SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011973918.1 NCBI__GCF_000017185.1:WP_011973918.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
35% identity, 97% coverage: 1:331/340 of query aligns to 1:295/300 of 7f4cA

query
sites
7f4cA

M

M

K

K

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L

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x
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x
Y

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D

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binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ V6), G7 (= G7), Y8 (≠ F8), G9 (= G9), N10 (≠ V10), V11 (≠ I11), T37 (≠ D41), R38 (= R42), R39 (≠ S43), V72 (≠ I94), S73 (≠ T95), S74 (≠ P96), T100 (≠ A122), K102 (= K124), G127 (≠ A149), S131 (≠ G153), E185 (= E209), G280 (= G316), A284 (≠ T320)

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
35% identity, 97% coverage: 1:331/340 of query aligns to 1:297/302 of 5x9dA

query
sites
5x9dA

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active site: D196 (= D220), K200 (= K224)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ V6), G7 (= G7), Y8 (≠ F8), G9 (= G9), N10 (≠ V10), V11 (≠ I11), T37 (≠ D41), R38 (= R42), V72 (≠ I94), S73 (≠ T95), S74 (≠ P96), P82 (= P104), T100 (≠ A122), N101 (= N123), K102 (= K124), G127 (≠ A149), S131 (≠ G153), N155 (= N178), G156 (= G179), T157 (= T180), Y183 (≠ I207), A184 (= A208), E185 (= E209), D191 (= D215), D196 (= D220), K200 (= K224), A281 (= A315), G282 (= G316), A286 (≠ T320)

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
33% identity, 99% coverage: 3:340/340 of query aligns to 7:330/431 of 6dzsA

query
sites
6dzsA

I

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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G7), L12 (≠ F8), G13 (= G9), N14 (≠ V10), V15 (≠ I11), V45 (≠ D41), R46 (= R42), R47 (≠ S43), R52 (≠ N50), I63 (= I79), L78 (≠ I94), M79 (≠ T95), P84 (= P104), A102 (= A122), K104 (= K124), G306 (= G316), T310 (= T320)

Q5F8J4 NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
41% identity, 67% coverage: 105:331/340 of query aligns to 85:316/435 of Q5F8J4

query
sites
Q5F8J4

A

A

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T

D

P

S

E

R

A

D

I

I

A

K

M

Y

A

A

N

E

K

E

S

L

G

G

Y

Y

T

R

I

I

K

K

L

L

I

L

G

G

E

V

V

T

S

R

N

K

D

T

-

G

-

K

-

G

-

I

K

E

L

L

E

R

V

V

G

H

P

P

K

T

L

L

I

I

P

P

I

E

D

S

D

R

P

L

M

L

-

A

N

N

V

V

K

D

G

G

S

V

L

M

N

N

V

A

A

V

M

R

L

V

N

N

T

A

D

D

L

M

A

V

K

G

E

E

V

T

A

L

V

Y

G

G

R

A

G

G

A

A

G

G

D

A

I

L

E

P

T

T

A

A

S

S

A

A

I

V

L

V

S

A

D

D

I

I

Y

D

I

I

Sites not aligning to the query:

45 L→R: Shows a marked increase in the catalytic efficiency with NADP(+).
45:46 LS→RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; LS→RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
34% identity, 97% coverage: 1:329/340 of query aligns to 5:304/402 of 4pg7A

query
sites
4pg7A

M

L

K

N

I

I

I

A

L

L

V

L

G

G

F

L

G

G

V

T

I

V

G

G

K

S

G

G

V

V

L

V

K

K

A

I

I

I

T

E

S

E

K

N

K

R

D

Q

Y

Q

L

I

K

Q

D

D

K

T

Y

L

G

N

M

K

D

D

L

I

K

V

V

I

V

K

A

H

I

I

C

L

D

V

R

R

S

D

G

K

S

S

A

K

I

-

D

-

D

K

N

R

G

P

L

L

N

N

L

I

E

-

T

S

A

Q

L

Y

K

H

I

L

K

T

E

E

E

D

T

V

G

N

K

E

I

I

A

L

N

N

Y

-

P

-

E

D

K

D

G

S

T

L

D

D

L

I

S

-

I

I

I

V

E

E

T

V

I

M

Q

G

T

G

V

I

E

E

A

-

D

-

A

-

I

-

E

-

I

-

T

-

P

-

T

-

N

-

I

-

E

-

T

-

G

-

A

-

P

P

A

T

N

V

T

D

Y

W

M

L

L

R

E

T

A

A

F

L

K

K

N

N

G

K

K

K

H

H

V

V

I

I

T

T

A

A

N

N

K

K

G

D

P

L

L

L

A

A

V

V

N

H

F

L

K

K

E

L

L

L

I

E

D

D

C

L

A

A

N

E

E

E

N

N

S

G

K

V

L

A

F

L

R

K

F

F

E

E

A

A

S

S

V

V

G

A

G

G

A

G

M

-

P

-

I

-

N

-

L

-

A

-

N

-

D

-

A

-

L

-

A

-

G

P

N

N

E

N

I

I

F

S

E

K

I

F

K

M

G

G

I

I

L

L

N

N

G

G

T

T

T

S

N

N

Y

F

I

I

L

L

S

S

K

K

M

M

E

T

T

K

E

E

G

Q

L

T

D

T

F

F

D

E

T

E

V

A

L

L

K

D

E

E

A

A

Q

K

E

R

L

L

G

G

I

F

A

A

E

E

T

A

N

D

P

P

H

T

Q

D

D

D

I

V

S

E

G

G

L

V

D
|
D

T

A

A

A

A

R

K
|
K

I

V

V

V

I

I

L

T

A

S

N

Y

S

L

L

S

M

F

N

N

K

Q

N

V

V

I

S

K

I

L

K

N

D

D

V

V

K

K

L

R

E
x
R

G

G

I

I

T

S

R
x
G

I

V

T

T

P

L

E

T

A

D

I

I

A

N

M

V

A

A

N

D

K

Q

S

L

G

G

Y

Y

T

K

I

I

K

K

L

L

I

I

G

G

E

K

-

G

-

I

V

Y

S

E

N

N

D

G

K

K

L

V

-

N

-

A

E

S

V

V

G

E

P

P

K

T

L

L

I

I

P

D

I

K

D

K

D

H

P

Q

M

L

-

A

N

A

V

V

K

E

G

D

S

E

L

Y

N

N

V

A

A

I

M

Y

L

V

N

I

T

G

D

D

L

A

A

V

K

G

E

D

V

T

A

M

V

F

V

Y

G

G

R

K

G

G

A

A

G

G

D

S

I

L

E

A

T

T

A

G

S

S

A

A

I

V

L

V

S

S

D

D

I

L

active site: D190 (= D220), K194 (= K224)
binding lysine: R215 (≠ E245), G219 (≠ R249)

Sites not aligning to the query:

binding lysine: 394, 395

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
30% identity, 96% coverage: 3:329/340 of query aligns to 5:309/319 of 4xb1A

query
sites
4xb1A

I

I

I

S

L

I

V
x
F

G

G

F
|
F

G
|
G

V
x
T

I
x
V

G

G

K

R

G

A

V

L

L

A

K

E

A

I

I

I

T

A

S

E

K

K

K

S

D

R

Y

I

L

-

K

-

D

-

K

-

Y

F

G

G

M

V

D

E

L

L

K

N

V

V

V

I

A

S

I

I

C

T

D
|
D

R
|
R

S

S

G

G

S

T

A

I

I

W

D

G

D

D

N

-

G

-

L

F

N

D

L

L

E

L

T

E

A

A

L

K

K

E

I

V

K
|
K

E

E

E

S

T

T

G

G

K

K

I

L

A

S

N

N

Y

I

P

G

E

D

-

Y

K

E

G

V

T

Y

D

N

L

F

S

S

I

P

I

Q

E

E

T

L

I

V

Q

E

T

E

V

V

E

K

A

P

D

N

A

I

I

L

I

V

E

D

I
x
V

T
x
S

P
x
S

T

W

N

D

I

-

E

-

T

-

G

-

A

-

P

E

A

A

N

H

T

E

Y

M

M

Y

L

K

E

V

A

A

F

L

K

G

N

E

G

G

K

I

H

S

V

V

I

V

T

T

A
x
S

N

N

K
|
K

G

P

P

P

L

I

A

A

V

N

N

Y

F

Y

K

D

E

E

L

L

I

M

D

N

C

L

A

A

N

K

E

E

N

N

S

N

K

A

L

G

F

I

R

F

F

F

E

E

A
x
S

S

T

V

V

G

M

G

A

A

G

M

T

P

P

I

I

N

G

L

V

A

L

N

R

D

E

A

N

L

L

A

L

G

G

N

E

E

N

I

I

F

K

E

R

I

I

K

D

G

A

I

V

L

V

N

N

G

A

T

S

T

T

N

T

Y

F

I

I

L

L

S

T

K

K

M

M

E

-

T

S

E

E

G

G

L

K

D

T

F

L

D

D

T

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

I

I

A

L

E

E

T

E

N

D

P

P

H

S

Q

K

D

D

I

I

S

D

G

G

L

I

D
|
D

T

A

A

Y

K
|
K

I

A

V

K

I

I

L

L

A

-

N

-

S

H

L

W

M

V

N

S

K

Y

N

G

V

E

S

P

I

P

K

E

D

E

V

E

K

E

L

R

E

L

G

G

I

I

T

R

R

E

I

V

T

-

P

-

E

-

A

-

I

-

A

-

M

-

A

-

N

-

K

R

S

D

G

A

Y

R

T

N

I

V

K

R

L

L

I

V

G

A

E

Q

V

V

S

S

N

K

D

G

K

K

L

I

E

S

V

V

G

K

P

P

K

R

L

K

I

L

P

S

I

S

D

D

D

N

P

P

M

L

N

L

V

V

K

E

G

G

S

V

L

Q

N

N

V

A

A

A

M

V

L

I

N

R

T

T

D

N

L

N

A

L

K

G

E

E

V

V

A

I

V

L

V

K

G

G

R

P

G

G

A
x
G

G

G

D

G

I

R

E

V

T
|
T

A

A

S

S

A

G

I

V

L

F

S

T

D

D

I

I

active site: D211 (= D220), K215 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ V6), F10 (= F8), G11 (= G9), T12 (≠ V10), V13 (≠ I11), D39 (= D41), R40 (= R42), K57 (= K61), V91 (≠ I94), S92 (≠ T95), S93 (≠ P96), S114 (≠ A122), K116 (= K124), S141 (≠ A149), G295 (≠ A315), T300 (= T320)

