SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011970089.1 NCBI__GCF_000017145.1:WP_011970089.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P08308 Ornithine carbamoyltransferase, catabolic; OTCase; EC 2.1.3.3 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 4 papers)
69% identity, 100% coverage: 1:334/334 of query aligns to 1:335/336 of P08308

query
sites
P08308

M
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M

S

A

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F

N

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R

H

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C

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A

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F

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E

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D

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A

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D

D

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D

M1 (= M1) modified: Initiator methionine, Removed
E106 (= E106) mutation E->A,G: Loss of homotropic cooperativity; gain of anabolic activity. Conformational change which modifies the catalytic site. This mutant is blocked in the active R (relaxed) state.

4jfrB Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with carbamoyl phosphate
59% identity, 100% coverage: 1:333/334 of query aligns to 7:339/340 of 4jfrB

query
sites
4jfrB

M

M

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F

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N

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R

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N

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R

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N

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D

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M

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E

D

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Q

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C

D

D

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D
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D

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V

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W

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M

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T

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G

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M

H

H

C
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C

L

L

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Y

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T

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E

E

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T

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K

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D

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N

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T

T

D

E

E

E

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V

F

F

E

E

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H

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S

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R
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R

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M

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H

T

T

I

I

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K

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A

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L

G

G

active site: R65 (= R59), T66 (= T60), D93 (≠ H87), R114 (= R108), H141 (= H135), Q144 (= Q138), D239 (= D232), C281 (= C274), R326 (= R320)
binding phosphoric acid mono(formamide)ester: S63 (= S57), T64 (= T58), R65 (= R59), T66 (= T60), R114 (= R108), H141 (= H135), Q144 (= Q138), C281 (= C274), R326 (= R320)

4jhxA Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with carbamoylphosphate and arginine
59% identity, 100% coverage: 1:333/334 of query aligns to 3:335/336 of 4jhxA

query
sites
4jhxA

M

M

S

A

F

F

N

N

L

L

R

R

N

N

R

R

S

N

L

F

L

L

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K

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L

Q

L

D

D

Y

F

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P

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R

K

E

E

F

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R

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Y

F

L

L

V

I

D

D

L

L

A

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R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

A

A

R

G

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T

E

E

Q

Q

E

K

H

K

L

L

K

L

G

G

K

K

E

N

I

I

C

A

L

L

I

I

F

F

E

E

K

K

T

A

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T
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T

R
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T
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T

R

R

C

C

A

A

F

F

E

E

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A

C

A

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F

D

D

Q

Q

G

G

A

A

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Y

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I

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P

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G

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H
x
D

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K

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E

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M

K

K

D

D

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T

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A

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R

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L

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G

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R

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M

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D

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L

A

G

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A

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F

A

A

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G

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W

N

N

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L
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L

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T

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D

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T

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Y

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A

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N
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G

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E

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A

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A

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I

K

A

A

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T

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G

A

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M

T

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L

T

T

D

E

D

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P

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A

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C

D

D

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F

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D

V

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W

W

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S
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S

M
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M

G

G

E

E

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P

E

E

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A

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W

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D

E

E

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A

L

L

L

M

T

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P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

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M

L

A

K

A

Q

S

T

G

G

N

N

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P

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N

T

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K

F

F

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M

H

H

C
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C

L
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L

P
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P

A

A

Y

F

H

H

D

N

T

D

E

E

T

T

A

T

I

I

G

G

K

K

Q

Q

I

V

S

A

E

D

D

K

Y

F

G

G

M

M

S

K

N

-

G

G

L

L

E

E

V

V

T

T

D

E

E

E

V

V

F

F

E

E

S

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Q

E

A

H

N

S

V

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A

V

F

F

E

D

Q

E

A

A

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E

N

N

R
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R

M

M

H

H

T

T

I

I

K

K

A

A

L

V

L

M

V

V

A

A

T

T

L

L

G

G

active site: R61 (= R59), T62 (= T60), D89 (≠ H87), R110 (= R108), H137 (= H135), Q140 (= Q138), D235 (= D232), C277 (= C274), R322 (= R320)
binding arginine: L132 (= L130), N171 (= N168), D235 (= D232), S239 (= S236), M240 (= M237), P279 (= P276)
binding phosphoric acid mono(formamide)ester: S59 (= S57), T60 (= T58), R61 (= R59), T62 (= T60), R110 (= R108), H137 (= H135), C277 (= C274), L278 (= L275), R322 (= R320)

4jqoA Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with citrulline and inorganic phosphate
59% identity, 100% coverage: 1:333/334 of query aligns to 5:337/338 of 4jqoA

query
sites
4jqoA

M

M

S

A

F

F

N

N

L

L

R

R

N

N

R

R

S

N

L

F

L

L

T

K

V

L

Q

L

D

D

Y

F

T

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E

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L

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I

D

D

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L

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A

D

D

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A

A

K

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Y

Y

A

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E

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H

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L

L

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L

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G

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K

E

N

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A

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L

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I

F

F

E

E

K

K

T

A

S

S

T

T

R
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R

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T

R

R

C

C

A

A

F

F

E

E

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V

A

A

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A

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F

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D

Q

Q

G

G

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A

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Y

L

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Q

Q

I

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H
x
D

K

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E

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K

D

D

T

T

A

A

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V

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L

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G

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R

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M

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Y

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D

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A

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K

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Y

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A

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G

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L

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T

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F

H
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H

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M

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Y

F

A

A

Y

Y

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D

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G

H

N

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L

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V

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G

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C

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Y

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T

A

I

A

A

C

K

Q

E

A

L

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A

A

K

Q

Q

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T

G

G

A

G

R

K

L

I

M

T

I

L

T

T

D

E

D

N

P

V

K

A

A

E

A

G

V

V

E

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

S

K

P

E

E

V

A

W

W

K

D

E

E

R

R

I

V

Q

A

L

L

L

M

T

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

A

K

A

Q

S

T

G

G

N

N

P

P

Q

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

Y

F

H

H

D

N

T

D

E

E

T

T

A

T

I

I

G

G

K

K

Q

Q

I

V

S

A

E

D

D

K

Y

F

G

G

M

M

S

K

N

-

G

G

L

L

E

E

V

V

T

T

D

E

E

E

V

V

F

F

E

E

S

S

Q

E

A

H

N

S

V

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

L

V

L

M

V

V

A

A

T

T

L

L

G

G

active site: R63 (= R59), T64 (= T60), D91 (≠ H87), R112 (= R108), H139 (= H135), Q142 (= Q138), D237 (= D232), C279 (= C274), R324 (= R320)
binding citrulline: H139 (= H135), Q142 (= Q138), N173 (= N168), D237 (= D232), S241 (= S236), M242 (= M237), C279 (= C274), L280 (= L275), R324 (= R320)

Q8DCF5 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Vibrio vulnificus (strain CMCP6)
59% identity, 100% coverage: 1:333/334 of query aligns to 1:333/334 of Q8DCF5

query
sites
Q8DCF5

M

M

S

A

F

F

N

N

L

L

R

R

N

N

R

R

S

N

L

F

L

L

T

K

V

L

Q

L

D

D

Y

F

T

S

P

T

R

K

E

E

F

I

R

Q

Y

F

L

L

V

I

D

D

L

L

A

S

R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

A

A

R

G

T

T

E

E

Q

Q

E

K

H

K

L

L

K

L

G

G

K

K

E

N

I

I

C

A

L

L

I

I

F

F

E

E

K

K

T

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

C

A

S

F

D

D

Q

Q

G

G

A

A

N

Q

V

V

T

T

Y

Y

L

I

D

G

P

P

A

S

G

G

S

S

Q
|
Q

I

I

G

G

H

D

K

K

E

E

S

S

F

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

A

F

S

G

Q

Q

A

A

G

I

V

V

E

E

T

E

L

L

A

G

K

A

Y

F

A

A

G

G

V

V

P

P

V

V

Y

W

N

N

G

G

L

L

T

T

D

D

E

E

Y

F

H
|
H

P
|
P

T
|
T

Q
|
Q

M

I

I

L

A

A

D

D

V

F

M

L

T

T

M

M

R

L

E

E

H

H

S

S

D

Q

-

G

K

K

P

A

I

L

S

A

E

D

I

I

K

Q

Y

F

A

A

Y

Y

I

L

G

G

D

D

T

A

R

R

S

N

N
|
N

M

V

G

G

H

N

S

S

L

L

L

M

I

V

V

G

G

A

C

A

L

K

M

M

G

G

M

M

D

D

V

I

R

R

I

L

C

V

G

G

P

P

R

Q

S

A

L

Y

W

W

P

P

S

D

E

E

Y

L

Q

V

T

A

I

A

A

C

K

Q

E

A

L

I

K

A

A

K

Q

Q

S

T

G

G

A

G

R

K

L

I

M

T

I

L

T

T

D

E

D

N

P

V

K

A

A

E

A

G

V

V

E

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

S

K

P

E

E

V

A

W

W

K

D

E

E

R

R

I

V

Q

A

L

L

L

M

T

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

A

K

A

Q

S

T

G

G

N

N

P

P

Q

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

Y

F

H

H

D

N

T

D

E

E

T

T

A

T

I

I

G

G

K

K

Q

Q

I

V

S

A

E

D

D

K

Y

F

G

G

M

M

S

K

N

-

G

G

L

L

E

E

V

V

T

T

D

E

E

E

V

V

F

F

E

E

S

S

Q

E

A

H

N

S

V

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

L

V

L

M

V

V

A

A

T

T

L

L

G

G

STRT 57:60 (= STRT 57:60) binding carbamoyl phosphate
Q84 (= Q84) binding carbamoyl phosphate
R108 (= R108) binding carbamoyl phosphate
HPTQ 135:138 (= HPTQ 135:138) binding carbamoyl phosphate
N169 (= N168) binding L-ornithine
D233 (= D232) binding L-ornithine
SM 237:238 (= SM 236:237) binding L-ornithine
CL 275:276 (= CL 274:275) binding carbamoyl phosphate
R320 (= R320) binding carbamoyl phosphate

4h31A Crystal structure of anabolic ornithine carbamoyltransferase from vibrio vulnificus in complex with carbamoyl phosphate and l-norvaline
59% identity, 100% coverage: 1:333/334 of query aligns to 3:335/335 of 4h31A

query
sites
4h31A

M

M

S

A

F

F

N

N

L

L

R

R

N

N

R

R

S

N

L

F

L

L

T

K

V

L

Q

L

D

D

Y

F

T

S

P

T

R

K

E

E

F

I

R

Q

Y

F

L

L

V

I

D

D

L

L

A

S

R

A

D

D

L

L

K

K

R

K

A

A

K

K

Y

Y

A

A

R

G

T

T

E

E

Q

Q

E

K

H

K

L

L

K

L

G

G

K

K

E

N

I

I

C

A

L

L

I

I

F

F

E

E

K

K

T

A

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

A

F

F

E

E

V

V

A

A

C

A

S

F

D

D

Q

Q

G

G

A

A

N

Q

V

V

T

T

Y

Y

L

I

D

G

P

P

A

S

G

G

S

S

Q

Q

I

I

G

G

H
x
D

K

K

E

E

S

S

F

M

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

A

F

S

G

Q

Q

A

A

G

I

V

V

E

E

T

E

L

L

A

G

K

A

Y

F

A

A

G

G

V

V

P

P

V

V

Y

W

N

N

G

G

L
|
L

T

T

D

D

E

E

Y

F

H
|
H

P

P

T

T

Q
|
Q

M

I

I

L

A

A

D

D

V

F

M

L

T

T

M

M

R

L

E

E

H

H

S

S

D

Q

-

G

K

K

P

A

I

L

S

A

E

D

I

I

K

Q

Y

F

A

A

Y

Y

I

L

G

G

D

D

T

A

R

R

S

N

N
|
N

M

V

G

G

H

N

S

S

L

L

L

M

I

V

V

G

G

A

C

A

L

K

M

M

G

G

M

M

D

D

V

I

R

R

I

L

C

V

G

G

P

P

R

Q

S

A

L

Y

W

W

P

P

S

D

E

E

Y

L

Q

V

T

A

I

A

A

C

K

Q

E

A

L

I

K

A

A

K

Q

Q

S

T

G

G

A

G

R

K

L

I

M

T

I

L

T

T

D

E

D

N

P

V

K

A

A

E

A

G

V

V

E

Q

G

G

V

C

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

S

K

P

E

E

V

A

W

W

K

D

E

E

R

R

I

V

Q

A

L

L

L

M

T

K

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

N

L

V

M

L

A

K

A

Q

S

T

G

G

N

N

P

P

Q

N

T

V

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

Y

F

H

H

D

N

T

D

E

E

T

T

A

T

I

I

G

G

K

K

Q

Q

I

V

S

A

E

D

D

K

Y

F

G

G

M

M

S

K

N

-

G

G

L

L

E

E

V

V

T

T

D

E

E

E

V

V

F

F

E

E

S

S

Q

E

A

H

N

S

V

I

A

V

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

L

V

L

M

V

V

A

A

T

T

L

L

G

G

active site: R61 (= R59), T62 (= T60), D89 (≠ H87), R110 (= R108), H137 (= H135), Q140 (= Q138), D235 (= D232), C277 (= C274), R322 (= R320)
binding phosphoric acid mono(formamide)ester: S59 (= S57), T60 (= T58), R61 (= R59), T62 (= T60), R110 (= R108), H137 (= H135), Q140 (= Q138), C277 (= C274), L278 (= L275), R322 (= R320)
binding norvaline: L132 (= L130), N171 (= N168), D235 (= D232), S239 (= S236), M240 (= M237)

P04391 Ornithine carbamoyltransferase subunit I; OTCase-1; EC 2.1.3.3 from Escherichia coli (strain K12) (see 7 papers)
57% identity, 98% coverage: 7:332/334 of query aligns to 6:332/334 of P04391

query
sites
P04391

N

H

R

K

S

H

L

F

L

L

T

K

V

L

Q

L

D

D

Y

F

T

T

P

P

R

A

E

E

F

L

R

N

Y

S

L

L

V

L

D

Q

L

L

A

A

R

A

D

K

L

L

K

K

R

A

A

D

K

K

Y

K

A

S

R

G

T

K

E

E

Q

E

E

A

H

K

L

L

K

T

G

G

K

K

E

N

I

I

C

A

L

L

I

I

F

F

E

E

K

K

T

D

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

S

F

F

E

E

V

V

A

A

C

A

S

Y

D

D

Q

Q

G

G

A

A

N

R

V

V

T

T

Y

Y

L

L

D

G

P

P

A

S

G

G

S

S

Q
|
Q

I

I

G

G

H

H

K
|
K

E

E

S

S

F

I

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

A

Y

S

G

Q

Q

A

E

G

I

V

V

E

E

T

T

L

L

A

A

K

E

Y

Y

A

A

G

S

V

V

P

P

V

V

Y

W

N

N

G

G

L

L

T

T

D

N

E

E

Y

F

H
|
H

P
|
P

T
|
T

Q
|
Q

M

L

I

L

A

A

D

D

V

L

M

L

T

T

M

M

R

Q

E

E

H

H

-

L

S

P

D

G

K

K

P

A

I

F

S

N

E

E

I

M

K

T

Y

L

A

V

Y

Y

I

A

G

G

D

D

T

A

R

R

S

N

N
|
N

M

M

G

G

H

N

S

S

L

M

L

L

I

E

V

A

G

A

C

A

L

L

M

T

G

G

M

L

D

D

V

L

R

R

I

L

C

V

G

A

P

P

R

Q

S

A

L

C

W

W

P

P

S

E

E

A

Y

L

Q

V

T

T

I

E

A

C

K

R

E

A

L

L

K

A

A

Q

Q

Q

S

N

G

G

A

G

R

N

L

I

M

T

I

L

T

T

D

E

D

D

P

V

K

A

A

K

A

G

V

V

E

E

G

G

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

A

K

K

E

E

V

K

W

W

K

A

E

E

R

R

I

I

Q

A

L

L

T

R

P

E

Y

Y

Q

Q

V

V

N

N

A

S

E

K

L

M

M

M

A

Q

A

L

S

T

G

G

N

N

P

P

Q

E

T

V

K

K

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

Y

F

H

H

D

D

T

D

E

Q

T

T

A

T

I

L

G

G

K

K

Q

K

I

M

S

A

E

E

D

E

Y

F

G

G

M

L

S

H

N

G

G

G

L

M

E

E

V

V

T

T

D

D

E

E

V

V

F

F

E

E

S

S

Q

A

A

A

N

S

V

I

A

V

F

F

E

D

Q

Q

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A
|
A

L

V

L

M

V

V

A

A

T

T

L

L

S56 (= S57) mutation to H: Much less active than the wild-type.
STRT 56:59 (= STRT 57:60) binding carbamoyl phosphate
R58 (= R59) mutation to G: The mutant is drastically inefficient in catalysis, but affects only moderately the binding of carbamoyl phosphate.
Q83 (= Q84) binding carbamoyl phosphate
K87 (= K88) mutation to Q: Much less active than the wild-type.
R107 (= R108) binding carbamoyl phosphate
HPTQ 134:137 (= HPTQ 135:138) binding carbamoyl phosphate
N168 (= N168) binding L-ornithine
D232 (= D232) binding L-ornithine
SM 236:237 (= SM 236:237) binding L-ornithine
C274 (= C274) binding Zn(2+); mutation to A: Zinc ion is no longer a tight-binding inhibitor and does not promote isomerization.
CL 274:275 (= CL 274:275) binding carbamoyl phosphate
R320 (= R320) binding carbamoyl phosphate; mutation to A: Much less active than the wild-type.
A326 (= A326) mutation to G: Activity greater than the wild-type and Km for ornithwinas increases about twofold.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1duvG Crystal structure of e. Coli ornithine transcarbamoylase complexed with ndelta-l-ornithine-diaminophosphinyl-n-sulphonic acid (psorn) (see paper)
57% identity, 98% coverage: 7:332/334 of query aligns to 5:331/333 of 1duvG

query
sites
1duvG

N

H

R

K

S

H

L

F

L

L

T

K

V

L

Q

L

D

D

Y

F

T

T

P

P

R

A

E

E

F

L

R

N

Y

S

L

L

V

L

D

Q

L

L

A

A

R

A

D

K

L

L

K

K

R

A

A

D

K

K

Y

K

A

S

R

G

T

K

E

E

Q

E

E

A

H

K

L

L

K

T

G

G

K

K

E

N

I

I

C

A

L

L

I

I

F

F

E

E

K

K

T

D

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

S

F

F

E

E

V

V

A

A

C

A

S

Y

D

D

Q

Q

G

G

A

A

N

R

V

V

T

T

Y

Y

L

L

D

G

P

P

A

S

G

G

S

S

Q

Q

I

I

G

G

H

H

K

K

E

E

S

S

F

I

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

A

Y

S

G

Q

Q

A

E

G

I

V

V

E

E

T

T

L

L

A

A

K

E

Y

Y

A

A

G

S

V

V

P

P

V

V

Y

W

N

N

G

G

L
|
L

T

T

D

N

E

E

Y

F

H
|
H

P

P

T

T

Q

Q

M

L

I

L

A

A

D

D

V

L

M

L

T

T

M

M

R

Q

E

E

H

H

-

L

S

P

D

G

K

K

P

A

I

F

S

N

E

E

I

M

K

T

Y

L

A

V

Y

Y

I

A

G

G

D

D

T

A

R

R

S

N

N
|
N

M

M

G

G

H

N

S

S

L

M

L

L

I

E

V

A

G

A

C

A

L

L

M

T

G

G

M

L

D

D

V

L

R

R

I

L

C

V

G

A

P

P

R

Q

S

A

L

C

W

W

P

P

S

E

E

A

Y

L

Q

V

T

T

I

E

A

C

K

R

E

A

L

L

K

A

A

Q

Q

Q

S

N

G

G

A

G

R

N

L

I

M

T

I

L

T

T

D

E

D

D

P

V

K

A

A

K

A

G

V

V

E

E

G

G

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

A

K

K

E

E

V

K

W

W

K

A

E

E

R

R

I

I

Q

A

L

L

T

R

P

E

Y

Y

Q

Q

V

V

N

N

A

S

E

K

L

M

M

M

A

Q

A

L

S

T

G

G

N

N

P

P

Q

E

T

V

K

K

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

Y

F

H

H

D

D

T

D

E

Q

T

T

A

T

I

L

G

G

K

K

Q

K

I

M

S

A

E

E

D

E

Y

F

G

G

M

L

S

H

N

G

G

G

L

M

E

E

V

V

T

T

D

D

E

E

V

V

F

F

E

E

S

S

Q

A

A

A

N

S

V

I

A

V

F

F

E

D

Q

Q

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

L

V

L

M

V

V

A

A

T

T

L

L

binding ndelta-(n'-sulphodiaminophosphinyl)-l-ornithine: S55 (= S57), T56 (= T58), R57 (= R59), T58 (= T60), R106 (= R108), L128 (= L130), H133 (= H135), N167 (= N168), D231 (= D232), S235 (= S236), M236 (= M237), C273 (= C274), L274 (= L275), R319 (= R320)

2otcA Ornithine transcarbamoylase complexed with n-(phosphonacetyl)-l- ornithine (see paper)
57% identity, 98% coverage: 7:332/334 of query aligns to 5:331/333 of 2otcA

query
sites
2otcA

N

H

R

K

S

H

L

F

L

L

T

K

V

L

Q

L

D

D

Y

F

T

T

P

P

R

A

E

E

F

L

R

N

Y

S

L

L

V

L

D

Q

L

L

A

A

R

A

D

K

L

L

K

K

R

A

A

D

K

K

Y

K

A

S

R

G

T

K

E

E

Q

E

E

A

H

K

L

L

K

T

G

G

K

K

E

N

I

I

C

A

L

L

I

I

F

F

E

E

K

K

T

D

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

C

A

S

F

F

E

E

V

V

A

A

C

A

S

Y

D

D

Q

Q

G

G

A

A

N

R

V

V

T

T

Y

Y

L

L

D

G

P

P

A

S

G

G

S

S

Q

Q

I

I

G

G

H
|
H

K

K

E

E

S

S

F

I

K

K

D

D

T

T

A

A

R

R

V

V

L

L

G

G

R

R

M

M

Y

Y

D

D

A

G

I

I

E

Q

Y

Y

R
|
R

G

G

A

Y

S

G

Q

Q

A

E

G

I

V

V

E

E

T

T

L

L

A

A

K

E

Y

Y

A

A

G

R

V

V

P

P

V

V

Y

W

N

N

G

G

L

L

T

T

D

N

E

E

Y

F

H
|
H

P

P

T

T

Q
|
Q

M

L

I

L

A

A

D

D

V

L

M

L

T

T

M

M

R

Q

E

E

H

H

-

L

S

P

D

G

K

K

P

A

I

F

S

N

E

E

I

M

K

T

Y

L

A

V

Y

Y

I

A

G

G

D

D

T

A

R

R

S

N

N
|
N

M

M

G

G

H

N

S

S

L

M

L

L

I

E

V

A

G

A

C

A

L

L

M

T

G

G

M

L

D

D

V

L

R

R

I

L

C

V

G

A

P

P

R

Q

S

A

L

C

W

W

P

P

S

E

E

A

Y

L

Q

V

T

T

I

E

A

C

K

R

E

A

L

L

K

A

A

Q

Q

Q

S

N

G

G

A

G

R

N

L

I

M

T

I

L

T

T

D

E

D

D

P

V

K

A

A

K

A

G

V

V

E

E

G

G

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

V

S
|
S

M
|
M

G

G

E

E

P

A

K

K

E

E

V

K

W

W

K

A

E

E

R

R

I

I

Q

A

L

L

T

R

P

E

Y

Y

Q

Q

V

V

N

N

A

S

E

K

L

M

M

M

A

Q

A

L

S

T

G

G

N

N

P

P

Q

E

T

V

K

K

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

Y

F

H

H

D

D

T

D

E

Q

T

T

A

T

I

L

G

G

K

K

Q

K

I

M

S

A

E

E

D

E

Y

F

G

G

M

L

S

H

N

G

G

G

L

M

E

E

V

V

T

T

D

D

E

E

V

V

F

F

E

E

S

S

Q

A

A

A

N

S

V

I

A

V

F

F

E

D

Q

Q

A

A

E

E

N

N

R
|
R

M

M

H

H

T

T

I

I

K

K

A

A

L

V

L

M

V

V

A

A

T

T

L

L

active site: R57 (= R59), T58 (= T60), H85 (= H87), R106 (= R108), H133 (= H135), Q136 (= Q138), D231 (= D232), C273 (= C274), R319 (= R320)
binding n-(phosphonoacetyl)-l-ornithine: S55 (= S57), T56 (= T58), R57 (= R59), T58 (= T60), R106 (= R108), H133 (= H135), N167 (= N168), D231 (= D232), S235 (= S236), M236 (= M237), L274 (= L275), R319 (= R320)

Q8G998 Ornithine carbamoyltransferase, catabolic; OTCase; EC 2.1.3.3 from Lentilactobacillus hilgardii (Lactobacillus hilgardii) (see paper)
50% identity, 99% coverage: 5:334/334 of query aligns to 10:337/343 of Q8G998

query
sites
Q8G998

L

F

R

Q

N

G

R

R

S

S

L

V

L

L

T

A

V

E

Q

K

D

D

Y

F

T

S

P

A

R

A

E

E

F

L

R

E

Y

Y

L

L

V

I

D

D

L

F

A

G

R

L

D

H

L

L

K

K

R

A

A

L

K

K

Y

K

A

A

R

G

T

I

E

P

Q

H

E

H

H

Y

L

L

K

E

G

G

K

K

E

N

I

I

C

A

L

L

I

L

F

F

E

E

K

K

T

S

S

S

T

T

R

R

T

T

R

R

C

S

A

A

F

F

E

T

V

T

A

A

C

S

S

I

D

D

Q

L

G

G

A

A

N
x
H

V

P

T

E

Y

Y

L

L

D

G

P

Q

A

N

G

D

S

I

Q

Q

I

L

G

G

H

K

K

K

E

E

S

S

F

T

K

S

D

D

T

T

A

A

R

K

V

V

L

L

G

G

R

S

M

M

Y

F

D

D

A

G

I

I

E

E

Y

F

R

R

G

G

A

F

S

K

Q

Q

A

S

G

D

V

A

E

E

T

I

L

L

A

A

K

R

Y

D

A

S

G

G

V

V

P

P

V

V

Y

W

N

N

G

G

L

L

T

T

D

D

E

E

Y

W

H

H

P

P

T

T

Q

Q

M

M

I

L

A

A

D

D

V

F

M

M

T

T

M

V

R

K

E

E

H

N

S

F

D

G

K

K

P

-

I

L

S

Q

E

G

I

L

K

T

Y

L

A

T

Y

F

I

M

G

G

D

D

T

G

R

R

S

N

N

N

M

V

G

A

H

N

S

S

L

L

I

V

V

T

G

G

C

A

L

I

M

L

G

G

M

V

D

N

V

I

R

H

I

I

C

V

G

A

P

P

R

K

S

A

L

L

W

F

P

P

S

T

E

E

Y

T

Q

Q

T

N

I

I

A

A

K

K

E

G

L

F

K

A

A

E

Q

K

S

S

G

G

A

A

R

K

L

L

M

V

I

I

T

T

D

D

P

L

K

D

A

E

A

G

V

L

E

K

G

G

V

S

D

N

F

V

I

V

H

Y

T

T

D

D

V

V

W

W

V

V

S

S

M

M

G

G

E

E

P

S

K

N

E

-

V

-

W

W

K

E

E

E

R

R

I

V

Q

K

L

E

L

L

T

T

P

P

Y

Y

Q

Q

V

V

N

N

A

M

E

E

L

A

M

M

A

K

S

T

G

G

N

T

P

P

-

D

Q

Q

T

L

K

I

F

F

M

M

H

H

C

C

L

L

P

P

A

A

Y

F

H

H

D

N

T

T

E

D

T

T

A

Q

I

Y

G

G

K

K

Q

E

I

I

S

K

E

E

D

K

Y

Y

G

G

M

I

S

T

N

E

G

-

L

M

E

E

V

V

T

T

D

D

E

E

V

V

F

F

E

T

S

S

Q

K

A

Y

N

A

V

R

A

Q

F

F

E

E

Q

E

A

A

E

E

N

N

R

R

M

M

H

H

T

S

I

I

K

K

A

A

L

M

V

A

A

A

T

T

L

L

G

G

D
x
N

H79 (≠ N74) binding Ni(2+)

Sites not aligning to the query:

337:343 mutation Missing: It generates a metastable mutant that behaves as a mixture of monomeric and trimeric species with only the latter exhibiting OTC activity.

Q51742 Ornithine carbamoyltransferase, anabolic; OTCase; EC 2.1.3.3 from Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1) (see 3 papers)
40% identity, 100% coverage: 1:333/334 of query aligns to 1:311/315 of Q51742

query
sites
Q51742

M
|
M

S

V

F

V

N

S

L

L

R

A

N

G

R

R

S

D

L

L

T

C

V

L

Q

Q

D

D

Y

Y

T

T

P

A

R

E

E

E

F

I

R
x
W

Y

T

L

I

V

L

D
x
E

L

T

A

A

R

K

D
x
M

L

F

K

K

R

I

A
x
W

K

Q

Y

K

A

I

R

G

T

K

E

P

Q

H

E

R

H

L

L

L

K

E

G

G

K

K

E

T

I

L

C

A

L

M

I

I

F

F

E

Q

K

K

T

P

S

S

T

T

R

R

T

T

R

R

C

V

A

S

F

F

E

E

V

V

A

A

C

M

S

A

D

H

Q

L

G

G

A

G

N

H

V

A

T

L

Y

Y

L

L

D

N

P

A

A

Q

G

D

S

L

Q

Q

I

L

G

R

H

R

K

G

E

E

S

T

F

I

K

A

D

D

T

T

A

A

R

R

V

V

L

L

G

S

R

R

M

Y

Y

V

D

D

A

A

I

I

E

M

Y

A

R

R

G

V

A

Y

S

D

Q

H

A

K

G

D

V

V

E

E

T

D

L

L

A

A

K

K

Y

Y

A

A

G

T

V

V

P

P

V

V

Y

I

N

N

G

G

L

L

T

S

D

D

E

F

Y

S

H

H

P

P

T

C

Q

Q

M

A

I

L

A

A

D

D

V

Y

M

M

T

T

M

I

R

W

E

E

H

K

S

K

D

G

K

T

P

-

I

I

S

K

E

G

I

V

K

K

Y

V

A

V

Y

Y

I

V

G

G

D

D

T

G

R

-

S

N

N

N

M

V

G

A

H

H

S

S

L

L

L

M

I

I

V

A

G

G

C

T

L

K

M

L

G

G

M

A

D

D

V

V

R

V

I

V

C

A

G

T

P

P

R

E

S

G

L

Y

W

E

P

P

S

D

E

E

E

K

Y

V

Q

I

T

K

I

W

A

A

K

E

E

Q

L

N

K

A

A

A

Q

E

S

S

G

G

A

G

R

S

L

F

M

E

I

L

T

L

D

H

D

D

P

P

K

V

A

K

A

A

V

V

E

K

G

D

V

A

D

D

F

V

I

I

H
x
Y

T

T

D

D

V

V

W

W

V

A

S

S

M

M

G

G

E

Q

P

E

K

A

E

E

V
x
A

W

-

K

E

E

E

R

R

I

R

Q

K

L

I

L

F

T

R

P

P

Y

F

Q

Q

V

V

N

N

A

K

E

D

L

L

M

V

A

-

A

K

S

H

G

A

N

K

P

P

Q

D

T

Y

K

M

F

F

M

M

H

H

C

C

L

L

P

P

A

A

Y

H

H

-

D

-

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

-

N

R

G

G

L

E

E
|
E

V

V

T

T

D

D

E

D

V

V

F

I

E

D

S

S

Q

P

A

N

N

S

V

V

A

V

F

W

E

D

Q

Q

A

A

E

E

N

N

R

R

M

L

H

H

T

A

I

Q

K

K

A

A

L

V

L

L

V

A

A

L

T

V

L

M

G

G

M1 (= M1) modified: Initiator methionine, Removed
W22 (≠ R22) mutation to A: Decreased heat stability.
E26 (≠ D26) mutation to Q: Increased dissociation of dodecamers into trimers.
M30 (≠ D30) mutation to A: Increased dissociation of dodecamers into trimers.
W34 (≠ A34) mutation to A: Increased dissociation of dodecamers into trimers.
Y228 (≠ H230) mutation to C: Becomes active at low temperatures; when associated with G-278.
A241 (≠ V243) mutation to D: Becomes active at low temperatures; when associated with G-278.
E278 (= E300) mutation to G: Becomes active at low temperatures; when associated with C-228 or D-241.

Q81M99 Ornithine carbamoyltransferase; OTCase; EC 2.1.3.3 from Bacillus anthracis
39% identity, 98% coverage: 5:330/334 of query aligns to 9:307/316 of Q81M99

query
sites
Q81M99

L

L

R

N

N

T

R

K

S

D

L

L

T

T

V

L

Q

E

D

E

Y

L

T

T

P

Q

R

E

E

E

F

I

R

I

Y

S

L

L

V

I

D

E

L

F

A

A

R

I

D

Y

L

L

K

K

R

K

A

N

K

K

Y

-

A

-

R

-

T

-

E

Q

Q

E

E

P

H

L

L

L

K

Q

G

G

K

K

E

I

I

L

C

G

L

L

I

I

F

F

E

D

K

K

T

H

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

V

A

S

F

F

E

E

V

A

A

G

C

M

S

V

D

Q

Q

L

G

G

A

G

N

H

V

G

T

M

Y

F

L

L

D

N

P

G

A

K

G

E

S

M

Q
|
Q

I

M

G

G

H

R

K

G

E

E

S

T

F

V

K

S

D

D

T

T

A

A

R

K

V

V

L

L

G

S

R

H

M

Y

Y

I

D

D

A

G

I

I

E

M

Y

I

R
|
R

G

T

A

F

S

S

Q

H

A

A

G

D

V

V

E

E

T

E

L

L

A

A

K

K

Y

E

A

S

G

S

V

I

P

P

V

V

Y

I

N

N

G

G

L

L

T

T

D

D

E

D

Y

H

H
|
H

P
|
P

T
x
C

Q
|
Q

M

A

I

L

A

A

D

D

V

L

M

M

T

T

M

I

R

Y

E

E

H

E

S

T

D

N

K

T

P

-

I

F

S

K

E

G

I

I

K

K

Y

L

A

A

Y

Y

I

V

G

G

D

D

T

G

R

-

S

N

N
|
N

M

V

G

C

H

H

S

S

L

L

I

L

V

A

G

S

C

A

L

K

M

V

G

G

M

M

D

H

V

M

R

T

I

V

C

A

G

T

P

P

R

V

S

G

L

Y

W

K

P

P

S

N

E

E

Y

I

Q

V

T

K

I

K

A

A

K

L

E

A

L

I

K

A

A

K

Q

E

S

T

G

G

A

A

R

E

L

I

M

E

I

I

T

L

D

H

D

N

P

P

K

E

A

L

A

A

V

V

E

N

G

E

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

M

S
|
S

M
|
M

G

G

E

Q

P

E

K

G

E

E

V

-

W

-

K

E

E

E

R

K

I

Y

Q

T

L

L

L

F

T

Q

P

P

Y

Y

Q

Q

V

I

N

N

A

K

E

E

L

L

M

V

A

K

A

H

S

A

G

-

N

-

P

K

Q

Q

T

T

-

Y

K

H

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

Y

H

H

R

D

E

T

E

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

-

N

-

G

-

L

-

E

E

V

V

T

T

D

G

E

E

V

I

F

I

E

D

S

G

Q

P

A

Q

N

S

V

I

A

V

F

F

E

E

Q

Q

A

A

E

G

N

N

R
|
R

M

L

H

H

T

A

I

Q

K

K

A

A

L

L

V

V

A

S

STRT 57:60 (= STRT 57:60) binding carbamoyl phosphate
Q84 (= Q84) binding carbamoyl phosphate
R108 (= R108) binding carbamoyl phosphate
HPCQ 135:138 (≠ HPTQ 135:138) binding carbamoyl phosphate
N166 (= N168) binding L-ornithine
D230 (= D232) binding L-ornithine
SM 234:235 (= SM 236:237) binding L-ornithine
CL 269:270 (= CL 274:275) binding carbamoyl phosphate
R297 (= R320) binding carbamoyl phosphate

4nf2A Crystal structure of anabolic ornithine carbamoyltransferase from bacillus anthracis in complex with carbamoyl phosphate and l- norvaline
39% identity, 98% coverage: 5:330/334 of query aligns to 5:303/307 of 4nf2A

query
sites
4nf2A

L

L

R

N

N

T

R

K

S

D

L

L

T

T

V

L

Q

E

D

E

Y

L

T

T

P

Q

R

E

E

E

F

I

R

I

Y

S

L

L

V

I

D

E

L

F

A

A

R

I

D

Y

L

L

K

K

R

K

A

N

K

K

Y

-

A

-

R

-

T

-

E

Q

Q

E

E

P

H

L

L

L

K

Q

G

G

K

K

E

I

I

L

C

G

L

L

I

I

F

F

E

D

K

K

T

H

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

V

A

S

F

F

E

E

V

A

A

G

C

M

S

V

D

Q

Q

L

G

G

A

G

N

H

V

G

T

M

Y

F

L

L

D

N

P

G

A

K

G

E

S

M

Q

Q

I

M

G

Q

H
x
R

K

G

E

E

S

T

F

V

K

S

D

D

T

T

A

A

R

K

V

V

L

L

G

S

R

H

M

Y

Y

I

D

D

A

G

I

I

E

M

Y

I

R
|
R

G

T

A

F

S

S

Q

H

A

A

G

D

V

V

E

E

T

E

L

L

A

A

K

K

Y

E

A

S

G

S

V

I

P

P

V

V

Y

I

N

N

G

G

L
|
L

T

T

D

D

E

D

Y

H

H
|
H

P

P

T

C

Q
|
Q

M

A

I

L

A

A

D

D

V

L

M

M

T

T

M

I

R

Y

E

E

H

E

S

T

D

N

K

T

P

-

I

F

S

K

E

G

I

I

K

K

Y

L

A

A

Y

Y

I

V

G

G

D

D

T

G

R

-

S

N

N
|
N

M

V

G

C

H

H

S

S

L

L

I

L

V

A

G

S

C

A

L

K

M

V

G

G

M

M

D

H

V

M

R

T

I

V

C

A

G

T

P

P

R

V

S

G

L

Y

W

R

P

P

S

N

E

E

Y

I

Q

V

T

K

I

K

A

A

K

L

E

A

L

I

K

A

A

K

Q

E

S

T

G

G

A

A

R

E

L

I

M

E

I

I

T

L

D

H

D

N

P

P

K

E

A

L

A

A

V

V

E

N

G

E

V

A

D

D

F

F

I

I

H

Y

T

T

D
|
D

V

V

W

W

V

M

S
|
S

M
|
M

G

G

E

Q

P

E

K

G

E

E

V

-

W

-

K

E

E

E

R

K

I

Y

Q

T

L

L

L

F

T

Q

P

P

Y

Y

Q

Q

V

I

N

N

A

K

E

E

L

L

M

V

A

K

A

H

S

A

G

-

N

-

P

K

Q

Q

T

T

-

Y

K

H

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

A

Y

H

H

R

D

E

T

E

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

-

N

-

G

-

L

-

E

E

V

V

T

T

D

G

E

E

V

I

F

I

E

D

S

G

Q

P

A

Q

N

S

V

I

A

V

F

F

E

E

Q

Q

A

A

E

G

N

N

R
|
R

M

L

H

H

T

A

I

Q

K

K

A

A

L

L

V

V

A

S

active site: R55 (= R59), T56 (= T60), R83 (≠ H87), R104 (= R108), H131 (= H135), Q134 (= Q138), D226 (= D232), C265 (= C274), R293 (= R320)
binding phosphoric acid mono(formamide)ester: S53 (= S57), T54 (= T58), R55 (= R59), T56 (= T60), R104 (= R108), H131 (= H135), Q134 (= Q138), C265 (= C274), L266 (= L275), R293 (= R320)
binding norvaline: L126 (= L130), N162 (= N168), D226 (= D232), S230 (= S236), M231 (= M237)

4a8hA Crystal structure of putrescine transcarbamylase from enterococcus faecalis with n-(phosphonoacetyl)-putrescine (see paper)
37% identity, 98% coverage: 8:334/334 of query aligns to 3:311/340 of 4a8hA

query
sites
4a8hA

R

R

S

D

L

Y

L

V

T

T

V

T

Q

E

D

T

Y

Y

T

T

P

K

R

E

E

E

F

M

R

H

Y

Y

L

L

V

V

D

D

L

L

A

S

R

L

D

K

L

I

K

K

R

E

A

A

K

I

Y

K

A

N

R

G

T

Y

E

Y

Q

P

E

Q

H

L

L

L

K

K

G

N

K

K

E

S

I

L

C

G

L

M

I

I

F

F

E

Q

K

Q

T

S

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

V

A

S

F

F

E

E

V

T

A

A

C

M

S

E

D

Q

Q

L

G

G

A

G

N

H

V

G

T

E

Y

Y

L

L

D

A

P

P

A

G

G

Q

S

I

Q

Q

I

L

G

G

H
x
G

K

H

E

E

S

T

F

I

K

E

D

D

T

T

A

S

R

R

V

V

L

L

G

S

R

R

M

L

Y

V

D

D

A

I

I

L

E

M

Y

A

R
|
R

G

V

A

E

S

R

Q

H

A

H

G

S

V

I

E

V

T

D

L

L

A

A

K

N

Y

C

A

A

G

T

V

I

P

P

V

V

Y

I

N

N

G

G

L
x
M

T

S

D

D

E

Y

Y

N

H
|
H

P

P

T

T

Q
|
Q

M

E

I

L

A

G

D

D

V

L

M

C

T

T

M

M

R

V

E

E

H

H

-

L

-

P

S

E

D

G

K

K

P

K

I

L

S

E

E

D

I

C

K

K

Y

V

A

V

Y

F

I

V

G

G

D

D

T

A

R

-

S

T

N
x
Q

M

V

G

C

H

F

S

S

L

L

L

G

I

L

V

I

G

T

C

T

L

K

M

M

G

G

M

M

D

N

V

F

R

V

I

H

C

F

G

G

P

P

R

E

S

G

L

F

W

Q

P

L

S

N

E

E

Y

H

Q

Q

T

A

-

K

I

L

A

A

K

K

E

N

L

C

K

E

A

V

Q

-

S

S

G

G

A

G

R

S

L

F

M

L

I

V

T

T

D

D

P

A

K

-

A

S

V

V

E

E

G

G

V

A

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

Y

S

G

M

L

G

Y

E

E

P

A

K

E

E

L

V

S

W

E

K

E

E

E

R

R

I

M

Q

K

L

V

L

F

T

Y

P

P

-

K

Y

Y

Q

Q

V

V

N

N

A

Q

E

E

L

M

M

M

A

D

A

R

S

A

G

G

N

-

P

A

Q

N

T

C

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

Y

-

H

-

D

-

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

T

N

R

G

G

L

E

E

E

V

V

T

T

D

D

E

E

V

V

F

I

E

D

S

G

Q

K

A

N

N

S

V

I

A

C

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

L

H

T

T

S

I

I

K

R

A

G

L

L

V

V

A

Y

T

L

L

M

G

N

D

D

active site: R54 (= R59), T55 (= T60), G82 (≠ H87), R103 (= R108), H130 (= H135), Q133 (= Q138), D227 (= D232), C269 (= C274), R297 (= R320)
binding n-(phosphonoacetyl)-putrescine: S52 (= S57), T53 (= T58), R54 (= R59), T55 (= T60), R103 (= R108), M125 (≠ L130), H130 (= H135), Q164 (≠ N168), D227 (= D232), C269 (= C274), L270 (= L275), R297 (= R320)

Q837U7 Putrescine carbamoyltransferase; PTC; PTCase; Agmatine catabolism protein B; Putrescine transcarbamoylase; Putrescine transcarbamylase; EC 2.1.3.6 from Enterococcus faecalis (strain ATCC 700802 / V583) (see paper)
37% identity, 98% coverage: 8:334/334 of query aligns to 3:311/339 of Q837U7

query
sites
Q837U7

R

R

S

D

L

Y

L

V

T

T

V

T

Q

E

D

T

Y

Y

T

T

P

K

R

E

E

E

F

M

R

H

Y

Y

L

L

V

V

D

D

L

L

A

S

R

L

D

K

L

I

K

K

R

E

A

A

K

I

Y

K

A

N

R

G

T

Y

E

Y

Q

P

E

Q

H

L

L

L

K

K

G

N

K

K

E

S

I

L

C

G

L

M

I

I

F

F

E

Q

K

Q

T

S

S

S

T

T

R
|
R

T

T

R

R

C

V

A

S

F

F

E

E

V

T

A

A

C

M

S

E

D

Q

Q

L

G

G

A

G

N

H

V

G

T

E

Y

Y

L

L

D

A

P

P

A

G

G

Q

S

I

Q

Q

I

L

G

G

H

G

K

H

E

E

S

T

F

I

K

E

D

D

T

T

A

S

R

R

V

V

L

L

G

S

R

R

M

L

Y

V

D

D

A

I

I

L

E

M

Y

A

R

R

G

V

A

E

S

R

Q

H

A

H

G

S

V

I

E

V

T

D

L

L

A

A

K

N

Y

C

A

A

G

T

V

I

P

P

V

V

Y

I

N

N

G

G

L

M

T

S

D

D

E

Y

Y

N

H

H

P

P

T

T

Q

Q

M

E

I

L

A

G

D

D

V

L

M

C

T

T

M

M

R

V

E

E

H

H

-

L

-

P

S

E

D

G

K

K

P

K

I

L

S

E

E

D

I

C

K

K

Y

V

A

V

Y

F

I

V

G

G

D

D

T

A

R

-

S

T

N

Q

M

V

G

C

H

F

S

S

L

L

L

G

I

L

V

I

G

T

C

T

L

K

M

M

G

G

M

M

D

N

V

F

R

V

I

H

C

F

G

G

P

P

R

E

S

G

L

F

W

Q

P

L

S

N

E

E

Y

H

Q

Q

T

A

-

K

I

L

A

A

K

K

E

N

L

C

K

E

A

V

Q

-

S

S

G

G

A

G

R

S

L

F

M

L

I

V

T

T

D

D

P

A

K

-

A

S

V

V

E

E

G

G

V

A

D

D

F

F

I

L

H

Y

T

T

D

D

V

V

W

W

V
x
Y

S
x
G

M
x
L

G
x
Y

E

E

P

A

K

E

E

L

V

S

W

E

K

E

E

E

R

R

I

M

Q

K

L

V

L

F

T

Y

P

P

-

K

Y

Y

Q

Q

V

V

N

N

A

Q

E

E

L

M

M

M

A

D

A

R

S

A

G

G

N

-

P

A

Q

N

T

C

K

K

F

F

M

M

H

H

C

C

L

L

P

P

A

A

Y

-

H

-

D

-

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

T

N

R

G

G

L

E

E

E

V

V

T

T

D

D

E

E

V

V

F

I

E

D

S

G

Q

K

A

N

N

S

V

I

A

C

F

F

E

D

Q

E

A

A

E

E

N

N

R

R

M

L

H

T

T

S

I

I

K

R

A

G

L

L

V

V

A

Y

T

L

L

M

G

N

D

D

R54 (= R59) mutation to G: Loss of activity with putrescine and ornithine.
YGLY 230:233 (≠ VSMG 235:238) mutation to VSMG: Loss of activity with putrescine; increased activity with ornithine.

4am8D Crystal structure of the r54g mutant of putrescine transcarbamylase from enterococcus faecalis bound to a curing guanidinium ion
37% identity, 98% coverage: 8:334/334 of query aligns to 3:311/349 of 4am8D

query
sites
4am8D

R

R

S

D

L

Y

L

V

T

T

V

T

Q

E

D

T

Y

Y

T

T

P

K

R

E

E

E

F

M

R

H

Y

Y

L

L

V

V

D

D

L

L

A

S

R

L

D

K

L

I

K

K

R

E

A

A

K

I

Y

K

A

N

R

G

T

Y

E

Y

Q

P

E

Q

H

L

L

L

K

K

G

N

K

K

E

S

I

L

C

G

L

M

I

I

F

F

E

Q

K

Q

T

S

S
|
S

T
|
T

R
x
G

T
|
T

R

R

C

V

A

S

F

F

E

E

V

T

A

A

C

M

S

E

D

Q

Q

L

G

G

A

G

N

H

V

G

T

E

Y

Y

L

L

D

A

P

P

A

G

G

Q

S

I

Q

Q

I

L

G

G

H

G

K

H

E

E

S

T

F

I

K

E

D

D

T

T

A

S

R

R

V

V

L

L

G

S

R

R

M

L

Y

V

D

D

A

I

I

L

E

M

Y

A

R
|
R

G

V

A

E

S

R

Q

H

A

H

G

S

V

I

E

V

T

D

L

L

A

A

K

N

Y

C

A

A

G

T

V

I

P

P

V

V

Y

I

N

N

G

G

L
x
M

T

S

D

D

E

Y

Y

N

H
|
H

P

P

T

T

Q
|
Q

M

E

I

L

A

G

D

D

V

L

M

C

T

T

M

M

R

V

E

E

H

H

-

L

-

P

S

E

D

G

K

K

P

K

I

L

S

E

E

D

I

C

K

K

Y

V

A

V

Y

F

I

V

G

G

D

D

T

A

R

-

S

T

N
x
Q

M

V

G

C

H

F

S

S

L

L

L

G

I

L

V

I

G

T

C

T

L

K

M

M

G

G

M

M

D

N

V

F

R

V

I

H

C

F

G

G

P

P

R

E

S

G

L

F

W

Q

P

L

S

N

E

E

Y

H

Q

Q

T

A

-

K

I

L

A

A

K

K

E

N

L

C

K

E

A

V

Q

-

S

S

G

G

A

G

R

S

L

F

M

L

I

V

T

T

D

D

P

A

K

-

A

S

V

V

E

E

G

G

V

A

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

Y

S

G

M

L

G

Y

E

E

P

A

K

E

E

L

V

S

W

E

K

E

E

E

R

R

I

M

Q

K

L

V

L

F

T

Y

P

P

-

K

Y

Y

Q

Q

V

V

N

N

A

Q

E

E

L

M

M

M

A

D

A

R

S

A

G

G

N

-

P

A

Q

N

T

C

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

Y

-

H

-

D

-

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

T

N

R

G

G

L

E

E

E

V

V

T

T

D

D

E

E

V

V

F

I

E

D

S

G

Q

K

A

N

N

S

V

I

A

C

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

L

H

T

T

S

I

I

K

R

A

G

L

L

V

V

A

Y

T

L

L

M

G

N

D

D

active site: G54 (≠ R59), T55 (= T60), R103 (= R108), H130 (= H135), Q133 (= Q138), D227 (= D232), C269 (= C274), R297 (= R320)
binding n-(phosphonoacetyl)-l-ornithine: S52 (= S57), T53 (= T58), G54 (≠ R59), T55 (= T60), R103 (= R108), M125 (≠ L130), H130 (= H135), Q164 (≠ N168), D227 (= D232), L270 (= L275), R297 (= R320)

4am8A Crystal structure of the r54g mutant of putrescine transcarbamylase from enterococcus faecalis bound to a curing guanidinium ion
37% identity, 98% coverage: 8:334/334 of query aligns to 3:311/336 of 4am8A

query
sites
4am8A

R

R

S

D

L

Y

L

V

T

T

V

T

Q

E

D

T

Y

Y

T

T

P

K

R

E

E

E

F

M

R

H

Y

Y

L

L

V

V

D

D

L

L

A

S

R

L

D

K

L

I

K

K

R

E

A

A

K

I

Y

K

A

N

R

G

T

Y

E

Y

Q

P

E

Q

H

L

L

L

K

K

G

N

K

K

E

S

I

L

C

G

L

M

I

I

F

F

E

Q

K

Q

T

S

S
|
S

T
|
T

R
x
G

T
|
T

R

R

C

V

A

S

F

F

E

E

V

T

A

A

C

M

S

E

D

Q

Q

L

G

G

A

G

N

H

V

G

T

E

Y

Y

L

L

D

A

P

P

A

G

G

Q

S

I

Q

Q

I

L

G

G

H

G

K

H

E

E

S

T

F

I

K

E

D

D

T

T

A

S

R

R

V

V

L

L

G

S

R

R

M

L

Y

V

D

D

A

I

I

L

E

M

Y

A

R
|
R

G

V

A

E

S

R

Q

H

A

H

G

S

V

I

E

V

T

D

L

L

A

A

K

N

Y

C

A

A

G

T

V

I

P

P

V

V

Y

I

N

N

G

G

L
x
M

T

S

D

D

E

Y

Y

N

H
|
H

P

P

T

T

Q
|
Q

M

E

I

L

A

G

D

D

V

L

M

C

T

T

M

M

R

V

E

E

H

H

-

L

-

P

S

E

D

G

K

K

P

K

I

L

S

E

E

D

I

C

K

K

Y

V

A

V

Y

F

I

V

G

G

D

D

T

A

R

-

S

T

N
x
Q

M

V

G

C

H

F

S

S

L

L

L

G

I

L

V

I

G

T

C

T

L

K

M

M

G

G

M

M

D

N

V

F

R

V

I

H

C

F

G

G

P

P

R

E

S

G

L

F

W

Q

P

L

S

N

E

E

Y

H

Q

Q

T

A

-

K

I

L

A

A

K

K

E

N

L

C

K

E

A

V

Q

-

S

S

G

G

A

G

R

S

L

F

M

L

I

V

T

T

D

D

P

A

K

-

A

S

V

V

E

E

G

G

V

A

D

D

F

F

I

L

H

Y

T

T

D
|
D

V

V

W

W

V

Y

S

G

M

L

G

Y

E

E

P

A

K

E

E

L

V

S

W

E

K

E

E

E

R

R

I

M

Q

K

L

V

L

F

T

Y

P

P

-

K

Y

Y

Q

Q

V

V

N

N

A

Q

E

E

L

M

M

M

A

D

A

R

S

A

G

G

N

-

P

A

Q

N

T

C

K

K

F

F

M

M

H

H

C
|
C

L
|
L

P

P

A

A

Y

-

H

-

D

-

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

T

N

R

G

G

L

E

E

E

V

V

T

T

D

D

E

E

V

V

F

I

E

D

S

G

Q

K

A

N

N

S

V

I

A

C

F

F

E

D

Q

E

A

A

E

E

N

N

R
|
R

M

L

H

T

T

S

I

I

K

R

A

G

L

L

V

V

A

Y

T

L

L

M

G

N

D

D

active site: G54 (≠ R59), T55 (= T60), R103 (= R108), H130 (= H135), Q133 (= Q138), D227 (= D232), C269 (= C274), R297 (= R320)
binding n-(phosphonoacetyl)-l-ornithine: S52 (= S57), T53 (= T58), G54 (≠ R59), T55 (= T60), R103 (= R108), M125 (≠ L130), H130 (= H135), Q164 (≠ N168), D227 (= D232), L270 (= L275), R297 (= R320)

Sites not aligning to the query:

binding glutamine: 333, 336

P00480 Ornithine transcarbamylase, mitochondrial; OTCase; Ornithine carbamoyltransferase, mitochondrial; EC 2.1.3.3 from Homo sapiens (Human) (see 31 papers)
35% identity, 99% coverage: 5:334/334 of query aligns to 37:344/354 of P00480

query
sites
P00480

L

L

R

K

N
x
G

R
|
R

S

D

L

L

L
|
L

T

T

V

L

Q
x
K

D

N

Y

F

T

T

P

G

R

E

E

E

F

I

R

K

Y
|
Y

L

M

V

L

D

W

L

L

A

S

R

A

D

D

L
|
L

K

K

-

F

R

R

A

I

K

K

Y

Q

A

K

R

G

T

E

E

Y

Q

L

E

P

H

L

L

L

K

Q

G

G

K

K

E

S

I

L

C

G

L

M

I

I

F

F

E

E

K
|
K

T

R

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

L

A

S

F

T

E

E

V

T

A
x
G

C
x
F

S

A

D

L

Q

L

G

G

A

G

N

H

V

P

T

C

Y

F

L
|
L

D

T

P

T

A

Q

G

D

S

I

Q

H

I

L

G

G

H

V

K

N

E

E

S

S

F

L

K
x
T

D
|
D

T

T

A

A

R
|
R

V

V

L

L

G

S

R

S

M

M

Y

A

D

D

A

A

I

V

E

L

Y
x
A

R
|
R

G

V

A

Y

S

K

Q

Q

A

S

G

D

V

L

E

D

T

T

L

L

A

A

K

K

Y

E

A

A

G

S

V

I

P

P

V

I

Y

I

N

N

G

G

L

L

T

S

D

D

E

L

Y

Y

H
|
H

P

P

T

I

Q
|
Q

M
x
I

I

L

A

A

D

D

V
x
Y

M

L

T
|
T

M
x
L

R

Q

E

E

H

H

S
x
Y

D

S

K

S

P

-

I

L

S

K

E
x
G

I

L

K

T

Y

L

A

S

Y

W

I

I

G
|
G

D
|
D

T

G

R

-

S

N

N

N

M

I

G
x
L

H

H

S

S

L

I

L

M

I

M

V
x
S

G

A

C
x
A

L

K

M

F

G

G

M
|
M

D
x
H

V

L

R

Q

I

A

C

A

G

T

P
|
P

R

K

S

G

L

Y

W

E

P
|
P

S

D

E

A

E

S

Y

V

Q

T

T

K

I

L

A

A

K

E

E

Q

L

Y

K

A

A

K

Q

E

S

N

G

G

A

T

R

K

L
|
L

M

L

I

L

T

T

D

N

D

D

P

P

K

L

A

E

A

A

V

A

E

H

G

G

V

G

D

N

F

V

I

L

H

I

T
|
T

D

D

V
x
T

W
|
W

V

I

S

S

M

M

G
|
G

E
x
Q

P

E

K
x
E

E

E

V

K

W

-

K

K

E

K

R
|
R

I

L

Q

Q

L

A

L

F

T

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

A

K

A

V

S

A

G

A

N

S

P

D

Q

W

T

T

K

-

F

F

M

L

H
|
H

C
|
C

L
|
L

P

P

A

R

Y

K

H

P

D
x
E

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

-

N

-

G

-

L

-

E

E

V

V

T

D

D

D

E

E

V

V

F

F

E

Y

S

S

Q

P

A

R

N

S

V

L

A

V

F

F

E

P

Q

E

A

A

E

E

N

N

R
|
R

M

K

H

W

T
|
T

I

I

K

M

A

A

L

V

L

M

V

V

A
x
S

T

L

L

L

G
x
T

D

D

G39 (≠ N7) to C: in OTCD; late onset; dbSNP:rs72554306
R40 (= R8) to H: in OTCD; late onset; dbSNP:rs72554308
L43 (= L11) to F: in dbSNP:rs72554309
K46 (≠ Q14) to R: in dbSNP:rs1800321
Y55 (= Y23) to D: in OTCD; late onset; dbSNP:rs72554319
L63 (= L31) to P: in OTCD; late onset; dbSNP:rs72554324
K88 (= K55) modified: N6-acetyllysine; alternate; to N: in OTCD; late onset; dbSNP:rs72554339
STRT 90:93 (= STRT 57:60) binding carbamoyl phosphate
G100 (≠ A67) to D: in OTCD; late onset; dbSNP:rs72554349
F101 (≠ C68) to L: in dbSNP:rs1133135
L111 (= L78) to P: in dbSNP:rs1800324
T125 (≠ K92) to M: in OTCD; neonatal; dbSNP:rs72554356
D126 (= D93) to G: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; 0.9% of wild-type activity; dbSNP:rs72554358
R129 (= R96) to H: in OTCD; early onset; decreased ornithine carbamoyltransferase activity; 2.1% of wild-type activity; dbSNP:rs66656800
A140 (≠ Y107) to P: in OTCD; late onset; dbSNP:rs72556260
R141 (= R108) binding carbamoyl phosphate; to Q: in OTCD; most common variant; loss of ornithine carbamoyltransferase activity; activity is 100-fold lower; dbSNP:rs68026851
H168 (= H135) binding carbamoyl phosphate
Q171 (= Q138) binding carbamoyl phosphate
I172 (≠ M139) to M: in OTCD; early onset; loss of ornithine carbamoyltransferase activity; dbSNP:rs72556280
Y176 (≠ V143) to C: in OTCD; late onset; dbSNP:rs72556283
TL 178:179 (≠ TM 145:146) natural variant: Missing (in OTCD; neonatal)
Y183 (≠ S150) to D: in OTCD; late onset; dbSNP:rs72556292
G188 (≠ E156) to R: in OTCD; neonatal; dbSNP:rs72556294
G195 (= G163) to R: in OTCD; loss of ornithine carbamoyltransferase activity; dbSNP:rs67294955
D196 (= D164) to V: in OTCD; neonatal; decreased ornithine carbamoyltransferase activity; 3.7% activity; dbSNP:rs72556300
L201 (≠ G170) to P: in OTCD; neonatal; dbSNP:rs72558407
S207 (≠ V176) to R: in OTCD; neonatal; dbSNP:rs72558415
A209 (≠ C178) to V: in OTCD; neonatal; dbSNP:rs72558417
M213 (= M182) to K: in OTCD; late onset
H214 (≠ D183) to Y: in OTCD; neonatal; dbSNP:rs72558420
P220 (= P189) to A: in OTCD; late onset; dbSNP:rs72558425
P225 (= P194) to T: in OTCD; late onset; dbSNP:rs72558428
L244 (= L213) to Q: in OTCD; late onset; dbSNP:rs72558436
T262 (= T231) to K: in OTCD; mild; dbSNP:rs67333670
T264 (≠ V233) to A: in OTCD; late onset; decreased ornithine carbamoyltransferase activity; 8.9% activity; dbSNP:rs72558444; to I: in OTCD; late onset; dbSNP:rs67156896
W265 (= W234) to L: in OTCD; mild; dbSNP:rs72558446
G269 (= G238) to E: in OTCD; neonatal; dbSNP:rs72558450
Q270 (≠ E239) to R: in dbSNP:rs1800328
E272 (≠ K241) natural variant: Missing (in OTCD; late onset; dbSNP:rs72558452)
R277 (= R247) to Q: in OTCD; late onset; dbSNP:rs66724222; to W: in OTCD; late onset; dbSNP:rs72558454
H302 (= H273) to L: in OTCD; female; late onset; dbSNP:rs67993095; to Y: in OTCD; neonatal; dbSNP:rs72558463
C303 (= C274) to R: in OTCD; neonatal; dbSNP:rs67468335
CL 303:304 (= CL 274:275) binding carbamoyl phosphate
E309 (≠ D280) natural variant: Missing (in OTCD; late onset)
R330 (= R320) binding carbamoyl phosphate
T333 (= T323) natural variant: T -> A
S340 (≠ A330) to P: in OTCD; late onset; dbSNP:rs72558489
T343 (≠ G333) to K: in OTCD; late onset; dbSNP:rs72558491

Sites not aligning to the query:

1:32 modified: transit peptide, Mitochondrion
15 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-23 and G-26.
23 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-26.
26 R→G: Loss of cleavage of the transit peptide and loss of localization to mitochondrial matrix; when associated with G-15 and G-23.

1othA Crystal structure of human ornithine transcarbamoylase complexed with n-phosphonacetyl-l-ornithine (see paper)
35% identity, 99% coverage: 4:334/334 of query aligns to 3:311/321 of 1othA

query
sites
1othA

N

Q

L

L

R

K

N

G

R

R

S

D

L

L

T

T

V

L

Q

K

D

N

Y

F

T

T

P

G

R

E

E

E

F

I

R

K

Y

Y

L

M

V

L

D

W

L

L

A

S

R

A

D

D

L

L

K

K

-

F

R

R

A

I

K

K

Y

Q

A

K

R

G

T

E

E

Y

Q

L

E

P

H

L

L

L

K

Q

G

G

K

K

E

S

I

L

C

G

L

M

I

I

F

F

E

E

K

K

T

R

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

L

A

S

F

T

E

E

V

T

A

G

C

F

S

A

D

L

Q

L

G

G

A

G

N

H

V

P

T

C

Y

F

L

L

D

T

P

T

A

Q

G

D

S

I

Q

H

I

L

G

G

H
x
V

K

N

E

E

S

S

F

L

K

T

D

D

T

T

A

A

R

R

V

V

L

L

G

S

R

S

M

M

Y

A

D

D

A

A

I

V

E

L

Y

A

R
|
R

G

V

A

Y

S

K

Q

Q

A

S

G

D

V

L

E

D

T

T

L

L

A

A

K

K

Y

E

A

A

G

S

V

I

P

P

V

I

Y

I

N

N

G

G

L
|
L

T

S

D

D

E

L

Y

Y

H
|
H

P

P

T

I

Q
|
Q

M

I

I

L

A

A

D

D

V

Y

M

L

T

T

M

L

R

Q

E

E

H

H

S

Y

D

S

K

S

P

-

I

L

S

K

E

G

I

L

K

T

Y

L

A

S

Y

W

I

I

G

G

D

D

T

G

R

-

S

N

N
|
N

M

I

G

L

H

H

S

S

L

I

L

M

I

M

V

S

G

A

C

A

L

K

M

F

G

G

M

M

D

H

V

L

R

Q

I

A

C

A

G

T

P

P

R

K

S

G

L

Y

W

E

P

P

S

D

E

A

E

S

Y

V

Q

T

T

K

I

L

A

A

K

E

E

Q

L

Y

K

A

A

K

Q

E

S

N

G

G

A

T

R

K

L

L

M

L

I

L

T

T

D

N

D

D

P

P

K

L

A

E

A

A

V

A

E

H

G

G

V

G

D

N

F

V

I

L

H

I

T

T

D
|
D

V

T

W

W

V

I

S
|
S

M
|
M

G

G

E

R

P

E

K

E

E

E

V

K

W

-

K

K

E

K

R

R

I

L

Q

Q

L

A

L

F

T

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

A

K

A

V

S

A

G

A

N

S

P

D

Q

W

T

T

K

-

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

R

Y

K

H

P

D

E

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

-

N

-

G

-

L

-

E

E

V

V

T

D

D

D

E

E

V

V

F

F

E

Y

S

S

Q

P

A

R

N

S

V

L

A

V

F

F

E

P

Q

E

A

A

E

E

N

N

R
|
R

M

K

H

W

T

T

I

I

K

M

A

A

L

V

L

M

V

V

A

S

T

L

L

L

G

T

D

D

active site: R59 (= R59), T60 (= T60), V87 (≠ H87), R108 (= R108), H135 (= H135), Q138 (= Q138), D230 (= D232), C270 (= C274), R297 (= R320)
binding n-(phosphonoacetyl)-l-ornithine: S57 (= S57), T58 (= T58), R59 (= R59), T60 (= T60), R108 (= R108), L130 (= L130), H135 (= H135), N166 (= N168), D230 (= D232), S234 (= S236), M235 (= M237), C270 (= C274), L271 (= L275), R297 (= R320)

1c9yA Human ornithine transcarbamylase: crystallographic insights into substrate recognition and catalytic mechanism (see paper)
35% identity, 99% coverage: 4:334/334 of query aligns to 3:311/321 of 1c9yA

query
sites
1c9yA

N

Q

L

L

R

K

N

G

R

R

S

D

L

L

T

T

V

L

Q

K

D

N

Y

F

T

T

P

G

R

E

E

E

F

I

R

K

Y

Y

L

M

V

L

D

W

L

L

A

S

R

A

D

D

L

L

K

K

-

F

R

R

A

I

K

K

Y

Q

A

K

R

G

T

E

E

Y

Q

L

E

P

H

L

L

L

K

Q

G

G

K

K

E

S

I

L

C

G

L

M

I

I

F

F

E

E

K

K

T

R

S
|
S

T
|
T

R
|
R

T
|
T

R

R

C

L

A

S

F

T

E

E

V

T

A

G

C

F

S

A

D

L

Q

L

G

G

A

G

N

H

V

P

T

C

Y

F

L

L

D

T

P

T

A

Q

G

D

S

I

Q

H

I

L

G

G

H
x
V

K

N

E

E

S

S

F

L

K

T

D

D

T

T

A

A

R

R

V

V

L

L

G

S

R

S

M

M

Y

A

D

D

A

A

I

V

E

L

Y

A

R
|
R

G

V

A

Y

S

K

Q

Q

A

S

G

D

V

L

E

D

T

T

L

L

A

A

K

K

Y

E

A

A

G

S

V

I

P

P

V

I

Y

I

N

N

G

G

L
|
L

T

S

D

D

E

L

Y

Y

H
|
H

P

P

T

I

Q
|
Q

M

I

I

L

A

A

D

D

V

Y

M

L

T

T

M

L

R

Q

E

E

H

H

S

Y

D

S

K

S

P

-

I

L

S

K

E

G

I

L

K

T

Y

L

A

S

Y

W

I

I

G

G

D

D

T

G

R

-

S

N

N
|
N

M

I

G

L

H

H

S

S

L

I

L

M

I

M

V

S

G

A

C

A

L

K

M

F

G

G

M

M

D

H

V

L

R

Q

I

A

C

A

G

T

P

P

R

K

S

G

L

Y

W

E

P

P

S

D

E

A

E

S

Y

V

Q

T

T

K

I

L

A

A

K

E

E

Q

L

Y

K

A

A

K

Q

E

S

N

G

G

A

T

R

K

L

L

M

L

I

L

T

T

D

N

D

D

P

P

K

L

A

E

A

A

V

A

E

H

G

G

V

G

D

N

F

V

I

L

H

I

T

T

D
|
D

V

T

W

W

V

I

S
|
S

M
|
M

G

G

E

R

P

E

K

E

E

E

V

K

W

-

K

K

E

K

R

R

I

L

Q

Q

L

A

L

F

T

Q

P

G

Y

Y

Q

Q

V

V

N

T

A

M

E

K

L

T

M

A

A

K

A

V

S

A

G

A

N

S

P

D

Q

W

T

T

K

-

F

F

M

L

H

H

C
|
C

L
|
L

P

P

A

R

Y

K

H

P

D

E

T

-

E

-

T

-

A

-

I

-

G

-

K

-

Q

-

I

-

S

-

E

-

D

-

Y

-

G

-

M

-

S

-

N

-

G

-

L

-

E

E

V

V

T

D

D

D

E

E

V

V

F

F

E

Y

S

S

Q

P

A

R

N

S

V

L

A

V

F

F

E

P

Q

E

A

A

E

E

N

N

R
|
R

M

K

H

W

T

T

I

I

K

M

A

A

L

V

L

M

V

V

A

S

T

L

L

L

G

T

D

D

active site: R59 (= R59), T60 (= T60), V87 (≠ H87), R108 (= R108), H135 (= H135), Q138 (= Q138), D230 (= D232), C270 (= C274), R297 (= R320)
binding phosphoric acid mono(formamide)ester: S57 (= S57), T58 (= T58), R59 (= R59), T60 (= T60), R108 (= R108), C270 (= C274), L271 (= L275), R297 (= R320)
binding norvaline: L130 (= L130), N166 (= N168), D230 (= D232), S234 (= S236), M235 (= M237)

Query Sequence

>WP_011970089.1 NCBI__GCF_000017145.1:WP_011970089.1
MSFNLRNRSLLTVQDYTPREFRYLVDLARDLKRAKYARTEQEHLKGKEICLIFEKTSTRT
RCAFEVACSDQGANVTYLDPAGSQIGHKESFKDTARVLGRMYDAIEYRGASQAGVETLAK
YAGVPVYNGLTDEYHPTQMIADVMTMREHSDKPISEIKYAYIGDTRSNMGHSLLIVGCLM
GMDVRICGPRSLWPSEEYQTIAKELKAQSGARLMITDDPKAAVEGVDFIHTDVWVSMGEP
KEVWKERIQLLTPYQVNAELMAASGNPQTKFMHCLPAYHDTETAIGKQISEDYGMSNGLE
VTDEVFESQANVAFEQAENRMHTIKALLVATLGD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory