SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011989356.1 NCBI__GCF_000016505.1:WP_011989356.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 8 hits to proteins with known functional sites (download)

Q12640 Chorismate synthase aro-2; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 1.5.1.38; EC 4.2.3.5 from Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) (see 3 papers)
36% identity, 99% coverage: 4:358/360 of query aligns to 3:390/432 of Q12640

query
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Q12640

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S16 (= S17) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 70-fold.
H17 (= H18) active site; mutation to A: Reduces the chorismate synthase activity 20-fold.
H106 (= H105) active site; mutation to A: Reduces the chorismate synthase activity 10-fold.
S127 (= S127) mutation to A: Does not affect the flavin reductase activity but reduces the chorismate synthase activity 6-fold.
D367 (= D335) active site; mutation D->A,N: Does not affect substrate binding but stringly reduces the chorismate synthase activity.

1um0A Crystal structure of chorismate synthase complexed with fmn (see paper)
39% identity, 98% coverage: 5:358/360 of query aligns to 4:347/365 of 1um0A

query
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binding flavin mononucleotide: H104 (= H105), R123 (≠ H125), S125 (= S127), N241 (≠ P246), G242 (≠ A247), L280 (= L290), G281 (= G291), K296 (= K306), T298 (= T308), P299 (= P309), S300 (= S310), I327 (= I338), R330 (= R341)

P56122 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) (see paper)
39% identity, 98% coverage: 5:358/360 of query aligns to 4:347/365 of P56122

query
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P56122

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RSS 123:125 (≠ HFS 125:127) binding FMN
KPTPS 296:300 (= KPTPS 306:310) binding FMN

2o12A Mycobacterium tuberculosis chorismate synthase in complex with fmn
33% identity, 94% coverage: 15:352/360 of query aligns to 8:354/386 of 2o12A

query
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2o12A

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A

G

G

S

M

I

L

R

K

Y

Y

R

-

G

G

F

F

N

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

H

R

F

A

S

S

G
x
A

R

R

I

E

T

T

A

A

P

A

L

R

V

V

F

A

A

A

G

G

A

T

I

V

C

A

K

R

K

A

I

F

L

L

E

R

D

Q

K

A

-

L

G

G

I

V

L

E

I

V

G

L

S

S

H

H

I

V

K

I

S

S

I

I

G

G

A

A

I

-

E

-

D

-

G

S

S

A

C

P

F

Y

D

E

P

G

V

P

S

P

I

P

S

R

K

A

E

E

S

D

I

L

L

P

K

A

L

I

S

D

K

A

S

S

K

P

F

V

P

R

V

A

L

Y

D

D

E

K

V

A

K

A

G

E

K

A

D

D

M

M

E

I

E

A

S

Q

I

I

L

E

K

A

I

A

K

K

E

K

E

D

G

G

D

D

S

T

V

L

G

G

G

G

V

V

I

V

E

E

T

A

V

V

V

A

V

L

N

G

L

L

P

P

P

V

G

G

I

L

G

G

N

S

P

-

F

-

F

F

D

T

S

S

-

G

-

D

-

H

-

R

V

L

E

D

S

S

S

Q

I

L

S

A

H

A

L

A

L

V

F

M

S

G

I

I

P
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

F

I

G

G

E

D

G

G

F

F

N

Q

I

T

A

A

S

R

M

R

R

R

G

G

S

S

E

R

A

A

N

H

D

D

E

E

Y

M

Y

Y

I

P

S

G

E

P

Q

D

S

G

T

V

M

V

K

R

T

S

Y

-

T

T

N

N

N

R

N

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

T

T

N

N

G

G

M

Q

P

P

V

L

I

R

F

V

R

R

T

A

A

A

I
x
M

K
|
K

P

P

T
x
I

P

S

S
x
T

I

V

A

P

K

R

T

A

Q

L

K

A

T

T

V

V

D

D

I

L

K

A

K

T

K

G

Q

D

N

E

T

A

F

-

L

V

K

A

I

I

E

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

I

A

V

V

P

P

R

A

A

A

V

G

P

V

V

V

V

V

E

E

A

T

V

M

A

V

A

A

I

L

binding flavin mononucleotide: H109 (= H105), A132 (≠ G128), Q250 (≠ P246), A251 (= A247), M308 (≠ I305), K309 (= K306), I311 (≠ T308), T313 (≠ S310), R335 (= R333)

2qhfA Mycobacterium tuberculosis chorismate synthase in complex with nca
33% identity, 96% coverage: 9:352/360 of query aligns to 2:360/392 of 2qhfA

query
sites
2qhfA

I

L

K

R

L

W

S

I

I

T

F

A

G

G

E

E

S

S

H

H

G

G

K

R

A

A

I

L

G

V

I

A

V

V

I

V

D

E

G

G

L

M

K

V

S

A

G

G

I

V

E

H

I

V

D

T

I

S

D

A

Y

D

I

I

K

A

N

D

E

Q

M

L

E

A

R

R

R

R

A

R

P

L

G

G

A

Y

S

G

P
x
R

L

G

S

A

T

R

P

M

R

T

K

F

E

E

T

R

D

D

K

A

F

V

D

T

I

V

L

L

S

S

G

G

Y

I

F

R

N

H

G

G

R

S

T

T

T

L

G

G

T

G

P

P

L

I

S

A

A

I

I

E

I

I

L

G

N

N

N

T

N

E

-

W

-

P

-

K

-

W

-

E

-

T

-

V

-

M

-

A

-

A

-

D

-

P

-

V

-

D

-

P

T

A

R

E

S

L

R

A

D

D

Y

V

E

A

K

R

T

N

A

A

G

P

L

L

L

T

-

R

-

P

R

R

P

P

G

G

H

H

A

A

D

D

F

Y

T

A

G

G

S

M

I

L

R

K

Y

Y

R

-

G

G

F

F

N

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

H

R

F

A

S

S

G
x
A

R

R

I

E

T

T

A

A

P

A

L

R

V

V

F

A

A

A

G

G

A

T

I

V

C

A

K

R

K

A

I

F

L

L

E

R

D

Q

K

A

-

L

G

G

I

V

L

E

I

V

G

L

S

S

H

H

I

V

K

I

S

S

I

I

G

G

A

A

I

-

E

-

D

-

G

S

S

A

C

P

F

Y

D

E

P

G

V

P

S

P

I

P

S

R

K

A

E

E

S

D

I

L

L

P

K

A

L

I

S

D

K

A

S

S

K

P

F

V

P

R

V

A

L

Y

D

D

E

K

V

A

K

A

G

E

K

A

D

D

M

M

E

I

E

A

S

Q

I

I

L

E

K

A

I

A

K

K

E

K

E

D

G

G

D

D

S

T

V

L

G

G

G

G

V

V

I

V

E

E

T

A

V

V

V

A

V

L

N

G

L

L

P

P

P

V

G

G

I

L

G

G

N

S

P

-

F

-

F

F

D

T

S

S

-

G

-

D

-

H

-

R

V

L

E

D

S

S

S

Q

I

L

S

A

H

A

L

A

L

V

F

M

S

G

I
|
I

P
x
Q

A

A

V

I

K

K

G

G

V

V

E

E

F

I

G

G

E

D

G

G

F

F

N

Q

I

T

A

A

S

R

M

R

R

R

G

G

S

S

E

R

A

A

N

H

D

D

E

E

Y

M

Y

Y

I

P

S

G

E

P

Q

D

S

G

T

V

M

V

K

R

T

S

Y

-

T

T

N

N

N

R

N

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

T

T

N

N

G

G

M

Q

P

P

V

L

I

R

F

V

R

R

T

A

A

A

I
x
M

K

K

P

P

T
x
I

P

S

S

T

I

V

A

P

K

R

T

A

Q

L

K

A

T

T

V

V

D

D

I

L

K

A

K

T

K

G

Q

D

N

E

T

A

F

-

L

V

K

A

I

I

E

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

I

A

V

V

P

P

R

A

A

A

V

G

P

V

V

V

V

V

E

E

A

T

V

M

A

V

A

A

I

L

binding nicotinamide: R46 (≠ P53), A138 (≠ G128), I255 (= I245), Q256 (≠ P246), M314 (≠ I305), I317 (≠ T308), R341 (= R333)

P9WPY1 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
33% identity, 96% coverage: 9:352/360 of query aligns to 2:360/401 of P9WPY1

query
sites
P9WPY1

I

L

K

R

L

W

S

I

I

T

F

A

G

G

E

E

S

S

H

H

G

G

K

R

A

A

I

L

G

V

I

A

V

V

I

V

D

E

G

G

L

M

K

V

S

A

G

G

I

V

E

H

I

V

D

T

I

S

D

A

Y

D

I

I

K

A

N

D

E

Q

M

L

E

A

R

R

R
|
R

A

R

P

L

G

G

A

Y

S

G

P
x
R

L

G

S

A

T

R

P

M

R

T

K

F

E

E

T

R

D

D

K

A

F

V

D

T

I

V

L

L

S

S

G

G

Y

I

F

R

N

H

G

G

R

S

T

T

T

L

G

G

T

G

P

P

L

I

S

A

A

I

I

E

I

I

L

G

N

N

N

T

N

E

-

W

-

P

-

K

-

W

-

E

-

T

-

V

-

M

-

A

-

A

-

D

-

P

-

V

-

D

-

P

T

A

R

E

S

L

R

A

D

D

Y

V

E

A

K

R

T

N

A

A

G

P

L

L

L

T

-

R

-

P

R

R

P

P

G

G

H

H

A

A

D

D

F

Y

T

A

G

G

S

M

I

L

R

K

Y

Y

R

-

G

G

F

F

N

D

D

D

Y

A

R

R

G

P

G

V

-

L

G

E

H

R

F

A

S

S

G

A

R

R

I

E

T

T

A

A

P

A

L

R

V

V

F

A

A

A

G

G

A

T

I

V

C

A

K

R

K

A

I

F

L

L

E

R

D

Q

K

A

-

L

G

G

I

V

L

E

I

V

G

L

S

S

H

H

I

V

K

I

S

S

I

I

G

G

A

A

I

-

E

-

D

-

G

S

S

A

C

P

F

Y

D

E

P

G

V

P

S

P

I

P

S

R

K

A

E

E

S

D

I

L

L

P

K

A

L

I

S

D

K

A

S

S

K

P

F

V

P

R

V

A

L

Y

D

D

E

K

V

A

K

A

G

E

K

A

D

D

M

M

E

I

E

A

S

Q

I

I

L

E

K

A

I

A

K

K

E

K

E

D

G

G

D

D

S

T

V

L

G

G

G

G

V

V

I

V

E

E

T

A

V

V

V

A

V

L

N

G

L

L

P

P

P

V

G

G

I

L

G

G

N

S

P

-

F

-

F

F

D

T

S

S

-

G

-

D

-

H

-

R

V

L

E

D

S

S

S

Q

I

L

S

A

H

A

L

A

L

V

F

M

S

G

I

I

P
x
Q

A
|
A

V

I

K

K

G

G

V

V

E

E

F

I

G

G

E

D

G

G

F

F

N

Q

I

T

A

A

S

R

M

R

R

R

G

G

S

S

E

R

A

A

N

H

D

D

E

E

Y

M

Y

Y

I

P

S

G

E

P

Q

D

S

G

T

V

M

V

K

R

T

S

Y

-

T

T

N

N

N

R

N

A

G

G

G

G

V

L

L

E

G

G

G

G

I

M

T

T

N

N

G

G

M

Q

P

P

V

L

I

R

F

V

R

R

T

A

A

A

I

M

K
|
K

P
|
P

T
x
I

P
x
S

S
x
T

I

V

A

P

K

R

T

A

Q

L

K

A

T

T

V

V

D

D

I

L

K

A

K

T

K

G

Q

D

N

E

T

A

F

-

L

V

K

A

I

I

E

H

G

Q

R
|
R

H

S

D

D

P

V

C

C

I

A

V

V

P

P

R

A

A

A

V

G

P

V

V

V

V

V

E

E

A

T

V

M

A

V

A

A

I

L

R40 (= R47) binding NADP(+)
R46 (≠ P53) binding NADP(+)
QA 256:257 (≠ PA 246:247) binding FMN
KPIST 315:319 (≠ KPTPS 306:310) binding FMN
R341 (= R333) binding FMN

1qxoA Crystal structure of chorismate synthase complexed with oxidized fmn and epsp (see paper)
32% identity, 94% coverage: 15:351/360 of query aligns to 7:355/388 of 1qxoA

query
sites
1qxoA

G

G

E

E

S

S

H

H

G

G

K

P

A

R

I

L

G

T

I

A

V

I

I

I

D

E

G

G

L

I

K

P

S

A

G

G

I

L

E

P

I

L

D

T

I

A

D

E

Y

D

I

I

K

N

N

E

E

D

M

L

E

R

R

R

R
|
R

A

Q

P

G

G

G

A

Y

S

G

P
x
R

L

G

S

G

T
x
R

P
x
M

R

K

K

I

E

E

T

N

D

D

K

Q

F

V

D

V

I

F

L

T

S

S

G

G

Y

V

F

R

N

H

G

G

R

K

T

T

T

T

G

G

T

A

P

P

L

I

S

T

A

M

I

D

I

V

L

I

N

N

N

-

N

-

T

-

R

-

S

-

R

K

D

D

Y

H

E

Q

K

K

T

W

A

L

G

D

L

I

L

M

-

S

-

A

-

E

-

D

-

I

-

E

-

D

-

R

-

L

-

K

-

S

-

K

-

R

-

K

-

I

-

T

-

H

-

P

R

R

P

P

G

G

H
|
H

A

A

D

D

F

L

T

V

G

G

S

G

I

I

R

K

Y

Y

R

R

G

-

F

F

N

D

D

D

Y

L

R

R

G

N

G

S

-

L

G

E

H

R

F

S

S

S

G
x
A

R
|
R

I

E

T

T

A

T

P

M

L

R

V

V

F

A

A

V

G

G

A

A

I

V

C

A

K

K

K

R

I

L

L

L

E

A

D

E

K

L

G

D

I

M

L

E

I

I

G

A

S

N

H

H

I

V

K

V

S

V

I

F

G

G

A

-

I

-

E

-

D

-

G

G

S

K

C

E

F

I

D

D

-

V

P

P

V

E

S

N

I

L

S

T

K

V

E

A

S

E

I

I

-

K

L

Q

K

R

L

A

S

A

K

Q

S

S

K

E

F

V

P

S

V

I

L

V

D

N

E

Q

V

E

K

R

G

E

K

Q

D

E

M

I

E

K

E

D

S

Y

I

I

L

D

K

Q

I

I

K

K

E

R

E

D

G

G

D

D

S

T

V

I

G

G

G

G

V

V

I

V

E

E

T

T

V

V

V

V

V

G

N

G

L

V

P

P

P

V

G

G

I

L

G

G

N

S

-

Y

-

V

P

Q

F

W

F

D

D

R

S

K

V

L

E

D

S

A

S

R

I

L

S

A

H

Q

L

A

L

V

F

V

S

S

I

I

P
x
N

A
|
A

V

F

K

K

G

G

V

V

E

E

F

F

G

G

E

L

G

G

F

F

N

E

I

A

A

G

S

Y

M

R

R

K

G

G

S

S

E

Q

A

V

N

M

D

D

E

E

Y

I

Y

L

I

W

S

S

E

K

Q

E

S

D

T

G

M

Y

K

T

T

R

Y

R

T

T

N

N

N

N

N

L

G

G

G

G

V

F

L

E

G

G

G

G

I

M

T

T

N

N

G

G

M

Q

P

P

V

I

I

V

F

V

R

R

T

G

A

V

I
x
M

K
|
K

P

P

T
x
I

P
|
P

S
x
T

I

L

A

Y

K

K

T

P

Q

L

K

M

T

S

V

V

D

D

I

I

K

E

K

T

K

H

Q

E

N

P

T

Y

F

K

L

A

K

T

I

V

E

E

G

-

R

R

H

S

D

D

P

P

C

T

I

A

V

L

P

P

R

A

A

A

V

G

P

M

V

V

V

M

E

E

A

A

V

V

A

V

A

A

binding 5-[(1-carboxyvinyl)oxy]-4-hydroxy-3-(phosphonooxy)cyclohex-1-ene-1-carboxylic acid: R39 (= R47), R45 (≠ P53), R48 (≠ T56), M49 (≠ P57), A133 (≠ G128), R134 (= R129)
binding flavin mononucleotide: H110 (= H105), A133 (≠ G128), N251 (≠ P246), A252 (= A247), M310 (≠ I305), K311 (= K306), I313 (≠ T308), P314 (= P309), T315 (≠ S310)

P0A2Y6 Chorismate synthase; CS; 5-enolpyruvylshikimate-3-phosphate phospholyase; EC 4.2.3.5 from Streptococcus pneumoniae serotype 4 (strain ATCC BAA-334 / TIGR4) (see paper)
32% identity, 94% coverage: 15:351/360 of query aligns to 7:355/388 of P0A2Y6

query
sites
P0A2Y6

G

G

E

E

S

S

H

H

G

G

K

P

A

R

I

L

G

T

I

A

V

I

I

I

D

E

G

G

L

I

K

P

S

A

G

G

I

L

E

P

I

L

D

T

I

A

D

E

Y

D

I

I

K

N

N

E

E

D

M

L

E

R

R

R

R

R

A

Q

P

G

G

G

A

Y

S

G

P

R

L

G

S

G

T

R

P

M

R

K

K

I

E

E

T

N

D

D

K

Q

F

V

D

V

I

F

L

T

S

S

G

G

Y

V

F

R

N

H

G

G

R

K

T

T

T

T

G

G

T

A

P

P

L

I

S

T

A

M

I

D

I

V

L

I

N

N

N

-

N

-

T

-

R

-

S

-

R

K

D

D

Y

H

E

Q

K

K

T

W

A

L

G

D

L

I

L

M

-

S

-

A

-

E

-

D

-

I

-

E

-

D

-

R

-

L

-

K

-

S

-

K

-

R

-

K

-

I

-

T

-

H

-

P

R

R

P

P

G

G

H

H

A

A

D

D

F

L

T

V

G

G

S

G

I

I

R

K

Y

Y

R

R

G

-

F

F

N

D

D

D

Y

L

R

R

G

N

G

S

-

L

G

E

H

R

F

S

S

S

G

A

R

R

I

E

T

T

A

T

P

M

L

R

V

V

F

A

A

V

G

G

A

A

I

V

C

A

K

K

K

R

I

L

L

L

E

A

D

E

K

L

G

D

I

M

L

E

I

I

G

A

S

N

H

H

I

V

K

V

S

V

I

F

G

G

A

-

I

-

E

-

D

-

G

G

S

K

C

E

F

I

D

D

-

V

P

P

V

E

S

N

I

L

S

T

K

V

E

A

S

E

I

I

-

K

L

Q

K

R

L

A

S

A

K

Q

S

S

K

E

F

V

P

S

V

I

L

V

D

N

E

Q

V

E

K

R

G

E

K

Q

D

E

M

I

E

K

E

D

S

Y

I

I

L

D

K

Q

I

I

K

K

E

R

E

D

G

G

D

D

S

T

V

I

G

G

G

G

V

V

I

V

E

E

T

T

V

V

V

V

V

G

N

G

L

V

P

P

P

V

G

G

I

L

G

G

N

S

-

Y

-

V

P

Q

F

W

F

D

D

R

S

K

V

L

E

D

S

A

S

R

I

L

S

A

H

Q

L

A

L

V

F

V

S

S

I

I

P
x
N

A
|
A

V

F

K

K

G

G

V

V

E

E

F

F

G

G

E

L

G

G

F

F

N

E

I

A

A

G

S

Y

M

R

R

K

G

G

S

S

E

Q

A

V

N

M

D

D

E

E

Y

I

Y

L

I

W

S

S

E

K

Q

E

S

D

T

G

M

Y

K

T

T

R

Y

R

T

T

N

N

N

N

N

L

G

G

G

G

V

F

L

E

G
|
G

G

G

I

M

T

T

N

N

G

G

M

Q

P

P

V

I

I

V

F

V

R

R

T

G

A

V

I

M

K
|
K

P
|
P

T
x
I

P
|
P

S
x
T

I

L

A

Y

K

K

T

P

Q

L

K

M

T

S

V

V

D

D

I

I

K

E

K

T

K

H

Q

E

N

P

T

Y

F

K

L

A

K

T

I

V

E

E

G

-

R

R

H

S

D

D

P

P

C

T

I

A

V

L

P

P

R

A

A

A

V

G

P

M

V

V

V

M

E

E

A

A

V

V

A

V

A

A

NA 251:252 (≠ PA 246:247) binding FMN
G296 (= G291) binding FMN
KPIPT 311:315 (≠ KPTPS 306:310) binding FMN

Query Sequence

>WP_011989356.1 NCBI__GCF_000016505.1:WP_011989356.1
MSGVWGNKIKLSIFGESHGKAIGIVIDGLKSGIEIDIDYIKNEMERRAPGASPLSTPRKE
TDKFDILSGYFNGRTTGTPLSAIILNNNTRSRDYEKTAGLLRPGHADFTGSIRYRGFNDY
RGGGHFSGRITAPLVFAGAICKKILEDKGILIGSHIKSIGAIEDGSCFDPVSISKESILK
LSKSKFPVLDEVKGKDMEESILKIKEEGDSVGGVIETVVVNLPPGIGNPFFDSVESSISH
LLFSIPAVKGVEFGEGFNIASMRGSEANDEYYISEQSTMKTYTNNNGGVLGGITNGMPVI
FRTAIKPTPSIAKTQKTVDIKKKQNTFLKIEGRHDPCIVPRAVPVVEAVAAIGILNLMEY

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory