SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012035876.1 NCBI__GCF_000063445.1:WP_012035876.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O58056 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
54% identity, 98% coverage: 3:418/426 of query aligns to 2:420/430 of O58056

query
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O58056

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R118 (= R118) binding ATP
K159 (= K158) binding ATP
EEHI 199:202 (≠ EEFI 197:200) binding ATP
E207 (= E205) binding ATP

2dwcB Crystal structure of probable phosphoribosylglycinamide formyl transferase from pyrococcus horikoshii ot3 complexed with adp
52% identity, 98% coverage: 3:418/426 of query aligns to 4:409/409 of 2dwcB

query
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2dwcB

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active site: E265 (= E279), E277 (= E293), D284 (= D300), T285 (= T301), R372 (= R382)
binding adenosine-5'-diphosphate: R120 (= R118), H159 (≠ V156), K161 (= K158), H190 (≠ F199), I191 (= I200), F193 (= F202), E196 (= E205), F267 (= F281), E277 (= E293)

1kjiA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-amppcp (see paper)
44% identity, 95% coverage: 8:410/426 of query aligns to 2:388/389 of 1kjiA

query
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1kjiA

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active site: E114 (= E119), K154 (= K158), S159 (= S163), G161 (= G165), E264 (= E279), E276 (= E293), D283 (= D300), T284 (= T301), R360 (= R382)
binding phosphomethylphosphonic acid adenylate ester: R113 (= R118), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (≠ S168), E192 (= E197), V195 (≠ I200), E200 (= E205), Q222 (= Q232), E264 (= E279), F266 (= F281), E276 (= E293)
binding magnesium ion: E264 (= E279), E276 (= E293)

1ez1A Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg, amppnp, and gar (see paper)
44% identity, 95% coverage: 8:410/426 of query aligns to 2:388/389 of 1ez1A

query
sites
1ez1A

V

L

L

L

G

G

T

T

P

A

Y

L

A

R

N

P

G

A

A

A

-

T

K

R

L

V

M

M

F

L

L

L

G

G

A

S

G
|
G

E
|
E

L
|
L

G

G

K

K

E

E

T

V

M

A

I

I

E

E

A

C

Q

Q

R

R

M

L

G

G

I

V

E

E

I

V

V

I

A

A

V

V

D

D

R

R

Y

Y

A

A

N

D

S

A

P

P

G

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

T

I

N

N

M

M

K

L

S

D

E

G

R

D

A

A

L

L

L

R

A

R

I

V

V

V

E

E

K

L

E

E

K

K

P

P

D

H

A

Y

I

I

I

V

P

P

E
|
E

I
|
I

E

E

A

A

I

I

N

A

T

T

D

D

T

M

L

L

F

I

K

Q

L

L

E

E

K

E

E

E

G

G

F

L

F

N

V

V

A

V

P

P

C

C

A

A

N

R

A

A

V

T

W

K

T

L

A

T

M

M

H

N

R
|
R

E
|
E

R

G

L

I

R

R

E

R

A

L

I

A

A

A

-

E

S

E

T

L

G

Q

A

L

R

P

T

T

S

S

K

T

Y

Y

E

R

Y

F

A

A

T

D

D

S

L

E

E

S

S

L

F

F

K

R

A

E

A

A

C

V

K

A

K

D

I

I

G

G

F

Y

P

P

C

C

V
x
I

S

V

K
|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

S

Q

Y

T

V

F

L

I

K

R

S

S

S

A

K

E

D

Q

V

L

E

A

K

Q

A

A

F

W

K

K

E

Y

A

A

A

Q

K

Q

A

G

R

-

G

G

S

R

S

A

D

G

K

R

I

V

I

I

V

V

E
|
E

E

G

F
x
V

I
x
V

D

K

F
|
F

D

D

V

F

E
|
E

I

I

T

T

A

L

L

L

S

T

V

V

R

S

Y

A

L

V

N

D

G

G

K

-

G

-

K

-

P

-

E

-

S

V

K

H

F

F

V

C

R

A

P

P

L

V

G

G

H

H

Y

R

Q
|
Q

I

E

E

D

G

G

D

D

Y

Y

H

R

A

E

S

S

W

W

H

Q

P

P

W

-

T

-

D

-

A

-

T

-

D

-

K

Q

I

M

D

S

K

P

L

L

E

A

K

L

E

E

-

R

I

A

Y

Q

D

E

Y

I

A

A

G

R

R

K

I

V

M

V

D

L

K

A

L

L

G

G

Y

Y

G

G

L

L

F

F

A

G

H

V

E
|
E

M

L

F
|
F

V

V

D

-

T

-

K

C

N

G

G

D

K

E

V

V

Y

I

A

F

N

S

E
|
E

T

V

A

S

C

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

L

M

V

V

T

T

I

L

A

I

S

S

M

Q

P

D

F

L

G

-

Y

-

S

S

E

E

F

F

A

A

L

L

H

H

A

V

K

R

A

A

V

F

L

L

G

G

I

L

P

P

I

V

A

G

C

G

E

-

G

-

K

-

V

-

I

I

Q

R

P

Q

R

Y

S

G

T

P

A

A

S

S

H

A

V

V

I

I

L

L

S

P

H

Q

T

L

E

T

G

S

W

Q

Y

N

P

V

Q

T

F

F

-

D

K

N

V

V

D

Q

G

N

A

A

Y

V

A

G

P

A

D

D

T

L

N

Q

V

I

L

R

I

L

F

F

G

G

K
|
K

P

P

E

E

A

I

Y

D

E

G

E

S

R
|
R

L

L

G

G

V

V

V

A

L

L

A

A

T

T

A

A

G

E

T

S

V

V

E

V

D

D

A

A

K

I

K

E

K

R

A

A

Q

K

K

H

A

A

H

G

T

Q

V

V

K

K

V

V

S

Q

active site: E114 (= E119), K154 (= K158), S159 (= S163), G161 (= G165), E264 (= E279), E276 (= E293), D283 (= D300), T284 (= T301), R360 (= R382)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R118), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), E192 (= E197), V194 (≠ F199), V195 (≠ I200), F197 (= F202), E200 (= E205), Q222 (= Q232), E264 (= E279), F266 (= F281), E276 (= E293)
binding glycinamide ribonucleotide: G20 (= G25), E21 (= E26), L22 (= L27), E81 (= E86), I82 (= I87), S160 (= S164), D283 (= D300), K352 (= K374), R359 (= R381), R360 (= R382)
binding magnesium ion: E264 (= E279), E276 (= E293)

1eyzA Structure of escherichia coli purt-encoded glycinamide ribonucleotide transformylase complexed with mg and amppnp (see paper)
44% identity, 95% coverage: 8:410/426 of query aligns to 2:388/389 of 1eyzA

query
sites
1eyzA

V

L

L

L

G

G

T

T

P

A

Y

L

A

R

N

P

G

A

A

A

-

T

K

R

L

V

M

M

F

L

L

L

G

G

A

S

G

G

E

E

L

L

G

G

K

K

E

E

T

V

M

A

I

I

E

E

A

C

Q

Q

R

R

M

L

G

G

I

V

E

E

I

V

V

I

A

A

V

V

D

D

R

R

Y

Y

A

A

N

D

S

A

P

P

G

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

T

I

N

N

M

M

K

L

S

D

E

G

R

D

A

A

L

L

L

R

A

R

I

V

V

V

E

E

K

L

E

E

K

K

P

P

D

H

A

Y

I

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

N

A

T

T

D

D

T

M

L

L

F

I

K

Q

L

L

E

E

K

E

E

E

G

G

F

L

F

N

V

V

A

V

P

P

C

C

A

A

N

R

A

A

V

T

W

K

T

L

A

T

M

M

H

N

R
|
R

E
|
E

R

G

L

I

R

R

E

R

A

L

I

A

A

A

-

E

S

E

T

L

G

Q

A

L

R

P

T

T

S

S

K

T

Y

Y

E

R

Y

F

A

A

T

D

D

S

L

E

E

S

S

L

F

F

K

R

A

E

A

A

C

V

K

A

K

D

I

I

G

G

F

Y

P

P

C

C

V
x
I

S

V

K
|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

S
x
Q

Y

T

V

F

L

I

K

R

S

S

S

A

K

E

D

Q

V

L

E

A

K

Q

A

A

F

W

K

K

E

Y

A

A

A

Q

K

Q

A

G

R

-

G

G

S

R

S

A

D

G

K

R

I

V

I

I

V

V

E
|
E

E

G

F

V

I
x
V

D

K

F
|
F

D

D

V

F

E
|
E

I

I

T

T

A

L

L

L

S

T

V

V

R

S

Y

A

L

V

N

D

G

G

K

-

G

-

K

-

P

-

E

-

S

V

K

H

F

F

V

C

R

A

P

P

L

V

G

G

H

H

Y

R

Q

Q

I

E

E

D

G

G

D

D

Y

Y

H

R

A

E

S

S

W

W

H

Q

P

P

W

-

T

-

D

-

A

-

T

-

D

-

K

Q

I

M

D

S

K

P

L

L

E

A

K

L

E

E

-

R

I

A

Y

Q

D

E

Y

I

A

A

G

R

R

K

I

V

M

V

D

L

K

A

L

L

G

G

Y

Y

G

G

L

L

F

F

A

G

H

V

E
|
E

M

L

F
|
F

V

V

D

-

T

-

K

C

N

G

G

D

K

E

V

V

Y

I

A

F

N

S

E
|
E

T

V

A

S

C

P

R

R

P

P

H

H

D
|
D

T
|
T

G

G

L

M

V

V

T

T

I

L

A

I

S

S

M

Q

P

D

F

L

G

-

Y

-

S

S

E

E

F

F

A

A

L

L

H

H

A

V

K

R

A

A

V

F

L

L

G

G

I

L

P

P

I

V

A

G

C

G

E

-

G

-

K

-

V

-

I

I

Q

R

P

Q

R

Y

S

G

T

P

A

A

S

S

H

A

V

V

I

I

L

L

S

P

H

Q

T

L

E

T

G

S

W

Q

Y

N

P

V

Q

T

F

F

-

D

K

N

V

V

D

Q

G

N

A

A

Y

V

A

G

P

A

D

D

T

L

N

Q

V

I

L

R

I

L

F

F

G

G

K

K

P

P

E

E

A

I

Y

D

E

G

E

S

R

R

R
|
R

L

L

G

G

V

V

V

A

L

L

A

A

T

T

A

A

G

E

T

S

V

V

E

V

D

D

A

A

K

I

K

E

K

R

A

A

Q

K

K

H

A

A

H

G

T

Q

V

V

K

K

V

V

S

Q

active site: E114 (= E119), K154 (= K158), S159 (= S163), G161 (= G165), E264 (= E279), E276 (= E293), D283 (= D300), T284 (= T301), R360 (= R382)
binding phosphoaminophosphonic acid-adenylate ester: R113 (= R118), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (≠ S168), E192 (= E197), V195 (≠ I200), F197 (= F202), E200 (= E205), E264 (= E279), F266 (= F281), E276 (= E293)
binding magnesium ion: E264 (= E279), E276 (= E293)

P33221 Formate-dependent phosphoribosylglycinamide formyltransferase; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2; GART 2; GAR transformylase T; Non-folate glycinamide ribonucleotide transformylase; Phosphoribosylglycinamide formyltransferase 2; EC 6.3.1.21 from Escherichia coli (strain K12) (see 5 papers)
44% identity, 95% coverage: 8:410/426 of query aligns to 3:391/392 of P33221

query
sites
P33221

V

L

L

L

G

G

T

T

P

A

Y

L

A

R

N

P

G

A

A

A

-

T

K

R

L

V

M

M

F

L

L

L

G

G

A

S

G

G

E
|
E

L
|
L

G

G

K

K

E

E

T

V

M

A

I

I

E

E

A

C

Q

Q

R

R

M

L

G

G

I

V

E

E

I

V

V

I

A

A

V

V

D

D

R

R

Y

Y

A

A

N

D

S

A

P

P

G

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

T

I

N

N

M

M

K

L

S

D

E

G

R

D

A

A

L

L

L

R

A

R

I

V

V

V

E

E

K

L

E

E

K

K

P

P

D

H

A

Y

I

I

I

V

P

P

E
|
E

I

I

E

E

A

A

I

I

N

A

T

T

D

D

T

M

L

L

F

I

K

Q

L

L

E

E

K

E

E

E

G

G

F

L

F

N

V

V

A

V

P

P

C

C

A

A

N

R

A

A

V

T

W

K

T

L

A

T

M

M

H

N

R
|
R

E

E

R

G

L

I

R

R

E

R

A

L

I

A

A

A

-

E

S

E

T

L

G

Q

A

L

R

P

T

T

S

S

K

T

Y

Y

E

R

Y

F

A

A

T

D

D

S

L

E

E

S

S

L

F

F

K

R

A

E

A

A

C

V

K

A

K

D

I

I

G

G

F

Y

P

P

C

C

V

I

S

V

K
|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K
|
K

G
|
G

S
x
Q

Y

T

V

F

L

I

K

R

S

S

S

A

K

E

D

Q

V

L

E

A

K

Q

A

A

F

W

K
|
K

E

Y

A

A

A

Q

K

Q

-

G

A

G

R

R

G

A

S

G

S

A

D

G

K

R

I

V

I

I

V

V

E
|
E

E
x
G

F
x
V

I
x
V

D

K

F

F

D

D

V

F

E
|
E

I

I

T

T

A

L

L

L

S

T

V

V

R

S

Y

A

L

V

N

D

G

G

K

-

G

-

K

-

P

-

E

-

S

V

K

H

F

F

V

C

R

A

P

P

L

V

G

G

H

H

Y

R

Q

Q

I

E

E

D

G

G

D

D

Y

Y

H

R

A

E

S

S

W

W

H

Q

P

P

W

-

T

-

D

-

A

-

T

-

D

-

K

Q

I

M

D

S

K

P

L

L

E

A

K

L

E

E

-

R

I

A

Y

Q

D

E

Y

I

A

A

G

R

R

K

I

V

M

V

D

L

K

A

L

L

G

G

Y

Y

G

G

L

L

F

F

A

G

H

V

E
|
E

M

L

F

F

V

V

D

-

T

-

K

C

N

G

G

D

K

E

V

V

Y

I

A

F

N

S

E
|
E

T

V

A

S

C

P

R

R

P

P

H

H

D
|
D

T

T

G

G

L

M

V

V

T

T

I

L

A

I

S

S

M

Q

P

D

F

L

G

-

Y

-

S

S

E

E

F

F

A

A

L

L

H

H

A

V

K

R

A

A

V

F

L

L

G

G

I

L

P

P

I

V

A

G

C

G

E

-

G

-

K

-

V

-

I

I

Q

R

P

Q

R

Y

S

G

T

P

A

A

S

S

H

A

V

V

I

I

L

L

S

P

H

Q

T

L

E

T

G

S

W

Q

Y

N

P

V

Q

T

F

F

-

D

K

N

V

V

D

Q

G

N

A

A

Y

V

A

G

P

A

D

D

T

L

N

Q

V

I

L

R

I

L

F

F

G

G

K
|
K

P

P

E

E

A

I

Y

D

E

G

E

S

R
|
R

L

L

G

G

V

V

V

A

L

L

A

A

T

T

A

A

G

E

T

S

V

V

E

V

D

D

A

A

K

I

K

E

K

R

A

A

Q

K

K

H

A

A

H

G

T

Q

V

V

K

K

V

V

S

Q

EL 22:23 (= EL 26:27) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
E82 (= E86) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
R114 (= R118) binding ATP
K155 (= K158) binding ATP
SSGKGQ 160:165 (≠ SSGKGS 163:168) binding ATP
G162 (= G165) mutation to I: Strong decrease in the reaction rate for the conversion of formate to FGAR and in the affinity for formate. 3- and 2-fold decrease in the affinity for ATP and GAR, respectively.
K179 (= K182) modified: N6-acetyllysine
EGVV 195:198 (≠ EEFI 197:200) binding ATP
E203 (= E205) binding ATP
E267 (= E279) binding Mg(2+)
E279 (= E293) binding Mg(2+)
D286 (= D300) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
K355 (= K374) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide
RR 362:363 (= RR 381:382) binding N(1)-(5-phospho-beta-D-ribosyl)glycinamide

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

1kjjA Crystal structure of glycniamide ribonucleotide transformylase in complex with mg-atp-gamma-s (see paper)
44% identity, 95% coverage: 8:410/426 of query aligns to 2:385/386 of 1kjjA

query
sites
1kjjA

V

L

L

L

G

G

T

T

P

A

Y

L

A

R

N

P

G

A

A

A

-

T

K

R

L

V

M

M

F

L

L

L

G

G

A

S

G

G

E

E

L

L

G

G

K

K

E

E

T

V

M

A

I

I

E

E

A

C

Q

Q

R

R

M

L

G

G

I

V

E

E

I

V

V

I

A

A

V

V

D

D

R

R

Y

Y

A

A

N

D

S

A

P

P

G

A

M

M

Q

H

V

V

A

A

H

H

R

R

S

S

Y

H

V

V

T

I

N

N

M

M

K

L

S

D

E

G

R

D

A

A

L

L

L

R

A

R

I

V

V

V

E

E

K

L

E

E

K

K

P

P

D

H

A

Y

I

I

I

V

P

P

E

E

I

I

E

E

A

A

I

I

N

A

T

T

D

D

T

M

L

L

F

I

K

Q

L

L

E

E

K

E

E

E

G

G

F

L

F

N

V

V

A

V

P

P

C

C

A

A

N

R

A

A

V

T

W

K

T

L

A

T

M

M

H

N

R
|
R

E
|
E

R

G

L

I

R

R

E

R

A

L

I

A

A

A

-

E

S

E

T

L

G

Q

A

L

R

P

T

T

S

S

K

T

Y

Y

E

R

Y

F

A

A

T

D

D

S

L

E

E

S

S

L

F

F

K

R

A

E

A

A

C

V

K

A

K

D

I

I

G

G

F

Y

P

P

C

C

V
x
I

S

V

K
|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

S
x
Q

Y

T

V

F

L

I

K

R

S

S

S

A

K

E

D

Q

V

L

E

A

K

Q

A

A

F

W

K

K

E

Y

A

A

A

Q

K

Q

A

G

R

-

G

-

S

-

D

G

K

R

I

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I

I

V

V

E
|
E

E

G

F

V

I
x
V

D

K

F

F

D

D

V

F

E
|
E

I

I

T

T

A

L

L

L

S

T

V

V

R

S

Y

A

L

V

N

D

G

G

K

-

G

-

K

-

P

-

E

-

S

V

K

H

F

F

V

C

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A

P

P

L

V

G

G

H

H

Y

R

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|
Q

I

E

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D

G

G

D

D

Y

Y

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A

E

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S

W

W

H

Q

P

P

W

-

T

-

D

-

A

-

T

-

D

-

K

Q

I

M

D

S

K

P

L

L

E

A

K

L

E

E

-

R

I

A

Y

Q

D

E

Y

I

A

A

G

R

R

K

I

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M

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D

L

K

A

L

L

G

G

Y

Y

G

G

L

L

F

F

A

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H

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E
|
E

M

L

F
|
F

V

V

D

-

T

-

K

C

N

G

G

D

K

E

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I

A

F

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E
|
E

T

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A

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C

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R

R

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|
D

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|
T

G

G

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M

V

V

T

T

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M

Q

P

D

F

L

G

-

Y

-

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S

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E

F

F

A

A

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L

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H

A

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R

A

A

V

F

L

L

G

G

I

L

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P

I

V

A

G

C

G

E

-

G

-

K

-

V

-

I

I

Q

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Q

R

Y

S

G

T

P

A

A

S

S

H

A

V

V

I

I

L

L

S

P

H

Q

T

L

E

T

G

S

W

Q

Y

N

P

V

Q

T

F

F

-

D

K

N

V

V

D

Q

G

N

A

A

Y

V

A

G

P

A

D

D

T

L

N

Q

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I

L

R

I

L

F

F

G

G

K

K

P

P

E

E

A

I

Y

D

E

G

E

S

R

R

R
|
R

L

L

G

G

V

V

V

A

L

L

A

A

T

T

A

A

G

E

T

S

V

V

E

V

D

D

A

A

K

I

K

E

K

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A

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A

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Q

active site: E114 (= E119), K154 (= K158), S159 (= S163), G161 (= G165), E261 (= E279), E273 (= E293), D280 (= D300), T281 (= T301), R357 (= R382)
binding phosphothiophosphoric acid-adenylate ester: R113 (= R118), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (≠ S168), E189 (= E197), V192 (≠ I200), E197 (= E205), Q219 (= Q232), E261 (= E279), F263 (= F281), E273 (= E293)
binding magnesium ion: E261 (= E279), E273 (= E293)

1kj8A Crystal structure of purt-encoded glycinamide ribonucleotide transformylase in complex with mg-atp and gar (see paper)
44% identity, 95% coverage: 8:410/426 of query aligns to 2:385/386 of 1kj8A

query
sites
1kj8A

V

L

L

L

G

G

T

T

P

A

Y

L

A

R

N

P

G

A

A

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M

F

L

L

L

G

G

A

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G
|
G

E
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E

L
|
L

G

G

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K

E

E

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M

A

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I

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E

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Q

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E

E

I

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A

A

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D

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Y

A

A

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A

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P

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A

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M

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A

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Y

H

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I

N

N

M

M

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R

D

A

A

L

L

L

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A

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K

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I

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E

I
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I

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E

A

A

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I

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M

L

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Q

L

L

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K

E

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E

G

G

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F

N

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A

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P

C

C

A

A

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R

A

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E

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R

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I

A

A

A

-

E

S

E

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A

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S

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T

Y

Y

E

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Y

F

A

A

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D

D

S

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E

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S

L

F

F

K

R

A

E

A

A

C

V

K

A

K

D

I

I

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G

F

Y

P

P

C

C

V
x
I

S

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|
K

P

P

I

V

M

M

S

S

S
|
S

G
|
G

K

K

G

G

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x
Q

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S

S

A

K

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D

Q

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E

A

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A

A

F

W

K

K

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Y

A

A

A

Q

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Q

A

G

R

-

G

-

S

-

D

G

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I

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I

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E
|
E

E

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F

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x
V

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F
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F

D

D

V

F

E
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E

I

I

T

T

A

L

L

L

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T

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V

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S

Y

A

L

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D

G

G

K

-

G

-

K

-

P

-

E

-

S

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A

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P

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V

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G

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Q

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G

D

D

Y

Y

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A

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S

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W

H

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P

W

-

T

-

D

-

A

-

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-

K

Q

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P

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L

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A

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-

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Y

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A

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L

K

A

L

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G

G

Y

Y

G

G

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L

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F

A

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E
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E

M

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F
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F

V

V

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-

K

C

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G

G

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K

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A

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E
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E

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D
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D

T
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T

G

G

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M

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V

T

T

I

L

A

I

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S

M

Q

P

D

F

L

G

-

Y

-

S

S

E

E

F

F

A

A

L

L

H

H

A

V

K

R

A

A

V

F

L

L

G

G

I

L

P

P

I

V

A

G

C

G

E

-

G

-

K

-

V

-

I

I

Q

R

P

Q

R

Y

S

G

T

P

A

A

S

S

H

A

V

V

I

I

L

L

S

P

H

Q

T

L

E

T

G

S

W

Q

Y

N

P

V

Q

T

F

F

-

D

K

N

V

V

D

Q

G

N

A

A

Y

V

A

G

P

A

D

D

T

L

N

Q

V

I

L

R

I

L

F

F

G

G

K
|
K

P

P

E

E

A

I

Y

D

E

G

E

S

R
|
R

L

L

G

G

V

V

V

A

L

L

A

A

T

T

A

A

G

E

T

S

V

V

E

V

D

D

A

A

K

I

K

E

K

R

A

A

Q

K

K

H

A

A

H

G

T

Q

V

V

K

K

V

V

S

Q

active site: E114 (= E119), K154 (= K158), S159 (= S163), G161 (= G165), E261 (= E279), E273 (= E293), D280 (= D300), T281 (= T301), R357 (= R382)
binding adenosine-5'-triphosphate: R113 (= R118), I152 (≠ V156), K154 (= K158), S159 (= S163), S160 (= S164), G161 (= G165), Q164 (≠ S168), E189 (= E197), V192 (≠ I200), F194 (= F202), E197 (= E205), Q219 (= Q232), G222 (= G235), E261 (= E279), F263 (= F281), E273 (= E293)
binding glycinamide ribonucleotide: G20 (= G25), E21 (= E26), L22 (= L27), E81 (= E86), I82 (= I87), S160 (= S164), D280 (= D300), K349 (= K374), R356 (= R381)
binding magnesium ion: E261 (= E279), E273 (= E293)

1kjqA Crystal structure of glycinamide ribonucleotide transformylase in complex with mg-adp (see paper)
43% identity, 95% coverage: 8:410/426 of query aligns to 2:387/388 of 1kjqA

query
sites
1kjqA

V

L

L

L

G

G

T

T

P

A

Y

L

A

R

N

P

G

A

A

A

-

T

K

R

L

V

M

M

F

L

L

L

G

G

A

S

G

G

E

E

L

L

G

G

K

K

E

E

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E

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Q

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M

L

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G

I

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E

E

I

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A

A

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D

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R

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Y

A

A

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A

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P

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A

M

M

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H

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A

A

H

H

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Y

H

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T

I

N

N

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M

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D

E

G

R

D

A

A

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L

L

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A

R

I

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E

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L

E

E

K

K

P

P

D

H

A

Y

I

I

I

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P

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E

I

I

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E

A

A

I

I

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A

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M

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Q

L

L

E

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K

E

E

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G

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F

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A

A

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A

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|
R

E
|
E

R

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I

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E

R

A

L

I

A

A

A

-

E

S

E

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A

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Y

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R

Y

F

A

A

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D

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S

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K

R

A

E

A

A

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K

A

K

D

I

I

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G

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Y

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P

C

C

V
x
I

S

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K
|
K

P

P

I

V

M

M

S

S

S

-

G

-

K

K

G

G

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Y

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S

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A

K

E

D

Q

V

L

E

A

K

Q

A

A

F

W

K

K

E

Y

A

A

A

Q

K

Q

-

G

A

G

R

R

G

A

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G

S

A

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G

K

R

I

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I

I

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V

E
|
E

E

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x
V

I
x
V

D

K

F
|
F

D

D

V

F

E
|
E

I

I

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T

A

L

L

L

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T

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S

Y

A

L

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-

G

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-

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K

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A

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|
Q

I

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D

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G

D

D

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Y

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A

E

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S

W

W

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P

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Y

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A

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L

K

A

L

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Y

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E

M

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F

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T

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G

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F

A

A

L

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A

A

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F

L

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P

I

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A

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C

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G

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K

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Q

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A

A

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G

S

W

Q

Y

N

P

V

Q

T

F

F

-

D

K

N

V

V

D

Q

G

N

A

A

Y

V

A

G

P

A

D

D

T

L

N

Q

V

I

L

R

I

L

F

F

G

G

K

K

P

P

E

E

A

I

Y

D

E

G

E

S

R

R

R
|
R

L

L

G

G

V

V

V

A

L

L

A

A

T

T

A

A

G

E

T

S

V

V

E

V

D

D

A

A

K

I

K

E

K

R

A

A

Q

K

K

H

A

A

H

G

T

Q

V

V

K

K

V

V

S

Q

active site: E114 (= E119), K154 (= K158), E263 (= E279), E275 (= E293), D282 (= D300), T283 (= T301), R359 (= R382)
binding adenosine-5'-diphosphate: R113 (= R118), I152 (≠ V156), K154 (= K158), E191 (= E197), V193 (≠ F199), V194 (≠ I200), F196 (= F202), E199 (= E205), Q221 (= Q232), F265 (= F281), E275 (= E293)
binding magnesium ion: E263 (= E279), E275 (= E293)

3v4sA Crystal structure of adp-atp complex of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 88% coverage: 25:401/426 of query aligns to 16:366/380 of 3v4sA

query
sites
3v4sA

G

G

E

Q

L

L

G

G

K

R

E

M

T

M

M

A

I

L

E

A

A

A

Q

K

R

E

M

M

G

G

I

Y

E

K

I

I

V

A

A

V

V

L

D

D

R

P

Y

T

A

K

N

N

S

S

P

P

G

C

M

A

Q

Q

V

V

A

A

H

D

R

I

S

E

Y

I

V

V

T

A

N

S

M

Y

K

D

S

D

E

L

R

K

A

A

L

I

L

Q

A

H

I

L

V

A

E

E

K

I

E

S

K

-

P

-

D

D

A

V

I

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

N

D

T

Y

D

R

T

C

L

L

F

Q

K

W

L

L

E

E

K

K

E

H

G

A

F

Y

F

L

V

-

A

-

P

P

C

Q

A

G

N

S

A

Q

V

L

W

L

T

S

-

K

A

T

M

Q

H

N

R
|
R

E

F

R

T

L

E

R

K

E

N

A

A

I

I

A

E

S

K

T

A

G

G

A

L

R

P

T

V

S

A

K

T

Y

Y

E

R

Y

L

A

V

T

Q

D

N

L

Q

E

E

S

Q

F

L

K

T

A

E

A

A

C

I

K

A

K

E

I

L

G

S

F

Y

P

P

C

S

V

V

S

L

K
|
K

P

T

I

T

M

T

S

G

-
x
Y

S

D

G
|
G

K

K

G

G

S
x
Q

Y

V

V

V

L

L

K

R

S

S

S

E

K

A

D

D

V

V

E

D

K

E

A

A

F

R

K

K

E

L

A

A

A

N

K

A

A

A

R

-

G

-

S

-

D

-

K

E

I

C

I

I

V

L

E

E

E

K

F
x
W

I
x
V

D

P

F

F

D

E

V

K

E
|
E

I

V

T

S

A

V

L

I

S

V

V

I

R

R

Y

S

L

V

N

S

G

G

K

E

G

T

K

K

P

-

E

-

S

-

K

-

F

-

V

V

R

F

P

P

L

V

G

A

-

E

H

N

Y

I

Q

H

I

V

E

N

G
x
N

D

I

Y

L

H

H

A

E

S

S

W

I

H

V

P

P

W

-

T

-

D

-

A

-

T

-

D

A

K

R

K

I

I

T

D

E

K

E

L

L

E

S

K

Q

E

K

I

A

Y

I

D

A

Y

Y

A

A

G

K

R

V

I

L

M

A

D

D

K

E

L

L

G

E

G

L

Y

V

G

G

L

T

F

L

A

A

H

V

E

E

M

M

F
|
F

V

A

D

-

T

T

K

A

N

D

G

G

K

E

V

I

Y

Y

A

I

N
|
N

E
|
E

T

L

A

A

C

P

R
|
R

P

P

H
|
H

D
x
N

T

S

G

G

L

H

V

Y

T

T

I

Q

A

D

S

A

M

C

P

E

F

-

G

-

Y

T

S

S

E

Q

F

F

A

G

L

Q

H

H

A

I

K

R

A

A

V

I

L

C

G

N

I

L

P

P

I

L

A

G

C

-

E

E

G

T

K

N

V

L

I

L

Q

K

P

P

R

-

S

-

T

V

A

V

A

M

S

V

H

N

V

I

I

L

L

G

S

E

H

H

T

I

E

E

G

G

W

V

Y

L

P

R

Q

Q

F

V

-

N

K

R

V

L

D

T

G

G

A

C

Y

Y

A

-

P

-

D

-

T

-

N

-

V

L

L

H

I

L

F

Y

G

G

K

K

P

E

E

E

A

A

Y

K

E

A

E

Q

R

R

R

K

L

M

G

G

V

H

V

V

L

N

A

I

T

L

A

N

G

D

T

N

V

I

E

E

D

V

A

A

K

L

K

E

K

K

A

A

Q

K

binding adenosine-5'-diphosphate: R106 (= R118), K146 (= K158), Y152 (vs. gap), G154 (= G165), Q157 (≠ S168), W183 (≠ F199), V184 (≠ I200), E189 (= E205), N215 (≠ G235), F256 (= F281), N266 (= N292), E267 (= E293)
binding carbonate ion: R271 (= R297), H273 (= H299), N274 (≠ D300)

3q2oB Crystal structure of purk: n5-carboxyaminoimidazole ribonucleotide synthetase (see paper)
28% identity, 88% coverage: 25:401/426 of query aligns to 17:367/377 of 3q2oB

query
sites
3q2oB

G

G

E

Q

L

L

G

G

K

R

E

M

T

M

M

A

I

L

E

A

A

A

Q

K

R

E

M

M

G

G

I

Y

E

K

I

I

V

A

A

V

V

L

D

D

R

P

Y

T

A

K

N

N

S

S

P

P

G

C

M

A

Q

Q

V

V

A

A

H

D

R

I

S

E

Y

I

V

V

T

A

N

S

M

Y

K

D

S

D

E

L

R

K

A

A

L

I

L

Q

A

H

I

L

V

A

E

E

K

I

E

S

K

-

P

-

D

D

A

V

I

V

I

T

P

Y

E

E

I

F

E

E

A

N

I

I

N

D

T

Y

D

R

T

C

L

L

F

Q

K

W

L

L

E

E

K

K

E

H

G

A

F

Y

F

L

V

-

A

-

P

P

C

Q

A

G

N

S

A

Q

V

L

W

L

T

S

-

K

A
x
T

M

Q

H

N

R

R

E

F

R

T

L
x
E

R

K

E

N

A

A

I

I

A

E

S

K

T

A

G

G

A

L

R

P

T

V

S

A

K

T

Y

Y

E

R

Y

L

A

V

T

Q

D

N

L

Q

E

E

S

Q

F

L

K

T

A

E

A

A

C

I

K

A

K

E

I

L

G

S

F

Y

P

P

C

S

V

V

S

L

K

K

P

T

I

T

M

T

S

G

-

Y

S

D

G

G

K

K

G

G

S

Q

Y

V

V

V

L

L

K

R

S

S

S

E

K

A

D

D

V

V

E

D

K

E

A

A

F

R

K

K

E

L

A

A

A

N

K

A

A

A

R

-

G

-

S

-

D

-

K

E

I

C

I

I

V

L

E

E

E

K

F

W

I

V

D

P

F

F

D

E

V

K

E

E

I

V

T

S

A

V

L

I

S

V

V

I

R

R

Y

S

L

V

N

S

G

G

K

E

G

T

K

K

P

-

E

-

S

-

K

-

F

-

V

V

R

F

P

P

L

V

G

A

-

E

H

N

Y

I

Q

H

I

V

E

N

G

N

D

I

Y

L

H

H

A

E

S

S

W

I

H

V

P

P

W

-

T

-

D

-

A

-

T

-

D

A

K

R

K

I

I

T

D

E

K

E

L

L

E

S

K

Q

E

K

I

A

Y

I

D

A

Y

Y

A

A

G

K

R

V

I

L

M

A

D

D

K

E

L

L

G

E

G

L

Y

V

G

G

L

T

F

L

A

A

H

V

E

E

M

M

F

F

V

A

D

-

T

T

K

A

N

D

G

G

K

E

V

I

Y

Y

A

I

N

N

E

E

T
x
L

A

A

C

P

R

R

P

P

H

H

D

N

T

S

G

G

L

H

V

Y

T

T

I

Q

A

D

S

A

M

C

P

E

F

-

G

-

Y

T

S

S

E

Q

F

F

A

G

L

Q

H

H

A

I

K

R

A

A

V

I

L

C