SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_011996129.1 NCBI__GCF_000017645.1:WP_011996129.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WGB5 O-succinylhomoserine sulfhydrylase; OSH sulfhydrylase; OSHS sulfhydrylase; EC 2.5.1.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
56% identity, 93% coverage: 26:402/406 of query aligns to 24:404/406 of P9WGB5

query
sites
P9WGB5

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K219 (= K221) modified: N6-(pyridoxal phosphate)lysine

6egrA Crystal structure of citrobacter freundii methionine gamma-lyase with v358y replacement (see paper)
46% identity, 94% coverage: 22:402/406 of query aligns to 9:391/396 of 6egrA

query
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6egrA

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binding pyridoxal-5'-phosphate: G87 (= G99), I88 (≠ M100), Y112 (≠ F124), E155 (= E167), D184 (= D196), T186 (≠ V198), F187 (= F199), S206 (= S218), T208 (= T220), K209 (= K221)

5m3zA Crystal structure of citrobacter freundii methionine gamma-lyase with c115h replacement in the complex with l-norleucine (see paper)
46% identity, 94% coverage: 22:402/406 of query aligns to 8:390/395 of 5m3zA

query
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5m3zA

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active site: R58 (= R71), Y111 (≠ F124), D183 (= D196), K208 (= K221)
binding norleucine: Y111 (≠ F124), H113 (≠ S126), K208 (= K221), V336 (≠ N348), S337 (≠ N349)
binding pyridoxal-5'-phosphate: G86 (= G99), I87 (≠ M100), Y111 (≠ F124), E154 (= E167), D183 (= D196), T185 (≠ V198), S205 (= S218), T207 (= T220), K208 (= K221)
binding 2-[o-phosphonopyridoxyl]-amino-hexanoic acid: G86 (= G99), I87 (≠ M100), Y111 (≠ F124), D183 (= D196), S205 (= S218), T207 (= T220), K208 (= K221), V336 (≠ N348), S337 (≠ N349), R372 (= R384)

4omaA The crystal structure of methionine gamma-lyase from citrobacter freundii in complex with l-cycloserine pyridoxal-5'-phosphate (see paper)
46% identity, 94% coverage: 22:402/406 of query aligns to 9:391/396 of 4omaA

query
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4omaA

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P

R

K

F

F

G

G

I

I

E

N

T

V

T

S

L

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

W

I

Q

R

A

A

A

M

R

R

P

P

S

E

T

T

K

K

V

V

F

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

L

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

Q

G

I

I

A

A

H

H

A

Q

G

G

A

A

R

L

L

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

L

C

Q

Q

H

Q

P

P

F

L

E

Q

L

L

G

G

A

A

D

D

I

I

V

V

Y

H

S
|
S

T

V

T
|
T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

S

K

Q

Q

Q

E

F

F

V

I

T

D

D

Q

H

A

L

R

Q

F

T

V

F

G

L

L

R

K

Q

D

-

I

T

T

G

G

P

G

S

C

I

M

S

S

P

P

F

F

N

N

A

A

W

W

I

L

M

T

L

L

K

R

G

G

L

V

E

K

T

T

L

L

A

G

L

I

R

R

V

M

E

E

R

R

M

H

Q

C

A

E

S

N

A

A

G

L

R

K

I

I

A

A

D

R

A

F

L

L

A

E

G

G

L

H

P

P

K

S

I

I

E

T

T

R

V

V

L

Y

Y

Y

P

P

G

G

R

L

A

S

D

S

H

H

P

P

Q

Q

A

Y

E

E

L

L

A

G

R

Q

R

R

Q

Q

M

M

K

S

G

L

G

P

G

G

T

G

L

I

V

I

T

S

F

F

V

E

V

I

E

A

G

G

K

L

E

E

G

A

A

G

F

R

R

R

F

M

A

I

N

N

A

S

L

V

G

E

L

L

I

C

R

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

A

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

P

F

V

T

A

P

P

E

E

A

E

R

R

A

L

E

K

M

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

P

D

E

D

D

L

I

I

I

A

N

D

D

L

L

A

E

T

H

A

A

active site: R59 (= R71), Y112 (≠ F124), D184 (= D196), K209 (= K221)
binding [5-hydroxy-6-methyl-4-({[(4E)-3-oxo-1,2-oxazolidin-4-ylidene]amino}methyl)pyridin-3-yl]methyl dihydrogen phosphate: G87 (= G99), I88 (≠ M100), Y112 (≠ F124), D184 (= D196), S206 (= S218), T208 (= T220), K209 (= K221), V337 (≠ N348), S338 (≠ N349), R373 (= R384)

3jwbA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with norleucine (see paper)
46% identity, 94% coverage: 22:402/406 of query aligns to 9:391/396 of 3jwbA

query
sites
3jwbA

E

N

T

T

R

Q

L

I

V

V

H

H

G

A

G

G

T

Q

R

P

S

D

P

P

Y

S

-

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

N

S

A

A

E

E

S

Q

C

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

D

E

P

S

G

G

F

Y

V

I

Y

Y

S

T

R
|
R

Y

L

G

G

N

N

P

P

T

T

A

D

M

A

F

L

E

E

E

K

R

K

M

L

A

A

L

V

L

L

E

E

G

R

A

G

E

E

A

A

A

G

R

L

A

A

T

T

A

A

S

S

G

G

M

I

A

S

A

A

V

I

N

T

A

T

L

L

C

T

Q

L

L

C

S

Q

A

Q

G

G

D

D

H

H

V

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

I

F

L

L

E

S

D

H

F

S

L

M

P

P

R

K

F

F

G

G

I

I

E

N

T

V

T

S

L

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

W

I

Q

R

A

A

A

M

R

R

P

P

S

E

T

T

K

K

V

V

F

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

L

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

Q

G

I

I

A

A

H

H

A

Q

G

G

A

A

R

L

L

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

L

C

Q

Q

H

Q

P

P

F

L

E

Q

L

L

G

G

A

A

D

D

I

I

V

V

Y

H

S

S

T

V

T

T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

S

K

Q

Q

Q

E

F

F

V

I

T

D

D

Q

H

A

L

R

Q

F

T

V

F

G

L

L

R

K

Q

D

-

I

T

T

G

G

P

G

S

C

I

M

S

S

P

P

F

F

N

N

A

A

W

W

I

L

M

T

L

L

K

R

G

G

L

V

E

K

T

T

L

L

A

G

L

I

R

R

V

M

E

E

R

R

M

H

Q

C

A

E

S

N

A

A

G

L

R

K

I

I

A

A

D

R

A

F

L

L

A

E

G

G

L

H

P

P

K

S

I

I

E

T

T

R

V

V

L

Y

Y

Y

P

P

G

G

R

L

A

S

D

S

H

H

P

P

Q

Q

A

Y

E

E

L

L

A

G

R

Q

R

R

Q

Q

M

M

K

S

G

L

G

P

G

G

T

G

L

I

V

I

T

S

F

F

V

E

V

I

E

A

G

G

K

L

E

E

G

A

A

G

F

R

R

R

F

M

A

I

N

N

A

S

L

V

G

E

L

L

I

C

R

L

I

L

S

A

N

V

N
x
S

L

L

G

G

D

D

A

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

P

F

V

T

A

P

P

E

E

A

E

R

R

A

L

E

K

M

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

P

D

E

D

D

L

I

I

I

A

N

D

D

L

L

A

E

T

H

A

A

active site: R59 (= R71), Y112 (≠ F124), D184 (= D196), K209 (= K221)
binding norleucine: Y112 (≠ F124), K209 (= K221), S338 (≠ N349), R373 (= R384)

