SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011976095.1 NCBI__GCF_000017145.1:WP_011976095.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

C1DFH7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Azotobacter vinelandii (strain DJ / ATCC BAA-1303) (see paper)
64% identity, 100% coverage: 1:339/339 of query aligns to 1:338/338 of C1DFH7

query
sites
C1DFH7

M

M

R

K

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

S

L

I

I

I

K

Q

S

S

K

K

V

V

A

A

I

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

R

H

A

A

H

H

A

A

L

C

N

N

L

L

K

K

D

D

S

S

G

G

A

V

Q

-

N

D

V

V

A

Y

I

V

A

G

L

L

K

R

S

A

G

G

S

S

A

A

T

S

A

V

K

A

K

K

A

A

E

E

A

A

D

H

G

G

F

L

K

T

V

V

M

K

T

S

V

V

A

K

E

D

A

A

A

V

A

A

W

A

A

A

D

D

L

V

M

V

M

M

M

I

A

L

T

T

P

P

D

D

E

E

L

F

Q

Q

A

G

D

R

I

L

Y

Y

K

K

A

D

E

E

I

I

A

E

G

P

N

N

I

L

R

K

D

K

G

G

A

A

A

T

I

L

A

A

F

F

A

A

H

H

G

G

L

F

N

S

V

I

H

H

F

Y

G

N

L

Q

I

V

E

V

P

P

K

R

A

A

S

D

V

L

D

D

V

V

V

I

M

M

I

I

A

A

P

P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

S

E

E

Y

F

Q

V

K

R

G

G

V

I

P

P

C

D

L

L

V

I

A

A

V

V

H

Y

Q

Q

D

D

A

A

S

S

G

G

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

C

G

G

V

V

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

D

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E

E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

C

V

V

E

E

L

L

I

V

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

A

P

P

E

E

M

M

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E
|
E

V

L

K

K

L

L

I

I

V

V

D

D

L

L

I

M

Y

F

E

E

G

G

I

I

A

A

N

N

M

M

N

N

Y

Y

S

S

I

I

S

S

N

N

T

N

A

A

E

E

W

Y

G

G

E

E

Y

Y

V

V

T

T

G

G

P

P

R

E

I

V

I

I

T

N

E

E

E

Q

T

S

K

R

A

Q

E

A

M

M

K

R

R

N

V

A

L

L

K

K

D

R

I

I

Q

Q

T

D

G

G

K

E

F

Y

T

A

S

K

E

M

W

F

M

I

Q

T

E

E

Y

G

R

A

S

A

G

N

A

Y

A

P

R

S

F

M

K

T

G

A

I

Y

R

R

V

N

N

N

D

A

A

A

H

H

Q

Q

I

I

E

E

E

V

V

V

G

G

A

E

K

K

L

L

R

R

G

T

M

M

P

P

W

W

I

I

G

A

K

A

N

N

K

K

L

I

V

V

D

D

K

K

A

T

K

K

N

N

D190 (= D191) binding Mg(2+)
E226 (= E227) binding Mg(2+)
E230 (= E231) binding Mg(2+)

8upqB Campylobacter jejuni ketol-acid reductoisomerase in complex with 2,3- dihydroxy-3-isovalerate.
65% identity, 100% coverage: 1:339/339 of query aligns to 2:339/342 of 8upqB

query
sites
8upqB

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

N

L

L

I

I

K

K

S

S

K

K

V

V

A

A

I

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

R

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

A

V

Q

-

N

N

V

V

A

T

I

I

A

G

L

L

K

R

S

E

G

G

S

S

A

V

T

S

A

A

K

V

K

K

A

A

E

K

A

N

D

A

G

G

F

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

M

I

M

M

M

I

A

L

T

A

P

P

D

D

E

E

L

I

Q

Q

A

A

D

D

I

I

Y

F

K

N

A

V

E

E

I

I

A

K

G

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

I

I

A

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

A

K

S

G

V

V

D

D

V

V

V

I

M

M

I

I

A

A

P

P

K

K

G
x
A

P
|
P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

S

S

G

K

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

S

G

A

V

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E
|
E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S
|
S

N

N

T

T

A
|
A

E

E

W

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

K

K

D

D

I

I

Q

Q

T

N

G

G

K

V

F

F

T

A

S

K

E

D

W

F

M

I

Q

L

E

E

Y

R

R

R

S

A

G

G

A

F

A

A

R

R

F

M

K

H

G

A

I

E

R

R

R

K

V

N

N

M

D

N

A

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

K

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

G

S

K

A

N

K

K

K

L

L

V

V

D

D

K

A

A

D

K

K

N

N

binding magnesium ion: D191 (= D191), E195 (= E195)
binding (2R)-2,3-dihydroxy-3-methylbutanoic acid: A132 (≠ G132), P133 (= P133), D191 (= D191), E195 (= E195), E231 (= E231), S252 (= S252), A255 (= A255)

8uppA Campylobacter jejuni ketol-acid reductoisomerase in complex with NADPH and hoe704
65% identity, 97% coverage: 1:328/339 of query aligns to 1:327/327 of 8uppA

query
sites
8uppA

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

N

L

L

I

I

K

K

S

S

K

K

V

V

A

A

I

I

G

G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

R

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

A

V

Q

-

N

N

V

V

A

T

I

I

A

G

L

L

K
x
R

S

E

G

G

S
|
S

A

V

T

S

A

A

K

V

K

K

A

A

E

K

A

N

D

A

G

G

F

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

M

I

M

M

M

I

A
x
L

T

A

P
|
P

D
|
D

E

E

L
x
I

Q
|
Q

A

A

D

D

I
|
I

Y

F

K

N

A

V

E

E

I

I

A

K

G

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

I

I

A

A

F

F

A

A

H
|
H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

A

K

S

G

V

V

D

D

V

V

V

I

M

M

I

I

A

A

P

P

K

K

G
x
A

P
|
P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

S

S

G

K

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

S

G

A

V

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E
|
E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I
|
I

S
|
S

N

N

T

T

A
|
A

E

E

W

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

K

K

D

D

I

I

Q

Q

T

N

G

G

K

V

F

F

T

A

S

K

E

D

W

F

M

I

Q

L

E

E

Y

R

R

R

S

A

G

G

A

F

A

A

R

R

F

M

K

H

G

A

I

E

R

R

R

K

V

N

N

M

D

N

A

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

K

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

binding magnesium ion: D190 (= D191), E194 (= E195)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F24 (≠ Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (≠ K48), S50 (= S51), L79 (≠ A80), P81 (= P82), D82 (= D83), I84 (≠ L85), Q85 (= Q86), I88 (= I89), H107 (= H108), P132 (= P133), I250 (= I251), S251 (= S252)
binding (2R)-(dimethylphosphoryl)(hydroxy)acetic acid: A131 (≠ G132), P132 (= P133), D190 (= D191), E194 (= E195), E230 (= E231), I250 (= I251), S251 (= S252), A254 (= A255)

8sxdA Campylobacter jejuni keto-acid reductoisomerase in complex with intermediate and NADP+
65% identity, 97% coverage: 1:328/339 of query aligns to 1:327/327 of 8sxdA

query
sites
8sxdA

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

N

L

L

I

I

K

K

S

S

K

K

V

V

A

A

I

I

G
|
G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

R

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

A

V

Q

-

N

N

V

V

A

T

I

I

A

G

L

L

K

R

S

E

G

G

S
|
S

A

V

T
x
S

A

A

K

V

K

K

A

A

E

K

A

N

D

A

G

G

F

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

M

I

M

M

M

I

A
x
L

T
x
A

P
|
P

D
|
D

E

E

L

I

Q

Q

A

A

D

D

I

I

Y

F

K

N

A

V

E

E

I

I

A

K

G

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

I

I

A

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

A

K

S

G

V

V

D

D

V

V

V

I

M

M

I

I

A

A

P
|
P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

S

S

G

K

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

S

G

A

V

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

W

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

K

K

D

D

I

I

Q

Q

T

N

G

G

K

V

F

F

T

A

S

K

E

D

W

F

M

I

Q

L

E

E

Y

R

R

R

S

A

G

G

A

F

A

A

R

R

F

M

K

H

G

A

I

E

R

R

R

K

V

N

N

M

D

N

A

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

K

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

binding magnesium ion: D190 (= D191), E194 (= E195)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G23 (= G23), F24 (≠ Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), S50 (= S51), S52 (≠ T53), L79 (≠ A80), A80 (≠ T81), P81 (= P82), D82 (= D83), P129 (= P130)
binding 3-hydroxy-3-methyl-2-oxobutanoic acid: D190 (= D191), E194 (= E195)

7rduA Crystal structure of campylobacter jejuni keto said reductoisomerase in complex with magnesium and oxidixized and reduced NADPH
65% identity, 97% coverage: 1:328/339 of query aligns to 2:328/329 of 7rduA

query
sites
7rduA

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

N

L

L

I

I

K

K

S

S

K

K

V

V

A

A

I

I

G
|
G

Y
x
F

G
|
G

S
|
S

Q
|
Q

G

G

R

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

A

V

Q

-

N

N

V

V

A

T

I

I

A

G

L

L

K
x
R

S

E

G

G

S
|
S

A

V

T
x
S

A

A

K

V

K

K

A

A

E

K

A

N

D

A

G

G

F

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

M

I

M

M

M

I

A
x
L

T
x
A

P
|
P

D
|
D

E

E

L

I

Q

Q

A

A

D

D

I
|
I

Y

F

K

N

A

V

E

E

I

I

A

K

G

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

I

I

A

A

F

F

A
|
A

H
|
H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

A

K

S

G

V

V

D

D

V

V

V

I

M

M

I

I

A

A

P
|
P

K
|
K

G
x
A

P

P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

S

S

G

K

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

S

G

A

V

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

A

E

E

C

T

E

E

T

T

D

D

L

L

F

F

G

G

E

E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

W

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

K

K

D

D

I

I

Q

Q

T

N

G

G

K

V

F

F

T

A

S

K

E

D

W

F

M

I

Q

L

E

E

Y

R

R

R

S

A

G

G

A

F

A

A

R

R

F

M

K

H

G

A

I

E

R

R

R

K

V

N

N

M

D

N

A

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

K

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), G26 (= G25), S27 (= S26), Q28 (= Q27), R48 (≠ K48), S51 (= S51), S53 (≠ T53), A81 (≠ T81), P82 (= P82), D83 (= D83), I89 (= I89), A107 (= A107), H108 (= H108), P130 (= P130), K131 (= K131), A132 (≠ G132)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G24 (= G23), F25 (≠ Y24), G26 (= G25), S27 (= S26), Q28 (= Q27), S51 (= S51), S53 (≠ T53), L80 (≠ A80), P82 (= P82), D83 (= D83), I89 (= I89), A107 (= A107), H108 (= H108)

4xiyA Crystal structure of ketol-acid reductoisomerase from azotobacter (see paper)
64% identity, 97% coverage: 1:328/339 of query aligns to 1:327/328 of 4xiyA

query
sites
4xiyA

M

M

R

K

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

S

L

I

I

I

K

Q

S

S

K

K

V

V

A

A

I

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

R

H

A

A

H

H

A

A

L

C

N

N

L

L

K

K

D

D

S

S

G

G

A

V

Q

-

N

D

V

V

A

Y

I

V

A

G

L

L

K

R

S

A

G

G

S

S

A

A

T

S

A

V

K

A

K

K

A

A

E

E

A

A

D

H

G

G

F

L

K

T

V

V

M

K

T

S

V

V

A

K

E

D

A

A

A

V

A

A

W

A

A

A

D

D

L

V

M

V

M

M

M

I

A

L

T

T

P

P

D

D

E

E

L

F

Q

Q

A

G

D

R

I

L

Y

Y

K

K

A

D

E

E

I

I

A

E

G

P

N

N

I

L

R

K

D

K

G

G

A

A

A

T

I

L

A

A

F

F

A

A

H

H

G

G

L

F

N

S

V

I

H

H

F

Y

G

N

L

Q

I

V

E

V

P

P

K

R

A

A

S

D

V

L

D

D

V

V

V

I

M

M

I

I

A

A

P

P

K
|
K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

S

E

E

Y

F

Q

V

K

R

G

G

V

I

P

P

C

D

L

L

V

I

A

A

V

V

H

Y

Q

Q

D

D

A

A

S

S

G

G

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

C

G

G

V

V

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

D

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

C

V

V

E

E

L

L

I

V

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

A

P

P

E

E

M

M

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E
|
E

V

L

K

K

L

L

I

I

V

V

D

D

L

L

I

M

Y

F

E

E

G

G

I

I

A

A

N

N

M

M

N

N

Y

Y

S

S

I

I

S

S

N

N

T

N

A

A

E

E

W

Y

G

G

E

E

Y

Y

V

V

T

T

G

G

P

P

R

E

I

V

I

I

T

N

E

E

E

Q

T

S

K

R

A

Q

E

A

M

M

K

R

R

N

V

A

L

L

K

K

D

R

I

I

Q

Q

T

D

G

G

K

E

F

Y

T

A

S

K

E

M

W

F

M

I

Q

T

E

E

Y

G

R

A

S

A

G

N

A

Y

A

P

R

S

F

M

K

T

G

A

I

Y

R

R

V

N

N

N

D

A

A

A

H

H

Q

Q

I

I

E

E

E

V

V

V

G

G

A

E

K

K

L

L

R

R

G

T

M

M

P

P

W

W

I

I

active site: K130 (= K131), D190 (= D191), E194 (= E195)
binding fe (iii) ion: D190 (= D191), E194 (= E195)
binding magnesium ion: E226 (= E227), E230 (= E231)

8swmA Crystal structure of campylobacter jejuni ketol-acid reductoisomerase in complex with 2-acetolactate
65% identity, 96% coverage: 4:327/339 of query aligns to 1:323/323 of 8swmA

query
sites
8swmA

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

N

L

L

I

I

K

K

S

S

K

K

V

V

A

A

I

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

R

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

A

V

Q

-

N

N

V

V

A

T

I

I

A

G

L

L

K

R

S

E

G

G

S

S

A

V

T

S

A

A

K

V

K

K

A

A

E

K

A

N

D

A

G

G

F

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

M

I

M

M

M

I

A

L

T

A

P

P

D

D

E

E

L

I

Q

Q

A

A

D

D

I

I

Y

F

K

N

A

V

E

E

I

I

A

K

G

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

I

I

A

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

A

K

S

G

V

V

D

D

V

V

V

I

M

M

I

I

A

A

P

P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

F

Q

T

K

L

G

G

V

T

P

P

C

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

S

S

G

K

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

S

G

A

V

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

W

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

K

K

D

D

I

I

Q

Q

T

N

G

G

K

V

F

F

T

A

S

K

E

D

W

F

M

I

Q

L

E

E

Y

R

R

R

S

A

G

G

A

F

A

A

R

R

F

M

K

H

G

A

I

E

R

R

R

K

V

N

N

M

D

N

A

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

K

N

L

L

R

R

G

A

M

M

P

P

W

W

binding magnesium ion: D187 (= D191), E191 (= E195)
binding (2S)-2-hydroxy-2-methyl-3-oxobutanoic acid: D187 (= D191), E191 (= E195)

8cy8A Apo form cryo-em structure of campylobacter jejune ketol-acid reductoisommerase crosslinked by glutaraldehyde
63% identity, 97% coverage: 1:328/339 of query aligns to 1:311/312 of 8cy8A

query
sites
8cy8A

M

I

R

T

V

V

Y

Y

D

D

R

K

D

D

A

C

D

D

L

L

N

N

L

L

I

I

K

K

S

S

K

K

V

V

A

A

I

I

G

G

Y

F

G

G

S

S

Q

Q

G

G

R

H

A

A

H

H

A

A

L

M

N

N

L

L

K

R

D

D

S

N

G

G

A

V

Q

-

N

N

V

V

A

T

I

I

A

G

L

L

K

R

S

N

G

A

S

-

A

-

T

-

A

-

K

-

A

-

E

-

A

-

D

-

G

G

F

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

S

A

K

W

I

A

A

D

D

L

V

M

I

M

M

M

I

A

L

T

A

P

P

D

D

E

E

L

I

Q

Q

A

A

D

D

I

I

Y

F

K

N

A

V

E

E

I

I

A

K

G

P

N

N

I

L

R

S

D

E

G

G

A

K

A

A

I

I

A

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

Y

G

G

L

Q

I

I

E

V

P

V

K

P

A

K

S

G

V

V

D

D

V

V

V

I

M

M

I

I

A

A

P

P

K

K

G

A

P

P

G

G

H

H

T

T

V

V

R

R

G

N

E

E

Y

-

Q

-

K

-

G

-

V

T

P

P

C

C

L

L

V

I

A

A

V

I

H

H

Q

Q

D

D

A

E

S

S

G

K

N

N

A

A

L

K

D

N

L

L

A

A

L

L

S

S

Y

Y

A

A

C

S

G

A

V

I

G

G

R

R

S

T

G

G

I

I

E

E

T

T

N

T

F

F

K

K

E

A

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L
|
L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

Q

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

E

P

P

E

E

M

M

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

Q

G

G

I

I

A

A

N

D

M

M

N

R

Y

Y

S

S

I

I

S

S

N

N

T

T

A

A

E

E

W

Y

G

G

E

D

Y

Y

V

I

T

T

G

G

P

P

R

K

I

I

T

T

E

E

T

T

K

K

A

K

E

A

M

M

K

K

R

G

V

V

L

L

K

K

D

D

I

I

Q

Q

T

N

G

G

K

V

F

F

T

A

S

K

E

D

W

F

M

I

Q

L

E

E

Y

R

R

R

S

A

G

G

A

F

A

A

R

R

F

M

K

H

G

A

I

E

R

R

R

K

V

N

N

M

D

N

A

D

H

S

Q

L

I

I

E

E

E

K

V

T

G

G

A

R

K

N

L

L

R

R

G

A

M

M

P

P

W

W

I

I

binding pentanedial: D174 (= D191), E178 (= E195), L182 (= L199)

C8WR67 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Alicyclobacillus acidocaldarius subsp. acidocaldarius (strain ATCC 27009 / DSM 446 / BCRC 14685 / JCM 5260 / KCTC 1825 / NBRC 15652 / NCIMB 11725 / NRRL B-14509 / 104-IA) (Bacillus acidocaldarius) (see paper)
60% identity, 96% coverage: 2:328/339 of query aligns to 3:327/344 of C8WR67

query
sites
C8WR67

R

K

V

I

Y

Y

D

D

R

A

D

D

A

I

D

S

L

I

N

Q

L

P

I

L

K

A

S

D

K

K

K

R

V

I

A

A

I

V

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

R

H

A

A

H

H

A

A

L

Q

N

N

L

L

K

R

D

D

S

S

G

G

A

F

Q

-

N

D

V

V

A

V

I

I

A

G

L

L

K
x
R

S

P

G

G

S

S

A
x
S

T

W

A

A

K

K

K

-

A

A

E

E

A

A

D

D

G

G

F

F

K

R

V

V

M

M

T

A

V

V

A

G

E

E

A

A

A

V

A

E

W

E

A

S

D

D

L

V

M

I

M

M

M

I

A

L

T

L

P

P

D
|
D

E
|
E

L
x
R

Q
|
Q

A

P

D

A

I

V

Y

Y

K

E

A

R

E

E

I

I

A

R

G

P

N

Y

I

L

R

T

D

A

G

G

A

K

A

A

I

L

A

A

F

F

A

A

H

H

G

G

L

F

N

N

V

I

H

H

F

F

G

S

L

Q

I

I

E

Q

P

P

K

P

A

K

S

D

V

V

D

D

V

V

V

F

M

M

I

V

A

A

P

P

K

K

G

G

P

P

G
|
G

H

H

T

L

V

V

R

R

G

R

E

V

Y

Y

Q

E

K

A

G

G

V

V

P

P

C

A

L

L

V

I

A

A

V

V

H

H

Q

Q

D

D

A

A

S

S

G

G

N

Q

A

A

L

K

D

D

L

L

A

A

L

L

S

A

Y

Y

A

A

C

R

G

G

V

I

G

G

G

A

G

G

R

R

S

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

K

R

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E
|
E

C

C

L

L

H

H

E

E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

N

R

Y

Y

S

S

I

I

S

S

N

D

T

T

A

A

E

Q

W

W

G

G

E

D

Y

F

V

T

T

S

G

G

P

P

R

R

I

I

T

N

E

E

T

T

K

K

A

K

E

E

M

M

K

R

R

R

V

I

L

L

K

A

D

D

I

I

Q

Q

T

S

G

G

K

A

F

F

T

A

S

K

E

S

W

W

M

I

Q

L

E

E

Y

N

R

Q

S

A

G

N

A

R

A

P

R

M

F

F

K

N

G

A

I

I

R

N

R

R

V

R

N

E

D

L

A

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

R

K

K

L

L

R

R

G

S

M

M

P

P

W

F

I

I

YGSQ 25:28 (= YGSQ 24:27) binding NADP(+)
R48 (≠ K48) binding NADP(+); mutation to P: Inversion of the cofactor specificity from NADPH to NADH.
S52 (≠ A52) binding NADP(+); mutation to D: Inversion of the cofactor specificity from NADPH to NADH.
DERQ 82:85 (≠ DELQ 83:86) binding NADP(+)
G133 (= G134) binding NADP(+)
D190 (= D191) binding Mg(2+)
E194 (= E195) binding Mg(2+)
E226 (= E227) binding Mg(2+)

4tskA Ketol-acid reductoisomerase from alicyclobacillus acidocaldarius (see paper)
60% identity, 96% coverage: 2:328/339 of query aligns to 2:326/333 of 4tskA

query
sites
4tskA

R

K

V

I

Y

Y

D

D

R

A

D

D

A

I

D

S

L

I

N

Q

L

P

I

L

K

A

S

D

K

K

K

R

V

I

A

A

I

V

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

R

H

A

A

H

H

A

A

L

Q

N

N

L

L

K

R

D

D

S

S

G

G

A

F

Q

-

N

D

V

V

A

V

I

I

A

G

L
|
L

K
x
R

S

P

G

G

S

S

A
x
S

T

W

A

A

K

K

K

-

A

A

E

E

A

A

D

D

G

G

F

F

K

R

V

V

M

M

T

A

V

V

A

G

E

E

A

A

A

V

A

E

W

E

A

S

D

D

L

V

M

I

M

M

M

I

A
x
L

T
x
L

P
|
P

D
|
D

E

E

L

R

Q

Q

A

P

D

A

I

V

Y

Y

K

E

A

R

E

E

I

I

A

R

G

P

N

Y

I

L

R

T

D

A

G

G

A

K

A

A

I

L

A

A

F

F

A

A

H
|
H

G

G

L

F

N

N

V

I

H

H

F

F

G

S

L

Q

I

I

E

Q

P

P

K

P

A

K

S

D

V

V

D

D

V

V

V

F

M

M

I

V

A

A

P

P

K
|
K

G

G

P
|
P

G

G

H

H

T

L

V

V

R

R

G

R

E

V

Y

Y

Q

E

K

A

G

G

V

V

P

P

C

A

L

L

V

I

A

A

V

V

H

H

Q

Q

D

D

A

A

S

S

G

G

N

Q

A

A

L

K

D

D

L

L

A

A

L

L

S

A

Y

Y

A

A

C

R

G

G

V

I

G

G

G

A

G

G

R

R

S

A

G

G

I

I

I

L

E

T

T

T

N

T

F

F

K

R

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

L

V

S

E

A

L

L

I

I

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

V

E

E

A

A

G

G

Y

Y

A

Q

P

P

E

E

M

I

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

V

M

K

K

L

L

I

I

V

V

D

D

L

L

I

I

Y

Y

E

E

G

G

I

L

A

E

N

Y

M

M

N
x
R

Y
|
Y

S

S

I
|
I

S

S

N
x
D

T

T

A

A

E
x
Q

W

W

G

G

E

D

Y

F

V

T

T

S

G

G

P

P

R

R

I

I

T

N

E

E

T

T

K

K

A

K

E

E

M

M

K

R

R

R

V

I

L

L

K

A

D

D

I

I

Q

Q

T

S

G

G

K

A

F

F

T

A

S

K

E

S

W

W

M

I

Q

L

E

E

Y

N

R

Q

S

A

G

N

A

R

A

P

R

M

F

F

K

N

G

A

I

I

R

N

R

R

V

R

N

E

D

L

A

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

R

K

K

L

L

R

R

G

S

M

M

P

P

W

F

I

I

active site: K129 (= K131), D189 (= D191), E193 (= E195)
binding magnesium ion: D189 (= D191), D189 (= D191), E193 (= E195), E193 (= E195), R246 (≠ N248), Y247 (= Y249), I249 (= I251), D251 (≠ N253), Q254 (≠ E256)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), L46 (= L47), R47 (≠ K48), S51 (≠ A52), L78 (≠ A80), L79 (≠ T81), P80 (= P82), D81 (= D83), H106 (= H108), P131 (= P133)

8ep7C Crystal structure of the ketol-acid reductoisomerase from bacillus anthracis in complex with NADP
57% identity, 97% coverage: 1:328/339 of query aligns to 1:326/328 of 8ep7C

query
sites
8ep7C

M

M

R

K

V

T

Y

Y

D

E

R

Q

D

D

A

A

D

N

L

V

N

G

L

L

I

L

K

Q

S

G

K

K

K

T

V

V

A

A

I

V

I

I

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

R

H

A

A

H

Q

A

A

L

Q

N

N

L

L

K

R

D

D

S

S

G

G

A

V

Q

E

N

-

V

V

A

V

I

V

A

G

L

V

K
x
R

S

P

G

G

S

K

A

-

T
x
S

A

F

K

E

K

V

A

A

E

K

A

A

D

D

G

G

F

F

K

E

V

V

M

M

T

S

V

V

A

S

E

E

A

A

A

V

A

R

W

T

A

A

D

Q

L

V

M

V

M

Q

M

M

A
x
L

T
x
L

P
|
P

D
|
D

E

E

L
x
Q

Q

Q

A

A

D

H

I
x
V

Y

Y

K

K

A

A

E

E

I

V

A

E

G

E

N

N

I

L

R

R

D

E

G

G

A

Q

A

M

I

L

A

L

F

F

A

S

H
|
H

G

G

L

F

N

N

V

I

H

H

F

F

G

G

L

Q

I

I

E

N

P

P

K

P

A

S

S

Y

V

V

D

D

V

V

V

A

M

M

I

V

A

A

P

P

K

K

G

S

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

V

Y

F

Q

Q

K

E

G

G

G

N

G

G

V

V

P

P

C

A

L

L

V

V

A

A

V

V

H

H

Q

Q

D

D

A

A

S

T

G

G

N

T

A

A

L

L

D

H

L

V

A

A

L

L

S

A

Y

Y

A

A

C

K

G

G

V

V

G

G

G

C

G

T

R

R

S

A

G

G

I

V

I

I

E

E

T

T

N

T

F

F

K

Q

E

E

C

T

E

E

T

T

D

D

L

L

F

F

G

G

E

E

Q

Q

V

A

V

V

L

L

C

C

G

G

L

V

V

T

E

A

L

L

I

V

R

K

A

A

G

G

F

F

E

E

T

T

L

L

V

T

E

E

A

G

G

G

Y

Y

A

R

P

P

E

E

M

I

A

A

Y

Y

F

F

E

E

C

C

L

L

H

H

E

E

V

L

K

K

L

L

I

I

V

V

D

D

L

L

I

M

Y

Y

E

E

G

G

I

L

A

T

N

N

M

M

N

R

Y

H

S

S

I

I

S

S

N

D

T

T

A

A

E

E

W

F

G

G

E

D

Y

Y

V

V

T

T

G

G

P

S

R

R

I

I

I

V

T

T

E

D

E

E

T

T

K

K

A

K

E

E

M

M

K

K

R

R

V

V

L

L

K

T

D

E

I

I

Q

Q

T

Q

G

G

K

E

F

F

T

A

S

K

E

K

W

W

M

I

Q

L

E

E

Y

N

R

Q

S

A

G

G

A

R

A

P

R

T

F

Y

K

N

G

A

I

M

R

K

V

A

N

E

D

Q

A

N

H

H

Q

Q

I

L

E

E

E

K

V

V

G

G

A

E

K

E

L

L

R

R

G

E

M

M

P

S

W

W

I

I

binding nadp nicotinamide-adenine-dinucleotide phosphate: Y24 (= Y24), G25 (= G25), S26 (= S26), Q27 (= Q27), R47 (≠ K48), S51 (≠ T53), L78 (≠ A80), L79 (≠ T81), P80 (= P82), D81 (= D83), Q83 (≠ L85), V87 (≠ I89), H106 (= H108)

P9WKJ7 Ketol-acid reductoisomerase (NADP(+)); KARI; Acetohydroxy-acid isomeroreductase; AHIR; Alpha-keto-beta-hydroxylacyl reductoisomerase; Ketol-acid reductoisomerase type 1; Ketol-acid reductoisomerase type I; EC 1.1.1.86 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
55% identity, 97% coverage: 1:330/339 of query aligns to 3:331/337 of P9WKJ7

query
sites
P9WKJ7

M

L

R

E

V

M

Y

F

Y

Y

D

D

R

D

D

D

A

A

D

D

L

L

N

S

L

I

I

I

K

Q

S

G

K

R

K

K

V

V

A

G

I

V

I

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

R

H

A

A

H

H

A

S

L

L

N

S

L

L

K

R

D

D

S

S

G

G

A

V

Q

Q

N

-

V

V

A

R

I

V

A

G

L

L

K

K

S

Q

G

G

S

S

A

R

T

S

A

R

K

P

K

K

A

V

E

E

A

E

D

Q

G

G

F

L

K

D

V

V

M

D

T

T

V

P

A

A

E

E

A

V

A

A

A

K

W

W

A

A

D

D

L

V

M

V

M

M

M

V

A

L

T

A

P

P

D

D

E

T

L

A

Q

Q

A

A

D

E

I

I

Y

F

K

A

A

G

E

D

I

I

A

E

G

P

N

N

I

L

R

K

D

P

G

G

A

D

A

A

I

L

A

F

F

F

A

G

H

H

G

G

L

L

N

N

V

V

H

H

F

F

G

G

L

L

I

I

E

K

P

P

K

P

A

A

S

D

V

V

D

A

V

V

V

A

M

M

I

V

A

A

P

P

K

K

G

G

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

Q

Y

F

Q

V

K

D

G

G

G

K

G

G

V

V

P

P

C

C

L

L

V

V

A

A

V

V

H

E

Q

Q

D

D

A

P

S

R

G

G

N

D

A

G

L

L

D

A

L

L

A

A

L

L

S

S

Y

Y

A

A

C

K

G

A

V

I

G

G

G

T

R

R

S

A

G

G

I

V

I

I

E

K

T

T

N

T

F

F

K

K

E

D

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

T

V

V

L

L

C

C

G

G

L

T

V

E

E

E

L

L

I

V

R

K

A

A

G

G

F

F

E

E

T

V

L

M

V

V

E

E

A

A

G

G

Y

Y

A

P

P

A

E

E

M

L

A

A

Y

Y

F

F

E
|
E

C

V

L

L

H

H

E
|
E

V

L

K

K

L

L

I

I

V

V

D

D

L

L

I

M

Y

Y

E

E

G

G

I

L

A

A

N

R

M

M

N

Y

Y

Y

S

S

I

V

S

S

N

D

T

T

A

A

E

E

W

F

G

G

E

G

Y

Y

V

L

T

S

G

G

P

P

R

R

I

V

I

I

T

D

E

A

E

G

T

T

K

K

A

E

E

R

M

M

K

R

R

D

V

I

L

L

K

R

D

E

I

I

Q

Q

T

D

G

G

K

S

F

F

T

V

S

H

E

K

W

L

M

V

Q

A

E

D

Y

V

R

E

S

G

G

G

A

N

A

K

R

Q

F

L

K

E

G

E

I

L

R

R

V

Q

N

N

D

A

A

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

K

K

K

L

L

R

R

G

D

M

L

M

M

P

S

W

W

I

V

G

D

K

R

D192 (= D191) binding Mg(2+); binding Mg(2+)
E196 (= E195) binding Mg(2+)
E228 (= E227) binding Mg(2+)
E232 (= E231) binding Mg(2+)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

4ypoA Crystal structure of mycobacterium tuberculosis ketol-acid reductoisomerase in complex with mg2+ (see paper)
56% identity, 96% coverage: 3:328/339 of query aligns to 1:325/325 of 4ypoA

query
sites
4ypoA

V

M

Y

F

Y

Y

D

D

R

D

D

D

A

A

D

D

L

L

N

S

L

I

I

I

K

Q

S

G

K

R

K

K

V

V

A

G

I

V

I

I

G

G

Y

Y

G

G

S

S

Q

Q

G

G

R

H

A

A

H

H

A

S

L

L

N

S

L

L

K

R

D

D

S

S

G

G

A

V

Q

Q

N

-

V

V

A

R

I

V

A

G

L

L

K

K

S

Q

G

G

S

S

A

R

T

S

A

R

K

P

K

K

A

V

E

E

A

E

D

Q

G

G

F

L

K

D

V

V

M

D

T

T

V

P

A

A

E

E

A

V

A

A

A

K

W

W

A

A

D

D

L

V

M

V

M

M

M

V

A

L

T

A

P

P

D

D

E

T

L

A

Q

Q

A

A

D

E

I

I

Y

F

K

A

A

G

E

D

I

I

A

E

G

P

N

N

I

L

R

K

D

P

G

G

A

D

A

A

I

L

A

F

F

F

A

G

H

H

G

G

L

L

N

N

V

V

H

H

F

F

G

G

L

L

I

I

E

K

P

P

K

P

A

A

S

D

V

V

D

A

V

V

V

A

M

M

I

V

A

A

P

P

K
|
K

G

G

P

P

G

G

H

H

T

L

V

V

R

R

G

R

E

Q

Y

F

Q

V

K

D

G

G

G

K

G

G

V

V

P

P

C

C

L

L

V

V

A

A

V

V

H

E

Q

Q

D

D

A

P

S

R

G

G

N

D

A

G

L

L

D

A

L

L

A

A

L

L

S

S

Y

Y

A

A

C

K

G

A

V

I

G

G

G

T

R

R

S

A

G

G

I

V

I

I

E

K

T

T

N

T

F

F

K

K

E

D

E

E

C

T

E

E

T

T

D
|
D

L

L

F

F

G

G

E
|
E

Q

Q

V

T

V

V

L

L

C

C

G

G

L

T

V

E

E

E

L

L

I

V

R

K

A

A

G

G

F

F

E

E

T

V

L

M

V

V

E

E

A

A

G

G

Y

Y

A

P

P

A

E

E

M

L

A

A

Y

Y

F

F

E
|
E

C

V

L

L

H

H

E
|
E

V

L

K

K

L

L

I

I

V

V

D

D

L

L

I

M

Y

Y

E

E

G

G

I

L

A

A

N

R

M

M

N

Y

Y

Y

S

S

I

V

S

S

N

D

T

T

A

A

E

E

W

F

G

G

E

G

Y

Y

V

L

T

S

G

G

P

P

R

R

I

V

I

I

T

D

E

A

E

G

T

T

K

K

A

E

E

R

M

M

K

R

R

D

V

I

L

L

K

R

D

E

I

I

Q

Q

T

D

G

G

K

S

F

F

T

V

S

H

E

K

W

L

M

V

Q

A

E

D

Y

V

R

E

S

G

G

G

A

N

A

K

R

Q

F

L

K

E

G

E

I

L

R

R

V

Q

N

N

D

A

A

E

H

H

Q

P

I

I

E

E

E

V

V

V

G

G

A

K

K

K

L

L

R

R

G

D

M

L

M

M

P

S

W

W

I

V

active site: K128 (= K131), D188 (= D191), E192 (= E195)
binding magnesium ion: D188 (= D191), E192 (= E195), E224 (= E227), E228 (= E231)

6vo2A Crystal structure of staphylococcus aureus ketol-acid reductoisomerase in complex with mg, NADPH and inhibitor. (see paper)
57% identity, 96% coverage: 3:328/339 of query aligns to 3:326/326 of 6vo2A

query
sites
6vo2A

V

V

Y

Y

D

D

R

Q

D

D

A

V

D

K

L

T

N

D

L

A

I

L

K

Q

S

G

K

K

V

I

A

A

I

V

G

G

Y
|
Y

G
|
G

S
|
S

Q
|
Q

G

G

R

H

A

A

H

H

A

A

L

Q

N

N

L

L

K

K

D

D

S

N

G

G

A

Y

Q

-

N

D

V

V

A

V

I

I

A

G

L
x
I

K
x
R

S

P

G

G

S

R

A

-

T
x
S

A

F

K

D

K

K

A

A

E

K

A

E

D

D

G

G

F

F

K

D

V

V

M

F

T

P

V

V

A

A

E

E

A

A

A

V

A

K

W

Q

A

A

D

D

L

V

M

I

M

M

M

V

A
x
L

T
x
L

P
|
P

D
|
D

E

E

L

I

Q

Q

A

G

D

D

I

V

Y

Y

K

K

A

N

E

E

I

I

A

E

G

P

N

N

I

L

R

E

D

K

G

H

A

N

A

A

I

L

A

A

F

F

A

A

H
|
H

G

G

L

F

N

N

V

I

H

H

F

F

G

G

L

V

I

I

E

Q

P

P

K

P

A

A

S

D

V

V

D

D

V

V

V

F

M

L

I

V

A

A

P

P

K

K

G
|
G

P
|
P

G

G

H

H

T

L

V

V

R

R

G

R

E

T

Y

F

Q

V

K

E

G

G

G

S

G

A

V

V

P

P

C

S

L

L