SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011973405.1 NCBI__GCF_000017185.1:WP_011973405.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

Q12640 Chorismate synthase aro-2; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 1.5.1.38; EC 4.2.3.5 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 3 papers)
38% identity, 100% coverage: 1:372/373 of query aligns to 1:400/432 of Q12640

query
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Q12640

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S16 (= S16) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 70-fold.
H17 (= H17) active site; mutation to A: Reduces the chorismate synthase activity 20-fold.
H106 (= H104) active site; mutation to A: Reduces the chorismate synthase activity 10-fold.
S127 (= S125) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 6-fold.
D367 (= D339) active site; mutation D->A,N: Does not affect substrate binding but stringly reduces the chorismate synthase activity.

1um0A Crystal structure of chorismate synthase complexed with fmn (see paper)
40% identity, 97% coverage: 1:363/373 of query aligns to 1:348/365 of 1um0A

query
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binding flavin mononucleotide: H104 (= H104), R123 (= R123), S125 (= S125), N241 (= N250), G242 (≠ A251), L280 (= L294), G281 (= G295), K296 (= K310), T298 (= T312), P299 (= P313), S300 (= S314), I327 (= I342), R330 (= R345)

P56122 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
40% identity, 97% coverage: 1:363/373 of query aligns to 1:348/365 of P56122

query
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P56122

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RSS 123:125 (= RSS 123:125) binding FMN
KPTPS 296:300 (= KPTPS 310:314) binding FMN

P9WPY1 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
33% identity, 94% coverage: 8:359/373 of query aligns to 2:363/401 of P9WPY1

query
sites
P9WPY1

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S

A

A

I

I

E

V

I

Y

G

N

N

T

T

N

E

Q

W

K

P

S

K

K

W

-

E

-

T

-

V

-

M

-

A

-

A

-

D

-

P

-

V

D

D

Y

P

S

A

H

E

L

L

K

A

N

D

T

V

-

A

-

R

-

N

-

A

-

P

-

L

-

T

-

R

P

P

R

R

P

P

G

G

H

H

A

A

D

D

L

Y

S

A

Y

G

K

M

L

L

K

K

Y

Y

G

G

N

F

Y

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

G

A

R

R

T

E

T

T

I

A

G

A

H

R

T

V

I

A

G

A

G

G

A

T

I

V

A

A

K

R

K

A

L

F

L

L

D

R

Y

Q

T

A

H

L

N

G

I

V

K

E

I

V

I

L

G

S

Y

H

T

V

T

I

K

S

I

I

G

G

N

-

I

-

E

-

G

-

D

-

F

-

N

-

Y

-

Y

-

N

A

S

S

I

A

E

P

N

Y

N

E

E

G

K

P

M

P

I

P

N

R

E

A

E

E

L

D

I

L

N

P

K

A

I

I

E

D

N

A

N

S

P

P

L

V

R

R

C

A

P

Y

S

D

S

K

N

A

A

A

D

E

-

A

E

D

M

M

K

I

D

A

F

Q

V

I

L

E

N

A

A

A

M

K

E

K

N

D

K

G

N

D

S

T

V

L

G

G

G

G

V

V

I

V

E

E

L

A

I

V

A

A

L

L

N

G

V

L

P

P

V

V

G

G

V

L

G

G

N

S

P

F

I

T

F

S

G

G

-

D

-

H

K

R

L

L

N

D

G

S

E

Q

L

L

S

A

N

A

A

A

I

V

M

M

N

G

I

I

N
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

R

D

G

G

F

F

E

Q

S

T

A

A

E

R

L

R

L

R

G

G

S

S

E

R

M

A

N

H

D

D

E

E

Y

M

Y

Y

Y

P

D

G

E

P

N

D

N

G

N

V

I

V

K

R

L

-

K

S

T

T

N

N

N

R

C

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

S

T

C

N

G

G

A

Q

P

P

L

L

V

R

I

V

R

R

V

A

A

A

V

M

K
|
K

P
|
P

T
x
I

P
x
S

S
x
T

I

V

S

P

A

R

V

A

Q

L

S

A

T

T

I

V

N

D

I

L

E

A

N

T

K

G

T

-

T

D

E

E

N

A

L

V

E

A

I

I

G

H

G

Q

R
|
R

H

S

D

D

P

V

I

C

I

A

V

V

P

P

R

A

V

A

I

G

P

V

V

V

L

V

E

E

S

T

M

M

V

V

A

A

I

L

A

V

L

L

S

A

R40 (= R46) binding NADP(+)
R46 (≠ L52) binding NADP(+)
QA 256:257 (≠ NA 250:251) binding FMN
KPIST 315:319 (≠ KPTPS 310:314) binding FMN
R341 (= R337) binding FMN

2qhfA Mycobacterium tuberculosis chorismate synthase in complex with nca
33% identity, 94% coverage: 8:359/373 of query aligns to 2:363/392 of 2qhfA

query
sites
2qhfA

F

L

R

R

I

W

T

I

A

T

W

A

G

G

E

E

S

S

H

H

G

G

K

R

A

A

L

L

G

V

A

A

V

V

V

V

D

E

G

G

C

M

P

V

S

A

N

G

L

V

P

H

L

V

T

T

G

S

E

A

D

D

I

I

Q

A

K

D

E

Q

L

L

N

A

R

R

R

R

R

R

P

L

G

G

Y

Y

S

G

L
x
R

F

G

S

A

T

R

P

M

R

T

K

F

E

E

G

R

D

D

K

A

V

V

E

T

I

V

L

L

S

S

G

G

I

I

F

R

E

H

G

G

K

S

T

T

T

L

G

G

T

G

P

P

I

I

S

A

A

I

I

E

V

I

Y

G

N

N

T

T

N

E

Q

W

K

P

S

K

K

W

-

E

-

T

-

V

-

M

-

A

-

A

-

D

-

P

-

V

D

D

Y

P

S

A

H

E

L

L

K

A

N

D

T

V

-

A

-

R

-

N

-

A

-

P

-

L

-

T

-

R

P

P

R

R

P

P

G

G

H

H

A

A

D

D

L

Y

S

A

Y

G

K

M

L

L

K

K

Y

Y

G

G

N

F

Y

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

G
x
A

R

R

T

E

T

T

I

A

G

A

H

R

T

V

I

A

G

A

G

G

A

T

I

V

A

A

K

R

K

A

L

F

L

L

D

R

Y

Q

T

A

H

L

N

G

I

V

K

E

I

V

I

L

G

S

Y

H

T

V

T

I

K

S

I

I

G

G

N

-

I

-

E

-

G

-

D

-

F

-

N

-

Y

-

Y

-

N

A

S

S

I

A

E

P

N

Y

N

E

E

G

K

P

M

P

I

P

N

R

E

A

E

E

L

D

I

L

N

P

K

A

I

I

E

D

N

A

N

S

P

P

L

V

R

R

C

A

P

Y

S

D

S

K

N

A

A

A

D

E

-

A

E

D

M

M

K

I

D

A

F

Q

V

I

L

E

N

A

A

A

M

K

E

K

N

D

K

G

N

D

S

T

V

L

G

G

G

G

V

V

I

V

E

E

L

A

I

V

A

A

L

L

N

G

V

L

P

P

V

V

G

G

V

L

G

G

N

S

P

F

I

T

F

S

G

G

-

D

-

H

K

R

L

L

N

D

G

S

E

Q

L

L

S

A

N

A

A

A

I

V

M

M

N

G

I
|
I

N
x
Q

A

A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

R

D

G

G

F

F

E

Q

S

T

A

A

E

R

L

R

L

R

G

G

S

S

E

R

M

A

N

H

D

D

E

E

Y

M

Y

Y

Y

P

D

G

E

P

N

D

N

G

N

V

I

V

K

R

L

-

K

S

T

T

N

N

N

R

C

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

S

T

C

N

G

G

A

Q

P

P

L

L

V

R

I

V

R

R

V

A

A

A

V
x
M

K

K

P

P

T
x
I

P

S

S

T

I

V

S

P

A

R

V

A

Q

L

S

A

T

T

I

V

N

D

I

L

E

A

N

T

K

G

T

-

T

D

E

E

N

A

L

V

E

A

I

I

G

H

G

Q

R
|
R

H

S

D

D

P

V

I

C

I

A

V

V

P

P

R

A

V

A

I

G

P

V

V

V

L

V

E

E

S

T

M

M

V

V

A

A

I

L

A

V

L

L

S

A

binding nicotinamide: R46 (≠ L52), A138 (≠ G126), I255 (= I249), Q256 (≠ N250), M314 (≠ V309), I317 (≠ T312), R341 (= R337)

2o12A Mycobacterium tuberculosis chorismate synthase in complex with fmn
33% identity, 94% coverage: 8:359/373 of query aligns to 2:357/386 of 2o12A

query
sites
2o12A

F

L

R

R

I

W

T

I

A

T

W

A

G

G

E

E

S

S

H

H

G

G

K

R

A

A

L

L

G

V

A

A

V

V

V

V

D

E

G

G

C

M

P

V

S

A

N

G

L

V

P

H

L

V

T

T

G

S

E

A

D

D

I

I

Q

A

K

D

E

Q

L

L

N

A

R

R

R

R

R

R

P

L

G

G

Y

Y

S

G

L

-

F

-

S

-

T

-

P

-

R

-

K

R

E

E

G

R

D

D

K

A

V

V

E

T

I

V

L

L

S

S

G

G

I

I

F

R

E

H

G

G

K

S

T

T

T

L

G

G

T

G

P

P

I

I

S

A

A

I

I

E

V

I

Y

G

N

N

T

T

N

E

Q

W

K

P

S

K

K

W

-

E

-

T

-

V

-

M

-

A

-

A

-

D

-

P

-

V

D

D

Y

P

S

A

H

E

L

L

K

A

N

D

T

V

-

A

-

R

-

N

-

A

-

P

-

L

-

T

-

R

P

P

R

R

P

P

G

G

H
|
H

A

A

D

D

L

Y

S

A

Y

G

K

M

L

L

K

K

Y

Y

G

G

N

F

Y

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

R

R

S

A

S

S

G
x
A

R

R

T

E

T

T

I

A

G

A

H

R

T

V

I

A

G

A

G

G

A

T

I

V

A

A

K

R

K

A

L

F

L

L

D

R

Y

Q

T

A

H

L

N

G

I

V

K

E

I

V

I

L

G

S

Y

H

T

V

T

I

K

S

I

I

G

G

N

A

I

-

E

-

G

-

D

-

F

-

N

-

Y

-

Y

-

N

-

S

S

I

A

E

P

N

Y

N

E

E

G

K

P

M

P

I

P

N

R

E

A

E

E

L

D

I

L

N

P

K

A

I

I

E

D

N

A

N

S

P

P

L

V

R

R

C

A

P

Y

S

D

S

K

N

A

A

A

D

E

-

A

E

D

M

M

K

I

D

A

F

Q

V

I

L

E

N

A

A

A

M

K

E

K

N

D

K

G

N

D

S

T

V

L

G

G

G

G

V

V

I

V

E

E

L

A

I

V

A

A

L

L

N

G

V

L

P

P

V

V

G

G

V

L

G

G

N

S

P

F

I

T

F

S

G

G

-

D

-

H

K

R

L

L

N

D

G

S

E

Q

L

L

S

A

N

A

A

A

I

V

M

M

N

G

I

I

N
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

I

I

G

G

R

D

G

G

F

F

E

Q

S

T

A

A

E

R

L

R

L

R

G

G

S

S

E

R

M

A

N

H

D

D

E

E

Y

M

Y

Y

Y

P

D

G

E

P

N

D

N

G

N

V

I

V

K

R

L

-

K

S

T

T

N

N

N

R

C

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

S

T

C

N

G

G

A

Q

P

P

L

L

V

R

I

V

R

R

V

A

A

A

V
x
M

K
|
K

P

P

T
x
I

P

S

S
x
T

I

V

S

P

A

R

V

A

Q

L

S

A

T

T

I

V

N

D

I

L

E

A

N

T

K

G

T

-

T

D

E

E

N

A

L

V

E

A

I

I

G

H

G

Q

R
|
R

H

S

D

D

P

V

I

C

I

A

V

V

P

P

R

A

V

A

I

G

P

V

V

V

L

V

E

E

S

T

M

M

V

V

A

A

I

L

A

V

L

L

S

A

binding flavin mononucleotide: H109 (= H104), A132 (≠ G126), Q250 (≠ N250), A251 (= A251), M308 (≠ V309), K309 (= K310), I311 (≠ T312), T313 (≠ S314), R335 (= R337)

1qxoA Crystal structure of chorismate synthase complexed with oxidized fmn and epsp (see paper)
34% identity, 94% coverage: 8:359/373 of query aligns to 1:359/388 of 1qxoA

query
sites
1qxoA

F

M

R

R

I

Y

T

L

A

T

W

A

G

G

E

E

S

S

H

H

G

G

K

P

A

R

L

L

G

T

A

A

V

I

V

I

D

E

G

G

C

I

P

P

S

A

N

G

L

L

P

P

L

L

T

T

G

A

E

E

D

D

I

I

Q

N

K

E

E

D

L

L

N

R

R

R

R
|
R

R

Q

P

G

G

G

Y

Y

S

G

L
x
R

F

G

S

G

T
x
R

P
x
M

R

K

K

I

E

E

G

N

D

D

K

Q

V

V

E

V

I

F

L

T

S

S

G

G

I

V

F

R

E

H

G

G

K

K

T

T

T

T

G

G

T

A

P

P

I

I

S

T

A

M

I

D

V

V

Y

I

N

N

T

K

N

D

-

H

Q

Q

K

K

S

W

K

L

D

D

Y

I

-

M

-

S

-

A

-

E

-

D

-

I

-

E

S

D

H

R

L

L

K

K

N

S

T

K

-

R

-

K

-

I

-

T

-

H

P

P

R

R

P

P

G

G

H
|
H

A

A

D

D

L

L

S

V

Y

G

K

G

L

I

K

K

Y

Y

G

R

N

F

Y

D

D

D

Y

L

R

R

G

N

G

S

-

L

G

E

R

R

S

S

S

S

G
x
A

R
|
R

T

E

T

T

I

T

G

M

H

R

T

V

I

A

G

V

G

G

A

A

I

V

A

A

K

K

K

R

L

L

L

L

D

-

Y

A

T

E

H

L

N

D

I

M

K

E

I

I

I

A

G

N

Y

H

T

V

T

V

K

V

I

F

G

G

N

G

I

K

E

E

G

-

D

-

F

-

N

-

Y

-

Y

-

N

-

S

-

I

I

E

D

N

V

N

P

E

E

K

N

M

L

I

T

N

V

E

A

E

E

L

I

I

K

N

Q

K

R

I

A

E

A

N

Q

N

S

P

E

L

V

R

S

C

I

P

V

S

N

S

Q

N

E

A

R

D

E

-

Q

E

E

M

I

K

K

D

D

F

Y

V

I

L

D

N

Q

A

I

M

K

E

R

N

D

K

G

N

D

S

T

V

I

G

G

G

G

V

V

I

V

E

E

L

T

I

V

A

V

L

G

N

G

V

V

P

P

V

V

G

G

V

L

G

G

N

S

P

Y

I

V

F

Q

G

W

-

D

-

R

K

K

L

L

N

D

G

A

E

R

L

L

S

A

N

Q

A

A

I

V

M

V

N

S

I

I

N
|
N

A
|
A

V

F

K

K

G

G

V

V

E

E

I

F

G

G

R

L

G

G

F

F

E

E

S

A

A

G

E

Y

L

R

L

K

G

G

S

S

E

Q

M

V

N

M

D

D

E

E

Y

I

Y

L

Y

W

D

S

E

K

N

E

N

D

N

G

I

Y

K

T

L

R

K

R

T

T

N

N

N

N

C

L

G

G

G

G

V

F

L

E

G

G

G

G

I

M

S

T

C

N

G

G

A

Q

P

P

L

I

V

V

I

V

R

R

V

G

A

V

V
x
M

K
|
K

P

P

T
x
I

P
|
P

S
x
T

I

L

S

Y

A

K

V

P

Q

L

S

M

T

S

I

V

N

D

I

I

E

E

N

T

K

H

T

E

T

P

E

Y

N

K

L

A

E

T

I

V

G

-

G

E

R

R

H

S

D

D

P

P

I

T

I

A

V

L

P

P

R

A

V

A

I

G

P

M

V

V

L

M

E

E

S

A

M

V

V

V

A

A

I

T

A

V

L

L

S

A

binding 5-[(1-carboxyvinyl)oxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: R39 (= R46), R45 (≠ L52), R48 (≠ T55), M49 (≠ P56), A133 (≠ G126), R134 (= R127)
binding flavin mononucleotide: H110 (= H104), A133 (≠ G126), N251 (= N250), A252 (= A251), M310 (≠ V309), K311 (= K310), I313 (≠ T312), P314 (= P313), T315 (≠ S314)

P0A2Y6 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
34% identity, 94% coverage: 8:359/373 of query aligns to 1:359/388 of P0A2Y6

query
sites
P0A2Y6

F

M

R

R

I

Y

T

L

A

T

W

A

G

G

E

E

S

S

H

H

G

G

K

P

A

R

L

L

G

T

A

A

V

I

V

I

D

E

G

G

C

I

P

P

S

A

N

G

L

L

P

P

L

L

T

T

G

A

E

E

D

D

I

I

Q

N

K

E

E

D

L

L

N

R

R

R

R

R

R

Q

P

G

G

G

Y

Y

S

G

L

R

F

G

S

G

T

R

P

M

R

K

K

I

E

E

G

N

D

D

K

Q

V

V

E

V

I

F

L

T

S

S

G

G

I

V

F

R

E

H

G

G

K

K

T

T

T

T

G

G

T

A

P

P

I

I

S

T

A

M

I

D

V

V

Y

I

N

N

T

K

N

D

-

H

Q

Q

K

K

S

W

K

L

D

D

Y

I

-

M

-

S

-

A

-

E

-

D

-

I

-

E

S

D

H

R

L

L

K

K

N

S

T

K

-

R

-

K

-

I

-

T

-

H

P

P

R

R

P

P

G

G

H

H

A

A

D

D

L

L

S

V

Y

G

K

G

L

I

K

K

Y

Y

G

R

N

F

Y

D

D

D

Y

L

R

R

G

N

G

S

-

L

G

E

R

R

S

S

S

S

G

A

R

R

T

E

T

T

I

T

G

M

H

R

T

V

I

A

G

V

G

G

A

A

I

V

A

A

K

K

K

R

L

L

L

L

D

-

Y

A

T

E

H

L

N

D

I

M

K

E

I

I

I

A

G

N

Y

H

T

V

T

V

K

V

I

F

G

G

N

G

I

K

E

E

G

-

D

-

F

-

N

-

Y

-

Y

-

N

-

S

-

I

I

E

D

N

V

N

P

E

E

K

N

M

L

I

T

N

V

E

A

E

E

L

I

I

K

N

Q

K

R

I

A

E

A

N

Q

N

S

P

E

L

V

R

S

C

I

P

V

S

N

S

Q

N

E

A

R

D

E

-

Q

E

E

M

I

K

K

D

D

F

Y

V

I

L

D

N

Q

A

I

M

K

E

R

N

D

K

G

N

D

S

T

V

I

G

G

G

G

V

V

I

V

E

E

L

T

I

V

A

V

L

G

N

G

V

V

P

P

V

V

G

G

V

L

G

G

N

S

P

Y

I

V

F

Q

G

W

-

D

-

R

K

K

L

L

N

D

G

A

E

R

L

L

S

A

N

Q

A

A

I

V

M

V

N

S

I

I

N
|
N

A
|
A

V

F

K

K

G

G

V

V

E

E

I

F

G

G

R

L

G

G

F

F

E

E

S

A

A

G

E

Y

L

R

L

K

G

G

S

S

E

Q

M

V

N

M

D

D

E

E

Y

I

Y

L

Y

W

D

S

E

K

N

E

N

D

N

G

I

Y

K

T

L

R

K

R

T

T

N

N

N

N

C

L

G

G

G

G

V

F

L

E

G
|
G

G

G

I

M

S

T

C

N

G

G

A

Q

P

P

L

I

V

V

I

V

R

R

V

G

A

V

V

M

K
|
K

P
|
P

T
x
I

P
|
P

S
x
T

I

L

S

Y

A

K

V

P

Q

L

S

M

T

S

I

V

N

D

I

I

E

E

N

T

K

H

T

E

T

P

E

Y

N

K

L

A

E

T

I

V

G

-

G

E

R

R

H

S

D

D

P

P

I

T

I

A

V

L

P

P

R

A

V

A

I

G

P

M

V

V

L

M

E

E

S

A

M

V

V

V

A

A

I

T

A

V

L

L

S

A

NA 251:252 (= NA 250:251) binding FMN
G296 (= G295) binding FMN
KPIPT 311:315 (≠ KPTPS 310:314) binding FMN

Query Sequence

>WP_011973405.1 NCBI__GCF_000017185.1:WP_011973405.1
MNTIGKSFRITAWGESHGKALGAVVDGCPSNLPLTGEDIQKELNRRRPGYSLFSTPRKEG
DKVEILSGIFEGKTTGTPISAIVYNTNQKSKDYSHLKNTPRPGHADLSYKLKYGNYDYRG
GGRSSGRTTIGHTIGGAIAKKLLDYTHNIKIIGYTTKIGNIEGDFNYYNSIENNEKMINE
ELINKIENNPLRCPSSNADEMKDFVLNAMENKNSVGGVIELIALNVPVGVGNPIFGKLNG
ELSNAIMNINAVKGVEIGRGFESAELLGSEMNDEYYYDENNNIKLKTNNCGGVLGGISCG
APLVIRVAVKPTPSISAVQSTINIENKTTENLEIGGRHDPIIVPRVIPVLESMVAIALSD
LMIRGGFIHPCKL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory