SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011974345.1 NCBI__GCF_000017145.1:WP_011974345.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WGB5 O-succinylhomoserine sulfhydrylase; OSH sulfhydrylase; OSHS sulfhydrylase; EC 2.5.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
52% identity, 97% coverage: 8:389/394 of query aligns to 20:405/406 of P9WGB5

query
sites
P9WGB5

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K219 (= K207) modified: N6-(pyridoxal phosphate)lysine

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
42% identity, 98% coverage: 9:393/394 of query aligns to 10:396/396 of 4omaA

query
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active site: R59 (= R57), Y112 (≠ F110), D184 (= D182), K209 (= K207)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G85), I88 (≠ M86), Y112 (≠ F110), D184 (= D182), S206 (= S204), T208 (= T206), K209 (= K207), V337 (≠ N334), S338 (≠ N335), R373 (= R370)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
42% identity, 98% coverage: 9:393/394 of query aligns to 10:396/396 of 3jwbA

query
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3jwbA

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active site: R59 (= R57), Y112 (≠ F110), D184 (= D182), K209 (= K207)
binding norleucine: Y112 (≠ F110), K209 (= K207), S338 (≠ N335), R373 (= R370)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
42% identity, 98% coverage: 9:393/394 of query aligns to 10:396/396 of 3jwaA

query
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3jwaA

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R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

V

F

V

L

E

S

T

H

L

S

A

M

P

P

K

K

Y

F

G

G

V

I

E

N

C

V

T

S

L

F

V

V

D

D

G

A

R

A

D

K

L

P

K

E

N

E

W

I

E

R

D

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

F

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

G

T

V

V

A

A

R

G

L

I

A

A

D

H

Q

Q

I

Q

G

G

A

A

K

L

V

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S

S

A

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

L

V

S

G

D

K

K

Q

Q

E

W

F

I

I

D

D

E

Q

-

A

-

R

-

F

-

V

N

G

L

L

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

G

A

C

M

M

S

S

P

P

F

F

N

N

A

A

W

W

T

L

L

T

L

L

K

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

R

L

R

K

I

I

A

A

D

R

F

F

L

L

T

E

E

G

Q

H

P

P

Q

S

V

I

A

T

R

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

K

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

K

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

V

I

A

S

F

F

E

E

L

I

K

A

G

G

K

L

E

E

A

A

A

G

F

R

A

R

L

M

Q

I

N

N

A

S

L

V

E

E

I

L

V

C

R

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

E

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

A

D

G

G

T

L

V

I

R
|
R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

P

E

E

D

D

L

I

V

I

E

N

D

D

F

L

A

E

K

H

A

A

L

I

R

R

S

K

V

A

T

T

active site: R59 (= R57), Y112 (≠ F110), D184 (= D182), K209 (= K207)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (≠ F110), K209 (= K207), V337 (≠ N334), S338 (≠ N335), R373 (= R370)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
42% identity, 98% coverage: 9:393/394 of query aligns to 10:396/396 of 3jw9A

query
sites
3jw9A

T

T

Q

Q

L

I

V

V

H

H

G

A

G

G

T

Q

L

Q

R

P

S

D

-

P

Q

S

Y

T

G

G

E

A

T

L

S

S

E

T

A

P

I

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

T

S

S

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

T

E

D

S

G

G

Y

Y

I

I

Y

Y

A

T

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

M

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G

G

M

I

A

S

A

A

V

I

S

T

A

T

I

L

L

L

C

T

Q

L

V

C

K

Q

A

Q

G

G

D

D

H

H

I

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

V

F

V

L

E

S

T

H

L

S

A

M

P

P

K

K

Y

F

G

G

V

I

E

N

C

V

T

S

L

F

V

V

D

D

G

A

R

A

D

K

L

P

K

E

N

E

W

I

E

R

D

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

F

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

G

T

V

V

A

A

R

G

L

I

A

A

D

H

Q

Q

I

Q

G

G

A

A

K

L

V

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S

S

A

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

L

V

S

G

D

K

K

Q

Q

E

W

F

I

I

D

D

E

Q

-

A

-

R

-

F

-

V

N

G

L

L

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

G

A

C

M

M

S

S

P

P

F

F

N

N

A

A

W

W

T

L

L

T

L

L

K

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

R

L

R

K

I

I

A

A

D

R

F

F

L

L

T

E

E

G

Q

H

P

P

Q

S

V

I

A

T

R

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

K

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

K

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

V

I

A

S

F

F

E

E

L

I

K

A

G

G

K

L

E

E

A

A

A

G

F

R

A

R

L

M

Q

I

N

N

A

S

L

V

E

E

I

L

V

C

R

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

E

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

A

D

G

G

T

L

V

I

R

R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

P

E

E

D

D

L

I

V

I

E

N

D

D

F

L

A

E

K

H

A

A

L

I

R

R

S

K

V

A

T

T

active site: R59 (= R57), Y112 (≠ F110), D184 (= D182), K209 (= K207)
binding S-ethyl-L-cysteine: Y112 (≠ F110), K209 (= K207), V337 (≠ N334), S338 (≠ N335)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
42% identity, 98% coverage: 9:393/394 of query aligns to 9:395/395 of 5m3zA

query
sites
5m3zA

T

T

Q

Q

L

I

V

V

H

H

G

A

G

G

T

Q

L

Q

R

P

S

D

-

P

Q

S

Y

T

G

G

E

A

T

L

S

S

E

T

A

P

I

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

T

S

S

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

T

E

D

S

G

G

Y

Y

I

I

Y

Y

A

T

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

M

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

S

T

A

T

I

L

L

L

C

T

Q

L

V

C

K

Q

A

Q

G

G

D

D

H

H

I

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S
x
H

C

T

R

H

W

A

V

F

V

L

E

S

T

H

L

S

A

M

P

P

K

K

Y

F

G

G

V

I

E

N

C

V

T

S

L

F

V

V

D

D

G

A

R

A

D

K

L

P

K

E

N

E

W

I

E

R

D

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

F

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

G

T

V

V

A

A

R

G

L

I

A

A

D

H

Q

Q

I

Q

G

G

A

A

K

L

V

L

V

V

D
|
D

N

N

V
x
T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

A

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

L

V

S

G

D

K

K

Q

Q

E

W

F

I

I

D

D

E

Q

-

A

-

R

-

F

-

V

N

G

L

L

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

G

A

C

M

M

S

S

P

P

F

F

N

N

A

A

W

W

T

L

L

T

L

L

K

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

R

L

R

K

I

I

A

A

D

R

F

F

L

L

T

E

E

G

Q

H

P

P

Q

S

V

I

A

T

R

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

K

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

K

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

V

I

A

S

F

F

E

E

L

I

K

A

G

G

K

L

E

E

A

A

A

G

F

R

A

R

L

M

Q

I

N

N

A

S

L

V

E

E

I

L

V

C

R

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

E

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

A

D

G

G

T

L

V

I

R
|
R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

P

E

E

D

D

L

I

V

I

E

N

D

D

F

L

A

E

K

H

A

A

L

I

R

R

S

K

V

A

T

T

active site: R58 (= R57), Y111 (≠ F110), D183 (= D182), K208 (= K207)
binding norleucine: Y111 (≠ F110), H113 (≠ S112), K208 (= K207), V336 (≠ N334), S337 (≠ N335)
binding pyridoxal-5'-phosphate: G86 (= G85), I87 (≠ M86), Y111 (≠ F110), E154 (= E153), D183 (= D182), T185 (≠ V184), S205 (= S204), T207 (= T206), K208 (= K207)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G85), I87 (≠ M86), Y111 (≠ F110), D183 (= D182), S205 (= S204), T207 (= T206), K208 (= K207), V336 (≠ N334), S337 (≠ N335), R372 (= R370)

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
42% identity, 98% coverage: 9:393/394 of query aligns to 10:396/396 of 6egrA

query
sites
6egrA

T

T

Q

Q

L

I

V

V

H

H

G

A

G

G

T

Q

L

Q

R

P

S

D

-

P

Q

S

Y

T

G

G

E

A

T

L

S

S

E

T

A

P

I

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

T

S

S

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

T

E

D

S

G

G

Y

Y

I

I

Y

Y

A

T

R

R

Y

L

G

G

S

N

P

P

T

T

N

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

M

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

S

T

A

T

I

L

L

L

C

T

Q

L

V

C

K

Q

A

Q

G

G

D

D

H

H

I

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

V

F

V

L

E

S

T

H

L

S

A

M

P

P

K

K

Y

F

G

G

V

I

E

N

C

V

T

S

L

F

V

V

D

D

G

A

R

A

D

K

L

P

K

E

N

E

W

I

E

R

D

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

F

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

G

T

V

V

A

A

R

G

L

I

A

A

D

H

Q

Q

I

Q

G

G

A

A

K

L

V

L

V

V

D
|
D

N

N

V
x
T

F
|
F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

A

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

L

V

S

G

D

K

K

Q

Q

E

W

F

I

I

D

D

E

Q

-

A

-

R

-

F

-

V

N

G

L

L

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

G

A

C

M

M

S

S

P

P

F

F

N

N

A

A

W

W

T

L

L

T

L

L

K

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

R

L

R

K

I

I

A

A

D

R

F

F

L

L

T

E

E

G

Q

H

P

P

Q

S

V

I

A

T

R

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

K

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

K

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

V

I

A

S

F

F

E

E

L

I

K

A

G

G

K

L

E

E

A

A

A

G

F

R

A

R

L

M

Q

I

N

N

A

S

L

V

E

E

I

L

V

C

R

L

I

L

S

A

N

V

N

S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

P

L

Y

T

A

D

P

E

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

A

D

G

G

T

L

V

I

R

R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

P

E

E

D

D

L

I

V

I

E

N

D

D

F

L

A

E

K

H

A

A

L

I

R

R

S

K

V

A

T

T

binding pyridoxal-5'-phosphate: G87 (= G85), I88 (≠ M86), Y112 (≠ F110), E155 (= E153), D184 (= D182), T186 (≠ V184), F187 (= F185), S206 (= S204), T208 (= T206), K209 (= K207)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
42% identity, 98% coverage: 9:393/394 of query aligns to 10:396/396 of 4hf8A

query
sites
4hf8A

T

T

Q

Q

L

I

V

V

H

H

G

A

G

G

T

Q

L

Q

R

P

S

D

-

P

Q

S

Y

T

G

G

E

A

T

L

S

S

E

T

A

P

I

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

T

S

S

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

T

E

D

S

G

G

Y

Y

I

I

Y

Y

A

T

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

M

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

S

T

A

T

I

L

L

L

C

T

Q

L

V

C

K

Q

A

Q

G

G

D

D

H

H

I

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

V

F

V

L

E

S

T

H

L

S

A

M

P

P

K

K

Y

F

G

G

V

I

E

N

C

V

T

R

L

F

V

V

D

D

G

A

R

A

D

K

L

P

K

E

N

E

W

I

E

R

D

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

F

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N
|
N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

G

T

V

V

A

A

R

G

L

I

A

A

D

H

Q

Q

I

Q

G

G

A

A

K

L

V

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

A

V

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

L

V

S

G

D

K

K

Q

Q

E

W

F

I

I

D

D

E

Q

-

A

-

R

-

F

-

V

N

G

L

L

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

G

A

C

M

M

S

S

P

P

F

F

N

N

A

A

W

W

T

L

L

T

L

L

K

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

R

L

R

K

I

I

A

A

D

R

F

F

L

L

T

E

E

G

Q

H

P

P

Q

S

V

I

A

T

R

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

K

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

K

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

V

I

A

S

F

F

E

E

L

I

K

A

G

G

K

L

E

E

A

A

A

G

F

R

A

R

L

M

Q

I

N

N

A

S

L

V

E

E

I

L

V

C

R

L

I

L

S

A

N

V

N
x
S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

E

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

A

D

G

G

T

L

V

I

R
|
R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

P

E

E

D

D

L

I

V

I

E

N

D

D

F

L

A

E

K

H

A

A

L

I

R

R

S

K

V

A

T

T

active site: R59 (= R57), Y112 (≠ F110), D184 (= D182), K209 (= K207)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G85), I88 (≠ M86), Y112 (≠ F110), E155 (= E153), N159 (= N157), D184 (= D182), S206 (= S204), K209 (= K207), S338 (≠ N335), R373 (= R370)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
41% identity, 98% coverage: 9:393/394 of query aligns to 10:385/386 of 3mkjA

query
sites
3mkjA

T

T

Q

Q

L

I

V

V

H

H

G

A

G

G

T

Q

L

Q

R

P

S

D

-

P

Q

S

Y

T

G

G

E

A

T

L

S

S

E

T

A

P

I

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

T

S

S

A

E

E

A

Q

A

G

E

A

A

A

R

R

F

F

K

-

G

-

E

-

T

-

D

-

G

-

Y

-

I

-

Y

-

A

-

R

-

Y

L

G

G

S

N

P

P

T

T

N

T

D

D

M

A

F

L

E

E

K

K

R

K

M

L

C

A

M

V

L

L

E

E

G

R

A

G

E

E

D

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

S

T

A

T

I

L

L

L

C

T

Q

L

V

C

K

Q

A

Q

G

G

D

D

H

H

I

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

V

F

V

L

E

S

T

H

L

S

A

M

P

P

K

K

Y

F

G

G

V

I

E

N

C

V

T

S

L

F

V

V

D

D

G

A

R

A

D

K

L

P

K

E

N

E

W

I

E

R

D

A

A

A

V

M

R

R

P

P

N

E

T

T

K

K

V

V

F

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

V

L

I

V

D

D

I

I

A

E

G

T

V

V

A

A

R

G

L

I

A

A

D

H

Q

Q

I

Q

G

G

A

A

K

L

V

L

V

V

D
|
D

N

N

V

T

F
|
F

A

M

T

S

P

P

L

Y

F

C

Q

Q

K

Q

P

P

L

L

E

Q

L

L

G

G

A

A

H

D

I

I

V

V

Y

H

S
|
S

A

V

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

L

V

S

G

D

K

K

Q

Q

E

W

F

I

I

D

D

E

Q

-

A

-

R

-

F

-

V

N

G

L

L

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

G

A

C

M

M

S

S

P

P

F

F

N

N

A

A

W

W

T

L

L

T

L

L

K

R

G

G

I

V

E

K

T

T

L

L

P

G

L

I

R

R

V

M

R

E

Q

R

Q

H

T

C

E

E

S

N

A

A

R

L

R

K

I

I

A

A

D

R

F

F

L

L

T

E

E

G

Q

H

P

P

Q

S

V

I

A

T

R

R

V

V

I

Y

Y

Y

P

P

G

G

R

L

K

S

D

S

H

H

P

P

Q

Q

A

Y

D

E

I

L

I

G

A

Q

K

R

Q

Q

M

M

S

S

G

L

G

P

S

G

T

G

L

I

V

I

A

S

F

F

E

E

L

I

K

A

G

G

K

L

E

E

A

A

A

G

F

R

A

R

L

M

Q

I

N

N

A

S

L

V

E

E

I

L

V

C

R

L

I

L

S

A

N

V

N

S

L

L

G

G

D

D

S

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

P

L

V

T

A

D

P

E

E

A

E

R

R

A

L

E

K

L

A

G

G

I

I

S

T

A

D

G

G

T

L

V

I

R

R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

P

E

E

D

D

L

I

V

I

E

N

D

D

F

L

A

E

K

H

A

A

L

I

R

R

S

K

V

A

T

T

active site: Y101 (≠ F110), D173 (= D182), K198 (= K207)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G85), I77 (≠ M86), Y101 (≠ F110), E144 (= E153), D173 (= D182), F176 (= F185), S195 (= S204), T197 (= T206), K198 (= K207)

5dx5A Crystal structure of methionine gamma-lyase from clostridium sporogenes (see paper)
39% identity, 97% coverage: 8:389/394 of query aligns to 9:394/399 of 5dx5A

query
sites
5dx5A

A

A

T

T

Q

K

L

A

V

I

H

H

G

G

T

H

L

I

-

G

R

D

S

K

Q

Q

Y

F

G

G

E

S

T

L

S

A

E

T

A

P

I

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

I

Y

F

D

D

T

S

S

A

E

E

A

Q

A

G

E

G

A

R

R

R

F

F

K

A

G

G

E

E

T

E

D

S

G

G

Y

Y

I

I

Y
|
Y

A

S

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

S

D

T

M

E

F

V

E

E

K

N

R

K

M

L

C

A

M

L

L

L

E

E

G

G

A

G

E

E

D

A

A

A

R

V

A

V

T

A

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

S

A

A

A

A

S

I

L

L

W

C

S

Q

A

V

L

K

K

A

S

G

G

D

D

H

H

I

V

V

V

A

A

A

S

R

D

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

F

W

A

V

L

E

N

T

H

L

G

A

L

P

T

K

R

Y

Y

G

G

V

V

E

E

C

V

T

T

L

F

V

V

D

D

G

V

R

S

D

N

L

L

K

D

N

E

W

V

E

K

D

N

A

A

V

L

R

K

P

P

N

N

T

T

K

K

V

V

F

V

F

Y

L

L

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

K

V

V

I

T

D

D

I

I

A

R

G

K

V

I

A

S

R

N

L

M

A

V

D

H

Q

E

I

S

G

N

A

K

K

E

-

C

-

F

V

V

V

F

V

V

D
|
D

N

N

V

T

F
|
F

A

C

T

T

P

P

L

Y

F

I

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

V

V

V

Y

H

S
|
S

A

A

T
|
T

K
|
K

H

Y

I

L

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

A

G

G

V

F

V

A

L

V

S

G

D

K

K

E

Q

E

W

F

I

I

D

N

E

Q

-

V

N

K

L

L

H

F

D

G

Y

I

F

K

R

D

H

M

T

T

G

G

P

S

A

V

M

T

S

G

P

P

F

F

N

E

A

S

W

F

T

L

L

I

K

R

G

G

I

M

E

K

T

T

L

L

P

Q

L

L

R

R

V

M

R

E

Q

K

Q

H

T

C

E

K

S

N

A

A

R

M

R

E

I

V

A

A

D

K

F

F

L

L

T

E

E

S

Q

H

P

P

Q

A

V

V

A

E

R

K

V

V

I

Y

Y

Y

P

P

G

G

R

L

K

E

D

S

H

F

P

K

Q

Y

A

Y

D

Q

I

L

I

A

A

R

K

E

Q

Q

M

M

S

K

G

L

G

P

S

G

T

A

L

M

V

I

A

S

F

F

E

E

L

L

K

K

G

G

K

V

E

E

A

E

A

G

F

K

A

I

L

V

Q

M

N

N

A

N

L

V

E

K

I

L

V

A

R

T

I

L

S

A

N

V

N

S

L

L

G

G

D

D

S

S

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

P

L

Y

T

T

D

A

E

E

A

E

R

R

A

K

E

A

L

A

G

G

I

I

S

S

A

D

G

G

T

L

V

V

R

R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

A

E

E

D

D

L

I

V

I

E

D

D

D

F

L

A

K

K

Q

A

A

L

L

active site: R59 (= R57), Y112 (≠ F110), D186 (= D182), K211 (= K207)
binding pyridoxal-5'-phosphate: Y57 (= Y55), R59 (= R57), S86 (= S84), G87 (= G85), M88 (= M86), Y112 (≠ F110), D186 (= D182), F189 (= F185), S208 (= S204), T210 (= T206), K211 (= K207)

3vk3A Crystal structure of l-methionine gamma-lyase from pseudomonas putida c116h mutant complexed with l-methionine (see paper)
40% identity, 96% coverage: 13:391/394 of query aligns to 16:395/397 of 3vk3A

query
sites
3vk3A

H

H

G

G

G

Y

T

D

L

P

R

Q

S

D

Q

H

Y

G

G

G

E

A

T

L

S

V

E

P

A

P

I

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

T

T

S

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

T

Q

D

A

G

G

Y

H

I

F

Y

Y

A

S

R
|
R

Y

I

G

S

S

N

P

P

T

T

N

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

M

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G

G

M

M

A

G

A

A

V

I

S

T

A

S

A

T

I

L

L

W

C

T

Q

L

V

L

K

R

A

P

G

G

D

D

H

E

I

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

H

C

T

R

F

W

A

V

F

V

L

E

H

T

H

L

G

A

I

P

G

K

E

Y

F

G

G

V

V

E

K

C

L

T

R

L

H

V

V

D

D

G

M

R

A

D

D

L

L

K

Q

N

A

W

L

E

E

D

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

G

G

V

V

A

A

R

K

L

I

A

A

D

R

Q

K

I

H

G

G

A

A

K

T

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S

S

A

A

T

T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

V

V

L

V

S

G

D

S

K

Q

Q

A

W

L

I

V

D

D

E

R

-

I

N

R

L

L

H

Q

D

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

A

V

M

L

S

S

P

P

F

H

N

D

A

A

W

A

T

L

L

L

L

M

K

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

R

Q

R

V

I

L

A

A

D

E

F

F

L

L

T

A

E

R

Q

Q

P

P

Q

Q

V

V

A

E

R

L

V

I

I

H

Y

Y

P

P

G

G

R

L

K

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

K

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

V

I

A

A

F

F

E

E

L

L

K

K

G

G

K

I

E

G

A

A

A

G

F

R

A

R

L

F

Q

M

N

N

A

A

L

L

E

Q

I

L

V

F

R

S

I

R

S

A

N
x
V

N
x
S

L
|
L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

E

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

A

E

G

G

T

L

V

V

R
|
R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

I

E

D

D

D

L

L

V

L

E

A

D

D

F

V

A

Q

K

Q

A

A

L

L

R

K

S

A

active site: R60 (= R57), Y113 (≠ F110), D185 (= D182), K210 (= K207)
binding methionine: Y113 (≠ F110), K210 (= K207), V338 (≠ N334), S339 (≠ N335), L340 (= L336), R374 (= R370)

5x2xA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-homocysteine intermediates (see paper)
40% identity, 96% coverage: 13:391/394 of query aligns to 11:390/392 of 5x2xA

query
sites
5x2xA

H

H

G

G

G

Y

T

D

L

P

R

Q

S

D

Q

H

Y

G

G

G

E

A

T

L

S

V

E

P

A

P

I

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

T

T

S

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

T

Q

D

A

G

G

Y

H

I

F

Y
|
Y

A

S

R
|
R

Y

I

G

S

S

N

P

P

T

T

N

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

M

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G
|
G

M
|
M

A

G

A

A

V

I

S

T

A

S

A

T

I

L

L

W

C

T

Q

L

V

L

K

R

A

P

G

G

D

D

H

E

I

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

F

W

A

V

F

V

L

E

H

T

H

L

G

A

I

P

G

K

E

Y

F

G

G

V

V

E

K

C

L

T

R

L

H

V

V

D

D

G

M

R

A

D

D

L

L

K

Q

N

A

W

L

E

E

D

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N
|
N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

G

G

V

V

A

A

R

K

L

I

A

A

D

R

Q

K

I

H

G

G

A

A

K

T

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

A

A

T
|
T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

V

V

L

V

S

G

D

S

K

Q

Q

A

W

L

I

V

D

D

E

R

-

I

N

R

L

L

H

Q

D

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

A

V

M

L

S

S

P

P

F

H

N

D

A

A

W

A

T

L

L

L

L

M

K

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

R

Q

R

V

I

L

A

A

D

E

F

F

L

L

T

A

E

R

Q

Q

P

P

Q

Q

V

V

A

E

R

L

V

I

I

H

Y

Y

P

P

G

G

R

L

K

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

K

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

V

I

A

A

F

F

E

E

L

L

K

K

G

G

K

I

E

G

A

A

A

G

F

R

A

R

L

F

Q

M

N

N

A

A

L

L

E

Q

I

L

V

F

R

S

I

R

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

E

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

A

E

G

G

T

L

V

V

R
|
R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

I

E

D

D

D

L

L

V

L

E

A

D

D

F

V

A

Q

K

Q

A

A

L

L

R

K

S

A

active site: R55 (= R57), Y108 (≠ F110), D180 (= D182), K205 (= K207)
binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: Y53 (= Y55), R55 (= R57), G83 (= G85), M84 (= M86), Y108 (≠ F110), N155 (= N157), D180 (= D182), S202 (= S204), T204 (= T206), K205 (= K207), V333 (≠ N334), S334 (≠ N335), R369 (= R370)

5x2wA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-methionine intermediates (see paper)
40% identity, 96% coverage: 13:391/394 of query aligns to 11:390/392 of 5x2wA

query
sites
5x2wA

H

H

G

G

G

Y

T

D

L

P

R

Q

S

D

Q

H

Y

G

G

G

E

A

T

L

S

V

E

P

A

P

I

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

T

T

S

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

T

Q

D

A

G

G

Y

H

I

F

Y
|
Y

A

S

R
|
R

Y

I

G

S

S

N

P

P

T

T

N

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

M

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

S

T

A

S

A

T

I

L

L

W

C

T

Q

L

V

L

K

R

A

P

G

G

D

D

H

E

I

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

F

W

A

V

F

V

L

E

H

T

H

L

G

A

I

P

G

K

E

Y

F

G

G

V

V

E

K

C

L

T

R

L

H

V

V

D

D

G

M

R

A

D

D

L

L

K

Q

N

A

W

L

E

E

D

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

G

G

V

V

A

A

R

K

L

I

A

A

D

R

Q

K

I

H

G

G

A

A

K

T

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

A

A

T

T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

V

V

L

V

S

G

D

S

K

Q

Q

A

W

L

I

V

D

D

E

R

-

I

N

R

L

L

H

Q

D

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

A

V

M

L

S

S

P

P

F

H

N

D

A

A

W

A

T

L

L

L

L

M

K

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

R

Q

R

V

I

L

A

A

D

E

F

F

L

L

T

A

E

R

Q

Q

P

P

Q

Q

V

V

A

E

R

L

V

I

I

H

Y

Y

P

P

G

G

R

L

K

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

K

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

V

I

A

A

F

F

E

E

L

L

K

K

G

G

K

I

E

G

A

A

A

G

F

R

A

R

L

F

Q

M

N

N

A

A

L

L

E

Q

I

L

V

F

R

S

I

R

S

A

N
x
V

N
x
S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

E

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

A

E

G

G

T

L

V

V

R
|
R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

I

E

D

D

D

L

L

V

L

E

A

D

D

F

V

A

Q

K

Q

A

A

L

L

R

K

S

A

active site: R55 (= R57), Y108 (≠ F110), D180 (= D182), K205 (= K207)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid: Y53 (= Y55), R55 (= R57), S82 (= S84), G83 (= G85), M84 (= M86), Y108 (≠ F110), D180 (= D182), S202 (= S204), K205 (= K207), V333 (≠ N334), S334 (≠ N335), R369 (= R370)

5x30C Crystal structure of pseudomonas putida methionine gamma-lyase c116h mutant with l-homocysteine intermediates. (see paper)
40% identity, 96% coverage: 13:391/394 of query aligns to 12:391/393 of 5x30C

query
sites
5x30C

H

H

G

G

G

Y

T

D

L

P

R

Q

S

D

Q

H

Y

G

G

G

E

A

T

L

S

V

E

P

A

P

I

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

T

T

S

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

T

Q

D

A

G

G

Y

H

I

F

Y
|
Y

A

S

R
|
R

Y

I

G

S

S

N

P

P

T

T

N

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

M

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G

G

M

M

A

G

A

A

V

I

S

T

A

S

A

T

I

L

L

W

C

T

Q

L

V

L

K

R

A

P

G

G

D

D

H

E

I

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

H

C

T

R

F

W

A

V

F

V

L

E

H

T

H

L

G

A

I

P

G

K

E

Y

F

G

G

V

V

E

K

C

L

T

R

L

H

V

V

D

D

G

M

R

A

D

D

L

L

K

Q

N

A

W

L

E

E

D

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

G

G

V

V

A

A

R

K

L

I

A

A

D

R

Q

K

I

H

G

G

A

A

K

T

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S

S

A

A

T

T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

V

V

L

V

S

G

D

S

K

Q

Q

A

W

L

I

V

D

D

E

R

-

I

N

R

L

L

H

Q

D

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

A

V

M

L

S

S

P

P

F

H

N

D

A

A

W

A

T

L

L

L

L

M

K

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

R

Q

R

V

I

L

A

A

D

E

F

F

L

L

T

A

E

R

Q

Q

P

P

Q

Q

V

V

A

E

R

L

V

I

I

H

Y

Y

P

P

G

G

R

L

K

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

K

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

V

I

A

A

F

F

E

E

L

L

K

K

G

G

K

I

E

G

A

A

A

G

F

R

A

R

L

F

Q

M

N

N

A

A

L

L

E

Q

I

L

V

F

R

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

E

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

A

E

G

G

T

L

V

V

R

R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

I

E

D

D

D

L

L

V

L

E

A

D

D

F

V

A

Q

K

Q

A

A

L

L

R

K

S

A

active site: R56 (= R57), Y109 (≠ F110), D181 (= D182), K206 (= K207)
binding (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid: Y54 (= Y55), R56 (= R57)

1pg8A Crystal structure of l-methionine alpha-, gamma-lyase
40% identity, 96% coverage: 13:391/394 of query aligns to 17:396/398 of 1pg8A

query
sites
1pg8A

H

H

G

G

G

Y

T

D

L

P

R

Q

S

D

Q

H

Y

G

G

G

E

A

T

L

S

V

E

P

A

P

I

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

T

T

S

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

T

Q

D

A

G

G

Y

H

I

F

Y
|
Y

A

S

R
|
R

Y

I

G

S

S

N

P

P

T

T

N

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

M

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

S

T

A

S

A

T

I

L

L

W

C

T

Q

L

V

L

K

R

A

P

G

G

D

D

H

E

I

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

F

W

A

V

F

V

L

E

H

T

H

L

G

A

I

P

G

K

E

Y

F

G

G

V

V

E

K

C

L

T

R

L

H

V

V

D

D

G

M

R

A

D

D

L

L

K

Q

N

A

W

L

E

E

D

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

G

G

V

V

A

A

R

K

L

I

A

A

D

R

Q

K

I

H

G

G

A

A

K

T

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

A

A

T
|
T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

V

V

L

V

S

G

D

S

K

Q

Q

A

W

L

I

V

D

D

E

R

-

I

N

R

L

L

H

Q

D

G

Y

L

F

K

R

D

H

M

T

T

G

G

P

A

A

V

M

L

S

S

P

P

F

H

N

D

A

A

W

A

T

L

L

L

L

M

K

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

R

Q

R

V

I

L

A

A

D

E

F

F

L

L

T

A

E

R

Q

Q

P

P

Q

Q

V

V

A

E

R

L

V

I

I

H

Y

Y

P

P

G

G

R

L

K

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

K

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

V

I

A

A

F

F

E

E

L

L

K

K

G

G

K

I

E

G

A

A

A

G

F

R

A

R

L

F

Q

M

N

N

A

A

L

L

E

Q

I

L

V

F

R

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

E

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

A

E

G

G

T

L

V

V

R

R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

I

E

D

D

D

L

L

V

L

E

A

D

D

F

V

A

Q

K

Q

A

A

L

L

R

K

S

A

active site: R61 (= R57), Y114 (≠ F110), D186 (= D182), K211 (= K207)
binding pyridoxal-5'-phosphate: Y59 (= Y55), R61 (= R57), S88 (= S84), G89 (= G85), M90 (= M86), Y114 (≠ F110), D186 (= D182), S208 (= S204), T210 (= T206), K211 (= K207)

P13254 L-methionine gamma-lyase; MGL; Homocysteine desulfhydrase; L-methioninase; EC 4.4.1.11; EC 4.4.1.2 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
40% identity, 96% coverage: 13:391/394 of query aligns to 17:396/398 of P13254

query
sites
P13254

H

H

G

G

G

Y

T

D

L

P

R

Q

S

D

Q

H

Y

G

G

G

E

A

T

L

S

V

E

P

A

P

I

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

T

T

S

V

E

E

A

Y

A

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

T

Q

D

A

G

G

Y

H

I

F

Y
|
Y

A
x
S

R
|
R

Y

I

G

S

S

N

P

P

T

T

N

L

D

N

M

L

F

L

E

E

K

A

R

R

M

M

C

A

M

S

L

L

E

E

G

G

A

G

E

E

D

A

A

G

R

L

A

A

T

L

A

A

S

S

G
|
G

M
|
M

A

G

A

A

V

I

S

T

A

S

A

T

I

L

L

W

C

T

Q

L

V

L

K

R

A

P

G

G

D

D

H

E

I

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S
x
C

C

T

R

F

W

A

V

F

V

L

E

H

T

H

L

G

A

I

P

G

K

E

Y

F

G

G

V

V

E

K

C

L

T

R

L

H

V

V

D

D

G

M

R

A

D

D

L

L

K

Q

N

A

W

L

E

E

D

A

A

A

V

M

R

T

P

P

N

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

V

M

I

A

D

D

I

I

A

A

G

G

V

V

A

A

R

K

L

I

A

A

D

R

Q

K

I

H

G

G

A

A

K

T

V

V

D

D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

F

L

Q

Q

K

R

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

L

V

V

Y

H

S
|
S

A
|
A

T
|
T

K
|
K

H

Y

I

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

V

V

L

V

S

G

D

S

K

Q

Q

A

W

L

I

V

D

D

E

R

-

I

N

R

L

L

H

Q

D

G

Y

L

F
x
K

R
x
D

H

M

T

T

G

G

P

A

A

V

M

L

S

S

P

P

F

H

N

D

A

A

W

A

T

L

L

L

L

M

K

R

G

G

I

I

E

K

T

T

L

L

P

N

L

L

R

R

V

M

R

D

Q

R

Q

H

T

C

E

A

S

N

A

A

R

Q

R

V

I

L

A

A

D

E

F

F

L

L

T

A

E

R

Q

Q

P

P

Q

Q

V

V

A

E

R

L

V

I

I

H

Y

Y

P

P

G

G

R

L

K

A

D

S

H

F

P

P

Q

Q

A

Y

D

T

I

L

I

A

A

R

K

Q

Q

Q

M

M

S

S

G

Q

G

P

S

G

T

G

L

M

V

I

A

A

F

F

E

E

L

L

K

K

G

G

K

I

E

G

A

A

A

G

F

R

A

R

L

F

Q

M

N

N

A

A

L

L

E

Q

I

L

V

F

R

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

S

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

S

N

S

L

Y

T

T

D

P

E

E

A

E

R

R

A

A

E

H

L

Y

G

G

I

I

S

S

A

E

G

G

T

L

V

V

R
|
R

F

L

S

S

A

V

G

G

I

L

E

E

D

D

S

I

E

D

D

D

L

L

V

L

E

A

D

D

F

V

A

Q

K

Q

A

A

L

L

R

K

S

A

YSR 59:61 (≠ YAR 55:57) binding pyridoxal 5'-phosphate
R61 (= R57) mutation R->A,E,F: Loss of elimination activity against L-methionine.
GM 89:90 (= GM 85:86) binding in other chain
Y114 (≠ F110) binding substrate
C116 (≠ S112) mutation to H: Drastic decrease of the catalytic efficiency of the elimination reaction with L-methionine, by 6700-fold, and increases that with L-cysteine by 7-fold, mainly due to changes in kcat. Loss of ability to catalyze replacement reaction between L-methionine and 2-mercaptoethanol.; mutation to S: 9% of wild-type elimination activity against L-methionine.; mutation to T: 40% of wild-type elimination activity against L-methionine.
SAT 208:210 (= SAT 204:206) binding in other chain
K211 (= K207) modified: N6-(pyridoxal phosphate)lysine
K240 (≠ F235) mutation K->D,E: Marked decrease in elimination activity against both L-methionine and DL-homocysteine.; mutation to M: 50% reduction in alpha,gamma-elimination activity against DL-homocysteine, while retaining elimination activity against L-methionine and L-cysteine.
D241 (≠ R236) mutation D->H,R: 5 to 14-fold reduction in alpha,gamma-elimination activity against L-methionine, while no change in affinity for L-methionine.
R375 (= R370) binding substrate

1e5eA Methionine gamma-lyase (mgl) from trichomonas vaginalis in complex with propargylglycine
35% identity, 98% coverage: 7:392/394 of query aligns to 5:392/394 of 1e5eA

query
sites
1e5eA

P

P

A

A

T

T

Q

A

L

C

V

I

H

H

G

A

G

N

T

P

L

Q

R

K

S

D

Q

Q

Y

F

G

G

E

A

T

A

S

I

E

P

A

P

I

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

T

N

S

C

E

Q

A

Q

A

G

E

G

A

N

R

R

F

F

K

A

G

G

E

Q

T

E

D

S

G

G

Y

Y

I

I

Y
|
Y

A

T

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

V

D

S

M

N

F

L

E

E

K

G

R

K

M

I

C

A

M

F

L

L

E

E

G

K

A

T

E

E

D

A

A

C

R

V

A

A

T

T

A

S

S

S

G
|
G

M
|
M

A

G

A

A

V

I

S

A

A

A

A

T

I

V

L

L

C

T

Q

I

V

L

K

K

A

A

G

G

D

D

H

H

I

L

V

I

A

S

A

D

R

E

A

C

L

L

F
x
Y

G

G

S

C

C

T

R

H

W

A

V

L

V

F

E

E

T

H

L

A

A

L

P

T

K

K

Y

F

G

G

V

I

E

Q

C

V

T

D

L

F

V

I

D

N

G

T

R

A

D

I

L

P

K

G

N

E

W

V

E

K

D

K

A

H

V

M

R

K

P

P

N

N

T

T

K

K

V

I

F

V

F

Y

L

F

E

E

S

T

P

P

T

A

N
|
N

P

P

T

T

L

L

E

K

V

I

I

I

D

D

I

M

A

E

G

R

V

V

A

C

R

K

L

D

A

A

-

H

D

S

Q

Q

I

E

G

G

A

V

K

L

V

V

V

I

V

A

D
|
D

N

N

V

T

F

F

A

C

T

S

P

P

L

M

F

I

Q

T

K

N

P

P

L

V

E

D

L

F

G

G

A

V

H

D

I

V

V

V

Y

H

S
|
S

A

A

T
|
T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

T

R

D

C

V

L

V

G

A

G

G

V

L

V

I

L

C

S

G

D

K

K

A

Q

D

W

L

I

L

D

Q

E

Q

-

I

-

R

-

M

-

V

N

G

L

I

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

S

A

V

M

I

S

S

P

P

F

H

N

D

A

A

W

W

T

L

L

I

L

T

K

R

G

G

I

L

E

S

T

T

L

L

P

N

L

I

R

R

V

M

R

K

Q

A

Q

E

T

S

E

E

S

N

A

A

R

M

R

K

I

V

A

A

D

E

F

Y

L

L

T

K

E

S

Q

H

P

P

Q

A

V

V

A

E

R

K

V

V

I

Y

Y

Y

P

P

G

G

R

F

K

E

D

D

H

H

P

E

Q

G

A

H

D

D

I

I

I

A

A

K

K

K

Q

Q

M

M

S

R

G

M

G

Y

S

G

T

S

L

M

V

I

A

T

F

F

E

I

L

L

K

K

G

S

G

G

K

F

E

E

A

G

A

A

F

K

A

K

L

L

Q

L

N

D

A

N

L

L

E

K

I

L

V

I

R

T

I

L

S

A

N

V

N
x
S

L

L

G

G

D

G

S

C

K

E

S

S

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T
|
T

H

H

K

A

N

V

L

V

T

P

D

K

E

E

A

E

R

R

A

E

E

A

L

A

G

G

I

I

S

T

A

D

G

G

T

M

V

I

R
|
R

F

L

S

S

A

V

G

G

I

I

E

E

D

D

S

A

E

D

D

E

L

L

V

I

E

A

D

D

F

F

A

K

K

Q

A

G

L

L

R

D

S

A

V

L

active site: R55 (= R57), Y108 (≠ F110), D181 (= D182), K206 (= K207)
binding n-(hydroxy{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)norvaline: Y53 (= Y55), R55 (= R57), G83 (= G85), M84 (= M86), Y108 (≠ F110), N155 (= N157), D181 (= D182), S203 (= S204), T205 (= T206), K206 (= K207), S335 (≠ N335), T350 (= T350), R370 (= R370)

1e5fA Methionine gamma-lyase (mgl) from trichomonas vaginalis
35% identity, 98% coverage: 7:392/394 of query aligns to 5:392/393 of 1e5fA

query
sites
1e5fA

P

P

A

A

T

T

Q

A

L

C

V

I

H

H

G

A

G

N

T

P

L

Q

R

K

S

D

Q

Q

Y

F

G

G

E

A

T

A

S

I

E

P

A

P

I

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

T

N

S

C

E

Q

A

Q

A

G

E

G

A

N

R

R

F

F

K

A

G

G

E

Q

T

E

D

S

G

G

Y

Y

I

I

Y
|
Y

A

T

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

V

D

S

M

N

F

L

E

E

K

G

R

K

M

I

C

A

M

F

L

L

E

E

G

K

A

T

E

E

D

A

A

C

R

V

A

A

T

T

A

S

S

S

G
|
G

M
|
M

A

G

A

A

V

I

S

A

A

A

A

T

I

V

L

L

C

T

Q

I

V

L

K

K

A

A

G

G

D

D

H

H

I

L

V

I

A

S

A

D

R

E

A

C

L

L

F
x
Y

G

G

S

C

C

T

R

H

W

A

V

L

V

F

E

E

T

H

L

A

A

L

P

T

K

K

Y

F

G

G

V

I

E

Q

C

V

T

D

L

F

V

I

D

N

G

T

R

A

D

I

L

P

K

G

N

E

W

V

E

K

D

K

A

H

V

M

R

K

P

P

N

N

T

T

K

K

V

I

F

V

F

Y

L

F

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

K

V

I

I

I

D

D

I

M

A

E

G

R

V

V

A

C

R

K

L

D

A

A

-

H

D

S

Q

Q

I

E

G

G

A

V

K

L

V

V

V

I

V

A

D
|
D

N

N

V

T

F

F

A

C

T

S

P

P

L

M

F

I

Q

T

K

N

P

P

L

V

E

D

L

F

G

G

A

V

H

D

I

V

V

V

Y

H

S
|
S

A

A

T

T

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

T

R

D

C

V

L

V

G

A

G

G

V

L

V

I

L

C

S

G

D

K

K

A

Q

D

W

L

I

L

D

Q

E

Q

-

I

-

R

-

M

-

V

N

G

L

I

H

K

D

D

Y

I

F

-

R

-

H

-

T

T

G

G

P

S

A

V

M

I

S

S

P

P

F

H

N

D

A

A

W

W

T

L

L

I

L

T

K

R

G

G

I

L

E

S

T

T

L

L

P

N

L

I

R

R

V

M

R

K

Q

A

Q

E

T

S

E

E

S

N

A

A

R

M

R

K

I

V

A

A

D

E

F

Y

L

L

T

K

E

S

Q

H

P

P

Q

A

V

V

A

E

R

K

V

V

I

Y

Y

Y

P

P

G

G

R

F

K

E

D

D

H

H

P

E

Q

G

A

H

D

D

I

I

I

A

A

K

K

K

Q

Q

M

M

S

R

G

M

G

Y

S

G

T

S

L

M

V

I

A

T

F

F

E

I

L

L

K

K

G

S

G

G

K

F

E

E

A

G

A

A

F

K

A

K

L

L

Q

L

N

D

A

N

L

L

E

K

I

L

V

I

R

T

I

L

S

A

N

V

N

S

L

L

G

G

D

G

S

C

K

E

S

S

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

K

A

N

V

L

V

T

P

D

K

E

E

A

E

R

R

A

E

E

A

L

A

G

G

I

I

S

T

A

D

G

G

T

M

V

I

R

R

F

L

S

S

A

V

G

G

I

I

E

E

D

D

S

A

E

D

D

E

L

L

V

I

E

A

D

D

F

F

A

K

K

Q

A

G

L

L

R

D

S

A

V

L

active site: R55 (= R57), Y108 (≠ F110), D181 (= D182), K206 (= K207)
binding pyridoxal-5'-phosphate: Y53 (= Y55), R55 (= R57), G83 (= G85), M84 (= M86), Y108 (≠ F110), D181 (= D182), S203 (= S204), K206 (= K207)

3aeoA Reaction intermediate structure of entamoeba histolytica methionine gamma-lyase 1 containing methionine alpha, beta-enamine-pyridoxamine- 5'-phosphate
37% identity, 92% coverage: 28:390/394 of query aligns to 24:385/387 of 3aeoA

query
sites
3aeoA

I

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

C

Y

F

D

D

T

S

S

T

E

Q

A

Q

A

G

E

A

A

D

R

L

F

F

K

M

G

G

E

K

T

G

D

E

G

G

Y

H

I

I

Y
|
Y

A

S

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

V

D

E

M

Q

F

F

E

E

K

E

R

M

M

V

C

C

M

S

L

I

E

E

G

G

A

A

E

A

D

G

A

S

R

A

A

A

T

F

A

G

S

S

G
|
G

M
|
M

A

G

A

A

V

I

S

S

A

S

I

T

L

L

C

A

Q

F

V

L

K

Q

A

K

G

G

D

D

H

H

I

L

V

I

A

A

A

G

R

D

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

V

W

S

V

L

V

F

E

T

T

H

L

W

A

L

P

P

K

R

Y

F

G

G

V

I

E

E

C

V

T

D

L

L

V

I

D

D

G

T

R

S

D

D

L

V

K

E

N

K

W

V

E

K

D

A

A

A

V

W

R

K

P

P

N

N

T

T

K

K

V

M

F

V

F

Y

L

L

E
|
E

S

S

P

P

T

A

N
|
N

P

P

T

T

L

C

E

K

V

V

I

S

D

D

I

I

A

K

G

G

V

I

A

A

R

V

L

V

A

C

D

H

Q

E

I

R

G

G

A

A

K

R

V

L

V

V

D
|
D

N

A

V

T

F

F

A

T

T

S

P

P

L

C

F

F

Q

L

K

K

P

P

L

L

E

E

L

L

G

G

A

A

H

D

I

I

V

A

V

L

Y

H

S
|
S

A

V

T
x
S

K
|
K

H

Y

I

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

V

V

S

L

S

S

A

D

K

K

T

Q

A

W

E

I

D

D

I

E

A

N

T

L

I

H

K

D

F

Y

Y

F

R

R

K

H

D

T

A

G

G

P

S

A

L

M

M

S

A

P

P

F

M

N

D

A

A

W

F

T

L

L

C

L

A

K

R

G

G

I

M

E

K

T

T

L

L

P

P

L

I

R

R

V

M

R

Q

Q

I

Q

H

T

M

E

E

S

N

A

G

R

L

R

K

I

V

A

A

D

K

F

F

L

L

T

E

E

Q

Q

H

P

E

Q

K

V

I

A

V

R

K

V

V

I

N

Y

H

P

P

G

G

R

L

K

E

D

S

H

F

P

P

Q

G

A

H

D

D

I

I

I

A

A

K

K

K

Q

Q

M

M

S

T

G

G

G

Y

S

G

T

S

L

T

V

F

A

L

F

F

E

E

L

M

K

K

G

-

G

S

K

F

E

E

A

A

F

K

A

K

L

L

Q

M

N

E

A

H

L

L

E

K

I

V

V

C

R

T

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

C

S

V

K

D

S

T

L

L

I

I

T

E

H

H

P

P

A

A

T

S

T

M

T
|
T

H

H

K

A

N

A

L

V

T

P

D

E

E

N

A

I

R

M

A

R

E

K

L

Q

G

G

I

I

S

T

A

P

G

E

T

L

V

V

R
|
R

F

I

S

S

A

V

G

G

I

I

E

E

D

N

S

V

E

D

D

D

L

I

V

I

E

A

D

D

F

L

A

K

K

Q

A

A

L

L

R

E

binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid: Y51 (= Y55), R53 (= R57), G81 (= G85), M82 (= M86), Y106 (≠ F110), E149 (= E153), N153 (= N157), D178 (= D182), S200 (= S204), S202 (≠ T206), K203 (= K207), V329 (≠ N334), S330 (≠ N335), T345 (= T350), R365 (= R370)

3aelA Reaction intermediate structure of entamoeba histolytica methionine gamma-lyase 1 containing methionine imine-pyridoxamine-5'-phosphate and alpha-amino-alpha, beta-butenoic acid-pyridoxal-5'-phosphate
37% identity, 92% coverage: 28:390/394 of query aligns to 24:385/387 of 3aelA

query
sites
3aelA

I

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

C

Y

F

D

D

T

S

S

T

E

Q

A

Q

A

G

E

A

A

D

R

L

F

F

K

M

G

G

E

K

T

G

D

E

G

G

Y

H

I

I

Y
|
Y

A

S

R
|
R

Y

L

G

G

S

N

P

P

T

T

N

V

D

E

M

Q

F

F

E

E

K

E

R

M

M

V

C

C

M

S

L

I

E

E

G

G

A

A

E

A

D

G

A

S

R

A

A

A

T

F

A

G

S

S

G
|
G

M
|
M

A

G

A

A

V

I

S

S

A

S

I

T

L

L

C

A

Q

F

V

L

K

Q

A

K

G

G

D

D

H

H

I

L

V

I

A

A

A

G

R

D

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

V

W

S

V

L

V

F

E

T

T

H

L

W

A

L

P

P

K

R

Y

F

G

G

V

I

E

E

C

V

T

D

L

L

V

I

D

D

G

T

R

S

D

D

L

V

K

E

N

K

W

V

E

K

D

A

A

A

V

W

R

K

P

P

N

N

T

T

K

K

V

M

F

V

F

Y

L

L

E
|
E

S

S

P

P

T

A

N
|
N

P

P

T

T

L

C

E

K

V

V

I

S

D

D

I

I

A

K

G

G

V

I

A

A

R

V

L

V

A

C

D

H

Q

E

I

R

G

G

A

A

K

R

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binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)imino]-4-(methylsulfanyl)butanoic acid: Y51 (= Y55), R53 (= R57)
binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: G81 (= G85), M82 (= M86), Y106 (≠ F110), E149 (= E153), N153 (= N157), D178 (= D182), T180 (≠ V184), S200 (= S204), S202 (≠ T206), K203 (= K207), S330 (≠ N335), T345 (= T350), R365 (= R370)

Query Sequence

>WP_011974345.1 NCBI__GCF_000017145.1:WP_011974345.1
MSKNWRPATQLVHGGTLRSQYGETSEAIFLTQGFVYDTSEAAEARFKGETDGYIYARYGS
PTNDMFEKRMCMLEGAEDARATASGMAAVSAAILCQVKAGDHIVAARALFGSCRWVVETL
APKYGVECTLVDGRDLKNWEDAVRPNTKVFFLESPTNPTLEVIDIAGVARLADQIGAKVV
VDNVFATPLFQKPLELGAHIVVYSATKHIDGQGRCLGGVVLSDKQWIDENLHDYFRHTGP
AMSPFNAWTLLKGIETLPLRVRQQTESARRIADFLTEQPQVARVIYPGRKDHPQADIIAK
QMSGGSTLVAFELKGGKEAAFALQNALEIVRISNNLGDSKSLITHPATTTHKNLTDEARA
ELGISAGTVRFSAGIEDSEDLVEDFAKALRSVTA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory