SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011952050.1 NCBI__GCF_000016765.1:WP_011952050.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O59010 Glutamate transporter homolog; Glt(Ph); Sodium-aspartate symporter Glt(Ph); Sodium-dependent aspartate transporter from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see 3 papers)
33% identity, 95% coverage: 3:404/421 of query aligns to 6:417/425 of O59010

query
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O59010

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S65 (≠ T62) mutation to V: Strongly decreased chloride conductance.
R276 (≠ A269) mutation to S: Increased rate of aspartate transport; when associated with R-395.
RSS 276:278 (≠ ASS 269:271) binding L-aspartate
M311 (≠ L304) mutation to A: Decreased dependence of aspartate binding on Na(+) concentration.
T314 (≠ S307) binding L-aspartate
V355 (= V348) binding L-aspartate
D394 (= D381) binding L-aspartate
M395 (= M382) mutation to R: Increased rate of aspartate transport; when associated with S-276.
R397 (= R384) mutation to A: Strongly decreased affinity for aspartate.
N401 (= N388) binding L-aspartate
D405 (≠ N392) mutation to N: Strongly decreased affinity for aspartate.

6x15A Inward-facing state of the glutamate transporter homologue gltph in complex with l-aspartate and sodium ions (see paper)
33% identity, 95% coverage: 3:404/421 of query aligns to 6:417/419 of 6x15A

query
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6x15A

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binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y7 (≠ R4), F46 (≠ L43), F46 (≠ L43), P75 (≠ T73), L91 (≠ T89), F95 (≠ M93), L130 (= L131), I133 (≠ F134), I159 (≠ V160), Y167 (vs. gap), K196 (≠ Q189), G200 (≠ Y193), I207 (≠ F200), F210 (= F203), L250 (≠ C243), I262 (= I255), M269 (≠ I262), T334 (= T327), V335 (≠ F328), G336 (≠ A329), T340 (= T333), L343 (= L336), M399 (≠ A386)
binding aspartic acid: S277 (= S270), S278 (= S271), T314 (≠ S307), G354 (= G347), A358 (= A351), G359 (≠ S352), D394 (= D381), R397 (= R384), T398 (≠ S385)
binding sodium ion: Y89 (≠ F87), T92 (≠ A90), S93 (= S91), G306 (= G299), T308 (≠ S301), N310 (= N303), N310 (= N303), M311 (≠ L304), D312 (= D305), S349 (= S342), I350 (≠ K343), T352 (≠ I345), N401 (= N388), V402 (= V389), D405 (≠ N392)

Sites not aligning to the query:

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: 4

6x14A Inward-facing state of the glutamate transporter homologue gltph in complex with tfb-tboa (see paper)
33% identity, 95% coverage: 3:403/421 of query aligns to 3:413/413 of 6x14A

query
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6x14A

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A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

T

T

F

V

A

G

H

Q

Q

Q

V

L

T

T

M

I

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K
x
I

G

G

I

T

A

A

G

G

V

V

P

P

R

G

A

A

S
x
G

L

A

V

I

I

M

I

L

S

C

S

M

T

V

L

L

A

H

F

S

F

V

D

G

I

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

E

A

A

A

G

A

L

Y

L

A

L

M

I

I

L
|
L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
|
R

S

T

A

M

T

V

N

N

V

V

V

T

G

G

N

D

A

L

V

T

A

G

S

T

V

A

I

I

V

V

A

A

K

K

W

T

E

E

binding [(2~{R})-1-[2-azanylethoxy(oxidanyl)phosphoryl]oxy-3-hexadecanoyloxy-propan-2-yl] (~{Z})-octadec-9-enoate: Y4 (≠ R4), G66 (= G66), V83 (≠ I84), I157 (≠ F161), Y164 (vs. gap), K193 (≠ Q189), T305 (≠ S301), I306 (≠ F302), I347 (≠ K343)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I13 (≠ M13), M199 (= M195), S275 (= S271), T311 (≠ S307), G356 (≠ S352), L384 (= L374), D391 (= D381), R394 (= R384)

2nwwA Crystal structure of gltph in complex with tboa (see paper)
34% identity, 94% coverage: 9:403/421 of query aligns to 7:407/407 of 2nwwA

query
sites
2nwwA

I

I

L

L

G

I

A

G

M

L

I

I

A

L

G

G

I

A

V

I

V

V

G

G

I

L

T

I

L

L

N

G

R

H

T

Y

I

G

T

Y

D

A

P

H

A

A

T

V

L

H

T

T

D

-

V

-

T

-

G

-

H

Y

I

V

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

L

A

T

S

S

L

A

M

F

S

A

L

V

T

T

L

L

G

G

L

I

I

I

M

M

V

A

N

R

L

L

L

F

Q

N

P

P

G

G

V

A

G

G

A

I

D

H

L

L

V

A

L

V

P

-

E

G

G

G

A

Q

S

Q

A

F

G

Q

V

P

S

H

Q

Q

A

A

D

P

F

-

S

P

L

L

K

V

Q

H

F

I

V

L

T

L

H

D

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

G

A

A

M

L

A

A

T

N

N

G

E

Q

I

V

L

L

Q

P

I

T

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

A

K

V

V

G

R

E

K

R

S

A

A

A

E

P

T

L

L

V

L

R

D

G

A

I

I

E

N

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q

K

I

I

T

V

D

N

Y

G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

H

I

V

L

V

L

G

S

E

F

L

G

A

K

K

F

V

M

T

G

A

S

A

F

V

Y

Y

L

V

S

G

M

L

F

T

L

L

L

Q

W

I

G

L

L

L

L

V

L

Y

L

F

L

V

C

L

Y

L

L

K

I

I

V

Y

G

G

G

I

R

D

S

P

K

I

L

S

L

F

I

I

R

K

Y

H

I

A

R

K

E

D

P

A

I

M

L

L

L

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

A

V

L

A

D

K

R

E

F

M

G

G

V

I

P

S

P

E

R

G

I

I

A

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

C

T

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

T

T

F

V

A

G

H

Q

Q

Q

V

L

T

T

M

I

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A

A

G

G

V

V

P

P

R

G

A
|
A

S
x
G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

H

F

S

F

V

D

G

I

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

E

A

A

A

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
|
R

S
x
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

A

L

V

T

A

G

S

T

V

A

I

I

V

V

A

A

K

K

W

T

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S269 (= S271), T305 (≠ S307), A349 (= A351), G350 (≠ S352), D385 (= D381), R388 (= R384), T389 (≠ S385), N392 (= N388)

6bavA Crystal structure of gltph r397c in complex with s-benzyl-l-cysteine (see paper)
33% identity, 95% coverage: 7:404/421 of query aligns to 2:409/409 of 6bavA

query
sites
6bavA

Y

Y

Y

P

I

V

L

L

G

Q

A

K

M

I

I

L

A

I

G

G

I

L

V

I

V

L

G

G

I

A

T

I

L

V

N

G

R

L

T

I

I

L

T

G

D

H

P

Y

A

G

T

Y

L

A

T

D

D

A

V

V

T

K

G

T

H

Y

I

V

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

L

A

T

S

S

L

A

M

F

S

A

L

V

T

T

L

L

G

G

L

I

I

I

M

M

V

A

N

R

L

L

L

F

Q

N

P

P

G

G

V

A

G

G

A

I

D

H

L

L

V

A

L

V

P

-

E

G

G

G

A

Q

S

Q

A

F

G

Q

V

P

S

K

Q

Q

A

A

D

P

F

-

S

P

L

L

K

V

Q

K

F

I

V

L

T

L

H

D

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

G

A

A

M

L

A

A

T

N

N

G

E

Q

I

V

L

L

Q

P

I

T

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

A

K

V

V

G

R

E

K

R

S

A

A

A

E

P

T

L

L

V

L

R

D

G

A

I

I

E

N

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q

K

I

I

T

V

D

N

Y

G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

K

I

V

L

V

L

G

S

E

F

L

G

A

K

K

F

V

M

T

G

A

S

A

F

V

Y

Y

L

V

S

G

M

L

F

T

L

L

L

Q

W

I

G

L

L

L

L

V

L

Y

L

F

L

V

C

L

Y

L

L

K

I

I

V

Y

G

G

G

I

R

D

S

P

K

I

L

S

L

F

I

I

R

K

Y

K

I

A

R

K

E

D

P

A

I

M

L

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

A

V

L

A

D

K

R

E

F

M

G

G

V

I

P

S

P

E

R

G

I

I

A

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

I

L

Y
|
Y

C

Q

T

G

F

V

A

C

T

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

T

T

F

V

A

G

H

Q

Q

Q

V

L

T

T

M

I

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A

A

G

G

V

V

P

P

R

G

A

A

S

G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

E

F

S

F

V

D

G

I

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

E

A

A

A

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D
|
D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
x
C

S
x
T

A

M

T

V

N

N

V

V

V

T

G

G

N

D

A

L

V

T

A

G

S

T

V

A

I

I

V

V

A

A

K

K

W

T

E

E

G

G

binding benzylcysteine: R268 (≠ A269), S270 (= S271), T306 (≠ S307), Y309 (= Y310), D382 (= D377), D386 (= D381), C389 (≠ R384), T390 (≠ S385)

6bauA Crystal structure of gltph r397c in complex with l-cysteine (see paper)
32% identity, 94% coverage: 7:403/421 of query aligns to 2:408/408 of 6bauA

query
sites
6bauA

Y

Y

Y

P

I

V

L

L

G

Q

A

K

M

I

I

L

A

I

G

G

I

L

V

I

V

L

G

G

I

A

T

I

L

V

N

G

R

L

T

I

I

L

T

G

D

H

P

Y

A

G

T

Y

L

A

T

D

D

A

V

V

T

K

G

T

H

Y

I

V

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

L

A

T

S

S

L

A

M

F

S

A

L

V

T

T

L

L

G

G

L

I

I

I

M

M

V

A

N

R

L

L

L

F

Q

N

P

P

G

G

V

A

G

G

A

I

D

H

L

L

V

A

L

V

P

-

E

G

G

G

A

Q

S

Q

A

F

G

Q

V

P

S

K

Q

Q

A

A

D

P

F

-

S

P

L

L

K

V

Q

K

F

I

V

L

T

L

H

D

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

G

A

A

M

L

A

A

T

N

N

G

E

Q

I

V

L

L

Q

P

I

T

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

A

K

V

V

G

R

E

K

R

S

A

A

A

E

P

T

L

L

V

L

R

D

G

A

I

I

E

N

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q

K

I

I

T

V

D

N

Y

G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

K

I

V

L

V

L

G

S

E

F

L

G

A

K

K

F

V

M

T

G

A

S

A

F

V

Y

Y

L

V

S

G

M

L

F

T

L

L

L

Q

W

I

G

L

L

L

L

V

L

Y

L

F

L

V

C

L

Y

L

L

K

I

I

V

Y

G

G

G

I

R

D

S

P

K

I

L

S

L

F

I

I

R

K

Y

K

I

A

R

K

E

D

P

A

I

M

L

L

L

T

A

A

F

F

S

V

T

T

A

R

S

S

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

A

V

L

A

D

K

R

E

F

M

G

G

V

I

P

S

P

E

R

G

I

I

A

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

C

T

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

T

T

F

V

A

G

H

Q

Q

Q

V

L

T

T

M

I

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A
|
A

G
|
G

V
|
V

P

P

R

G

A

A

S
x
G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

E

F

S

F

V

D

G

I

L

P

P

-

L

-

T

-

D

-

P

-

N

-

V

E

A

A

A

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
x
C

S
x
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

A

L

V

T

A

G

S

T

V

A

I

I

V

V

A

A

K

K

W

T

E

E

binding cysteine: S270 (= S271), M303 (≠ L304), T306 (≠ S307), A345 (= A346), G346 (= G347), V347 (= V348), G351 (≠ S352), D386 (= D381), C389 (≠ R384), T390 (≠ S385), N393 (= N388)

5e9sA Crystal structure of substrate-bound glutamate transporter homologue glttk (see paper)
32% identity, 97% coverage: 3:409/421 of query aligns to 4:421/427 of 5e9sA

query
sites
5e9sA

K

R

R

Y

L

L

T

D

Y

Y

Y

P

I

V

L

L

G

W

A

K

M

I

I

L

A

W

G

G

I

L

V

V

V

L

G

G

I

A

T

V

L

F

N

G

R

L

T

I

I

A

T

G

D

H

P

F

A

G

T

Y

L

A

T

G

D

A

V

V

T

K

G

T

H

Y

I

I

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F
x
Y

L

L

T

A

A
x
T

S
|
S

L

A

M

M

S

A

L

V

T

F

L

F

G

G

L

L

I

I

M

V

V

G

N

R

L

L

L

F

Q

N

P

V

G

G

V

A

G

N

A

V

D

N

L

L

V

-

L

-

P

-

P

G

E

S

G

G

A

T

S

G

A

K

G

A

V

I

S

E

Q

A

A

Q

D

P

F

P

S

S

L

L

K

V

Q

Q

F

T

V

L

T

L

H

N

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

A

A

S

M

L

A

A

T

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

A

Y

V

L

G

M

E

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

A

E

P

T

L

L

V

L

R

R

G

V

I

F

E

D

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q
x
L

I

I

T

V

D

G

Y
x
G

V

V

M

M

R

Q

F
x
Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

R

I

V

L

V

L

G

S

P

F

L

G

A

K

K

F

V

M

V

G

G

S

A

F

V

Y

Y

L

T

S

G

M

L

F

F

L

L

L

Q

W

I

G

V

L

I

L

T

L

Y

L

F

L

I

C

L

Y

L

L

K

I

V

V

F

G

G

G

I

R

D

S

P

K

I

L

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

-

V

A

A

L

E

D

E

R

E

F

M

G

G

V

V

P

D

P

K

R

G

I

I

A

F

S

S

F

F

V

T

L

L

P

P

L

L

G
|
G

Y
x
A

S
x
T

F

I

N
|
N

L
x
M

D
|
D

G

G

S
x
T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

T

T

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

G

G

I

H

E

P

L

L

T

T

F

L

A

G

H

Q

Q

Q

V

L

T

V

M

V

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S
|
S

K
x
I

G
|
G

I
x
T

A

A

G
|
G

V
|
V

P

P

R

G

A
|
A

S
x
G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

Q

F

S

F

V

D

G

I

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

E

V

A

A

-

L

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
|
R

S
x
T

A

M

T

V

N
|
N

V
|
V

V

T

G

G

N
x
D

A

L

V

A

A

G

S

T

V

V

I

I

V

V

A

A

K

K

W

T

E

E

G

-

G

K

R

E

L

L

D

D

E

E

binding aspartic acid: R274 (≠ A269), S275 (= S270), S276 (= S271), T313 (≠ S307), G353 (= G347), V354 (= V348), A357 (= A351), G358 (≠ S352), D394 (= D381), R397 (= R384), T398 (≠ S385)
binding decyl-beta-d-maltopyranoside: L194 (≠ Q189), G198 (≠ Y193), Y202 (≠ F197)
binding sodium ion: Y87 (≠ F87), T90 (≠ A90), S91 (= S91), S276 (= S271), G305 (= G299), A306 (≠ Y300), T307 (≠ S301), N309 (= N303), N309 (= N303), M310 (≠ L304), D311 (= D305), S348 (= S342), I349 (≠ K343), G350 (= G344), T351 (≠ I345), N401 (= N388), V402 (= V389), D405 (≠ N392)

6xwnB Structure of glutamate transporter homologue glttk in the presence of tboa inhibitor (see paper)
32% identity, 97% coverage: 3:409/421 of query aligns to 4:421/426 of 6xwnB

query
sites
6xwnB

K

R

R

Y

L

L

T

D

Y

Y

Y

P

I

V

L

L

G

W

A

K

M

I

I

L

A

W

G

G

I

L

V

V

V

L

G

G

I

A

T

V

L

F

N

G

R

L

T

I

I

A

T

G

D

H

P

F

A

G

T

Y

L

A

T

G

D

A

V

V

T

K

G

T

H

Y

I

I

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

A

A

T

S

S

L

A

M

M

S

A

L

V

T

F

L

F

G

G

L

L

I

I

M

V

V

G

N

R

L

L

L

F

Q

N

P

V

G

G

V

A

G

N

A

V

D

N

L

L

V

-

L

-

P

-

P

G

E

S

G

G

A

T

S

G

A

K

G

A

V

I

S

E

Q

A

A

Q

D

P

F

P

S

S

L

L

K

V

Q

Q

F

T

V

L

T

L

H

N

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

A

A

S

M

L

A

A

T

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

A

Y

V

L

G

M

E

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

A

E

P

T

L

L

V

L

R

R

G

V

I

F

E

D

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q

L

I

I

T

V

D

G

Y

G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

R

I

V

L

V

L

G

S

P

F

L

G

A

K

K

F

V

M

V

G

G

S

A

F

V

Y

Y

L

T

S

G

M

L

F

F

L

L

L

Q

W

I

G

V

L

I

L

T

L

Y

L

F

L

I

C

L

Y

L

L

K

I

V

V

F

G

G

G

I

R

D

S

P

K

I

L

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A

R

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

-

V

A

A

L

E

D

E

R

E

F

M

G

G

V

V

P

D

P

K

R

G

I

I

A

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

T

T

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

G

G

I

H

E

P

L

L

T

T

F

L

A

G

H

Q

Q

Q

V

L

T

V

M

V

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A

A

G

G

V

V

P

P

R

G

A

A

S

G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

Q

F

S

F

V

D

G

I

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

E

V

A

A

-

L

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
|
R

S

T

A

M

T

V

N

N

V

V

V

T

G

G

N

D

A

L

V

A

A

G

S

T

V

V

I

I

V

V

A

A

K

K

W

T

E

E

G

-

G

K

R

E

L

L

D

D

E

E

binding (3s)-3-(benzyloxy)-l-aspartic acid: S275 (= S270), S276 (= S271), M310 (≠ L304), T313 (≠ S307), D394 (= D381), R397 (= R384)

6zgbA Glutamate transporter homologue glttk in complex with a photo cage compound (see paper)
32% identity, 97% coverage: 3:409/421 of query aligns to 2:419/425 of 6zgbA

query
sites
6zgbA

K

R

R

Y

L

L

T

D

Y

Y

Y

P

I

V

L

L

G

W

A

K

M

I

I

L

A

W

G

G

I

L

V

V

V

L

G

G

I

A

T

V

L

F

N

G

R

L

T

I

I

A

T

G

D

H

P

F

A

G

T

Y

L

A

T

G

D

A

V

V

T

K

G

T

H

Y

I

I

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

A

A

T

S

S

L

A

M

M

S

A

L

V

T

F

L

F

G

G

L

L

I

I

M

V

V

G

N

R

L

L

L

F

Q

N

P

V

G

G

V

A

G

N

A

V

D

N

L

L

V

-

L

-

P

-

P

G

E

S

G

G

A

T

S

G

A

K

G

A

V

I

S

E

Q

A

A

Q

D

P

F

P

S

S

L

L

K

V

Q

Q

F

T

V

L

T

L

H

N

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

A

A

S

M

L

A

A

T

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

A

Y

V

L

G

M

E

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

A

E

P

T

L

L

V

L

R

R

G

V

I

F

E

D

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q

L

I

I

T

V

D

G

Y

G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

R

I

V

L

V

L

G

S

P

F

L

G

A

K

K

F

V

M

V

G

G

S

A

F

V

Y

Y

L

T

S

G

M

L

F

F

L

L

L

Q

W

I

G

V

L

I

L

T

L

Y

L

F

L

I

C

L

Y

L

L

K

I

V

V

F

G

G

G

I

R

D

S

P

K

I

L

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

-

V

A

A

L

E

D

E

R

E

F

M

G

G

V

V

P

D

P

K

R

G

I

I

A

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S

T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

T

T

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

G

G

I

H

E

P

L

L

T

T

F

L

A

G

H

Q

Q

Q

V

L

T

V

M

V

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A

A

G

G

V

V

P

P

R

G

A

A

S

G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

Q

F

S

F

V

D

G

I

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

E

V

A

A

-

L

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
|
R

S
x
T

A

M

T

V

N

N

V

V

V

T

G

G

N

D

A

L

V

A

A

G

S

T

V

V

I

I

V

V

A

A

K

K

W

T

E

E

G

-

G

K

R

E

L

L

D

D

E

E

binding (2~{S},3~{S})-2-azanyl-3-[(2-nitrophenyl)methoxy]butanedioic acid: R272 (≠ A269), S273 (= S270), S274 (= S271), M308 (≠ L304), D392 (= D381), R395 (= R384), T396 (≠ S385)

6zl4A The structure of glutamate transporter homologue glttk in complex with the photo switchable compound (cis) (see paper)
32% identity, 97% coverage: 3:409/421 of query aligns to 1:418/424 of 6zl4A

query
sites
6zl4A

K

R

R

Y

L
|
L

T

D

Y

Y

Y

P

I

V

L

L

G

W

A

K

M

I

I

L

A

W

G

G

I

L

V

V

V

L

G

G

I

A

T

V

L

F

N

G

R

L

T

I

I

A

T

G

D

H

P

F

A

G

T

Y

L

A

T

G

D

A

V

V

T

K

G

T

H

Y

I

I

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

A

A

T

S

S

L

A

M

M

S

A

L

V

T

F

L

F

G

G

L

L

I

I

M

V

V

G

N

R

L

L

L

F

Q

N

P

V

G

G

V

A

G

N

A

V

D

N

L

L

V

-

L

-

P

-

P

G

E

S

G

G

A

T

S

G

A

K

G

A

V

I

S

E

Q

A

A

Q

D

P

F

P

S

S

L

L

K

V

Q

Q

F

T

V

L

T

L

H

N

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

A

A

S

M

L

A

A

T

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

A

Y

V

L

G

M

E

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

A

E

P

T

L

L

V

L

R

R

G

V

I

F

E

D

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q
x
L

I

I

T

V

D

G

Y
x
G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

R

I

V

L

V

L

G

S

P

F

L

G

A

K

K

F

V

M

V

G

G

S

A

F

V

Y

Y

L

T

S

G

M

L

F

F

L

L

L

Q

W

I

G

V

L

I

L

T

L

Y

L

F

L

I

C

L

Y

L

L

K

I

V

V

F

G

G

G

I

R

D

S

P

K

I

L

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E
x
R

-

V

A

A

L

E

D

E

R

E

F

M

G

G

V

V

P

D

P

K

R

G

I

I

A

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

T

T

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

G

G

I

H

E

P

L

L

T

T

F

L

A

G

H

Q

Q

Q

V

L

T

V

M

V

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I

T

A

A

G

G

V

V

P

P

R
x
G

A
|
A

S

G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

Q

F

S

F

V

D

G

I

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

E

V

A

A

-

L

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D

D

M

M

G

G

R
|
R

S
x
T

A

M

T

V

N

N

V

V

V

T

G

G

N

D

A

L

V

A

A

G

S

T

V

V

I

I

V

V

A

A

K

K

W

T

E

E

G

-

G

K

R

E

L

L

D

D

E

E

binding decyl-beta-d-maltopyranoside: L3 (= L5), L191 (≠ Q189), G195 (≠ Y193), R282 (≠ E280)
binding (2~{S},3~{S})-2-azanyl-3-[[4-[2-(4-methoxyphenyl)hydrazinyl]phenyl]methoxy]butanedioic acid: R271 (≠ A269), S272 (= S270), S273 (= S271), M307 (≠ L304), T310 (≠ S307), G353 (≠ R350), A354 (= A351), R394 (= R384), T395 (≠ S385)

6r7rA Crystal structure of the glutamate transporter homologue glttk in complex with d-aspartate (see paper)
32% identity, 96% coverage: 7:409/421 of query aligns to 1:410/416 of 6r7rA

query
sites
6r7rA

Y

Y

Y

P

I

V

L

L

G

W

A

K

M

I

I

L

A

W

G

G

I

L

V

V

V

L

G

G

I

A

T

V

L

F

N

G

R

L

T

I

I

A

T

G

D
x
H

P

F

A

G

T

Y

L

A

T

G

D

A

V

V

T

K

G

T

H

Y

I

I

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

L

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

A

A

T

S

S

L

A

M

M

S

A

L

V

T

F

L

F

G

G

L

L

I

I

M

V

V

G

N

R

L

L

L

F

Q

N

P

V

G

G

V

A

G

N

A

V

D

N

L

L

V

-

L

-

P

-

E

-

G

-

A

-

S

G

A

S

G

G

V

T

S

G

Q

A

A

Q

D

P

F

P

S

S

L

L

K

V

Q

Q

F

T

V

L

T

L

H

N

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

A

A

S

M

L

A

A

T

K

N

G

E

E

I

V

L

L

Q

P

I

V

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

A

Y

V

L

G

M

E

N

R

R

-

N

-

E

-

R

-

V

-

R

-

K

-

S

A

A

A

E

P

T

L

L

V

L

R

R

G

V

I

F

E

D

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q

L

I

I

T

V

D

G

Y

G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

R

I

V

L
x
V

L

G

S

P

F

L

G
x
A

K

K

F

V

M

V

G

G

S

A

F

V

Y

Y

L

T

S

G

M

L

F

F

L

L

L

Q

W

I

G

V

L

I

L

T

L

Y

L

F

L

I

C

L

Y

L

L

K

I

V

V

F

G

G

G

I

R

D

S

P

K

I

L

K

L

F

I

I

R

R

Y

K

I

A

R

K

E

D

P

A

I

M

L

I

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

-

V

A

A

L

E

D

E

R

E

F

M

G

G

V

V

P

D

P

K

R

G

I

I

A

F

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

T

T

V

M

L

F

F

I

V

A

A

Q

N

A

A

Y

I

G

G

I

H

E

P

L

L

T

T

F

L

A

G

H

Q

Q

Q

V

L

T

V

M

V

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

S

K

I

G

G

I
x
T

A

A

G
|
G

V
|
V

P

P

R

G

A

A

S
x
G

L

A

V

I

I

M

I

L

S

A

S

M

T

V

L

L

A

Q

F

S

F

V

D

G

I

L

-

D

-

L

-

T

-

P

-

G

-

S

P

P

E

V

A

A

-

L

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R
|
R

S
x
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

A

L

V

A

A

G

S

T

V

V

I

I

V

V

A

A

K

K

W

T

E

E

G

-

G

K

R

E

L

L

D

D

E

E

binding d-aspartic acid: R263 (≠ A269), S265 (= S271), M299 (≠ L304), T302 (≠ S307), T340 (≠ I345), G342 (= G347), V343 (= V348), G347 (≠ S352), D383 (= D381), R386 (= R384), T387 (≠ S385), N390 (= N388)
binding decyl-beta-d-maltopyranoside: H23 (≠ D29), V212 (≠ L218), A216 (≠ G222)

6bmiA Crystal structure of gltph r397c in complex with l-serine (see paper)
32% identity, 94% coverage: 7:403/421 of query aligns to 2:396/396 of 6bmiA

query
sites
6bmiA

Y

Y

Y

P

I

V

L

L

G

Q

A

K

M

I

I

L

A

I

G

G

I

L

V

I

V

L

G

G

I

A

T

I

L

V

N

G

R

L

T

I

I

L

T

G

D

H

P

Y

A

G

T

Y

L

A

T

D

D

A

V

V

T

K

G

T

H

Y

I

V

S

K

I

P

L

F

T

G

E

D

L

L

F

F

L

V

R

R

L

L

I

L

K

K

M

M

I

L

I

V

A

M

P

P

L

I

V

V

F

F

A

A

T

S

L

L

V

V

T

V

G

G

I

A

A

A

H

S

M

I

G

-

D

S

T

P

A

A

A

R

L

L

G

G

R

R

V

V

G

G

F

V

R

K

S

I

I

V

A

V

W

Y

F

Y

L

L

T

L

A

T

S

S

L

A

M

F

S

A

L

V

T

T

L

L

G

G

L

I

I

I

M

M

V

A

N

R

L

L

L

F

Q

N

P

P

G

G

V

A

G

G

A

I

D

H

L

L

V

A

L

V

P

-

E

G

G

G

A

Q

S

Q

A

F

G

Q

V

P

S

K

Q

Q

A

A

D

P

F

-

S

P

L

L

K

V

Q

K

F

I

V

L

T

L

H

D

L

I

V

V

P

P

K

T

S

N

F

P

F

F

E

G

A

A

M

L

A

A

T

N

N

G

E

Q

I

V

L

L

Q

P

I

T

V

I

V

F

F

F

S

A

V

I

F

I

T

L

G

G

V

I

A

A

I

I

T

T

-

Y

-

L

-

M

-

N

-

S

-

E

-

N

-

E

A

K

V

V

G

R

E

K

R

S

A

A

A

E

P

T

L

L

V

L

R

D

G

A

I

I

E

N

A

G

L

L

V

A

Q

E

V

A

M

M

L

Y

Q

K

I

I

T

V

D

N

Y

G

V

V

M

M

R

Q

F

Y

A

A

P

P

F

I

A

G

V

V

F

F

A

A

A

L

V

I

T

A

T

Y

A

V

L

M

A

A

E

E

Q

Q

G

G

P

V

G

K

I

V

L

V

L

G

S

E

F

L

G

A

K

K

F

V

M

T

G

A

S

A

F

V

Y

Y

L

V

S

G

M

L

F

T

L

L

L

Q

W

I

G

L

L

L

L

V

L

Y

L

F

L

V

C

L

Y

L

L

K

I

I

V

Y

G

G

G

I

R

D

S

P

K

I

L

S

L

F

I

I

R

K

Y

K

I

A

R

K

E

D

P

A

I

M

L

L

L

T

A

A

F

F

S

V

T

T

A
x
R

S

S

S
|
S

E

S

A

G

A

T

F

L

P

P

R

V

T

T

L

M

E

R

A

V

L

A

D

K

R

E

F

M

G

G

V

I

P

S

P

E

R

G

I

I

A

Y

S

S

F

F

V

T

L

L

P

P

L

L

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

Q

T

G

F

V

A

C

T

T

M

F

F

F

I

I

A

A

Q

N

A

A

Y

L

G

G

I

S

E

H

L

L

T

T

F

V

A

G

H

Q

Q

Q

V

L

T

T

M

I

L

V

L

L

I

T

L

A

M

V

V

L

T

A

S

I

K

-

G

-

I

-

A

-

G

-

V

-

P

-

R

M

A

L

S

A

L

M

V

V

I

L

I

E

S

S

S

V

T

G

L

L

A

P

F

L

F

T

D

D

I

-

P

P

E

N

-

V

-

A

A

A

G

A

L

Y

L

A

L

M

I

I

L

L

A

G

V

I

D

D

H

A

F

I

L

L

D
|
D

M

M

G

G

R

C

S
x
T

A

M

T

V

N
|
N

V

V

V

T

G

G

N

D

A

L

V

T

A

G

S

T

V

A

I

I

V

V

A

A

K

K

W

T

E

E

binding serine: R268 (≠ A269), S270 (= S271), T306 (≠ S307), D374 (= D381), T378 (≠ S385), N381 (= N388)

7awmA Structure of the thermostabilized eaat1 cryst mutant in complex with l-asp, three sodium ions and the allosteric inhibitor ucph101 (see paper)
30% identity, 88% coverage: 45:414/421 of query aligns to 58:412/412 of 7awmA

query
sites
7awmA

E

E

L

L

F

L

L

M

R

R

L

M

I

L

K

K

M

M

I

L

I

I

A

L

P

P

L

L

V

I

F

V

A

S

T

S

L

L

V

I

T

T

G

G

I

L

A

A

H

S

M

L

G

-

D

D

T

A

A

K

A

A

L
x
S

G
|
G

R

R

V

L

G
|
G

F

M

R

R

S
x
A

I
x
V

A

V

W

Y

F
x
Y

L

M

T

S

A

T

S

T

L

I

M

I

S

A

L

V

T

V

L

L

G

G

L

I

I

I

M

L

V

V

N

L

L

I

Q

H

P

P

G

E

V

V

G

-

A

L

D

D

L

C

V

F

L

L

P

-

E

-

G

-

A

-

S

-

A

-

G

-

V

-

S

-

Q

-

A

-

D

D

F

L

S

A

L

R

K

N

Q

I

F

F

V

P

T

S

H

N

L

L

V

V

P

S

K

A

S

A

F

F

F

-

E

R

A

S

M

Y

A

S

T

T

N

Q

E

E

-

V

-

E

-

G

-

M

-

N

I

I

L

L

Q

G

I

L

V

V

F

F

S

S

V
x
M

F

V

T

F

G

G

V
x
F

A

A

I

L

T

G

A

K

V

M

G

G

E

E

R

Q

A

G

A

Q

P

L

L

L

V

V

R

D

G

F

I

F

E

N

A

S

L

L

V

N

Q

E

V

A

M

T

L

M

Q

K

I

L

T

V

D

A

Y

I

V

I

M

M

R

W

F

Y

A

A

P

P

F

L

A

G

V

I

F

L

A

F

A

L

V

I

T

A

T

G

A

K

L

I

A

V

E

G

Q

G

G

-

P

-

G

-

I

-

L

-

S

Q

F

L

G

G

K

M

F

Y

M

M

G

V

S

T

F

V

Y

I

L

V

S

G

M

L

F

V

L

I

L

H

W

G

G

L

L

I

L

V

L

L

L

P

L

L

C

I

Y

Y

L

F

I

L

V

I

G

T

G

R

R

K

S

N

K

P

L

F

L

V

-

F

I

I

R

A

Y

G

I

I

R

L

E

Q

P

A

I

L

L

I

L

T

A

A

F

L

S

G

T

T

A

S

S
|
S

E

S

A

A

A

T

F

L

P

P

R

I

T

T

L

F

E

K

A

C

L

L

D

E

-

E

R

N

F

N

G

G

V

V

P

D

P

K

R

R

I

I

A

T

S

R

F
|
F

V

V

L

L

P

P

L
x
V

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

E

T

A

F

V

A

A

T

A

M

I

F

F

I

I

A

A

Q

Q

A

V

Y

N

G

N

I

Y

E

E

L

L

T

D

F

F

A

G

H

Q

Q

I

V

I

T

T

M

I

L

S

L

I

I

T

L

A

M

T

V

A

T

A

S

S

K

I

G

G

I

A

A

A

G
|
G

V
x
I

P

P

R

Q

A
|
A

S
x
G

L

L

V

V

I

T

I

M

S

V

S

I

T

V

L

L

A

T

F

A

F

V

D

G

I

L

P

P

E

T

A

D

G

D

L

I

L

T

L

L

I

I

L

I

A

A

V

V

D

D

H

W

F

L

L

L

D
|
D

M

R

G

F

R
|
R

S
x
T

A

M

T

V

N

N

V

V

V

L

G

G

N

D

A

A

V

L

A

G

S

A

V

G

I

I

V

V

A

E

K

H

W

L

E

S

G

R

G

K

R

E

L

L

D

E

E

K

I

-

E

Q

P

D

A

A

E

E

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: S88 (≠ L76), G89 (= G77), G92 (= G80), A95 (≠ S83), V96 (≠ I84), Y99 (≠ F87), M163 (≠ V160), F167 (≠ V164), F293 (= F294), V297 (≠ L298)
binding aspartic acid: S268 (= S270), S269 (= S271), T306 (≠ S307), G346 (= G347), I347 (≠ V348), A350 (= A351), G351 (≠ S352), D380 (= D381), R383 (= R384), T384 (≠ S385)

5mjuA Structure of the thermostabilized eaat1 cryst mutant in complex with the competititve inhibitor tfb-tboa and the allosteric inhibitor ucph101 (see paper)
28% identity, 87% coverage: 45:409/421 of query aligns to 50:394/397 of 5mjuA

query
sites
5mjuA

E

E

L

L

F

L

L

M

R

R

L

M

I

L

K

K

M

M

I

L

I

I

A

L

P

P

L

L

V
x
I

F

V

A

S

T

S

L

L

V

I

T

T

G

G

I
x
L

A

A

H

S

M

L

G

-

D

D

T

A

A

K

A

A

L
x
S

G
|
G

R

R

V

L

G
|
G

F

M

R

R

S

A

I

V

A

V

W

Y

F
x
Y

L

M

T

S

A

T

S

T

L

I

M

I

S

A

L

V

T

V

L

L

G

G

L

I

I

I

M

L

V

V

N

L

L

I

Q

H

P

E

G

V

V

L

G

D

A

C

D

F

L

L

V

-

L

-

P

-

E

-

G

-

A

-

S

-

A

-

G

-

V

-

S

-

Q

-

A

-

D

D

F

L

S

A

L

R

K

N

Q

I

F

F

V

P

T

S

H

N

L

L

V

V

P

S

K

A

S

A

F

F

F

R

E

S

A

Y

M

S

A

T

T

T

N

G

E

Q

-

E

-

V

-

E

-

G

-

M

-

N

I
|
I

L

L

Q

G

I

L

V

V

F

F

S

S

V
x
M

F

V

T

F

G

G

V
x
F

A

A

I

L

T

G

A

K

V

M

G

G

E

E

R

Q

A

G

A

Q

P

L

L

L

V

V

R

D

G

F

I

F

E

N

A

S

L

L

V

N

Q

E

V

A

M

T

L

M

Q

K

I

L

T

V

D

A

Y

I

V

I

M

M

R

W

F

Y

A

A

P

P

F

L

A

G

V

I

F

L

A

F

A

L

V

I

T

A

T

G

A

K

L

I

A

V

E

E

Q

G

G

-

P

-

G

-

I

-

L

-

S

Q

F

L

G

G

K

M

F

Y

M

M

G

V

S

T

F

V

Y

I

L

V

S

G

M

L

F

V

L

I

L

H

W

G

G

L

L

I

L

V

L

L

L

P

L

L

C

I

Y

Y

L

F

I

L

V

I

G

T

G

R

R

K

S

N

K

P

L

F

L

V

-

F

I

I

R

A

Y

G

I

I

R

L

E

Q

P

A

I

L

L

I

L

T

A

A

F

L

S

G

T

T

A

S

S

S

S
|
S

E
x
S

A

A

A

T

F

L

P

P

R

I

T

T

L

F

E

K

A

C

L

L

D

E

-

E

R

N

F

N

G

G

V

V

P

D

P

K

R

R

I

I

A

T

S

R

F
|
F

V

V

L

L

P

P

L
x
V

G

G

Y
x
A

S
x
T

F

I

N

N

L
x
M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

E

T

A

F

V

A

A

T

A

M

I

F

F

I

I

A

A

Q

Q

A

V

Y

N

G

Q

I

I

E

-

L

-

T

-

F

-

A

-

H

-

Q

-

V

I

T

T

M

I

L

S

L

I

I

T

L

A

M

T

V

A

T

A

S

S

K

I

G
|
G

I

A

A

A

G

G

V

I

P

P

R

Q

A
|
A

S
x
G

L

L

V

V

I

T

I

M

S

V

S

I

T

V

L

L

A

T

F

A

F

V

D

G

I

L

P

P

E

T

A

D

G

D

L

I

L

T

L

L

I

I

L

I

A

A

V

V

D

D

H

W

F

L

L

L

D
|
D

M

R

G

F

R
|
R

S

T

A

M

T

V

N
|
N

V

V

V

L

G

G

N

D

A

A

V

L

A

G

S

A

V

G

I

I

V

V

A

E

K

H

W

L

E

S

G

R

G

K

R

E

L

L

D

E

E

K

binding 2-Amino-5,6,7,8-tetrahydro-4-(4-methoxyphenyl)-7-(naphthalen-1-yl)-5-oxo-4H-chromene-3-carbonitrile: L72 (≠ I67), S80 (≠ L76), G81 (= G77), G84 (= G80), Y91 (≠ F87), M156 (≠ V160), F160 (≠ V164), F286 (= F294), V290 (≠ L298)
binding (2~{S},3~{S})-2-azanyl-3-[[3-[[4-(trifluoromethyl)phenyl]carbonylamino]phenyl]methoxy]butanedioic acid: I64 (≠ V59), I148 (= I152), S262 (= S271), S263 (≠ E272), A292 (≠ Y300), T293 (≠ S301), M296 (≠ L304), T299 (≠ S307), G329 (= G344), A336 (= A351), G337 (≠ S352), D366 (= D381), R369 (= R384), N373 (= N388)

P43003 Excitatory amino acid transporter 1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Homo sapiens (Human) (see 3 papers)
27% identity, 92% coverage: 12:399/421 of query aligns to 57:494/542 of P43003

query
sites
P43003

A

A

M

V

I

I

A

V

G

G

I

T

V

I

V

L

G

G

I

F

T

T

L

L

N

R

R

-

T

-

I

-

T

-

D

-

P

P

A

Y

T

R

L

M

T

S

-

Y

D

R

V

E

T

V

G

K

H

Y

I

F

S

S

I

F

L

P

T

G

E

E

L

L

F

L

L

M

R

R

L

M

I

L

K

Q

M

M

I

L

I

V

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

L

V

V

T

T

G

G

I

M

A

A

H

A

M

L

G

-

D

D

T

S

A

K

A

A

L

S

G

G

R

K

V

M

G

G

F

M

R

R

S

A

I

V

A

V

W

Y

F

Y

L

M

T

T

A

T

S

T

L

I

M

I

S

A

L

V

T

V

L

I

G

G

L

I

I

I

M

I

V

V

N

I

L

I

Q

H

P

P

G

G

V

K

G

G

A

T

D

K

L

E

V

N

L

M

P

H

P

R

E

E

G

G

A

K

S

I

A

V

G

R

V

V

S

T

Q

A

A

A

D

D

F

A

S

F

L

L

-

D

-

L

-

I

-

R

-

N

-

M

-

F

-

P

-

N

-

L

-

V

-

E

-

A

-

C

-

F

K

K

Q

Q

F

F

V

K

T

T

H

N

L

Y

V

E

P

K

K

R

S

S

F

F

F

K

E

V

A

P

M

I

A

Q

T

A

N

N

E

E

I

T

L

L

-

V

-

G

-

A

-

V

-

I

-

N

-

V

-

S

-

E

-

A

-

M

-

E

-

T

-

L

-

T

-

R

-

I

-

T

-

E

-

L

-

V

-

P

-

V

-

P

-

G

-

S

-

V

-

N

-

G

-

V

-

N

-

A

-

L

Q

G

I

L

V

V

F

F

S

S

V

M

F

C

T

F

G

G

V

F

A

V

I

I

T

G

A

N

V

M

G

K

E

E

R

Q

A

G

A

Q

P

A

L

L

V

R

R

E

G

F

I

F

E

D

A

S

L

L

V

N

Q

E

V

A

M

I

L

M

Q

R

I

L

T

V

D

A

Y

V

V

I

M

M

R

W

F

Y

A

A

P

P

F

V

A

G

V

I

F

L

A

F

A

L

V

I

T

A

T

G

A

K

L

I

A

V

E

E

Q

-

G

-

P

-

G

-

I

-

L

M

L

E

S

D

F

M

G

G

K

V

F

I

M

G

G

G

S

Q

F

L

Y

A

L

M

-

Y

S

T

M

V

F

T

L

V

L

I

W

V

G

G

L

L

L

I

-

H

-

A

-

V

-

I

-

V

-

L

-

P

L

L

C

Y

Y

F

L

L

I

V

V

T

G

R

G

K

R

N

S

P

K

W

L

V

L

F

I

I

R

G

Y

G

I

L

R

L

E

Q

P

A

I

L

L

I

L

T

A

A

F

L

S

G

T

T

A
x
S

S
|
S

E

S

A

A

A

T

F

L

P

P

R

I

T

T

L

F

E

K

A

C

L

L

D

E

-

E

R

N

F

N

G

G

V

V

P

D

P

K

R

R

I

V

A

T

S

R

F

F

V

V

L

L

P

P

L

V

G

G

Y

A

S
x
T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

E

T

A

F

L

A

A

T

A

M

I

F

F

I

I

A

A

Q

Q

A

V

Y

N

G

N

I

F

E

E

L

L

T

N

F

F

A

G

H

Q

Q

I

V

I

T

T

M

I

L

S

L

I

I

T

L

A

M

T

V

A

T

A

S

S

K

I

G

G

I

A

A

A

G

G

V
x
I

P
|
P

R
x
Q

A
|
A

S
x
G

L

L

V

V

I

T

I

M

S

V

S

I

T

V

L

L

A

T

F

S

F

V

D

G

I

L

P

P

E

T

A

D

G

D

L

I

L

T

L

L

I

I

L

I

A

A

V

V

D

D

H

W

F

F

L

L

D
|
D

M
x
R

G

L

R

R

S

T

A

T

T

T

N
|
N

V

V

V

L

G

G

N

D

A

S

V

L

A

G

S

A

V

G

I

I

V

V

S363 (≠ A269) mutation to R: Loss of electrogenic glutamate transport. Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with M-477.
SSS 363:365 (≠ ASS 269:271) binding L-aspartate
T396 (≠ S301) binding Na(+)
T402 (≠ S307) binding L-aspartate
IPQAG 443:447 (≠ VPRAS 348:352) binding L-aspartate
D476 (= D381) binding L-aspartate
R477 (≠ M382) mutation to M: Strongly decreased L-aspartate and L-glutamate uptake combined with strongly increased permeability ot other ions; when associated with R-363.
N483 (= N388) binding L-aspartate

Sites not aligning to the query:

523 Y→F: No effect on activity.

P56564 Excitatory amino acid transporter 1; Glial high affinity glutamate transporter; High-affinity neuronal glutamate transporter; GluT-1; Sodium-dependent glutamate/aspartate transporter 1; GLAST-1; Solute carrier family 1 member 3 from Mus musculus (Mouse) (see paper)
27% identity, 84% coverage: 45:399/421 of query aligns to 86:494/543 of P56564

query
sites
P56564

E

E

L

L

F

L

L

M

R

R

L

M

I

L

K

Q

M

M

I

L

I

V

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

L

V

V

T

T

G

G

I

M

A

A

H

A

M

L

G

-

D

D

T

S

A

K

A

A

L

S

G

G

R

K

V

M

G

G

F

M

R

R

S

A

I

V

A

V

W

Y

F

Y

L

M

T

T

A

T

S

T

L

I

M

I

S

A

L

V

T

V

L

I

G

G

L

I

I

I

M

I

V

V

N

I

L

I

Q

H

P

P

G

G

V

K

G

G

A

T

D

K

L

E

V

N

L

M

P

Y

P

R

E

E

G

G

A

K

S

I

A

V

G

Q

V

V

S

T

Q

A

A

A

D

D

F

A

S

F

L

L

-

D

-

L

-

I

-

R

-

N

-

M

-

F

-

P

-

N

-

L

-

V

-

E

-

A

-

C

-

F

K

K

Q

Q

F

F

V

K

T

T

H

S

L

Y

V

E

P

K

K

R

S

S

F

F

F

K

E

V

A

P

M

I

A

Q

T

S

N
|
N

E

E

I

T

L

L

-

L

-

G

-

A

-

V

-

I

-

N

-
x
N

-

V

-

S

-

E

-

A

-

M

-

E

-

T

-

L

-

T

-

R

-

I

-

R

-

E

-

M

-

V

-

P

-

V

-

P

-

G

-

S

-

V

-

N

-

G

-

V

-

N

-

A

-

L

Q

G

I

L

V

V

F

F

S

S

V

M

F

C

T

F

G

G

V

F

A

V

I

I

T

G

A

N

V

M

G

K

E

E

R

Q

A

G

A

Q

P

A

L

L

V

R

R

E

G

F

I

F

E

D

A

S

L

L

V

N

Q

E

V

A

M

I

L

M

Q

R

I

L

T

V

D

A

Y

V

V

I

M

M

R

W

F

Y

A

A

P

P

F

L

A

G

V

I

F

L

A

F

A

L

V

I

T

A

T

G

A

K

L

I

A

V

E

E

Q

-

G

-

P

-

G

-

I

-

L

M

L

E

S

D

F

M

G

G

K

V

F

I

M

G

G

G

S

Q

F

L

Y

A

L

M

-

Y

S

T

M

V

F

T

L

V

L

I

W

V

G

G

L

L

L

I

-

H

-

A

-

V

-

I

-

V

-

L

-

P

L

L

C

Y

Y

F

L

L

I

V

V

T

G

R

G

K

R

N

S

P

K

W

L

V

L

F

I

I

R

G

Y

G

I

L

R

L

E

Q

P

A

I

L

L

I

L

T

A

A

F

L

S

G

T

T

A

S

S

S

E

S

A

A

A

T

F

L

P

P

R

I

T

T

L

F

E

K

A

C

L

L

D

E

R

E

F

N

-

N

G

G

V

V

P

D

P

K

R

R

I

I

A

T

S

R

F

F

V

V

L

L

P

P

L

V

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

S

T

M

A

I

L

Y

Y

C

E

T

A

F

L

A

A

T

A

M

I

F

F

I

I

A

A

Q

Q

A

V

Y

N

G

N

I

F

E

D

L

L

T

N

F

F

A

G

H

Q

Q

I

V

I

T

T

M

I

L

S

L

I

I

T

L

A

M

T

V

A

T

A

S

S

K

I

G

G

I

A

A

A

G

G

V

I

P

P

R

Q

A

A

S

G

L

L

V

V

I

T

I

M

S

V

S

I

T

V

L

L

A

T

F

S

F

V

D

G

I

L

P

P

E

T

A

D

G

D

L

I

L

T

L

L

I

I

L

I

A

A

V

V

D

D

H

W

F

F

L

L

D

D

M

R

G

L

R

R

S

T

A

T

T

T

N

N

V

V

V

L

G

G

N

D

A

S

V

L

A

G

S

A

V

G

I

I

V

V

N206 (= N150) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N216 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine

P43007 Neutral amino acid transporter A; Alanine/serine/cysteine/threonine transporter 1; ASCT-1; Solute carrier family 1 member 4 from Homo sapiens (Human) (see 4 papers)
28% identity, 90% coverage: 45:421/421 of query aligns to 80:498/532 of P43007

query
sites
P43007

E

E

L

M

F

L

L

L

R

R

L

M

I

L

K

R

M

M

I

I

A

L

P

P

L

L

V

V

F

V

A

C

T

S

L

L

V

V

T

S

G

G

I

A

A

A

H

S

M

L

G

-

D

D

T

A

A

S

A

C

L

L

G

G

R

R

V

L

G

G

F

G

R

I

S

A

I

V

A

A

W

Y

F

F

L

G

T

L

A

T

S

T

L

L

M

S

S

A

L

S

T

A

L

L

G

A

L

V

I

A

M

L

V

A

N

F

L

I

Q

K

P

P

G

G

V

S

G

G

A

A

-

Q

-

T

-

L

-

Q

-

S

D

D

L

L

V

G

L

L

-

E

-

D

-

S

-

G

-

P

P

P

E

V

G

P

A

K

S

E

A

T

G

V

V

D

S

S

Q

F

A

L

D

D

F

L

S

A

L

R

K

N

Q

L

F

F

V

P

T

S

H

N

L

L

V

V

P

V

K

A

S

A

F

F

-

R

-

T

-

Y

-

A

-

T

-

D

F

Y

E

K

A

V

M

V

A

T

T

Q

N
|
N

-

S

-

G

-
x
N

-

V

-

T

-

H

-

E

-

K

-

I

-

P

-

I

-

G

-

T

-

E

-

I

-

E

-

G

-

M

E

N

I

I

L

L

Q

G

I

L

V

V

V

L

F

F

S

A

V

L

F

V

T

L

G

G

V

V

A

A

I

L

T

K

A

K

V

L

G

G

E

S

R

E

A

G

A

E

P

D

L

L

V

I

R

R

G

F

I

F

E

N

A

S

L

L

V

N

Q
x
E

V

A

M

T

L

M

Q

V

I

L

T

V

D

S

Y

W

V

I

M

M

R

W

F

Y

A

V

P

P

F

V

A

G

V

I

F

M

A

F

A

L

V

V

T

G

T

S

A

K

L

I

A

V

E

E

Q

M

G

K

P

D

G

I

I

I

L

V

L

L

-

V

-

T

S

S

F

L

G

G

K

K

F

Y

M

I

G

F

S

A

F

S

Y

I

L

L

S

G

M

H

F

V

L

I

L

H

W

G

G

G

L

I

L

V

L

L

L

P

L

L

C

I

Y

Y

L

F

I

V

V

F

G

T

G

R

R

K

S

N

K

P

L

F

-

R

L

F

I

L

R

L

Y

G

I

L

R

L

E

A

P

P

I

F

L

A

L

T

A

A

F

F

S

A

T

T

A

C

S

S

E

S

A

A

A

T

F

L

P

P

R

S

T

M

L

M

E

K

A

C

L

I

D

E

-

E

R

N

F

N

G

G

V

V

P

D

P

K

R

R

I

I

A

S

S

R

F

F

V

I

L

L

P

P

L

I

G

G

Y

A

S

T

F

V

N

N

L

M

D

D

G

G

S

A

M

A

I

I

Y

F

C

Q

T

C

F

V

A

A

T

A

M

V

F

F

I

I

A

A

Q

Q

A

L

Y

N

G

N

I

V

E

E

L

L

T

N

F

A

A

G

H

Q

Q

I

V

F

T

T

M

I

L

L

L

V

I

T

L

A

M

T

V

A

T

S

S

S

K

V

G

G

I

A

A

A

G

G

V

V

P

P

R

A

A

G

S

G

L

V

V

L

I

T

I

I

S

A

S

I

T

I

L

L

A

E

F

A

F

I

D

G

I

L

P

P

E

T

A

H

G

D

L

L

L

P

L

L

I

I

L

L

A

A

V

V

D

D

H

W

F

I

L

V

D

D

M
x
R

G

T

R

T

S

T

A

V

T

V

N

N

V

V

V

E

G

G

N

D

A

A

V

L

A

G

S

A

V

G

I

I

V

L

A

H

-

H

-

L

K

N

W

Q

E

K

G

A

G

T

R

K

L

K

D

G

E

E

I

Q

E

E

P

L

A

A

E

E

I

V

E

K

P

V

R

E

H

A

A

I

P

P

N201 (= N150) modified: carbohydrate, N-linked (GlcNAc...) asparagine
N206 (vs. gap) modified: carbohydrate, N-linked (GlcNAc...) asparagine
E256 (≠ Q185) to K: in SPATCCM; does not affect localization at the cell surface; decreased uptake of L-serine and L-alanine; Vmax is decreased by at least 50% for both substrates; 3-fold increase of affinity for L-serine; 2-fold increase of affinity for L-alanine; dbSNP:rs201278558
R457 (≠ M382) to W: in SPATCCM; does not affect localization at the cell surface; loss of uptake of L-serine and L-alanine; dbSNP:rs761533681

7xr6A Structure of human excitatory amino acid transporter 2 (eaat2) in complex with way-213613 (see paper)
28% identity, 90% coverage: 35:411/421 of query aligns to 38:422/424 of 7xr6A

query
sites
7xr6A

D

D

V

V

T

V

G

M

H

L

I

I

S

A

I

F

L

P

T

G

E

D

L

I

F

L

L

M

R

R

L

M

I

L

K

K

M

M

I

L

I

I

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

L

V

I

T

T

G

G

I

L

A

S

H

G

M

L

G

-

D

D

T

A

A

K

A

A

L

S

G

G

R
|
R

V

L

G

G

F

T

R
|
R

S

A

I

M

A

V

W

Y

F

Y

L

M

T

S

A

T

S

T

L

I

M
x
I

S

A

L

A

T

V

L

L

G

G

L

V

I

I

M

L

V

V

N

L

L

A

L

I

Q

H

P

P

G

G

V

N

G

P

A

K

-

V

-

S

-

L

-

D

-

A

-

F

-

L

D

D

L

L

V

I

-

R

-

N

L

L

P

F

P

P

E

E

G

N

A

-

S

-

A

-

G

-

V

L

S

V

Q

Q

A

A

D

C

F

F

S

Q

L

Q

K

I

Q

Q

F

T

V

V

T

T

H

K

L

V

V

I

P

K

K

K

-

G

-

L

S

E

F

F

F

K

E

D

A

G

M

M

A

-

T

-

N

-

E

N

I

V

L

L

Q

G

I

L

V

I

V

G

F

F

S

F

V

I

F

A

T

F

G

G

V

I

A

A

I

M

T

G

A

K

V

M

G

G

E

D

R

Q

A

A

A

K

P

L

L

M

V

V

R

D

G

F

I

F

E

N

A

I

L

L

V

N

Q

E

V

I

M

V

L

M

Q

K

I

L

T

V

D

I

Y

M

V

I

M

M

R

W

F

Y

A

S

P

P

F

L

A

G

V

I

F

A

A

C

A

L

V

I

T

C

T

G

A

K

L

I

A

I

-

A

-

I

E

K

Q

D

G

L

P

E

G

V

I

V

L

A

L

R

S

Q

F

L

G

G

K

M

F

Y

M

M

G

V

S

T

F

V

Y

I

L

I

S

G

M

L

F

I

L

I

L

H

W

G

G

G

L

I

L

F

L

L

L

P

L

L

C
x
I

Y

Y

L

F

I

V

V

V

G

T

G

R

R

K

S

N

K

P

L

F

-

S

L

F

I

F

R

A

Y

G

I

I

R

F

E

Q

P

A

I

W

L

I

L

T

A

A

F

L

S

G

T

T

A

A

S
|
S

E

A

A

G

A

T

F

L

P

P

R

V

T

T

L

F

E

R

A

C

L

L

D

E

-

E

R

N

F

L

G

G

V

I

P

D

P

K

R

R

I

V

A

T

S

R

F

F

V

V

L

L

P

P

L

V

G

G

Y

A

S

T

F

I

N

N

L

M

D

D

G

G

S
x
T

M

A

I

L

Y

Y

C

E

T

A

F

V

A

A

T

A

M

I

F

F

I

I

A

A

Q

Q

A

M

Y

N

G

G

I

V

E

V

L

L

T

D

F

G

A

G

H

Q

Q

I

V

V

T

T

M

V

L

S

L

L

I

T

L

A

M

T

V

L

T

A

S

S

K

V

G

G

I

A

A

A

G

S

V

I

P

P

R

S

A

A

S
x
G

L

L

V

V

I

T

I
x
M

S

L

T

I

L

L

A

T

F

A

F

V

D

G

I

L

P

P

E

T

A

E

G

D

L

I

L

S

L

L

I

L

L
x
V

A

A

V

V

D
|
D

H
x
W

F

L

L

L

D

D

M

R

G

M

R
|
R

S
x
T

A

S

T

V

N

N

V

V

G

G

N

D

A

S

V

F

A

G

S

A

V

G

I

I

V

V

A

Y

K

H

W

L

E

S

G

K

G

S

R

E

L

L

D

D

E

T

I

I

E

D

binding (2S)-2-azanyl-4-[[4-[2-bromanyl-4,5-bis(fluoranyl)phenoxy]phenyl]amino]-4-oxidanylidene-butanoic acid: S280 (= S270), S281 (= S271), T318 (≠ S307), G363 (≠ S352), M367 (≠ I356), V385 (≠ L374), D388 (= D377), R395 (= R384), T396 (≠ S385)
binding dodecyl beta-D-glucopyranoside: W389 (≠ H378)
binding cholesterol hemisuccinate: R80 (= R78), R84 (= R82), I95 (≠ M93), I252 (≠ C243)

Sites not aligning to the query:

binding dodecyl beta-D-glucopyranoside: 16, 19, 20

8cv2A Human excitatory amino acid transporter 3 (eaat3) in an outward facing sodium-bound state (see paper)
27% identity, 96% coverage: 8:411/421 of query aligns to 6:431/433 of 8cv2A

query
sites
8cv2A

Y

W

I

V

L

L

G

L

A

S

M

T

I

V

A

A

G

A

I

V

V

V

V

L

G

G

I

I

T

T

L

T

N

G

R

V

T

L

I

V

T

R

D

E

P

H

A

S

T

N

L

L

T

S

D

T

V

L

T

E

G

K

-

F

H

Y

I

F

S

A

I

F

L

P

T

G

E

E

L

I

F

L

L

M

R

R

L

M

I

L

K

K

M

L

I

I

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

M

V

I

T

T

G

G

I

V

A

A

H

A

M

L

G

-

D

D

T

S

A

N

A

V

L

S

G

G

R

K

V

I

G

G

F

L

R

R

S

A

I

V

A

V

W

Y

F
x
Y

L

F

T

A

A
x
T

S
x
T

L

L

M

I

S

A

L

V

T

I

L

L

G

G

L

I

I

V

M

L

V

V

N

V

L

S

L

I

Q

K

P

P

G

G

V

V

G

T

A

Q

D

K

L

V

-

G

V

E

L

I

P

A

P

R

E

T

G

G

A

S

S

T

A

P

G

E

V

V

S

S

Q

T

A

V

D

D

F

A

S

M

L

L

-

D

-

L

-

I

-

R

K

N

Q

M

F

F

V

P

T

E

H

N

L

L

V

V

P

Q

K

A

S

A

F

F

F

Q

E

Q

A

Y

M

K

A

T

T

K

N

R

E

E

-

E

-

Y

-

K

-

I

-

V

-

G

-

M

-

Y

-

S

-

D

-

G

-

I

-

N

I

V

L

L

Q

G

I

L

V

I

V

V

F

F

S

A

V

L

F

V

T

F

G

G

V

L

A

V

I

I

T

G

A

K

V

M

G

G

E

E

R

K

A

G

A

Q

P

I

L

L

V

V

R

D

G

F

I

F

E

N

A

A

L

L

V

S

Q

D

V

A

M

T

L

M

Q

K

I

I

T

V

D

Q

Y

I

V

I

M

M

R

W

F

Y

A

M

P

P

F

L

A

G

V

I

F

L

A

F

A

L

V

I

T

A

T

G

A

C

L

I

A

I

E

E

-

V

Q

E

G

D

P

W

G

E

I

I

L

F

L

R

S

K

F

L

G

G

K

L

F

Y

M

M

G

A

S

T

F

V

Y

L

L

T

S

G

M

L

F

A

L

I

L

H

W

S

G

I

L

V

L

I

L

L

L

P

L

L

C

I

Y

Y

L

F

I

I

V

V

G

V

G

R

R

K

S

N

K

P

L

F

L

-

I

-

R

R

Y

F

-

A

-

M

-

G

I

M

R

A

E

Q

P

A

I

L

L

L

L

T

A

A

F

L

S

M

T

I

A

S

S

S

E

S

A

A

A

T

F

L

P

P

R

V

T

T

L

F

E

R

-

C

A

A

L

E

D

E

R

N

F

N

G

Q

V

V

P

D

P

K

R

R

I

I

A

T

S

R

F

F

V

V

L

L

P

P

L

V

G
|
G

Y
x
A

S

T

F

I

N
|
N

L
x
M

D
|
D

G

G

S

T

M

A

I

L

Y

Y

C

E

T

A

F

V

A

A

T

A

M

V

F

F

I

I

A

A

Q

Q

A

L

Y

N

G

D

I

L

E

D

L

L

T

G

F

I

A

G

H

Q

Q

I

V

I

T

T

M

I

L

S

L

I

I

T

L

A

M

T

V

S

T

A

S

S

K

I

G

G

I

A

A

A

G

G

V

V

P

P

R

Q

A

A

S

G

L

L

V

V

I

T

I

M

S

V

S

I

T

V

L

L

A

S

F

A

F

V

D

G

I

L

P

P

E

A

A

E

G

D

L

V

L

T

L

L

I

I

L

I

A

A

V

V

D

D

H

C

F

L

L

L

D

D

M

R

G

F

R

R

S

T

A

M

T

V

N
|
N

V

V

V

L

G

G

N
x
D

A

A

V

F

A

G

S

T

V

G

I

I

V

V

A

E

K

K

W

L

E

S

G

K

R

E

L

L

D

E

E

Q

I

M

E

D

binding sodium ion: Y85 (≠ F87), T88 (≠ A90), T89 (≠ S91), G319 (= G299), A320 (≠ Y300), N323 (= N303), N323 (= N303), M324 (≠ L304), D325 (= D305), N408 (= N388), D412 (≠ N392)

9d68A Human excitatory amino acid transporter 3 (eaat3) with bound l- cysteine in an outward facing state (see paper)
27% identity, 96% coverage: 8:410/421 of query aligns to 1:425/425 of 9d68A

query
sites
9d68A

Y

W

I

V

L

L

G

L

A

S

M

T

I

V

A

A

G

A

I

V

V

V

V

L

G

G

I

I

T

T

L

T

N

G

R

V

T

L

I

V

T

R

D

E

P

H

A

S

T

N

L

L

T

S

D

T

V

L

T

E

G

K

-

F

H

Y

I

F

S

A

I

F

L

P

T

G

E

E

L

I

F

L

L

M

R

R

L

M

I

L

K

K

M

L

I

I

A

L

P

P

L

L

V

I

F

I

A

S

T

S

L

M

V

I

T

T

G

G

I

V

A

A

H

A

M

L

G

-

D

D

T

S

A

N

A

V

L

S

G

G

R

K

V

I

G

G

F

L

R

R

S

A

I

V

A

V

W

Y

F
x
Y

L

F

T

A

A
x
T

S
x
T

L

L

M

I

S

A

L

V

T

I

L

L

G

G

L

I

I

V

M

L

V

V

N

V

L

S

L

I

Q

K

P

P

G

G

V

V

G

T

A

Q

D

K

L

V

-

G

V

E

L

I

P

A

P

R

E

T

G

G

A

S

S

T

A

P

G

E

V

V

S

S

Q

T

A

V

D

D

F

A

S

M

L

L

-

D

-

L

-

I

-

R

K

N

Q

M

F

F

V

P

T

E

H

N

L

L

V

V

P

Q

K

A

S

A

F

F

F

Q

E

Q

A

Y

M

K

A

T

T

K

N

R

E

E

-

E

-

Y

-

K

-

I

-

V

-

G

-

M

-

Y

-

S

-

D

-

G

-

I

-

N

I

V

L

L

Q

G

I

L

V

I

V

V

F

F

S

A

V

L

F

V

T

F

G

G

V

L

A

V

I

I

T

G

A

K

V

M

G

G

E

E

R

K

A

G

A

Q

P

I

L

L

V

V

R

D

G

F

I

F

E

N

A

A

L

L

V

S

Q

D

V

A

M

T

L

M

Q

K

I

I

T

V

D

Q

Y

I

V

I

M

M

R

W

F

Y

A

M

P

P

F

L

A

G

V

I

F

L

A

F

A

L

V

I

T

A

T

G

A

C

L

I

A

I

E

E

-

V

Q

E

G

D

P

W

G

E

I

I

L

F

L

R

S

K

F

L

G

G

K

L

F

Y

M

M

G

A

S

T

F

V

Y

L

L

T

S

G

M

L

F

A

L

I

L

H

W

S

G

I

L

V

L

I

L

L

L

P

L

L

C

I

Y

Y

L

F

I

I

V

V

G

V

G

R

R

K

S

N

K

P

L

F

L

-

I

-

R

R

Y

F

-

A

-

M

-

G

I

M

R

A

E

Q

P

A

I

L

L

L

L

T

A

A

F

L

S

M

T

I

A

S

S
|
S

E

S

A

A

A

T

F

L

P

P

R

V

T

T

L

F

E

R

-

C

A

A

L

E

D

E

R

N

F

N

G

Q

V

V

P

D

P

K

R

R

I

I

A

T

S

R

F

F

V

V

L

L

P

P

L

V

G
|
G

Y
x
A

S

T

F

I

N
|
N

L

M

D
|
D

G

G

S
x
T

M

A

I

L

Y

Y

C

E

T

A

F

V

A

A

T

A

M

V

F

F

I

I

A

A

Q

Q

A

L

Y

N

G

D

I

L

E

D

L

L

T

G

F

I

A

G

H

Q

Q

I

V

I

T

T

M

I

L

S

L

I

I

T

L

A

M

T

V

S

T

A

S

S

K

I

G

G

I

A

A
|
A

G

G

V

V

P

P

R

Q

A

A

S

G

L

L

V

V

I

T

I

M

S

V

S

I

T

V

L

L

A

S

F

A

F

V

D

G

I

L

P

P

E

A

A

E

G

D

L

V

L

T

L

L

I

I

L

I

A

A

V

V

D

D

H

C

F

L

L

L

D
|
D

M

R

G

F

R
|
R

S
x
T

A

M

T

V

N
|
N

V

V

V

L

G

G

N
x
D

A

A

V

F

A

G

S

T

V

G

I

I

V

V

A

E

K

K

W

L

E

S

G

K

R

E

L

L

D

E

E

Q

I

M

binding cysteine: S284 (= S270), S285 (= S271), T322 (≠ S307), A361 (= A346), D396 (= D381), R399 (= R384), T400 (≠ S385), N403 (= N388)
binding sodium ion: Y80 (≠ F87), T83 (≠ A90), T84 (≠ S91), G314 (= G299), A315 (≠ Y300), N318 (= N303), N318 (= N303), D320 (= D305), N403 (= N388), D407 (≠ N392)

Query Sequence

>WP_011952050.1 NCBI__GCF_000016765.1:WP_011952050.1
MAKRLTYYILGAMIAGIVVGITLNRTITDPATLTDVTGHISILTELFLRLIKMIIAPLVF
ATLVTGIAHMGDTAALGRVGFRSIAWFLTASLMSLTLGLIMVNLLQPGVGADLVLPPEGA
SAGVSQADFSLKQFVTHLVPKSFFEAMATNEILQIVVFSVFTGVAITAVGERAAPLVRGI
EALVQVMLQITDYVMRFAPFAVFAAVTTALAEQGPGILLSFGKFMGSFYLSMFLLWGLLL
LLCYLIVGGRSKLLIRYIREPILLAFSTASSEAAFPRTLEALDRFGVPPRIASFVLPLGY
SFNLDGSMIYCTFATMFIAQAYGIELTFAHQVTMLLILMVTSKGIAGVPRASLVIISSTL
AFFDIPEAGLLLILAVDHFLDMGRSATNVVGNAVASVIVAKWEGGRLDEIEPAEIEPRHA
P

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory