SitesBLAST
Comparing WP_011973151.1 NCBI__GCF_000017185.1:WP_011973151.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
1wqaA Crystal structure of pyrococcus horikoshii phosphomannomutase/phosphoglucomutase complexed with mg2+
38% identity, 99% coverage: 2:449/452 of query aligns to 3:455/455 of 1wqaA
- active site: R11 (= R10), S101 (= S88), H102 (= H89), K111 (= K98), D243 (= D234), D245 (= D236), D247 (= D238), R248 (= R239), G330 (≠ T322), R340 (≠ P332)
- binding magnesium ion: S101 (= S88), D243 (= D234), D245 (= D236), D247 (= D238)
7omlA Bacillus subtilis phosphoglucomutase glmm (metal bound) (see paper)
32% identity, 98% coverage: 2:445/452 of query aligns to 2:441/445 of 7omlA
7ojrA Bacillus subtilis phosphoglucomutase glmm (phosphate bound) (see paper)
32% identity, 98% coverage: 2:445/452 of query aligns to 2:441/445 of 7ojrA
3i3wA Structure of a phosphoglucosamine mutase from francisella tularensis
33% identity, 99% coverage: 2:448/452 of query aligns to 1:440/441 of 3i3wA
- active site: R9 (= R10), S99 (= S88), H100 (= H89), K109 (= K98), D237 (= D234), D239 (= D236), D241 (= D238), R242 (= R239), H324 (≠ T322)
- binding zinc ion: S99 (= S88), D237 (= D234), D239 (= D236), D241 (= D238)
4il8A Crystal structure of an h329a mutant of p. Aeruginosa pmm/pgm (see paper)
29% identity, 95% coverage: 1:430/452 of query aligns to 10:434/459 of 4il8A
- active site: R16 (≠ S7), S104 (= S88), H105 (= H89), K114 (= K98), D238 (= D234), D240 (= D236), D242 (= D238), R243 (= R239), A325 (≠ T322), D336 (≠ P332)
- binding magnesium ion: S104 (= S88), D238 (= D234), D240 (= D236), D242 (= D238)
P26276 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see 10 papers)
29% identity, 95% coverage: 1:430/452 of query aligns to 14:438/463 of P26276
- R15 (≠ K2) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 25-fold.
- Y17 (≠ F4) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
- R20 (≠ S7) mutation to A: No phosphoglucomutase activity.
- S108 (= S88) binding via phosphate group; modified: Phosphoserine; mutation S->A,V: About 5% activity, still subject to substrate inhibition and requires G1,6P as an activator; phosphorylation occurs at a different site.; mutation to C: KM for G1P unchanged, kcat decreases 24-fold; G1,6P stimulates reaction by 2-3 orders of magnitude. No stable protein phosphorylation detected, altered ligation of metal residue.
- N110 (= N90) mutation to A: KM halves, decreases processivity as dissociation of G1,6P intermediate increases 30-fold.
- D242 (= D234) binding Mg(2+)
- D244 (= D236) binding Mg(2+)
- D246 (= D238) binding Mg(2+)
- R247 (= R239) mutation to A: Small reduction in KM, small increase in dissociation of G1,6P intermediate.
- R262 (≠ K254) mutation to A: Increases KM 2-fold, decreases kcat 9-fold for G1P. Alters flexibility of the hinge region.
- K285 (≠ D277) binding alpha-D-glucose 1-phosphate
- H308 (≠ D300) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
- E325 (= E318) mutation to A: Reduces KM and Vmax approximately 2-fold.
- EMSGH 325:329 (≠ EPSGT 318:322) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
- H329 (≠ T322) mutation to A: No phosphoglucomutase activity using G1P as substrate, protein is less easily phosphorylated, no significant change in structure.
- P368 (= P360) mutation to G: Increases KM 2-fold, decreases kcat 6-fold for G1P. Alters flexibility of the hinge region, structure is less compact.
- R421 (= R413) mutation to C: Loss of phosphomannomutase activity, very low phosphoglucomutase activity.
- RASNT 421:425 (≠ RPSGT 413:417) binding alpha-D-glucose 1-phosphate; binding alpha-D-mannose 1-phosphate
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
Q02E40 Phosphomannomutase/phosphoglucomutase; PMM / PGM; EC 5.4.2.2; EC 5.4.2.8 from Pseudomonas aeruginosa (strain UCBPP-PA14) (see paper)
29% identity, 95% coverage: 1:430/452 of query aligns to 14:438/463 of Q02E40
- S108 (= S88) active site, Non-phosphorylated intermediate; modified: Phosphoserine
2h5aX Complex of the enzyme pmm/pgm with xylose 1-phosphate (see paper)
29% identity, 95% coverage: 1:430/452 of query aligns to 6:430/455 of 2h5aX
- active site: H101 (= H89), D234 (= D234), D236 (= D236), D238 (= D238), R239 (= R239), D332 (≠ P332)
- binding 1-O-phosphono-alpha-D-xylopyranose: Y9 (≠ F4), T298 (≠ V298), G299 (= G299), H300 (≠ D300), E317 (= E318), S319 (= S320), H321 (≠ T322), R413 (= R413), S415 (= S415), N416 (≠ G416), T417 (= T417)
- binding zinc ion: S100 (= S88), D234 (= D234), D236 (= D236), D238 (= D238)
2h4lX Complex of pmm/pgm with ribose 1-phosphate (see paper)
29% identity, 95% coverage: 1:430/452 of query aligns to 6:430/455 of 2h4lX
- active site: H101 (= H89), D234 (= D234), D236 (= D236), D238 (= D238), R239 (= R239), D332 (≠ P332)
- binding 1-O-phosphono-alpha-D-ribofuranose: Y9 (≠ F4), R12 (≠ S7), S100 (= S88), T298 (≠ V298), E317 (= E318), R413 (= R413), S415 (= S415), N416 (≠ G416), T417 (= T417)
- binding zinc ion: S100 (= S88), D234 (= D234), D236 (= D236), D238 (= D238)
2fkfA Phosphomannomutase/phosphoglucomutase from pseudomonas aeruginosa with alpha-d-glucose 1,6-bisphosphate bound (see paper)
29% identity, 95% coverage: 1:430/452 of query aligns to 6:430/455 of 2fkfA
- active site: R12 (≠ S7), S100 (= S88), H101 (= H89), K110 (= K98), D234 (= D234), D236 (= D236), D238 (= D238), R239 (= R239), H321 (≠ T322), D332 (≠ P332)
- binding 1,6-di-O-phosphono-alpha-D-glucopyranose: R7 (≠ K2), H101 (= H89), S319 (= S320), R413 (= R413), S415 (= S415), N416 (≠ G416), T417 (= T417)
- binding zinc ion: S100 (= S88), D234 (= D234), D236 (= D236), D238 (= D238)
1pcmX Enzyme-ligand complex of p. Aeruginosa pmm/pgm (see paper)
29% identity, 95% coverage: 1:430/452 of query aligns to 6:430/455 of 1pcmX