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
30% identity, 96% coverage: 3:329/340 of query aligns to 5:309/319 of 4xb2A

query
sites
4xb2A

I

I

I

S

L

I

V
x
F

G

G

F
|
F

G
|
G

V
x
T

I
x
V

G

G

K

R

G

A

V

L

L

A

K

E

A

I

I

I

T

A

S

E

K

K

K

S

D

R

Y

I

L

-

K

-

D

-

K

-

Y

F

G

G

M

V

D

E

L

L

K

N

V

V

V

I

A

S

I

I

C

T

D

D

R
|
R

S

S

G

G

S

T

A

I

I

W

D

G

D

D

N

-

G

-

L

F

N

D

L

L

E

L

T

E

A

A

L

K

K

E

I

V

K

A

E

E

E

S

T

T

G

G

K

K

I

L

A

S

N

N

Y

I

P

G

E

D

-

Y

K

E

G

V

T

Y

D

N

L

F

S

S

I

P

I

Q

E

E

T

L

I

V

Q

E

T

E

V

V

E

K

A

P

D

N

A

I

I

L

I

V

E

D

I
x
V

T
x
S

P
x
S

T

W

N

D

I

-

E

-

T

-

G

-

A

-

P

E

A

A

N

H

T

E

Y

M

M

Y

L

K

E

V

A

A

F

L

K

G

N

E

G

G

K

I

H

S

V

V

I

V

T

T

A
x
S

N
|
N

K
|
K

G

P

P

P

L

I

A

A

V

N

N

Y

F

Y

K

D

E

E

L

L

I

M

D

N

C

L

A

A

N

K

E

E

N

N

S

N

K

A

L

G

F

I

R

F

F

F

E

E

A
x
S

S

T

V

V

G

M

G

A

A

G

M

T

P

P

I

I

N

G

L

V

A

L

N

R

D

E

A

N

L

L

A

L

G

G

N

E

E

N

I

I

F

K

E

R

I

I

K

D

G

A

I

V

L

V

N

N

G
x
A

T
x
S

T

T

N

T

Y

F

I

I

L

L

S

T

K

K

M

M

E

-

T

S

E

E

G

G

L

K

D

T

F

L

D

D

T

D

V

A

L

I

K

E

E

E

A

A

Q

K

E

S

L

L

G

G

I

I

A

L

E

E

T

E

N

D

P

P

H

S

Q

K

D
|
D

I

I

S

D

G

G

L

I

D
|
D

T

A

A

Y

K
|
K

I

A

V

K

I

I

L

L

A

-

N

-

S

H

L

W

M

V

N

S

K

Y

N

G

V

E

S

P

I

P

K

E

D

E

V

E

K

E

L

R

E

L

G

G

I

I

T

R

R

E

I

V

T

-

P

-

E

-

A

-

I

-

A

-

M

-

A

-

N

-

K

R

S

D

G

A

Y

R

T

N

I

V

K

R

L

L

I

V

G

A

E

Q

V

V

S

S

N

K

D

G

K

K

L

I

E

S

V

V

G

K

P

P

K

R

L

K

I

L

P

S

I

S

D

D

D

N

P

P

M

L

N

L

V

V

K

E

G

G

S

V

L

Q

N

N

V

A

A

A

M

V

L

I

N

R

T

T

D

N

L

N

A

L

K

G

E

E

V

V

A

I

V

L

V

K

G

G

R

P

G

G

A
x
G

G

G

D

G

I

R

E

V

T
|
T

A

A

S

S

A

G

I

V

L

F

S

T

D

D

I

I

active site: D211 (= D220), K215 (= K224)
binding l-homoserine: A171 (≠ G179), S172 (≠ T180), D206 (= D215), K215 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ V6), F10 (= F8), G11 (= G9), T12 (≠ V10), V13 (≠ I11), R40 (= R42), V91 (≠ I94), S92 (≠ T95), S93 (≠ P96), S114 (≠ A122), N115 (= N123), K116 (= K124), S141 (≠ A149), G295 (≠ A315), T300 (= T320)

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
32% identity, 73% coverage: 1:247/340 of query aligns to 3:236/319 of 3ingA

query
sites
3ingA

M

I

K

R

I

I

L

L

V

M

G
|
G

F
x
T

G
|
G

V
x
N

I
x
V

G

G

K

L

G

N

V

V

L

L

K

R

A

I

I

I

T

D

S

A

K

S

K

-

D

-

Y

-

L

-

K

-

D

N

K

R

Y

R

G

R

M

F

D

S

L

I

K

K

V

V

A

G

I

V

C

S

D
|
D

R
x
S

S

R

G

S

S

Y

A

A

I

S

D

G

D

R

N

N

G

-

L

L

N

D

L

I

E

S

T

S

A

I

L

I

K

S

I

N

K
|
K

E

E

E

K

T

T

G

G

K

R

I

I

A

S

N

D

Y

R

P

A

E

F

K

S

G

G

T

P

D

-

L

-

S

-

I

-

E

E

T

D

I

L

Q

M

T

G

V

E

E

A

A

A

D

D

A

L

I

L

I

V

E

D

I
x
C

T
|
T

P
|
P

T

A

N

S

I

R

E

D

T

G

G

V

A

R

P

E

A

Y

N

S

T

L

Y

Y

M

R

L

M

E

-

A

A

F

F

K

E

N

S

G

G

K

M

H

N

V

V

I

V

T

T

A
|
A

N
|
N

K
|
K

G

S

P

G

L

L

A

A

V

N

N

K

F

W

K

H

E

D

L

I

I

M

D

D

C

S

A

A

N

N

E

Q

N

N

S

S

K

K

L

Y

F

I

R

R

F

Y

E

E

A
|
A

S

T

V

V

G

A

G

G

A

G

M

V

P

P

I

L

I

F

N

S

L

V

A

L

N

D

D

Y

A

S

L

I

A

L

G

P

N

S

E

K

I

V

F

K

E

R

I

F

K

R

G

G

I

I

L

V

N

S

G

S

T

T

T

I

N

N

Y

Y

I

V

L

I

S

R

K

N

M

M

E

-

T

A

E

N

G

G

L

R

D

S

F

L

D

R

T

D

V

V

L

V

K

D

E

D

A

A

Q

I

E

K

L

K

G

G

I

I

A

A

E
|
E

T
x
S

N

N

P

P

H

Q

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

A

A

A

A

R

K
|
K

I

S

V

V

I

I

L

L

A

V

N

N

S

H

L

I

M

F

N

G

K

T

N

E

V

Y

S

T

I

L

K

N

D

D

V

V

K

E

L

Y

E

S

G

G

I

V

active site: D209 (= D220), K213 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G7), T10 (≠ F8), G11 (= G9), N12 (≠ V10), V13 (≠ I11), D38 (= D41), S39 (≠ R42), K57 (= K61), C85 (≠ I94), T86 (= T95), P87 (= P96), A112 (= A122), N113 (= N123), K114 (= K124), A139 (= A149), E198 (= E209), S199 (≠ T210)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
30% identity, 97% coverage: 1:329/340 of query aligns to 1:317/321 of 3jsaA

query
sites
3jsaA

M

M

K

K

I

T

I

I

-

N

-

L

-

S

L

I

V

F

G
|
G

F
x
L

G
|
G

V
x
N

I
x
V

G

G

K

L

G

N

V

L

L

L

K

R

A

I

I

I

T

R

S

S

K

F

K

N

D

E

Y

-

L

-

K

E

D

N

K

R

Y

L

G

G

M

L

D

K

L

F

K

N

V

V

A

F

I

V

C

A

D
|
D

R

-

S

-

G

-

S

-

A

-

I

-

D

-

N

-

G

-

L

-

N
x
S

L

L

E

H

T

S

A

Y

L

Y

K

N

I

E

K

R

E

I

E

D

T

I

G

G

K

K

I

V

A

I

N

S

Y

Y

P

K

E

E

K

K

G

G

T

S

D

L

L

D

S

S

I

L

-

E

I

Y

E

E

T

S

I

I

Q

S

T

A

V

S

E

E

A

A

-

L

-

A

-

R

-

D

-

F

D

D

A

I

I

V

E

D

I
x
A

T
|
T

P
|
P

T

A

N

S

I

A

E

D

T

-

G

G

A

K

P

K

A

E

N

L

T

A

Y

F

M

Y

L

K

E

E

A

T

F

F

K

E

N

N

G

G

K

K

H

D

V

V

I

V

T

T

A
|
A

N
|
N

K

K

G

S

P

G

L

L

A

A

V

N

N

F

F

W

K

P

E

E

L

I

I

M

D

E

C

Y

A

A

N

R

E

S

N

N

S

N

K

R

L

R

F

I

R

R

F

Y

E

E

A
|
A

S

T

V

V

G

A

G

G

A

G

M

V

P

P

I

L

I

F

N

S

L

F

A

I

N

D

D

Y

A

S

L

V

A

L

G

P

N

S

E

R

I

I

F

K

E

K

I

F

K

R

G

G

I

I

L

V

N

S

G

L

T

T

T

I

N

N

Y

Y

I

F

L

I

S

R

K

E

M

L

E

A

T

N

E

K

G

-

L

R

D

E

F

F

D

D

T

D

V

V

L

L

K

S

E

E

A

A

Q

T

E

K

L

L

G

G

I

I

A

-

E

-

T

V

N

N

P

Y

H

K

Q

D

D

D

I

L

S

T

G

G

L

L

D
|
D

T

A

A

A

A

R

K
|
K

I

S

V

V

I

I

L

L

A

C

N

N

S

H

L

L

M

Y

N

G

K

S

N

S

V

Y

S

R

I

L

K

S

D

D

V

V

K

F

L

Y

E

E

G

G

I

I

T

-

R

-

I

-

T

-

P

-

E

-

A

-

I

-

A

-

M

L

A

D

N

R

K

S

S

F

G

G

Y

K

T

N

I

E

K

R

L

L

I

V

G

T

E

E

-

T

-

G

V

I

S

V

N

N

D

G

K

K

L

P

E

S

V

A

G

E

P

S

K

R

L

I

I

K

P

S

I

L

D

D

D

S

P

N

M

D

N

Y

V

L

-

L

-

T

-

L

-

G

K

K

G

G

S

S

L

L

N

G

V

Y

A

-

M

Q

L

L

N

Q

T

T

D

D

L

T

A

N

K

G

E

T

V

L

A

N

V

V

V

S

G

D

R

L

G

Y

A

D

G

G

D

P

I

Y

E

E

T
|
T

A

A

S

G

A

A

I

V

L

M

S

N

D

D

I

L

I

V

active site: D212 (= D220), K216 (= K224)
binding nicotinamide-adenine-dinucleotide: G10 (= G7), L11 (≠ F8), G12 (= G9), N13 (≠ V10), V14 (≠ I11), D42 (= D41), S43 (≠ N53), A90 (≠ I94), T91 (= T95), P92 (= P96), A117 (= A122), N118 (= N123), A144 (= A149), T308 (= T320)

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
37% identity, 61% coverage: 81:289/340 of query aligns to 60:264/331 of 6a0sA

query
sites
6a0sA

E

E

T

P

I

F

Q

D

T

L

V

L

E

E

A

A

D

D

A

L

I

V

E

E

I
x
A

T
x
M

P

-

T

G

N

G

I

V

E

E

T

-

G

-

A

A

P

P

A

L

N

R

T

-

Y

L

M

V

L

L

E

P

A

A

F

L

K

E

N

A

G

G

K

I

H

P

V

L

I

I

T

T

A
|
A

N

N

K
|
K

G

A

P

L

L

L

A

A

V

E

N

A

F

W

K

E

E

S

L

L

I

R

D

P

C

F

A

A

N

E

E

E

N

G

S

-

K

-

L

L

F

I

R

Y

F

H

E

E

A

A

S

S

V

V

G

M

G

A

A

G

M

T

P

P

I

A

I

L

N

S

L

F

A

L

N

-

D

E

A

T

L

L

A

R

G

G

N

S

E

E

I

L

F

L

E

E

I

L

K

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

T

T

N

L

Y

Y

I

I

L

L

S

Q

K

E

M

M

E

E

T

-

E

K

G

G

L

R

D

T

F

Y

D

A

T

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

I
x
Y

A

A

E
|
E

T

A

N

D

P

P

H

T

Q

L

D
|
D

I

V

S

E

G

G

L

I

D
|
D

T

A

A

A

A

H

K
|
K

I

L

V

T

I

L

L

L

A

A

N

R

S

L

L

L

M

V

N

D

K

P

N

G

V

F

S

P

I

F

K

A

D

E

V

V

K

E

L

A

E

Q

G

G

I

I

T

A

R

R

I

L

T

T

P

P

E

E

A

V

I

L

A

Q

M

K

A

A

N

E

K

A

S

R

G

G

Y

E

T

R

I

V

K

R

L

L

I

V

-

A

-

S

-

L

-

F

G

G

E

E

V

G

S

G

N

R

D

W

K

R

L

A

E

A

V

V

G

A

P

P

K

R

L

R

I

L

P

P

I

Q

D

D

D

H

P

P

M

L

active site: D191 (= D220), K195 (= K224)
binding l-homoserine: K99 (= K124), N150 (= N178), G151 (= G179), T152 (= T180), Y178 (≠ I207), E180 (= E209), D186 (= D215), K195 (= K224)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: A73 (≠ I94), M74 (≠ T95), A97 (= A122), K99 (= K124), G177 (= G206), E180 (= E209)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 11, 12, 13, 14, 42, 43, 44, 45, 48, 50, 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
37% identity, 61% coverage: 81:289/340 of query aligns to 60:264/331 of 2ejwA

query
sites
2ejwA

E

E

T

P

I

F

Q

D

T

L

V

L

E

E

A

A

D

D

A

L

I

V

E

E

I

A

T

M

P

-

T

G

N

G

I

V

E

E

T

-

G

-

A

A

P

P

A

L

N

R

T

-

Y

L

M

V

L

L

E

P

A

A

F

L

K

E

N

A

G

G

K

I

H

P

V

L

I

I

T

T

A

A

N

N

K

K

G

A

P

L

L

L

A

A

V

E

N

A

F

W

K

E

E

S

L

L

I

R

D

P

C

F

A

A

N

E

E

E

N

G

S

-

K

-

L

L

F

I

R

Y

F

H

E
|
E

A

A

S

S

V
|
V

G
x
M

G
x
A

A

G

M
x
T

P

P

I

A

I

L

N

S

L

F

A

L

N

-

D

E

A

T

L

L

A

R

G

G

N

S

E

E

I

L

F

L

E

E

I

L

K

H

G

G

I

I

L

L

N

N

G

G

T

T

N

L

Y

Y

I

I

L

L

S

Q

K

E

M

M

E

E

T

-

E

K

G

G

L

R

D

T

F

Y

D

A

T

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G

G

I

Y

A

A

E

E

T

A

N

D

P

P

H

T

Q

L

D

D

I

V

S

E

G

G

L

I

D
|
D

T

A

A

A

A

H

K
|
K

I

L

V

T

I

L

L

L

A

A

N

R

S

L

L

L

M

V

N

D

K

P

N

G

V

F

S

P

I

F

K

A

D

E

V

V

K

E

L

A

E

Q

G

G

I

I

T

A

R

R

I

L

T

T

P

P

E

E

A

V

I

L

A

Q

M

K

A

A

N

E

K

A

S

R

G

G

Y

E

T

R

I

V

K

R

L

L

I

V

-

A

-

S

-

L

-

F

G

G

E

E

V

G

S

G

N

R

D

W

K

R

L

A

E

A

V

V

G

A

P

P

K

R

L

R

I

L

P

P

I

Q

D

D

D

H

P

P

M

L

active site: D191 (= D220), K195 (= K224)
binding magnesium ion: E121 (= E148), V124 (= V151), M125 (≠ G152), A126 (≠ G153), T128 (≠ M155)

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
37% identity, 61% coverage: 81:289/340 of query aligns to 60:264/332 of 6a0tB

query
sites
6a0tB

E

E

T

P

I

F

Q

D

T

L

V

L

E

E

A

A

D

D

A

L

I

V

E

E

I
x
A

T
x
M

P

-

T
x
G

N

G

I

V

E

E

T

-

G

-

A

A

P

P

A

L

N

R

T

-

Y

L

M

V

L

L

E

P

A

A

F

L

K

E

N

A

G

G

K

I

H

P

V

L

I

I

T

T

A
|
A

N
|
N

K

K

G

A

P

L

L

L

A

A

V

E

N

A

F

W

K

E

E

S

L

L

I

R

D

P

C

F

A

A

N

E

E

E

N

G

S

-

K

-

L

L

F

I

R

Y

F

H

E

E

A

A

S

S

V

V

G

M

G

A

A

G

M

T

P

P

I

A

I

L

N

S

L

F

A

L

N

-

D

E

A

T

L

L

A

R

G

G

N

S

E

E

I

L

F

L

E

E

I

L

K

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

T

T

N

L

Y

Y

I

I

L

L

S

Q

K

E

M

M

E

E

T

-

E

K

G

G

L

R

D

T

F

Y

D

A

T

E

V

A

L

L

K

L

E

E

A

A

Q

Q

E

R

L

L

G
|
G

I
x
Y

A

A

E
|
E

T

A

N

D

P

P

H

T

Q

L

D
|
D

I

V

S

E

G

G

L

I

D
|
D

T

A

A

A

A

H

K
|
K

I

L

V

T

I

L

L

L

A

A

N

R

S

L

L

L

M

V

N

D

K

P

N

G

V

F

S

P

I

F

K

A

D

E

V

V

K

E

L

A

E

Q

G

G

I

I

T

A

R

R

I

L

T

T

P

P

E

E

A

V

I

L

A

Q

M

K

A

A

N

E

K

A

S

R

G

G

Y

E

T

R

I

V

K

R

L

L

I

V

-

A

-

S

-

L

-

F

G

G

E

E

V

G

S

G

N

R

D

W

K

R

L

A

E

A

V

V

G

A

P

P

K

R

L

R

I

L

P

P

I

Q

D

D

D

H

P

P

M

L

active site: D191 (= D220), K195 (= K224)
binding l-homoserine: N150 (= N178), G151 (= G179), T152 (= T180), Y178 (≠ I207), E180 (= E209), D186 (= D215), K195 (= K224)
binding nadp nicotinamide-adenine-dinucleotide phosphate: A73 (≠ I94), M74 (≠ T95), G75 (≠ T97), A97 (= A122), N98 (= N123), G177 (= G206), E180 (= E209)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 10, 11, 12, 13, 14, 42, 43, 44, 45, 48, 50, 289, 290, 294

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
27% identity, 96% coverage: 5:331/340 of query aligns to 562:908/916 of O81852

query
sites
O81852

L

I

V

V

G

G

F

P

G

G

V

L

I

I

G

G

K

A

G

T

V

L

L

L

K

D

A

Q

I

L

T

R

S

D

K

Q

K

A

D

A

Y

V

L

L

K

K

D

Q

K

E

Y

F

G

N

M

I

D

D

L

L

K

R

V

V

V

L

A

G

I

I

C

T

D

G

R

S

S

K

G

K

S

M

A

L

I

L

D

S

D

D

N

I

G

G

L

I

N

D

L

L

E

S

T

R

A

W

L

R

K

E

I

L

K

L

E

N

E

E

T

-

G

-

K

-

I

-

A

-

N

-

Y

-

P

-

E

-

K

K

G

G

T

T

D

E

L

A

S

D

I

L

I

D

E

K

T

F

I

T

Q

Q

T

Q

V

V

E

H

A

G

D

N

A

H

I

F

I

I

E

-

I

-

T

-

P

P

T

N

N

S

I

V

E

V

T

V

G

D

A

C

P

T

A

A

N

D

T

S

Y

A

M

I

L

A

E

S

A

R

F

Y

-

Y

-

D

-

W

-

L

K

R

N

K

G

G

K

I

H

H

V

V

I

I

T

T

A

P

N

N

K

K

-

K

-

A

-

N

-

S

G

G

P

P

L

L

A

D

V

-

N

Q

F

Y

K

L

E

K

L

L

I

R

D

D

C

L

A

Q

N

R

E

K

N

S

S

Y

K

T

L

H

F

Y

R

F

F

Y

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

T

A

L

N

R

D

G

A

L

L

L

-

E

A

T

G

G

N

D

E

K

I

I

F

L

E

R

I

I

K

E

G

G

I

I

L

C

N

S

G

G

T

T

T

L

N

S

Y

Y

I

L

L

F

S

N

K

N

M

F

E

V

T

G

E

D

G

-

L

R

D

S

F

F

D

S

T

E

V

V

L

V

K

T

E

E

A

A

Q

K

E

N

L

A

G

G

I

F

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

S

G

G

L

T

D

D

T

V

A

A

A

R

K

K

I

V

V

I

I

I

L

L

A

A

N

R

S

E

-

S

-

G

-

L

-

K

L

L

M

D

N

L

K

A

N

D

V

L

S

P

I

I

K

R

D

S

V

L

-

V

-

P

-

E

K

P

L

L

E

K

G

G

I

C

T

T

R

S

I

V

T

E

P

E

-

F

-

M

E

E

A

K

I

L

A

P

M

Q

-

Y

-

D

-

G

-

D

-

L

-

A

-

K

-

E

-

R

-

L

-

D

A

A

N

E

K

N

S

S

G

G

Y

E

T

V

I

L

K

R

L

Y

I

V

G

G

E

V

V

V

S

D

-

A

-

V

N

N

D

Q

K

K

L

G

E

T

V

V

G

E

P

L

K

R

L

R

I

Y

P

K

I

K

D

E

D

H

P

P

M

F

-

A

N

Q

V

L

K

A

G

G

S

S

L

D

N

N

V

I

A

I

M

A

L

F

N

T

T

T

D

T

L

R

A

Y

K

K

E

D

-

H

-

P

V

L

A

I

V

V

V

R

G

G

R

P

G

G

A

A

G

G

D

A

I

Q

E

V

T

T

A

A

S

G

A

G

I

I

L

F

S

S

D

D

I

I

I

L

Y

R

I

L

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

O94671 Probable homoserine dehydrogenase; HDH; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 45% coverage: 118:270/340 of query aligns to 117:298/376 of O94671

query
sites
O94671

H

N

V

I

I

A

T

T

A

P

N

N

K

K

G

K

P

A

L

F

A

S

V

A

N

S

-

N

-

D

-

V

F

Y

K

Q

E

N

L

I

I

I

D

K

C

A

A

S

N

K

E

E

N

S

S

G

K

A

L

L

F

L

R

M

F

H

E

E

A

A

S

S

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

T

A

L

N

K

D

E

A

L

L

I

A

A

-

T

G

G

N

D

E

E

I

I

F

I

E

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

S

N

K

V

M

W

E

S

T

P

E

N

G

G

-

K

-

G

-

T

L

A

D
x
S

F

F

D

S

T

D

V

I

L

V

K

K

E

I

A

A

Q

K

E

Q

L

N

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

M

D

D

T

V

A

A

A

R

K

K

I

V

V

T

I

I

L

L

-

S

-

R

-

I

-

A

-

G

-

V

-

H

-

V

-

E

-

S

A

A

N

S

S

S

L

F

M

P

N

V

K

K

N

S

V

L

S

I

I

P

K

E

D

P

V

L

K

K

-

S

-

A

-

V

-

N

-

A

-

E

-

F

L

L

E

A

G

G

I

L

T

P

R

N

I

F

T

D

P

S

E

E

A

F

I

A

A

S

M

M

-

R

-

E

A

A

N

E

K

K

S

E

G

G

Y

K

T

V

I

V

K

R

L

F

I

V

G

G

E

E

S201 (≠ D194) modified: Phosphoserine

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
33% identity, 34% coverage: 114:228/340 of query aligns to 106:226/358 of 1tveA

query
sites
1tveA

K

E

N

N

G

G

K

I

H

S

V

I

I

A

T

T

A

P

N

N

K
|
K

G

K

P

A

L

F

A

S

-

S

-

D

-

L

V

A

N

T

F

W

K

K

E

A

L

L

I

F

D

S

C

N

A

K

N

P

E

T

N

N

S

G

K

F

L

V

F

Y

R

H

F

-

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

N

R

D

E

A

I

L

I

-

Q

A

T

G

G

N

D

E

E

I

V

F

E

E

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T
|
T

T

L

N

S

Y

Y

I

I

L

F

S

N

K

E

M

F

E

S

T

T

E

S

G

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D
|
D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

active site: D218 (= D220), K222 (= K224)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K124), T175 (= T180), D213 (= D215), D218 (= D220)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
33% identity, 34% coverage: 114:228/340 of query aligns to 106:226/358 of 1q7gA

query
sites
1q7gA

K

E

N

N

G

G

K

I

H

S

V

I

I

A

T

T

A
x
P

N

N

K
|
K

G

K

P

A

L

F

A

S

-

S

-

D

-

L

V

A

N

T

F

W

K

K

E

A

L

L

I

F

D

S

C

N

A

K

N

P

E

T

N

N

S

G

K

F

L

V

F

Y

R

H

F

-

E

E

A
|
A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

N

R

D

E

A

I

L

I

-

Q

A

T

G

G

N

D

E

E

I

V

F

E

E

K

I

I

K

E

G

G

I

I

L

F

N
x
S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

S

N

K

E

M

F

E

S

T

T

E

S

G

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

active site: D218 (= D220), K222 (= K224)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A122), K116 (= K124), A143 (= A149), S173 (≠ N178), K222 (= K224)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
33% identity, 34% coverage: 114:228/340 of query aligns to 106:226/358 of 1ebuD

query
sites
1ebuD

K

E

N

N

G

G

K

I

H

S

V

I

I

A

T

T

A

P

N

N

K
|
K

G

K

P

A

L

F

A

S

-

S

-

D

-

L

V

A

N

T

F

W

K

K

E

A

L

L

I

F

D

S

C

N

A

K

N

P

E

T

N

N

S

G

K

F

L

V

F

Y

R

H

F

-

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

N

R

D

E

A

I

L

I

-

Q

A

T

G

G

N

D

E

E

I

V

F

E

E

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

S

N

K

E

M

F

E

S

T

T

E

S

G

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

active site: D218 (= D220), K222 (= K224)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K124)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
33% identity, 34% coverage: 114:228/340 of query aligns to 106:226/358 of 1ebfA

query
sites
1ebfA

K

E

N

N

G

G

K

I

H

S

V

I

I

A

T

T

A
x
P

N

N

K

K

G

K

P

A

L

F

A

S

-

S

-

D

-

L

V

A

N

T

F

W

K

K

E

A

L

L

I

F

D

S

C

N

A

K

N

P

E

T

N

N

S

G

K

F

L

V

F

Y

R

H

F

-

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

N

R

D

E

A

I

L

I

-

Q

A

T

G

G

N

D

E

E

I

V

F

E

E

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

S

N

K

E

M

F

E

S

T

T

E

S

G

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E

E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

active site: D218 (= D220), K222 (= K224)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A122)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

P31116 Homoserine dehydrogenase; HDH; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
33% identity, 34% coverage: 114:228/340 of query aligns to 107:227/359 of P31116

query
sites
P31116

K

E

N

N

G

G

K

I

H

S

V

I

I

A

T

T

A

P

N

N

K
|
K

G

K

P

A

L

F

A

S

-

S

-

D

-

L

V

A

N

T

F

W

K

K

E

A

L

L

I

F

D

S

C

N

A

K

N

P

E

T

N

N

S

G

K

F

L

V

F

Y

R

H

F

-

E

E

A

A

S

T

V

V

G

G

G

A

A

G

M

L

P

P

I

I

N

S

L

F

A

L

N

R

D

E

A

I

L

I

-

Q

A

T

G

G

N

D

E

E

I

V

F

E

E

K

I

I

K

E

G

G

I

I

L

F

N

S

G

G

T

T

T

L

N

S

Y

Y

I

I

L

F

S

N

K

E

M

F

E

S

T

T

E

S

G

Q

-

A

-

N

-

D

L

V

D

K

F

F

D

S

T

D

V

V

L

V

K

K

E

V

A

A

Q

K

E

K

L

L

G

G

I

Y

A

T

E
|
E

T

P

N

D

P

P

H

R

Q

D

D

D

I

L

S

N

G

G

L

L

D
|
D

T

V

A

A

A

R

K
|
K

I

V

V

T

I

I

L

V

K117 (= K124) binding
E208 (= E209) binding ; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D220) mutation to L: Reduces kcat 150-fold.
K223 (= K224) mutation to V: Loss of activity.

Sites not aligning to the query:

11:18 binding
93 binding

P80053 Glutamate dehydrogenase 2; GDH-2; EC 1.4.1.3 from Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus) (see paper)
32% identity, 21% coverage: 2:72/340 of query aligns to 216:276/420 of P80053

query
sites
P80053

K

R

I

V

I

I

L

I

V

Q

G

G

F

F

G

G

V

N

I

V

G

G

K

Y

G

Y

V

A

L

G

K

K

A

F

I

L

T

S

S

E

K

-

D

-

Y

-

L

-

K

-

D

-

K

-

Y

-

G

-

M

M

D

G

L

A

K

K

V

I

V

V

A

G

I

V

C

S

D

D

R

S

S

K

G

G

S

G

A

V

I

I

D

N

D

E

N
x
K

G

G

L

I

N

D

L

V

E

G

T
x
K

A

A

L

I

K

E

I

I

K

K

E

E

E

K

T

T

G

G

K

S

I

V

A

I

N

N

Y

Y

P

P

E

E

K254 (≠ N50) modified: N6-methyllysine
K260 (≠ T56) modified: N6-methyllysine

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylmethionine
372 modified: N6-methyllysine
391 modified: N6-methyllysine
392 modified: N6-methyllysine

Query Sequence

>WP_011973918.1 NCBI__GCF_000017185.1:WP_011973918.1
MKIILVGFGVIGKGVLKAITSKKDYLKDKYGMDLKVVAICDRSGSAIDDNGLNLETALKI
KEETGKIANYPEKGTDLSIIETIQTVEADAIIEITPTNIETGAPANTYMLEAFKNGKHVI
TANKGPLAVNFKELIDCANENSKLFRFEASVGGAMPIINLANDALAGNEIFEIKGILNGT
TNYILSKMETEGLDFDTVLKEAQELGIAETNPHQDISGLDTAAKIVILANSLMNKNVSIK
DVKLEGITRITPEAIAMANKSGYTIKLIGEVSNDKLEVGPKLIPIDDPMNVKGSLNVAML
NTDLAKEVAVVGRGAGDIETASAILSDIIYIYENNIKWNR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory