SitesBLAST
Comparing WP_011988737.1 NCBI__GCF_000016505.1:WP_011988737.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
7p8na Tmhydabc- t. Maritima hydrogenase with bridge closed (see paper)
31% identity, 62% coverage: 111:387/447 of query aligns to 180:489/642 of 7p8na
- binding iron/sulfur cluster: C186 (= C118), V187 (≠ T119), L188 (≠ D120), C189 (= C121), G190 (= G122), C192 (= C124), C196 (= C128), P197 (= P129), T198 (≠ N130), A200 (≠ S132), L201 (≠ I133), C295 (= C220), C350 (= C267), M480 (= M378), A481 (≠ G379), C482 (= C380), C486 (= C384)
Sites not aligning to the query:
- binding fe2/s2 (inorganic) cluster: 32, 34, 42, 43, 45, 46, 48, 60, 575, 577, 579, 580, 612, 613, 615, 616
- binding iron/sulfur cluster: 92, 95, 96, 99, 105, 108, 143, 144, 145, 146, 147, 148, 149, 153, 161
5oefA Active semisynthetic [fefe]-hydrogenase cpi with aza-diselenato- bridged [2fe] cofactor (see paper)
25% identity, 85% coverage: 18:395/447 of query aligns to 80:516/574 of 5oefA
- active site: E279 (= E199), E282 (= E202), C299 (= C219), S319 (= S239), K358 (= K270), E361 (= E273), Q366 (vs. gap), C503 (= C384)
- binding dicarbonyl[bis(cyanide-kappaC)]-mu-(iminodimethaneselenato- 1kappaS:2kappaS)-mu-(oxomethylidene)diiron(2+): A230 (= A157), P231 (= P158), S232 (≠ A159), C299 (= C219), P324 (= P244), Q325 (≠ M245), M353 (≠ G265), P354 (= P266), K358 (= K270), F417 (≠ Y322), M497 (= M378), C503 (= C384)
- binding iron/sulfur cluster: H94 (= H32), F96 (≠ L34), K97 (vs. gap), C98 (vs. gap), C101 (vs. gap), R103 (= R36), R104 (≠ E37), C107 (= C40), L110 (≠ I43), C147 (≠ I75), L148 (≠ N76), L149 (≠ K77), C150 (≠ V78), G151 (≠ T79), C153 (= C81), C157 (= C85), T161 (≠ D89), A165 (≠ C93), M166 (≠ E94), I177 (≠ H105), C190 (= C118), L191 (≠ T119), L192 (≠ D120), C193 (= C121), G194 (= G122), C196 (= C124), C200 (= C128), P201 (= P129), V202 (≠ N130), A204 (≠ S132), L205 (≠ I133), C300 (= C220), C355 (= C267), M497 (= M378), A498 (≠ G379), C499 (= C380), C503 (= C384)
Sites not aligning to the query:
3c8yA 1.39 angstrom crystal structure of fe-only hydrogenase (see paper)
25% identity, 85% coverage: 18:395/447 of query aligns to 80:516/574 of 3c8yA
- active site: E279 (= E199), E282 (= E202), C299 (= C219), S319 (= S239), K358 (= K270), E361 (= E273), Q366 (vs. gap), C503 (= C384)
- binding 2 iron/2 sulfur/3 carbonyl/2 cyanide/water/methylether cluster: A230 (= A157), P231 (= P158), S232 (≠ A159), C299 (= C219), P324 (= P244), Q325 (≠ M245), M353 (≠ G265), P354 (= P266), K358 (= K270), F417 (≠ Y322), M497 (= M378), C503 (= C384)
- binding iron/sulfur cluster: H94 (= H32), F96 (≠ L34), K97 (vs. gap), C98 (vs. gap), C101 (vs. gap), R104 (≠ E37), C107 (= C40), L110 (≠ I43), C147 (≠ I75), L148 (≠ N76), L149 (≠ K77), C150 (≠ V78), G151 (≠ T79), C153 (= C81), C157 (= C85), T161 (≠ D89), A165 (≠ C93), M166 (≠ E94), I177 (≠ H105), C190 (= C118), L191 (≠ T119), L192 (≠ D120), C193 (= C121), G194 (= G122), Q195 (≠ F123), C196 (= C124), C200 (= C128), P201 (= P129), A203 (≠ G131), A204 (≠ S132), L205 (≠ I133), C300 (= C220), C355 (= C267), M497 (= M378), A498 (≠ G379), C499 (= C380), C503 (= C384)
Sites not aligning to the query:
1c4cA Binding of exogenously added carbon monoxide at the active site of the fe-only hydrogenase (cpi) from clostridium pasteurianum (see paper)
25% identity, 85% coverage: 18:395/447 of query aligns to 80:516/574 of 1c4cA
- active site: E279 (= E199), E282 (= E202), C299 (= C219), S319 (= S239), K358 (= K270), E361 (= E273), Q366 (vs. gap), C503 (= C384)
- binding 2 iron/2 sulfur/6 carbonyl/1 water inorganic cluster: A230 (= A157), P231 (= P158), S232 (≠ A159), C299 (= C219), P324 (= P244), Q325 (≠ M245), M353 (≠ G265), P354 (= P266), K358 (= K270), F417 (≠ Y322), M497 (= M378), C503 (= C384)
- binding iron/sulfur cluster: H94 (= H32), F96 (≠ L34), K97 (vs. gap), C98 (vs. gap), C101 (vs. gap), R104 (≠ E37), C107 (= C40), C147 (≠ I75), L148 (≠ N76), L149 (≠ K77), C150 (≠ V78), C153 (= C81), C157 (= C85), M166 (≠ E94), I177 (≠ H105), C190 (= C118), L191 (≠ T119), L192 (≠ D120), C193 (= C121), G194 (= G122), C196 (= C124), C200 (= C128), P201 (= P129), V202 (≠ N130), A203 (≠ G131), A204 (≠ S132), L205 (≠ I133), C300 (= C220), C355 (= C267), M497 (= M378), A498 (≠ G379), C499 (= C380), C503 (= C384), G506 (vs. gap)
Sites not aligning to the query:
1c4aA Binding of exogenously added carbon monoxide at the active site of the fe-only hydrogenase (cpi) from clostridium pasteurianum (see paper)
25% identity, 85% coverage: 18:395/447 of query aligns to 80:516/574 of 1c4aA
- active site: E279 (= E199), E282 (= E202), C299 (= C219), S319 (= S239), K358 (= K270), E361 (= E273), Q366 (vs. gap), C503 (= C384)
- binding 2 iron/2 sulfur/5 carbonyl/2 water inorganic cluster: A230 (= A157), P231 (= P158), S232 (≠ A159), C299 (= C219), P324 (= P244), M353 (≠ G265), P354 (= P266), K358 (= K270), F417 (≠ Y322), M497 (= M378), C503 (= C384)
- binding iron/sulfur cluster: H94 (= H32), K97 (vs. gap), C98 (vs. gap), C101 (vs. gap), R104 (≠ E37), C107 (= C40), L140 (≠ Y68), K146 (≠ I74), C147 (≠ I75), L148 (≠ N76), L149 (≠ K77), C150 (≠ V78), G151 (≠ T79), C153 (= C81), C157 (= C85), T161 (≠ D89), A165 (≠ C93), M166 (≠ E94), I177 (≠ H105), C190 (= C118), L191 (≠ T119), L192 (≠ D120), C193 (= C121), G194 (= G122), C196 (= C124), C200 (= C128), P201 (= P129), V202 (≠ N130), A203 (≠ G131), A204 (≠ S132), L205 (≠ I133), C300 (= C220), C355 (= C267), M497 (= M378), A498 (≠ G379), C499 (= C380), C503 (= C384), G506 (vs. gap)
Sites not aligning to the query:
P29166 Iron hydrogenase 1; CpI; Fe-only hydrogenase; [Fe] hydrogenase; EC 1.12.7.2 from Clostridium pasteurianum (see 2 papers)
25% identity, 85% coverage: 18:395/447 of query aligns to 80:516/574 of P29166
- H94 (= H32) binding [4Fe-4S] cluster
- C98 (vs. gap) binding [4Fe-4S] cluster
- C101 (vs. gap) binding [4Fe-4S] cluster
- C107 (= C40) binding [4Fe-4S] cluster
- C147 (≠ I75) binding [4Fe-4S] cluster
- C150 (≠ V78) binding [4Fe-4S] cluster
- C153 (= C81) binding [4Fe-4S] cluster
- C157 (= C85) binding [4Fe-4S] cluster
- C190 (= C118) binding [4Fe-4S] cluster
- C193 (= C121) binding [4Fe-4S] cluster
- C196 (= C124) binding [4Fe-4S] cluster
- C200 (= C128) binding [4Fe-4S] cluster
- C300 (= C220) binding [4Fe-4S] cluster
- C355 (= C267) binding [4Fe-4S] cluster
- C499 (= C380) binding [4Fe-4S] cluster
- C503 (= C384) binding [4Fe-4S] cluster; binding Fe(2+)
Sites not aligning to the query:
- 34 binding [2Fe-2S] cluster
- 46 binding [2Fe-2S] cluster
- 49 binding [2Fe-2S] cluster
- 62 binding [2Fe-2S] cluster
5bysA Semisynthetic [fefe]-hydrogenase cpi with sulfur-dithiolato-bridged [2fe] cofactor (see paper)
25% identity, 85% coverage: 18:395/447 of query aligns to 79:515/576 of 5bysA
- active site: E278 (= E199), E281 (= E202), C298 (= C219), S318 (= S239), K357 (= K270), E360 (= E273), Q365 (vs. gap), C502 (= C384)
- binding bis(cyanido-kappaC)(dicarbonyl)-mu-(oxomethylidene)-mu-(sulfanediyldimethanethiolatato-1kappaS:2kappaS)diiron(2+): A229 (= A157), P230 (= P158), S231 (≠ A159), I267 (≠ V188), C298 (= C219), P323 (= P244), Q324 (≠ M245), M352 (≠ G265), P353 (= P266), K357 (= K270), F416 (≠ Y322), V422 (= V328), M496 (= M378), C502 (= C384)
- binding iron/sulfur cluster: H93 (= H32), F95 (≠ L34), K96 (vs. gap), C97 (vs. gap), C100 (vs. gap), C106 (= C40), K145 (≠ I74), C146 (≠ I75), L147 (≠ N76), L148 (≠ K77), C149 (≠ V78), G150 (≠ T79), C152 (= C81), C156 (= C85), T160 (≠ D89), A164 (≠ C93), M165 (≠ E94), I176 (≠ H105), C189 (= C118), L190 (≠ T119), L191 (≠ D120), C192 (= C121), G193 (= G122), C195 (= C124), C199 (= C128), P200 (= P129), V201 (≠ N130), A202 (≠ G131), A203 (≠ S132), L204 (≠ I133), C299 (= C220), C354 (= C267), M496 (= M378), A497 (≠ G379), C498 (= C380), C502 (= C384)
Sites not aligning to the query:
8aioA Co-bound [fefe]-hydrogenase i from clostridium pasteurianum (cpi) (see paper)
25% identity, 85% coverage: 18:395/447 of query aligns to 80:516/573 of 8aioA
- binding Binuclear [FeFe], di(thiomethyl)amine, carbon monoxide, cyanide cluster (-CO form): A230 (= A157), P231 (= P158), S232 (≠ A159), C299 (= C219), P324 (= P244), Q325 (≠ M245), M353 (≠ G265), P354 (= P266), K358 (= K270), F417 (≠ Y322), M497 (= M378), C503 (= C384)
- binding iron/sulfur cluster: H94 (= H32), F96 (≠ L34), K97 (vs. gap), C98 (vs. gap), C101 (vs. gap), C107 (= C40), K146 (≠ I74), C147 (≠ I75), L148 (≠ N76), L149 (≠ K77), C150 (≠ V78), G151 (≠ T79), C153 (= C81), C157 (= C85), A165 (≠ C93), M166 (≠ E94), I177 (≠ H105), C190 (= C118), L191 (≠ T119), L192 (≠ D120), C193 (= C121), G194 (= G122), C196 (= C124), C200 (= C128), P201 (= P129), V202 (≠ N130), A204 (≠ S132), L205 (≠ I133), C300 (= C220), C355 (= C267), M497 (= M378), C499 (= C380), C503 (= C384)
Sites not aligning to the query:
6h63A Semisynthetic [fefe]-hydrogenase cpi with ethanedithiolate [2fe] cofactor
25% identity, 85% coverage: 18:395/447 of query aligns to 79:515/578 of 6h63A
- active site: E278 (= E199), E281 (= E202), C298 (= C219), S318 (= S239), K357 (= K270), E360 (= E273), Q365 (vs. gap), C502 (= C384)
- binding dicarbonyl[bis(cyanide-kappaC)]-mu-(ethanethiolatato-1kappaS:2kappaS)-mu-(ox omethylidene)diiron(2+): A229 (= A157), P230 (= P158), S231 (≠ A159), C298 (= C219), P323 (= P244), Q324 (≠ M245), M352 (≠ G265), P353 (= P266), K357 (= K270), F416 (≠ Y322), M496 (= M378), C502 (= C384)
- binding iron/sulfur cluster: H93 (= H32), F95 (≠ L34), K96 (vs. gap), C97 (vs. gap), C100 (vs. gap), R102 (= R36), C106 (= C40), C146 (≠ I75), L147 (≠ N76), L148 (≠ K77), C149 (≠ V78), G150 (≠ T79), C152 (= C81), C156 (= C85), T160 (≠ D89), A164 (≠ C93), M165 (≠ E94), I176 (≠ H105), C189 (= C118), L190 (≠ T119), L191 (≠ D120), C192 (= C121), G193 (= G122), C195 (= C124), C199 (= C128), P200 (= P129), V201 (≠ N130), A203 (≠ S132), L204 (≠ I133), C299 (= C220), C354 (= C267), A497 (≠ G379), C498 (= C380), C502 (= C384)
Sites not aligning to the query:
5byrA Semisynthetic [fefe]-hydrogenase cpi with propane-dithiolato-bridged [2fe] cofactor (see paper)
25% identity, 85% coverage: 18:395/447 of query aligns to 79:515/571 of 5byrA