SitesBLAST

P78 (= P5) to R: in CBSD; severe form; associated in cis with N-102; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204608
G85 (= G12) to R: in CBSD; loss of cystathionine beta-synthase activity; dbSNP:rs863223435
T87 (= T14) to N: in CBSD; decreased cystathionine beta-synthase activity; increased aggregation
L101 (vs. gap) to P: in CBSD; common mutation in Irish population; loss of activity; dbSNP:rs786204757
K102 (vs. gap) to N: in CBSD; associated in cis with R-78; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs786204609; to Q: in dbSNP:rs34040148
C109 (≠ A34) to R: in CBSD; loss of activity; dbSNP:rs778220779
A114 (≠ P39) to V: in CBSD; mild form; when linked with W-58 severe form; decreased cystathionine beta-synthase activity; decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs121964964
K119 (= K44) modified: N6-(pyridoxal phosphate)lysine
R125 (≠ W50) to Q: in CBSD; severe form; when linked with D-131 moderate form; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs781444670; to W: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs886057100
M126 (≠ I51) to V: in CBSD; loss of activity
E131 (= E56) to D: in CBSD; linked with Q-125; loss of activity; dbSNP:rs1555875351
G139 (= G64) to R: in CBSD; mild form; dbSNP:rs121964965
I143 (≠ V68) to M: in CBSD; 4% of activity; stable; dbSNP:rs370167302
E144 (= E69) to K: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964966
G148 (= G73) to R: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs755952006
N149 (= N74) binding pyridoxal 5'-phosphate
L154 (= L79) to Q: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
A155 (= A80) to T: in CBSD; complete loss of activity; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs1429138569; to V: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity
C165 (≠ T90) to Y: in CBSD; severe form; protein expression is comparable to wild-type; loss of cystathionine beta-synthase activity; no effect on homotetramer formation; dbSNP:rs1347651454
M173 (≠ Q98) to V: in CBSD; presents 40% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; natural variant: Missing (in CBSD; loss of activity)
E176 (= E101) to K: in CBSD; severe form; loss of cystathionine beta-synthase activity; inhibited by AdoMet; severely decreases homotetramer formation by promoting formation of larger aggregates; dbSNP:rs762065361
V180 (≠ A105) to A: in CBSD; decreased cystathionine beta-synthase activity; decreases homotetramer formation; dbSNP:rs1555875010
T191 (≠ V116) to M: in CBSD; moderate and severe forms; loss of cystathionine beta-synthase activity; absent capacity to form multimeric quaternary structure; dbSNP:rs121964973
K211 (≠ A135) modified: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)
A226 (≠ V155) to T: in CBSD; presents 20% of the wild-type activity; dramatically reduced capacity to form multimeric quaternary structure; dbSNP:rs763835246
N228 (= N157) to K: in CBSD; loss of cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs1464223176; to S: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1555874803
A231 (= A160) to P: in CBSD; has significantly decreased levels of enzyme activity
D234 (= D163) to N: in CBSD; decreased cystathionine beta-synthase activity; changed localization; decreased interaction with pyridoxal 5'-phosphate; no effect on homotetramer formation; dbSNP:rs773734233; natural variant: Missing (in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity)
GTGGT 256:260 (≠ GSGGT 185:189) binding pyridoxal 5'-phosphate
T257 (≠ S186) to M: in CBSD; moderate to severe form; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs758236584
T262 (≠ A191) to M: in CBSD; moderate form; dbSNP:rs149119723; to R: in CBSD; severe form; loss of cystathionine beta-synthase activity; loss of homotetramer formation
R266 (≠ E195) to K: in CBSD; mild form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; no effect on heme-binding; decreased stability; dbSNP:rs121964969
K269 (≠ R198) natural variant: Missing (in CBSD; loss of expression)
C272 (≠ K201) mutation to A: Reduced heme content and cystathionine beta-synthase activity.
C275 (≠ I204) to Y: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; mutation to S: Reduced heme content and cystathionine beta-synthase activity.
I278 (≠ G207) to S: in CBSD; loss of activity; to T: in CBSD; mild to severe form; common mutation; decreased expression; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; severely affects homotetramer formation by promoting formation of larger aggregates; dbSNP:rs5742905
D281 (= D210) to N: in CBSD; loss of activity
A288 (≠ Y217) to T: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs141502207
E302 (≠ S231) to K: in CBSD; no effect on cystathionine beta-synthase activity; inhibited by AdoHcy and impaired activation by AdoMet; no effect on homotetramer formation; dbSNP:rs779270933
G305 (= G235) to R: in CBSD; loss of cystathionine beta-synthase activity; no effect on homotetramer formation
G307 (= G237) to S: in CBSD; moderate to severe form; linked with D-534; common mutation; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; no effect on homotetramer formation; dbSNP:rs121964962
V320 (≠ P250) to A: in CBSD; has 36% of wild-type enzyme activity; dbSNP:rs781567152
D321 (= D251) to V: in CBSD; loss of activity
R336 (≠ F266) to C: in CBSD; protein expression is comparable to wild-type; loss of activity; absent capacity to form multimeric quaternary structure; dbSNP:rs398123151; to H: in CBSD; mild form; no effect on expression; exhibits an activity lower than 4% of the wild-type enzyme; altered stimulation by AdoMet; absent capacity to form multimeric quaternary structure; dbSNP:rs760417941
L338 (= L268) to P: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
G347 (= G277) to S: in CBSD; protein expression is comparable to wild-type; loss of activity; dbSNP:rs771298943
S349 (= S279) binding pyridoxal 5'-phosphate; to N: in CBSD; severe form; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
T353 (≠ N283) to M: in CBSD; protein expression is comparable to wild-type; significant decrease of enzyme activity; dbSNP:rs121964972
R369 (≠ T299) to C: in CBSD; when linked with C-491 severe form; decreased cystathionine beta-synthase activity; decreased homotetramer formation; dbSNP:rs117687681
D376 (= D306) to N: in CBSD; has significantly decreased levels of enzyme activity; dbSNP:rs1170128038
R379 (≠ N309) to Q: in CBSD; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure; dbSNP:rs763036586
K384 (= K314) to E: in CBSD; severe form; dbSNP:rs121964967

Sites not aligning to the query:

18 R → C: results in 1/3 to 2/3 the enzyme activity of the wild-type; dbSNP:rs201827340
49 P → L: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs148865119
52 binding axial binding residue
58 R → W: in CBSD; linked with V-114; 18% of activity; dbSNP:rs555959266
65 binding axial binding residue; H → R: in CBSD; decreased cystathionine beta-synthase activity; inhibited by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs1191141364
69 A → P: in dbSNP:rs17849313
422 P → L: in CBSD; changed cystathionine beta-synthase activity; impaired stimulation by AdoMet; does not affect homotetramer formation; dbSNP:rs28934892
427 P → L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs863223434
435 I → T: in CBSD; no effect on cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; does not affect homotetramer formation
439 R → Q: in CBSD; no effect on cystathionine beta-synthase activity; increased homotetramer formation; dbSNP:rs756467921
444 D → N: in CBSD; decreased expression; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; increased homotetramer formation; dbSNP:rs28934891
449 V → G: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
456 L → P: in CBSD; severe; exhibits an activity lower than 4% of the wild-type enzyme; absent capacity to form multimeric quaternary structure
466 S → L: in CBSD; increased cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; decreased homotetramer formation; dbSNP:rs121964971
500 S → L: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet; dbSNP:rs755106884
526 Q → K: in CBSD; has significantly decreased levels of enzyme activity
539 L → S: in CBSD; loss of cystathionine beta-synthase activity; impaired stimulation by AdoMet and AdoHcy; loss of homotetramer formation; dbSNP:rs121964968
540 L → Q: in CBSD; no effect on cystathionine beta-synthase activity; altered stimulation by AdoMet
548 R → Q: presents 60% of the wild-type activity; highly reduced capacity to form multimeric quaternary structure; dbSNP:rs150828989

8s5hA Full-length human cystathionine beta-synthase with c-terminal 6xhis- tag, basal state, helical reconstruction (see paper)
40% identity, 94% coverage: 3:326/346 of query aligns to 35:355/507 of 8s5hA

query
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binding protoporphyrin ix containing fe: A185 (≠ V155), Y192 (≠ I162), R225 (≠ E195)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 17, 22, 23, 24

7qgtB Crystal structure of human cystathionine beta-synthase (delta516-525) in complex with aoaa. (see paper)
40% identity, 94% coverage: 3:326/346 of query aligns to 36:356/500 of 7qgtB

query
sites
7qgtB

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binding protoporphyrin ix containing fe: A186 (≠ V155), P189 (≠ R158), L190 (≠ Q159), Y193 (≠ I162), R226 (≠ E195)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: K79 (= K44), T106 (= T71), S107 (≠ A72), N109 (= N74), T110 (= T75), Q182 (= Q151), G216 (= G185), T217 (≠ S186), G218 (= G187), T220 (= T189), G265 (= G235), S309 (= S279), P335 (≠ C305), D336 (= D306)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 10, 11, 12, 13, 14, 23, 24, 25

1jbqA Structure of human cystathionine beta-synthase: a unique pyridoxal 5'- phosphate dependent hemeprotein (see paper)
40% identity, 94% coverage: 3:326/346 of query aligns to 34:347/348 of 1jbqA

query
sites
1jbqA

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V

T

P

P

A

T

V

-

P

-

Y

-

R

-

N

-

P

-

N

E

N

S

Y

H

V

V

K

G

I

V

S

A

G

W

R

R

L

L

A

K

A

N

R

E

L

I

A

-

E

-

T

-

E

-

P

P

N

N

G

S

A

H

I

I

W

-

A

L

N

D

Q

Q

F

Y

D

R

N

N

V
x
A

A

S

N

N

R
x
P

Q
x
L

A

A

H

H

I
x
Y

D

D

T

T

A

A

P

D

E

E

I

I

W

L

R

Q

D

Q

T

C

D

D

G

G

K

K

V

L

D

D

G

M

F

L

I

V

C

A

A

S

V
|
V

G
|
G

S
x
T

G
|
G

T
|
T

L

I

A

T

G

G

V

I

A

A

E
x
R

G

K

L

L

R

K

A

E

R

K

K

C

A

P

G

G

I

C

K

R

I

I

G

I

I

G

A

V

D
|
D

P

P

E

E

G

G

A
x
S

A

I

L
|
L

Y

A

N

E

F

P

Y

E

A

E

H

L

G

N

E

Q

L

T

K

E

S

Q

S

T

G

T

S

Y

S

E

I

V

T

-

E

E

G
|
G

I

I

G

G

Q

Y

G

D

R

F

I

I

T

P

A

T

N

V

L

L

E

D

G

R

F

T

T

V

P

V

D

D

F

K

A

W

Y

F

Q

K

I

S

P

N

D

D

A

E

E

E

A

A

V

F

P

T

Y

F

V

A

F

R

E

M

L

L

I

I

E

A

K

Q

E

E

G

G

I

L

C

L

I

C

G

G

S
|
S

T

A

G

G

I

S

N

T

V

V

A

A

G

V

A

A

V

V

R

K

L

A

A

A

R

Q

E

E

L

L

G

Q

P

E

G

G

H

Q

T

R

I

C

V

V

T

V

I

I

L

L

C
x
P

D
|
D

Y

S

G

V

N

R

R

N

Y

Y

Q

M

S

T

K

K

L

F

F

L

N

S

P

D

D

R

F

W

L

M

A

L

S

Q

K

K

G

G

L

F

active site: K77 (= K44), S105 (≠ A72), D232 (= D210), S236 (≠ A214), L238 (= L216), S300 (= S279), P326 (≠ C305)
binding protoporphyrin ix containing fe: A177 (≠ V155), P180 (≠ R158), L181 (≠ Q159), Y184 (≠ I162), R217 (≠ E195)
binding pyridoxal-5'-phosphate: K77 (= K44), N107 (= N74), V206 (= V184), G207 (= G185), T208 (≠ S186), G209 (= G187), G210 (= G188), T211 (= T189), G256 (= G235), S300 (= S279), P326 (≠ C305), D327 (= D306)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 9, 10, 11, 12, 16, 20, 21, 22, 23

P9WP51 Probable cystathionine beta-synthase Rv1077; Beta-thionase; Serine sulfhydrase; EC 4.2.1.22 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
39% identity, 94% coverage: 1:326/346 of query aligns to 1:316/464 of P9WP51

query
sites
P9WP51

M

M

P

R

V

I

L

A

P

Q

S

H

V

I

L

S

E

E

A

L

I

I

G

G

N

G

T

T

P

P

L

L

I

V

R

R

L

L

K

N

A

S

V

V

S

V

E

P

A

D

T

G

G

A

C

G

N

T

I

V

L

A

G

A

K

K

A

V

E

E

F

Y

L

L

N

N

P

P

G

G

Q

G

S

S

V

S

K

K

D

D

R

R

A

I

A

A

L

V

W

K

I

M

I

I

R

E

Q

A

A

A

E

E

K

A

A

S

G

G

Q

Q

L

L

R

K

P

P

G

G

V

T

I

I

V

V

E

E

G

P

T

T

A

S

G

G

N

N

T

T

G

G

I

V

G

G

L

L

A

A

V

L

V

V

A

A

S

Q

A

R

L

R

G

G

Y

Y

R

K

T

C

V

V

I

F

V

V

I

C

P

P

E

D

T

K

Q

V

S

S

Q

E

E

D

K

K

K

R

D

N

A

V

L

L

R

I

L

A

L

Y

G

G

A

A

E

E

L

V

V

V

E

V

V

C

P

P

-

T

A

A

V

V

P

P

Y

P

R

H

N

D

P

P

N

A

N

S

Y

Y

V

Y

K

S

I

V

S

S

G

D

R

R

L

L

A

V

A

R

R

D

L

I

A

-

E

-

T

-

E

-

P

-

N

D

G

G

A

A

I

W

W

K

A

P

N

D

Q

Q

F

Y

D

A

N

N

V

P

A

E

N

G

R

P

Q

A

A

S

H

H

I

Y

D

V

T

T

A

G

P

P

E

E

I

I

W

W

R

A

D

D

T

T

D

E

G

G

K

K

V

V

D

T

G

H

F

F

I

V

C

A

A

G

V

I

G

G

S

T

G

G

T

T

L

I

A

T

G

G

V

A

A

G

E

R

G

Y

L

L

R

K

A

E

R

V

K

S

A

G

G

G

-

R

I

V

K

R

I

I

G

V

I

G

A

A

D

D

P

P

E

E

G

G

A

-

L

-

Y

-

N

S

F

V

Y

Y

A

S

H

G

G

G

E

A

L

G

K

R

S

-

G

-

S

P

S

Y

I

L

T

V

E

E

G

G

I

V

G

G

Q

E

G

D

R

F

I

W

T

P

A

A

N

A

L

Y

E

D

G

P

F

S

T

V

P

P

D

D

F

E

A

I

Y

I

Q

A

I

V

P

S

D

D

A

S

E

D

A

S

V

F

P

D

Y

M

V

T

F

R

E

R

L

L

I

A

E

R

K

E

E

E

G

A

I

M

C

L

I

V

G

G

S

S

T

C

G

G

I

M

N

A

V

V

A

V

G

A

A

A

V

L

R

K

L

V

A

A

R

E

E

E

L

A

G

G

P

P

G

D

H

A

T

L

I

I

V

V

T

V

I

L

L

L

C

P

D

D

Y

G

G

G

N

R

R

G

Y

Y

Q

M

S

S

K

K

L

I

F

F

N

N

P

D

D

A

F

W

L

M

A

S

S

S

K

Y

G

G

L

F

Sites not aligning to the query:

428 modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

7xoyA Cystathionine beta-synthase of mycobacterium tuberculosis in the presence of s-adenosylmethionine and serine. (see paper)
39% identity, 92% coverage: 7:326/346 of query aligns to 5:314/458 of 7xoyA

query
sites
7xoyA

V

I

L

S

E

E

A

L

I

I

G

G

N

G

T

T

P

P

L

L

I

V

R

R

L

L

K

N

A

S

V

V

S

V

E

P

A

D

T

G

G

A

C

G

N

T

I

V

L

A

G

A

K

K

A

V

E

E

F

Y

L

L

N

N

P

P

G

G

Q

G

S

S

V

S

K
|
K

D

D

R

R

A

I

A

A

L

V

W

K

I

M

I

I

R

E

Q

A

A

A

E

E

K

A

A

S

G

G

Q

Q

L

L

R

K

P

P

G

G

V

T

I

I

V

V

E

E

G

P

T

T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

V

G

G

L

L

A

A

V

L

V

V

A

A

S

Q

A

R

L

R

G

G

Y

Y

R

K

T

C

V

V

I

F

V

V

I

C

P

P

E

D

T

K

Q

V

S

S

Q

E

E

D

K

K

K

R

D

N

A

V

L

L

R

I

L

A

L

Y

G

G

A

A

E

E

L

V

V

V

E

V

V

C

P

P

-

T

A

A

V

V

P

P

Y

P

R

H

N

D

P

P

N

A

N

S

Y

Y

V

Y

K

S

I

V

S

S

G

D

R

R

L

L

A

V

A

R

R

D

L

I

A

-

E

-

T

-

E

-

P

-

N

D

G

G

A

A

I

W

W

K

A

P

N

D

Q

Q

F

Y

D

A

N

N

V

P

A

E

N

G

R

P

Q

A

A

S

H

H

I

Y

D

V

T

T

A

G

P

P

E

E

I

I

W

W

R

A

D

D

T

T

D

E

G

G

K

K

V

V

D

T

G

H

F

F

I

V

C

A

A

G

V

I

G

G

S
x
T

G

G

G
|
G

T
|
T

L

I

A

T

G

G

V

A

A

G

E

R

G

Y

L

L

R

K

A

E

R

V

K

S

A

G

G

G

-

R

I

V

K

R

I

I

G

V

I

G

A

A

D

D

P

P

E

E

G

G

A

-

L

-

Y

-

N

S

F

V

Y

Y

A

S

H

G

G

G

E

A

L

G

K

R

S

-

G

-

S

P

S

Y

I

L

T

V

E

E

G

G

I

V

G

G

Q

E

G

D

R

F

I

W

T

P

A

A

N

A

L

Y

E

D

G

P

F

S

T

V

P

P

D

D

F

E

A

I

Y

I

Q

A

I

V

P

S

D

D

A

S

E

D

A

S

V

F

P

D

Y

M

V

T

F

R

E

R

L

L

I

A

E

R

K

E

E

E

G

A

I

M

C

L

I

V

G

G

S
|
S

T

C

G

G

I

M

N

A

V

V

A

V

G

A

A

A

V

L

R

K

L

V

A

A

R

E

E

E

L

A

G

G

P

P

G

D

H

A

T

L

I

I

V

V

T

V

I

L

L

L

C
x
P

D

D

Y

G

G

G

N

R

R

G

Y

Y

Q

M

S

S

K

K

L

I

F

F

N

N

P

D

D

A

F

W

L

M

A

S

S

S

K

Y

G

G

L

F

binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: K42 (= K44), S70 (≠ A72), N72 (= N74), T73 (= T75), T180 (≠ S186), G182 (= G188), T183 (= T189), S267 (= S279), P293 (≠ C305)

7xnzB Native cystathionine beta-synthase of mycobacterium tuberculosis. (see paper)
39% identity, 92% coverage: 7:326/346 of query aligns to 5:314/458 of 7xnzB

query
sites
7xnzB

V

I

L

S

E

E

A

L

I

I

G

G

N

G

T

T

P

P

L

L

I

V

R

R

L

L

K

N

A

S

V

V

S

V

E

P

A

D

T

G

G

A

C

G

N

T

I

V

L

A

G

A

K

K

A

V

E

E

F

Y

L

L

N

N

P

P

G

G

Q

G

S

S

V

S

K
|
K

D

D

R

R

A

I

A

A

L

V

W

K

I

M

I

I

R

E

Q

A

A

A

E

E

K

A

A

S

G

G

Q

Q

L

L

R

K

P

P

G

G

V

T

I

I

V

V

E

E

G

P

T

T

A

S

G

G

N

N

T

T

G

G

I

V

G

G

L

L

A

A

V

L

V

V

A

A

S

Q

A

R

L

R

G

G

Y

Y

R

K

T

C

V

V

I

F

V

V

I

C

P

P

E

D

T

K

Q

V

S

S

Q

E

E

D

K

K

K

R

D

N

A

V

L

L

R

I

L

A

L

Y

G

G

A

A

E

E

L

V

V

V

E

V

V

C

P

P

-

T

A

A

V

V

P

P

Y

P

R

H

N

D

P

P

N

A

N

S

Y

Y

V

Y

K

S

I

V

S

S

G

D

R

R

L

L

A

V

A

R

R

D

L

I

A

-

E

-

T

-

E

-

P

-

N

D

G

G

A

A

I

W

W

K

A

P

N

D

Q

Q

F

Y

D

A

N

N

V

P

A

E

N

G

R

P

Q

A

A

S

H

H

I

Y

D

V

T

T

A

G

P

P

E

E

I

I

W

W

R

A

D

D

T

T

D

E

G

G

K

K

V

V

D

T

G

H

F

F

I

V

C

A

A

G

V

I

G
|
G

S
x
T

G
|
G

T
|
T

L

I

A

T

G

G

V

A

A

G

E

R

G

Y

L

L

R

K

A

E

R

V

K

S

A

G

G

G

-

R

I

V

K

R

I

I

G

V

I

G

A

A

D

D

P

P

E

E

G

G

A

-

L

-

Y

-

N

S

F

V

Y

Y

A

S

H

G

G

G

E

A

L

G

K

R

S

-

G

-

S

P

S

Y

I

L

T

V

E

E

G

G

I

V

G

G

Q

E

G

D

R

F

I

W

T

P

A

A

N

A

L

Y

E

D

G

P

F

S

T

V

P

P

D

D

F

E

A

I

Y

I

Q

A

I

V

P

S

D

D

A

S

E

D

A

S

V

F

P

D

Y

M

V

T

F

R

E

R

L

L

I

A

E

R

K

E

E

E

G

A

I

M

C

L

I

V

G

G

S
|
S

T

C

G

G

I

M

N

A

V

V

A

V

G

A

A

A

V

L

R

K

L

V

A

A

R

E

E

E

L

A

G

G

P

P

G

D

H

A

T

L

I

I

V

V

T

V

I

L

L

L

C
x
P

D
|
D

Y

G

G

G

N

R

R

G

Y

Y

Q

M

S

S

K

K

L

I

F

F

N

N

P

D

D

A

F

W

L

M

A

S

S

S

K

Y

G

G

L

F

binding pyridoxal-5'-phosphate: K42 (= K44), G179 (= G185), T180 (≠ S186), G181 (= G187), G182 (= G188), T183 (= T189), S267 (= S279), P293 (≠ C305), D294 (= D306)

6xwlC Cystathionine beta-synthase from toxoplasma gondii (see paper)
35% identity, 94% coverage: 3:326/346 of query aligns to 8:329/477 of 6xwlC

query
sites
6xwlC

V

I

L

L

P

D

S

S

V

V

L

L

E

D

A

A

I

I

G

G

N

N

T

T

P

P

L

M

I

V

R

R

L

M

K

K

A

R

V

L

S

A

E

K

A

V

T

Y

G

G

-

L

-

E

C

C

N

D

I

L

L

L

G

A

K

K

A

C

E

E

F

F

L

M

N

S

P

A

G

G

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

K

W

A

I

M

I

V

R

E

Q

K

A

A

E

E

K

R

A

E

G

G

Q

R

L

L

R

K

P

A

G

G

G

D

V

T

I

L

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

V

L

V

A

A

A

S

A

A

V

L

R

G

G

Y

Y

R

R

T

M

V

I

I

V

V

T

I

M

P

P

E

A

T

K

Q

M

S

S

Q

A

E

E

K

K

K

S

D

N

A

I

L

M

R

K

L

C

L

L

G

G

A

A

E

E

L

I

V

V

E

R

V

T

P

P

A

T

-

E

V

A

P

A

Y

W

R

N

N

D

P

E

N

N

N

S

Y

H

V

M

K

G

I

V

S

A

G

A

R

K

L

L

A

-

R

-

L

-

A

-

E

Q

T

R

E

E

P

L

N

E

G

N

A

A

I

H

W

I

A

L

N

D

Q

Q

F

Y

D

N

N

N

V

T

A

A

N

N

R

P

Q

M

A

V

H

H

I

Y

D

D

T

V

T

T

A

A

P

E

E

E

I

I

W

I

R

T

D

Q

T

C

D

D

G

G

K

D

V

I

D

D

G

M

F

V

I

V

C

I

A

G

V

A

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

I

A

T

G

G

V

I

A

G

E

R

G

K

L

I

R

K

A

E

R

R

K

C

A

P

G

K

I

C

K

K

I

V

G

V

I

G

A

V

D

D

P

P

E

K

G

G

A

S

A

I

L

L

Y

A

N

V

F

P

Y

D

A

S

H

L

G

N

E

D

L

E

K

K

S

R

S

L

G

Q

S

S

S

Y

I

E

T

V

E

E

G
|
G

I

I

G

G

Q

Y

G

D

R

F

I

V

T

P

A

G

N

V

L

L

E

D

G

R

F

K

T

V

P

V

D

D

F

E

A

W

Y

V

Q

K

I

V

P

G

D

D

A

A

E

E

A

S

V

F

P

T

Y

T

V

A

F

R

E

A

L

I

I

I

E

R

K

N

E

E

G

G

I

L

C

F

I

V

G

G

S
|
S

T

S

G

G

I

A

N

N

V

V

A

W

G

G

A

A

V

L

R

Q

L

A

A

A

R

R

E

Q

L

L

G

K

P

K

G

G

H

Q

T

K

I

C

V

V

T

V

I

L

L

L

C
x
P

D
|
D

Y

S

G

S

N

R

R

N

Y

Y

Q

M

S

S

K

K

L

F

F

I

N

S

P

D

D

E

F

W

L

M

A

A

S

E

K

H

G

G

L

F

binding pyridoxal-5'-phosphate: K51 (= K44), N81 (= N74), G188 (= G185), T189 (≠ S186), G190 (= G187), T192 (= T189), G238 (= G235), S282 (= S279), P308 (≠ C305), D309 (= D306)

6xylA Crystal structure of delta466-491 cystathionine beta-synthase from toxoplasma gondii with l-serine (see paper)
35% identity, 94% coverage: 3:326/346 of query aligns to 8:329/468 of 6xylA

query
sites
6xylA

V

I

L

L

P

D

S

S

V

V

L

L

E

D

A

A

I

I

G

G

N

N

T

T

P

P

L

M

I

V

R

R

L

M

K

K

A

R

V

L

S

A

E

K

A

V

T

Y

G

G

-

L

-

E

C

C

N

D

I

L

L

L

G

A

K

K

A

C

E

E

F

F

L

M

N

S

P

A

G

G

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

K

W

A

I

M

I

V

R

E

Q

K

A

A

E

E

K

R

A

E

G

G

Q

R

L

L

R

K

P

A

G

G

G

D

V

T

I

L

V

I

E

E

G

P

T

T

A

S

G

G

N

N

T
|
T

G

G

I

I

G

G

L

L

A

A

V

L

V

A

A

A

S

A

A

V

L

R

G

G

Y

Y

R

R

T

M

V

I

I

V

V

T

I

M

P

P

E

A

T

K

Q

M

S

S

Q

A

E

E

K

K

K

S

D

N

A

I

L

M

R

K

L

C

L

L

G

G

A

A

E

E

L

I

V

V

E

R

V

T

P

P

A

T

-

E

V

A

P

A

Y

W

R

N

N

D

P

E

N

N

N

S

Y

H

V

M

K

G

I

V

S

A

G

A

R

K

L

L

A

-

R

-

L

-

A

-

E

Q

T

R

E

E

P

L

N

E

G

N

A

A

I

H

W

I

A

L

N

D

Q
|
Q

F

Y

D

N

N

N

V

T

A

A

N

N

R

P

Q

M

A

V

H

H

I

Y

D

D

T

V

T

T

A

A

P

E

E

E

I

I

W

I

R

T

D

Q

T

C

D

D

G

G

K

D

V

I

D

D

G

M

F

V

I

V

C

I

A

G

V

A

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

I

A

T

G

G

V

I

A

G

E

R

G

K

L

I

R

K

A

E

R

R

K

C

A

P

G

K

I

C

K

K

I

V

G

V

I

G

A

V

D

D

P

P

E

K

G

G

A

S

A

I

L

L

Y

A

N

V

F

P

Y

D

A

S

H

L

G

N

E

D

L

E

K

K

S

R

S

L

G

Q

S

S

S

Y

I

E

T

V

E

E

G
|
G

I
|
I

G

G

Q
x
Y

G

D

R

F

I

V

T

P

A

G

N

V

L

L

E

D

G

R

F

K

T

V

P

V

D

D

F

E

A

W

Y

V

Q

K

I

V

P

G

D

D

A

A

E

E

A

S

V

F

P

T

Y

T

V

A

F

R

E

A

L

I

I

I

E

R

K

N

E

E

G

G

I

L

C

F

I

V

G

G

S
|
S

T

S

G

G

I

A

N

N

V

V

A

W

G

G

A

A

V

L

R

Q

L

A

A

A

R

R

E

Q

L

L

G

K

P

K

G

G

H

Q

T

K

I

C

V

V

T

V

I

L

L

L

C
x
P

D
|
D

Y

S

G

S

N

R

R

N

Y

Y

Q

M

S

S

K

K

L

F

F

I

N

S

P

D

D

E

F

W

L

M

A

A

S

E

K

H

G

G

L

F

binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K51 (= K44), T82 (= T75), Q154 (= Q151), G188 (= G185), T189 (≠ S186), G190 (= G187), T192 (= T189), G238 (= G235), I239 (= I236), Y241 (≠ Q238), S282 (= S279), P308 (≠ C305), D309 (= D306)

5ohxA Structure of active cystathionine b-synthase from apis mellifera (see paper)
36% identity, 95% coverage: 3:330/346 of query aligns to 31:351/488 of 5ohxA

query
sites
5ohxA

V

I

L

M

P

P

S

D

V

I

L

L

E

T

A

A

I

I

G

G

N

Q

T

T

P

P

L

L

I

I

R

K

L

L

K

N

A

N

V

I

S

P

E

K

A

S

T

Y

G

G

-

I

-

K

C

C

N

E

I

I

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

A

L

Y

W

R

I

M

I

I

R

Q

Q

D

A

A

E

E

K

D

A

K

G

G

Q

L

L

L

R

K

P

P

G

G

G

C

V

T

I

I

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

V

M

V

A

A

A

S

A

A

V

L

R

G

G

Y

Y

R

K

T

C

V

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

D

E

E

K

K

K

I

D

S

A

T

L

L

R

Y

L

A

L

L

G

G

A

A

E

K

L

I

V

I

E

R

V

T

P

P

A

T

-

E

V

A

P

S

Y

W

R

H

N

S

P

P

N

E

N

A

Y

H

V

I

K

S

I

V

S

A

G

Q

R

K

L

L

A

Q

A

K

R

E

L

I

A

-

E

-

T

-

E

-

P

P

N

N

G

S

A

I

I

I

W

-

A

L

N

D

Q

Q

F

Y

D

T

N

N

V
x
P

A

G

N

N

R
x
P

Q

L

A

A

H

H

I
x
Y

D

D

T

Q

T

T

A

A

P

I

E

E

I

I

W

W

R

K

D

Q

T

C

D

E

G

G

K

K

V

I

D

D

G

Y

F

L

I

V

C

A

A
x
G

V

A

G
|
G

S
x
T

G
|
G

T
|
T

L

I

A

S

G

G

V

I

A

G

E
x
R

G

K

L

L

R

K

A

E

R

L

K

S

A

P

G

N

I

I

K

K

I

I

G

I

I

A

A

V

D

D

P

P

E

K

G

G

A

S

A

I

L

L

Y

-

N

-

F

-

Y

-

A

-

H

D

G

P

E

S

L

S

K

D

S

E

S

V

G

G

S

F

S

Y

I

E

T

V

E

E

G
|
G

I

I

G

G

Q

Y

G

D

R

F

I

I

T

P

A

T

N

V

L

L

E

D

G

R

F

N

T

V

P

I

D

D

F

K

A

W

Y

I

Q

K

I

T

P

E

D

D

A

N

E

E

A

S

V

L

P

N

Y

A

V

A

F

R

E

M

L

L

I

I

E

R

K

Q

E

E

G

G

I

L

C

L

I

C

G

G

S
|
S

T

S

G

G

I

A

N

A

V

L

A

I

G

A

A

A

V

L

R

K

L

I

A

A

R

K

E

D

L

I

G

P

P

E

G

E

H

K

T

R

I

M

V

V

T

I

I

I

L

L

C
x
P

D
|
D

Y

G

G

I

N

R

R

N

Y

Y

Q

L

S

T

K

K

L

F

F

V

N

S

P

E

D

Y

F

W

L

M

A

E

S

T

K

R

G

G

L

F

P

L

V

Q

P

P

A

V

binding protoporphyrin ix containing fe: P181 (≠ V155), P184 (≠ R158), Y188 (≠ I162), R221 (≠ E195)
binding pyridoxal-5'-phosphate: K74 (= K44), N104 (= N74), G209 (≠ A183), G211 (= G185), T212 (≠ S186), G213 (= G187), G214 (= G188), T215 (= T189), G256 (= G235), S300 (= S279), P326 (≠ C305), D327 (= D306)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 4, 7, 8, 9, 10, 14, 16, 17, 18, 19

Q2V0C9 Cystathionine beta-synthase; EC 4.2.1.22 from Apis mellifera (Honeybee) (see paper)
36% identity, 95% coverage: 3:330/346 of query aligns to 35:358/504 of Q2V0C9

query
sites
Q2V0C9

V

I

L

M

P

P

S

D

V

I

L

L

E

T

A

A

I

I

G

G

N

Q

T

T

P

P

L

L

I

I

R

K

L

L

K

N

A

N

V

I

S

P

E

K

A

S

T

Y

G

G

-

I

-

K

C

C

N

E

I

I

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

A

L

Y

W

R

I

M

I

I

R

Q

Q

D

A

A

E

E

K

D

A

K

G

G

Q

L

L

L

R

K

P

P

G

G

G

C

V

T

I

I

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

V

M

V

A

A

A

S

A

A

V

L

R

G

G

Y

Y

R

K

T

C

V

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

D

E

E

K

K

K

I

D

S

A

T

L

L

R

Y

L

A

L

L

G

G

A

A

E

K

L

I

V

I

E

R

V

T

P

P

A

T

-

E

V

A

P

S

Y

W

R

H

N

S

P

P

N

E

N

A

Y

H

V

I

K

S

I

V

S

A

G

Q

R

K

L

L

A

Q

A

K

R

E

L

I

A

-

E

-

T

-

E

-

P

P

N

N

G

S

A

I

I

I

W

-

A

L

N

D

Q

Q

F

Y

D

T

N

N

V

P

A

G

N

N

R

P

Q

L

A

A

H

H

I

Y

D

D

T

Q

T

T

A

A

P

I

E

E

I

I

W

W

R

K

D

Q

T

C

D

E

G

G

K

K

V

I

D

D

G

Y

F

L

I

V

C

A

A

G

V

A

G
|
G

S
x
T

G
|
G

T
|
T

L

I

A

S

G

G

V

I

A

G

E

R

G

K

L

L

R

K

A

E

R

L

K

S

A

P

G

N

I

I

K

K

I

I

G

I

I

A

A

V

D

D

P

P

E

K

G

G

A

S

A

I

L

L

Y

D

N

P

F

-

Y

-

A

S

H

S

G

D

E

S

L

Q

K

N

S

E

S

V

G

G

S

F

S

Y

I

E

T

V

E

E

G

G

I

I

G

G

Q

Y

G

D

R

F

I

I

T

P

A

T

N

V

L

L

E

D

G

R

F

N

T

V

P

I

D

D

F

K

A

W

Y

I

Q

K

I

T

P

E

D

D

A

N

E

E

A

S

V

L

P

N

Y

A

V

A

F

R

E

M

L

L

I

I

E

R

K

Q

E

E

G

G

I

L

C

L

I

C

G

G

S
|
S

T

S

G

G

I

A

N

A

V

L

A

I

G

A

A

A

V

L

R

K

L

I

A

A

R

K

E

D

L

I

G

P

P

E

G

E

H

K

T

R

I

M

V

V

T

I

I

I

L

L

C

P

D

D

Y

G

G

I

N

R

R

N

Y

Y

Q

L

S

T

K

K

L

F

F

V

N

S

P

E

D

Y

F

W

L

M

A

E

S

T

K

R

G

G

L

F

P

L

V

Q

P

P

A

V

K78 (= K44) modified: N6-(pyridoxal phosphate)lysine
N108 (= N74) binding pyridoxal 5'-phosphate
GTGGT 215:219 (≠ GSGGT 185:189) binding pyridoxal 5'-phosphate
S307 (= S279) binding pyridoxal 5'-phosphate

Sites not aligning to the query:

12 binding axial binding residue
23 binding axial binding residue

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
39% identity, 88% coverage: 10:313/346 of query aligns to 6:297/302 of 2efyA

query
sites
2efyA

A

A

I

I

G

G

N

K

T

T

P

P

L

V

I

V

R

R

L

L

K

A

A

K

V

V

S

V

E

E

A

P

T

D

G

M

C

A

N

E

I

V

L

W

G

V

K

K

A

L

E

E

F

G

L

L

N

N

P

P

G

G

Q

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

W

Y

I

M

I

I

R

K

Q

D

A

A

E

E

K

E

A

R

G

G

Q

I

L

L

R

R

P

P

G

G

G

S

-

G

-

Q

V

V

I

I

V

V

E

E

G

P

T
|
T

A
x
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

V

M

V

I

A

A

S

A

A

S

L

R

G

G

Y

Y

R

R

T

L

V

I

I

L

V

T

I

M

P

P

E

A

T

Q

Q

M

S

S

Q

E

E

E

K

R

K

K

D

R

A

V

L

L

R

K

L

A

L

F

G

G

A

A

E

E

L

L

V

V

E

-

V

-

P

-

A

-

V

-

P

-

Y

L

R

T

N

D

P

P

N

E

N

R

Y

R

V

M

K

L

I

A

S

A

G

R

R

E

L

E

A

A

A

L

R

R

L

L

A

K

E

E

T

E

E

-

P

-

N

L

G

G

A

A

I

F

W

M

A

P

N

D

Q
|
Q

F

F

D

K

N

N

V

P

A

A

N

N

R

V

Q

R

A

A

H

H

I

Y

D

E

T

T

A

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

D

E

G

G

K

R

V

I

D

D

G

A

F

F

I

V

C
x
Y

A

G

V

S

G
|
G

S
x
T

G
|
G

G

G

T
|
T

L

I

A

T

G

G

V

V

A

G

E

R

G

Y

L

L

R

K

A

E

R

R

K

I

A

P

G

H

I

V

K

K

I

V

G

I

I

A

A

V

D
x
E

P

P

E

-

G

-

A

-

A

A

L

R

Y
x
S

N

N

F

V

Y

L

A

S

H

G

G

G

E

K

L

M

K

G

S

Q

S

H

G

G

S

-

I

-

T

F

E

Q

G
|
G

I
x
M

G

G

Q
x
P

G

G

R

F

I

I

T

P

A

E

N

N

L

L

E

D

G

L

F

S

T

L

P

L

D

D

F

G

A

V

Y

I

Q

Q

I

V

P

W

D

E

A

E

E

D

A

A

V

F

P

P

Y

L

V

A

F

R

E

R

L

L

I

A

E

R

K

E

E

E

G

G

I

L

C

F

I

L

G

G

G

M

S
|
S

T

S

G

G

I

G

N

I

V

V

A

W

G

A

A

A

V

L

R

Q

L

V

A

A

R

R

E

E

L

L

G

G

P

P

G

G

H

K

T

R

I

V

V

A

T

C

I

I

L

S

C
x
P

D
|
D

Y

G

G

G

N

W

R

K

Y

Y

Q

L

S

S

active site: K40 (= K44), S70 (≠ A72), E200 (≠ D210), S204 (≠ Y217), S263 (= S279)
binding 5-oxohexanoic acid: T69 (= T71), G71 (= G73), T73 (= T75), Q141 (= Q151), G175 (= G185), G219 (= G235), M220 (≠ I236), P222 (≠ Q238)
binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ C182), G175 (= G185), T176 (≠ S186), G177 (= G187), T179 (= T189), G219 (= G235), S263 (= S279), P289 (≠ C305), D290 (= D306)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
39% identity, 88% coverage: 10:313/346 of query aligns to 6:297/302 of 2ecqA

query
sites
2ecqA

A

A

I

I

G

G

N

K

T

T

P

P

L

V

I

V

R

R

L

L

K

A

A

K

V

V

S

V

E

E

A

P

T

D

G

M

C

A

N

E

I

V

L

W

G

V

K

K

A

L

E

E

F

G

L

L

N

N

P

P

G

G

Q

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

W

Y

I

M

I

I

R

K

Q

D

A

A

E

E

K

E

A

R

G

G

Q

I

L

L

R

R

P

P

G

G

G

S

-

G

-

Q

V

V

I

I

V

V

E

E

G

P

T

T

A
x
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

V

M

V

I

A

A

S

A

A

S

L

R

G

G

Y

Y

R

R

T

L

V

I

I

L

V

T

I

M

P

P

E

A

T

Q

Q

M

S

S

Q

E

E

E

K

R

K

K

D

R

A

V

L

L

R

K

L

A

L

F

G

G

A

A

E

E

L

L

V

V

E

-

V

-

P

-

A

-

V

-

P

-

Y

L

R

T

N

D

P

P

N

E

N

R

Y

R

V

M

K

L

I

A

S

A

G

R

R

E

L

E

A

A

A

L

R

R

L

L

A

K

E

E

T

E

E

-

P

-

N

L

G

G

A

A

I

F

W

M

A

P

N

D

Q
|
Q

F

F

D

K

N

N

V

P

A

A

N

N

R

V

Q

R

A

A

H

H

I

Y

D

E

T

T

A

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

D

E

G

G

K

R

V

I

D

D

G

A

F

F

I

V

C
x
Y

A
x
G

V

S

G
|
G

S
x
T

G

G

T
|
T

L

I

A

T

G

G

V

V

A

G

E

R

G

Y

L

L

R

K

A

E

R

R

K

I

A

P

G

H

I

V

K

K

I

V

G

I

I

A

A

V

D
x
E

P

P

E

-

G

-

A

-

A

A

L

R

Y
x
S

N

N

F

V

Y

L

A

S

H

G

G

G

E

K

L

M

K

G

S

Q

S

H

G

G

S

-

I

-

T

F

E

Q

G
|
G

I

M

G

G

Q

P

G

G

R

F

I

I

T

P

A

E

N

N

L

L

E

D

G

L

F

S

T

L

P

L

D

D

F

G

A

V

Y

I

Q

Q

I

V

P

W

D

E

A

E

E

D

A

A

V

F

P

P

Y

L

V

A

F

R

E

R

L

L

I

A

E

R

K

E

E

E

G

G

I

L

C

F

I

L

G

G

G

M

S
|
S

T

S

G

G

I

G

N

I

V

V

A

W

G

A

A

A

V

L

R

Q

L

V

A

A

R

R

E

E

L

L

G

G

P

P

G

G

H

K

T

R

I

V

V

A

T

C

I

I

L

S

C
x
P

D

D

Y

G

G

G

N

W

R

K

Y

Y

Q

L

S

S

active site: K40 (= K44), S70 (≠ A72), E200 (≠ D210), S204 (≠ Y217), S263 (= S279)
binding (3s)-3-hydroxybutanoic acid: K40 (= K44), G71 (= G73), T73 (= T75), Q141 (= Q151), G219 (= G235)
binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ C182), G173 (≠ A183), G175 (= G185), T176 (≠ S186), T179 (= T189), G219 (= G235), S263 (= S279), P289 (≠ C305)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
39% identity, 88% coverage: 10:313/346 of query aligns to 6:297/302 of 2ecoA

query
sites
2ecoA

A

A

I

I

G

G

N

K

T

T

P

P

L

V

I

V

R

R

L

L

K

A

A

K

V

V

S

V

E

E

A

P

T

D

G

M

C

A

N

E

I

V

L

W

G

V

K

K

A

L

E

E

F

G

L

L

N

N

P

P

G

G

Q

G

S

S

V

I

K
|
K

D

D

R

R

A

P

A

A

L

W

W

Y

I

M

I

I

R

K

Q

D

A

A

E

E

K

E

A

R

G

G

Q

I

L

L

R

R

P

P

G

G

G

S

-

G

-

Q

V

V

I

I

V

V

E

E

G

P

T
|
T

A
x
S

G
|
G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

V

M

V

I

A

A

S

A

A

S

L

R

G

G

Y

Y

R

R

T

L

V

I

I

L

V

T

I

M

P

P

E

A

T

Q

Q

M

S

S

Q

E

E

E

K

R

K

K

D

R

A

V

L

L

R

K

L

A

L

F

G

G

A

A

E

E

L

L

V

V

E

-

V

-

P

-

A

-

V

-

P

-

Y

L

R

T

N

D

P

P

N

E

N

R

Y

R

V

M

K

L

I

A

S

A

G

R

R

E

L

E

A

A

A

L

R

R

L

L

A

K

E

E

T

E

E

-

P

-

N

L

G

G

A

A

I

F

W

M

A

P

N

D

Q
|
Q

F

F

D

K

N

N

V

P

A

A

N

N

R

V

Q

R

A

A

H

H

I

Y

D

E

T

T

A

G

P

P

E

E

I

L

W

Y

R

E

D

A

T

L

D

E

G

G

K

R

V

I

D

D

G

A

F

F

I

V

C
x
Y

A

G

V

S

G
|
G

S
x
T

G

G

T
|
T

L

I

A

T

G

G

V

V

A

G

E

R

G

Y

L

L

R

K

A

E

R

R

K

I

A

P

G

H

I

V

K

K

I

V

G

I

I

A

A

V

D
x
E

P

P

E

-

G

-

A

-

A

A

L

R

Y
x
S

N

N

F

V

Y

L

A

S

H

G

G

G

E

K

L

M

K

G

S

Q

S

H

G

G

S

-

I

-

T

F

E

Q

G
|
G

I

M

G

G

Q

P

G

G

R

F

I

I

T

P

A

E

N

N

L

L

E

D

G

L

F

S

T

L

P

L

D

D

F

G

A

V

Y

I

Q

Q

I

V

P

W

D

E

A

E

E

D

A

A

V

F

P

P

Y

L

V

A

F

R

E

R

L

L

I

A

E

R

K

E

E

E

G

G

I

L

C

F

I

L

G

G

G

M

S
|
S

T

S

G

G

I

G

N

I

V

V

A

W

G

A

A

A

V

L

R

Q

L

V

A

A

R

R

E

E

L

L

G

G

P

P

G

G

H

K

T

R

I

V

V

A

T

C

I

I

L

S

C
x
P

D
|
D

Y

G

G

G

N

W

R

K

Y

Y

Q

L

S

S

active site: K40 (= K44), S70 (≠ A72), E200 (≠ D210), S204 (≠ Y217), S263 (= S279)
binding 4-methyl valeric acid: K40 (= K44), T69 (= T71), G71 (= G73), T73 (= T75), Q141 (= Q151), G175 (= G185), T176 (≠ S186), G219 (= G235)
binding pyridoxal-5'-phosphate: K40 (= K44), N72 (= N74), Y172 (≠ C182), G175 (= G185), T176 (≠ S186), T179 (= T189), G219 (= G235), S263 (= S279), P289 (≠ C305), D290 (= D306)

3pc2A Full length structure of cystathionine beta-synthase from drosophila (see paper)
35% identity, 95% coverage: 3:329/346 of query aligns to 37:360/500 of 3pc2A

query
sites
3pc2A

V

I

L

T

P

P

S

N

V

I

L

L

E

E

A

V

I

I

G

G

N

C

T

T

P

P

L

L

I

V

R

K

L

L

K

N

A

N

V

I

S

P

E

A

A

S

T

D

G

G

-

I

-

E

C

C

N

E

I

M

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

Y

W

R

I

M

I

V

R

Q

Q

D

A

A

E

E

K

E

A

Q

G

G

Q

L

L

L

R

K

P

P

G

G

G

Y

V

T

I

I

V

I

E

E

G

P

T

T

A

S

G

G

N
|
N

T

T

G

G

I

I

G

G

L

L

A

A

V

M

V

A

A

C

S

A

A

V

L

K

G

G

Y

Y

R

K

T

C

V

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

K

V

D

S

A

A

L

L

R

R

L

T

L

L

G

G

A

A

E

K

L

I

V

I

E

R

V

T

P

P

A

T

-

E

V

A

P

A

Y

Y

R

D

N

S

P

P

N

E

N

G

Y

L

V

I

K

Y

I

V

S

-

G

-

R

-

L

-

A

A

A

Q

R

Q

L

L

A

Q

E

R

T

E

E

T

P

P

N

N

G

-

A

S

I

I

W

V

A

L

N

D

Q

Q

F

Y

D

R

N

N

V
x
A

A

G

N

N

R
x
P

Q
x
L

A

A

H

H

I
x
Y

D

D

T

G

T

T

A

A

P

A

E

E

I

I

W

L

R

W

D

Q

T

L

D

D

G

N

K

K

V

V

D

D

G

M

F

I

I

V

C

V

A

S

V
x
A

G
|
G

S
x
T

G
x
A

G

G

T
|
T

L

I

A

S

G

G

V

I

A

G

E
x
R

G

K

L

I

R

K

A

E

R

Q

K

V

A

P

G

S

I

C

K

Q

I

I

G

V

I

G

A

V

D

D

P

P

E

Y

G

G

A

S

A

I

L

L

Y

A

N

R

F

-

Y

-

A

-

H

P

G

A

E

E

L

L

K

N

S

K

S

T

G

D

S

V

S

Q

I

F

-

Y

-

E

T

V

E

E

G
|
G

I

I

G

G

Q

Y

G

D

R

F

I

P

T

P

A

T

N

V

L

F

E

D

G

D

F

T

T

V

P

V

D

D

F

V

A

W

Y

T

Q

K

I

I

P

G

D

D

A

S

E

D

A

C

V

F

P

P

Y

M

V

S

F

R

E

R

L

L

I

N

E

A

K

E

E

E

G

G

I

L

C

L

I

C

G

G

S
|
S

T

S

G

G

I

G

N

A

V

M

A

H

G

A

A

A

V

L

R

E

L

H

A

A

R

R

E

K

L

L

G

K

P

K

G

G

H

Q

T

R

I

C

V

V

T

V

I

I

L

L

C
x
P

D
|
D

Y

G

G

I

N

R

R

N

Y

Y

Q

M

S

T

K

K

L

F

F

V

N

S

P

D

D

N

F

W

L

M

A

E

S

A

K

R

G

N

L

F

P

K

V

E

P

P

active site: K80 (= K44), S310 (= S279)
binding protoporphyrin ix containing fe: A187 (≠ V155), P190 (≠ R158), L191 (≠ Q159), Y194 (≠ I162), R227 (≠ E195)
binding pyridoxal-5'-phosphate: K80 (= K44), N110 (= N74), A216 (≠ V184), G217 (= G185), T218 (≠ S186), A219 (≠ G187), T221 (= T189), G266 (= G235), S310 (= S279), P336 (≠ C305), D337 (= D306)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 12, 13, 14, 15, 16, 24, 25, 26

3pc4A Full length structure of cystathionine beta-synthase from drosophila in complex with serine (see paper)
35% identity, 95% coverage: 3:329/346 of query aligns to 39:362/504 of 3pc4A

query
sites
3pc4A

V

I

L

T

P

P

S

N

V

I

L

L

E

E

A

V

I

I

G

G

N

C

T

T

P

P

L

L

I

V

R

K

L

L

K

N

A

N

V

I

S

P

E

A

A

S

T

D

G

G

-

I

-

E

C

C

N

E

I

M

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

Y

W

R

I

M

I

V

R

Q

Q

D

A

A

E

E

K

E

A

Q

G

G

Q

L

L

L

R

K

P

P

G

G

G

Y

V

T

I

I

V

I

E

E

G

P

T
|
T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

V

M

V

A

A

C

S

A

A

V

L

K

G

G

Y

Y

R

K

T

C

V

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

K

V

D

S

A

A

L

L

R

R

L

T

L

L

G

G

A

A

E

K

L

I

V

I

E

R

V

T

P

P

A

T

-

E

V

A

P

A

Y

Y

R

D

N

S

P

P

N

E

N

G

Y

L

V

I

K

Y

I

V

S

-

G

-

R

-

L

-

A

A

A

Q

R

Q

L

L

A

Q

E

R

T

E

E

T

P

P

N

N

G

-

A

S

I

I

W

V

A

L

N

D

Q
|
Q

F

Y

D

R

N

N

V
x
A

A

G

N

N

R
x
P

Q
x
L

A

A

H

H

I
x
Y

D

D

T

G

T

T

A

A

P

A

E

E

I

I

W

L

R

W

D

Q

T

L

D

D

G

N

K

K

V

V

D

D

G

M

F

I

I

V

C

V

A

S

V
x
A

G
|
G

S
x
T

G
x
A

G

G

T
|
T

L

I

A

S

G

G

V

I

A

G

E
x
R

G

K

L

I

R

K

A

E

R

Q

K

V

A

P

G

S

I

C

K

Q

I

I

G

V

I

G

A

V

D

D

P

P

E

Y

G

G

A

S

A

I

L

L

Y

A

N

R

F

-

Y

-

A

-

H

P

G

A

E

E

L

L

K

N

S

K

S

T

G

D

S

V

S

Q

I

F

-

Y

-

E

T

V

E

E

G
|
G

I
|
I

G

G

Q
x
Y

G

D

R

F

I

P

T

P

A
x
T

N

V

L

F

E

D

G

D

F

T

T

V

P

V

D

D

F

V

A

W

Y

T

Q

K

I

I

P

G

D

D

A

S

E

D

A

C

V

F

P

P

Y

M

V

S

F

R

E

R

L

L

I

N

E

A

K

E

E

E

G

G

I

L

C

L

I

C

G

G

S
|
S

T

S

G

G

I

G

N

A

V

M

A

H

G

A

A

A

V

L

R

E

L

H

A

A

R

R

E

K

L

L

G

K

P

K

G

G

H

Q

T

R

I

C

V

V

T

V

I

I

L

L

C
x
P

D
|
D

Y

G

G

I

N

R

R

N

Y

Y

Q

M

S

T

K

K

L

F

F

V

N

S

P

D

D

N

F

W

L

M

A

E

S

A

K

R

G

N

L

F

P

K

V

E

P

P

active site: K82 (= K44), S312 (= S279)
binding protoporphyrin ix containing fe: A189 (≠ V155), P192 (≠ R158), L193 (≠ Q159), Y196 (≠ I162), R229 (≠ E195), T276 (≠ A243)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: K82 (= K44), T109 (= T71), S110 (≠ A72), N112 (= N74), T113 (= T75), Q185 (= Q151), A218 (≠ V184), G219 (= G185), T220 (≠ S186), A221 (≠ G187), T223 (= T189), G268 (= G235), I269 (= I236), Y271 (≠ Q238), S312 (= S279), P338 (≠ C305), D339 (= D306)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

3pc3A Full length structure of cystathionine beta-synthase from drosophila in complex with aminoacrylate (see paper)
35% identity, 95% coverage: 3:329/346 of query aligns to 39:362/504 of 3pc3A

query
sites
3pc3A

V

I

L

T

P

P

S

N

V

I

L

L

E

E

A

V

I

I

G

G

N

C

T

T

P

P

L

L

I

V

R

K

L

L

K

N

A

N

V

I

S

P

E

A

A

S

T

D

G

G

-

I

-

E

C

C

N

E

I

M

L

Y

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

G

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

I

A

G

L

Y

W

R

I

M

I

V

R

Q

Q

D

A

A

E

E

K

E

A

Q

G

G

Q

L

L

L

R

K

P

P

G

G

G

Y

V

T

I

I

V

I

E

E

G

P

T
|
T

A
x
S

G

G

N
|
N

T
|
T

G

G

I

I

G

G

L

L

A

A

V

M

V

A

A

C

S

A

A

V

L

K

G

G

Y

Y

R

K

T

C

V

I

I

I

V

V

I

M

P

P

E

E

T

K

Q

M

S

S

Q

N

E

E

K

K

K

V

D

S

A

A

L

L

R

R

L

T

L

L

G

G

A

A

E

K

L

I

V

I

E

R

V

T

P

P

A

T

-

E

V

A

P

A

Y

Y

R

D

N

S

P

P

N

E

N

G

Y

L

V

I

K

Y

I

V

S

-

G

-

R

-

L

-

A

A

A

Q

R

Q

L

L

A

Q

E

R

T

E

E

T

P

P

N

N

G

-

A

S

I

I

W

V

A

L

N

D

Q
|
Q

F

Y

D

R

N

N

V
x
A

A

G

N

N

R
x
P

Q
x
L

A

A

H

H

I
x
Y

D

D

T

G

T

T

A

A

P

A

E

E

I

I

W

L

R

W

D

Q

T

L

D

D

G

N

K

K

V

V

D

D

G

M

F

I

I

V

C

V

A

S

V
x
A

G
|
G

S
x
T

G
x
A

G

G

T
|
T

L

I

A

S

G

G

V

I

A

G

E
x
R

G

K

L

I

R

K

A

E

R

Q

K

V

A

P

G

S

I

C

K

Q

I

I

G

V

I

G

A

V

D

D

P

P

E

Y

G

G

A

S

A

I

L

L

Y

A

N

R

F

-

Y

-

A

-

H

P

G

A

E

E

L

L

K

N

S

K

S

T

G

D

S

V

S

Q

I

F

-

Y

-

E

T

V

E

E

G
|
G

I
|
I

G

G

Q

Y

G

D

R

F

I

P

T

P

A

T

N

V

L

F

E

D

G

D

F

T

T

V

P

V

D

D

F

V

A

W

Y

T

Q

K

I

I

P

G

D

D

A

S

E

D

A

C

V

F

P

P

Y

M

V

S

F

R

E

R

L

L

I

N

E

A

K

E

E

E

G

G

I

L

C

L

I

C

G

G

S
|
S

T

S

G

G

I

G

N

A

V

M

A

H

G

A

A

A

V

L

R

E

L

H

A

A

R

R

E

K

L

L

G

K

P

K

G

G

H

Q

T

R

I

C

V

V

T

V

I

I

L

L

C
x
P

D
|
D

Y

G

G

I

N

R

R

N

Y

Y

Q

M

S

T

K

K

L

F

F

V

N

S

P

D

D

N

F

W

L

M

A

E

S

A

K

R

G

N

L

F

P

K

V

E

P

P

active site: K82 (= K44), S312 (= S279)
binding protoporphyrin ix containing fe: A189 (≠ V155), P192 (≠ R158), L193 (≠ Q159), Y196 (≠ I162), R229 (≠ E195)
binding 2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]acrylic acid: K82 (= K44), T109 (= T71), S110 (≠ A72), N112 (= N74), T113 (= T75), Q185 (= Q151), A218 (≠ V184), G219 (= G185), T220 (≠ S186), A221 (≠ G187), T223 (= T189), G268 (= G235), I269 (= I236), S312 (= S279), P338 (≠ C305), D339 (= D306)

Sites not aligning to the query:

binding protoporphyrin ix containing fe: 14, 15, 16, 17, 18, 26, 27, 28

2bhtA Crystal structure of o-acetylserine sulfhydrylase b (see paper)
35% identity, 90% coverage: 4:313/346 of query aligns to 1:288/294 of 2bhtA

query
sites
2bhtA

L

M

P

S

S

T

V

L

L

E

E

Q

A

T

I

I

G

G

N

N

T

T

P

P

L

L

I

V

R

K

L

L

K

Q

A

R

V

M

S

G

E

P

A

D

T

N

G

G

C

S

N

E

I

V

L

W

G

L

K

K

A

L

E

E

F

G

L

N

N

N

P

P

G

A

Q

G

S

S

V

V

K
|
K

D

D

R

R

A

A

L

L

W

S

I

M

I

I

R

V

Q

E

A

A

E

E

K

K

A

R

G

G

Q

R

L

I

R

K

P

P

G

G

G

D

V

V

I

L

V

I

E

E

G

A

T

T

A
x
S

G

G

N
|
N

T

T

G

G

I

I

G

A

L

L

A

A

V

M

V

I

A

A

S

A

A

L

L

K

G

G

Y

Y

R

R

T

M

V

K

I

L

V

L

I

M

P

P

E

D

T

N

Q

M

S

S

Q

Q

E

E

K

R

D

A

A

A

L

M

R

R

L

A

L

Y

G

G

A

A

E

E

L

L

V

I

E

L

V

V

P

-

A

-

V

-

P

-

Y

-

R

-

N

-

P

-

N

-

Y

-

V

T

K

K

I

E

S

Q

G

G

R

M

L

E

A

G

A

A

R

R

-

D

-

L

-

A

L

L

A

E

E

M

T

A

E

N

P

R

N

G

G

E

A

G

I

K

W

L

A

L

N

D

Q

Q

F

F

D

N

N

N

V

P

A

D

N

N

R

P

Q

K

A

A

H

H

I

Y

D

T

T

T

A

G

P

P

E

E

I

I

W

W

R

Q

D

Q

T

T

D

G

G

G

K

R

V

I

D

T

G

H

F

F

I

V

C

S

A

S

V
x
M

G
|
G

S
x
T

G
x
T

G

G

T
|
T

L

I

A

T

G

G

V

V

A

S

E

E

G

F

L

M

R

R

A

E

R

Q

K

S

A

K

G

P

I

V

K

T

I

I

-

V

G

G

I

L

A
x
Q

D

P

P

E

E

E

G
|
G

A

S

L

I

Y

P

N
x
G

F

-

Y

-

A

-

H

-

G

-

E

-

L

I

K

R

S

R

S

W

G

P

S

T

S

E

I

Y

T

L

E

P

G

G

I

I

G

F

Q

N

G

A

R

S

I

L

T

V

A

-

N

-

L

-

E

-

G

-

F

-

T

-

P

-

D

D

F

E

A

V

Y

L

Q

D

I

I

P

H

D

Q

A

R

E

D

A

A

V

E

P

N

Y

T

V

M

F

R

E

E

L

L

I

A

E

V

K

R

E

E

G

G

I

I

C

F

I

C

G

G

G

V

S
|
S

T

S

G

G

I

G

N

A

V

V

A

A

G

G

A

A

V

L

R

R

L

V

A

A

R

K

E

A

L

-

G

N

P

P

G

D

H

A

T

V

I

V

V

V

T

A

I

I

L

I

C
|
C

D

D

Y

R

G

G

N

D

R

R

Y

Y

Q

L

S

S

active site: K41 (= K44), S69 (≠ A72), Q199 (≠ A209), G203 (= G213), S255 (= S279), C280 (= C305)
binding pyridoxal-5'-phosphate: K41 (= K44), N71 (= N74), M173 (≠ V184), G174 (= G185), T175 (≠ S186), T176 (≠ G187), T178 (= T189), G208 (≠ N218), S255 (= S279), C280 (= C305)

7mfjBBB Beta-cyanoalanine synthase (see paper)
32% identity, 89% coverage: 3:311/346 of query aligns to 13:319/319 of 7mfjBBB

query
sites
7mfjBBB

V

I

L

T

P

P

S

S

V

A

L

L

E

D

A

L

I

I

G

G

N

N

T

T

P

P

L

L

I

I

R

A

L

L

K

D

A

R

V

L

S

W

E

P

A

G

T

P

G

G

C

-

N

R

I

L

L

L

G

A

K

K

A

C

E

E

F

F

L

L

N

N

P

P

G

T

Q

A

S

S

V

L

K
|
K

D

D

R

R

A

S

L

Y

W

Y

I

M

I

I

R

A

Q

K

A

A

E

K

K

E

A

S

G

G

Q

Q

L

L

R

K

P

D

G

G

G

E

V

S

I

V

V

I

E

E

G

V

T

T

A

S

G

G

N
|
N

T

Q

G

G

I

G

G

G

L

I

A

A

V

C

V

V

A

T

S

A

A

V

L

M

G

G

Y

H

R

P

T

F

V

T

I

V

V

T

I

M

P

S

E

K

T

G

Q

N

S

S

Q

P

E

Q

K

R

K

A

D

I

A

M

L

M

R

N

L

A

L

L

G

G

A

A

E

N

L

V

V

I

E

L

V

V

P

D

A

Q

V

V

P

T

Y

G

R

K

N

-

P

P

N

G

N

N

Y

V

V

T

-

A

-

D

K

D

I

V

S

A

G

A

R

A

L

E

A

E

A

T

R

A

L

M

A

K

E

I

T

R

E

E

P

E

N

T

G

N

A

A

I

Y

W

Y

A

V

N

D

Q

Q

F

F

D

N

N

N

V

P

A

T

N

N

R

C

Q

L

A

A

H

H

I

Y

D

E

T

T

A

G

P

P

E

E

I

I

W

W

R

R

D

Q

T

T

D

N

G

G

K

R

V

I

D

D

G

A

F

F

I

L

C

V

A

G

V

C

G
|
G

S
x
T

G
|
G

G

G

T
x
C

L

F

A

V

G

G

V

T

A

S

E

K

G

F

L

L

R

K

A

E

R

K

K

N

A

P

G

N

I

V

K

R

I

C

G

F

I

V

A

V

D

E

P

P

E

E

G

G

A

C

A

Q

L

-

Y

-

N

-

F

-

Y

-

A

-

H

-

G

-

E

-

L

-

K

P

S

I

S

A

G

G

S

C

S

T

I

I

T

T

-

K

-

P

-

L

-

H

-

L

E

Q

G
|
G

I

S

G

G

Q

Y

G

G

R

C

I

V

T

P

A

T

N

L

L

F

E

D

G

K

F

K

T

V

P

Y

D

N

F

D

A

S

Y

I

Q

S

I

V

P

S

D

D

A

E

E

E

A

A

V

I

P

E

Y

Y

V

R

F

K

E

L

L

L

I

G

E

Q

K

K

E

E

G

G

I

L

C

F

I

C

G

G

G

F

S
x
T

T

T

G

G

I

G

N

N

V

I

A

A

G

A

A

A

V

I

R

K

L

L

A

L

R

K

-

S

-

G

E

Q

L

L

G

P

P

K

G

D

H

A

T

W

I

V

V

V

T

T

I

I

L

L

C
|
C

D

D

Y

S

G

G

N

L

R

K

Y

Y

binding pyridoxal-5'-phosphate: K53 (= K44), N83 (= N74), G195 (= G185), T196 (≠ S186), G197 (= G187), C199 (≠ T189), G241 (= G235), T285 (≠ S279), C313 (= C305)

Query Sequence

>WP_011975521.1 NCBI__GCF_000017145.1:WP_011975521.1
MPVLPSVLEAIGNTPLIRLKAVSEATGCNILGKAEFLNPGQSVKDRAALWIIRQAEKAGQ
LRPGGVIVEGTAGNTGIGLAVVASALGYRTVIVIPETQSQEKKDALRLLGAELVEVPAVP
YRNPNNYVKISGRLAARLAETEPNGAIWANQFDNVANRQAHIDTTAPEIWRDTDGKVDGF
ICAVGSGGTLAGVAEGLRARKAGIKIGIADPEGAALYNFYAHGELKSSGSSITEGIGQGR
ITANLEGFTPDFAYQIPDAEAVPYVFELIEKEGICIGGSTGINVAGAVRLARELGPGHTI
VTILCDYGNRYQSKLFNPDFLASKGLPVPAWLKTASNIAVPYEPAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory