SitesBLAST

4lnkA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of gs-glutamate-amppcp complex (see paper)
31% identity, 99% coverage: 5:430/432 of query aligns to 8:440/443 of 4lnkA

query
sites
4lnkA

L

I

H

E

A

K

V

L

A

V

C

K

E

E

R

E

G

N

I

V

K

K

Y

Y

F

I

L

R

I

L

S

Q

Y

F

T

T

D

D

L

I

F

L

G

G

V

T

Q

I

R

K

A

N

K

V

L

E

V

I

P

P

A

V

A

S

A

Q

I

L

D

G

G
x
K

M

A

V

L

K

D

T

N

G

K

A

V

G
x
M

F

F

A
x
D

G

G

F

S

A

S

T

I

W

E

L

G

D

F

M

V

T

R

P

I

A

E

D

E

P

S

D

D

V

M

F

Y

A

L

M

Y

P

P

D

D

P

L

A

N

S

T

L

F

I

V

Q

I

L

F

P

P

W

W

K

T

P

A

E

E

V

K

G

G

W

K

L

V

A

A

-

R

-

F

-

I

A

C

D

D

P

I

W

Y

M

N

-

P

N

D

G

G

K

T

P

P

V

F

A

E

H

G

A

D

P

P

R

R

N

N

L

N

L

L

K

K

R

R

M

I

V

L

A

K

D

E

A

M

A

E

A

D

Q

L

G

G

Y

F

-

S

E

D

L

F

K

N

S

L

G

G

V

P

E
|
E

C

P

E
|
E

F

F

L

L

I

F

T

K

A

L

D

D

G

E

S

K

A

G

P

E

S

P

D

T

V

L

A

E

D

L

K

N

Q

D

S

K

K

G

P

G

C

Y

Y

F

D

D

Q

L

Q

A

A

P

L

T

M

D

R

L

R

G

Y

E

E

N

V

C

I

R

A

R

E

D

I

I

C

V

D

L

A

E

M

L

L

E

A

E

L

M

G

G

W

F

K

E

P

I

Y

E

Q

A

N

S

D

H

H

H

E
|
E

D
x
V

A

A

N

P

G

G

Q

Q

F

H

E
|
E

M

I

N

D

W
x
F

E

K

F
x
Y

D

A

T

G

A

A

L

V

T

R

T

S

A

C

D

D

R

D

H

I

A

Q

F

T

F

F

K

K

Y

L

M

V

V

V

R

K

S

T

I

I

A

A

E

R

K

K

H

H

G

G

L

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

V

F

D

G

L

V

T
x
N

G
|
G

N

S

G
|
G

C

M

H
|
H

A

C

H
x
N

V

L

S
|
S

V

L

W

F

K

K

D

N

G

G

R

V

N

N

L

A

F

F

E

F

D

D

E

E

A

N

G

A

E

D

L

L

G

Q

V

L

S

S

E

E

L

T

G

A

Y

K

Q

H

F

F

L

I

G

A

G

G

I

I

M

V

H

K

S

H

A

A

D

T

A

S

L

F

A

T

A

A

L

V

F

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

I

L

N

V

A

P

A

G

R

Y

T
x
E

T

-

S

-

G

-

A

-

T

-

W

-

A

A

P

P

N

C

S

Y

V

V

T

A

Y

W

S

S

G

A

N

Q

N

N

R
|
R

T

S

H

P

L

L

I

I

R

R

I

I

P

P

E

A

-
x
S

-

R

-

G

-

I

A
x
S

G

T

R
|
R

F

V

E
|
E

V

V

R
|
R

L

S

A

V

D

D

G

P

A

A

N

N

P

P

Y

Y

L

L

L

A

Q

L

A

S

G

V

L

L

A

A

G

G

L

L

D

D

G

G

I

I

A

K

N

N

D

K

R

L

D

E

P

A

G

P

P

A

R

P

L

I

D

D

I

R

N

N

M

I

Y

Y

T

V

D

M

G

S

H

K

A

E

V

E

K

R

T

M

G

E

K

N

-

G

-

I

-

V

K

D

L

L

P

P

L

A

N

T

L

L

L

A

D

E

A

A

I

L

R

E

A

E

L

F

Q

K

A

S

S

N

P

E

V

V

M

M

A

V

E

K

K

A

L

L

G

G

D

E

-

H

F

L

I

F

P

E

C

H

Y

F

V

I

K

E

L

A

K

K

T

E

E

I

E

E

W

W

N

D

A

M

Y

F

A

R

R

T

H

Q

L

V

T

H

E

P

W

W

E

E

R

R

E

E

T

Q

T

Y

L

M

active site: D52 (≠ A49), E131 (= E123), E133 (= E125), E188 (= E180), E195 (= E187), H244 (= H236), R315 (= R313), E332 (= E326), R334 (= R328)
binding adenosine-5'-diphosphate: K43 (≠ G40), M50 (≠ G47), F198 (≠ W190), Y200 (≠ F192), N246 (≠ H238), S248 (= S240), S324 (vs. gap), S328 (≠ A322), R330 (= R324)
binding glutamic acid: E133 (= E125), E188 (= E180), V189 (≠ D181), N239 (≠ T231), G240 (= G232), G242 (= G234), E303 (≠ T295)
binding magnesium ion: E131 (= E123), E188 (= E180), E195 (= E187), H244 (= H236), E332 (= E326)

4lniA B. Subtilis glutamine synthetase structures reveal large active site conformational changes and basis for isoenzyme specific regulation: structure of the transition state complex (see paper)
31% identity, 99% coverage: 5:430/432 of query aligns to 8:440/443 of 4lniA

query
sites
4lniA

L

I

H

E

A

K

V

L

A

V

C

K

E

E

R

E

G

N

I

V

K

K

Y

Y

F

I

L

R

I

L

S

Q

Y

F

T

T

D

D

L

I

F

L

G

G

V

T

Q

I

R

K

A

N

K

V

L

E

V

I

P

P

A

V

A

S

A

Q

I

L

D

G

G

K

M

A

V

L

K

D

T

N

G

K

A

V

G

M

F

F

A
x
D

G

G

F

S

A

S

T

I

W

E

L

G

D

F

M

V

T

R

P

I

A

E

D

E

P

S

D

D

V

M

F

Y

A

L

M

Y

P

P

D

D

P

L

A

N

S

T

L

F

I

V

Q

I

L

F

P

P

W

W

K

T

P

A

E

E

V

K

G

G

W

K

L

V

A

A

-

R

-

F

-

I

A

C

D

D

P

I

W

Y

M

N

-

P

N

D

G

G

K

T

P

P

V

F

A

E

H

G

A

D

P

P

R

R

N

N

L

N

L

L

K

K

R

R

M

I

V

L

A

K

D

E

A

M

A

E

A

D

Q

L

G

G

Y

F

-

S

E

D

L

F

K

N

S

L

G

G

V

P

E
|
E

C

P

E
|
E

F

F

L

L

I

F

T

K

A

L

D

D

G

E

S

K

A

G

P

E

S

P

D

T

V

L

A

E

D

L

K

N

Q

D

S

K

K

G

P

G

C

Y

Y

F

D

D

Q

L

Q

A

A

P

L

T

M

D

R

L

R

G

Y

E

E

N

V

C

I

R

A

R

E

D

I

I

C

V

D

L

A

E

M

L

L

E

A

E

L

M

G

G

W

F

K

E

P

I

Y
x
E

Q

A

N

S

D

H

H

H

E
|
E

D

V

A

A

N

P

G

G

Q

Q

F

H

E
|
E

M

I

N
x
D

W

F

E

K

F
x
Y

D

A

T

G

A

A

L

V

T

R

T

S

A

C

D

D

R

D

H

I

A

Q

F

T

F

F

K

K

Y

L

M

V

V

V

R

K

S

T

I

I

A

A

E

R

K

K

H

H

G

G

L

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

V

F

D

G

L

V

T

N

G

G

N

S

G

G

C

M

H
|
H

A

C

H
x
N

V

L

S
|
S

V

L

W

F

K

K

D

N

G

G

R

V

N

N

L

A

F

F

E

F

D

D

E

E

A

N

G

A

E

D

L

L

G

Q

V

L

S

S

E

E

L

T

G

A

Y

K

Q

H

F

F

L

I

G

A

G

G

I

I

M

V

H

K

S

H

A

A

D

T

A

S

L

F

A

T

A

A

L

V

F

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R
|
R

I

L

N

V

A

P

A

G

R

Y

T
x
E

T

-

S

-

G

-

A

-

T

-

W

-

A

A

P

P

N

C

S

Y

V

V

T

A

Y

W

S

S

G

A

N

Q

N

N

R
|
R

T

S

H

P

L

L

I

I

R
|
R

I

I

P

P

E

A

-

S

-

R

-

G

-

I

A

S

G

T

R
|
R

F

V

E
|
E

V

V

R
|
R

L

S

A

V

D

D

G

P

A

A

N

N

P

P

Y

Y

L

L

L

A

Q

L

A

S

G

V

L

L

A

A

G

G

L

L

D

D

G

G

I

I

A

K

N

N

D

K

R

L

D

E

P

A

G

P

P

A

R

P

L

I

D

D

I

R

N

N

M

I

Y

Y

T

V

D

M

G

S

H

K

A

E

V

E

K

R

T

M

G

E

K

N

-

G

-

I

-

V

K

D

L

L

P

P

L

A

N

T

L

L

L

A

D

E

A

A

I

L

R

E

A

E

L

F

Q

K

A

S

S

N

P

E

V

V

M

M

A

V

E

K

K

A

L

L

G

G

D

E

-

H

F

L

I

F

P

E

C

H

Y

F

V

I

K

E

L

A

K

K

T

E

E

I

E

E

W

W

N

D

A

M

Y

F

A

R

R

T

H

Q

L

V

T

H

E

P

W

W

E

E

R

R

E

E

T

Q

T

Y

L

M

active site: D52 (≠ A49), E131 (= E123), E133 (= E125), E188 (= E180), E195 (= E187), H244 (= H236), R315 (= R313), E332 (= E326), R334 (= R328)
binding adenosine-5'-diphosphate: E131 (= E123), E183 (≠ Y175), D197 (≠ N189), Y200 (≠ F192), N246 (≠ H238), S248 (= S240), R320 (= R318), R330 (= R324)
binding magnesium ion: E131 (= E123), E131 (= E123), E133 (= E125), E188 (= E180), E195 (= E187), E195 (= E187), H244 (= H236), E332 (= E326)
binding l-methionine-s-sulfoximine phosphate: E133 (= E125), E188 (= E180), H244 (= H236), R297 (= R289), E303 (≠ T295), R315 (= R313), R334 (= R328)

P12425 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Bacillus subtilis (strain 168) (see 5 papers)
31% identity, 99% coverage: 5:430/432 of query aligns to 9:441/444 of P12425

query
sites
P12425

L

I

H

E

A

K

V

L

A

V

C

K

E

E

R

E

G

N

I

V

K

K

Y

Y

F

I

L

R

I

L

S

Q

Y

F

T

T

D

D

L

I

F

L

G

G

V

T

Q

I

R

K

A

N

K

V

L

E

V

I

P

P

A

V

A

S

A

Q

I

L

D

G

G

K

M

A

V

L

K

D

T

N

G

K

A

V

G

M

F

F

A

D

G

G

F

S

A

S

T

I

W

E

L
x
G

D

F

M

V

T
x
R

P

I

A

E

D

E

P

S

D

D

V

M

F

Y

A

L

M

Y

P

P

D

D

P

L

A

N

S

T

L

F

I

V

Q

I

L

F

P

P

W

W

K

T

P

A

E

E

V

K

G

G

W

K

L

V

A

A

-

R

-

F

-

I

A

C

D

D

P

I

W

Y

M

N

-

P

N

D

G

G

K

T

P

P

V

F

A

E

H

G

A

D

P

P

R

R

N

N

L

N

L

L

K

K

R

R

M

I

V

L

A

K

D

E

A

M

A

E

A

D

Q

L

G

G

Y

F

-

S

E

D

L

F

K

N

S

L

G

G

V

P

E
|
E

C

P

E
|
E

F

F

L

L

I

F

T

K

A

L

D

D

G

E

S

K

A

G

P

E

S

P

D

T

V

L

A

E

D

L

K

N

Q

D

S

K

K

G

P

G

C

Y

Y

F

D

D

Q

L

Q

A

A

P

L

T

M

D

R

L

R

G

Y

E

E

N

V

C

I

R

A

R

E

D

I

I

C

V

D

L

A

E

M

L

L

E

A

E

L

M

G

G

W

F

K

E

P

I

Y

E

Q

A

N

S

D

H

H

H

E
|
E

D
x
V

A

A

N

P

G

G

Q

Q

F

H

E
|
E

M

I

N

D

W

F

E

K

F

Y

D

A

T

G

A

A

L

V

T

R

T

S

A

C

D

D

R

D

H

I

A

Q

F

T

F

F

K

K

Y

L

M

V

V

V

R

K

S

T

I

I

A

A

E

R

K

K

H

H

G

G

L

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

V

F

D

G

L

V

T

N

G
|
G

N

S

G

G

C

M

H
|
H

A

C

H

N

V

L

S

S

V

L

W

F

K

K

D

N

G

G

R

V

N

N

L

A

F

F

E

F

D

D

E

E

A

N

G

A

E

D

L

L

G

Q

V

L

S

S

E

E

L

T

G

A

Y

K

Q

H

F

F

L

I

G

A

G

G

I

I

M

V

H

K

S

H

A

A

D

T

A

S

L

F

A

T

A

A

L

V

F

T

N

N

P

P

T

T

V

V

N

N

S

S

Y

Y

K

K

R

R

I

L

N

V

A

P

A
x
G

R

Y

T
x
E

T

-

S

-

G

-

A

-

T

-

W

-

A

A

P
|
P

N

C

S

Y

V

V

T

A

Y

W

S

S

G

A

N

Q

N

N

R

R

T

S

H

P

L

L

I

I

R

R

I

I

P

P

E

A

-

S

-

R

-

G

-

I

A

S

G

T

R

R

F

V

E
|
E

V

V

R

R

L

S

A

V

D

D

G

P

A

A

N

N

P

P

Y

Y

L

L

L

A

Q

L

A

S

G

V

L

L

A

A

G

G

L

L

D

D

G

G

I

I

A

K

N

N

D

K

R

L

D

E

P

A

G

P

P

A

R

P

L

I

D

D

I

R

N

N

M

I

Y

Y

T

V

D

M

G

S

H

K

A

E

V

E

K

R

T

M

G

E

K

N

-

G

-

I

-

V

K

D

L

L

P

P

L

A

N

T

L

L

L

A

D

E

A

A

I

L

R

E

A

E

L

F

Q

K

A

S

S

N

P

E

V

V

M

M

A

V

E

K

K

A

L

L

G

G

D

E

-

H

F

L

I

F

P

E

C

H

Y

F

V

I

K

E

L

A

K

K

T

E

E

I

E
|
E

W

W

N

D

A

M

Y

F

A

R

R

T

H

Q

L

V

T

H

E

P

W

W

E

E

R

R

E

E

T

Q

T

Y

L

M

G59 (≠ L55) mutation to R: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion.
R62 (≠ T58) Important for inhibition by glutamine; mutation to A: Highly resistant to inhibition by glutamine and AMP. Regulation by TnrA and GlnR is abolished. Only small differences (less than 2-fold) in its steady-state kinetic constants compared with the wild-type. Similar sensitivity to Met-Sox that compared to the wild-ytpe.
E132 (= E123) binding Mg(2+)
E134 (= E125) binding Mg(2+)
E189 (= E180) binding Mg(2+)
V190 (≠ D181) mutation to A: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant partially relieves expression of the glnRA-lacZ fusion, but has no effect on the TnrA-dependent regulation of amtB-lacZ fusion. Resistant to inhibition by MetSox.
E196 (= E187) binding Mg(2+)
G241 (= G232) binding L-glutamate
H245 (= H236) binding Mg(2+)
G302 (≠ A293) mutation to E: Unable to form stable complex with TnrA. In the presence of glutamine, amtB-lacZ fusion is only 4-fold regulated by TnrA, whereas glnRA-lacZ fusion is derepressed. This mutant retains enzymatic specific activity with a 2-fold decrease of the affinity for glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.
E304 (≠ T295) mutation to A: Highly resistant to Met-Sox inhibition. 8- and 2-fold increase of the affinity for glutamate and ATP, respectively. Strong decrease of the affinity for ammonium.
P306 (= P303) mutation to H: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant completely derepresses glnRA-lacZ fusion, whereas amtB-lacZ fusion expression is only partially derepresses.
E333 (= E326) binding Mg(2+)
E424 (= E413) mutation to K: Unable to form stable complex with TnrA. In the presence of glutamine, this mutant derepresses amtB-lacZ fusion and glnRA-lacZ fusion. Although it is defective in regulation, this mutant retains enzymatic specific activity and similar affinity for ATP, glutamate and glutamine compared to the wild-type. Slightly less sensitive to inhibition by glutamine.

Sites not aligning to the query:

1 modified: Initiator methionine, Removed

7tf6A Glutamine synthetase (see paper)
31% identity, 99% coverage: 5:430/432 of query aligns to 7:435/438 of 7tf6A

query
sites
7tf6A

L

I

H

R

A

K

V

F

A

A

C

E

E

E

R

E

G

N

I

V

K

R

Y

Y

F

L

L

R

I

L

S

Q

Y

F

T

T

D

D

L

I

F

L

G

G

V

T

Q

I

R

K

A

N

K

V

L

E

V

V

P

P

A

V

A

S

A

Q

I

L

D

E

G

K

M

V

V

L

K

D

T

N

G

E

A

M

G

M

F

F

A

D

G

G

F

S

A

S

T

I

W

E

L

G

D
x
F

M

V

T
x
R

P

I

A

E

D

E

P

S

D

D

V

M

F

Y

A

L

M

H

P

P

D

D

P

L

A

D

S

T

L

W

I

V

Q

I

L

F

P

P

W

W

K

G

P

-

E

K

V

V

G

A

W

R

L

L

A

I

A

C

D

D

P

V

W

Y

-

K

M

T

N

D

G

G

K

T

P

P

V

F

A

E

H

G

A

D

P

P

R

R

N

A

L

N

L

L

K

K

R

R

M

V

V

L

A

K

D

E

A

M

A

E

A

D

Q

L

G

G

Y

F

-

T

E

D

L

F

K

N

S

L

G

G

V

P

E
|
E

C

P

E
|
E

F

F

L

L

I

F

T

K

A

L

D

D

G

E

S

K

-

G

-

E

-

P

-

T

-

L

-

E

-

L

-

N

-

D

-

G

-

Y

-

F

-

D

-

L

A

A

P

P

S

T

D

D

V

L

A

G

D

E

K

N

Q

-

S

-

K

-

P

-

C

C

Y

-

D

-

Q

-

A

-

L

-

M

-

R

-

R

R

Y

R

E

D

V

I

I

V

A

L

E

E

I

L

C

E

D

D

A

-

M

-

L

-

A

-

L

M

G

G

W

F

K

D

P

I

Y

E

Q

A

N

S

D

H

H

H

E
|
E

D

V

A

A

N

P

G

G

Q

Q

F

H

E
|
E

M

I

N

D

W

F

E

K

F

Y

D

A

T

D

A

A

L

V

T

T

T

A

A

C

D

D

R

N

H

I

A

Q

F

T

F

F

K

K

Y

L

M

V

V

V

R

K

S

T

I

I

A

A

E

R

K

K

H

H

G

N

L

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

V

F

D
x
G

L

V

T
x
N

G
|
G

N

S

G

G

C

M

H
|
H

A

F

H

N

V

V

S

S

V

L

W

F

K

K

D

G

G

K

R

E

N

N

L

A

F

F

E

F

D

D

E

P

A

N

G

T

E

E

L

M

G

G

V

L

S

T

E

E

L

T

G

A

Y

Y

Q

Q

F

F

L

T

G

A

G

G

I

V

M

L

H

K

S

N

A

A

D

R

A

G

L

F

A

T

A

A

L

V

F

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

I

L

N

V

A

P

A
x
G

R
x
Y

T
x
E

T

-

S

-

G

-

A

-

T

-

W

-

A

A

P

P

N

C

S

Y

V

I

T

A

Y

W

S

S

G

G

N

K

N

N

R
|
R

T

S

H

P

L

L

I

I

R

R

I

V

P

P

E

S

-

S

-

R

-

G

-

L

A

S

G

T

R

R

F

I

E
|
E

V

V

R

R

L

S

A

V

D

D

G

P

A

A

N

N

P

P

Y

Y

L

M

L

A

Q

L

A

A

G

A

L

I

L

L

A

E

A

A

G

G

L

L

D

D

G

G

I

I

A

K

N

N

D

K

R

L

D

K

P

V

G

P

P

E

R

P

L

V

D

N

I

Q

N

N

M

I

Y
|
Y

T

E

D

M

G

N

H

R

A

E

V

E

K

R

T

E

G

A

-

V

-

G

-

I

K

Q

K

D

L

L

P

P

L

S

N

T

L

L

L

Y

D

T

A

A

I

L

R

K

A

A

L

M

Q

R

A

E

S

N

P

E

V

V

M

I

A

K

E

K

K

A

L

L

G

G

D

N

F

H

I

I

-

Y

P

N

C

Q

Y

F

V

I

K

N

L

S

K

K

T

S

E

I

E

E

W

W

N

D

A
x
Y

Y

Y

A

R

R

T

H

Q

L

V

T

S

E

E

W

W

E

E

R

R

E

D

T
x
Q

T

Y

L

M

binding glutamine: E128 (= E125), E183 (= E180), G235 (= G232), H239 (= H236), R292 (= R289), E298 (≠ T295)
binding magnesium ion: E126 (= E123), E128 (= E125), E183 (= E180), E190 (= E187), H239 (= H236), E327 (= E326)
binding : F58 (≠ D56), R60 (≠ T58), G232 (≠ D229), N234 (≠ T231), G296 (≠ A293), Y297 (≠ R294), R310 (= R313), Y367 (= Y366), Y421 (≠ A416), Q433 (≠ T428)

Sites not aligning to the query:

binding : 437

7tdvC Glutamine synthetase (see paper)
31% identity, 97% coverage: 11:430/432 of query aligns to 14:440/443 of 7tdvC

query
sites
7tdvC

E

E

R

E

G

N

I

V

K

R

Y

Y

F

L

L

R

I

L

S

Q

Y

F

T

T

D

D

L

I

F

L

G

G

V

T

Q

I

R

K

A

N

K

V

L

E

V

V

P

P

A

V

A

S

A

Q

I

L

D

E

G

K

M

V

V

L

K

D

T

N

G

E

A

M

G

M

F

F

A

D

G

G

F

S

A

S

T

I

W

E

L

G

D

F

M

V

T

R

P

I

A

E

D

E

P

S

D

D

V

M

F

Y

A

L

M

H

P

P

D

D

P

L

A

D

S

T

L

W

I

V

Q

I

L

F

P

P

W

W

-

T

-

A

-

G

K

Q

P

G

E

K

V

V

G

A

W

R

L

L

A

I

A

C

D

D

P

V

W

Y

-

K

M

T

N

D

G

G

K

T

P

P

V

F

A

E

H

G

A

D

P

P

R

R

N

A

L

N

L

L

K

K

R

R

M

V

V

L

A

K

D

E

A

M

A

E

A

D

Q

L

G

G

Y

F

-

T

E

D

L

F

K

N

S

L

G
|
G

V

P

E
|
E

C

P

E
|
E

F

F

L

L

I

F

T

K

A

L

D

D

G

E

S

K

-

G

-

E

-

P

-

T

-

L

-

E

-

L

-

N

-

D

-

G

-

Y

-

F

-

D

-

L

A

A

P

P

S

T

D

D

V

L

A

G

D

E

K

N

Q

-

S

-

K

-

P

-

C

C

Y

-

D

-

Q

-

A

-

L

-

M

-

R

-

R

R

Y

R

E

D

V

I

I

V

A

L

E

E

I

L

C

E

D

D

A

-

M

-

L

-

A

-

L

M

G

G

W

F

K

D

P

I

Y
x
E

Q

A

N

S

D

H

H

H

E
|
E

D

V

A

A

N

P

G

G

Q

Q

F

H

E
|
E

M

I

N
x
D

W
x
F

E
x
K

F
x
Y

D

A

T

D

A

A

L

V

T

T

T

A

A

C

D

D

R

N

H

I

A

Q

F

T

F

F

K

K

Y

L

M

V

V

V

R

K

S

T

I

I

A

A

E

R

K

K

H

H

G

N

L

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

V

F

D

G

L

V

T

N

G
|
G

N

S

G

G

C

M

H
|
H

A

F

H
x
N

V
|
V

S
|
S

V

L

W

F

K

K

D

G

G

K

R

E

N

N

L

A

F

F

E

F

D

D

E

P

A

N

G

T

E

E

L

M

G

G

V

L

S

T

E

E

L

T

G

A

Y

Y

Q

Q

F

F

L

T

G

A

G

G

I

V

M

L

H

K

S

N

A

A

D

R

A

G

L

F

A

T

A

A

L

V

F

C

N

N

P

P

T

L

V

V

N

N

S

S

Y

Y

K

K

R
|
R

I

L

N

V

A

P

A

G

R

Y

T
x
E

T

-

S

-

G

-

A

-

T

-

W

-

A

A

P

P

N

C

S

Y

V

I

T

A

Y

W

S

S

G

G

N

K

N

N

R
|
R

T

S

H

P

L

L

I

I

R
|
R

I

V

P

P

E

S

-

S

-

R

-

G

-

L

A
x
S

G

T

R
|
R

F

I

E
|
E

V

V

R

R

L

S

A

V

D

D

G

P

A

A

N

N

P

P

Y

Y

L

M

L

A

Q

L

A

A

G

A

L

I

L

L

A

E

A

A

G

G

L

L

D

D

G

G

I

I

A

K

N

N

D

K

R

L

D

K

P

V

G

P

P

E

R

P

L

V

D

N

I

Q

N

N

M

I

Y

Y

T

E

D

M

G

N

H

R

A

E

V

E

K

R

T

E

G

A

-

V

-

G

-

I

K

Q

K

D

L

L

P

P

L

S

N

T

L

L

L

Y

D

T

A

A

I

L

R

K

A

A

L

M

Q

R

A

E

S

N

P

E

V

V

M

I

A

K

E

K

K

A

L

L

G

G

D

N

F

H

I

I

-

Y

P

N

C

Q

Y

F

V

I

K

N

L

S

K

K

T

S

E

I

E

E

W

W

N

D

A

Y

Y

Y

A

R

R

T

H

Q

L

V

T

S

E

E

W

W

E

E

R

R

E

D

T

Q

T

Y

L

M

binding adenosine-5'-diphosphate: G129 (= G121), E131 (= E123), E183 (≠ Y175), D197 (≠ N189), F198 (≠ W190), K199 (≠ E191), Y200 (≠ F192), N246 (≠ H238), V247 (= V239), S248 (= S240), R320 (= R318), S328 (≠ A322), R330 (= R324)
binding magnesium ion: E131 (= E123), E131 (= E123), E133 (= E125), E188 (= E180), E195 (= E187), E195 (= E187), H244 (= H236), E332 (= E326)
binding l-methionine-s-sulfoximine phosphate: E131 (= E123), E133 (= E125), E188 (= E180), E195 (= E187), G240 (= G232), H244 (= H236), R297 (= R289), E303 (≠ T295), R315 (= R313)

7tenA Glutamine synthetase (see paper)
31% identity, 98% coverage: 9:432/432 of query aligns to 11:441/442 of 7tenA

query
sites
7tenA

A

A

C

D

E

E

R

Q

G

N

I

V

K

K

Y

F

F

I

L

R

I

L

S

Q

Y

F

T

T

D

D

L

I

F

L

G

G

V

I

Q

I

R

K

A

N

K

V

L

E

V

I

P

P

A

V

A

S

A

Q

I

L

D

K

G

K

M

A

V

L

K

D

T

N

G

K

A

I

G

M

F

F

A

D

G

G

F

S

A

S

T

I

W

E

L

G

D

F

M

V

T

R

P

I

A

E

D

E

P

S

D

D

V

M

F

Y

A

L

M

F

P

P

D

D

P

L

A

D

S

T

L

W

I

V

Q

V

L

F

P

P

W

W

K

T

P

A

E

E

V

K

G

G

W

K

L

V

A

A

A

R

-

M

-

I

-

C

-

D

-

I

-

Y

D

N

P

P

W

D

M

M

N

T

G

-

K

-

P

P

V

F

A

A

H

G

A

D

P

P

R

R

N

A

L

N

L

L

K

K

R

R

M

V

V

L

A

K

D

E

A

M

A

E

Q

L

G

G

Y

F

-

T

E

E

L

F

K

N

S

L

G
|
G

V

P

E
|
E

C

P

E
|
E

F

F

L

L

I

F

T

K

A

L

D

D

G

E

S

N

A

R

P

R

S

P

D

T

V

L

A

E

D

L

K

N

Q

D

S

S

K

G

P

G

C

Y

Y

F

D

D

Q

L

Q

A

A

P

L

T

M

D

R

L

R

G

Y

E

E

N

V

C

I

R

A

R

E

D

I

I

C

V

D

L

A

E

M

L

L

E

A

E

L

M

G

G

W

F

K

E

P

I

Y
x
E

Q

A

N

S

D

H

H

H

E
|
E

D

V

A

A

N

P

G

G

Q

Q

F

H

E
|
E

M

I

N
x
D

W
x
F

E
x
K

F
x
Y

D

E

T

D

A

A

L

I

T

T

T

A

A

C

D

D

R

S

H

I

A

Q

F

T

F

F

K

K

Y

L

M

V

V

V

R

K

S

T

I

I

A

A

E

R

K

K

H

H

G

G

L

L

R

H

A

A

T

T

F

F

M

M

P

P

K

K

P

P

F

L

V

F

D

G

L

V

T
x
N

G
|
G

N

S

G

G

C

M

H
|
H

A

F

H
x
N

V

M

S
|
S

V

L

W

F

K

N

D

E

G

K

R

G

N

N

L

A

F

F

E

F

D

D

E

E

A

S

G

G

E

E

L

L

G

E

V

L

S

S

E

Q

L

T

G

A

Y

Y

Q

H

F

F

L

L

G

A

G

G

I

M

M

L

H

K

S

H

A

A

D

R

A

G

L

Y

A

T

A

A

L

V

F

T

N

N

P

P

T

T

V

I

N

N

S

S

Y

F

K

K

R
|
R

I

L

N

V

A

P

A

G

R

Y

T
x
E

T

-

S

-

G

-

A

-

T

-

W

-

A

A

P

P

N

C

S

Y

V

I

T

A

Y

W

S

S

G

G

N

K

N

N

R
|
R

T

S

H

P

L

L

I

V

R
|
R

I

V

P

P

E

S

-

S

-

R

-

G

-

L

A
x
S

G

T

R
|
R

F

L

E

E

V

L

R
|
R

L

S

A

V

D

D

G

P

A

S

A

A

N

N

P

P

Y

Y

L

L

L

A

Q

M

A

A

G

V

L

L

A

K

A

A

G

G

L

L

D

S

G

G

I

I

A

K

N

D

D

E

R

L

D

T

P

P

G

P

P

A

R

P

L

V

D

D

I

R

N

N

M

I

Y

Y

-

G

-

M

T

N

D

E

G

E

H

E

A

R

V

E

K

A

T

T

G

G

-

I

K

Y

K

D

L

L

P

P

L

E

N

S

L

L

L

G

D

H

A

A

I

L

R

I

A

E

L

L

Q

E

A

K

S

N

P

E

V

I

M

I

A

K

E

D

K

G

L

L

G

G

D

E

F

H

I

I

-

F

P

E

C

H

Y

F

V

I

K

E

L

A

K

K

T

T

E

I

E

E

W

C

N

D

A

M

Y

F

A

R

R

T

H

A

L

V

T

H

E

P

W

W

E

E

R

R

E

E

T

Q

T

Y

L

L

D

E

C

I

binding adenosine-5'-diphosphate: G128 (= G121), E130 (= E123), E182 (≠ Y175), D196 (≠ N189), F197 (≠ W190), K198 (≠ E191), Y199 (≠ F192), N245 (≠ H238), S247 (= S240), R319 (= R318), S327 (≠ A322), R329 (= R324)
binding l-methionine-s-sulfoximine phosphate: E130 (= E123), E132 (= E125), E187 (= E180), E194 (= E187), N238 (≠ T231), G239 (= G232), H243 (= H236), R296 (= R289), E302 (≠ T295), R314 (= R313), R333 (= R328)

P9WN37 Glutamine synthetase; GS; Glutamate--ammonia ligase; Glutamine synthetase I alpha; GSI alpha; EC 6.3.1.2 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
32% identity, 97% coverage: 11:430/432 of query aligns to 14:444/446 of P9WN37

query
sites
P9WN37

E

E

R

R

G

D

I

I

K

R

Y

F

F

V

L

R

I

L

S

W

Y

F

T

T

D

D

L

V

F

L

G

G

V

F

Q

L

R

K

A

S

K

V

L

A

V

I

P

A

A

P

A

A

A

E

I

L

D

E

G

G

M

A

V

F

K

E

T

E

G

G

A

I

G

G

F

F

A

D

G

G

F

S

A

S

T

I

W

E

L

G

D

F

M

A

T

R

P

V

A

S

D

E

P

S

D

D

V

T

F

V

A

A

M

H

P

P

D

D

P

P

A

S

S

T

L

F

I

Q

Q

V

L

L

P

P

W

W

K

A

P

T

E

S

V

S

G

G

W

H

-

H

-

S

-

A

-

R

L

M

A

F

A

C

D

D

P

I

W

T

M

M

-

P

N

D

G

G

K

S

P

P

V

S

A

W

H

A

A

D

P

P

R

R

N

H

L

V

L

L

K

R

R

R

M

Q

V

L

A

T

D

K

A

A

A

G

A

E

Q

L

G

G

Y

F

E

S

L

C

K

Y

S

V

G

H

V

P

E

E

C

I

E

E

F

F

L

L

I

L

T

K

-

P

-

G

-

P

A

E

D

D

G

G

S

S

A

V

P

P

S

V

-

P

-

V

D

D

V

N

A

A

D

G

K

Y

Q

F

S

D

K

Q

P

A

C

V

Y

H

D

D

Q

S

Q

A

A

L

L

N

M

F

R

R

Y

H

E

A

V

I

I

-

A

-

E

-

I

-

C

-

D

D

A

A

M

L

L

E

A

F

L

M

G

G

W

I

K

S

P

V

Y

E

Q

F

N

S

D

H

H

H

E

E

D

G

A

A

N

P

G

G

Q

Q

F

Q

E

E

M

I

N

D

W

L

E

R

F

F

D

A

T

D

A

A

L

L

T

S

T

M

A

A

D

D

R

N

H

V

A

M

F

T

F

F

K

R

Y

Y

M

V

V

I

R

K

S

E

I

V

A

A

E

L

K

E

H

E

G

G

L

A

R

R

A

A

T

S

F

F

M

M

P

P

K

K

P

P

F

F

V

G

D

Q

L

H

T

P

G

G

N

S

G

A

C

M

H

H

A

T

H

H

V

M

S

S

V

L

W

F

K

E

D

G

G

D

R

V

N

N

L

A

F

F

E

H

D

S

E

A

A

D

G

D

E

P

L

L

G

Q

V

L

S

S

E

E

L

V

G

G

Y

K

Q

S

F

F

L

I

G

A

G

G

I

I

M

L

H

E

S

H

A

A

D

C

A

E

L

I

A

S

A

A

L

V

F

T

N

N

P

Q

T

W

V

V

N

N

S

S

Y

Y

K

K

R

R

I

L

N

-

A

-

R

-

T

-

T

V

S

Q

G

G

A

G

T

E

W

-

A

A

P

P

N

T

S

A

V

A

T

S

Y

W

S

G

G

A

N

A

N

N

R

R

T

S

H

A

L

L

I

V

R

R

I

V

P

P

-

M

-

Y

-

T

-

P

-

H

-

K

-

T

E

S

A

S

G

R

R

R

F

V

E

E

V

V

R

R

L

S

A

P

D

D

G

S

A

A

A

C

N

N

P

P

Y

Y

L

L

L

T

Q

F

A

A

G

V

L

L

A

A

G

G

L

L

D

R

G

G

I

V

A

E

N
x
K

D

G

R

Y

D

V

P

L

G

G

P

P

R

Q

L

A

D

E

I

D

N

N

M

V

Y

W

T

D

-

L

-

T

-

P

D

E

G

E

H

R

A

R

V

A

K

M

T

G

G

Y

K

R

K

E

L

L

P

P

L

S

N

S

L

L

L

D

D

S

A

A

I

L

R

R

A

A

L

M

Q

E

A

A

S

S

P

E

V

L

M

V

A

A

E

E

K

A

L

L

G

G

D

E

F

H

I

V

-

F

P

D

C

F

Y

F

V

L

K

R

L

N

K

K

T

R

E

T

E

E

W

W

N

A

A

N

Y

Y

A

R

R

S

H

H

L

V

T

T

E

P

W

Y

E

E

R

L

E

R

T

T

T

Y

L

L

K363 (≠ N353) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Query Sequence

>WP_012042168.1 NCBI__GCF_000015165.1:WP_012042168.1
MSSSLHAVACERGIKYFLISYTDLFGVQRAKLVPAAAIDGMVKTGAGFAGFATWLDMTPA
DPDVFAMPDPASLIQLPWKPEVGWLAADPWMNGKPVAHAPRNLLKRMVADAAAQGYELKS
GVECEFFLITADGSAPSDVADKQSKPCYDQQALMRRYEVIAEICDAMLALGWKPYQNDHE
DANGQFEMNWEFDTALTTADRHAFFKYMVRSIAEKHGLRATFMPKPFVDLTGNGCHAHVS
VWKDGRNLFEDEAGELGVSELGYQFLGGIMHSADALAALFNPTVNSYKRINAARTTSGAT
WAPNSVTYSGNNRTHLIRIPEAGRFEVRLADGAANPYLLQAGLLAAGLDGIANDRDPGPR
LDINMYTDGHAVKTGKKLPLNLLDAIRALQASPVMAEKLGDFIPCYVKLKTEEWNAYARH
LTEWERETTLDC

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory