K

K

S

A

M

L

K

E

N

S

P

P

I

E

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

V

L

L

I

E

N

N

N

L

M

C

L

N

D

K

K

V

V

D

N

K

E

V

M

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

N

Y

M

N

E

V

I

G

G

K

T

S

S

L

L

V

F

E

D

E

E

N

E

L

G

L

A

E

K

T

I

A

V

L

K

N

D

T

L

Y

M

N

S

K

K

A

A

R

E

E

K

K

N

G

G

I

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

Q

K

F

F

N

D

P

E

A

N

A

A

E

K

T

T

K

G

V

Q

T

A

T

T

I

V

Q

A

E

S

-

G

I

I

P

P

E

A

G

G

W

W

M

M

A

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

T

K

L

K

F

Y

S

A

T

E

A

A

L

V

Q

T

N

R

A

A

K

K

T

Q

I

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

F

K

A

A

M

L

V

M

S

D

A

E

V

V

A

V

N

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

T

T

I

I

V

I

G

G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

V

C

H

A

R

K

A

W

G

N

E

T

Y

E

A

D

K

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

V

L

L

P

P

G

G

I

V

K

D

V

A

L

L

E

S

D

N

active site: R37 (= R38), K206 (= K200), G364 (= G353), G387 (= G376)
binding adenosine-5'-diphosphate: G204 (= G198), A205 (= A199), K210 (= K204), G228 (= G222), G229 (= G223), N327 (= N319), P329 (= P321), G331 (= G323), V332 (= V324), E334 (= E326), G363 (= G352), G364 (= G353), D365 (= D354), T366 (= T355)
binding beryllium trifluoride ion: K206 (= K200), K210 (= K204), G363 (= G352)

2y3iA The structure of the fully closed conformation of human pgk in complex with l-adp, 3pg and the tsa aluminium tetrafluoride (see paper)
45% identity, 99% coverage: 1:395/399 of query aligns to 1:414/414 of 2y3iA

query
sites
2y3iA

M

M

S

S

I

N

R

K

Y

L

I

T

D

L

E

D

I

K

S

L

D

D

L

V

R

K

E

G

K

K

K

R

I

V

F

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

K

E

N

H

N

Q

Q

N

-

I

I

T

T

E

N

D

N

T

Q

R
|
R

I

I

R

K

A

A

V

A

L

V

P

P

T

S

I

I

N

K

Y

F

A

C

L

L

D

D

Y

N

N

G

A

A

K

K

-

S

V

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

-

V

E

P

R

M

N

P

A

D

K

K

Y

Y

S

S

M

L

A

E

P

P

A

V

A

A

K

V

R

E

L

L

S

K

R

S

L

L

G

G

K

K

E

D

V

V

K

L

L

F

A

L

P

K

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

A

S

N

M

P

Q

A

P

A

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

Y

H

P

V

G

E

E

E

K

G

N

K

D

G

D

K

D

D

-

A

-

S

-

G

-

N

-

K

-

V

-

K

-

A

-

E

-

P

-

A

-

K

-

I

-

E

-

A

F

F

A

R

K

A

A

S

L

L

A

S

D

K

H

L

C

G

D

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

V

T

N

K

-

F

-

F

L

P

P

I

Q

V

K

A

A

A

G

G

G

F

F

L

L

M

M

K

K

N

K

E

E

M

L

S

N

Y

Y

F

F

E

A

K

K

S

A

M

L

K

E

N

S

P

P

I

E

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K

K

L

I

E

Q

V

L

L

I

E

N

N

N

L

M

C

L

N

D

K

K

V

V

D

N

K

E

V

M

I

I

G

G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

N

Y

M

N

E

V

I

G

G

K

T

S

S

L

L

V

F

E

D

E

E

N

E

L

G

L

A

E

K

T

I

A

V

L

K

N

D

T

L

Y

M

N

S

K

K

A

A

R

E

E

K

K

N

G

G

I

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

Q

K

F
|
F

N

D

P

E

A

N

A

A

E

K

T

T

K

G

V

Q

T

A

T

T

I

V

Q

A

E

S

-

G

I

I

P

P

E

A

G

G

W

W

M

M

A

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

T

K

L

K

F

Y

S

A

T

E

A

A

L

V

Q

T

N

R

A

A

K

K

T

Q

I

I

V

V

W

W

N
|
N

G

G

P

P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

F

K

A

A

M

L

V

M

S

D

A

E

V

V

A

V

N

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

T

T

I

I

V

I

G

G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

V

C

H

A

R

K

A

W

G

N

E

T

Y

E

A

D

K

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

V

L

L

P

P

G

G

I

V

K

D

V

A

L

L

E

S

D

N

active site: R37 (= R38), K214 (= K200), G372 (= G353), G395 (= G376)
binding tetrafluoroaluminate ion: K214 (= K200), G371 (= G352), G372 (= G353), G394 (= G375)
binding l-adenosine-5'-diphosphate: G212 (= G198), A213 (= A199), F290 (= F275), N335 (= N319), G339 (= G323), V340 (= V324), E342 (= E326), G371 (= G352), G372 (= G353), D373 (= D354), T374 (= T355)

2x15A The catalytically active fully closed conformation of human phosphoglycerate kinase in complex with adp and 1,3- bisphosphoglycerate
46% identity, 96% coverage: 11:395/399 of query aligns to 11:407/408 of 2x15A

query
sites
2x15A

D

D

L

V

R

K

E

G

K

K

K

R

I

V

F

V

M

M

R

R

V

V

D
|
D

F

F

N
|
N

V

V

P

P

L

M

D

K

E

N

H

N

Q

Q

N

-

I

I

T

T

E

N

D

N

T

Q

R
|
R

I

I

R

K

A

A

V

A

L

V

P

P

T

S

I

I

N

K

Y

F

A

C

L

L

D

D

Y

N

N

G

A

A

K

K

-

S

V

V

L

L

A

M

S

S

H
|
H

L

L

G

G

R
|
R

P

P

K

D

G

G

-

V

E

P

R

M

N

P

A

D

K

K

Y

Y

S

S

M

L

A

E

P

P

A

V

A

A

K

V

R

E

L

L

S

K

R

S

L

L

G

G

K

K

E

D

V

V

K

L

L

F

A

L

P

K

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

A

S

N

M

P

Q

A

P

A

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R
|
R

F

F

Y

H

P

V

G

E

E

E

K

G

N

K

D

G

-

K

-

A

-

E

-

P

-

A

-

K

-

I

D

E

D

A

F

F

A

R

K

A

A

S

L

L

A

S

D

K

H

L

C

G

D

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R
|
R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

V

T

N

K

-

F

-

F

L

P

P

I

Q

V

K

A

A

A

G

G

G

F

F

L

L

M

M

K

K

N

K

E

E

M

L

S

N

Y

Y

F

F

E

A

K

K

S

A

M

L

K

E

N

S

P

P

I

E

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

V

L

L

I

E

N

N

N

L

M

C

L

N

D

K

K

V

V

D

N

K

E

V

M

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

N

Y

M

N

E

V

I

G

G

K

T

S

S

L

L

V

F

E

D

E

E

N

E

L

G

L

A

E

K

T

I

A

V

L

K

N

D

T

L

Y

M

N

S

K

K

A

A

R

E

E

K

K

N

G

G

I

V

K

K

F

I

Y

T

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

Q

K

F

F

N

D

P

E

A

N

A

A

E

K

T

T

K

G

V

Q

T

A

T

T

I

V

Q

A

E

S

-

G

I

I

P

P

E

A

G

G

W

W

M

M

A

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

T

K

L

K

F

Y

S

A

T

E

A

A

L

V

Q

T

N

R

A

A

K

K

T

Q

I

I

V

V

W

W

N
|
N

G

G

P
|
P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

F

K

A

A

M

L

V

M

S

D

A

E

V

V

A

V

N

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

T

T

I

I

V

I

G

G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

V

C

H

A

R

K

A

W

G

N

E

T

Y

E

A

D

K

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

V

L

L

P

P

G

G

I

V

K

D

V

A

L

L

E

S

D

N

active site: R37 (= R38), K207 (= K200), G365 (= G353), G388 (= G376)
binding adenosine-5'-diphosphate: G205 (= G198), A206 (= A199), K207 (= K200), K211 (= K204), G229 (= G222), G230 (= G223), N328 (= N319), P330 (= P321), G332 (= G323), V333 (= V324), E335 (= E326), G364 (= G352), G365 (= G353), D366 (= D354), T367 (= T355)
binding adenosine-5'-triphosphate: G205 (= G198), A206 (= A199), K207 (= K200), K211 (= K204), G229 (= G222), G230 (= G223), N328 (= N319), G332 (= G323), V333 (= V324), E335 (= E326), G364 (= G352), G365 (= G353), D366 (= D354), T367 (= T355), G387 (= G375), G388 (= G376)
binding 1,3-bisphosphoglyceric acid: D22 (= D22), N24 (= N24), R37 (= R38), H61 (= H61), R64 (= R64), R121 (= R120), R162 (= R153), K207 (= K200), K211 (= K204), G364 (= G352), G387 (= G375), G388 (= G376)

P00558 Phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2; EC 2.7.2.3 from Homo sapiens (Human) (see 16 papers)
46% identity, 96% coverage: 11:395/399 of query aligns to 13:416/417 of P00558

query
sites
P00558

D

D

L

V

R

K

E

G

K

K

K

R

I

V

F

V

M

M

R

R

V

V

D
|
D

F
|
F

N
|
N

V

V

P

P

L

M

D

K

E

N

H

N

Q

Q

N

-

I

I

T

T

E

N

D

N

T

Q

R
|
R

I

I

R

K

A

A

V

A

L

V

P

P

T

S

I

I

N

K

Y

F

A

C

L

L

D

D

Y

N

N

G

A

A

K

K

-

S

V

V

L

L

A

M

S

S

H
|
H

L
|
L

G
|
G

R
|
R

P

P

K

D

G

G

-

V

E

P

R

M

N

P

A

D

K

K

Y

Y

S

S

M

L

A

E

P

P

A

V

A

A

K

V

R

E

L

L

S

K

R

S

L
|
L

L

L

G

G

K

K

E

D

V

V

K

L

L

F

A

L

P
x
K

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

A

S

N

M

P

Q

A

P

A

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R
|
R

F

F

Y

H

P

V

G

E

E

E

K

G

N
x
K

D

G

D

K

D

D

-

A

-

S

-

G

-

N

-

K

-

V

-

K

-

A

-

E

-

P

-

A

-

K

-

I

-

E

-

A

F

F

A

R

K

A

A

S

L

L

A

S

D

K

H

L

C
x
G

D

D

I

V

Y

Y

V

V

N

N

D
|
D

A

A

F

F

A

G

V

T

S

A

H

H

R
|
R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

V

T

N

K

-

F

-

F

L

P

P

I

Q

V

K

A

A

A

G

G

G

F

F

L

L

M

M

K
|
K

N

K

E

E

M

L

S

N

Y

Y

F

F

E

A

K

K

S

A

M

L

K

E

N

S

P

P

I

E

R
|
R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

A

A

K
|
K

V

V

S

A

G

D

K
|
K

L

I

E

Q

V

L

L

I

E

N

N

N

L

M

C

L

N

D

K

K

V

V

D

N

K

E

V

M

I

I

G

G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

N

Y

M

N
x
E

V

I

G

G

K

T

S

S

L

L

V

F

E

D

E

E

N

E

L

G

L

A

E

K

T

I

A
x
V

L

K

N
x
D

T

L

Y

M

N

S

K

K

A

A

R

E

E

K

K

N

G

G

I

V

K

K

F

I

Y

T

L

L

P

P

V

V

D
|
D

C

F

V

V

V

T

A

A

D

D

Q

K

F

F

N

D

P

E

A

N

A

A

E

K

T

T

K

G

V

Q

T

A

T

T

I

V

Q

A

E

S

-

G

I

I

P

P

E

A

G

G

W

W

M

M

A
x
G

L

L

D
|
D

I
x
C

G

G

P

P

A

E

T

S

V

S

T

K

L
x
K

F

Y

S

A

T

E

A

A

L

V

Q

T

N

R

A

A

K

K

T

Q

I

I

V

V

W

W

N

N

G

G

P

P

M

V

G

G

V

V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T
|
T

F

K

A

A

M

L

V

M

S

D

A

E

V

V

A

V

N

K

S

A

Y

T

A

S

-

R

-

G

-

C

L

I

T

T

I

I

V

I

G

G

G
|
G

D
|
D

T
|
T

D

A

V

T

A

C

V

C

H

A

R

K

A

W

G

N

E

T

Y

E

A

D

K

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

V

L

L

P

P

G

G

I

V

K

D

V

A

L

L

E

S

D

N

DFN 24:26 (= DFN 22:24) binding substrate
R39 (= R38) binding substrate
HLGR 63:66 (= HLGR 61:64) binding substrate
L88 (= L85) to P: in PGK1D; with congenital non-spherocytic anemia; variant Matsue; dbSNP:rs137852531
K97 (≠ P94) modified: N6-(2-hydroxyisobutyryl)lysine; alternate
R123 (= R120) binding substrate
K131 (≠ N128) modified: N6-malonyllysine; alternate
G158 (≠ C140) to V: in PGK1D; with chronic hemolytic anemia; variant Shizuoka; dbSNP:rs137852532
D164 (= D146) to V: in PGK1D; with chronic hemolytic anemia and intellectual disability; variant Amiens; dbSNP:rs137852538
R171 (= R153) binding substrate
K191 (= K175) natural variant: Missing (in PGK1D; with chronic hemolytic anemia; variant Alabama)
R206 (= R190) to P: in PGK1D; with chronic hemolytic anemia; variant Uppsala; dbSNP:rs137852529
K216 (= K200) modified: N6-(2-hydroxyisobutyryl)lysine
K220 (= K204) binding ATP; modified: N6-(2-hydroxyisobutyryl)lysine
E252 (≠ N235) to A: in PGK1D; with chronic hemolytic anemia; variant Antwerp
V266 (≠ A249) to M: in PGK1D; with chronic non-spherocytic hemolytic anemia; variant Tokyo; dbSNP:rs431905501
D268 (≠ N251) to N: in Munchen; 21% of activity; dbSNP:rs137852528
D285 (= D268) to V: in PGK1D; with chronic hemolytic anemia; variant Herlev; 50% of activity; dbSNP:rs137852535
G313 (≠ A295) binding ATP
D315 (= D297) to N: in PGK1D; with rhabdomyolysis; variant Creteil; dbSNP:rs2149136994
C316 (≠ I298) to R: in PGK1D; with chronic hemolytic anemia; variant Michigan; dbSNP:rs137852533
K323 (≠ L305) modified: N6-(2-hydroxyisobutyryl)lysine
E344 (= E326) binding ATP
T352 (= T334) to N: in dbSNP:rs137852530
GGDT 373:376 (= GGDT 352:355) binding ATP

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

2wzdA The catalytically active fully closed conformation of human phosphoglycerate kinase k219a mutant in complex with adp, 3pg and aluminium trifluoride (see paper)
46% identity, 96% coverage: 11:395/399 of query aligns to 11:404/405 of 2wzdA

query
sites
2wzdA

D

D

L

V

R

K

E

G

K

K

K

R

I

V

F

V

M

M

R

R

V

V

D

D

F

F

N

N

V

V

P

P

L

M

D

K

E

N

H

N

Q

Q

N

-

I

I

T

T

E

N

D

N

T

Q

R
|
R

I

I

R

K

A

A

V

A

L

V

P

P

T

S

I

I

N

K

Y

F

A

C

L

L

D

D

Y

N

N

G

A

A

K

K

-

S

V

V

L

L

A

M

S

S

H

H

L

L

G

G

R

R

P

P

K

D

G

G

-

V

E

P

R

M

N

P

A

D

K

K

Y

Y

S

S

M

L

A

E

P

P

A

V

A

A

K

V

R

E

L

L

S

K

R

S

L

L

G

G

K

K

E

D

V

V

K

L

L

F

A

L

P

K

D

D

C

C

I

V

G

G

D

P

G

E

V

V

S

E

K

K

I

A

I

C

D

A

S

N

M

P

Q

A

P

A

G

G

D

S

V

V

V

I

M

L

L

L

E

E

N

N

L

L

R

R

F

F

Y

H

-

V

-

E

-

G

P

K

G

G

E

E

E

P

K

A

N

K

D

I

D

E

D

A

F

F

A

R

K

A

A

S

L

L

A

S

D

K

H

L

C

G

D

D

I

V

Y

Y

V

V

N

N

D

D

A

A

F

F

A

G

V

T

S

A

H

H

R

R

A

A

H

H

A

S

S

S

V

M

E

V

A

G

I

V

T

N

K

-

F

-

F

L

P

P

I

Q

V

K

A

A

A

G

G

G

F

F

L

L

M

M

K

K

N

K

E

E

M

L

S

N

Y

Y

F

F

E

A

K

K

S

A

M

L

K

E

N

S

P

P

I

E

R

R

P

P

L

F

V

L

A

A

I

I

L

L

G

G

G
|
G

A
|
A

K
|
K

V

V

S

A

G

D

K

A

L

I

E

Q

V

L

L

I

E

N

N

N

L

M

C

L

N

D

K

K

V

V

D

N

K

E

V

M

I

I

G

G

G
|
G

M

M

A

A

F

F

T

T

F

F

L

L

K

K

A

V

L

L

-

N

G

N

Y

M

N

E

V

I

G

G

K

T

S

S

L

L

V

F

E

D

E

E

N

E

L

G

L

A

E

K

T

I

A

V

L

K

N

D

T

L

Y

M

N

S

K

K

A

A

R

E

E

K

K

N

G

G

I

V

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K

F

I

Y

T

L

L

P

P

V

V

D

D

C

F

V

V

V

T

A

A

D

D

Q

K

F

F

N

D

P

E

A

N

A

A

E

K

T

T

K

G

V

Q

T

A

T

T

I

V

Q

A

E

S

-

G

I

I

P

P

E

A

G

G

W

W

M

M

A

G

L

L

D

D

I

C

G

G

P

P

A

E

T

S

V

S

T

K

L

K

F

Y

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A

T

E

A

A

L

V

Q

T

N

R

A

A

K

K

T

Q

I

I

V

V

W

W

N
|
N

G

G

P
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P

M

V

G
|
G

V
|
V

F

F

E
|
E

M

W

D

E

A

A

F

F

S

A

R

R

G

G

T

T

F

K

A

A

M

L

V

M

S

D

A

E

V

V

A

V

N

K

S

A

Y

T

A

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-

R

-

G

-

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L

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T

T

I

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G

G

G
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G

D
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D

T
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T

D

A

V

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A

C

V

C

H

A

R

K

A

W

G

N

E

T

Y

E

A

D

K

K

I

V

S

S

Y

H

I

V

S

S

T

T

G

G

G
|
G

A

A

F

S

L

L

E

E

L

L

E

E

G

G

K

K

K

V

L

L

P

P

G

G

I

V

K

D

V

A

L

L

E

S

D

N

active site: R37 (= R38), K204 (= K200), G362 (= G353), G385 (= G376)
binding adenosine-5'-diphosphate: G202 (= G198), A203 (= A199), K204 (= K200), G226 (= G222), G227 (= G223), N325 (= N319), P327 (= P321), G329 (= G323), V330 (= V324), E332 (= E326), G361 (= G352), D363 (= D354), T364 (= T355)
binding aluminum fluoride: R37 (= R38), K204 (= K200), G361 (= G352), G362 (= G353), G384 (= G375)

Query Sequence

>WP_011938952.1 NCBI__GCF_000016745.1:WP_011938952.1
MSIRYIDEISDLREKKIFMRVDFNVPLDEHQNITEDTRIRAVLPTINYALDYNAKVVLAS
HLGRPKGERNAKYSMAPAAKRLSRLLGKEVKLAPDCIGDGVSKIIDSMQPGDVVMLENLR
FYPGEEKNDDDFAKALADHCDIYVNDAFAVSHRAHASVEAITKFFPIVAAGFLMKNEMSY
FEKSMKNPIRPLVAILGGAKVSGKLEVLENLCNKVDKVIIGGGMAFTFLKALGYNVGKSL
VEENLLETALNTYNKAREKGIKFYLPVDCVVADQFNPAAETKVTTIQEIPEGWMALDIGP
ATVTLFSTALQNAKTIVWNGPMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDVAVH
RAGEYAKISYISTGGGAFLELLEGKKLPGIKVLEDNGHK

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory