SitesBLAST
Comparing WP_011939533.1 NCBI__GCF_000016745.1:WP_011939533.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
40% identity, 98% coverage: 3:286/289 of query aligns to 5:285/287 of 1nvtB
- active site: K75 (= K73), D111 (= D109)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (= I70), G135 (= G134), G137 (= G136), G138 (= G137), A139 (= A138), N157 (= N158), R158 (= R159), T159 (= T160), K162 (= K163), A200 (≠ T203), T201 (= T204), P202 (≠ S205), I203 (≠ V206), M205 (= M208), L229 (≠ M230), Y231 (= Y232), M255 (= M256), L256 (= L257)
- binding zinc ion: E22 (≠ N20), H23 (= H21)
1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
40% identity, 98% coverage: 3:286/289 of query aligns to 5:285/287 of 1nvtA
- active site: K75 (= K73), D111 (= D109)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (= G134), A139 (= A138), N157 (= N158), R158 (= R159), T159 (= T160), K162 (= K163), A200 (≠ T203), T201 (= T204), P202 (≠ S205), I203 (≠ V206), M205 (= M208), L229 (≠ M230), Y231 (= Y232), G252 (= G253), M255 (= M256), L256 (= L257)
Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
40% identity, 98% coverage: 5:286/289 of query aligns to 2:280/282 of Q58484
2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
41% identity, 93% coverage: 5:274/289 of query aligns to 2:256/267 of 2hk9B
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ I70), G130 (= G134), G133 (= G137), A134 (= A138), N153 (= N158), R154 (= R159), T155 (= T160), K158 (= K163), T188 (= T204), S189 (= S205), V190 (= V206), I214 (≠ M230), M238 (= M256), L239 (= L257)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S22), S21 (= S24), N64 (= N67), T66 (= T69), K70 (= K73), N91 (= N94), D106 (= D109), Y216 (= Y232), L239 (= L257), Q242 (= Q260)
2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
41% identity, 93% coverage: 5:274/289 of query aligns to 2:256/269 of 2hk9A
- binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ I70), G132 (= G136), G133 (= G137), A134 (= A138), N153 (= N158), R154 (= R159), T155 (= T160), T188 (= T204), S189 (= S205), V190 (= V206)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S22), S21 (= S24), N64 (= N67), K70 (= K73), N91 (= N94), D106 (= D109), Y216 (= Y232), L239 (= L257), Q242 (= Q260)
O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
41% identity, 93% coverage: 5:274/289 of query aligns to 2:256/269 of O67049
- SLS 19:21 (= SLS 22:24) binding shikimate
- D82 (≠ P85) binding NADP(+)
- N91 (= N94) binding shikimate
- D106 (= D109) binding shikimate
- GAGGA 130:134 (= GAGGA 134:138) binding NADP(+)
- I214 (≠ M230) binding NADP(+)
- Y216 (= Y232) binding shikimate
- G235 (= G253) binding NADP(+)
- Q242 (= Q260) binding shikimate
3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
37% identity, 97% coverage: 3:283/289 of query aligns to 7:291/291 of 3tozA
- binding nicotinamide-adenine-dinucleotide: G137 (= G134), A138 (= A135), G139 (= G136), G140 (= G137), A141 (= A138), N161 (= N158), R162 (= R159), D164 (≠ R161), F166 (≠ K163), T210 (= T204), G211 (≠ S205), V212 (= V206), M214 (= M208), F217 (= F213), V238 (≠ M230), Y240 (= Y232), G261 (= G253), M264 (= M256), M265 (≠ L257)
Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
37% identity, 97% coverage: 3:283/289 of query aligns to 7:291/291 of Q8Y9N5
3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
37% identity, 97% coverage: 3:283/289 of query aligns to 4:288/288 of 3tnlA
- binding nicotinamide-adenine-dinucleotide: M71 (≠ I70), G134 (= G134), A135 (= A135), G136 (= G136), G137 (= G137), A138 (= A138), N158 (= N158), R159 (= R159), D161 (≠ R161), F163 (≠ K163), T207 (= T204), V209 (= V206), M211 (= M208), F214 (= F213), V235 (≠ M230), Y237 (= Y232), M261 (= M256), M262 (≠ L257)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (= S22), S25 (= S24), N68 (= N67), S70 (≠ T69), K74 (= K73), N95 (= N94), D110 (= D109), Q265 (= Q260)
Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
34% identity, 96% coverage: 12:289/289 of query aligns to 3:268/269 of Q5HNV1
- SLS 13:15 (= SLS 22:24) binding shikimate
- T60 (= T69) binding shikimate
- N85 (= N94) binding shikimate
- D100 (= D109) binding shikimate
- Y211 (= Y232) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
- Q239 (= Q260) binding shikimate
1npdB X-ray structure of shikimate dehydrogenase complexed with NAD+ from e.Coli (ydib) northeast structural genomics research consortium (nesg) target er24 (see paper)
34% identity, 96% coverage: 5:282/289 of query aligns to 3:284/288 of 1npdB
- binding nicotinamide-adenine-dinucleotide: A132 (= A135), G133 (= G136), G134 (= G137), A135 (= A138), N155 (= N158), R156 (= R159), D158 (≠ R161), F160 (≠ K163), T204 (= T204), K205 (≠ S205), V206 (= V206), M208 (= M208), C232 (≠ M230), M258 (= M256), L259 (= L257)
P0A6D5 Quinate/shikimate dehydrogenase; NAD-dependent shikimate 5-dehydrogenase; EC 1.1.1.282 from Escherichia coli (strain K12) (see 4 papers)
34% identity, 96% coverage: 5:282/289 of query aligns to 3:284/288 of P0A6D5
- S22 (= S24) mutation to A: Kinetically unchanged as compared with the wild-type.
- Y39 (= Y41) mutation to F: Kinetically unchanged as compared with the wild-type.
- S67 (≠ T69) mutation to A: Reduces activity towards quinate about 6-fold, but has a little effect on shikimate conversion.
- K71 (= K73) mutation to A: 3200-fold decrease in the affinity for quinate. 170-fold decrease in the affinity for shikimate.; mutation to G: 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
- N92 (= N94) mutation to A: Alters protein structure. Loss of activity for both substrates.
- T106 (= T108) mutation to A: 2000-fold decrease in the affinity for quinate. 70-fold decrease in the affinity for shikimate. 10-fold greater reduction in catalytic efficiency is observed with quinate than with shikimate.
- D107 (= D109) mutation to A: Loss of activity towards quinate. 20000-fold decrease in the affinity for shikimate.
- AGGA 132:135 (= AGGA 135:138) binding NAD(+)
- NRRD 155:158 (≠ NRTR 158:161) binding NAD(+)
- K205 (≠ S205) binding NAD(+)
- CVYN 232:235 (≠ MVYA 230:233) binding NAD(+)
- G255 (= G253) binding NAD(+)
- Q262 (= Q260) mutation to A: 3-fold reduction in catalytic efficiency for both substrates.
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1o9bA Quinate/shikimate dehydrogenase ydib complexed with nadh (see paper)
35% identity, 94% coverage: 10:282/289 of query aligns to 2:278/280 of 1o9bA
- binding 1,4-dihydronicotinamide adenine dinucleotide: A126 (= A135), G127 (= G136), G128 (= G137), A129 (= A138), R150 (= R159), F154 (≠ K163), K199 (≠ S205), V200 (= V206), M202 (= M208), C226 (≠ M230), Y228 (= Y232), M252 (= M256), L253 (= L257)
3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
34% identity, 96% coverage: 12:287/289 of query aligns to 3:257/258 of 3dooA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (= S22), S15 (= S24), N58 (= N67), T60 (= T69), K64 (= K73), N85 (= N94), D100 (= D109), F227 (≠ L257), Q230 (= Q260)
Q8ZPR4 Quinate/shikimate dehydrogenase; NAD-dependent shikimate 5-dehydrogenase; EC 1.1.1.282 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
35% identity, 96% coverage: 5:282/289 of query aligns to 3:284/288 of Q8ZPR4
P15770 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Escherichia coli (strain K12) (see paper)
36% identity, 91% coverage: 10:273/289 of query aligns to 2:257/272 of P15770
1nytA Shikimate dehydrogenase aroe complexed with NADP+ (see paper)
36% identity, 91% coverage: 10:273/289 of query aligns to 2:257/271 of 1nytA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K65 (= K73), D102 (= D109), G128 (= G136), G129 (= G137), A130 (= A138), N149 (= N158), R150 (= R159), T151 (= T160), R154 (≠ K163), T188 (= T204), S189 (= S205), S190 (≠ V206), M213 (= M230), G237 (= G253), M240 (= M256), L241 (= L257)
2ev9B Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP(h) and shikimate (see paper)
39% identity, 93% coverage: 12:281/289 of query aligns to 4:257/263 of 2ev9B
- active site: K64 (= K73), D100 (= D109)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S22), S16 (= S24), N58 (= N67), T60 (= T69), K64 (= K73), N85 (= N94), D100 (= D109), Q235 (= Q260)
Q5SJF8 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
39% identity, 93% coverage: 12:281/289 of query aligns to 4:257/263 of Q5SJF8
- SLS 14:16 (= SLS 22:24) binding shikimate
- T60 (= T69) binding shikimate
- K64 (= K73) active site, Proton acceptor
- N85 (= N94) binding shikimate
- D100 (= D109) binding shikimate
- GAGGA 123:127 (= GAGGA 134:138) binding NADP(+)
- NRT---------------PQR 146:151 (≠ NRTRNKGEELLSCFKSVFPQV 158:178) binding NADP(+)
- L205 (≠ M230) binding NADP(+)
- Y207 (= Y232) binding shikimate
- G228 (= G253) binding NADP(+)
- Q235 (= Q260) binding shikimate
2cy0A Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP (see paper)
39% identity, 93% coverage: 12:281/289 of query aligns to 4:257/262 of 2cy0A
- active site: K64 (= K73), D100 (= D109)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G123 (= G134), G126 (= G137), A127 (= A138), N146 (= N158), R147 (= R159), T148 (= T160), R151 (≠ V178), T179 (= T204), R180 (≠ S205), V181 (= V206), L205 (≠ M230), L232 (= L257)
Query Sequence
>WP_011939533.1 NCBI__GCF_000016745.1:WP_011939533.1
MKSRITGQTKVLGIIGSPINHSLSPLMQNAAIEAVGLDFVYVPFAVPADGLCAAVQGLRN
LGVAGFNVTIPFKTAIIPYLDKLSPEAELIGAVNTVNREGGFLVGYNTDGAGLLKSLAED
LDFTPKGAHVLILGAGGAARGAIASLCKAGVESVIIANRTRNKGEELLSCFKSVFPQVKL
ALSSLDVLGSSDLLHGIDLIVNTTSVGMDNTSFDGLDMSLMNPATRIYDMVYAPLETPFL
SAAKAMGLKSANGIGMLAAQGEIAFAVWTGCAPPSGLMKSCLLTAVKAK
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory