SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011989078.1 NCBI__GCF_000016505.1:WP_011989078.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4d8uH Crystal structure of d-cysteine desulfhydrase from salmonella typhimurium at 3.3 a in monoclinic space group with 8 subunits in the asymmetric unit (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 14:325/331 of 4d8uH

query
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4d8uH

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active site: K54 (= K48), Y264 (= Y260), Y290 (= Y286)
binding phosphate ion: K54 (= K48), S81 (= S75), H83 (= H77), Y290 (= Y286)

4d9fA D-cysteine desulfhydrase from salmonella typhimurium complexed with d- cycloserine (dcs) (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 11:322/328 of 4d9fA

query
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active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding d-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl]-n,o-cycloserylamide: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), G194 (= G190), S195 (= S191), A196 (≠ G192), T198 (= T194), Y287 (= Y286), T315 (= T314), G316 (= G315), G317 (= G316)

4d9eA D-cysteine desulfhydrase from salmonella typhimurium complexed with l- cycloserine (lcs) (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 11:322/328 of 4d9eA

query
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4d9eA

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active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: N50 (= N47), K51 (= K48), N79 (= N76), G194 (= G190), S195 (= S191), A196 (≠ G192), T198 (= T194), Y287 (= Y286), T315 (= T314), G316 (= G315), G317 (= G316)

4d9bA Pyridoxamine 5' phosphate (pmp) bound form of salmonella typhimurium d-cysteine desulfhydrase obtained after co-crystallization with d- cycloserine (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 11:322/328 of 4d9bA

query
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4d9bA

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F

A

I

V

K

K

E

L

L

L

A

A

E

S

L

L

E

E

G

G

I

V

I

L

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

Y

A

G

G

L

L

T

I

Q

D

E

G

I

I

K

S

K

Q

G

K

K

R

F

F

S

N

K

D

Y

D

K

G

N

P

L

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

I

A

F

P

G

A

I

L

F

F

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), N79 (= N76), G194 (= G190), S195 (= S191), A196 (≠ G192), T198 (= T194), Y287 (= Y286), T315 (= T314), G316 (= G315), G317 (= G316)

4d99A Salmonella typhimurium d-cysteine desulfhydrase with l-ser bound non- covalently at the active site (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 11:322/328 of 4d99A

query
sites
4d99A

R

R

M

L

N

E

M

F

A

I

N

G

L

A

P

P

T

T

K

P

I

L

E

E

K

Y

M

L

E

P

R

R

L

L

S

S

Q

D

K

Y

L

L

G

G

G

R

P

-

D

E

I

I

Y

Y

I

I

K

K

R

R

D

D

Q

V

T

T

G

P

T

I

E

A

I

M

S

G

G

G

N

N

K
|
K

I

L

R

R

K

K

L

L

E

E

F

F

S

L

A

V

A

A

E

D

A

A

L

L

N

R

K

E

G

G

C

A

N

D

T

T

L

L

I

I

T

T

C

A

G

G

G
x
A

I

I

Q

Q

S
|
S

N

N

H
|
H

C

V

R

R

A

Q

T

T

A

A

V

V

A

A

V

A

K

K

L

L

G

G

F

L

K

H

C

C

C

V

L

A

V

L

L

L

N

E

G

N

S

P

N

I

D

G

T

T

E

T

V

A

D

E

-

N

-

Y

-

L

-

T

-

N

G

G

N

N

L

R

L

L

D

L

K

D

L

L

L

F

G

N

A

T

E

Q

I

I

Y

E

F

M

V

C

S

D

Q

A

K

L

E

T

Y

D

E

P

N

D

R

A

R

Q

M

L

E

Q

I

T

M

L

K

-

E

-

I

-

K

A

T

T

N

R

M

I

E

E

N

A

K

Q

G

G

L

F

K

R

P

P

Y

Y

I

V

I

I

P

P

E

V

G

G

A

G

S

S

N

S

G

A

I

L

G

G

G

A

F

M

G

G

Y

Y

Y

V

K

E

A

S

V

A

Q

L

E

E

I

I

M

A

L

-

Q

Q

E

Q

R

C

E

E

M

E

K

V

V

V

H

G

F

L

D

S

G

S

I

V

V

V

I

V

A

A

T

S

G

G

S

S

G

A

G

G

T

T

Y

H

S

A

G

G

L

L

L

A

L

V

G

G

S

L

R

E

I

H

L

L

N

M

Y

P

D

D

A

V

K

E

I

L

Y

I

G

G

V

V

N

T

V

V

C

S

Q

R

N

S

E

V

K

A

Y

E

F

Q

K

K

D

P

R

K

I

V

Y

I

E

A

I

L

L

Q

H

Q

D

A

S

I

M

A

K

G

Y

Q

I

L

D

A

V

L

N

T

L

-

N

-

F

-

S

A

K

T

D

A

E

D

I

I

N

H

I

L

I

W

D

D

G

D

Y

Y

V

F

G

A

R

P

G

G

Y
|
Y

A

G

L

V

S

P

R

N

E

D

E

A

E

G

L

M

E

E

F

A

I

V

K

K

E

L

L

L

A

A

E

S

L

L

E

E

G

G

I

V

I

L

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

Y

A

G

G

L

L

T

I

Q

D

E

G

I

I

K

S

K

Q

G

K

K

R

F

F

S

N

K

D

Y

D

K

G

N

P

L

I

L

L

F

F

I

I

H

H

T

T

G

G

I

A

F

P

G

A

I

L

F

F

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding serine: K51 (= K48), A75 (≠ G72), S78 (= S75), H80 (= H77), Y261 (= Y260), Y287 (= Y286)

4d97A Salmonella typhimurium d-cysteine desulfhydrase with d-ser bound at active site (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 11:322/328 of 4d97A

query
sites
4d97A

R

R

M

L

N

E

M

F

A

I

N

G

L

A

P

P

T

T

K

P

I

L

E

E

K

Y

M

L

E

P

R

R

L

L

S

S

Q

D

K

Y

L

L

G

G

G

R

P

-

D

E

I

I

Y

Y

I

I

K

K

R

R

D

D

Q

V

T

T

G

P

T

I

E

A

I

M

S

G

G

G

N

N

K
|
K

I

L

R

R

K

K

L

L

E

E

F

F

S

L

A

V

A

A

E

D

A

A

L

L

N

R

K

E

G

G

C

A

N

D

T

T

L

L

I

I

T

T

C

A

G

G

G

A

I

I

Q

Q

S
|
S

N
|
N

H
|
H

C

V

R

R

A

Q

T

T

A

A

V

V

A

A

V

A

K

K

L

L

G

G

F

L

K

H

C

C

C

V

L

A

V

L

L

L

N

E

G

N

S

P

N

I

D

G

T

T

E

T

V

A

D

E

-

N

-

Y

-

L

-

T

-

N

G

G

N

N

L

R

L

L

D

L

K

D

L

L

L

F

G

N

A

T

E

Q

I

I

Y

E

F

M

V

C

S

D

Q

A

K

L

E

T

Y

D

E

P

N

D

R

A

R

Q

M

L

E

Q

I

T

M

L

K

-

E

-

I

-

K

A

T

T

N

R

M

I

E

E

N

A

K

Q

G

G

L

F

K

R

P

P

Y

Y

I

V

I

I

P

P

E

V

G
|
G

A

G

S

S

N

S

G

A

I

L

G

G

G

A

F

M

G

G

Y

Y

Y

V

K

E

A

S

V

A

Q

L

E

E

I

I

M

A

L

-

Q

Q

E

Q

R

C

E

E

M

E

K

V

V

V

H

G

F

L

D

S

G

S

I

V

V

V

I

V

A

A

T

S

G

G

S

S

G

A

G

G

T

T

Y

H

S

A

G

G

L

L

L

A

L

V

G

G

S

L

R

E

I

H

L

L

N

M

Y

P

D

D

A

V

K

E

I

L

Y

I

G

G

V

V

N

T

V

V

C

S

Q

R

N

S

E

V

K

A

Y

E

F

Q

K

K

D

P

R

K

I

V

Y

I

E

A

I

L

L

Q

H

Q

D

A

S

I

M

A

K

G

Y

Q

I

L

D

A

V

L

N

T

L

-

N

-

F

-

S

A

K

T

D

A

E

D

I

I

N

H

I

L

I

W

D

D

G

D

Y

Y

V

F

G

A

R

P

G

G

Y
|
Y

A

G

L

V

S

P

R

N

E

D

E

A

E

G

L

M

E

E

F

A

I

V

K

K

E

L

L

L

A

A

E

S

L

L

E

E

G

G

I

V

I

L

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

Y

A

G

G

L

L

T

I

Q

D

E

G

I

I

K

S

K

Q

G

K

K

R

F

F

S

N

K

D

Y

D

K

G

N

P

L

I

L

L

F

F

I

I

H

H

T

T

G

G

I

A

F

P

G

A

I

L

F

F

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding d-serine: K51 (= K48), S78 (= S75), N79 (= N76), H80 (= H77), G159 (= G154), Y287 (= Y286)

4d96A D-cysteine desulfhydrase from salmonella typhimurium complexed with 1- amino-1-carboxycyclopropane (acc) (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 11:322/328 of 4d96A

query
sites
4d96A

R

R

M

L

N

E

M

F

A

I

N

G

L

A

P

P

T

T

K

P

I

L

E

E

K

Y

M

L

E

P

R

R

L

L

S

S

Q

D

K

Y

L

L

G

G

G

R

P

-

D

E

I

I

Y

Y

I

I

K

K

R

R

D

D

Q

V

T

T

G

P

T

I

E

A

I

M

S

G

G

G

N
|
N

K
|
K

I

L

R

R

K

K

L

L

E

E

F

F

S

L

A

V

A

A

E

D

A

A

L

L

N

R

K

E

G

G

C

A

N

D

T

T

L

L

I

I

T

T

C

A

G

G

G

A

I

I

Q

Q

S
|
S

N
|
N

H
|
H

C

V

R

R

A

Q

T

T

A

A

V

V

A

A

V

A

K

K

L

L

G

G

F

L

K

H

C

C

C

V

L

A

V

L

L

L

N

E

G

N

S

P

N

I

D

G

T

T

E

T

V

A

D

E

-

N

-

Y

-

L

-

T

-

N

G

G

N

N

L

R

L

L

D

L

K

D

L

L

L

F

G

N

A

T

E

Q

I

I

Y

E

F

M

V

C

S

D

Q

A

K

L

E

T

Y

D

E

P

N

D

R

A

R

Q

M

L

E

Q

I

T

M

L

K

-

E

-

I

-

K

A

T

T

N

R

M

I

E

E

N

A

K

Q

G

G

L

F

K

R

P

P

Y

Y

I

V

I

I

P

P

E

V

G

G

A

G

S

S

N

S

G

A

I

L

G

G

G

A

F

M

G

G

Y

Y

Y

V

K

E

A

S

V

A

Q

L

E

E

I

I

M

A

L

-

Q

Q

E

Q

R

C

E

E

M

E

K

V

V

V

H

G

F

L

D

S

G

S

I

V

V

V

I

V

A

A

T

S

G
|
G

S
|
S

G
x
A

G

G

T
|
T

Y

H

S

A

G

G

L

L

L

A

L

V

G

G

S

L

R

E

I

H

L

L

N

M

Y

P

D

D

A

V

K

E

I

L

Y

I

G

G

V

V

N

T

V

V

C

S

Q

R

N

S

E

V

K

A

Y

E

F

Q

K

K

D

P

R

K

I

V

Y

I

E

A

I

L

L

Q

H

Q

D

A

S

I

M

A

K

G

Y

Q

I

L

D

A

V

L

N

T

L

-

N

-

F

-

S

A

K

T

D

A

E

D

I

I

N

H

I

L

I

W

D

D

G

D

Y

Y

V

F

G

A

R

P

G

G

Y
|
Y

A

G

L

V

S

P

R

N

E

D

E

A

E

G

L

M

E

E

F

A

I

V

K

K

E

L

L

L

A

A

E

S

L

L

E

E

G

G

I

V

I

L

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

Y

A

G

G

L

L

T

I

Q

D

E

G

I

I

K

S

K

Q

G

K

K

R

F

F

S

N

K

D

Y

D

K

G

N

P

L

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

I

A

F

P

G

A

I

L

F

F

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: N50 (= N47), K51 (= K48), S78 (= S75), N79 (= N76), H80 (= H77), G194 (= G190), S195 (= S191), A196 (≠ G192), T198 (= T194), Y287 (= Y286), T315 (= T314), G316 (= G315), G317 (= G316)

4d8wA Salmonella typhimurium d-cysteine desulfhydrase soaked with d-cys shows pyruvate bound 4 a away from active site (see paper)
38% identity, 96% coverage: 7:321/329 of query aligns to 11:322/328 of 4d8wA

query
sites
4d8wA

R

R

M

L

N

E

M

F

A

I

N

G

L

A

P

P

T

T

K

P

I

L

E

E

K

Y

M

L

E

P

R

R

L

L

S

S

Q

D

K

Y

L

L

G

G

G

R

P

-

D

E

I

I

Y

Y

I

I

K

K

R

R

D

D

Q

V

T

T

G

P

T

I

E

A

I

M

S

G

G

G

N

N

K
|
K

I

L

R

R

K

K

L

L

E

E

F

F

S

L

A

V

A

A

E

D

A

A

L

L

N

R

K

E

G

G

C

A

N

D

T

T

L

L

I

I

T

T

C

A

G

G

G

A

I

I

Q

Q

S

S

N

N

H

H

C

V

R

R

A

Q

T

T

A

A

V

V

A

A

V

A

K

K

L

L

G

G

F

L

K

H

C

C

C

V

L

A

V

L

L

L

N

E

G

N

S

P

N

I

D

G

T

T

E

T

V

A

D

E

-

N

-

Y

-

L

-

T

-

N

G

G

N

N

L

R

L

L

D

L

K

D

L

L

L

F

G

N

A

T

E

Q

I

I

Y

E

F

M

V

C

S

D

Q

A

K

L

E

T

Y

D

E

P

N

D

R

A

R

Q

M

L

E

Q

I

T

M

L

K

-

E

-

I

-

K

A

T

T

N

R

M

I

E

E

N

A

K

Q

G

G

L

F

K

R

P

P

Y

Y

I

V

I

I

P

P

E

V

G

G

A

G

S

S

N

S

G

A

I

L

G

G

G

A

F

M

G

G

Y

Y

Y

V

K

E

A

S

V

A

Q

L

E

E

I

I

M

A

L

-

Q

Q

E

Q

R

C

E

E

M

E

K

V

V

V

H

G

F

L

D

S

G

S

I

V

V

V

I

V

A

A

T

S

G

G

S
|
S

G

A

G

G

T

T

Y

H

S

A

G

G

L

L

L

A

L

V

G

G

S

L

R

E

I

H

L

L

N

M

Y

P

D

D

A

V

K

E

I

L

Y

I

G

G

V

V

N

T

V

V

C
x
S

Q
x
R

N

S

E

V

K

A

Y

E

F

Q

K

K

D

P

R

K

I

V

Y

I

E

A

I

L

L

Q

H

Q

D

A

S

I

M

A

K

G

Y

Q

I

L

D

A

V

L

N

T

L

-

N

-

F

-

S

A

K

T

D

A

E

D

I

I

N

H

I

L

I

W

D

D

G

D

Y

Y

V

F

G

A

R

P

G

G

Y
|
Y

A

G

L

V

S

P

R

N

E

D

E

A

E

G

L

M

E

E

F

A

I

V

K

K

E

L

L

L

A

A

E

S

L

L

E

E

G

G

I

V

I

L

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

M

Y

A

G

G

L

L

T

I

Q

D

E

G

I

I

K

S

K

Q

G

K

K

R

F

F

S

N

K

D

Y

D

K

G

N

P

L

I

L

L

F

F

I

I

H

H

T

T

G

G

I

A

F

P

G

A

I

L

F

F

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding pyruvic acid: S195 (= S191), S221 (≠ C217), R222 (≠ Q218)

1j0bA Crystal structure analysis of the acc deaminase homologue complexed with inhibitor (see paper)
40% identity, 98% coverage: 1:321/329 of query aligns to 9:315/325 of 1j0bA

query
sites
1j0bA

M

L

I

A

K

K

I

F

P

P

E

-

R

R

M

V

N

E

M

L

A

I

N

P

L

W

P

E

T

T

K

P

I

I

E

Q

K

Y

M

L

E

P

R

N

L

I

S

S

Q

R

K

E

L

I

G

G

G

A

P

-

D

D

I

V

Y

Y

I

I

K

K

R

R

D

D

Q

L

T

T

G

G

T

L

E

G

I

I

S

G

G

G

N
|
N

K
|
K

I

I

R

R

K

K

L

L

E

E

F

Y

S

L

A

L

A

G

E

D

A

A

L

L

N

S

K

K

G

G

C

A

N

D

T

V

L

V

I

I

T

T

C

V

G

G

G

A

I

V

Q

H

S
|
S

N
|
N

H
|
H

C

A

R

F

A

V

T

T

A

G

A

L

V

A

A

A

V

K

K

K

L

L

G

G

F

L

K

D

C

A

C

I

L

L

V

V

L

L

N

R

G

G

S

K

N

E

D

-

T

-

E

E

V

L

D

K

G

G

N

N

L

Y

L

L

D

D

K

K

L

I

L

M

G

G

A

I

E

E

I

T

Y

R

F

V

V

Y

S

D

Q

A

K

K

E

D

Y

-

E

-

N

-

R

-

R

S

M

F

E

E

I

L

M

M

K

K

-

Y

-

A

-

E

E

E

I

I

K

A

T

E

N

E

M

L

E

K

N

R

K

E

G

G

L

R

K

K

P

P

Y

Y

I

V

I

I

P

P

E

P

G

G

A

G

S

A

N

S

G

P

I

I

G

G

G

T

F

L

G

G

Y

Y

Y

V

K

R

A

A

V

V

Q

G

E

E

I

I

M

A

L

T

Q

Q

E

S

R

-

E

-

M

-

K

E

V

V

H

K

F

F

D

D

G

S

I

I

V

V

I

V

A

A

T
x
A

G
|
G

S
|
S

G
|
G

T
|
T

Y

L

S

A

G

G

L

L

L

S

L

L

G

G

S

L

R

S

I

I

L

L

N

N

Y

E

D

D

A

I

K

R

I

P

Y

V

G

G

V

I

N

A

V

V

C

G

Q

R

N

F

E

G

K

E

Y

V

F

M

K

T

D

S

R

K

I

L

Y

D

E

N

I

L

L

I

H

K

D

E

S

A

M

A

K

E

Y

L

I

L

D

G

V

V

N

K

L

V

N

E

F

V

S

R

K

P

D

E

E

L

I

Y

N

D

I

Y

I

S

D

F

G

G

Y

E

V
x
Y

G

G

R

K

G

-

Y

-

A

-

L

-

S

I

R

T

E

G

E

E

E

V

L

A

E

Q

F

I

I

I

K

R

E

K

L

V

A

G

E

T

L

R

E

E

G

G

I

I

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

F

Y

Y

G

G

L

L

T

V

Q

D

E

L

I

A

K

R

K

K

G

G

K

E

F

L

S

G

K

-

Y

-

K

E

N

K

L

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

I

I

F

S

G

G

I

T

F

F

active site: K54 (= K48), Y256 (≠ V256), Y282 (= Y286)
binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: N53 (= N47), K54 (= K48), S81 (= S75), N82 (= N76), H83 (= H77), A189 (≠ T189), G190 (= G190), S191 (= S191), G192 (= G192), G193 (= G193), T194 (= T194), Y282 (= Y286), T308 (= T314), G309 (= G315), G310 (= G316)

1j0aA Crystal structure analysis of the acc deaminase homologue (see paper)
40% identity, 98% coverage: 1:321/329 of query aligns to 9:315/325 of 1j0aA

query
sites
1j0aA

M

L

I

A

K

K

I

F

P

P

E

-

R

R

M

V

N

E

M

L

A

I

N

P

L

W

P

E

T

T

K

P

I

I

E

Q

K

Y

M

L

E

P

R

N

L

I

S

S

Q

R

K

E

L

I

G

G

G

A

P

-

D

D

I

V

Y

Y

I

I

K

K

R

R

D

D

Q

L

T

T

G

G

T

L

E

G

I

I

S

G

G

G

N
|
N

K
|
K

I

I

R

R

K
|
K

L

L

E

E

F

Y

S

L

A

L

A

G

E

D

A

A

L

L

N

S

K

K

G

G

C

A

N

D

T

V

L

V

I

I

T

T

C

V

G

G

G

A

I

V

Q

H

S

S

N
|
N

H

H

C

A

R

F

A

V

T

T

A

G

A

L

V

A

A

A

V

K

K

K

L

L

G

G

F

L

K

D

C

A

C

I

L

L

V

V

L

L

N

R

G

G

S

K

N

E

D

-

T

-

E

E

V

L

D

K

G

G

N

N

L

Y

L

L

D

D

K

K

L

I

L

M

G

G

A

I

E

E

I

T

Y

R

F

V

V

Y

S

D

Q

A

K

K

E

D

Y

-

E

-

N

-

R

-

R

S

M

F

E

E

I

L

M

M

K

K

-

Y

-

A

-

E

E

E

I

I

K

A

T

E

N

E

M

L

E

K

N

R

K

E

G

G

L

R

K

K

P

P

Y

Y

I

V

I

I

P

P

E

P

G

G

A

G

S

A

N

S

G

P

I

I

G

G

G

T

F

L

G

G

Y

Y

Y

V

K

R

A

A

V

V

Q

G

E

E

I

I

M

A

L

T

Q

Q

E

S

R

-

E

-

M

-

K

E

V

V

H

K

F

F

D

D

G

S

I

I

V

V

I

V

A

A

T

A

G
|
G

S
|
S

G
|
G

T
|
T

Y

L

S

A

G

G

L

L

L

S

L

L

G

G

S

L

R

S

I

I

L

L

N

N

Y

E

D

D

A

I

K

R

I

P

Y

V

G

G

V

I

N

A

V

V

C

G

Q

R

N

F

E

G

K

E

Y

V

F

M

K

T

D

S

R

K

I

L

Y

D

E

N

I

L

L

I

H

K

D

E

S

A

M

A

K

E

Y

L

I

L

D

G

V

V

N

K

L

V

N

E

F

V

S

R

K

P

D

E

E

L

I

Y

N

D

I

Y

I

S

D

F

G

G

Y

E

V
x
Y

G

G

R

K

G

-

Y

-

A

-

L

-

S

I

R

T

E

G

E

E

E

V

L

A

E

Q

F

I

I

I

K

R

E

K

L

V

A

G

E

T

L

R

E

E

G

G

I

I

L

L

D

D

P

P

V

V

Y
|
Y

T

T

G

G

K

K

A

A

M

F

Y

Y

G

G

L

L

T

V

Q

D

E

L

I

A

K

R

K

K

G

G

K

E

F

L

S

G

K

-

Y

-

K

E

N

K

L

I

L

L

F

F

I

I

H

H

T
|
T

G
|
G

I

I

F

S

G

G

I

T

F

F

active site: K54 (= K48), Y256 (≠ V256), Y282 (= Y286)
binding pyridoxal-5'-phosphate: N53 (= N47), K54 (= K48), K57 (= K51), N82 (= N76), G190 (= G190), S191 (= S191), G192 (= G192), G193 (= G193), T194 (= T194), Y282 (= Y286), T308 (= T314), G309 (= G315), G310 (= G316)

1tzmA Crystal structure of acc deaminase complexed with substrate analog b- chloro-d-alanine (see paper)
33% identity, 92% coverage: 14:317/329 of query aligns to 15:318/331 of 1tzmA

query
sites
1tzmA

P

P

T

T

K

P

I

I

E

Q

K

P

M

L

E

A

R

R

L

L

S

S

Q

K

K

H

L

L

G

G

P

K

-

V

D

H

I

L

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N
|
N

K
|
K

I

T

R

R

K
|
K

L

L

E

E

F

Y

S

L

A

I

A

P

E

E

A

A

L

L

N

A

K

Q

G

G

C

C

N

D

T

T

L

L

I

V

T

S

C

I

G
|
G

G

G

I

I

Q

Q

S
|
S

N
|
N

H
x
Q

C

T

R

R

A

Q

T

V

A

A

V

V

A

A

V

A

K

H

L

L

G

G

F

M

K

K

C

C

C

V

L

L

V

V

L

Q

N

E

G

N

S
x
W

-

V

-

N

-

Y

N

S

D

D

T

A

E

V

V

Y

D

D

-

R

-

V

G

G

N

N

L

I

L

Q

L

M

D

S

K

R

L

I

L

L

G

G

A

A

E

D

I

V

Y

R

F

L

V

V

S

P

Q

D

K

R

E

S

Y

W

E

E

N

D

R

-

M

-

E

-

I

A

M

L

K

E

E

S

I

V

K

R

T

A

N

-

M

-

E

-

N

-

K

A

G

G

L

G

K

K

P

P

Y

Y

I

A

I

I

P

P

E
x
A

G
|
G

A

C

S

S

N

D

-

H

G

P

I

L

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

E

E

E

I

V

M

R

L

A

Q

Q

E

E

R

A

E

E

M

L

K

G

V

F

H

K

F

F

D

D

G

Y

I

V

V

V

I

V

A
x
C

T
x
S

G
x
V

S
x
T

G
|
G

G

S

T
|
T

Y

Q

S

A

G

G

L

M

L

V

G

G

S

F

R

A

I

A

L

D

N

G

Y

R

D

A

A

D

K

R

I

V

Y

I

G

G

V

V

N

D

V

A

C

S

Q

A

N

K

E

P

K

A

Y

Q

F

T

K

R

D

E

R

Q

I

I

Y

T

E

R

I

I

L

A

H

R

D

Q

S

T

M

A

K

E

Y

K

I

V

D

G

V

L

N

E

L

R

N

D

F

I

S

M

K

R

D

A

E

D

I

V

N

V

I

L

I

D

D

E

G

R

Y

F

V

A

G

G

R

P

G

E

Y
|
Y

A

G

L

L

S

P

R

N

E

E

E

G

E

T

L

L

E

E

F

A

I

I

K

R

E

L

L

C

A

A

E

R

L

T

E

E

G

G

I

M

I

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

Y

H

G

G

L

M

T

I

Q

E

E

M

I

V

K

R

K

N

G

G

K

E

F

F

S

P

K

E

Y

G

K

S

N

R

L

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

I

V

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding 3-chloro-D-alanine: G74 (= G71), S78 (= S75), N79 (= N76), Q80 (≠ H77), W102 (≠ S99), A153 (≠ E153), G154 (= G154), Y287 (= Y286)
binding amino-acrylate: K51 (= K48), S78 (= S75), Q80 (≠ H77), Y287 (= Y286)
binding pyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), C189 (≠ A188), S190 (≠ T189), V191 (≠ G190), T192 (≠ S191), G193 (= G192), T195 (= T194), Y287 (= Y286), E288 (≠ T287), L315 (≠ T314), G316 (= G315), G317 (= G316)

1tzkA Crystal structure of 1-aminocyclopropane-1-carboxylate-deaminase complexed with alpha-keto-butyrate (see paper)
33% identity, 92% coverage: 14:317/329 of query aligns to 15:318/331 of 1tzkA

query
sites
1tzkA

P

P

T

T

K

P

I

I

E

Q

K

P

M

L

E

A

R

R

L

L

S

S

Q

K

K

H

L

L

G

G

P

K

-

V

D

H

I

L

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N
|
N

K
|
K

I

T

R

R

K
|
K

L

L

E

E

F

Y

S

L

A

I

A

P

E

E

A

A

L

L

N

A

K

Q

G

G

C

C

N

D

T

T

L

L

I

V

T

S

C

I

G
|
G

G

G

I

I

Q

Q

S
|
S

N
|
N

H
x
Q

C

T

R

R

A

Q

T

V

A

A

V

V

A

A

V

A

K

H

L

L

G

G

F

M

K

K

C

C

C

V

L

L

V

V

L

Q

N

E

G

N

S

W

-

V

-

N

-

Y

N

S

D

D

T

A

E

V

V

Y

D

D

-

R

-

V

G

G

N

N

L

I

L

Q

L

M

D

S

K

R

L

I

L

L

G

G

A

A

E

D

I

V

Y

R

F

L

V

V

S

P

Q

D

K

R

E

S

Y

W

E

E

N

D

R

-

M

-

E

-

I

A

M

L

K

E

E

S

I

V

K

R

T

A

N

-

M

-

E

-

N

-

K

A

G

G

L

G

K

K

P

P

Y

Y

I

A

I

I

P

P

E
x
A

G
|
G

A

C

S

S

N

D

-

H

G

P

I

L

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

E

E

E

I

V

M

R

L

A

Q

Q

E

E

R

A

E

E

M

L

K

G

V

F

H

K

F

F

D

D

G

Y

I

V

V

V

I

V

A
x
C

T
x
S

G
x
V

S
x
T

G
|
G

G

S

T
|
T

Y

Q

S

A

G

G

L

M

L

V

G

G

S

F

R

A

I

A

L

D

N

G

Y

R

D

A

A

D

K

R

I

V

Y

I

G

G

V

V

N

D

V

A

C

S

Q

A

N

K

E

P

K

A

Y

Q

F

T

K

R

D

E

R

Q

I

I

Y

T

E

R

I

I

L

A

H

R

D

Q

S

T

M

A

K

E

Y

K

I

V

D

G

V

L

N

E

L

R

N

D

F

I

S

M

K

R

D

A

E

D

I

V

N

V

I

L

I

D

D

E

G

R

Y

F

V

A

G

G

R

P

G

E

Y
|
Y

A

G

L

L

S

P

R

N

E

E

E

G

E

T

L

L

E

E

F

A

I

I

K

R

E

L

L

C

A

A

E

R

L

T

E

E

G

G

I

M

I

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

Y

H

G

G

L

M

T

I

Q

E

E

M

I

V

K

R

K

N

G

G

K

E

F

F

S

P

K

E

Y

G

K

S

N

R

L

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

I

V

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding 2-ketobutyric acid: K51 (= K48), G74 (= G71), S78 (= S75), N79 (= N76), Q80 (≠ H77), A153 (≠ E153), G154 (= G154), Y287 (= Y286)
binding pyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), C189 (≠ A188), S190 (≠ T189), V191 (≠ G190), T192 (≠ S191), G193 (= G192), T195 (= T194), Y287 (= Y286), E288 (≠ T287), L315 (≠ T314), G316 (= G315), G317 (= G316)

1tzjA Crystal structure of 1-aminocyclopropane-1-carboxylate deaminase complexed with d-vinyl glycine (see paper)
33% identity, 92% coverage: 14:317/329 of query aligns to 15:318/331 of 1tzjA

query
sites
1tzjA

P

P

T

T

K

P

I

I

E

Q

K

P

M

L

E

A

R

R

L

L

S

S

Q

K

K

H

L

L

G

G

P

K

-

V

D

H

I

L

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N
|
N

K
|
K

I

T

R

R

K
|
K

L

L

E

E

F

Y

S

L

A

I

A

P

E

E

A

A

L

L

N

A

K

Q

G

G

C

C

N

D

T

T

L

L

I

V

T

S

C

I

G
|
G

G

G

I

I

Q

Q

S
|
S

N
|
N

H
x
Q

C

T

R

R

A

Q

T

V

A

A

V

V

A

A

V

A

K

H

L

L

G

G

F

M

K

K

C

C

C

V

L

L

V

V

L

Q

N

E

G

N

S

W

-

V

-

N

-

Y

N

S

D

D

T

A

E

V

V

Y

D

D

-

R

-

V

G

G

N

N

L

I

L

Q

L

M

D

S

K

R

L

I

L

L

G

G

A

A

E

D

I

V

Y

R

F

L

V

V

S

P

Q

D

K

R

E

S

Y

W

E

E

N

D

R

-

M

-

E

-

I

A

M

L

K

E

E

S

I

V

K

R

T

A

N

-

M

-

E

-

N

-

K

A

G

G

L

G

K

K

P

P

Y

Y

I

A

I

I

P

P

E
x
A

G
|
G

A

C

S

S

N

D

-

H

G

P

I

L

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

E

E

E

I

V

M

R

L

A

Q

Q

E

E

R

A

E

E

M

L

K

G

V

F

H

K

F

F

D

D

G

Y

I

V

V

V

I

V

A
x
C

T
x
S

G
x
V

S
x
T

G
|
G

G

S

T
|
T

Y

Q

S

A

G

G

L

M

L

V

G

G

S

F

R

A

I

A

L

D

N

G

Y

R

D

A

A

D

K

R

I

V

Y

I

G

G

V

V

N

D

V

A

C

S

Q

A

N

K

E

P

K

A

Y

Q

F

T

K

R

D

E

R

Q

I

I

Y

T

E

R

I

I

L

A

H

R

D

Q

S

T

M

A

K

E

Y

K

I

V

D

G

V

L

N

E

L

R

N

D

F

I

S

M

K

R

D

A

E

D

I

V

N

V

I

L

I

D

D

E

G

R

Y

F

V

A

G

G

R

P

G

E

Y
|
Y

A

G

L

L

S

P

R

N

E

E

E

G

E

T

L

L

E

E

F

A

I

I

K

R

E

L

L

C

A

A

E

R

L

T

E

E

G

G

I

M

I

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

Y

H

G

G

L

M

T

I

Q

E

E

M

I

V

K

R

K

N

G

G

K

E

F

F

S

P

K

E

Y

G

K

S

N

R

L

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

I

V

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding d-vinylglycine: G74 (= G71), S78 (= S75), N79 (= N76), Q80 (≠ H77), A153 (≠ E153), G154 (= G154), Y287 (= Y286)
binding pyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), C189 (≠ A188), S190 (≠ T189), V191 (≠ G190), T192 (≠ S191), G193 (= G192), T195 (= T194), Y287 (= Y286), E288 (≠ T287), L315 (≠ T314), G316 (= G315), G317 (= G316)

1tz2A Crystal structure of 1-aminocyclopropane-1-carboyxlate deaminase complexed with acc (see paper)
33% identity, 92% coverage: 14:317/329 of query aligns to 15:318/331 of 1tz2A

query
sites
1tz2A

P

P

T

T

K

P

I

I

E

Q

K

P

M

L

E

A

R

R

L

L

S

S

Q

K

K

H

L

L

G

G

P

K

-

V

D

H

I

L

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N
|
N

K
|
K

I

T

R

R

K
|
K

L

L

E

E

F

Y

S

L

A

I

A

P

E

E

A

A

L

L

N

A

K

Q

G

G

C

C

N

D

T

T

L

L

I

V

T

S

C

I

G

G

I

I

Q

Q

S
|
S

N
|
N

H

Q

C

T

R

R

A

Q

T

V

A

A

V

V

A

A

V

A

K

H

L

L

G

G

F

M

K

K

C

C

C

V

L

L

V

V

L

Q

N

E

G

N

S

W

-

V

-

N

-

Y

N

S

D

D

T

A

E

V

V

Y

D

D

-

R

-

V

G

G

N

N

L

I

L

Q

L

M

D

S

K

R

L

I

L

L

G

G

A

A

E

D

I

V

Y

R

F

L

V

V

S

P

Q

D

K

R

E

S

Y

W

E

E

N

D

R

-

M

-

E

-

I

A

M

L

K

E

E

S

I

V

K

R

T

A

N

-

M

-

E

-

N

-

K

A

G

G

L

G

K

K

P

P

Y

Y

I

A

I

I

P

P

E

A

G

G

A

C

S

S

N

D

-

H

G

P

I

L

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

E

E

E

I

V

M

R

L

A

Q

Q

E

E

R

A

E

E

M

L

K

G

V

F

H

K

F

F

D

D

G

Y

I

V

V

V

I

V

A
x
C

T
x
S

G
x
V

S
x
T

G
|
G

G

S

T
|
T

Y

Q

S

A

G

G

L

M

L

V

G

G

S

F

R

A

I

A

L

D

N

G

Y

R

D

A

A

D

K

R

I

V

Y

I

G

G

V

V

N

D

V

A

C

S

Q

A

N

K

E

P

K

A

Y

Q

F

T

K

R

D

E

R

Q

I

I

Y

T

E

R

I

I

L

A

H

R

D

Q

S

T

M

A

K

E

Y

K

I

V

D

G

V

L

N

E

L

R

N

D

F

I

S

M

K

R

D

A

E

D

I

V

N

V

I

L

I

D

D

E

G

R

Y

F

V

A

G

G

R

P

G

E

Y
|
Y

A

G

L

L

S

P

R

N

E

E

E

G

E

T

L

L

E

E

F

A

I

I

K

R

E

L

L

C

A

A

E

R

L

T

E

E

G

G

I

M

I

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

Y

H

G

G

L

M

T

I

Q

E

E

M

I

V

K

R

K

N

G

G

K

E

F

F

S

P

K

E

Y

G

K

S

N

R

L

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

I

V

active site: K51 (= K48), Y261 (= Y260), Y287 (= Y286)
binding 1-aminocyclopropanecarboxylic acid: K51 (= K48), S78 (= S75), Y287 (= Y286)
binding pyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), C189 (≠ A188), S190 (≠ T189), V191 (≠ G190), T192 (≠ S191), G193 (= G192), T195 (= T194), Y287 (= Y286), E288 (≠ T287), L315 (≠ T314), G316 (= G315), G317 (= G316)

1rqxC Crystal structure of acc deaminase complexed with inhibitor (see paper)
33% identity, 92% coverage: 14:317/329 of query aligns to 15:325/338 of 1rqxC

query
sites
1rqxC

P

P

T

T

K

P

I

I

E

Q

K

P

M

L

E

A

R

R

L

L

S

S

Q

K

K

H

L

L

G

G

P

K

-

V

D

H

I

L

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N
|
N

K
|
K

I

T

R

R

K
|
K

L

L

E

E

F

Y

S

L

A

I

A

P

E

E

A

A

L

L

N

A

K

Q

G

G

C

C

N

D

T

T

L

L

I

V

T

S

C

I

G

G

I

I

Q

Q

S
|
S

N
|
N

H
x
Q

C

T

R

R

A

Q

T

V

A

A

V

V

A

A

V

A

K

H

L

L

G

G

F

M

K

K

C

C

C

V

L

L

V

V

L

Q

N

E

G

N

S

W

-

V

-

N

-

Y

N

S

D

D

T

A

E

V

V

Y

D

D

-

R

-

V

G

G

N

N

L

I

L

Q

L

M

D

S

K

R

L

I

L

L

G

G

A

A

E

D

I

V

Y

R

F

L

V

V

S

P

Q

D

K

G

E

-

Y

F

E

D

N

I

R

G

R

F

M

R

E

R

I

S

M

W

K

E

E

D

I

A

K

L

T

E

N

S

M

V

E

R

N

A

K

A

G

G

L

G

K

K

P

P

Y

Y

I

A

I

I

P

P

E

A

G

G

A

C

S

S

N

D

-

H

G

P

I

L

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

E

E

E

I

V

M

R

L

A

Q

Q

E

E

R

A

E

E

M

L

K

G

V

F

H

K

F

F

D

D

G

Y

I

V

V

V

I

V

A

C

T
x
S

G
x
V

S
x
T

G
|
G

G

S

T
|
T

Y

Q

S

A

G

G

L

M

L

V

G

G

S

F

R

A

I

A

L

D

N

G

Y

R

D

A

A

D

K

R

I

V

Y

I

G

G

V

V

N

D

V

A

C

S

Q

A

N

K

E

P

K

A

Y

Q

F

T

K

R

D

E

R

Q

I

I

Y

T

E

R

I

I

L

A

H

R

D

Q

S

T

M

A

K

E

Y

K

I

V

D

G

V

L

N

E

L

R

N

D

F

I

S

M

K

R

D

A

E

D

I

V

N

V

I

L

I

D

D

E

G

R

Y

F

V

A

G

G

R

P

G

E

Y
|
Y

A

G

L

L

S

P

R

N

E

E

E

G

E

T

L

L

E

E

F

A

I

I

K

R

E

L

L

C

A

A

E

R

L

T

E

E

G

G

I

M

I

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

Y

H

G

G

L

M

T

I

Q

E

E

M

I

V

K

R

K

N

G

G

K

E

F

F

S

P

K

E

Y

G

K

S

N

R

L

V

L

L

F

Y

I

A

H

H

T
x
L

G
|
G

I

V

active site: K51 (= K48), Y268 (= Y260), Y294 (= Y286)
binding 1-aminocyclopropylphosphonate: K51 (= K48), S78 (= S75), N79 (= N76), Q80 (≠ H77), T199 (≠ S191), Y294 (= Y286)
binding pyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), S197 (≠ T189), V198 (≠ G190), T199 (≠ S191), G200 (= G192), T202 (= T194), Y294 (= Y286), E295 (≠ T287), L322 (≠ T314), G323 (= G315), G324 (= G316)

Q5PWZ8 1-aminocyclopropane-1-carboxylate deaminase; ACC deaminase; ACCD; EC 3.5.99.7 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see paper)
33% identity, 95% coverage: 6:316/329 of query aligns to 7:324/338 of Q5PWZ8

query
sites
Q5PWZ8

E

E

R

R

M

Y

N

P

M

L

A

T

N

F

L

G

P

P

T

S

K

P

I

I

E

T

K

P

M

L

E

K

R

R

L

L

S

S

Q

E

K

H

L

L

G

G

P

K

-

V

D

E

I

L

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G
|
G

T

L

E

A

I

F

S

G

G

G

N

N

K

K

I

T

R

R

K

K

L

L

E

E

F

Y

S

L

A

I

A

P

E

E

A

A

L

I

N

E

K

Q

G

G

C

C

N

D

T

T

L

L

I

V

T

S

C

I

G

G

I

I

Q

Q

S

S

N

N

H

Q

C

T

R

R

A

Q

T

V

A

A

V

V

A

A

V

A

K

H

L

L

G

G

F

M

K

K

C

C

C

V

L

L

V

V

L

Q

N

E

G

N

S

W

-

V

-

N

-

Y

N

S

D

D

T

A

E

V

V

Y

D

D

-

R

-

V

G

G

N

N

L

I

L

E

L

M

D

S

K

R

L

I

L

M

G

G

A

A

E

D

I

V

Y

R

F

L

V

D

S

A

Q

A

K

G

E

F

Y

D

E

I

N

G

R

I

R

R

M

P

E

S

I

W

M

E

K

K

E

A

I

M

K

S

T

D

N

V

M

V

E

E

N

-

K

R

G

G

L

G

K

K

P

P

Y

F

I

P

I

I

P

P

E

A

G

G

A

C

S

S

-

E

N

H

G

P

I

Y

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

E

E

E

I

V

M

R

L

Q

Q

Q

E

E

R

K

E

E

M

L

K

G

V

F

H

K

F

F

D

D

G

Y

I

I

V

V

I

V

A

C

T

S

G

V

S

T

G

G

G

S

T

T

Y

Q

S

A

G

G

L

M

L

V

G

G

S

F

R

A

I

A

L

D

N

G

Y

R

D

S

A

K

K

N

I

V

Y

I

G

G

V

V

N

D

V

A

C

S

Q

A

N

K

E

P

K

E

Y

Q

F

T

K

K

D

A

R

Q

I

I

Y

L

E

R

I

I

L

A

H

R

D

H

S

T

M

A

K

E

Y

L

I

V

D

E

V

L

N

G

L

R

N

E

F

I

S

T

K

E

D

E

E

D

I

V

N

V

I

L

I

D

D

T

G

R

Y

F

V

A

G

Y

R

P

G

E

Y

Y

A

G

L

L

S

P

R

N

E

E

E

G

E

T

L

L

E

E

F

A

I

I

K

R

E

L

L

C

A

G

E

S

L

L

E

E

G

G

I

V

I

L

L

T

D

D

P

P

V

V

Y

Y

T

E

G

G

K

K

A

S

M

M

Y

H

G

G

L

M

T

I

Q

E

E

M

I

V

K

R

G

G

K

E

F

F

S

P

K

D

Y

G

K

S

N

K

L

V

L

L

F

Y

I

A

H

H

T

L

G

G

G44 (= G41) mutation to D: Loss of activity.

1f2dA 1-aminocyclopropane-1-carboxylate deaminase (see paper)
32% identity, 95% coverage: 14:325/329 of query aligns to 15:340/341 of 1f2dA

query
sites
1f2dA

P

P

T

S

K

P

I

I

E

S

K

N

M

L

E

N

R

R

L

L

S

S

Q

Q

K

H

L

L

G

G

G

S

P

K

-

V

D

N

I

V

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N
|
N

K
|
K

I

L

R

R

K
|
K

L

L

E

E

F

Y

S

I

A

V

A

P

E

D

A

I

L

V

N

E

K

G

G

D

C

Y

N

T

T

H

L

L

I

V

T

S

C

I

G

G

I

R

Q

Q

S

S

N
|
N

H

Q

C

T

R

R

A

M

T

V

A

A

V

L

A

A

V

A

K

K

L

L

G

G

F

K

K

K

C

C

C

V

L

L

V

I

L

Q

N

E

G

D

S

W

-

V

-

P

-

I

N

P

D

E

T

A

E

E

V

K

D

D

-

V

-

Y

-

N

-

R

-

V

G

G

N

N

L

I

L

E

L

L

D

S

K

R

L

I

L

M

G

G

A

A

E

D

I

V

Y

R

F

V

V

I

S

E

Q

D

K

G

E

F

Y

D

E

I

N

G

R

M

R

R

M

K

E

S

I

F

M

A

K

N

E

A

I

L

K

Q

T

-

N

E

M

L

E

E

N

D

K

A

G

G

L

H

K

K

P

P

Y

Y

I

P

I

I

P

P

E

A

G

G

A

C

S

S

-

E

N

H

G

K

I

Y

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

D

E

E

I

V

M

I

L

N

Q

Q

E

E

R

V

E

E

M

L

K

G

V

I

H

K

F

F

D

D

G

K

I

I

V

V

I

V

A

C

T
x
C

G

V

S
x
T

G
|
G

G
x
S

T
|
T

Y

T

S

A

G

G

L

I

L

L

L

A

G

G

S

M

R

A

I

Q

L

Y

N

G

Y

R

D

Q

A

D

K

D

I

V

Y

I

G

A

V

I

N

D

V

A

C

S

Q

F

N

T

E

S

K

E

Y

K

F

T

K

K

D

E

R

Q

I

T

Y

L

E

R

I

I

L

A

H

N

D

N

S

T

M

A

K

K

Y

L

I

I

D

G

V

V

N

E

L

H

N

E

F

F

S

K

K

D

D

F

E

T

I

L

N

D

I

T

I

R

D

F

G

A

Y

Y

V

-

G

-

R

P

G

C

Y
|
Y

A

G

L

V

S

P

R

N

E

E

E

G

E

T

L

I

E

E

F

A

I

I

K

R

E

T

L

C

A

A

E

E

L

Q

E

E

G

G

I

V

I

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

Y

Q

G

G

L

L

T

I

Q

A

E

L

I

I

K

K

K

E

G

D

K

Y

F

F

S

K

K

P

Y

G

K

A

N

N

L

V

L

L

F

Y

I

V

H

H

T
x
L

G
|
G

I

A

F

P

G

A

I

L

-

S

-

A

-

Y

-

S

-

F

F

F

P

P

Q

T

K

K

S

T

active site: K51 (= K48), Y269 (= Y260), Y295 (= Y286)
binding pyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), C200 (≠ T189), T202 (≠ S191), G203 (= G192), S204 (≠ G193), T205 (= T194), Y295 (= Y286), E296 (≠ T287), L323 (≠ T314), G324 (= G315), G325 (= G316)

Q7M523 1-aminocyclopropane-1-carboxylate deaminase; ACC deaminase; ACCD; EC 3.5.99.7 from Cyberlindnera saturnus (Yeast) (Williopsis saturnus) (see paper)
32% identity, 95% coverage: 14:325/329 of query aligns to 15:340/341 of Q7M523

query
sites
Q7M523

P

P

T

S

K

P

I

I

E

S

K

N

M

L

E

N

R

R

L

L

S

S

Q

Q

K

H

L

L

G

G

G

S

P

K

-

V

D

N

I

V

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N

N

K

K

I

L

R

R

K

K

L

L

E

E

F

Y

S

I

A

V

A

P

E

D

A

I

L

V

N

E

K

G

G

D

C

Y

N

T

T

H

L

L

I

V

T

S

C

I

G

G

I

R

Q

Q

S

S

N

N

H

Q

C

T

R

R

A

M

T

V

A

A

V

L

A

A

V

A

K

K

L

L

G

G

F

K

K

K

C

C

C

V

L

L

V

I

L

Q

N

E

G

D

S

W

-

V

-

P

-

I

N

P

D

E

T

A

E

E

V

K

D

D

-

V

-

Y

-

N

-

R

-

V

G

G

N

N

L

I

L

E

L

L

D

S

K

R

L

I

L

M

G

G

A

A

E

D

I

V

Y

R

F

V

V

I

S

E

Q

D

K

G

E

F

Y

D

E

I

N

G

R

M

R

R

M

K

E

S

I

F

M

A

K

N

E

A

I

L

K

Q

T

-

N

E

M

L

E

E

N

D

K

A

G

G

L

H

K

K

P

P

Y

Y

I

P

I

I

P

P

E

A

G

G

A

C

S

S

-

E

N

H

G

K

I

Y

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

D

E

E

I

V

M

I

L

N

Q

Q

E

E

R

V

E

E

M

L

K

G

V

I

H

K

F

F

D

D

G

K

I

I

V

V

I

V

A

C

T

C

G

V

S

T

G

G

G

S

T

T

Y

T

S

A

G

G

L

I

L

L

L

A

G

G

S

M

R

A

I

Q

L

Y

N

G

Y

R

D

Q

A

D

K

D

I

V

Y

I

G

A

V

I

N

D

V

A

C

S

Q

F

N

T

E

S

K

E

Y

K

F

T

K

K

D

E

R

Q

I

T

Y

L

E

R

I

I

L

A

H

N

D

N

S

T

M

A

K

K

Y

L

I

I

D

G

V

V

N

E

L

H

N

E

F

F

S

K

K

D

D

F

E

T

I

L

N

D

I

T

I

R

D

F

G

A

Y

Y

V

-

G

-

R

P

G

C

Y

Y

A

G

L

V

S

P

R

N

E

E

E

G

E

T

L

I

E

E

F

A

I

I

K

R

E

T

L

C

A

A

E

E

L

Q

E

E

G

G

I

V

I

L

L

T

D

D

P

P

V

V

Y

Y

T

E

G

G

K

K

A

S

M

M

Y

Q

G

G

L

L

T

I

Q

A

E

L

I

I

K

K

K

E

G

D

K

Y

F

F

S

K

K

P

Y

G

K

A

N

N

L

V

L

L

F

Y

I

V

H

H

T

L

G

G

I

A

F

P

G

A

I

L

-

S

-

A

-

Y

-

S

-

F

F

F

P

P

Q

T

K

K

S

T

Sites not aligning to the query:

1 modified: N-acetylserine

1j0eA Acc deaminase mutant reacton intermediate (see paper)
31% identity, 95% coverage: 14:325/329 of query aligns to 15:340/341 of 1j0eA

query
sites
1j0eA

P

P

T

S

K

P

I

I

E

S

K

N

M

L

E

N

R

R

L

L

S

S

Q

Q

K

H

L

L

G

G

G

S

P

K

-

V

D

N

I

V

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N
|
N

K
|
K

I

L

R

R

K
|
K

L

L

E

E

F

Y

S

I

A

V

A

P

E

D

A

I

L

V

N

E

K

G

G

D

C

Y

N

T

T

H

L

L

I

V

T

S

C

I

G

G

I

R

Q

Q

S
|
S

N
|
N

H
x
Q

C

T

R

R

A

M

T

V

A

A

V

L

A

A

V

A

K

K

L

L

G

G

F

K

K

K

C

C

C

V

L

L

V

I

L

Q

N

E

G

D

S

W

-

V

-

P

-

I

N

P

D

E

T

A

E

E

V

K

D

D

-

V

-

Y

-

N

-

R

-

V

G

G

N

N

L

I

L

E

L

L

D

S

K

R

L

I

L

M

G

G

A

A

E

D

I

V

Y

R

F

V

V

I

S

E

Q

D

K

G

E

F

Y

D

E

I

N

G

R

M

R

R

M

K

E

S

I

F

M

A

K

N

E

A

I

L

K

Q

T

-

N

E

M

L

E

E

N

D

K

A

G

G

L

H

K

K

P

P

Y

Y

I

P

I

I

P

P

E

A

G

G

A

C

S

S

-

E

N

H

G

K

I

Y

G

G

F

L

G

G

Y

F

Y

V

K

G

A

F

V

A

Q

D

E

E

I

V

M

I

L

N

Q

Q

E

E

R

V

E

E

M

L

K

G

V

I

H

K

F

F

D

D

G

K

I

I

V

V

I

V

A
x
C

T

C

G

V

S
x
T

G
|
G

G

S

T
|
T

Y

T

S

A

G

G

L

I

L

L

L

A

G

G

S

M

R

A

I

Q

L

Y

N

G

Y

R

D

Q

A

D

K

D

I

V

Y

I

G

A

V

I

N

D

V

A

C

S

Q

F

N

T

E

S

K

E

Y

K

F

T

K

K

D

E

R

Q

I

T

Y

L

E

R

I

I

L

A

H

N

D

N

S

T

M

A

K

K

Y

L

I

I

D

G

V

V

N

E

L

H

N

E

F

F

S

K

K

D

D

F

E

T

I

L

N

D

I

T

I

R

D

F

G

A

Y

Y

V

-

G

-

R

P

G

C

Y

Y

A

G

L

V

S

P

R

N

E

E

E

G

E

T

L

I

E

E

F

A

I

I

K

R

E

T

L

C

A

A

E

E

L

Q

E

E

G

G

I

V

I

L

L

T

D

D

P

P

V

V

Y
x
F

T
x
E

G

G

K

K

A

S

M

M

Y

Q

G

G

L

L

T

I

Q

A

E

L

I

I

K

K

K

E

G

D

K

Y

F

F

S

K

K

P

Y

G

K

A

N

N

L

V

L

L

F

Y

I

V

H

H

T

L

G

G

I

A

F

P

G

A

I

L

-

S

-

A

-

Y

-

S

-

F

F

F

P

P

Q

T

K

K

S

T

binding 1-aminocyclopropanecarboxylic acid: S78 (= S75), N79 (= N76), Q80 (≠ H77), F295 (≠ Y286)
binding pyridoxal-5'-phosphate: N50 (= N47), K51 (= K48), K54 (= K51), N79 (= N76), C199 (≠ A188), T202 (≠ S191), G203 (= G192), T205 (= T194), F295 (≠ Y286), E296 (≠ T287)

1j0dA Acc deaminase mutant complexed with acc (see paper)
31% identity, 95% coverage: 14:325/329 of query aligns to 15:340/341 of 1j0dA

query
sites
1j0dA

P

P

T

S

K

P

I

I

E

S

K

N

M

L

E

N

R

R

L

L

S

S

Q

Q

K

H

L

L

G

G

G

S

P

K

-

V

D

N

I

V

Y

Y

I

A

K

K

R

R

D

E

D

D

-

C

Q

N

T

S

G

G

T

L

E

A

I

F

S

G

G

G

N

N

K

T

I

L

R

R

K

K

L

L

E

E

F

Y

S

I

A

V

A

P

E

D

A

I

L

V

N

E

K

G

G

D

C

Y

N

T

T

H

L

L

I

V

T

S

C

I

G

G

I

R

Q

Q

S
|
S

N
|
N

H
x
Q

C

T

R

R

A

M

T

V

A

A

V

L

A

A

V

A

K

K

L

L

G

G

F

K

K

K

C

C

C

V

L

L

V

I

L

Q

N

E

G

D

S

W

-

V

-

P

-

I

N

P

D

E

T

A

E

E

V

K

D

D

-

V

-

Y

-

N

-

R

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V

G

G

N

N

L

I

L

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L

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D

S

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G

A

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N

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-

N

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M

L

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N

D

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A

G

G

L

H

K

K

P

P

Y

Y

I

P

I

I

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P

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x
A

G
|
G

A

C

S

S

-

E

N

H

G

K

I

Y

G

G

F

L

G

G

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x
C

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T

G
|
G

G
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S

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|
T

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S

A

G

G

L

I

L

L

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A

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G

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D

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T

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H

N

D

N

S

T

M

A

K

K

Y

L

I

I

D

G

V

V

N

E

L

H

N

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K

D

D

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D

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F

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-

G

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P

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C

Y

Y

A

G

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V

S

P

R

N

E

E

E

G

E

T

L

I

E

E

F

A

I

I

K

R

E

T

L

C

A

A

E

E

L

Q

E

E

G

G

I

V

I

L

L

T

D

D

P

P

V

V

Y
|
Y

T
x
E

G

G

K

K

A

S

M

M

Y

Q

G

G

L

L

T

I

Q

A

E

L

I

I

K

K

K

E

G

D

K

Y

F

F

S

K

K

P

Y

G

K

A

N

N

L

V

L

L

F

Y

I

V

H

H

T

L

G

G

I

A

F

P

G

A

I

L

-

S

-

A

-

Y

-

S

-

F

F

F

P

P

Q

T

K

K

S

T

binding n-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-y-lmethyl]-1-amino-cyclopropanecarboxylic acid: S78 (= S75), N79 (= N76), Q80 (≠ H77), A163 (≠ E153), G164 (= G154), C200 (≠ T189), V201 (≠ G190), T202 (≠ S191), G203 (= G192), S204 (≠ G193), T205 (= T194), Y295 (= Y286), E296 (≠ T287)

Query Sequence

>WP_011989078.1 NCBI__GCF_000016505.1:WP_011989078.1
MIKIPERMNMANLPTKIEKMERLSQKLGGPDIYIKRDDQTGTEISGNKIRKLEFSAAEAL
NKGCNTLITCGGIQSNHCRATAAVAVKLGFKCCLVLNGSNDTEVDGNLLLDKLLGAEIYF
VSQKEYENRRMEIMKEIKTNMENKGLKPYIIPEGASNGIGGFGYYKAVQEIMLQEREMKV
HFDGIVIATGSGGTYSGLLLGSRILNYDAKIYGVNVCQNEKYFKDRIYEILHDSMKYIDV
NLNFSKDEINIIDGYVGRGYALSREEELEFIKELAELEGIILDPVYTGKAMYGLTQEIKK
GKFSKYKNLLFIHTGGIFGIFPQKSQFRV

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory