SitesBLAST
Comparing WP_012042026.1 NCBI__GCF_000015165.1:WP_012042026.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
5zaiC Crystal structure of 3-hydroxypropionyl-coa dehydratase from metallosphaera sedula (see paper)
37% identity, 92% coverage: 16:256/261 of query aligns to 17:252/259 of 5zaiC
- active site: A65 (= A65), F70 (= F70), S82 (≠ V86), R86 (≠ Q90), G110 (= G114), E113 (= E117), P132 (≠ S136), E133 (= E137), I138 (≠ M142), P140 (= P144), G141 (≠ T145), A226 (≠ Q230), F236 (≠ V240)
- binding coenzyme a: K24 (≠ R23), L25 (= L24), A63 (= A63), G64 (= G64), A65 (= A65), D66 (= D66), I67 (= I67), P132 (≠ S136), R166 (≠ A170), F248 (≠ W252), K251 (≠ R255)
2hw5C The crystal structure of human enoyl-coenzyme a (coa) hydratase short chain 1, echs1
38% identity, 95% coverage: 10:256/261 of query aligns to 14:253/260 of 2hw5C
- active site: A68 (= A65), M73 (≠ F70), S83 (= S79), L87 (≠ V86), G111 (= G114), E114 (= E117), P133 (≠ S136), E134 (= E137), T139 (≠ M142), P141 (= P144), G142 (≠ T145), K227 (≠ Q230), F237 (≠ V240)
- binding crotonyl coenzyme a: K26 (≠ E22), A27 (≠ R23), L28 (= L24), A30 (= A26), K62 (≠ R59), I70 (= I67), F109 (= F112)
1dubA 2-enoyl-coa hydratase, data collected at 100 k, ph 6.5 (see paper)
36% identity, 93% coverage: 13:256/261 of query aligns to 17:253/260 of 1dubA
- active site: A68 (= A65), M73 (≠ F70), S83 (≠ D76), L87 (≠ V80), G111 (= G114), E114 (= E117), P133 (≠ S136), E134 (= E137), T139 (≠ M142), P141 (= P144), G142 (≠ T145), K227 (≠ Q230), F237 (≠ V240)
- binding acetoacetyl-coenzyme a: K26 (≠ E22), A27 (≠ R23), L28 (= L24), A30 (= A26), A66 (= A63), A68 (= A65), D69 (= D66), I70 (= I67), Y107 (≠ L110), G110 (= G113), G111 (= G114), E114 (= E117), P133 (≠ S136), E134 (= E137), L137 (= L140), G142 (≠ T145), F233 (= F236), F249 (≠ W252)
1mj3A Crystal structure analysis of rat enoyl-coa hydratase in complex with hexadienoyl-coa (see paper)
36% identity, 93% coverage: 13:256/261 of query aligns to 17:251/258 of 1mj3A
- active site: A68 (= A65), M73 (≠ F70), S83 (≠ R88), L85 (≠ Q90), G109 (= G114), E112 (= E117), P131 (≠ S136), E132 (= E137), T137 (≠ M142), P139 (= P144), G140 (≠ T145), K225 (≠ Q230), F235 (≠ V240)
- binding hexanoyl-coenzyme a: K26 (≠ E22), A27 (≠ R23), L28 (= L24), A30 (= A26), A66 (= A63), G67 (= G64), A68 (= A65), D69 (= D66), I70 (= I67), G109 (= G114), P131 (≠ S136), E132 (= E137), L135 (= L140), G140 (≠ T145)
1ey3A Structure of enoyl-coa hydratase complexed with the substrate dac-coa (see paper)
36% identity, 93% coverage: 13:256/261 of query aligns to 15:251/258 of 1ey3A
- active site: A66 (= A65), M71 (≠ F70), S81 (≠ D76), L85 (≠ V80), G109 (= G114), E112 (= E117), P131 (≠ S136), E132 (= E137), T137 (≠ M142), P139 (= P144), G140 (≠ T145), K225 (≠ Q230), F235 (≠ V240)
- binding 4-(n,n-dimethylamino)cinnamoyl-coa: K24 (≠ E22), L26 (= L24), A28 (= A26), A64 (= A63), G65 (= G64), A66 (= A65), D67 (= D66), I68 (= I67), L85 (≠ V80), W88 (≠ R83), G109 (= G114), P131 (≠ S136), L135 (= L140), G140 (≠ T145)
P14604 Enoyl-CoA hydratase, mitochondrial; mECH; mECH1; Enoyl-CoA hydratase 1; ECHS1; Short-chain enoyl-CoA hydratase; SCEH; EC 4.2.1.17; EC 5.3.3.8 from Rattus norvegicus (Rat) (see 3 papers)
36% identity, 93% coverage: 13:256/261 of query aligns to 47:283/290 of P14604
- E144 (= E117) mutation to D: Reduces activity 50-fold.; mutation to Q: Reduces activity 3300-fold.
- E164 (= E137) mutation to D: Reduces activity 1250-fold.; mutation to Q: Reduces activity 330000-fold.
Sites not aligning to the query:
- 1:29 modified: transit peptide, Mitochondrion
2dubA Enoyl-coa hydratase complexed with octanoyl-coa (see paper)
36% identity, 93% coverage: 13:256/261 of query aligns to 16:247/254 of 2dubA
- active site: A67 (= A65), M72 (≠ F70), S82 (≠ R87), G105 (= G114), E108 (= E117), P127 (≠ S136), E128 (= E137), T133 (≠ M142), P135 (= P144), G136 (≠ T145), K221 (≠ Q230), F231 (≠ V240)
- binding octanoyl-coenzyme a: K25 (≠ E22), A26 (≠ R23), L27 (= L24), A29 (= A26), A65 (= A63), A67 (= A65), D68 (= D66), I69 (= I67), K70 (≠ H68), G105 (= G114), E108 (= E117), P127 (≠ S136), E128 (= E137), G136 (≠ T145), A137 (≠ F146)
3q0jC Crystal structure of the mycobacterium tuberculosis crotonase in complex with the inhibitor acetoacetylcoa
36% identity, 97% coverage: 3:256/261 of query aligns to 4:250/255 of 3q0jC
- active site: A65 (= A65), M70 (≠ F70), T80 (≠ S79), F84 (≠ V86), G108 (= G114), E111 (= E117), P130 (≠ S136), E131 (= E137), V136 (≠ M142), P138 (= P144), G139 (≠ T145), L224 (≠ Q230), F234 (≠ V240)
- binding acetoacetyl-coenzyme a: Q23 (≠ E22), A24 (≠ R23), L25 (= L24), A27 (= A26), A63 (= A63), G64 (= G64), A65 (= A65), D66 (= D66), I67 (= I67), K68 (≠ H68), M70 (≠ F70), F84 (≠ V86), G107 (= G113), G108 (= G114), E111 (= E117), P130 (≠ S136), E131 (= E137), P138 (= P144), G139 (≠ T145), M140 (≠ F146)
3q0gC Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
36% identity, 97% coverage: 3:256/261 of query aligns to 4:250/255 of 3q0gC
- active site: A65 (= A65), M70 (≠ F70), T80 (≠ S79), F84 (≠ V86), G108 (= G114), E111 (= E117), P130 (≠ S136), E131 (= E137), V136 (≠ M142), P138 (= P144), G139 (≠ T145), L224 (≠ Q230), F234 (≠ V240)
- binding coenzyme a: L25 (= L24), A63 (= A63), I67 (= I67), K68 (≠ H68), Y104 (≠ L110), P130 (≠ S136), E131 (= E137), L134 (= L140)
3h81A Crystal structure of enoyl-coa hydratase from mycobacterium tuberculosis (see paper)
36% identity, 97% coverage: 3:256/261 of query aligns to 3:249/256 of 3h81A
- active site: A64 (= A65), M69 (≠ F70), T79 (≠ S79), F83 (≠ V86), G107 (= G114), E110 (= E117), P129 (≠ S136), E130 (= E137), V135 (≠ M142), P137 (= P144), G138 (≠ T145), L223 (≠ Q230), F233 (≠ V240)
- binding calcium ion: F233 (≠ V240), Q238 (≠ L245)
3q0gD Crystal structure of the mycobacterium tuberculosis crotonase bound to a reaction intermediate derived from crotonyl coa
35% identity, 97% coverage: 3:256/261 of query aligns to 3:245/250 of 3q0gD
- active site: A64 (= A65), M69 (≠ F70), T75 (≠ V86), F79 (≠ Q90), G103 (= G114), E106 (= E117), P125 (≠ S136), E126 (= E137), V131 (≠ M142), P133 (= P144), G134 (≠ T145), L219 (≠ Q230), F229 (≠ V240)
- binding Butyryl Coenzyme A: F225 (= F236), F241 (≠ W252)
6slbAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
39% identity, 97% coverage: 5:257/261 of query aligns to 5:251/257 of 6slbAAA
- active site: Q64 (≠ A65), F69 (= F70), L80 (≠ V86), N84 (≠ Q90), A108 (≠ G114), S111 (≠ E117), A130 (≠ S136), F131 (≠ E137), L136 (≠ M142), P138 (= P144), D139 (≠ T145), A224 (≠ Q230), G234 (≠ V240)
- binding (~{E})-6-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethylsulfanyl]-6-oxidanylidene-hex-3-enoic acid: R58 (= R59), A62 (= A63), Q64 (≠ A65), D65 (= D66), L66 (≠ I67), Y76 (≠ L82), A108 (≠ G114), F131 (≠ E137), D139 (≠ T145)
6slaAAA Enoyl-CoA hydratase/carnithine racemase (see paper)
38% identity, 97% coverage: 5:257/261 of query aligns to 2:239/245 of 6slaAAA
- active site: Q61 (≠ A65), L68 (≠ V86), N72 (≠ Q90), A96 (≠ G114), S99 (≠ E117), A118 (≠ S136), F119 (≠ E137), L124 (≠ M142), P126 (= P144), N127 (≠ T145), A212 (≠ Q230), G222 (≠ V240)
- binding ~{S}-[2-[3-[[(2~{R})-4-[[[(2~{R},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-4-oxidanyl-3-phosphonooxy-oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxy-3,3-dimethyl-2-oxidanyl-butanoyl]amino]propanoylamino]ethyl] 2-(2,5-dihydrooxepin-7-yl)ethanethioate: L21 (= L24), A59 (= A63), Q61 (≠ A65), D62 (= D66), L63 (≠ I67), L68 (≠ V86), Y71 (≠ G89), A94 (≠ F112), G95 (= G113), A96 (≠ G114), F119 (≠ E137), I122 (≠ L140), L124 (≠ M142), N127 (≠ T145), F234 (≠ W252), K237 (≠ R255)
O69762 Hydroxycinnamoyl-CoA hydratase-lyase; HCHL; P-hydroxycinnamoyl CoA hydratase/lyase; Trans-feruloyl-CoA hydratase/vanillin synthase; EC 4.1.2.61 from Pseudomonas fluorescens (see 2 papers)
37% identity, 84% coverage: 4:222/261 of query aligns to 10:224/276 of O69762
- K29 (≠ R23) binding acetyl-CoA
- A68 (= A63) binding acetyl-CoA
- M70 (≠ A65) binding acetyl-CoA
- L72 (≠ I67) binding acetyl-CoA
- Y75 (≠ F70) binding vanillin
- G120 (= G114) binding acetyl-CoA
- S123 (≠ E117) mutation to A: Reduced kcat compared to wild-type but not markerdly.
- S142 (= S136) binding acetyl-CoA
- E143 (= E137) mutation to A: Abolishes catalytic activity.
- W146 (≠ L140) binding acetyl-CoA
- G151 (≠ T145) binding vanillin
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 239 binding vanillin; Y→F: Increased KM for feruloyl-CoA but retains a significant amount of catalytic activity with a kcat 10 times less than that of the wild-type.
2vssB Wild-type hydroxycinnamoyl-coa hydratase lyase in complex with acetyl- coa and vanillin (see paper)
37% identity, 84% coverage: 4:222/261 of query aligns to 7:221/247 of 2vssB
- active site: M67 (≠ A65), Y72 (≠ F70), D77 (≠ G75), R89 (= R88), Q93 (= Q90), G117 (= G114), S120 (≠ E117), S139 (= S136), E140 (= E137), I145 (≠ M142), P147 (= P144), G148 (≠ T145)
- binding acetyl coenzyme *a: E25 (= E22), K26 (≠ R23), R27 (≠ L24), A29 (= A26), A65 (= A63), M67 (≠ A65), D68 (= D66), W113 (≠ L110), F115 (= F112), G117 (= G114), S139 (= S136), E140 (= E137)
Sites not aligning to the query:
2vssD Wild-type hydroxycinnamoyl-coa hydratase lyase in complex with acetyl- coa and vanillin (see paper)
37% identity, 84% coverage: 4:222/261 of query aligns to 8:222/246 of 2vssD
- active site: M68 (≠ A65), Y73 (≠ F70), D78 (≠ G75), R90 (= R88), Q94 (= Q90), G118 (= G114), S121 (≠ E117), S140 (= S136), E141 (= E137), I146 (≠ M142), P148 (= P144), G149 (≠ T145)
- binding acetyl coenzyme *a: E26 (= E22), K27 (≠ R23), R28 (≠ L24), A30 (= A26), A66 (= A63), M68 (≠ A65), D69 (= D66), L70 (≠ I67), F74 (≠ A71), W114 (≠ L110), F116 (= F112), S140 (= S136)
- binding 4-hydroxy-3-methoxybenzaldehyde: M68 (≠ A65), Y73 (≠ F70), F74 (≠ A71), Q96 (≠ M92), E141 (= E137), G149 (≠ T145), N150 (≠ F146)
Sites not aligning to the query:
5jbxB Crystal structure of liuc in complex with coenzyme a and malonic acid (see paper)
36% identity, 93% coverage: 13:256/261 of query aligns to 15:254/261 of 5jbxB
- active site: A67 (= A65), R72 (≠ A71), L84 (≠ R83), R88 (= R87), G112 (= G114), E115 (= E117), T134 (≠ S136), E135 (= E137), I140 (≠ M142), P142 (= P144), G143 (≠ T145), A228 (≠ Q230), L238 (≠ V240)
- binding coenzyme a: S24 (≠ E22), R25 (= R23), R26 (≠ L24), A28 (= A26), A65 (= A63), D68 (= D66), L69 (≠ I67), K70 (≠ H68), L110 (≠ F112), G111 (= G113), T134 (≠ S136), E135 (= E137), L138 (= L140), R168 (≠ A170)
6l3pA Crystal strcuture of feruloyl-coa hydratase lyase(fchl) complexed with coa
35% identity, 84% coverage: 4:221/261 of query aligns to 9:217/244 of 6l3pA
- active site: M69 (≠ A65), Y74 (≠ F70), R86 (= R88), Q90 (= Q90), G114 (= G114), S117 (≠ E117), S136 (= S136), E137 (= E137), I142 (≠ M142), P144 (= P144), G145 (≠ T145)
- binding coenzyme a: K28 (≠ R23), R29 (≠ L24), A31 (= A26), A67 (= A63), M69 (≠ A65), D70 (= D66), L71 (≠ I67), G113 (= G113)
Sites not aligning to the query:
3p85A Crystal structure enoyl-coa hydratase from mycobacterium avium (see paper)
37% identity, 88% coverage: 3:232/261 of query aligns to 1:210/224 of 3p85A
- active site: L62 (≠ A65), L67 (≠ F70), P68 (≠ Q90), G92 (= G114), E95 (= E117), T114 (≠ S136), H115 (≠ E137), L120 (≠ M142), P122 (= P144), T123 (= T145), W208 (≠ Q230)
- binding calcium ion: D43 (= D45), D45 (≠ G47)
Sites not aligning to the query:
Q4WF54 Mevalonyl-coenzyme A hydratase sidH; Siderophore biosynthesis protein H; EC 4.2.1.- from Aspergillus fumigatus (strain ATCC MYA-4609 / CBS 101355 / FGSC A1100 / Af293) (Neosartorya fumigata) (see paper)
34% identity, 95% coverage: 10:256/261 of query aligns to 18:261/270 of Q4WF54
Sites not aligning to the query:
- 268:270 PTS1-type peroxisomal targeting signal
Query Sequence
>WP_012042026.1 NCBI__GCF_000015165.1:WP_012042026.1
MTDTVLLDITDRIALLTLNRPERLNALSYRLIDALMAMLDRIETDAGVRAIILTGAGDRA
FSAGADIHEFAASVGDGRSVALRDFVRRGQAMTSRLEAFPKPIIAAVNGLAFGGGCEITE
AVHLAVASTRASFAKSEIRLGMPPTFGGTQRLPRLVGRKRGLELLLTGDAIPAQRAAEIG
LVNAVVPHDQMLPAARELAKRIIRHGPEAVTSVITAATRGLNMAIGEGLQVEGEQFGRLV
GGRELEHGLAAWRERRPRASL
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory