SitesBLAST
Comparing WP_011951898.1 NCBI__GCF_000016765.1:WP_011951898.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
Q4W6G0 Quinohemoprotein alcohol dehydrogenase ADH-IIG; ADH IIG; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 2 papers)
26% identity, 96% coverage: 11:655/669 of query aligns to 13:574/718 of Q4W6G0
- C138 (≠ P144) modified: Disulfide link with 139
- C139 (≠ D145) modified: Disulfide link with 138
- R144 (= R150) binding pyrroloquinoline quinone
- T189 (≠ L215) binding pyrroloquinoline quinone
- GA 205:206 (≠ RA 237:238) binding pyrroloquinoline quinone
- E207 (≠ N239) binding Ca(2+)
- T264 (≠ N284) binding pyrroloquinoline quinone
- N284 (≠ S304) binding Ca(2+)
- D329 (= D347) binding Ca(2+)
- K356 (= K374) binding pyrroloquinoline quinone
- W415 (≠ S469) binding substrate
- DW 419:420 (≠ NW 473:474) binding pyrroloquinoline quinone
Sites not aligning to the query:
- 1:29 signal peptide
- 30:718 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH-IIG
- 635 binding covalent
- 638 binding covalent
- 639 binding axial binding residue
- 676 binding axial binding residue
1kv9A Structure at 1.9 a resolution of a quinohemoprotein alcohol dehydrogenase from pseudomonas putida hk5 (see paper)
26% identity, 91% coverage: 39:648/669 of query aligns to 8:521/664 of 1kv9A
- active site: E173 (≠ D234), N250 (≠ S304), D295 (= D347)
- binding acetone: E173 (≠ D234), D295 (= D347)
- binding calcium ion: E173 (≠ D234), N250 (≠ S304), D295 (= D347)
- binding heme c: A101 (≠ R139), R102 (≠ S140)
- binding pyrroloquinoline quinone: E59 (= E100), C105 (≠ L143), C106 (≠ P144), R111 (= R150), T155 (≠ L215), G170 (≠ S231), A172 (≠ L233), E173 (≠ D234), T230 (≠ N284), W232 (= W286), K322 (= K374), N382 (= N473), W383 (= W474), W460 (≠ F578)
Sites not aligning to the query:
- binding heme c: 590, 591, 594, 595, 605, 606, 608, 611, 615, 619, 623, 631, 633, 636
- binding pyrroloquinoline quinone: 525
Q8GR64 Quinohemoprotein alcohol dehydrogenase ADH IIB; ADH IIB; Alcohol dehydrogenase (azurin); EC 1.1.9.1 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
26% identity, 91% coverage: 39:648/669 of query aligns to 30:543/690 of Q8GR64
- E81 (= E100) binding pyrroloquinoline quinone
- C127 (≠ L143) modified: Disulfide link with 128
- C128 (≠ P144) modified: Disulfide link with 127
- R133 (= R150) binding pyrroloquinoline quinone
- T177 (≠ L215) binding pyrroloquinoline quinone
- GA 193:194 (≠ VL 232:233) binding pyrroloquinoline quinone
- E195 (≠ D234) binding Ca(2+)
- T252 (≠ N284) binding pyrroloquinoline quinone
- N272 (≠ S304) binding Ca(2+)
- D317 (= D347) binding Ca(2+)
- K344 (= K374) binding pyrroloquinoline quinone
- NW 404:405 (= NW 473:474) binding pyrroloquinoline quinone
Sites not aligning to the query:
- 1:22 signal peptide
- 23:690 modified: mature protein, Quinohemoprotein alcohol dehydrogenase ADH IIB
- 547 binding pyrroloquinoline quinone
- 613 binding covalent
- 616 binding covalent
- 617 binding axial binding residue
- 655 binding axial binding residue
1yiqA Molecular cloning and structural analysis of quinohemoprotein alcohol dehydrogenase adhiig from pseudomonas putida hk5. Compariison to the other quinohemoprotein alcohol dehydrogenase adhiib found in the same microorganism. (see paper)
26% identity, 91% coverage: 49:655/669 of query aligns to 22:545/684 of 1yiqA
- active site: E178 (≠ N239), N255 (≠ S304), D300 (= D347)
- binding calcium ion: E178 (≠ N239), N255 (≠ S304), D300 (= D347)
- binding pyrroloquinoline quinone: E63 (= E100), C109 (≠ P144), C110 (≠ D145), R115 (= R150), T160 (≠ L215), G175 (≠ V236), G176 (≠ R237), A177 (= A238), E178 (≠ N239), T235 (≠ N284), W237 (= W286), K327 (= K374), D390 (≠ N473), W391 (= W474), F477 (≠ N569), A542 (≠ W652)
Sites not aligning to the query:
- binding heme c: 605, 606, 608, 609, 610, 623, 626, 630, 634, 637, 638, 642, 645, 646, 647, 648, 650
6damA Crystal structure of lanthanide-dependent methanol dehydrogenase xoxf from methylomicrobium buryatense 5g (see paper)
26% identity, 94% coverage: 41:667/669 of query aligns to 8:562/563 of 6damA