3jwaA Crystal structure of l-methionine gamma-lyase from citrobacter freundii with methionine phosphinate (see paper)
46% identity, 94% coverage: 22:402/406 of query aligns to 9:391/396 of 3jwaA

query
sites
3jwaA

E

N

T

T

R

Q

L

I

V

V

H

H

G

A

G

G

T

Q

R

P

S

D

P

P

Y

S

-

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

N

S

A

A

E

E

S

Q

C

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

D

E

P

S

G

G

F

Y

V

I

Y

Y

S

T

R
|
R

Y

L

G

G

N

N

P

P

T

T

A

D

M

A

F

L

E

E

E

K

R

K

M

L

A

A

L

V

L

L

E

E

G

R

A

G

E

E

A

A

A

G

R

L

A

A

T

T

A

A

S

S

G

G

M

I

A

S

A

A

V

I

N

T

A

T

L

L

C

T

Q

L

L

C

S

Q

A

Q

G

G

D

D

H

H

V

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

I

F

L

L

E

S

D

H

F

S

L

M

P

P

R

K

F

F

G

G

I

I

E

N

T

V

T

S

L

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

W

I

Q

R

A

A

A

M

R

R

P

P

S

E

T

T

K

K

V

V

F

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

L

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

Q

G

I

I

A

A

H

H

A

Q

G

G

A

A

R

L

L

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

L

C

Q

Q

H

Q

P

P

F

L

E

Q

L

L

G

G

A

A

D

D

I

I

V

V

Y

H

S

S

T

V

T

T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

S

K

Q

Q

Q

E

F

F

V

I

T

D

D

Q

H

A

L

R

Q

F

T

V

F

G

L

L

R

K

Q

D

-

I

T

T

G

G

P

G

S

C

I

M

S

S

P

P

F

F

N

N

A

A

W

W

I

L

M

T

L

L

K

R

G

G

L

V

E

K

T

T

L

L

A

G

L

I

R

R

V

M

E

E

R

R

M

H

Q

C

A

E

S

N

A

A

G

L

R

K

I

I

A

A

D

R

A

F

L

L

A

E

G

G

L

H

P

P

K

S

I

I

E

T

T

R

V

V

L

Y

Y

Y

P

P

G

G

R

L

A

S

D

S

H

H

P

P

Q

Q

A

Y

E

E

L

L

A

G

R

Q

R

R

Q

Q

M

M

K

S

G

L

G

P

G

G

T

G

L

I

V

I

T

S

F

F

V

E

V

I

E

A

G

G

K

L

E

E

G

A

A

G

F

R

R

R

F

M

A

I

N

N

A

S

L

V

G

E

L

L

I

C

R

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

A

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

P

F

V

T

A

P

P

E

E

A

E

R

R

A

L

E

K

M

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

P

D

E

D

D

L

I

I

I

A

N

D

D

L

L

A

E

T

H

A

A

active site: R59 (= R71), Y112 (≠ F124), D184 (= D196), K209 (= K221)
binding (1-amino-3-methylsulfanyl-propyl)-phosphinic acid: Y112 (≠ F124), K209 (= K221), V337 (≠ N348), S338 (≠ N349), R373 (= R384)

3jw9A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with s-ethyl-cysteine (see paper)
46% identity, 94% coverage: 22:402/406 of query aligns to 9:391/396 of 3jw9A

query
sites
3jw9A

E

N

T

T

R

Q

L

I

V

V

H

H

G

A

G

G

T

Q

R

P

S

D

P

P

Y

S

-

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

N

S

A

A

E

E

S

Q

C

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

D

E

P

S

G

G

F

Y

V

I

Y

Y

S

T

R
|
R

Y

L

G

G

N

N

P

P

T

T

A

D

M

A

F

L

E

E

E

K

R

K

M

L

A

A

L

V

L

L

E

E

G

R

A

G

E

E

A

A

A

G

R

L

A

A

T

T

A

A

S

S

G

G

M

I

A

S

A

A

V

I

N

T

A

T

L

L

C

T

Q

L

L

C

S

Q

A

Q

G

G

D

D

H

H

V

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

I

F

L

L

E

S

D

H

F

S

L

M

P

P

R

K

F

F

G

G

I

I

E

N

T

V

T

S

L

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

W

I

Q

R

A

A

A

M

R

R

P

P

S

E

T

T

K

K

V

V

F

V

F

Y

L

I

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

L

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

Q

G

I

I

A

A

H

H

A

Q

G

G

A

A

R

L

L

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

L

C

Q

Q

H

Q

P

P

F

L

E

Q

L

L

G

G

A

A

D

D

I

I

V

V

Y

H

S

S

T

V

T

T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

S

K

Q

Q

Q

E

F

F

V

I

T

D

D

Q

H

A

L

R

Q

F

T

V

F

G

L

L

R

K

Q

D

-

I

T

T

G

G

P

G

S

C

I

M

S

S

P

P

F

F

N

N

A

A

W

W

I

L

M

T

L

L

K

R

G

G

L

V

E

K

T

T

L

L

A

G

L

I

R

R

V

M

E

E

R

R

M

H

Q

C

A

E

S

N

A

A

G

L

R

K

I

I

A

A

D

R

A

F

L

L

A

E

G

G

L

H

P

P

K

S

I

I

E

T

T

R

V

V

L

Y

Y

Y

P

P

G

G

R

L

A

S

D

S

H

H

P

P

Q

Q

A

Y

E

E

L

L

A

G

R

Q

R

R

Q

Q

M

M

K

S

G

L

G

P

G

G

T

G

L

I

V

I

T

S

F

F

V

E

V

I

E

A

G

G

K

L

E

E

G

A

A

G

F

R

R

R

F

M

A

I

N

N

A

S

L

V

G

E

L

L

I

C

R

L

I

L

S

A

N
x
V

N
x
S

L

L

G

G

D

D

A

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

P

F

V

T

A

P

P

E

E

A

E

R

R

A

L

E

K

M

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

P

D

E

D

D

L

I

I

I

A

N

D

D

L

L

A

E

T

H

A

A

active site: R59 (= R71), Y112 (≠ F124), D184 (= D196), K209 (= K221)
binding S-ethyl-L-cysteine: Y112 (≠ F124), K209 (= K221), V337 (≠ N348), S338 (≠ N349)

4hf8A Crystal structure of l-methionine gamma-lyase from citrobacter freundii with glycine (see paper)
46% identity, 94% coverage: 22:402/406 of query aligns to 9:391/396 of 4hf8A

query
sites
4hf8A

E

N

T

T

R

Q

L

I

V

V

H

H

G

A

G

G

T

Q

R

P

S

D

P

P

Y

S

-

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

N

S

A

A

E

E

S

Q

C

G

E

A

A

A

R

R

F

F

K

A

G

L

E

E

D

E

P

S

G

G

F

Y

V

I

Y

Y

S

T

R
|
R

Y

L

G

G

N

N

P

P

T

T

A

D

M

A

F

L

E

E

E

K

R

K

M

L

A

A

L

V

L

L

E

E

G

R

A

G

E

E

A

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

N

T

A

T

L

L

C

T

Q

L

L

C

S

Q

A

Q

G

G

D

D

H

H

V

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

I

F

L

L

E

S

D

H

F

S

L

M

P

P

R

K

F

F

G

G

I

I

E

N

T

V

T

R

L

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

W

I

Q

R

A

A

A

M

R

R

P

P

S

E

T

T

K

K

V

V

F

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N
|
N

P

P

T

T

L

L

E

S

L

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

Q

G

I

I

A

A

H

H

A

Q

G

G

A

A

R

L

L

L

V

V

D
|
D

N

N

V

T

F

F

A

M

T

S

P

P

L

Y

L

C

Q

Q

H

Q

P

P

F

L

E

Q

L

L

G

G

A

A

D

D

I

I

V

V

Y

H

S
|
S

T

V

T

T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

S

K

Q

Q

Q

E

F

F

V

I

T

D

D

Q

H

A

L

R

Q

F

T

V

F

G

L

L

R

K

Q

D

-

I

T

T

G

G

P

G

S

C

I

M

S

S

P

P

F

F

N

N

A

A

W

W

I

L

M

T

L

L

K

R

G

G

L

V

E

K

T

T

L

L

A

G

L

I

R

R

V

M

E

E

R

R

M

H

Q

C

A

E

S

N

A

A

G

L

R

K

I

I

A

A

D

R

A

F

L

L

A

E

G

G

L

H

P

P

K

S

I

I

E

T

T

R

V

V

L

Y

Y

Y

P

P

G

G

R

L

A

S

D

S

H

H

P

P

Q

Q

A

Y

E

E

L

L

A

G

R

Q

R

R

Q

Q

M

M

K

S

G

L

G

P

G

G

T

G

L

I

V

I

T

S

F

F

V

E

V

I

E

A

G

G

K

L

E

E

G

A

A

G

F

R

R

R

F

M

A

I

N

N

A

S

L

V

G

E

L

L

I

C

R

L

I

L

S

A

N

V

N
x
S

L

L

G

G

D

D

A

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

P

F

V

T

A

P

P

E

E

A

E

R

R

A

L

E

K

M

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

P

D

E

D

D

L

I

I

I

A

N

D

D

L

L

A

E

T

H

A

A

active site: R59 (= R71), Y112 (≠ F124), D184 (= D196), K209 (= K221)
binding n-glycine-[3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-yl-methane]: G87 (= G99), I88 (≠ M100), Y112 (≠ F124), E155 (= E167), N159 (= N171), D184 (= D196), S206 (= S218), K209 (= K221), S338 (≠ N349), R373 (= R384)

3mkjA Methionine gamma-lyase from citrobacter freundii with pyridoximine-5'- phosphate (see paper)
45% identity, 94% coverage: 22:402/406 of query aligns to 9:380/386 of 3mkjA

query
sites
3mkjA

E

N

T

T

R

Q

L

I

V

V

H

H

G

A

G

G

T

Q

R

P

S

D

P

P

Y

S

-

T

G

G

E

A

T

L

S

S

E

T

A

P

L

I

F

F

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

N

S

A

A

E

E

S

Q

C

G

E

A

A

A

R

R

F

F

K

-

G

-

E

-

D

-

P

-

G

-

F

-

V

-

Y

-

S

-

R

-

Y

L

G

G

N

N

P

P

T

T

A

D

M

A

F

L

E

E

E

K

R

K

M

L

A

A

L

V

L

L

E

E

G

R

A

G

E

E

A

A

A

G

R

L

A

A

T

T

A

A

S

S

G
|
G

M
x
I

A

S

A

A

V

I

N

T

A

T

L

L

C

T

Q

L

L

C

S

Q

A

Q

G

G

D

D

H

H

V

I

V

V

A

S

A

A

R

S

A

A

L

I

F
x
Y

G

G

S

C

C

T

R

H

W

A

I

F

L

L

E

S

D

H

F

S

L

M

P

P

R

K

F

F

G

G

I

I

E

N

T

V

T

S

L

F

V

V

D

D

G

A

T

A

D

K

L

P

A

E

W

I

Q

R

A

A

A

M

R

R

P

P

S

E

T

T

K

K

V

V

F

V

F

Y

L

I

E
|
E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

S

L

L

I

V

D

D

I

I

A

E

A

T

V

V

A

A

Q

G

I

I

A

A

H

H

A

Q

G

G

A

A

R

L

L

L

V

V

D
|
D

N

N

V

T

F
|
F

A

M

T

S

P

P

L

Y

L

C

Q

Q

H

Q

P

P

F

L

E

Q

L

L

G

G

A

A

D

D

I

I

V

V

Y

H

S
|
S

T

V

T
|
T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

G

V

I

I

I

L

V

S

G

S

K

Q

Q

Q

E

F

F

V

I

T

D

D

Q

H

A

L

R

Q

F

T

V

F

G

L

L

R

K

Q

D

-

I

T

T

G

G

P

G

S

C

I

M

S

S

P

P

F

F

N

N

A

A

W

W

I

L

M

T

L

L

K

R

G

G

L

V

E

K

T

T

L

L

A

G

L

I

R

R

V

M

E

E

R

R

M

H

Q

C

A

E

S

N

A

A

G

L

R

K

I

I

A

A

D

R

A

F

L

L

A

E

G

G

L

H

P

P

K

S

I

I

E

T

T

R

V

V

L

Y

Y

Y

P

P

G

G

R

L

A

S

D

S

H

H

P

P

Q

Q

A

Y

E

E

L

L

A

G

R

Q

R

R

Q

Q

M

M

K

S

G

L

G

P

G

G

T

G

L

I

V

I

T

S

F

F

V

E

V

I

E

A

G

G

K

L

E

E

G

A

A

G

F

R

R

R

F

M

A

I

N

N

A

S

L

V

G

E

L

L

I

C

R

L

I

L

S

A

N

V

N

S

L

L

G

G

D

D

A

T

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

P

F

V

T

A

P

P

E

E

A

E

R

R

A

L

E

K

M

A

G

G

I

I

S

T

D

D

G

G

L

L

L

I

R

R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

P

D

E

D

D

L

I

I

I

A

N

D

D

L

L

A

E

T

H

A

A

active site: Y101 (≠ F124), D173 (= D196), K198 (= K221)
binding [5-hydroxy-4-(iminomethyl)-6-methyl-pyridin-3-yl]methyl dihydrogen phosphate: G76 (= G99), I77 (≠ M100), Y101 (≠ F124), E144 (= E167), D173 (= D196), F176 (= F199), S195 (= S218), T197 (= T220), K198 (= K221)

5dx5A Crystal structure of methionine gamma-lyase from clostridium sporogenes (see paper)
41% identity, 94% coverage: 23:402/406 of query aligns to 10:393/399 of 5dx5A

query
sites
5dx5A

T

T

R

K

L

A

V

I

H

H

G

G

T

H

T

I

-

G

R

D

S

K

P

Q

Y

F

G

G

E

S

T

L

S

A

E

T

A

P

L

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

I

Y

F

D

D

N

S

A

A

E

E

S

Q

C

G

E

G

A

R

R

R

F

F

K

A

G

G

E

E

D

E

P

S

G

G

F

Y

V

I

Y
|
Y

S

S

R
|
R

Y

L

G

G

N

N

P

P

T

T

T

S

A

T

M

E

F

V

E

E

E

N

R

K

M

L

A

A

L

L

E

E

G

G

A

G

E

E

A

A

R

V

A

V

T

A

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

N

A

A

A

A

S

L

L

L

W

C

S

Q

A

L

L

S

K

A

S

G

G

D

D

H

H

V

V

A

A

A

S

R

D

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

F

W

A

I

L

L

L

E

N

D

H

F

G

L

L

P

T

R

R

F

Y

G

G

I

V

E

E

T

V

T

T

L

F

V

V

D

D

G

V

T

S

D

N

L

L

A

D

A

E

W

V

Q

K

A

N

A

A

A

L

R

K

P

P

S

N

T

T

K

K

V

V

F

V

F

Y

L

L

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

K

L

V

I

T

D

D

I

I

A

R

A

K

V

I

A

S

Q

N

I

M

A

V

H

H

A

E

A

S

G

N

A

K

R

E

-

C

-

F

L

V

V

F

V

V

D
|
D

N

N

V

T

F
|
F

A

C

T

T

P

P

L

Y

L

I

Q

Q

H

R

P

P

F

L

E

E

L

L

G

G

A

A

D

D

I

V

V

V

Y

H

S
|
S

T

A

T
|
T

K
|
K

H

Y

V

L

D

N

G

G

Q

H

G

G

R

D

C

V

L

I

G

A

G

G

V

F

I

A

L

V

S

G

S

K

Q

E

F

F

V

I

T

N

D

Q

-

V

H

K

L

L

Q

F

T

G

F

I

L

K

R

D

Q

M

T

T

G

G

P

S

S

V

I

T

S

G

P

P

F

F

N

E

A

S

W

F

I

L

M

I

L

I

K

R

G

G

L

M

E

K

T

T

L

L

A

Q

L

L

R

R

V

M

E

E

R

K

M

H

Q

C

A

K

S

N

A

A

G

M

R

E

I

V

A

A

D

K

A

F

L

L

A

E

G

S

L

H

P

P

K

A

I

V

E

E

T

K

V

V

L

Y

Y

Y

P

P

G

G

R

L

A

E

D

S

H

F

P

K

Q

Y

A

Y

E

Q

L

L

A

A

R

R

R

E

Q

Q

M

M

K

K

G

L

G

P

G

G

T

A

L

M

V

I

T

S

F

F

V

E

V

L

E

K

G

G

K

V

E

E

G

E

A

G

F

K

R

I

F

V

A

M

N

N

A

N

L

V

G

K

L

L

I

A

R

T

I

L

S

A

N

V

N

S

L

L

G

G

D

D

A

S

K

E

S

T

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

P

F

Y

T

T

P

A

E

E

A

E

R

R

A

K

E

A

M

A

G

G

I

I

S

S

D

D

G

G

L

L

L

V

R

R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

A

D

E

D

D

L

I

I

I

A

D

D

D

L

L

A

K

T

Q

A

A

active site: R59 (= R71), Y112 (≠ F124), D186 (= D196), K211 (= K221)
binding pyridoxal-5'-phosphate: Y57 (= Y69), R59 (= R71), S86 (= S98), G87 (= G99), M88 (= M100), Y112 (≠ F124), D186 (= D196), F189 (= F199), S208 (= S218), T210 (= T220), K211 (= K221)

5x30C Crystal structure of pseudomonas putida methionine gamma-lyase c116h mutant with l-homocysteine intermediates. (see paper)
44% identity, 94% coverage: 23:402/406 of query aligns to 7:388/393 of 5x30C

query
sites
5x30C

T

T

R

R

L

A

V

I

H

H

G

H

G

G

T

Y

T

D

R

P

S

Q

P

D

Y

H

G

G

-

G

E

A

T

L

S

V

E

P

A

P

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

N

T

A

V

E

E

S

Y

C

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

D

Q

P

A

G

G

F

H

V

F

Y
|
Y

S

S

R
|
R

Y

I

G

S

N

N

P

P

T

T

T

L

A

N

M

L

F

L

E

E

E

A

R

R

M

M

A

A

L

S

L

L

E

E

G

G

A

G

E

E

A

A

A

G

R

L

A

A

T

L

A

A

S

S

G

G

M

M

A

G

A

A

V

I

N

T

A

S

A

T

L

L

L

W

C

T

Q

L

L

L

S

R

A

P

G

G

D

D

H

E

V

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

H

C

T

R

F

W

A

I

F

L

L

E

H

D

H

F

G

L

I

P

G

R

E

F

F

G

G

I

V

E

K

T

L

T

R

L

H

V

V

D

D

G

M

T

A

D

D

L

L

A

Q

A

A

W

L

Q

E

A

A

A

M

R

T

P

P

S

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

L

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

Q

K

I

I

A

A

H

R

A

K

A

H

G

G

A

A

R

T

L

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

L

L

Q

Q

H

R

P

P

F

L

E

E

L

L

G

G

A

A

D

D

I

L

V

V

Y

H

S

S

T

A

T

T

K
|
K

H

Y

V

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

S

S

Q

Q

Q

A

F

L

V

V

T

D

D

R

-

I

H

R

L

L

Q

Q

T

G

F

L

L

K

R

D

Q

M

T

T

G

G

P

A

S

V

I

L

S

S

P

P

F

H

N

D

A

A

W

A

I

L

M

L

L

M

K

R

G

G

L

I

E

K

T

T

L

L

A

N

L

L

R

R

V

M

E

D

R

R

M

H

Q

C

A

A

S

N

A

A

G

Q

R

V

I

L

A

A

D

E

A

F

L

L

A

A

G

R

L

Q

P

P

K

Q

I

V

E

E

T

L

V

I

L

H

Y

Y

P

P

G

G

R

L

A

A

D

S

H

F

P

P

Q

Q

A

Y

E

T

L

L

A

A

R

R

R

Q

Q

Q

M

M

K

S

G

Q

G

P

G

G

T

G

L

M

V

I

T

A

F

F

V

E

V

L

E

K

G

G

K

I

E

G

G

A

A

G

F

R

R

R

F

F

A

M

N

N

A

A

L

L

G

Q

L

L

I

F

R

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

A

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

S

F

Y

T

T

P

P

E

E

A

E

R

R

A

A

E

H

M

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R

R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

I

D

D

L

L

I

L

A

A

D

D

L

V

A

Q

T

Q

A

A

active site: R56 (= R71), Y109 (≠ F124), D181 (= D196), K206 (= K221)
binding (2~{S})-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]-4-sulfanyl-butanoic acid: Y54 (= Y69), R56 (= R71)

5x2xA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-homocysteine intermediates (see paper)
44% identity, 94% coverage: 23:402/406 of query aligns to 6:387/392 of 5x2xA

query
sites
5x2xA

T

T

R

R

L

A

V

I

H

H

G

H

G

G

T

Y

T

D

R

P

S

Q

P

D

Y

H

G

G

-

G

E

A

T

L

S

V

E

P

A

P

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

N

T

A

V

E

E

S

Y

C

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

D

Q

P

A

G

G

F

H

V

F

Y
|
Y

S

S

R
|
R

Y

I

G

S

N

N

P

P

T

T

T

L

A

N

M

L

F

L

E

E

E

A

R

R

M

M

A

A

L

S

L

L

E

E

G

G

A

G

E

E

A

A

A

G

R

L

A

A

T

L

A

A

S

S

G
|
G

M
|
M

A

G

A

A

V

I

N

T

A

S

A

T

L

L

L

W

C

T

Q

L

L

L

S

R

A

P

G

G

D

D

H

E

V

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

F

W

A

I

F

L

L

E

H

D

H

F

G

L

I

P

G

R

E

F

F

G

G

I

V

E

K

T

L

T

R

L

H

V

V

D

D

G

M

T

A

D

D

L

L

A

Q

A

A

W

L

Q

E

A

A

A

M

R

T

P

P

S

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N
|
N

P

P

T

N

L

M

E

H

L

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

Q

K

I

I

A

A

H

R

A

K

A

H

G

G

A

A

R

T

L

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

L

L

Q

Q

H

R

P

P

F

L

E

E

L

L

G

G

A

A

D

D

I

L

V

V

Y

H

S
|
S

T

A

T
|
T

K
|
K

H

Y

V

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

S

S

Q

Q

Q

A

F

L

V

V

T

D

D

R

-

I

H

R

L

L

Q

Q

T

G

F

L

L

K

R

D

Q

M

T

T

G

G

P

A

S

V

I

L

S

S

P

P

F

H

N

D

A

A

W

A

I

L

M

L

L

M

K

R

G

G

L

I

E

K

T

T

L

L

A

N

L

L

R

R

V

M

E

D

R

R

M

H

Q

C

A

A

S

N

A

A

G

Q

R

V

I

L

A

A

D

E

A

F

L

L

A

A

G

R

L

Q

P

P

K

Q

I

V

E

E

T

L

V

I

L

H

Y

Y

P

P

G

G

R

L

A

A

D

S

H

F

P

P

Q

Q

A

Y

E

T

L

L

A

A

R

R

R

Q

Q

Q

M

M

K

S

G

Q

G

P

G

G

T

G

L

M

V

I

T

A

F

F

V

E

V

L

E

K

G

G

K

I

E

G

G

A

A

G

F

R

R

R

F

F

A

M

N

N

A

A

L

L

G

Q

L

L

I

F

R

S

I

R

S

A

N
x
V

N
x
S

L

L

G

G

D

D

A

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

S

F

Y

T

T

P

P

E

E

A

E

R

R

A

A

E

H

M

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

I

D

D

L

L

I

L

A

A

D

D

L

V

A

Q

T

Q

A

A

active site: R55 (= R71), Y108 (≠ F124), D180 (= D196), K205 (= K221)
binding (2E)-2-{[(1E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}but-2-enoic acid: Y53 (= Y69), R55 (= R71), G83 (= G99), M84 (= M100), Y108 (≠ F124), N155 (= N171), D180 (= D196), S202 (= S218), T204 (= T220), K205 (= K221), V333 (≠ N348), S334 (≠ N349), R369 (= R384)

5x2wA Crystal structure of pseudomonas putida methionine gamma-lyase wild type with l-methionine intermediates (see paper)
44% identity, 94% coverage: 23:402/406 of query aligns to 6:387/392 of 5x2wA

query
sites
5x2wA

T

T

R

R

L

A

V

I

H

H

G

H

G

G

T

Y

T

D

R

P

S

Q

P

D

Y

H

G

G

-

G

E

A

T

L

S

V

E

P

A

P

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

N

T

A

V

E

E

S

Y

C

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

D

Q

P

A

G

G

F

H

V

F

Y
|
Y

S

S

R
|
R

Y

I

G

S

N

N

P

P

T

T

T

L

A

N

M

L

F

L

E

E

E

A

R

R

M

M

A

A

L

S

L

L

E

E

G

G

A

G

E

E

A

A

A

G

R

L

A

A

T

L

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

N

T

A

S

A

T

L

L

L

W

C

T

Q

L

L

L

S

R

A

P

G

G

D

D

H

E

V

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

C

C

T

R

F

W

A

I

F

L

L

E

H

D

H

F

G

L

I

P

G

R

E

F

F

G

G

I

V

E

K

T

L

T

R

L

H

V

V

D

D

G

M

T

A

D

D

L

L

A

Q

A

A

W

L

Q

E

A

A

A

M

R

T

P

P

S

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

L

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

Q

K

I

I

A

A

H

R

A

K

A

H

G

G

A

A

R

T

L

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

L

Y

L

L

Q

Q

H

R

P

P

F

L

E

E

L

L

G

G

A

A

D

D

I

L

V

V

Y

H

S
|
S

T

A

T

T

K
|
K

H

Y

V

L

D

S

G

G

Q

H

G

G

R

D

C

I

L

T

G

A

G

G

V

I

I

V

L

V

S

G

S

S

Q

Q

Q

A

F

L

V

V

T

D

D

R

-

I

H

R

L

L

Q

Q

T

G

F

L

L

K

R

D

Q

M

T

T

G

G

P

A

S

V

I

L

S

S

P

P

F

H

N

D

A

A

W

A

I

L

M

L

L

M

K

R

G

G

L

I

E

K

T

T

L

L

A

N

L

L

R

R

V

M

E

D

R

R

M

H

Q

C

A

A

S

N

A

A

G

Q

R

V

I

L

A

A

D

E

A

F

L

L

A

A

G

R

L

Q

P

P

K

Q

I

V

E

E

T

L

V

I

L

H

Y

Y

P

P

G

G

R

L

A

A

D

S

H

F

P

P

Q

Q

A

Y

E

T

L

L

A

A

R

R

R

Q

Q

Q

M

M

K

S

G

Q

G

P

G

G

T

G

L

M

V

I

T

A

F

F

V

E

V

L

E

K

G

G

K

I

E

G

G

A

A

G

F

R

R

R

F

F

A

M

N

N

A

A

L

L

G

Q

L

L

I

F

R

S

I

R

S

A

N
x
V

N
x
S

L

L

G

G

D

D

A

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

S

F

Y

T

T

P

P

E

E

A

E

R

R

A

A

E

H

M

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

P

I

D

D

L

L

I

L

A

A

D

D

L

V

A

Q

T

Q

A

A

active site: R55 (= R71), Y108 (≠ F124), D180 (= D196), K205 (= K221)
binding (2E)-2-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]-4-(methylsulfanyl)but-2-enoic acid: Y53 (= Y69), R55 (= R71), S82 (= S98), G83 (= G99), M84 (= M100), Y108 (≠ F124), D180 (= D196), S202 (= S218), K205 (= K221), V333 (≠ N348), S334 (≠ N349), R369 (= R384)

1pg8A Crystal structure of l-methionine alpha-, gamma-lyase
44% identity, 94% coverage: 23:402/406 of query aligns to 12:393/398 of 1pg8A

query
sites
1pg8A

T

T

R

R

L

A

V

I

H

H

G

H

G

G

T

Y

T

D

R

P

S

Q

P

D

Y

H

G

G

-

G

E

A

T

L

S

V

E

P

A

P

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

N

T

A

V

E

E

S

Y

C

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

D

Q

P

A

G

G

F

H

V

F

Y
|
Y

S

S

R
|
R

Y

I

G

S

N

N

P

P

T

T

T

L

A

N

M

L

F

L

E

E

E

A

R

R

M

M

A

A

L

S

L

L

E

E

G

G

A

G

E

E

A

A

A

G

R

L

A

A

T

L

A

A

S
|
S

G
|
G

M
|
M

A

G

A

A

V

I

N

T

A

S

A

T

L

L

L

W

C

T

Q

L

L

L

S

R

A

P

G

G

D

D

H

E

V

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

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C

C

T

R

F

W

A

I

F

L

L

E

H

D

H

F

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F

F

G

G

I

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E

K

T

L

T

R

L

H

V

V

D

D

G

M

T

A

D

D

L

L

A

Q

A

A

W

L

Q

E

A

A

A

M

R

T

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P

S

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

L

M

I

A

D

D

I

I

A

A

A

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A

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I

I

A

A

H

R

A

K

A

H

G

G

A

A

R

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L

V

V

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|
D

N

N

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Y

A

C

T

T

P

P

L

Y

L

L

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Q

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R

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P

F

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E

E

L

L

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G

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A

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D

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I

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T

D

D

R

-

I

H

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D

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T

T

L

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N

L

L

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E

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C

A

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A

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A

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A

F

L

L

A

A

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V

I

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H

Y

Y

P

P

G

G

R

L

A

A

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S

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P

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Q

A

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E

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L

A

A

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Q

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P

G

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T

G

L

M

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I

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A

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F

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E

V

L

E

K

G

G

K

I

E

G

G

A

A

G

F

R

R

R

F

F

A

M

N

N

A

A

L

L

G

Q

L

L

I

F

R

S

I

R

S

A

N

V

N

S

L

L

G

G

D

D

A

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

S

F

Y

T

T

P

P

E

E

A

E

R

R

A

A

E

H

M

Y

G

G

I

I

S

S

D

E

G

G

L

L

L

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R

R

L

L

S

S

V

V

G

G

L

L

E

E

H

D

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I

D

D

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A

A

D

D

L

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A

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T

Q

A

A

active site: R61 (= R71), Y114 (≠ F124), D186 (= D196), K211 (= K221)
binding pyridoxal-5'-phosphate: Y59 (= Y69), R61 (= R71), S88 (= S98), G89 (= G99), M90 (= M100), Y114 (≠ F124), D186 (= D196), S208 (= S218), T210 (= T220), K211 (= K221)

P13254 L-methionine gamma-lyase; MGL; Homocysteine desulfhydrase; L-methioninase; EC 4.4.1.11; EC 4.4.1.2 from Pseudomonas putida (Arthrobacter siderocapsulatus) (see 3 papers)
44% identity, 94% coverage: 23:402/406 of query aligns to 12:393/398 of P13254

query
sites
P13254

T

T

R

R

L

A

V

I

H

H

G

H

G

G

T

Y

T

D

R

P

S

Q

P

D

Y

H

G

G

-

G

E

A

T

L

S

V

E

P

A

P

L

V

F

Y

L

Q

T

T

Q

A

G

T

F

F

V

T

Y

F

D

P

N

T

A

V

E

E

S

Y

C

G

E

A

A

A

R

C

F

F

K

A

G

G

E

E

D

Q

P

A

G

G

F

H

V

F

Y
|
Y

S
|
S

R
|
R

Y

I

G

S

N

N

P

P

T

T

T

L

A

N

M

L

F

L

E

E

E

A

R

R

M

M

A

A

L

S

L

L

E

E

G

G

A

G

E

E

A

A

A

G

R

L

A

A

T

L

A

A

S

S

G
|
G

M
|
M

A

G

A

A

V

I

N

T

A

S

A

T

L

L

L

W

C

T

Q

L

L

L

S

R

A

P

G

G

D

D

H

E

V

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S
x
C

C

T

R

F

W

A

I

F

L

L

E

H

D

H

F

G

L

I

P

G

R

E

F

F

G

G

I

V

E

K

T

L

T

R

L

H

V

V

D

D

G

M

T

A

D

D

L

L

A

Q

A

A

W

L

Q

E

A

A

A

M

R

T

P

P

S

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

L

M

E

H

L

M

I

A

D

D

I

I

A

A

A

G

V

V

A

A

Q

K

I

I

A

A

H

R

A

K

A

H

G

G

A

A

R

T

L

V

V

V

D

D

N

N

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T

F

Y

A

C

T

T

P

P

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Y

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L

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Q

H

R

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P

F

L

E

E

L

L

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G

A

A

D

D

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|
S

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x
A

T
|
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|
K

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G

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H

G

G

R

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C

I

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T

G

A

G

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L

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D

R

-

I

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R

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L

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Q

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x
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T

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A

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E

K

G

G

K

I

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G

G

A

A

G

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F

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M

N

N

A

A

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L

L

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F

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I

R

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A

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N

S

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D

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A

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M

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Q

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S

F

Y

T

T

P

P

E

E

A

E

R

R

A

A

E

H

M

Y

G

G

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G

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L

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|
R

L

L

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S

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V

G

G

L

L

E

E

H

D

P

I

D

D

L

L

I

L

A

A

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D

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T

Q

A

A

YSR 59:61 (= YSR 69:71) binding pyridoxal 5'-phosphate
R61 (= R71) mutation R->A,E,F: Loss of elimination activity against L-methionine.
GM 89:90 (= GM 99:100) binding in other chain
Y114 (≠ F124) binding substrate
C116 (≠ S126) mutation to H: Drastic decrease of the catalytic efficiency of the elimination reaction with L-methionine, by 6700-fold, and increases that with L-cysteine by 7-fold, mainly due to changes in kcat. Loss of ability to catalyze replacement reaction between L-methionine and 2-mercaptoethanol.; mutation to S: 9% of wild-type elimination activity against L-methionine.; mutation to T: 40% of wild-type elimination activity against L-methionine.
SAT 208:210 (≠ STT 218:220) binding in other chain
K211 (= K221) modified: N6-(pyridoxal phosphate)lysine
K240 (≠ L249) mutation K->D,E: Marked decrease in elimination activity against both L-methionine and DL-homocysteine.; mutation to M: 50% reduction in alpha,gamma-elimination activity against DL-homocysteine, while retaining elimination activity against L-methionine and L-cysteine.
D241 (≠ R250) mutation D->H,R: 5 to 14-fold reduction in alpha,gamma-elimination activity against L-methionine, while no change in affinity for L-methionine.
R375 (= R384) binding substrate

3vk3A Crystal structure of l-methionine gamma-lyase from pseudomonas putida c116h mutant complexed with l-methionine (see paper)
44% identity, 94% coverage: 23:402/406 of query aligns to 11:392/397 of 3vk3A

query
sites
3vk3A

T

T

R

R

L

A

V

I

H

H

G

H

G

G

T

Y

T

D

R

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D

Y

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G

G

-

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E

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T

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E

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A

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F

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F

D

P

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T

A

V

E

E

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C

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E

A

A

A

R

C

F

F

K

A

G

G

E

E

D

Q

P

A

G

G

F

H

V

F

Y

Y

S

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R
|
R

Y

I

G

S

N

N

P

P

T

T

T

L

A

N

M

L

F

L

E

E

E

A

R

R

M

M

A

A

L

S

L

L

E

E

G

G

A

G

E

E

A

A

A

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R

L

A

A

T

L

A

A

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S

G

G

M

M

A

G

A

A

V

I

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T

A

S

A

T

L

L

L

W

C

T

Q

L

L

L

S

R

A

P

G

G

D

D

H

E

V

V

V

L

A

L

A

G

R

N

A

T

L

L

F
x
Y

G

G

S

H

C

T

R

F

W

A

I

F

L

L

E

H

D

H

F

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I

P

G

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E

F

F

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G

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E

K

T

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T

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V

D

D

G

M

T

A

D

D

L

L

A

Q

A

A

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L

Q

E

A

A

A

M

R

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P

P

S

A

T

T

K

R

V

V

F

I

F

Y

L

F

E

E

S

S

P

P

T

A

N

N

P

P

T

N

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M

E

H

L

M

I

A

D

D

I

I

A

A

A

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V

A

A

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I

I

A

A

H

R

A

K

A

H

G

G

A

A

R

T

L

V

V

V

D
|
D

N

N

V

T

F

Y

A

C

T

T

P

P

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Y

L

L

Q

Q

H

R

P

P

F

L

E

E

L

L

G

G

A

A

D

D

I

L

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V

Y

H

S

S

T

A

T

T

K
|
K

H

Y

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G

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G

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D

C

I

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T

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A

G

G

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I

I

V

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V

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G

S

S

Q

Q

Q

A

F

L

V

V

T

D

D

R

-

I

H

R

L

L

Q

Q

T

G

F

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L

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R

D

Q

M

T

T

G

G

P

A

S

V

I

L

S

S

P

P

F

H

N

D

A

A

W

A

I

L

M

L

L

M

K

R

G

G

L

I

E

K

T

T

L

L

A

N

L

L

R

R

V

M

E

D

R

R

M

H

Q

C

A

A

S

N

A

A

G

Q

R

V

I

L

A

A

D

E

A

F

L

L

A

A

G

R

L

Q

P

P

K

Q

I

V

E

E

T

L

V

I

L

H

Y

Y

P

P

G

G

R

L

A

A

D

S

H

F

P

P

Q

Q

A

Y

E

T

L

L

A

A

R

R

R

Q

Q

Q

M

M

K

S

G

Q

G

P

G

G

T

G

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M

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I

T

A

F

F

V

E

V

L

E

K

G

G

K

I

E

G

G

A

A

G

F

R

R

R

F

F

A

M

N

N

A

A

L

L

G

Q

L

L

I

F

R

S

I

R

S

A

N
x
V

N
x
S

L
|
L

G

G

D

D

A

A

K

E

S

S

L

L

I

A

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

S

R

S

F

Y

T

T

P

P

E

E

A

E

R

R

A

A

E

H

M

Y

G

G

I

I

S

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E

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G

L

L

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|
R

L

L

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S

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V

G

G

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L

E

E

H

D

P

I

D

D

L

L

I

L

A

A

D

D

L

V

A

Q

T

Q

A

A

active site: R60 (= R71), Y113 (≠ F124), D185 (= D196), K210 (= K221)
binding methionine: Y113 (≠ F124), K210 (= K221), V338 (≠ N348), S339 (≠ N349), L340 (= L350), R374 (= R384)

1e5fA Methionine gamma-lyase (mgl) from trichomonas vaginalis
36% identity, 95% coverage: 21:406/406 of query aligns to 5:392/393 of 1e5fA

query
sites
1e5fA

P

P

E

A

T

T

R

A

L

C

V

I

H

H

G

A

G

N

T

P

T

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F

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A

T

A

S

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P

A

P

L

I

F

Y

L

Q

T

T

Q

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T

F

F

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V

Y

F

D

D

N

N

A

C

E

Q

S

Q

C

G

E

G

A

N

R

R

F

F

K

A

G

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E

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G

G

F

Y

V

I

Y
|
Y

S

T

R
|
R

Y

L

G

G

N

N

P

P

T

T

T

V

A

S

M

N

F

L

E

E

E

G

R

K

M

I

A

A

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F

L

L

E

E

G

K

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E

E

A

A

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A

A

T

T

A

S

S

S

G
|
G

M
|
M

A

G

A

A

V

I

N

A

A

A

A

T

L

V

L

L

C

T

Q

I

L

L

S

K

A

A

G

G

D

D

H

H

V

L

V

I

A

S

A

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E

A

C

L

L

F
x
Y

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G

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C

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W

A

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E

E

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H

F

A

L

L

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K

F

F

G

G

I

I

E

Q

T

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T

D

L

F

V

I

D

N

G

T

T

A

D

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A

G

A

E

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K

A

K

A

H

A

M

R

K

P

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T

T

K

K

V

I

F

V

F

Y

L

F

E

E

S

T

P

P

T

A

N

N

P

P

T

T

L

L

E

K

L

I

I

I

D

D

I

M

A

E

A

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V

A

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K

I

D

A

A

H

H

A

S

A

Q

-

E

G

G

A

V

R

L

L

V

V

I

V

A

D
|
D

N

N

V

T

F

F

A

C

T

S

P

P

L

M

L

I

Q

T

H

N

P

P

F

V

E

D

L

F

G

G

A

V

D

D

I

V

V

V

Y

H

S
|
S

T

A

T

T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

T

R

D

C

V

L

V

G

A

G

G

V

L

I

I

L

C

S

G

S

K

Q

A

Q

D

F

L

V

L

T

Q

D

Q

H

I

L

R

Q

M

T

V

F

G

L

I

R

K

Q

D

-

I

T

T

G

G

P

S

S

V

I

I

S

S

P

P

F

H

N

D

A

A

W

W

I

L

M

I

L

T

K

R

G

G

L

L

E

S

T

T

L

L

A

N

L

I

R

R

V

M

E

K

R

A

M

E

Q

S

A

E

S

N

A

A

G

M

R

K

I

V

A

A

D

E

A

Y

L

L

A

K

G

S

L

H

P

P

K

A

I

V

E

E

T

K

V

V

L

Y

Y

Y

P

P

G

G

R

F

A

E

D

D

H

H

P

E

Q

G

A

H

E

D

L

I

A

A

R

K

Q

Q

M

M

K

R

G

M

G

Y

G

G

T

S

L

M

V

I

T

T

F

F

V

I

V

L

E

K

G

S

G

G

K

F

E

E

G

G

A

A

F

K

R

K

F

L

A

L

N

D

A

N

L

L

G

K

L

L

I

I

R

T

I

L

S

A

N

V

N

S

L

L

G

G

D

G

A

C

K

E

S

S

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T

T

H

H

Q

A

R

V

F

V

T

P

P

K

E

E

A

E

R

R

A

E

E

A

M

A

G

G

I

I

S

T

D

D

G

G

L

M

L

I

R

R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

P

A

D

D

D

E

L

L

I

I

A

A

D

D

L

F

A

K

T

Q

A

G

A

L

G

D

A

A

L

L

active site: R55 (= R71), Y108 (≠ F124), D181 (= D196), K206 (= K221)
binding pyridoxal-5'-phosphate: Y53 (= Y69), R55 (= R71), G83 (= G99), M84 (= M100), Y108 (≠ F124), D181 (= D196), S203 (= S218), K206 (= K221)

1e5eA Methionine gamma-lyase (mgl) from trichomonas vaginalis in complex with propargylglycine
36% identity, 95% coverage: 21:406/406 of query aligns to 5:392/394 of 1e5eA

query
sites
1e5eA

P

P

E

A

T

T

R

A

L

C

V

I

H

H

G

A

G

N

T

P

T

Q

R

K

S

D

P

Q

Y

F

G

G

E

A

T

A

S

I

E

P

A

P

L

I

F

Y

L

Q

T

T

Q

S

G

T

F

F

V

V

Y

F

D

D

N

N

A

C

E

Q

S

Q

C

G

E

G

A

N

R

R

F

F

K

A

G

G

E

Q

D

E

P

S

G

G

F

Y

V

I

Y
|
Y

S

T

R
|
R

Y

L

G

G

N

N

P

P

T

T

T

V

A

S

M

N

F

L

E

E

E

G

R

K

M

I

A

A

L

F

L

L

E

E

G

K

A

T

E

E

A

A

A

C

R

V

A

A

T

T

A

S

S

S

G
|
G

M
|
M

A

G

A

A

V

I

N

A

A

A

A

T

L

V

L

L

C

T

Q

I

L

L

S

K

A

A

G

G

D

D

H

H

V

L

V

I

A

S

A

D

R

E

A

C

L

L

F
x
Y

G

G

S

C

C

T

R

H

W

A

I

L

L

F

E

E

D

H

F

A

L

L

P

T

R

K

F

F

G

G

I

I

E

Q

T

V

T

D

L

F

V

I

D

N

G

T

T

A

D

I

L

P

A

G

A

E

W

V

Q

K

A

K

A

H

A

M

R

K

P

P

S

N

T

T

K

K

V

I

F

V

F

Y

L

F

E

E

S

T

P

P

T

A

N
|
N

P

P

T

T

L

L

E

K

L

I

I

I

D

D

I

M

A

E

A

R

V

V

A

C

Q

K

I

D

A

A

H

H

A

S

A

Q

-

E

G

G

A

V

R

L

L

V

V

I

V

A

D
|
D

N

N

V

T

F

F

A

C

T

S

P

P

L

M

L

I

Q

T

H

N

P

P

F

V

E

D

L

F

G

G

A

V

D

D

I

V

V

V

Y

H

S
|
S

T

A

T
|
T

K
|
K

H

Y

V

I

D

N

G

G

Q

H

G

T

R

D

C

V

L

V

G

A

G

G

V

L

I

I

L

C

S

G

S

K

Q

A

Q

D

F

L

V

L

T

Q

D

Q

H

I

L

R

Q

M

T

V

F

G

L

I

R

K

Q

D

-

I

T

T

G

G

P

S

S

V

I

I

S

S

P

P

F

H

N

D

A

A

W

W

I

L

M

I

L

T

K

R

G

G

L

L

E

S

T

T

L

L

A

N

L

I

R

R

V

M

E

K

R

A

M

E

Q

S

A

E

S

N

A

A

G

M

R

K

I

V

A

A

D

E

A

Y

L

L

A

K

G

S

L

H

P

P

K

A

I

V

E

E

T

K

V

V

L

Y

Y

Y

P

P

G

G

R

F

A

E

D

D

H

H

P

E

Q

G

A

H

E

D

L

I

A

A

R

K

Q

Q

M

M

K

R

G

M

G

Y

G

G

T

S

L

M

V

I

T

T

F

F

V

I

V

L

E

K

G

S

G

G

K

F

E

E

G

G

A

A

F

K

R

K

F

L

A

L

N

D

A

N

L

L

G

K

L

L

I

I

R

T

I

L

S

A

N

V

N
x
S

L

L

G

G

D

G

A

C

K

E

S

S

L

L

I

I

T

Q

H

H

P

P

A

A

T

S

T

M

T
|
T

H

H

Q

A

R

V

F

V

T

P

P

K

E

E

A

E

R

R

A

E

E

A

M

A

G

G

I

I

S

T

D

D

G

G

L

M

L

I

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

P

A

D

D

D

E

L

L

I

I

A

A

D

D

L

F

A

K

T

Q

A

G

A

L

G

D

A

A

L

L

active site: R55 (= R71), Y108 (≠ F124), D181 (= D196), K206 (= K221)
binding n-(hydroxy{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)norvaline: Y53 (= Y69), R55 (= R71), G83 (= G99), M84 (= M100), Y108 (≠ F124), N155 (= N171), D181 (= D196), S203 (= S218), T205 (= T220), K206 (= K221), S335 (≠ N349), T350 (= T364), R370 (= R384)

P55217 Cystathionine gamma-synthase 1, chloroplastic; AtCGS1; METHIONINE OVERACCUMULATION 1; O-succinylhomoserine (thiol)-lyase; EC 2.5.1.48 from Arabidopsis thaliana (Mouse-ear cress) (see 4 papers)
36% identity, 98% coverage: 11:406/406 of query aligns to 171:563/563 of P55217

query
sites
P55217

F

F

I

L

P

S

K

S

D

D

-

G

P

S

L

L

T

T

L

V

H

H

P

A

E

G

T

E

R

R

L

L

V

G

H

R

G

G

G

I

T

V

T

T

-

D

-

A

-

I

R

T

S

T

P

P

Y

V

G

V

E

N

T

T

S

S

E

A

A

Y

L

F

F

F

L

K

T

K

Q

T

G

A

F

E

V

L

Y

I

D

D

N

F

A

K

E

E

S

K

C

R

E

S

A

V

R

S

F

F

K

E

G

-

E

-

D

-

P

-

G

-

F

-

V

-

Y

Y

S

G

R

R

Y

Y

G

G

N

N

P

P

T

T

A

V

M

V

F

L

E

E

E

D

R

K

M

I

A

S

L

A

L

L

E

E

G

G

A

A

E

E

A

S

A

T

R

L

A

V

T

M

A

A

S

S

G

G

M

M

A

C

A

A

V

S

N

T

A

V

A

M

L

L

C

A

Q

L

L

V

S

P

A

A

G

G

D

G

H

H

V

I

V

V

A

T

R

T

A

D

L

C

F

Y

G

R

S

K

C

T

R

R

W

I

I

F

L

M

E

E

D

N

F

F

L

L

P

P

R

K

F

L

G

G

I

I

E

T

T

V

T

T

L

V

V

I

D

D

G

P

T

A

D

D

L

I

A

A

A

G

W

L

Q

E

A

A

A

V

R

N

P

E

-

F

S

K

T

V

K

S

V

L

F

F

L

T

E

E

S

S

P

P

T

T

N

N

P

P

T

F

L

L

E

R

L

C

I

V

D

D

I

I

A

E

A

L

V

V

A

S

Q

K

I

I

A

C

H

H

A

K

A

R

G

G

A

T

R

L

L

V

V

C

V

I

D

D

N

G

V

T

F

F

A

A

T

T

P

P

L

L

L

N

Q

Q

H

K

P

A

F

L

E

A

L

L

G

G

A

A

D

D

I

L

V

V

Y

H

S

S

T

A

T

T

K

K

H

Y

V

I

D

G

G

G

Q

H

G

N

R

D

C

V

L

L

G

A

G

G

V

C

I

I

L

C

S

G

S

S

Q

L

Q

K

F

L

V

V

T

S

D

E

H

-

L

I

Q

R

T

N

F

L

L

H

R

H

Q

V

T

L

G

G

P

G

S
x
T

I

L

S

N

P

P

F

N

N

A

A

A

W

Y

I

L

M

I

L

I

K

R

G

G

L

M

E

K

T

T

L

L

A

H

L

L

R

R

V

V

E

Q

R

Q

M

Q

Q

N

A

S

S

T

A

A

G

F

R

R

I

M

A

A

D

E

A

I

L

L

A

E

G

A

L

H

P

P

K

K

I

V

E

S

T

H

V

V

L

Y

Y

Y

P

P

G
|
G

R

L

A

P

D

S

H

H

P

P

Q

E

A

H

E

E

L

L

A

A

R

K

R

R

Q

Q

M

M

K

T

G

G

G

F

G

G

T

G

L

V

V

V

T

S

F

F

V

E

V

I

E

D

G

G

G

D

K

I

E

E

G

T

A

T

F

I

R

K

F

F

A

V

N

D

A

S

L

L

G

K

L

I

I

P

R

Y

I

I

S

A

N

P

N

S

L

F

G

G

D

G

A

C

K

E

S

S

L

I

I

V

T

D

H

Q

P

P

A

A

T

I

T

M

T

S

H

Y

Q

W

R

D

F

L

T

P

P

Q

E

E

A

E

R

R

A

L

E

K

M

Y

G

G

I

I

S

K

D

D

G

N

L

L

L

V

R

R

L

F

S

S

V

F

G

G

L

V

E

E

H

D

P

F

D

E

D

D

L

V

I

K

A

A

D

D

L

I

A

L

T

Q

A

A

A

L

G

E

A

A

L

I

T412 (≠ S255) natural variant: T -> P
G459 (= G302) natural variant: G -> A

Sites not aligning to the query:

8 natural variant: C -> S
55 natural variant: A -> G
77 R→H: In mto1-4; over-accumulation of soluble methionine.
78 R→K: In mto1-7; over-accumulation of soluble methionine.
81 S→N: In mto1-2; over-accumulation of soluble methionine.
84 G→D: In mto1-3 and mto1-5; over-accumulation of soluble methionine.; G→S: In mto1-1; over-accumulation of soluble methionine.
86 A→V: In mto1-6; over-accumulation of soluble methionine.
91 natural variant: A -> G

4iyoD Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
41% identity, 94% coverage: 23:402/406 of query aligns to 6:379/384 of 4iyoD

query
sites
4iyoD

T

T

R

L

L

A

V

I

H

H

G

G

T

Q

T

S

R

P

S

D

P

P

Y

S

-

T

G

G

E

A

T

V

S

M

E

P

A

P

L

I

F

Y

L

A

T

T

Q

S

G

T

F

Y

V

A

Y

Q

D

S

N

S

A

-

E

-

S

-

C

-

E

-

A

-

R

-

F

-

K

P

G

G

E

E

D

H

P

Q

G

G

F

F

V

E

Y
|
Y

S

S

R
|
R

Y
x
T

G

H

N

N

P

P

T

T

T

R

A

F

M

A

F

Y

E

E

E

R

R

C

M

V

A

A

L

A

L

L

E

E

G

G

A

G

E

T

A

R

A

A

R

F

A

A

T

F

A

A

S

S

G

G

M

M

A

A

V

-

N

T

A

S

A

T

L

V

L

M

C

E

Q

L

L

L

S

D

A

A

G

G

D

S

H

H

V

V

A

A

A

M

R

D

A

D

L

L

F
x
Y

G

G

S

G

C

T

R

F

W
x
R

I

L

L

F

E

E

D

R

F

V

L

R

P

R

R

R

F

T

-

A

G

G

I

L

E

D

T

F

T

S

L

F

V

V

D

D

G

L

T

T

D

D

L

P

A

A

W

F

Q

K

A

A

A

I

R

R

P

A

S

D

T

T

K

K

V

M

F

V

F

W

L

I

E

E

S

T

P

P

T

T

N

N

P

P

T

M

L

L

E

K

L

L

I

V

D

D

I

I

A

A

V

I

A

A

Q

V

I

I

A

A

H

R

A

K

A

H

G

G

A

L

R

L

L

T

V

V

D
|
D

N

N

V

T

F

F

A

A

T

S

P

P

L

M

L

L

Q

Q

H

R

P

P

F

L

E

S

L

L

G

G

A

A

D

D

I

L

V

V

Y

H

S

S

T

A

T

T

K
|
K

H

Y

V

L

D

N

G

G

Q

H

G

S

R

D

C

M

L

V

G

G

V

I

I

A

L

V

S

V

S

G

Q

D

Q

N

F

A

V

E

T

L

D

A

H

E

L

Q

Q

M

T

A

F

F

L

L

R

Q

Q
x
N

T

S

-

I

G

G

P

G

S

V

I

Q

S

G

P

P

F

F

N

D

A

S

W

F

I

L

M

A

L

L

K

R

G

G

L

L

E

K

T

T

L

L

A

P

L

L

R

R

V

M

E

R

R

A

M

H

Q

C

A

E

S

N

A

A

G

L

R

A

I

L

A

A

D

Q

A

W

L

L

A

E

G

T

L

H

P

P

K

A

I

I

E

E

T

K

V

V

L

I

Y

Y

P

P

G

G

R

L

A

A

D

S

H

H

P

P

Q

Q

A

H

E

V

L

L

A

A

R

K

R

R

Q

Q

M

M

K

S

G

G

G

F

G

G

T

G

L

I

V

V

T

S

F

I

V

V

V

L

E

K

G

G

K

F

E

D

G

A

A

A

F

K

R

R

F

F

A

C

N

E

A

K

L

T

G

E

L

L

I

F

R

T

I

L

S

A

N
x
E

N
x
S

L

L

G

G

D

G

A

V

K

E

S

S

L

L

I

V

T

N

H

H

P

P

A

A

T

V

T

M

T
|
T

H

H

Q

A

R

S

F

I

T

P

P

V

E

A

A

R

R

R

A

E

E

Q

M

L

G

G

I

I

S

S

D

D

G

A

L

L

L

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

H

D

P

L

D

G

D

D

L

L

I

R

A

G

D

D

L

L

A

E

T

R

A

A

active site: R47 (= R71), Y99 (≠ F124), D172 (= D196), K197 (= K221)
binding serine: Y45 (= Y69), T48 (≠ Y72), Y99 (≠ F124), Y99 (≠ F124), R104 (≠ W129), K197 (= K221), N227 (≠ Q251), E325 (≠ N348), S326 (≠ N349), T341 (= T364), R361 (= R384)

Query Sequence

>WP_011996129.1 NCBI__GCF_000017645.1:WP_011996129.1
MSVAQKPAPKFIPKDPLTLHPETRLVHGGTTRSPYGETSEALFLTQGFVYDNAESCEARF
KGEDPGFVYSRYGNPTTAMFEERMALLEGAEAARATASGMAAVNAALLCQLSAGDHVVAA
RALFGSCRWILEDFLPRFGIETTLVDGTDLAAWQAAARPSTKVFFLESPTNPTLELIDIA
AVAQIAHAAGARLVVDNVFATPLLQHPFELGADIVVYSTTKHVDGQGRCLGGVILSSQQF
VTDHLQTFLRQTGPSISPFNAWIMLKGLETLALRVERMQASAGRIADALAGLPKIETVLY
PGRADHPQAELARRQMKGGGTLVTFVVEGGKEGAFRFANALGLIRISNNLGDAKSLITHP
ATTTHQRFTPEARAEMGISDGLLRLSVGLEHPDDLIADLATAAGAL

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory