SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011975608.1 NCBI__GCF_000017145.1:WP_011975608.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
43% identity, 94% coverage: 23:435/440 of query aligns to 22:431/435 of Q5F8J4

query
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L45 (≠ R46) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ RD 46:47) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
39% identity, 98% coverage: 3:435/440 of query aligns to 1:429/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G11), L12 (= L12), G13 (= G13), N14 (≠ T14), V15 (= V15), V45 (≠ A45), R46 (= R46), R47 (≠ D47), R52 (= R52), I63 (≠ A65), L78 (= L80), M79 (= M81), P84 (= P87), A102 (= A105), K104 (= K107), G306 (= G304), T310 (= T308)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
38% identity, 72% coverage: 5:320/440 of query aligns to 5:307/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D203), K194 (= K207)
binding lysine: R215 (≠ E228), G219 (≠ N232)

Sites not aligning to the query:

binding lysine: 394, 395

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
38% identity, 75% coverage: 3:331/440 of query aligns to 2:315/331 of 6a0sA

query
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L

A

L

V

E

S

A

L

-

A

-

S

-

Q

-

G

-

I

-

D

D

V

L

F

V

V

V

E

E

L
x
A

M
|
M

G

G

T

V

G

E

D

A

P

P

A

-

Y

L

A

R

S

L

V

V

K

L

A

P

A

A

L

L

T

E

H

A

G

G

V

I

H

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

A

E

H

A

G

W

I

E

E

S

L

L

A

R

E

P

I

F

A

A

E

E

K

E

H

-

G

-

A

G

L

L

L

I

N

Y

Y

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

A

F

L

L

R

-

E

E

S

T

M

L

T

R

G

G

N

S

T

E

I

L

S

L

R

E

V

L

Y

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

E

E

K

K

E

-

G

G

L

R

S

T

F

Y

E

A

A

E

C

A

L

L

K

L

E

E

A

A

Q

Q

R

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

A

T

F

L

D
|
D

I

V

E

E

G

G

N

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

S

T

I

L

L

L

T

A

S

R

L

L

A

L

F

V

G

D

T

P

A

G

I

F

A

P

A

F

D

A

D

E

I

V

Y

E

L

A

E

Q

G

G

I

I

T

A

N

R

I

L

S

T

I

P

E

E

D

V

I

L

Q

Q

A

K

A

A

A

E

D

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

R

L

T

F

E

G

S

E

G

G

I

G

E

R

Q

W

R

R

-

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

H

Q

D

D

T

H

V

P

I

L

A

A

Q

R

V

A

D

R

G

G

V

-

T

-

N

N

A

A

V

L

A

W

I

V

E

R

S

A

D

R

I

P

L

L

G

G

E

E

L

A

L

F

M

V

V

T

G

G

P

P

G

G

A
|
A

G
|
G

G

G

D

G

A

A

T
|
T

A

A

S

S

A

G

V

L

L

F

G

A

D

D

I

L

A

L

D

R

I

F

A

L

K

S

S

G

R

A

P

P

G

G

A

-

Q

-

H

H

V

L

P

P

A

A

active site: D191 (= D203), K195 (= K207)
binding l-homoserine: K99 (= K107), N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ T44), V43 (≠ A45), R44 (= R46), D45 (= D47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (= M81), A97 (= A105), K99 (= K107), G177 (= G189), E180 (= E192), A289 (= A303), G290 (= G304), T294 (= T308)

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
38% identity, 75% coverage: 3:331/440 of query aligns to 2:315/331 of 2ejwA

query
sites
2ejwA

D

E

A

A

L

L

K

K

I

I

G

A

I

L

A

L

G

G

L

G

G

G

T

T

V

V

G

G

A

S

S

A

L

F

V

Y

R

N

I

L

L

V

Q

L

D

E

R

R

H

A

E

E

T

E

L

L

A

-

T

S

T

A

C

F

G

G

R

V

A

V

I

P

E

R

I

F

T

L

A

G

V

V

T

L

A

V

R

R

D

D

R

P

S

R

K

K

D

P

R

R

G

A

V

I

-

P

-

Q

D

E

L

L

A

L

G

R

I

A

T

E

W

P

F

F

D

D

D

L

A

L

V

E

S

A

L

-

A

-

S

-

Q

-

G

-

I

-

D

D

V

L

F

V

V

V

E

E

L

A

M

M

G

G

T

V

G

E

D

A

P

P

A

-

Y

L

A

R

S

L

V

V

K

L

A

P

A

A

L

L

T

E

H

A

G

G

V

I

H

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

A

E

H

A

G

W

I

E

E

S

L

L

A

R

E

P

I

F

A

A

E

E

K

E

H

-

G

-

A

G

L

L

L

I

N

Y

Y

H

E
|
E

A

A

A

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

V

A

I

L

K

S

A

F

L

L

R

-

E

E

S

T

M

L

T

R

G

G

N

S

T

E

I

L

S

L

R

E

V

L

Y

H

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

E

E

K

K

E

-

G

G

L

R

S

T

F

Y

E

A

A

E

C

A

L

L

K

L

E

E

A

A

Q

Q

R

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

A

T

F

L

D

D

I

V

E

E

G

G

N

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

S

T

I

L

L

L

T

A

S

R

L

L

A

L

F

V

G

D

T

P

A

G

I

F

A

P

A

F

D

A

D

E

I

V

Y

E

L

A

E

Q

G

G

I

I

T

A

N

R

I

L

S

T

I

P

E

E

D

V

I

L

Q

Q

A

K

A

A

A

E

D

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

R

L

T

F

E

G

S

E

G

G

I

G

E

R

Q

W

R

R

-

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

H

Q

D

D

T

H

V

P

I

L

A

A

Q

R

V

A

D

R

G

G

V

-

T

-

N

N

A

A

V

L

A

W

I

V

E

R

S

A

D

R

I

P

L

L

G

G

E

E

L

A

L

F

M

V

V

T

G

G

P

P

G

G

A

A

G

G

D

G

A

A

T

T

A

A

S

S

A

G

V

L

L

F

G

A

D

D

I

L

A

L

D

R

I

F

A

L

K

S

S

G

R

A

P

P

G

G

A

-

Q

-

H

H

V

L

P

P

A

A

active site: D191 (= D203), K195 (= K207)
binding magnesium ion: E121 (= E131), V124 (= V134), M125 (≠ A135), A126 (≠ G136), T128 (≠ I138)

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
38% identity, 75% coverage: 3:331/440 of query aligns to 2:315/332 of 6a0tB

query
sites
6a0tB

D

E

A

A

L

L

K

K

I

I

G

A

I

L

A

L

G
|
G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

A

S

S

A

L

F

V

Y

R

N

I

L

L

V

Q

L

D

E

R

R

H

A

E

E

T

E

L

L

A

-

T

S

T

A

C

F

G

G

R

V

A

V

I

P

E

R

I

F

T

L

A

G

V

V

T
x
L

A
x
V

R
|
R

D
|
D

R

P

S

R

K
|
K

D

P

R
|
R

G

A

V

I

-

P

-

Q

D

E

L

L

A

L

G

R

I

A

T

E

W

P

F

F

D

D

D

L

A

L

V

E

S

A

L

-

A

-

S

-

Q

-

G

-

I

-

D

D

V

L

F

V

V

V

E

E

L
x
A

M
|
M

G
|
G

G

G

T

V

G

E

D

A

P

P

A

-

Y

L

A

R

S

L

V

V

K

L

A

P

A

A

L

L

T

E

H

A

G

G

V

I

H

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

A

A

A

E

H

A

G

W

I

E

E

S

L

L

A

R

E

P

I

F

A

A

E

E

K

E

H

-

G

-

A

G

L

L

L

I

N

Y

Y

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

V

A

I

L

K

S

A

F

L

L

R

-

E

E

S

T

M

L

T

R

G

G

N

S

T

E

I

L

S

L

R

E

V

L

Y

H

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

K

E

M

M

E

E

K

K

E

-

G

G

L

R

S

T

F

Y

E

A

A

E

C

A

L

L

K

L

E

E

A

A

Q

Q

R

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

A

T

F

L

D
|
D

I

V

E

E

G

G

N

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

S

T

I

L

L

L

T

A

S

R

L

L

A

L

F

V

G

D

T

P

A

G

I

F

A

P

A

F

D

A

D

E

I

V

Y

E

L

A

E

Q

G

G

I

I

T

A

N

R

I

L

S

T

I

P

E

E

D

V

I

L

Q

Q

A

K

A

A

A

E

D

A

L

R

G

G

Y

E

R

R

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

S

R

L

T

F

E

G

S

E

G

G

I

G

E

R

Q

W

R

R

-

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

H

Q

D

D

T

H

V

P

I

L

A

A

Q

R

V

A

D

R

G

G

V

-

T

-

N

N

A

A

V

L

A

W

I

V

E

R

S

A

D

R

I

P

L

L

G

G

E

E

L

A

L

F

M

V

V

T

G

G

P

P

G

G

A
|
A

G
|
G

G

G

D

G

A

A

T
|
T

A

A

S

S

A

G

V

L

L

F

G

A

D

D

I

L

A

L

D

R

I

F

A

L

K

S

S

G

R

A

P

P

G

G

A

-

Q

-

H

H

V

L

P

P

A

A

active site: D191 (= D203), K195 (= K207)
binding l-homoserine: N150 (= N161), G151 (= G162), T152 (= T163), Y178 (= Y190), E180 (= E192), D186 (= D198), K195 (= K207)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G11), G11 (≠ L12), G12 (= G13), T13 (= T14), V14 (= V15), L42 (≠ T44), V43 (≠ A45), R44 (= R46), D45 (= D47), K48 (= K50), R50 (= R52), A73 (≠ L80), M74 (= M81), G75 (= G82), A97 (= A105), N98 (= N106), G177 (= G189), E180 (= E192), A289 (= A303), G290 (= G304), T294 (= T308)

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
33% identity, 71% coverage: 5:316/440 of query aligns to 1:292/300 of 7f4cA

query
sites
7f4cA

L

M

K

K

I

L

G

L

I

L

A
x
F

G
|
G

L
x
Y

G
|
G

T
x
N

V
|
V

G

G

A

K

S

A

L

F

V

R

R

K

I

L

L

L

Q

H

D

E

R

K

H

R

-

S

-

P

E

E

T

L

L

N

A

D

T

V

T

I

C

I

G

G

R

G

A

I

I

V

E
x
T

I
x
R

T
x
R

A

G

V

I

T

M

A

L

R

Q

D

D

R

K

S

-

K

E

D

D

R

F

G

T

V

P

D

D

L

L

A

E

G

G

I

-

T

-

W

-

F

-

D

-

D

D

A

V

V

F

S

K

L

A

A

F

S

E

Q

K

G

I

G

K

I

P

D

D

V

I

F

I

V

V

E

D

L
x
V

M
x
S

G
x
S

-

A

-

N

-

Y

G

N

T

N

G

G

D

E

P

P

A

S

Y

L

A

S

S

L

V

Y

K

K

A

E

A

A

L

I

T

K

H

D

G

G

V

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

A

L

H

A

G

F

I

N

E

E

L

I

A

F

E

S

I

L

A

A

E

R

K

S

H

K

G

G

A

V

L

K

L

I

N

G

Y

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

A

L

L

Y

R

R

E

-

S

V

M

L

T

P

G

G

N

S

T

R

I

V

S

I

R

K

V

I

Y

R

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

K

L

M

M

E

N

K

K

E

-

G

G

L

V

S

S

F

F

E

E

A

E

C

A

L

L

K

K

E

E

A

A

Q

Q

R

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

A

T

F

L

D

D

I

I

E

N

G

G

N

F

D

D

T

A

A

A

H

A

K

K

L

I

S

T

I

I

L

L

T

A

S

N

L

F

A

M

F

I

G

G

T

N

A

S

I

V

A

T

A

I

D

K

D

D

I

V

Y

K

L

F

E

E

G

G

I

I

T

N

N

R

I

-

S

-

I

-

E

-

D

-

I

-

Q

-

A

-

D

D

L

L

G

-

Y

-

R

K

I

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

R

E

T

K

E

E

S

V

G

W

I

-

E

-

Q

-

R

-

V

V

H

K

P

P

T

L

M

P

V

I

P

S

H

Q

D

D

T

D

V

P

I

L

A

Y

Q

N

V

V

D

D

G

G

V

V

T

E

N

N

A

A

V

L

A

E

I

I

E

T

S

T

D

D

I

I

L

-

G

Q

E

S

L

I

L

L

M

I

V

R

G

G

P

P

G

G

A

A

G
|
G

G

P

D

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

L

L

G

S

D

D

I

L

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ A10), G7 (= G11), Y8 (≠ L12), G9 (= G13), N10 (≠ T14), V11 (= V15), T37 (≠ E39), R38 (≠ I40), R39 (≠ T41), V72 (≠ L80), S73 (≠ M81), S74 (≠ G82), T100 (≠ A105), K102 (= K107), G127 (≠ A132), S131 (≠ G136), E185 (= E192), G280 (= G304), A284 (≠ T308)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
33% identity, 71% coverage: 5:316/440 of query aligns to 1:296/304 of F9VNG5

query
sites
F9VNG5

L

M

K

K

I

L

G

L

I

L

A

F

G

G

L
x
Y

G

G

T
x
N

V
|
V

G

G

A

K

S

A

L

F

V

R

R

K

I

L

L

L

Q

H

D

E

R

K

H

R

-

S

-

P

E

E

T

L

L

N

A

D

T

V

T

I

C

I

G

G

R

G

A

I

I

V

E

T

I
x
R

T
x
R

A

G

V

I

T

M

A

L

R

Q

D

D

R

K

S

-

K

E

D

D

R

F

G

T

V

P

D

D

L

L

A

E

G

G

I

-

T

-

W

-

F

-

D

-

D

D

A

V

V

F

S

K

L

A

A

F

S

E

Q

K

G

I

G

K

I

P

D

D

V

I

F

I

V

V

E

D

L

V

M
x
S

G

S

-

A

-

N

-

Y

G

N

T

N

G

G

D

E

P

P

A

S

Y

L

A

S

S

L

V

Y

K

K

A

E

A

A

L

I

T

K

H

D

G

G

V

V

H

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

A

L

H

A

G

F

I

N

E

E

L

I

A

F

E

S

I

L

A

A

E

R

K

S

H

K

G

G

A

V

L

K

L

I

N

G

Y

F

E

Q

A

G

A

T

V

V

A

M

G

S

G

G

I

T

P

P

V

S

I

I

K

N

A

L

L

Y

R

R

E

-

S

V

M

L

T

P

G

G

N

S

T

R

I

V

S

I

R

K

V

I

Y

R

G

G

I

I

M

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

K

L

M

M

E

N

K

K

E

-

G

G

L

V

S

S

F

F

E

E

A

E

C

A

L

L

K

K

E

E

A

A

Q

Q

R

R

L

R

G
|
G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

A

T

F

L

D

D

I

I

E

N

G

G

N

F

D

D

T

A

A

A

H

A

K

K

L

I

S

T

I

I

L

L

T

A

S

N

L

F

A

M

F

I

G

G

T

N

A

S

I

V

A

T

A

I

D

K

D

D

I

V

Y

K

L

F

E

E

G

G

I

I

T

N

N

-

I

-

S

-

I

-

E

R

D

D

I

L

Q

P

A

K

A

N

A

E

D

-

L

-

G

-

Y

-

R

K

I

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

R

E

T

K

E

E

S

V

G

W

I

-

E

-

Q

-

R

-

V

V

H

K

P

P

T

L

M

P

V

I

P

S

H

Q

D

D

T

D

V

P

I

L

A

Y

Q

N

V

V

D

D

G

G

V

V

T

E

N

N

A

A

V

L

A

E

I

I

E

T

S

T

D

D

I

I

L

-

G

Q

E

S

L

I

L

L

M

I

V

R

G

G

P

P

G

G

A

A

G
|
G

G

P

D

V

A

N

T

A

A

A

S

Y

A

G

V

A

L

L

G

S

D

D

I

L

Y8 (≠ L12) binding NADP(+)
N10 (≠ T14) binding NADP(+)
V11 (= V15) binding NADP(+)
R38 (≠ I40) binding NADP(+)
R39 (≠ T41) binding NADP(+)
S73 (≠ M81) binding NADP(+)
T100 (≠ A105) binding NADP(+)
K102 (= K107) binding NADP(+)
G182 (= G189) binding NADP(+)
E185 (= E192) binding NADP(+)
G284 (= G304) binding NADP(+)

Sites not aligning to the query:

303 modified: Interchain

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
32% identity, 71% coverage: 5:316/440 of query aligns to 1:294/302 of 5x9dA

query
sites
5x9dA

L

M

K

K

I

L

G

L

I

L

A
x
F

G
|
G

L
x
Y

G
|
G

T
x
N

V
|
V

G

G

A

K

S

A

L

F

V

R

R

K

I

L

L

L

Q

H

D

E

R

K

H

R

-

S

-

P

E

E

T

L

L

N

A

D

T

V

T

I

C

I

G

G

R

G

A

I

I

V

E
x
T

I
x
R

T

R

A

G

V

I

T

M

A

L

R

Q

D

D

R

K

S

-

K

E

D

D

R

F

G

T

V

P

D

D

L

L

A

E

G

G

I

-

T

-

W

-

F

-

D

-

D

D

A

V

V

F

S

K

L

A

A

F

S

E

Q

K

G

I

G

K

I

P

D

D

V

I

F

I

V

V

E

D

L
x
V

M
x
S

G
x
S

-

A

-

N

-

Y

G

N

T

N

G

G

D

E

P
|
P

A

S

Y

L

A

S

S

L

V

Y

K

K

A

E

A

A

L

I

T

K

H

D

G

G

V

V

H

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

A

L

H

A

G

F

I

N

E

E

L

I

A

F

E

S

I

L

A

A

E

R

K

S

H

K

G

G

A

V

L

K

L

I

N

G

Y

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

V

S

I

I

K

N

A

L

L

Y

R

R

E

-

S

V

M

L

T

P

G

G

N

S

T

R

I

V

S

I

R

K

V

I

Y

R

G

G

I

I

M

L

N
|
N

G
|
G

T
|
T

C

T

N

N

Y

F

I

I

L

L

T

T

K

L

M

M

E

N

K

K

E

-

G

G

L

V

S

S

F

F

E

E

A

E

C

A

L

L

K

K

E

E

A

A

Q

Q

R

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

A

T

F

L

D
|
D

I

I

E

N

G

G

N

F

D
|
D

T

A

A

A

H

A

K
|
K

L

I

S

T

I

I

L

L

T

A

S

N

L

F

A

M

F

I

G

G

T

N

A

S

I

V

A

T

A

I

D

K

D

D

I

V

Y

K

L

F

E

E

G

G

I

I

T

N

N

R

I

D

S

P

I

K

E

N

D

E

I

-

Q

-

A

-

D

-

L

-

G

-

Y

-

R

K

I

I

K

K

L

L

L

I

G

A

V

Y

A

A

Q

D

R

E

T

K

E

E

S

V

G

W

I

-

E

-

Q

-

R

-

V

V

H

K

P

P

T

L

M

P

V

I

P

S

H

Q

D

D

T

P

V

-

I

L

A

Y

Q

N

V

V

D

D

G

G

V

V

T

E

N

N

A

A

V

L

A

E

I

I

E

T

S

T

D

D

I

I

L

-

G

Q

E

S

L

I

L

L

M

I

V

R

G

G

P

P

G

G

A
|
A

G
|
G

G

P

D

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

L

L

G

S

D

D

I

L

active site: D196 (= D203), K200 (= K207)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ A10), G7 (= G11), Y8 (≠ L12), G9 (= G13), N10 (≠ T14), V11 (= V15), T37 (≠ E39), R38 (≠ I40), V72 (≠ L80), S73 (≠ M81), S74 (≠ G82), P82 (= P87), T100 (≠ A105), N101 (= N106), K102 (= K107), G127 (≠ A132), S131 (≠ G136), N155 (= N161), G156 (= G162), T157 (= T163), Y183 (= Y190), A184 (= A191), E185 (= E192), D191 (= D198), D196 (= D203), K200 (= K207), A281 (= A303), G282 (= G304), A286 (≠ T308)

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
30% identity, 71% coverage: 5:316/440 of query aligns to 3:308/319 of 4xb1A

query
sites
4xb1A

L

V

K

N

I

I

G

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

A

R

S

A

L

L

V

A

R

E

I

I

L

I

Q

A

D

E

R

K

H

S

E

R

T

I

L

F

A

G

T

-

C

-

G

-

R

-

A

-

I

V

E

E

I

L

T

N

A

V

V

I

T

S

A

I

R

T

D
|
D

R
|
R

S

S

K

-

D

-

R

-

G

-

V

-

D

-

L

-

A

-

G

G

I

T

T

I

W

W

F

G

D

D

D

F

A

D

V

L

-

L

-

E

-

A

-

K

-

E

-

V

-
x
K

-

E

-

S

-

T

-

G

S

K

L

L

A

S

S

N

Q

I

G

G

G

D

I

Y

D

E

V

V

F

Y

-

N

-

F

-

S

-

P

V

Q

E

E

L

L

M

V

G

E

-

E

-

V

-

K

-

P

-

N

-

I

-

L

-

V

-

D

-
x
V

G
x
S

T
x
S

G

W

D

D

P

E

A

A

Y

H

A

E

S

M

V

Y

K

K

A

V

A

A

L

L

T

G

H

E

G

G

V

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

A

N

H

Y

G

Y

I

D

E

E

L

L

A

M

E

N

I

L

A

A

E

K

K

E

H

N

G

N

A

A

L

G

L

I

N

F

Y

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

A

V

L

L

R

R

E

E

S

N

M

L

T

L

G

G

N

E

T

N

I

I

S

K

R

R

V

I

Y

D

G

A

I

V

M

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

E

-

K

S

E

E

G

G

L

K

S

T

F

L

E

D

A

D

C

A

L

I

K

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

A

S

F

K

D

D

I

I

E

D

G

G

N

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

S

K

I

I

L

L

T

H

S

W

L

V

A

S

F

Y

G

G

T

E

A

P

I

P

A

E

D

E

I

-

Y

-

L

-

E

-

G

-

I

-

T

-

N

R

I

L

S

G

I

I

E

R

D

E

I

V

Q

R

A

D

A

A

A

R

D

N

L

-

G

-

Y

-

R

-

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

Q

R

V

T

S

E

K

S

G

G

K

I

I

E

S

Q

-

R

-

V

V

H

K

P

P

T

R

M

K

V

L

P

S

H

S

D

D

T

N

V

P

I

L

A

L

Q

-

V

V

D

E

G

G

V

V

T

Q

N

N

A

A

V

A

A

V

I

I

E

R

S

T

D

N

I

N

L

L

G

G

E

E

L

V

L

I

M

L

V

K

G

G

P

P

G

G

A
x
G

G

G

D

R

A

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T
|
T

A

A

S

S

A

G

V

V

L

F

G

T

D

D

I

I

active site: D211 (= D203), K215 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A10), F10 (≠ L12), G11 (= G13), T12 (= T14), V13 (= V15), D39 (= D47), R40 (= R48), K57 (vs. gap), V91 (vs. gap), S92 (≠ G83), S93 (≠ T84), S114 (≠ A105), K116 (= K107), S141 (≠ A132), G295 (≠ A303), T300 (= T308)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
30% identity, 71% coverage: 5:316/440 of query aligns to 3:308/319 of O58802

query
sites
O58802

L

V

K

N

I

I

G

S

I

I

A

F

G

G

L
x
F

G

G

T
|
T

V
|
V

G

G

A

R

S

A

L

L

V

A

R

E

I

I

L

I

Q

A

D

E

R

K

H

S

E

R

T

I

L

F

A

G

T

-

C

-

G

-

R

-

A

-

I

V

E

E

I

L

T

N

A

V

V

I

T

S

A

I

R

T

D

D

R
|
R

S

S

K

-

D

-

R

-

G

-

V

-

D

-

L

-

A

-

G

G

I

T

T

I

W

W

F

G

D

D

D

F

A

D

V

L

-

L

-

E

-

A

-

K

-

E

-

V

-
x
K

-

E

-

S

-

T

-

G

S

K

L

L

A

S

S

N

Q

I

G

G

G

D

I

Y

D

E

V

V

F

Y

-

N

-

F

-

S

-

P

V

Q

E

E

L

L

M

V

G

E

-

E

-

V

-

K

-

P

-

N

-

I

-

L

-

V

-

D

-

V

G
x
S

T
x
S

G

W

D

D

P

E

A

A

Y

H

A

E

S

M

V

Y

K

K

A

V

A

A

L

L

T

G

H

E

G

G

V

I

H

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

A

N

H

Y

G

Y

I

D

E

E

L

L

A

M

E

N

I

L

A

A

E

K

K

E

H

N

G

N

A

A

L

G

L

I

N

F

Y

F

E
|
E

A

S

A

T

V
|
V

A

M

G
x
A

G

G

I
x
T

P

P

V

I

I

I

K

G

A

V

L

L

R

R

E

E

S

N

M

L

T

L

G

G

N

E

T

N

I

I

S

K

R

R

V

I

Y

D

G

A

I

V

M

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

E

-

K

S

E

E

G

G

L

K

S

T

F

L

E

D

A

D

C

A

L

I

K

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

A

S

F

K

D

D

I

I

E

D

G

G

N

I

D

D

T

A

A

Y

H

Y

K

K

L

A

S

K

I

I

L

L

T

H

S

W

L

V

A

S

F

Y

G

G

T

E

A

P

I

P

A

E

D

E

I

-

Y

-

L

-

E

-

G

-

I

-

T

-

N

R

I

L

S

G

I

I

E

R

D

E

I

V

Q

R

A

D

A

A

A

R

D

N

L

-

G

-

Y

-

R

-

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

Q

R

V

T

S

E

K

S

G

G

K

I

I

E

S

Q

-

R

-

V

V

H

K

P

P

T

R

M

K

V

L

P

S

H

S

D

D

T

N

V

P

I

L

A

L

Q

-

V

V

D

E

G

G

V

V

T

Q

N

N

A

A

V

A

A

V

I

I

E

R

S

T

D

N

I

N

L

L

G

G

E

E

L

V

L

I

M

L

V

K

G

G

P

P

G

G

A

G

G
|
G

G

G

D

R

A

V

T

T

A

A

S

S

A

G

V

V

L

F

G

T

D

D

I

I

F10 (≠ L12) binding NADPH
T12 (= T14) binding NADPH
V13 (= V15) binding NADPH
R40 (= R48) binding NADPH; mutation to A: Increases activity with NADP.
K57 (vs. gap) binding NADPH; mutation to A: Increases activity with NADP.
S92 (≠ G83) binding NADPH
S93 (≠ T84) binding NADPH
S114 (≠ A105) binding NADPH
K116 (= K107) binding NADPH
E140 (= E131) binding Na(+)
V143 (= V134) binding Na(+)
A145 (≠ G136) binding Na(+)
T147 (≠ I138) binding Na(+)
G296 (= G304) binding NADPH

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
30% identity, 71% coverage: 5:316/440 of query aligns to 3:308/319 of 4xb2A

query
sites
4xb2A

L

V

K

N

I

I

G

S

I

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

A

R

S

A

L

L

V

A

R

E

I

I

L

I

Q

A

D

E

R

K

H

S

E

R

T

I

L

F

A

G

T

-

C

-

G

-

R

-

A

-

I

V

E

E

I

L

T

N

A

V

V

I

T

S

A

I

R

T

D

D

R
|
R

S

S

K

-

D

-

R

-

G

-

V

-

D

-

L

-

A

-

G

G

I

T

T

I

W

W

F

G

D

D

D

F

A

D

V

L

-

L

-

E

-

A

-

K

-

E

-

V

-

A

-

E

-

S

-

T

-

G

S

K

L

L

A

S

S

N

Q

I

G

G

G

D

I

Y

D

E

V

V

F

Y

-

N

-

F

-

S

-

P

V

Q

E

E

L

L

M

V

G

E

-

E

-

V

-

K

-

P

-

N

-

I

-

L

-

V

-

D

-
x
V

G
x
S

T
x
S

G

W

D

D

P

E

A

A

Y

H

A

E

S

M

V

Y

K

K

A

V

A

A

L

L

T

G

H

E

G

G

V

I

H

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

A

N

H

Y

G

Y

I

D

E

E

L

L

A

M

E

N

I

L

A

A

E

K

K

E

H

N

G

N

A

A

L

G

L

I

N

F

Y

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

V

I

I

I

K

G

A

V

L

L

R

R

E

E

S

N

M

L

T

L

G

G

N

E

T

N

I

I

S

K

R

R

V

I

Y

D

G

A

I

V

M

V

N

N

G
x
A

T
x
S

C

T

N

T

Y

F

I

I

L

L

T

T

K

K

M

M

E

-

K

S

E

E

G

G

L

K

S

T

F

L

E

D

A

D

C

A

L

I

K

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

A

S

F

K

D
|
D

I

I

E

D

G

G

N

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

S

K

I

I

L

L

T

H

S

W

L

V

A

S

F

Y

G

G

T

E

A

P

I

P

A

E

D

E

I

-

Y

-

L

-

E

-

G

-

I

-

T

-

N

R

I

L

S

G

I

I

E

R

D

E

I

V

Q

R

A

D

A

A

A

R

D

N

L

-

G

-

Y

-

R

-

I

V

K

R

L

L

L

-

G

-

V

V

A

A

Q

Q

R

V

T

S

E

K

S

G

G

K

I

I

E

S

Q

-

R

-

V

V

H

K

P

P

T

R

M

K

V

L

P

S

H

S

D

D

T

N

V

P

I

L

A

L

Q

-

V

V

D

E

G

G

V

V

T

Q

N

N

A

A

V

A

A

V

I

I

E

R

S

T

D

N

I

N

L

L

G

G

E

E

L

V

L

I

M

L

V

K

G

G

P

P

G

G

A
x
G

G

G

D

R

A

V

T
|
T

A

A

S

S

A

G

V

V

L

F

G

T

D

D

I

I

active site: D211 (= D203), K215 (= K207)
binding l-homoserine: A171 (≠ G162), S172 (≠ T163), D206 (= D198), K215 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A10), F10 (≠ L12), G11 (= G13), T12 (= T14), V13 (= V15), R40 (= R48), V91 (vs. gap), S92 (≠ G83), S93 (≠ T84), S114 (≠ A105), N115 (= N106), K116 (= K107), S141 (≠ A132), G295 (≠ A303), T300 (= T308)

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
29% identity, 51% coverage: 5:230/440 of query aligns to 3:236/319 of 3ingA

query
sites
3ingA

L

I

K

R

I

I

G

I

I

L

A

M

G
|
G

L
x
T

G
|
G

T
x
N

V
|
V

G

G

A

L

S

N

L

V

V

L

R

R

I

I

L

I

Q

D

D

-

R

-

H

-

E

-

T

-

L

-

A

A

T

S

T

N

C

R

G

R

R

R

-

F

A

S

I

I

E

K

I

V

T

V

A

G

V

V

T

S

-
x
D

A
x
S

R

R

D

S

R

Y

S

A

K

S

D

G

R

R

G

N

V

L

D

D

L

I

A

S

G

S

I

I

T

I

W

S

F

N

D
x
K

D

E

A

K

V

T

S

G

L

R

A

I

S

S

Q

D

G

R

G

A

I

F

D

S

V

G

F

P

V

E

E

D

L

L

M

M

G

G

G

E

T

A

G

A

D

D

-

L

-

V

-

D

-
x
C

-
x
T

P
|
P

A

A

-

S

-

R

-

D

-

G

-

V

-

R

-

E

Y

Y

A

S

S

L

V

Y

K

R

A

M

A

A

L

F

T

E

H

S

G

G

V

M

H

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

A

N

H

K

G

W

I

H

E

D

L

I

A

M

E

D

I

S

A

A

E

N

K

Q

H

N

G

S

A

K

L

Y

L

I

N

R

Y

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

V

L

I

F

K

S

A

V

L

L

R

D

E

Y

S

S

M

I

T

L

G

P

N

S

T

K

I

V

S

K

R

R

V

F

Y

R

G

G

I

I

M

V

N

S

G

S

T

T

C

I

N

N

Y

Y

I

V

L

I

T

R

K

N

M

M

E

-

K

A

E

N

G

G

L

R

S

S

F

L

E

R

A

D

C

V

L

V

K

D

E

D

A

A

Q

I

R

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

A

Q

F

D

D

D

I

L

E

N

G

G

N

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

S

V

I

I

L

L

T

V

S

N

L

H

A

I

F

F

G

G

T

T

A

E

I

Y

A

T

A

L

D

N

D

D

I

V

Y

E

L

Y

E

S

G

G

I

V

active site: D209 (= D203), K213 (= K207)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G11), T10 (≠ L12), G11 (= G13), N12 (≠ T14), V13 (= V15), D38 (vs. gap), S39 (≠ A45), K57 (≠ D63), C85 (vs. gap), T86 (vs. gap), P87 (= P87), A112 (= A105), N113 (= N106), K114 (= K107), A139 (= A132), E198 (= E192), S199 (≠ A193)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 72% coverage: 5:320/440 of query aligns to 558:909/916 of O81852

query
sites
O81852

L

L

K

A

I

M

G

G

I

I

A

V

G

G

L

P

G

G

T

L

V

I

G

G

A

A

S

T

L

L

V

L

R

D

I

Q

L

L

Q

R

D

D

R

Q

H

A

E

A

T

V

L

L

A

K

T

Q

T

E

C

F

G

N

R

I

A

D

I

L

E

R

I

V

T

L

A

G

V

I

T

T

A

G

R

S

D

K

R

K

S

M

-

L

K

S

D

D

R

I

G

G

V

I

D

D

L

L

A

S

G

-

I

-

T

R

W

W

F

R

D

E

D

L

A

L

V

N

S

E

L

K

A

G

S

T

Q

E

G

A

G

D

I

L

D

D

V

K

F

F

V

T

E

Q

L

Q

M

V

G

H

G

G

-

N

-

H

-

F

-

I

-

P

-

N

-

S

-

V

-

D

-

C

T

T

G

A

D

D

P

S

A

A

Y

I

A

A

S

S

-

R

V

Y

K

Y

A

D

A

W

L

L

T

R

H

K

G

G

V

I

H

H

V

V

V

I

T

T

A

P

N

N

K

K

A

K

L

A

L

N

A

S

A

G

-

P

-

L

-

D

H

Q

G

Y

I

L

E

K

L

L

A

R

E

D

I

L

A

Q

E

R

K

K

H

S

G

Y

A

T

L

H

L

Y

N

F

Y

Y

E

E

A

A

A

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V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

T

L

L

R

R

E

G

S

L

M

L

-

E

T

T

G

G

N

D

T

K

I

I

S

L

R

R

V

I

Y

E

G

G

I

I

M

C

N

S

G

G

T

T

C

L

N

S

Y

Y

I

L

L

F

T

N

K

N

M

F

E

V

K

G

E

D

G

R

L

-

S

S

F

F

E

S

A

E

C

V

L

V

K

T

E

E

A

A

Q

K

R

N

L

A

G

G

Y

F

A

T

E

E

A

P

D

D

P

P

A

R

F

D

D

D

I

L

E

S

G

G

N

T

D

D

T

V

A

A

H

R

K

K

L

V

S

I

I

I

L

L

T

A

S

R

L

E

A

S

F

-

G

G

T

L

A

K

I

L

A

D

A

L

D

A

D

D

I

L

-

P

-

I

-

R

-

S

-

L

-

V

-

P

-

E

Y

P

L

L

E

K

G

G

I

C

T

T

N

S

I

V

S

E

-

E

-

F

I

M

E

E

D

K

I

L

-

P

Q

Q

A

Y

A

D

A

G

D

D

L

L

-

A

-

K

-

E

-

R

-

L

-

D

-

A

-

E

-

N

-

S

G

G

Y

E

R

V

I

L

K

R

L

Y

L

V

G

G

V

V

-

V

-

D

A

A

Q

V

R

N

T

Q

E

K

S

G

G

T

I

V

E

E

Q

L

R

R

V

R

H

Y

P

K

T

K

M

E

V

H

P

P

H

-

D

-

T

-

V

-

I

F

A

A

Q

Q

V

L

D

A

G

G

V

S

T

D

N

N

A

I

V

I

A

A

I

F

E

T

S

T

D

T

I

R

L

Y

G

K

E

D

-

H

-

P

L

L

L

I

M

V

V

R

G

G

P

P

G

G

A

A

G

G

G

A

D

Q

A

V

T

T

A

A

S

G

A

G

V

I

L

F

G

S

D

D

I

I

A

L

D

R

I

L

A

A

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

O94671 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
28% identity, 48% coverage: 5:215/440 of query aligns to 8:239/376 of O94671

query
sites
O94671

L

V

K

N

I

V

G

A

I

I

A

V

G

G

L

T

G

G

T

N

V

I

G

G

A

G

S

E

L

L

V

L

R

N

I

Q

L

I

Q

K

D

G

R

F

H

N

E

E

T

N

L

A

A

S

T

T

T

N

C

G

G

T

R

T

A

S

I

F

E

N

I

V

T

V

A

A

V

I

T

S

A

S

R

M

D

E

-

G

-

H

-

Y

R

V

S

S

K

K

D

D

-

Y

R

Q

G

P

V

I

D

N

L

L

A

S

G

-

I

-

T

E

W

W

F

K

D

N

D

-

A

-

V

L

S

T

L

S

A

Q

S

N

Q

Q

G

G

-

A

-

Y

G

S

I

L

D

D

V

A

F

L

V

V

E

D

L

F

M

L

G

A

G

K

T

S

G

P

D

L

P

P

A

A

Y

I

-

L

-

V

-

D

-

N

-

T

-

A

-

S

-

E

-

A

-

I

-

A

A

Q

S

S

V

Y

K

P

A

K

A

F

L

L

T

S

H

K

G

K

V

I

H

N

V

I

V

A

T

T

A

P

N

N

K

K

A

K

L

A

L

F

A

S

A

A

H

S

G

N

I

D

E

V

L

Y

A

Q

E

N

I

I

-

I

-

K

-

A

A

S

E

K

K

E

H

S

G

G

A

A

L

L

N

M

Y

H

E

E

A

A

A

S

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

T

L

L

R

K

E

E

S

L

M

I

-

A

T

T

G

G

N

D

T

E

I

I

S

I

R

K

V

I

Y

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

K

V

M

W

E

S

K

P

E

N

G

G

-

K

-

G

-

T

L

A

S
|
S

F

F

E

S

A

D

C

I

L

V

K

K

E

I

A

A

Q

K

R

Q

L

N

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

A

R

F

D

D

D

I

L

E

N

G

G

N

M

D

D

T

V

A

A

H

R

K

K

L

V

S

T

I

I

L

L

T

S

S

R

L

I

A

A

S201 (= S177) modified: Phosphoserine

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
28% identity, 53% coverage: 5:235/440 of query aligns to 4:244/321 of 3jsaA

query
sites
3jsaA

L

I

K

N

I

L

G

S

I

I

A

F

G
|
G

L
|
L

G
|
G

T
x
N

V
|
V

G

G

A

L

S

N

L

L

V

L

R

R

I

I

L

I

Q

R

D

S

R

F

H

N

E

E

-

E

-

N

-

R

-

L

-

G

-

L

-

K

-

F

-

N

-

V

-

F

-

V

-

A

-
x
D

T
x
S

L

L

A

H

T

S

T

Y

C

Y

G

N

R

E

A

R

I

I

E

D

I

I

T

G

A

K

V

V

T

I

A

S

R

Y

D

-

R

-

S

-

K

K

D

E

R

K

G

G

V

-

D

S

L

L

A

D

G

S

I

L

T

E

W

Y

F

E

D

S

D

I

A

S

V

A

S

S

L

E

A

A

S

L

Q

A

G

R

G

D

I

F

D

D

V

I

F

V

V

V

E

D

L
x
A

M
x
T

G
x
P

G

A

T

S

G

A

D

D

-

G

-

K

P

K

A

E

Y

L

A

A

S

F

V

Y

K

K

A

E

A

T

L

F

T

E

H

N

G

G

V

K

H

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

L

L

A

A

A

N

H

F

G

W

I

P

E

E

L

I

A

M

E

E

I

Y

A

A

E

R

K

S

H

N

G

N

A

R

L

R

L

I

N

R

Y

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

V

L

I

F

K

S

A

F

L

I

R

D

E

Y

S

S

M

V

T

L

G

P

N

S

T

R

I

I

S

K

R

K

V

F

Y

R

G

G

I

I

M

V

N

S

G

L

T

T

C

I

N

N

Y

Y

I

F

L

I

T

R

K

E

M

L

E

A

K

N

E

K

G

R

L

-

S

E

F

F

E

D

A

D

C

V

L

L

K

S

E

E

A

A

Q

T

R

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

A

-

F

-

D

D

I

L

E

T

G

G

N

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

S

V

I

I

L

L

T

C

S

N

L

H

A

L

F

Y

G

G

T

S

A

S

I

Y

A

R

A

L

D

S

D

D

I

V

Y

F

L

Y

E

E

G

G

I

I

T

L

N

D

I

R

S

S

I

F

active site: D212 (= D203), K216 (= K207)
binding nicotinamide-adenine-dinucleotide: G10 (= G11), L11 (= L12), G12 (= G13), N13 (≠ T14), V14 (= V15), D42 (vs. gap), S43 (≠ T29), A90 (≠ L80), T91 (≠ M81), P92 (≠ G82), A117 (= A105), N118 (= N106), A144 (= A132)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 308

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
30% identity, 26% coverage: 98:211/440 of query aligns to 107:226/358 of 1tveA

query
sites
1tveA

H

N

G

G

V

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

H

D

G

L

I

A

E

T

L

W

A

K

E

A

I

L

A

F

E

S

K

N

H

K

-

P

-

T

G

N

A

G

L

F

L

V

N

Y

Y

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

R

R

E

E

S

I

M

I

-

Q

T

T

G

G

N

D

T

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

M

F

N

S

G

G

T
|
T

C

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

-

S

-

T

-

S

E

Q

K

A

E

N

G

D

L

V

S

K

F

F

E

S

A

D

C

V

L

V

K

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

A

R

F

D

D
|
D

I

L

E

N

G

G

N

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

S

T

I

I

L

V

active site: D218 (= D203), K222 (= K207)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K107), T175 (= T163), D213 (= D198), D218 (= D203)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
30% identity, 26% coverage: 98:211/440 of query aligns to 107:226/358 of 1q7gA

query
sites
1q7gA

H

N

G

G

V

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

A

K

L

A

L

F

A

S

H

D

G

L

I

A

E

T

L

W

A

K

E

A

I

L

A

F

E

S

K

N

H

K

-

P

-

T

G

N

A

G

L

F

L

V

N

Y

Y

H

E

E

A
|
A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

R

R

E

E

S

I

M

I

-

Q

T

T

G

G

N

D

T

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

M

F

N
x
S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

-

S

-

T

-

S

E

Q

K

A

E

N

G

D

L

V

S

K

F

F

E

S

A

D

C

V

L

V

K

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

A

R

F

D

D

D

I

L

E

N

G

G

N

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

S

T

I

I

L

V

active site: D218 (= D203), K222 (= K207)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A105), K116 (= K107), A143 (= A132), S173 (≠ N161), K222 (= K207)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
30% identity, 26% coverage: 98:211/440 of query aligns to 107:226/358 of 1ebuD

query
sites
1ebuD

H

N

G

G

V

I

H

S

V

I

V

A

T

T

A

P

N

N

K
|
K

A

K

L

A

L

F

A

S

H

D

G

L

I

A

E

T

L

W

A

K

E

A

I

L

A

F

E

S

K

N

H

K

-

P

-

T

G

N

A

G

L

F

L

V

N

Y

Y

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

R

R

E

E

S

I

M

I

-

Q

T

T

G

G

N

D

T

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

-

S

-

T

-

S

E

Q

K

A

E

N

G

D

L

V

S

K

F

F

E

S

A

D

C

V

L

V

K

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

A

R

F

D

D

D

I

L

E

N

G

G

N

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

S

T

I

I

L

V

active site: D218 (= D203), K222 (= K207)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K107)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
30% identity, 26% coverage: 98:211/440 of query aligns to 107:226/358 of 1ebfA

query
sites
1ebfA

H

N

G

G

V

I

H

S

V

I

V

A

T

T

A
x
P

N

N

K

K

A

K

L

A

L

F

A

S

H

D

G

L

I

A

E

T

L

W

A

K

E

A

I

L

A

F

E

S

K

N

H

K

-

P

-

T

G

N

A

G

L

F

L

V

N

Y

Y

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

V

I

I

I

K

S

A

F

L

L

R

R

E

E

S

I

M

I

-

Q

T

T

G

G

N

D

T

E

I

V

S

E

R

K

V

I

Y

E

G

G

I

I

M

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

K

E

M

F

-

S

-

T

-

S

E

Q

K

A

E

N

G

D

L

V

S

K

F

F

E

S

A

D

C

V

L

V

K

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

A

R

F

D

D

D

I

L

E

N

G

G

N

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

S

T

I

I

L

V

active site: D218 (= D203), K222 (= K207)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A105)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

Query Sequence

>WP_011975608.1 NCBI__GCF_000017145.1:WP_011975608.1
MADALKIGIAGLGTVGASLVRILQDRHETLATTCGRAIEITAVTARDRSKDRGVDLAGIT
WFDDAVSLASQGGIDVFVELMGGTGDPAYASVKAALTHGVHVVTANKALLAAHGIELAEI
AEKHGALLNYEAAVAGGIPVIKALRESMTGNTISRVYGIMNGTCNYILTKMEKEGLSFEA
CLKEAQRLGYAEADPAFDIEGNDTAHKLSILTSLAFGTAIAADDIYLEGITNISIEDIQA
AADLGYRIKLLGVAQRTESGIEQRVHPTMVPHDTVIAQVDGVTNAVAIESDILGELLMVG
PGAGGDATASAVLGDIADIAKSRPGAQHVPAFGRPANALLPYKRARMQSHEGGYFIRLKV
VDRTGVFANVAKQMADNDISLESIMQHSKHYADPAEPKTIILVTHATFEASVRKAIVSIK
AEGYLVGEPQVIRIERPKAW

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory