SitesBLAST
Comparing WP_011989421.1 NCBI__GCF_000016505.1:WP_011989421.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
44% identity, 76% coverage: 2:313/413 of query aligns to 5:306/402 of 4pg7A
Sites not aligning to the query:
Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
39% identity, 69% coverage: 64:349/413 of query aligns to 67:361/435 of Q5F8J4
Sites not aligning to the query:
- 45 L→R: Shows a marked increase in the catalytic efficiency with NADP(+).
- 45:46 LS→RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; LS→RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
36% identity, 87% coverage: 2:361/413 of query aligns to 5:372/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G8), L12 (≠ Y9), G13 (= G10), N14 (≠ V11), V15 (= V12), V45 (= V42), R46 (= R43), R47 (≠ N44), R52 (≠ D50), I63 (= I61), L78 (≠ A75), M79 (= M76), P84 (= P81), A102 (= A99), K104 (= K101), G306 (= G298), T310 (= T302)
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
35% identity, 75% coverage: 2:310/413 of query aligns to 4:302/332 of 6a0tB
- active site: D191 (= D197), K195 (= K201)
- binding l-homoserine: N150 (= N155), G151 (= G156), T152 (= T157), Y178 (≠ F184), E180 (= E186), D186 (= D192), K195 (= K201)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G8), G11 (≠ Y9), G12 (= G10), T13 (≠ V11), V14 (= V12), L42 (= L41), V43 (= V42), R44 (= R43), D45 (≠ N44), K48 (= K47), R50 (≠ I49), A73 (= A75), M74 (= M76), G75 (= G77), A97 (= A99), N98 (= N100), G177 (= G183), E180 (= E186), A289 (= A297), G290 (= G298), T294 (= T302)
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
35% identity, 75% coverage: 2:310/413 of query aligns to 4:302/331 of 6a0sA
- active site: D191 (= D197), K195 (= K201)
- binding l-homoserine: K99 (= K101), N150 (= N155), G151 (= G156), T152 (= T157), Y178 (≠ F184), E180 (= E186), D186 (= D192), K195 (= K201)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ Y9), G12 (= G10), T13 (≠ V11), V14 (= V12), L42 (= L41), V43 (= V42), R44 (= R43), D45 (≠ N44), K48 (= K47), R50 (≠ I49), A73 (= A75), M74 (= M76), A97 (= A99), K99 (= K101), G177 (= G183), E180 (= E186), A289 (= A297), G290 (= G298), T294 (= T302)
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
35% identity, 75% coverage: 2:310/413 of query aligns to 4:302/331 of 2ejwA
F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
33% identity, 76% coverage: 4:318/413 of query aligns to 3:301/304 of F9VNG5
- Y8 (= Y9) binding NADP(+)
- N10 (≠ V11) binding NADP(+)
- V11 (= V12) binding NADP(+)
- R38 (= R43) binding NADP(+)
- R39 (≠ K47) binding NADP(+)
- S73 (≠ M76) binding NADP(+)
- T100 (≠ A99) binding NADP(+)
- K102 (= K101) binding NADP(+)
- G182 (= G183) binding NADP(+)
- E185 (= E186) binding NADP(+)
- G284 (= G298) binding NADP(+)
Sites not aligning to the query:
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
33% identity, 76% coverage: 4:318/413 of query aligns to 3:297/300 of 7f4cA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ L7), G7 (= G8), Y8 (= Y9), G9 (= G10), N10 (≠ V11), V11 (= V12), T37 (≠ V42), R38 (= R43), R39 (≠ K47), V72 (≠ A75), S73 (≠ M76), S74 (≠ G77), T100 (≠ A99), K102 (= K101), G127 (≠ A126), S131 (≠ G130), E185 (= E186), G280 (= G298), A284 (= A305)
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
33% identity, 76% coverage: 4:318/413 of query aligns to 3:299/302 of 5x9dA
- active site: D196 (= D197), K200 (= K201)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ L7), G7 (= G8), Y8 (= Y9), G9 (= G10), N10 (≠ V11), V11 (= V12), T37 (≠ V42), R38 (= R43), V72 (≠ A75), S73 (≠ M76), S74 (≠ G77), P82 (= P81), T100 (≠ A99), N101 (= N100), K102 (= K101), G127 (≠ A126), S131 (≠ G130), N155 (= N155), G156 (= G156), T157 (= T157), Y183 (≠ F184), A184 (= A185), E185 (= E186), D191 (= D192), D196 (= D197), K200 (= K201), A281 (= A297), G282 (= G298), A286 (= A305)
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
31% identity, 75% coverage: 2:310/413 of query aligns to 3:308/319 of 4xb2A
- active site: D211 (= D197), K215 (= K201)
- binding l-homoserine: A171 (≠ G156), S172 (≠ T157), D206 (= D192), K215 (= K201)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L7), F10 (≠ Y9), G11 (= G10), T12 (≠ V11), V13 (= V12), R40 (vs. gap), V91 (≠ M76), S92 (≠ G77), S93 (≠ G78), S114 (≠ A99), N115 (= N100), K116 (= K101), S141 (≠ A126), G295 (≠ A297), T300 (= T302)
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
31% identity, 75% coverage: 2:310/413 of query aligns to 3:308/319 of 4xb1A
- active site: D211 (= D197), K215 (= K201)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ L7), F10 (≠ Y9), G11 (= G10), T12 (≠ V11), V13 (= V12), D39 (vs. gap), R40 (vs. gap), K57 (≠ V42), V91 (≠ M76), S92 (≠ G77), S93 (≠ G78), S114 (≠ A99), K116 (= K101), S141 (≠ A126), G295 (≠ A297), T300 (= T302)
O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
31% identity, 75% coverage: 2:310/413 of query aligns to 3:308/319 of O58802
- F10 (≠ Y9) binding NADPH
- T12 (≠ V11) binding NADPH
- V13 (= V12) binding NADPH
- R40 (vs. gap) binding NADPH; mutation to A: Increases activity with NADP.
- K57 (≠ V42) binding NADPH; mutation to A: Increases activity with NADP.
- S92 (≠ G77) binding NADPH
- S93 (≠ G78) binding NADPH
- S114 (≠ A99) binding NADPH
- K116 (= K101) binding NADPH
- E140 (= E125) binding Na(+)
- V143 (= V128) binding Na(+)
- A145 (≠ G130) binding Na(+)
- T147 (≠ I132) binding Na(+)
- G296 (= G298) binding NADPH
3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
27% identity, 75% coverage: 2:310/413 of query aligns to 3:313/319 of 3ingA
- active site: D209 (= D197), K213 (= K201)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G8), T10 (≠ Y9), G11 (= G10), N12 (≠ V11), V13 (= V12), D38 (≠ E33), S39 (≠ E34), K57 (= K52), C85 (≠ A75), T86 (≠ M76), P87 (vs. gap), A112 (= A99), N113 (= N100), K114 (= K101), A139 (= A126), E198 (= E186), S199 (≠ A187), P302 (≠ M299), T305 (= T302)
3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
28% identity, 54% coverage: 2:226/413 of query aligns to 4:241/321 of 3jsaA