SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011975013.1 NCBI__GCF_000017145.1:WP_011975013.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
62% identity, 95% coverage: 12:422/431 of query aligns to 2:404/405 of 1kl2A

query
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1kl2A

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active site: Y51 (= Y61), E53 (= E63), D197 (= D207), T223 (= T233), K226 (= K236), R232 (= R242)
binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E63), Y60 (= Y70), G121 (= G131), H122 (= H132), S172 (≠ T182), F251 (= F262), N341 (= N352)
binding glycine: S31 (= S41), Y51 (= Y61), Y61 (= Y71), H200 (= H210), R357 (= R368)
binding pyridoxal-5'-phosphate: S93 (= S103), G94 (= G104), A95 (≠ S105), H122 (= H132), S172 (≠ T182), D197 (= D207), A199 (= A209), H200 (= H210), T223 (= T233), H225 (= H235), K226 (= K236)

1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
62% identity, 95% coverage: 12:422/431 of query aligns to 2:404/405 of 1kl1A

query
sites
1kl1A

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active site: Y51 (= Y61), E53 (= E63), D197 (= D207), T223 (= T233), K226 (= K236), R232 (= R242)
binding glycine: S31 (= S41), H122 (= H132), R357 (= R368)
binding pyridoxal-5'-phosphate: S93 (= S103), G94 (= G104), A95 (≠ S105), H122 (= H132), A171 (≠ G181), S172 (≠ T182), D197 (= D207), A199 (= A209), H200 (= H210), T223 (= T233), H225 (= H235), K226 (= K236)

1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
62% identity, 95% coverage: 12:422/431 of query aligns to 2:404/405 of 1kkpA

query
sites
1kkpA

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G

E

N

Q

A

A

A

L

V

E

E

E

A

A

G

-

V

-

R

R

E

Q

K

R

V

V

M

A

K

A

L

L

T

T

active site: Y51 (= Y61), E53 (= E63), D197 (= D207), T223 (= T233), K226 (= K236), R232 (= R242)
binding pyridoxal-5'-phosphate: S93 (= S103), G94 (= G104), A95 (≠ S105), H122 (= H132), S172 (≠ T182), D197 (= D207), A199 (= A209), H200 (= H210), K226 (= K236)
binding serine: S31 (= S41), H122 (= H132), R357 (= R368)

1kkjA Crystal structure of serine hydroxymethyltransferase from b.Stearothermophilus (see paper)
62% identity, 95% coverage: 12:422/431 of query aligns to 2:404/405 of 1kkjA

query
sites
1kkjA

R

K

S

Y

L

L

A

P

D

Q

S

Q

D

D

P

P

D

Q

I

V

F

F

G

A

A

A

I

I

E

E

K

Q

E

E

L

R

G

K

R

R

Q

Q

R

H

H

A

E

K

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

I

F

V

V

S

S

R

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

Q

E

Y

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

E

E

R

R

A

A

K

K

K

Q

L

L

F

F

G

G

V

A

G

E

F

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

M

A

N

N

Q

M

A

A

V

V

F

Y

L

F

A

T

L

V

L

L

Q

E

P

H

G

G

D

D

T

T

F

V

M

L

G

G

L

M

D

N

L

L

N

S

S

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

V

V

R

D

E

P

D

E

D

T

H

H

L

V

L

I

D

D

M

Y

D

D

D

D

V

V

A

R

K

E

K

K

A

A

R

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

I

V

A

A

G

A

G

A

T
x
S

A

A

Y

Y

S

P

R

R

I

I

W

I

D

D

W

F

K

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

G

A

D

G

Q

L

H

H

P

P

S

N

P

P

F

V

P

P

H

Y

C

A

H

H

V

F

A

V

T

T

T

T

T
|
T

T

T

H
|
H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

M

M

I

I

L

L

T

C

N

Q

D

-

E

E

E

Q

I

F

A

A

K

K

K

Q

I

I

N

D

S

K

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

Q

Q

P

D

S

D

F

F

K

K

D

A

Y

Y

A

A

A

K

Q

R

V

V

V

V

K

D

N

N

A

A

R

K

T

R

L

L

A

A

D

S

T

A

L

L

K

Q

A

N

N

E

G

G

L

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

K

P

K

Q

N

Q

A

L

T

T

G

G

K

K

R

T

A

A

E

E

A

K

A

V

L

L

G

D

R

E

A

V

Y

G

V

I

T

T

C

V

N

N

K

K

N

N

G

T

I

I

P

P

F

Y

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R

R

L

I

G

G

T

T

P

A

A

A

G

V

T

T

T

T

R

R

G

G

F

F

K

G

E

L

A

E

E

E

F

M

K

D

E

E

I

I

G

A

E

A

L

I

I

I

V

-

E

-

V

-

L

-

D

-

G

G

L

L

K

V

A

L

A

K

N

N

S

V

D

G

E

S

G

E

N

Q

A

A

A

L

V

E

E

E

A

A

G

-

V

-

R

R

E

Q

K

R

V

V

M

A

K

A

L

L

T

T

active site: Y51 (= Y61), E53 (= E63), D197 (= D207), T223 (= T233), K226 (= K236), R232 (= R242)
binding pyridoxal-5'-phosphate: S93 (= S103), G94 (= G104), A95 (≠ S105), H122 (= H132), S172 (≠ T182), D197 (= D207), A199 (= A209), H200 (= H210), T223 (= T233), H225 (= H235), K226 (= K236)

4otlA X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and glycine
64% identity, 97% coverage: 13:428/431 of query aligns to 2:408/409 of 4otlA

query
sites
4otlA

S

T

L

I

A

A

D

N

S

V

D

D

P

P

D

E

I

I

F

F

G

A

A

A

I

I

E

E

K

Q

E

E

L

N

G

R

R

R

Q

Q

R

E

H

D

E

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

Y

V

T

S

S

R

P

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

K

R

R

Y

Y

Y
|
Y

G

G

G

G

C

C

Q

E

Y

Y

V

V

D

D

I

V

A

V

E

E

E

Q

L

L

A

A

I

I

E

D

R

R

A

V

K

K

K

Q

L

L

F

F

G

G

V

A

G

E

F

A

A

A

N

N

V

V

Q

Q

P

P

N

N

S
|
S

G
|
G

S
|
S

Q

Q

M

A

N

N

Q

Q

A

G

V

V

F

F

L

F

A

A

L

M

L

L

Q

K

P

P

G

G

D

D

T

T

F

I

M

M

G

G

L

M

D

S

L

L

N

A

S

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

M

S

S

G

G

K

K

W

W

F

F

N

N

V

V

V

V

S

S

Y

Y

G

G

V

L

R

N

E

E

D

N

D

E

H

D

L

-

L

I

D

D

M

Y

D

D

D

A

V

A

A

E

K

K

K

L

A

A

R

N

E

E

H

H

K

K

P

P

K

K

L

L

I

I

I

V

A

A

G

G

G

A

T
x
S

A

A

Y

F

S

A

R

L

I

K

W

I

D

D

W

F

K

E

R

R

F

L

R

A

E

K

I

I

A

A

D

K

E

S

V

V

G

G

A

A

W

Y

L

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

G

A

D

G

Q

V

H

Y

P

P

S

N

P

P

F

V

P

P

H

H

C

A

H

D

V

F

A

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

S

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

M

V

I

I

L

L

T

M

N

K

D

-

E

A

E

E

I

Y

A

E

K

K

K

P

I

I

N

N

S

S

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

F

F

G

K

E

E

A

A

L

L

Q

S

P

P

S

E

F

F

K

K

D

E

Y

Y

A

Q

A

Q

Q

K

V

V

V

V

K

E

N

N

A

A

R

R

T

V

L

L

A

A

D

E

T

T

L

L

K

V

A

K

N

R

G

G

L

L

D

R

I

I

V

V

S

S

G

G

G

R

T

T

D

E

N

S

H

H

L

V

M

M

L

L

V

V

D

D

L

L

R

R

K

A

K

K

N

H

A

I

T

T

G

G

K

K

R

A

A

A

E

E

A

A

A

A

L

L

G

G

R

A

A

A

Y

H

V

I

T

T

C

V

N

N

K

K

N

N

G

A

I

I

P

P

F

N

D

D

P

P

E

E

K

K

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

L

L

G

G

T

S

P

P

A

A

G

M

T

T

T

T

R

R

G

G

F

F

K

G

E

P

A

A

E

E

F

A

K

E

E

Q

I

V

G

G

E

N

L

L

I

I

V

A

E

D

V

V

L

L

D

E

G

-

L

-

K

-

A

-

A

-

N

-

S

N

D

P

E

E

G

D

N

A

A

A

A

T

V

I

E

E

A

R

G

-

V

V

R

R

E

A

K

Q

V

V

M

A

K

E

L

L

T

T

G

K

R

R

F

F

P

P

M

V

Y

Y

active site: Y50 (= Y61), E52 (= E63), D195 (= D207), T221 (= T233), K224 (= K236), R230 (= R242)
binding glycine: S30 (= S41), Y50 (= Y61), Y60 (= Y71), H121 (= H132), K224 (= K236), R355 (= R368)
binding pyridoxal-5'-phosphate: S92 (= S103), G93 (= G104), S94 (= S105), H121 (= H132), S170 (≠ T182), D195 (= D207), A197 (= A209), H198 (= H210), K224 (= K236)

4ot8A X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and serine
64% identity, 97% coverage: 13:428/431 of query aligns to 7:413/414 of 4ot8A

query
sites
4ot8A

S

T

L

I

A

A

D

N

S

V

D

D

P

P

D

E

I

I

F

F

G

A

A

A

I

I

E

E

K

Q

E

E

L

N

G

R

R

R

Q

Q

R

E

H

D

E

H

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

Y

V

T

S

S

R

P

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

K

R

R

Y

Y

Y
|
Y

G

G

G

G

C

C

Q

E

Y

Y

V

V

D

D

I

V

A

V

E

E

E

Q

L

L

A

A

I

I

E

D

R

R

A

V

K

K

K

Q

L

L

F

F

G

G

V

A

G

E

F

A

A

A

N

N

V

V

Q

Q

P

P

N

N

S
|
S

G
|
G

S
|
S

Q

Q

M

A

N

N

Q

Q

A

G

V

V

F

F

L

F

A

A

L

M

L

L

Q

K

P

P

G

G

D

D

T

T

F

I

M

M

G

G

L

M

D

S

L

L

N

A

S

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

M

S

S

G

G

K

K

W

W

F

F

N

N

V

V

V

V

S

S

Y

Y

G

G

V

L

R

N

E

E

D

N

D

E

H

D

L

-

L

I

D

D

M

Y

D

D

D

A

V

A

A

E

K

K

K

L

A

A

R

N

E

E

H

H

K

K

P

P

K

K

L

L

I

I

I

V

A

A

G

G

G

A

T

S

A

A

Y

F

S

A

R

L

I

K

W

I

D

D

W

F

K

E

R

R

F

L

R

A

E

K

I

I

A

A

D

K

E

S

V

V

G

G

A

A

W

Y

L

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

G

A

D

G

Q

V

H

Y

P

P

S

N

P

P

F

V

P

P

H

H

C

A

H

D

V

F

A

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

S

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

M

V

I

I

L

L

T

M

N

K

D

-

E

A

E

E

I

Y

A

E

K

K

K

P

I

I

N

N

S

S

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

F

F

G

K

E

E

A

A

L

L

Q

S

P

P

S

E

F

F

K

K

D

E

Y

Y

A

Q

A

Q

Q

K

V

V

V

V

K

E

N

N

A

A

R

R

T

V

L

L

A

A

D

E

T

T

L

L

K

V

A

K

N

R

G

G

L

L

D

R

I

I

V

V

S

S

G

G

G

R

T

T

D

E

N

S

H

H

L

V

M

M

L

L

V

V

D

D

L

L

R

R

K

A

K

K

N

H

A

I

T

T

G

G

K

K

R

A

A

A

E

E

A

A

A

A

L

L

G

G

R

A

A

A

Y

H

V

I

T

T

C

V

N

N

K

K

N

N

G

A

I

I

P

P

F

N

D

D

P

P

E

E

K

K

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R
|
R

L

L

G

G

T

S

P

P

A

A

G

M

T

T

T

T

R

R

G

G

F

F

K

G

E

P

A

A

E

E

F

A

K

E

E

Q

I

V

G

G

E

N

L

L

I

I

V

A

E

D

V

V

L

L

D

E

G

-

L

-

K

-

A

-

A

-

N

-

S

N

D

P

E

E

G

D

N

A

A

A

A

T

V

I

E

E

A

R

G

-

V

V

R

R

E

A

K

Q

V

V

M

A

K

E

L

L

T

T

G

K

R

R

F

F

P

P

M

V

Y

Y

active site: Y55 (= Y61), E57 (= E63), D200 (= D207), T226 (= T233), K229 (= K236), R235 (= R242)
binding pyridoxal-5'-phosphate: S97 (= S103), G98 (= G104), S99 (= S105), H126 (= H132), D200 (= D207), A202 (= A209), H203 (= H210), K229 (= K236)
binding serine: S35 (= S41), E57 (= E63), Y65 (= Y71), H126 (= H132), H203 (= H210), R360 (= R368)

4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
64% identity, 97% coverage: 13:428/431 of query aligns to 9:415/416 of 4n0wA

query
sites
4n0wA

S

T

L

I

A

A

D

N

S

V

D

D

P

P

D

E

I

I

F

F

G

A

A

A

I

I

E

E

K

Q

E

E

L

N

G

R

R

R

Q

Q

R

E

H

D

E

H

I

I

E

E

L

L

I

I

A

A

S

S

E

E

N

N

I

Y

V

T

S

S

R

P

A

A

V

V

L

M

E

A

A

A

Q

Q

G

G

S

S

I

Q

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

K

R

R

Y

Y

Y

Y

G

G

G

G

C

C

Q

E

Y

Y

V

V

D

D

I

V

A

V

E

E

E

Q

L

L

A

A

I

I

E

D

R

R

A

V

K

K

K

Q

L

L

F

F

G

G

V

A

G

E

F

A

A

A

N

N

V

V

Q

Q

P

P

N

N

S
|
S

G
|
G

S
|
S

Q

Q

M

A

N

N

Q

Q

A

G

V

V

F

F

L

F

A

A

L

M

L

L

Q

K

P

P

G

G

D

D

T

T

F

I

M

M

G

G

L

M

D

S

L

L

N

A

S

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

M

S

S

G

G

K

K

W

W

F

F

N

N

V

V

V

V

S

S

Y

Y

G

G

V

L

R

N

E

E

D

N

D

E

H

D

L

-

L

I

D

D

M

Y

D

D

D

A

V

A

A

E

K

K

K

L

A

A

R

N

E

E

H

H

K

K

P

P

K

K

L

L

I

I

I

V

A

A

G

G

G

A

T

S

A

A

Y

F

S

A

R

L

I

K

W

I

D

D

W

F

K

E

R

R

F

L

R

A

E

K

I

I

A

A

D

K

E

S

V

V

G

G

A

A

W

Y

L

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

Y

A

A

G

G

L

L

V

I

A

A

G

A

D

G

Q

V

H

Y

P

P

S

N

P

P

F

V

P

P

H

H

C

A

H

D

V

F

A

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

S

S

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

M

V

I

I

L

L

T

M

N

K

D

-

E

A

E

E

I

Y

A

E

K

K

K

P

I

I

N

N

S

S

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

F

F

G

K

E

E

A

A

L

L

Q

S

P

P

S

E

F

F

K

K

D

E

Y

Y

A

Q

A

Q

Q

K

V

V

V

V

K

E

N

N

A

A

R

R

T

V

L

L

A

A

D

E

T

T

L

L

K

V

A

K

N

R

G

G

L

L

D

R

I

I

V

V

S

S

G

G

G

R

T

T

D

E

N

S

H

H

L

V

M

M

L

L

V

V

D

D

L

L

R

R

K

A

K

K

N

H

A

I

T

T

G

G

K

K

R

A

A

A

E

E

A

A

A

A

L

L

G

G

R

A

A

A

Y

H

V

I

T

T

C

V

N

N

K

K

N

N

G

A

I

I

P

P

F

N

D

D

P

P

E

E

K

K

P

P

F

F

V

V

T

T

S

S

G

G

V

I

R

R

L

L

G

G

T

S

P

P

A

A

G

M

T

T

T

T

R

R

G

G

F

F

K

G

E

P

A

A

E

E

F

A

K

E

E

Q

I

V

G

G

E

N

L

L

I

I

V

A

E

D

V

V

L

L

D

E

G

-

L

-

K

-

A

-

A

-

N

-

S

N

D

P

E

E

G

D

N

A

A

A

A

T

V

I

E

E

A

R

G

-

V

V

R

R

E

A

K

Q

V

V

M

A

K

E

L

L

T

T

G

K

R

R

F

F

P

P

M

V

Y

Y

active site: Y57 (= Y61), E59 (= E63), D202 (= D207), T228 (= T233), K231 (= K236), R237 (= R242)
binding pyridoxal-5'-phosphate: S99 (= S103), G100 (= G104), S101 (= S105), H128 (= H132), D202 (= D207), A204 (= A209), H205 (= H210), K231 (= K236)

2vmyA Crystal structure of f351gbsshmt in complex with gly and fthf (see paper)
62% identity, 95% coverage: 12:422/431 of query aligns to 2:404/405 of 2vmyA

query
sites
2vmyA

R

K

S

Y

L

L

A

P

D

Q

S

Q

D

D

P

P

D

Q

I

V

F

F

G

A

A

A

I

I

E

E

K

Q

E

E

L

R

G

K

R

R

Q

Q

R

H

H

A

E

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

V

S

S

R

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y
|
Y

Y
|
Y

G

G

G

G

C

C

Q

E

Y

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

E

E

R

R

A

A

K

K

K

Q

L

L

F

F

G

G

V

A

G

E

F

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

M

A

N

N

Q

M

A

A

V

V

F

Y

L

F

A

T

L

V

L

L

Q

E

P

H

G

G

D

D

T

T

F

V

M

L

G

G

L

M

D

N

L
|
L

N

S

S

H

G

G

G
|
G

H
|
H

L
|
L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

V

V

R

D

E

P

D

E

D

T

H

H

L

V

L

I

D

D

M

Y

D

D

D

D

V

V

A

R

K

E

K

K

A

A

R

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

I

V

A

A

G

A

G

A

T
x
S

A

A

Y

Y

S

P

R

R

I

I

W

I

D

D

W

F

K

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

G

A

D

G

Q

L

H

H

P

P

S

N

P

P

F

V

P

P

H

Y

C

A

H

H

V

F

A

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

M

M

I

I

L

L

T

C

N

Q

D

-

E

E

E

Q

I

F

A

A

K

K

K

Q

I

I

N

D

S
x
K

A

A

V

I

F
|
F

P

P

G

G

L

I

Q

Q

G

G

G
|
G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

Q

Q

P

D

S

D

F

F

K

K

D

A

Y

Y

A

A

A

K

Q

R

V

V

V

V

K

D

N

N

A

A

R

K

T

R

L

L

A

A

D

S

T

A

L

L

K

Q

A

N

N

E

G

G

L

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

K

P

K

Q

N

Q

A

L

T

T

G

G

K

K

R

T

A

A

E

E

A

K

A

V

L

L

G

D

R

E

A

V

Y

G

V

I

T

T

C

V

N

N

K

K

N
|
N

G

T

I

I

P

P

F

Y

D

D

P

P

E

E

K
x
S

P
|
P

F
x
G

V

V

T

T

S

S

G

G

V

I

R
|
R

L

I

G

G

T

T

P

A

A

A

G

V

T

T

T

T

R

R

G

G

F

F

K

G

E

L

A

E

E

E

F

M

K

D

E

E

I

I

G

A

E

A

L

I

I

I

V

-

E

-

V

-

L

-

D

-

G

G

L

L

K

V

A

L

A

K

N

N

S

V

D

G

E

S

G

E

N

Q

A

A

A

L

V

E

E

E

A

A

G

-

V

-

R

R

E

Q

K

R

V

V

M

A

K

A

L

L

T

T

active site: Y51 (= Y61), E53 (= E63), D197 (= D207), T223 (= T233), K226 (= K236), R232 (= R242)
binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E53 (= E63), Y60 (= Y70), Y61 (= Y71), L117 (= L127), G121 (= G131), H122 (= H132), L123 (= L133), S172 (≠ T182), K248 (≠ S259), F251 (= F262), N341 (= N352), S349 (≠ K360), P350 (= P361), G351 (≠ F362), R357 (= R368)
binding glycine: S31 (= S41), Y51 (= Y61), Y61 (= Y71), H200 (= H210), K226 (= K236), R357 (= R368)
binding pyridoxal-5'-phosphate: Y51 (= Y61), S93 (= S103), G94 (= G104), A95 (≠ S105), H122 (= H132), S172 (≠ T182), D197 (= D207), A199 (= A209), H200 (= H210), T223 (= T233), K226 (= K236), G257 (= G268)

2vmxA Crystal structure of f351gbsshmt in complex with l-allo-thr (see paper)
62% identity, 95% coverage: 12:422/431 of query aligns to 2:404/405 of 2vmxA

query
sites
2vmxA

R

K

S

Y

L

L

A

P

D

Q

S

Q

D

D

P

P

D

Q

I

V

F

F

G

A

A

A

I

I

E

E

K

Q

E

E

L

R

G

K

R

R

Q

Q

R

H

H

A

E

K

I

I

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

V

S

S

R

R

A

A

V

V

L

M

E

E

A

A

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

Y

P

P

G

G

K

R

R

R

Y

Y

Y

Y

G

G

G

G

C

C

Q

E

Y

Y

V

V

D

D

I

I

A

V

E

E

E

E

L

L

A

A

I

R

E

E

R

R

A

A

K

K

K

Q

L

L

F

F

G

G

V

A

G

E

F

H

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

M

A

N

N

Q

M

A

A

V

V

F

Y

L

F

A

T

L

V

L

L

Q

E

P

H

G

G

D

D

T

T

F

V

M

L

G

G

L

M

D

N

L

L

N

S

S

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

V

N

N

M

F

S

S

G

G

K

V

W

Q

F

Y

N

N

V

F

V

V

S

A

Y

Y

G

G

V

V

R

D

E

P

D

E

D

T

H

H

L

V

L

I

D

D

M

Y

D

D

D

D

V

V

A

R

K

E

K

K

A

A

R

R

E

L

H

H

K

R

P

P

K

K

L

L

I

I

I

V

A

A

G

A

G

A

T
x
S

A

A

Y

Y

S

P

R

R

I

I

W

I

D

D

W

F

K

A

R

K

F

F

R

R

E

E

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

M

M

V

V

D
|
D

M

M

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

G

A

D

G

Q

L

H

H

P

P

S

N

P

P

F

V

P

P

H

Y

C

A

H

H

V

F

A

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

M

M

I

I

L

L

T

C

N

Q

D

-

E

E

E

Q

I

F

A

A

K

K

K

Q

I

I

N

D

S

K

A

A

V

I

F

F

P

P

G

G

L

I

Q

Q

G

G

G

G

P

P

L

L

M

M

H

H

V

V

I

I

A

A

A

A

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

Q

Q

P

D

S

D

F

F

K

K

D

A

Y

Y

A

A

A

K

Q

R

V

V

V

V

K

D

N

N

A

A

R

K

T

R

L

L

A

A

D

S

T

A

L

L

K

Q

A

N

N

E

G

G

L

F

D

T

I

L

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

L

L

L

V

V

D

D

L

L

R

R

K

P

K

Q

N

Q

A

L

T

T

G

G

K

K

R

T

A

A

E

E

A

K

A

V

L

L

G

D

R

E

A

V

Y

G

V

I

T

T

C

V

N

N

K

K

N

N

G

T

I

I

P

P

F

Y

D

D

P

P

E

E

K

S

P

P

F

G

V

V

T

T

S

S

G

G

V

I

R
|
R

L

I

G

G

T

T

P

A

A

A

G

V

T

T

T

T

R

R

G

G

F

F

K

G

E

L

A

E

E

E

F

M

K

D

E

E

I

I

G

A

E

A

L

I

I

I

V

-

E

-

V

-

L

-

D

-

G

G

L

L

K

V

A

L

A

K

N

N

S

V

D

G

E

S

G

E

N

Q

A

A

A

L

V

E

E

E

A

A

G

-

V

-

R

R

E

Q

K

R

V

V

M

A

K

A

L

L

T

T

active site: Y51 (= Y61), E53 (= E63), D197 (= D207), T223 (= T233), K226 (= K236), R232 (= R242)
binding allo-threonine: S31 (= S41), H122 (= H132), H200 (= H210), R357 (= R368)
binding pyridoxal-5'-phosphate: S93 (= S103), G94 (= G104), A95 (≠ S105), H122 (= H132), S172 (≠ T182), D197 (= D207), A199 (= A209), H200 (= H210), T223 (= T233), K226 (= K236)

6ymfA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the plp-serine external aldimine state (see paper)
58% identity, 97% coverage: 14:431/431 of query aligns to 7:417/418 of 6ymfA

query
sites
6ymfA

L

L

A

L

D

Q

S

T

D

D

P

P

D

T

I

I

F

S

G

G

A

M

I

M

E

Q

K

K

E

E

L

L

G

Q

R

R

Q

Q

R

R

H

E

E

H

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

T

S

S

R

P

A

A

V

V

L

M

E

A

A

T

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

L

P

P

G

K

K

K

R

R

Y

Y

Y

Y

G

G

G

G

C

C

Q

E

Y

F

V

I

D

D

I

E

A

I

E

E

E

Q

L

V

A

A

I

I

E

D

R

R

A

A

K

K

K

E

L

L

F

F

G

G

V

A

G

A

F

S

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

M

A

N

N

Q

F

A

A

V

V

F

F

L

L

A

T

L

L

L

L

Q

K

P

P

G

G

D

D

T

K

F

I

M

M

G

G

L

M

D

D

L

L

N

S

S

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

A

N

N

M

V

S

S

G

G

K

K

W

W

F

F

N

E

V

A

V

V

S

H

Y

Y

G

G

V

V

R

S

E

Q

D

E

D

T

H

E

L

Q

L

L

D

D

M

Y

D

D

D

H

V

I

A

L

K

E

K

V

A

A

R

R

E

Q

H

E

K

R

P

P

K

K

L

L

I

I

I

I

A

C

G

G

G

Y

T
x
S

A

A

Y

Y

S

P

R

R

I

I

W

I

D

N

W

F

K

E

R

K

F

F

R

R

E

A

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

M

L

V

A

D
|
D

M

I

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V

A

A

G

S

D

G

Q

H

H

H

P

P

S

N

P

P

F

V

P

P

H

H

C

C

H

D

V

V

A

V

T

T

T

T

T
|
T

T

T

H

H

K
|
K

S

T

L

L

R

R

G

G

P

P

R
|
R

G

G

G

G

M

L

I

I

L

L

T

T

N

R

D

D

E

P

E

E

I

L

A

G

K

K

K

K

I

F

N

N

S

K

A

S

V

V

F

F

P

P

G

G

L

T

Q

Q

G

G

G

G

P

P

L

L

M

E

H

H

V

V

I

I

A

A

A

G

K

K

A

A

V

V

A

A

F

F

G

G

E

E

A

A

L

L

Q

K

P

P

S

E

F

F

K

K

D

A

Y

Y

A

S

A

G

Q

Q

V

V

V

I

K

A

N

N

A

A

R

Q

T

A

L

M

A

A

D

Q

T

Q

L

L

K

Q

A

N

N

R

G

G

L

F

D

K

I

I

V

V

S

S

G

G

G

G

T

T

D

D

N

N

H

H

L

L

M

M

L

L

V

V

D

D

L

L

R

R

K

S

K

V

N

N

A

L

T

T

G

G

K

K

R

Q

A

A

E

D

A

Q

A

L

L

V

G

S

R

D

A

V

Y

N

V

I

T

T

C

A

N

N

K

K

N

N

G

T

I

V

P

P

F

F

D

D

P

P

E

E

K

S

P

P

F

F

V

V

T

T

S

S

G

G

V

L

R
|
R

L

L

G

G

T

S

P

P

A

A

G

M

T

T

T

T

R

R

G

G

F

L

K

G

E

T

A

E

E

D

F

F

K

A

E

E

I

I

G

A

E

N

L

I

I

I

V

A

E

D

V

R

L

L

D

-

G

-

L

-

K

-

A

-

A

-

N

-

S

Q

D

N

E

P

G

E

N

D

A

E

A

Q

V

V

E

K

A

Q

G

A

V

C

R

V

E

Q

K

R

V

V

M

A

K

A

L

L

T

C

G

E

R

R

F

F

P

P

M

L

Y

Y

G

P

Y

H

M

L

active site: Y54 (= Y61), E56 (= E63), D200 (= D207), T226 (= T233), K229 (= K236), R235 (= R242)
binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S34 (= S41), S96 (= S103), G97 (= G104), A98 (≠ S105), H125 (= H132), S175 (≠ T182), D200 (= D207), A202 (= A209), H203 (= H210), T226 (= T233), K229 (= K236), R361 (= R368)

6ymdA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the covalent complex with malonate (see paper)
58% identity, 97% coverage: 14:431/431 of query aligns to 7:417/420 of 6ymdA

query
sites
6ymdA

L

L

A

L

D

Q

S

T

D

D

P

P

D

T

I

I

F

S

G

G

A

M

I

M

E

Q

K

K

E

E

L

L

G

Q

R

R

Q

Q

R

R

H

E

E

H

I

L

E

E

L

L

I

I

A

A

S
|
S

E

E

N

N

I

F

V

T

S

S

R

P

A

A

V

V

L

M

E

A

A

T

Q

Q

G

G

S

S

I

V

M

L

T

T

N

N

K

K

Y
|
Y

A

A

E
|
E

G

G

Y

L

P

P

G

K

K

K

R

R

Y

Y

Y
|
Y

G

G

G

G

C

C

Q

E

Y

F

V

I

D

D

I

E

A

I

E

E

E

Q

L

V

A

A

I

I

E

D

R

R

A

A

K

K

K

E

L

L

F

F

G

G

V

A

G

A

F

S

A

A

N

N

V

V

Q

Q

P

P

N

H

S
|
S

G
|
G

S
x
A

Q

Q

M

A

N

N

Q

F

A

A

V

V

F

F

L

L

A

T

L

L

L

L

Q

K

P

P

G

G

D

D

T

K

F

I

M

M

G

G

L

M

D

D

L

L

N

S

S

H

G

G

G

G

H
|
H

L

L

T

T

H

H

G

G

S

S

P

P

V

A

N

N

M

V

S

S

G

G

K

K

W

W

F

F

N

E

V

A

V

V

S

H

Y

Y

G

G

V

V

R

S

E

Q

D

E

D

T

H

E

L

Q

L

L

D

D

M

Y

D

D

D

H

V

I

A

L

K

E

K

V

A

A

R

R

E

Q

H

E

K

R

P

P

K

K

L

L

I

I

I

I

A

C

G

G

G
x
Y

T
x
S

A

A

Y

Y

S

P

R

R

I

I

W

I

D

N

W

F

K

E

R

K

F

F

R

R

E

A

I

I

A

A

D

D

E

E

V

V

G

G

A

A

W

Y

L

L

M

L

V

A

D
|
D

M

I

A
|
A

H
|
H

I

I

A

A

G

G

L

L

V

V<