SitesBLAST
Comparing WP_011976226.1 NCBI__GCF_000017145.1:WP_011976226.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6ymfA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the plp-serine external aldimine state (see paper)
51% identity, 97% coverage: 15:425/425 of query aligns to 9:416/418 of 6ymfA
- active site: Y54 (≠ T60), E56 (= E62), D200 (= D206), T226 (= T232), K229 (= K235), R235 (= R241)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S34 (= S40), S96 (= S102), G97 (= G103), A98 (≠ T104), H125 (= H131), S175 (= S181), D200 (= D206), A202 (= A208), H203 (= H209), T226 (= T232), K229 (= K235), R361 (= R367)
6ymdA Crystal structure of serine hydroxymethyltransferase from aphanothece halophytica in the covalent complex with malonate (see paper)
51% identity, 97% coverage: 15:425/425 of query aligns to 9:416/420 of 6ymdA
- active site: Y54 (≠ T60), E56 (= E62), D200 (= D206), T226 (= T232), K229 (= K235), R235 (= R241)
- binding malonate ion: S34 (= S40), Y54 (≠ T60), E56 (= E62), Y64 (≠ H70), H125 (= H131), H203 (= H209), K229 (= K235), R361 (= R367)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: Y54 (≠ T60), S96 (= S102), G97 (= G103), A98 (≠ T104), H125 (= H131), Y174 (≠ G180), S175 (= S181), D200 (= D206), A202 (= A208), T226 (= T232), K229 (= K235), G261 (≠ S267)
4n0wA X-ray crystal structure of a serine hydroxymethyltransferase from burkholderia cenocepacia with covalently attached pyridoxal phosphate
53% identity, 96% coverage: 17:423/425 of query aligns to 14:415/416 of 4n0wA
- active site: Y57 (≠ T60), E59 (= E62), D202 (= D206), T228 (= T232), K231 (= K235), R237 (= R241)
- binding pyridoxal-5'-phosphate: S99 (= S102), G100 (= G103), S101 (≠ T104), H128 (= H131), D202 (= D206), A204 (= A208), H205 (= H209), K231 (= K235)
4otlA X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and glycine
53% identity, 96% coverage: 17:423/425 of query aligns to 7:408/409 of 4otlA
- active site: Y50 (≠ T60), E52 (= E62), D195 (= D206), T221 (= T232), K224 (= K235), R230 (= R241)
- binding glycine: S30 (= S40), Y50 (≠ T60), Y60 (≠ H70), H121 (= H131), K224 (= K235), R355 (= R367)
- binding pyridoxal-5'-phosphate: S92 (= S102), G93 (= G103), S94 (≠ T104), H121 (= H131), S170 (= S181), D195 (= D206), A197 (= A208), H198 (= H209), K224 (= K235)
4ot8A X-ray crystal structure of serine hydroxymethyl transferase from burkholderia cenocepacia bound to plp and serine
53% identity, 96% coverage: 17:423/425 of query aligns to 12:413/414 of 4ot8A
- active site: Y55 (≠ T60), E57 (= E62), D200 (= D206), T226 (= T232), K229 (= K235), R235 (= R241)
- binding pyridoxal-5'-phosphate: S97 (= S102), G98 (= G103), S99 (≠ T104), H126 (= H131), D200 (= D206), A202 (= A208), H203 (= H209), K229 (= K235)
- binding serine: S35 (= S40), E57 (= E62), Y65 (≠ H70), H126 (= H131), H203 (= H209), R360 (= R367)
2vmyA Crystal structure of f351gbsshmt in complex with gly and fthf (see paper)
52% identity, 95% coverage: 15:417/425 of query aligns to 6:404/405 of 2vmyA
- active site: Y51 (≠ T60), E53 (= E62), D197 (= D206), T223 (= T232), K226 (= K235), R232 (= R241)
- binding N-[4-({[(6S)-2-amino-5-formyl-4-oxo-3,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)benzoyl]-L-glutamic acid: E53 (= E62), Y60 (≠ F69), Y61 (≠ H70), L117 (= L126), G121 (= G130), H122 (= H131), L123 (= L132), S172 (= S181), K248 (≠ S258), F251 (= F261), N341 (= N351), S349 (≠ R359), P350 (= P360), G351 (≠ P361), R357 (= R367)
- binding glycine: S31 (= S40), Y51 (≠ T60), Y61 (≠ H70), H200 (= H209), K226 (= K235), R357 (= R367)
- binding pyridoxal-5'-phosphate: Y51 (≠ T60), S93 (= S102), G94 (= G103), A95 (≠ T104), H122 (= H131), S172 (= S181), D197 (= D206), A199 (= A208), H200 (= H209), T223 (= T232), K226 (= K235), G257 (≠ S267)
2vmxA Crystal structure of f351gbsshmt in complex with l-allo-thr (see paper)
52% identity, 95% coverage: 15:417/425 of query aligns to 6:404/405 of 2vmxA
- active site: Y51 (≠ T60), E53 (= E62), D197 (= D206), T223 (= T232), K226 (= K235), R232 (= R241)
- binding allo-threonine: S31 (= S40), H122 (= H131), H200 (= H209), R357 (= R367)
- binding pyridoxal-5'-phosphate: S93 (= S102), G94 (= G103), A95 (≠ T104), H122 (= H131), S172 (= S181), D197 (= D206), A199 (= A208), H200 (= H209), T223 (= T232), K226 (= K235)
1kl2A Crystal structure of serine hydroxymethyltransferase complexed with glycine and 5-formyl tetrahydrofolate (see paper)
52% identity, 95% coverage: 15:417/425 of query aligns to 6:404/405 of 1kl2A
- active site: Y51 (≠ T60), E53 (= E62), D197 (= D206), T223 (= T232), K226 (= K235), R232 (= R241)
- binding N-{[4-({[(6R)-2-amino-5-formyl-4-oxo-1,4,5,6,7,8-hexahydropteridin-6-yl]methyl}amino)phenyl]carbonyl}-L-glutamic acid: E53 (= E62), Y60 (≠ F69), G121 (= G130), H122 (= H131), S172 (= S181), F251 (= F261), N341 (= N351)
- binding glycine: S31 (= S40), Y51 (≠ T60), Y61 (≠ H70), H200 (= H209), R357 (= R367)
- binding pyridoxal-5'-phosphate: S93 (= S102), G94 (= G103), A95 (≠ T104), H122 (= H131), S172 (= S181), D197 (= D206), A199 (= A208), H200 (= H209), T223 (= T232), H225 (≠ T234), K226 (= K235)
1kl1A Crystal structure of serine hydroxymethyltransferase complexed with glycine (see paper)
52% identity, 95% coverage: 15:417/425 of query aligns to 6:404/405 of 1kl1A
- active site: Y51 (≠ T60), E53 (= E62), D197 (= D206), T223 (= T232), K226 (= K235), R232 (= R241)
- binding glycine: S31 (= S40), H122 (= H131), R357 (= R367)
- binding pyridoxal-5'-phosphate: S93 (= S102), G94 (= G103), A95 (≠ T104), H122 (= H131), A171 (≠ G180), S172 (= S181), D197 (= D206), A199 (= A208), H200 (= H209), T223 (= T232), H225 (≠ T234), K226 (= K235)
1kkpA Crystal structure of serine hydroxymethyltransferase complexed with serine (see paper)
52% identity, 95% coverage: 15:417/425 of query aligns to 6:404/405 of 1kkpA
- active site: Y51 (≠ T60), E53 (= E62), D197 (= D206), T223 (= T232), K226 (= K235), R232 (= R241)
- binding pyridoxal-5'-phosphate: S93 (= S102), G94 (= G103), A95 (≠ T104), H122 (= H131), S172 (= S181), D197 (= D206), A199 (= A208), H200 (= H209), K226 (= K235)
- binding serine: S31 (= S40), H122 (= H131), R357 (= R367)
1kkjA Crystal structure of serine hydroxymethyltransferase from b.Stearothermophilus (see paper)
52% identity, 95% coverage: 15:417/425 of query aligns to 6:404/405 of 1kkjA
- active site: Y51 (≠ T60), E53 (= E62), D197 (= D206), T223 (= T232), K226 (= K235), R232 (= R241)
- binding pyridoxal-5'-phosphate: S93 (= S102), G94 (= G103), A95 (≠ T104), H122 (= H131), S172 (= S181), D197 (= D206), A199 (= A208), H200 (= H209), T223 (= T232), H225 (≠ T234), K226 (= K235)
7x5oB Crystal structure of e. Faecium shmt in complex with me-thf and plp- gly (see paper)
50% identity, 96% coverage: 17:423/425 of query aligns to 7:411/412 of 7x5oB
- binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: S30 (= S40), Y50 (≠ T60), Y60 (≠ H70), S92 (= S102), G93 (= G103), S94 (≠ T104), H121 (= H131), S171 (= S181), D196 (= D206), A198 (= A208), H199 (= H209), K225 (= K235), R358 (= R367)
- binding n-[4-({[(6s)-2-amino-4-hydroxy-5-methyl-5,6,7,8-tetrahydropteridin-6-yl]methyl}amino)benzoyl]-l-glutamic acid: E52 (= E62), Y59 (≠ F69), L116 (= L126), G119 (= G129), G120 (= G130), H121 (= H131), S171 (= S181), P252 (= P262), N342 (= N351), P351 (= P360)
7x5nA Crystal structure of e. Faecium shmt in complex with (+)-shin-1 and plp-ser (see paper)
50% identity, 96% coverage: 17:422/425 of query aligns to 6:409/409 of 7x5nA
- binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E62), Y58 (≠ F69), Y59 (≠ H70), L115 (= L126), G119 (= G130), H120 (= H131), L121 (= L132), K340 (= K350), N341 (= N351), S342 (≠ P352), P350 (= P360), F351 (≠ P361), R357 (= R367)
- binding [3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-serine: S29 (= S40), Y49 (≠ T60), E51 (= E62), Y59 (≠ H70), S91 (= S102), G92 (= G103), S93 (≠ T104), H120 (= H131), S170 (= S181), D195 (= D206), A197 (= A208), H198 (= H209), K224 (= K235), R357 (= R367)
7v3dA Complex structure of serine hydroxymethyltransferase from enterococcus faecium and its inhibitor (see paper)
50% identity, 96% coverage: 17:422/425 of query aligns to 6:409/409 of 7v3dA
- binding (4R)-6-azanyl-4-[3-(hydroxymethyl)-5-phenyl-phenyl]-3-methyl-4-propan-2-yl-1H-pyrano[2,3-c]pyrazole-5-carbonitrile: E51 (= E62), Y58 (≠ F69), L115 (= L126), G119 (= G130), H120 (= H131), L121 (= L132), K340 (= K350), S342 (≠ P352), P350 (= P360), F351 (≠ P361), R357 (= R367)
- binding pyridoxal-5'-phosphate: Y49 (≠ T60), S91 (= S102), G92 (= G103), S93 (≠ T104), H120 (= H131), S170 (= S181), D195 (= D206), A197 (= A208), K224 (= K235), G255 (= G266)
Q5SI56 Serine hydroxymethyltransferase; SHMT; Serine methylase; EC 2.1.2.1 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
50% identity, 96% coverage: 15:422/425 of query aligns to 6:406/407 of Q5SI56
- Y51 (≠ T60) binding pyridoxal 5'-phosphate
- GS 94:95 (≠ GT 103:104) binding pyridoxal 5'-phosphate
- S172 (= S181) binding pyridoxal 5'-phosphate
- H200 (= H209) binding pyridoxal 5'-phosphate
- H225 (≠ T234) binding pyridoxal 5'-phosphate
- K226 (= K235) modified: N6-(pyridoxal phosphate)lysine
- G258 (≠ S267) binding pyridoxal 5'-phosphate
8suiB Joint x-ray/neutron structure of thermus thermophilus serine hydroxymethyltransferase (tthshmt) in internal aldimine state with l- ser bound in a pre-michalis complex (see paper)
50% identity, 96% coverage: 15:422/425 of query aligns to 1:401/402 of 8suiB
8ssyA Room-temperature x-ray structure of thermus thermophilus serine hydroxymethyltransferase (shmt) bound with d-ser in a pseudo- michaelis complex (see paper)
50% identity, 96% coverage: 15:422/425 of query aligns to 1:401/402 of 8ssyA
2dkjA Crystal structure of t.Th.Hb8 serine hydroxymethyltransferase
50% identity, 96% coverage: 15:422/425 of query aligns to 1:401/402 of 2dkjA
- active site: Y46 (≠ T60), E48 (= E62), D192 (= D206), T218 (= T232), K221 (= K235), R227 (= R241)
- binding pyridoxal-5'-phosphate: S88 (= S102), G89 (= G103), S90 (≠ T104), H117 (= H131), S167 (= S181), D192 (= D206), A194 (= A208), H220 (≠ T234), K221 (= K235)
3pgyB Serine hydroxymethyltransferase from staphylococcus aureus, s95p mutant.
50% identity, 97% coverage: 13:423/425 of query aligns to 4:404/404 of 3pgyB
6tghC Shmt from streptococcus thermophilus tyr55thr variant in complex with d-serine both as external aldimine and as non-covalent complex
51% identity, 96% coverage: 17:423/425 of query aligns to 6:410/410 of 6tghC
- active site: T49 (= T60), E51 (= E62), D195 (= D206), T221 (= T232), K224 (= K235), R230 (= R241)
- binding d-serine: S29 (= S40), H198 (= H209), R357 (= R367)
- binding L-Serine, N-[[3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]-4-pyridinyl]methylene]: E51 (= E62), G255 (= G266)
- binding pyridoxal-5'-phosphate: S91 (= S102), G92 (= G103), S93 (≠ T104), H120 (= H131), D195 (= D206), A197 (= A208), H198 (= H209), T221 (= T232), K224 (= K235)
Query Sequence
>WP_011976226.1 NCBI__GCF_000017145.1:WP_011976226.1
MTEYTKAYFNAPVHERDPLVAQAIDNERKRQQDQIELIASENIVSRAVLDALGHEMTNKT
LEGYPGNRFHGGGQFVDVVEQAAIDRAKQLFGCAYANVQPHSGTQANLAVFFLLLTPGDK
VLSLDLAAGGHLSHGMKGNLSGRWFEPHNYNVNPETEVIDYDELERIAEEVRPTLLITGG
SAYPRELDFERMGNIAKKVGAWFLVDMAHIAGLVAGGVHPSPFPHADIVTCTTTKTLRGP
RGGLILTNNEAWFKKLQSAVFPGVQGSLHSNVLAAKAICLGEALRDDFKVYAAQVKTNAR
VLADVLMARGVRVVSGGTDTHIVLVDLSSKGLIGKQAEDLLARANITANKNPIPNDSPRP
PEWLGMRLGVSAATTRGMKEDEFRTLGTIIADLIEAEAAGNADLSVEAAKTKVAELTAAF
PVYGH
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory