SitesBLAST

M1 (= M1) modified: Initiator methionine, Removed
C2 (= C2) mutation C->A,S: Loss of glutamine-dependent activity but no effect on ammonia-dependent asparagine synthetase activity.
H30 (= H31) mutation to A: 4,5-fold decrease in glutamine affinity.
D34 (= D35) mutation D->N,E: Little effect on the kinetic properties.
H81 (= H85) mutation to A: 5-fold decrease in glutamine affinity.
A105 (= A112) mutation to H: Little effect on the kinetic properties.
E349 (≠ D380) mutation E->A,Q: Loss of glutamine- and ammonia-dependent synthetase activity, but still exhibits glutaminase activity.; mutation to D: 5-fold increase in affinity for aspartate when assaying both the glutamine- and ammonia-dependent synthetase reactions, and 2-fold decrease in kcat for these reactions. Modifies the product glutamate/asparagine stoichiometry.

1ct9A Crystal structure of asparagine synthetase b from escherichia coli (see paper)
31% identity, 56% coverage: 24:389/656 of query aligns to 22:341/497 of 1ct9A

query
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1ct9A

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active site: L50 (= L54), N74 (= N79), G75 (= G80), T305 (≠ S358), R308 (vs. gap), E332 (≠ D380)
binding adenosine monophosphate: L232 (≠ F278), L233 (= L279), S234 (= S280), S239 (= S285), A255 (≠ S304), V256 (≠ I305), D263 (≠ E314), M316 (≠ V365), S330 (= S378), G331 (= G379), E332 (≠ D380)
binding glutamine: R49 (= R53), L50 (= L54), I52 (= I56), V53 (≠ L57), N74 (= N79), G75 (= G80), E76 (= E81), D98 (= D106)

Sites not aligning to the query:

active site: 1, 366
binding glutamine: 1

P08243 Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Homo sapiens (Human) (see 7 papers)
30% identity, 59% coverage: 1:389/656 of query aligns to 1:374/561 of P08243

query
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P08243

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C2 (= C2) active site, For GATase activity; mutation to A: Loss of the glutamine-dependent asparagine synthetase activity, while the ammonia-dependent activity remained unaffected.
A6 (≠ G6) to E: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122975
V210 (≠ T238) to E: in dbSNP:rs1049674
F362 (≠ L377) to V: in ASNSD; dramatic reduction in protein abundance; dbSNP:rs398122973

Sites not aligning to the query:

550 R → C: in ASNSD; increases level of protein abundance; dbSNP:rs398122974

6gq3A Human asparagine synthetase (asns) in complex with 6-diazo-5-oxo-l- norleucine (don) at 1.85 a resolution (see paper)
30% identity, 59% coverage: 2:389/656 of query aligns to 1:361/509 of 6gq3A

query
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6gq3A

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active site: W4 (≠ A5), L49 (= L54), N74 (= N79), G75 (= G80), T324 (≠ P353), R327 (≠ D356)
binding 5-oxo-l-norleucine: C1 (= C2), R48 (= R53), V51 (≠ I56), V52 (≠ L57), Y73 (≠ F78), N74 (= N79), G75 (= G80), E76 (= E81), V95 (≠ S105), D96 (= D106)

P78753 Probable asparagine synthetase [glutamine-hydrolyzing]; Glutamine-dependent asparagine synthetase; EC 6.3.5.4 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
29% identity, 59% coverage: 1:389/656 of query aligns to 1:365/557 of P78753

query
sites
P78753

M

M

C

C

G

G

I

I

A

L

G

A

L

V

F

H

R

H

P

V

G

A

G

-

A

E

D

D

D

I

S

E

A

A

L

F

A

K

G

P

I

K

V

A

G

L

R

H

M

L

T

S

A

K

A

Q

L

L

A

R

H

H

R

R

G

G

P

P

D

D

A

W

S

S

G

G

S

K

W

A

V

I

S

-

G

-

R

R

Q

N

G

Q

I

T

A

I

L

L

G

C

Q

H

R

E

R

R

L

L

A

A

I

I

L

V

D

G

L

V

S

-

E

E

A

S

G

G

A

A

Q

Q

P

P

M

L

H

V

S

S

A

D

C

D

G

G

R

K

Y

L

T

V

I

L

T

T

F

V

N

N

G

G

E

E

I

I

Y

Y

N

N

H

H

L

L

D

K

L

L

R

R

A

-

E

-

L

-

E

E

D

N

A

L

A

K

A

G

A

N

P

Y

N

K

W

F

R

K

G

T

H

Y

S

S

D

D

T

C

E

E

T

V

L

I

L

L

A

Y

A

L

I

Y

R

R

Q

E

W

H

G

G

L

-

V

-

P

P

A

A

L

C

Q

A

R

N

L

M

I

L

-

D

G

G

M

M

F

F

A

S

F

W

A

V

L

L

W

Y

D

D

A

Q

E

D

T

K

R

D

Q

K

L

V

I

V

L

A

A

A

R

R

D

D

R

P

F

I

G

G

E

I

K

T

P

T

L

L

F

Y

Y

Q

G

G

W

F

S

S

G

S

A

D

D

S

-

P

-

D

-

T

L

A

V

Y

F

F

G

A

S

S

E

E

L

L

K

K

A

A

L

L

A

-

H

H

P

P

G

-

W

-

A

-

P

-

S

-

L

-

D

-

R

-

A

-

L

-

T

-

D

-

F

-

M

-

R

-

Y

-

S

-

Y

-

V

-

P

-

A

-

P

-

A

-

T

-

I

V

W

C

R

D

D

K

V

I

R

I

K

A

L

F

P

P

A

G

S

H

F

Y

V

-

A

-

F

Y

V

D

A

S

D

E

A

T

G

K

P

Q

G

T

T

V

S

R

P

Y

T

F

A

K

Q

P

S

S

Y

W

W

W

S

-

L

-

R

-

D

-

T

-

V

-

A

-

Q

D

G

E

D

N

R

K

I

I

S

P

G

S

E

N

S

P

E

V

A

D

I

Y

A

K

R

L

L

L

E

R

Q

E

L

T

L

L

S

E

T

A

A

S

V

V

K

R

R

K

Q

R

C

L

L

M

S

A

D

E

V

V

P

P

L

Y

G

G

A

V

F

L

L

L

S

S

G

G

V

L

D

D

S

S

T

L

I

I

V

A

A

S

L

I

M

A

Q

A

A

R

Q

E

A

T

-

E

-

K

-

L

-

A

-

N

-

S

-

T

-

S

-

Q

S

S

Q

E

P

E

V

A

R

R

T

T

-

I

-

T

-

A

-

W

-

P

-

K

-

L

-

H

-

S

F

F

S

A

I

I

G

G

F

L

A

P

E

-

G

-

G

G

Y

S

N

P

E

D

A

L

E

L

D

A

A

A

R

R

K

K

V

V

A

A

Q

D

H

F

L

L

G

H

T

T

D

F

H

H

T

H

E

E

L

H

Y

T

V

F

D

T

A

I

R

D

T

E

A

G

M

L

E

D

V

A

V

L

P

R

K

D

L

V

P

-

R

-

I

I

Y

Y

D

H

E

L

P

E

F

T

A

Y

D

D

S

V

S

T

Q

T

I

I

-

R

-

A

-

S

-

T

P

P

T

M

H

Y

L

L

V

L

A

S

-

R

Q

K

L

I

A

K

R

A

R

Q

H

G

V

V

T

K

V

M

A

V

L

L

S

S

G

G

D

E

A

G

G

S

D

D

E

E

L

I

F

F

G

G

Y

Y

Sites not aligning to the query:

391 modified: Phosphoserine
489 modified: Phosphoserine

1jgtB Crystal structure of beta-lactam synthetase (see paper)
30% identity, 42% coverage: 75:347/656 of query aligns to 69:307/500 of 1jgtB

query
sites
1jgtB

T

T

I

A

T

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

D

E

L

L

R

L

A

S

E

V

L

L

E

P

D

-

A

A

A

G

A

P

A

A

P

P

N

E

W

-

R

-

G

-

H

-

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

A

L

I

L

R

E

Q

R

W

Y

G

D

L

L

V

-

P

H

A

A

L

F

Q

R

R

L

L

V

I

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

A

G

E

D

T

-

R

-

Q

R

L

V

I

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

E

S

K

V

P

P

L

L

F

Y

Y

T

G

C

W

V

S

A

G

P

A

G

D

E

L

V

V

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

A

A

H

H

-

R

-

D

P

P

G

K

W

G

A

F

P

P

S

L

L

A

D

D

R

A

A

R

L

V

T

A

D

G

F

L

M

T

R

G

Y

V

S

Y

Y

Q

V

V

P

P

A

A

P

G

A

A

T

V

I

M

W

-

R

-

D

D

V

I

R

-

K

-

L

-

P

-

A

-

S

-

F

-

V

-

A

-

F

-

V

-

A

-

D

D

A

L

G

G

P

S

G

G

T

T

S

A

P

V

T

T

A

H

Q

R

S

T

Y

W

W

-

S

-

L

-

R

-

D

-

T

-

V

-

V

T

A

P

A

G

Q

L

G

S

D

R

R

R

I

I

S

L

G

P

E

E

S

G

E

E

A

A

I

V

A

A

R

A

L

V

E

R

Q

A

L

A

L

L

S

E

T

K

A

A

V

V

-

A

K

Q

R

R

Q

V

C

T

L

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V
x
I

D
|
D

S
|
S

S

S

T

G

I

V

V

A

A

A

L

C

M

A

Q

H

A

R

Q

A

A

A

S

G

Q

E

P

-

V

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

A

D

E

T

G

S

G

-

Y

-

N

N

E

E

A

F

E

R

D

E

A

A

R

R

K

A

V

V

A

V

Q

D

H

H

L

L

G

R

T

T

D

R

H

H

T

R

E

E

L

I

Y

T

V

I

D

-

A

-

R

-

T

P

A

T

M

T

E

E

V

L

P

A

K

Q

L

L

P

P

active site: A73 (≠ N79), G74 (= G80)
binding diphosphomethylphosphonic acid adenosyl ester: V244 (≠ F278), L245 (= L279), S246 (= S280), G248 (= G282), I249 (≠ V283), D250 (= D284), S251 (= S285), S269 (= S304), M270 (≠ I305)
binding magnesium ion: D250 (= D284)

Sites not aligning to the query:

active site: 319, 345, 379, 440
binding diphosphomethylphosphonic acid adenosyl ester: 327, 344, 345, 348, 420, 440
binding n2-(carboxyethyl)-l-arginine: 323, 345, 346, 348, 349, 354, 370, 379
binding magnesium ion: 348

1mbzA Beta-lactam synthetase with trapped intermediate (see paper)
30% identity, 42% coverage: 75:347/656 of query aligns to 65:299/496 of 1mbzA

query
sites
1mbzA

T

T

I

A

T

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

D

E

L

L

R

L

A

S

E

V

L

L

E

P

D

-

A

A

A

G

A

P

A

A

P

P

N

E

W

-

R

-

G

-

H

-

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

A

L

I

L

R

E

Q

R

W

Y

G

D

L

L

V

-

P

H

A

A

L

F

Q

R

R

L

L

V

I

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

A

G

E

D

T

-

R

-

Q

R

L

V

I

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

E

S

K

V

P

P

L

L

F

Y

Y

T

G

C

W

V

S

A

G

P

A

G

D

E

L

V

V

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

A

A

H

H

P

-

G

G

W

F

A

-

P

P

S

L

L

A

D

D

R

A

A

R

L

V

T

A

D

G

F

L

M

T

R

G

Y

V

S

Y

Y

Q

V

V

P

P

A

A

P

G

A

A

T

V

I

M

W

-

R

-

D

D

V

I

R

-

K

-

L

-

P

-

A

-

S

-

F

-

V

-

A

-

F

-

V

-

A

-

D

D

A

L

G

G

P

S

G

G

T

T

S

A

P

V

T

T

A

H

Q

R

S

T

Y

W

W

-

S

-

L

-

R

-

D

-

T

-

V

-

V

T

A

P

A

G

Q

L

G

S

D

R

R

R

I

I

S

L

G

P

E

E

S

G

E

E

A

A

I

V

A

A

R

A

L

V

E

R

Q

A

L

A

L

L

S

E

T

K

A

A

V

V

-

A

K

Q

R

R

Q

V

C

T

L

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

T

G

I

V

V

A

A

A

L

C

M

A

Q

H

A

R

Q

A

A

A

S

G

Q

E

P

-

V

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

A

D

E

T

G

S

G

-

Y

-

N

N

E

E

A

F

E

R

D

E

A

A

R

R

K

A

V

V

A

V

Q

D

H

H

L

L

G

R

T

T

D

R

H

H

T

R

E

E

L

I

Y

T

V

I

D

-

A

-

R

-

T

P

A

T

M

T

E

E

V

L

P

A

K

Q

L

L

P

P

active site: A69 (≠ N79), G70 (= G80)
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: V236 (≠ F278), L237 (= L279), S238 (= S280), S243 (= S285), S261 (= S304), M262 (≠ I305)
binding magnesium ion: D242 (= D284)
binding pyrophosphate 2-: S238 (= S280), G240 (= G282), D242 (= D284), S243 (= S285)

Sites not aligning to the query:

active site: 311, 337, 371, 432
binding arginine-n-methylcarbonyl phosphoric acid 5'-adenosine ester: 315, 319, 336, 337, 338, 340, 341, 362, 371, 432, 434, 435
binding magnesium ion: 340
binding pyrophosphate 2-: 340, 412, 432, 433

1mb9A Beta-lactam synthetase complexed with atp (see paper)
31% identity, 42% coverage: 75:347/656 of query aligns to 66:298/485 of 1mb9A

query
sites
1mb9A

T

T

I

A

T

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

D

E

L

L

R

L

A

S

E

V

L

L

E

P

D

-

A

A

A

G

A

P

A

A

P

P

N

E

W

-

R

-

G

-

H

-

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

A

L

I

L

R

E

Q

R

W

Y

G

D

L

L

V

-

P

H

A

A

L

F

Q

R

R

L

L

V

I

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

A

G

E

D

T

-

R

-

Q

R

L

V

I

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

E

S

K

V

P

P

L

L

F

Y

Y

T

G

C

W

V

S

A

G

P

A

G

D

E

L

V

V

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

A

A

H

F

P

P

G

-

W

L

A

A

P

D

S

A

L

R

D

R

R

V

A

A

A

G

L

L

T

T

D

G

F

V

M

Y

R

Q

Y

-

S

-

Y

-

V

V

P

P

A

A

P

G

A

A

T

V

I

M

W

-

R

-

D

D

V

I

R

-

K

-

L

-

P

-

A

-

S

-

F

-

V

-

A

-

F

-

V

-

A

-

D

D

A

L

G

G

P

S

G

G

T

T

S

A

P

V

T

T

A

H

Q

R

S

T

Y

-

W

W

S

T

L

-

R

-

D

-

T

-

V

-

A

P

A

G

Q

L

G

S

D

R

R

R

I

I

S

L

G

P

E

E

S

G

E

E

A

A

I

V

A

A

R

A

L

V

E

R

Q

A

L

A

L

L

S

E

T

K

A

A

V

V

-

A

K

Q

R

R

Q

V

C

T

L

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L
|
L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

T

G

I

V

V

A

A

A

L

C

M

A

Q

H

A

R

Q

A

A

A

S

G

Q

E

P

-

V

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

A

D

E

T

G

S

G

-

Y

-

N

N

E

E

A

F

E

R

D

E

A

A

R

R

K

A

V

V

A

V

Q

D

H

H

L

L

G

R

T

T

D

R

H

H

T

R

E

E

L

I

Y

T

V

I

D

-

A

-

R

-

T

P

A

T

M

T

E

E

V

L

P

A

K

Q

L

L

P

P

active site: A70 (≠ N79), G71 (= G80)
binding adenosine monophosphate: V235 (≠ F278), L236 (= L279), S242 (= S285), S260 (= S304), M261 (≠ I305)
binding adenosine-5'-triphosphate: V235 (≠ F278), L236 (= L279), S237 (= S280), G239 (= G282), D241 (= D284), S242 (= S285), S260 (= S304), M261 (≠ I305)
binding magnesium ion: D241 (= D284)
binding pyrophosphate 2-: S237 (= S280), G239 (= G282), D241 (= D284), S242 (= S285)

Sites not aligning to the query:

active site: 310, 336, 370, 431
binding adenosine monophosphate: 314, 318, 335, 336
binding adenosine-5'-triphosphate: 318, 335, 339, 411, 431
binding magnesium ion: 339
binding pyrophosphate 2-: 339, 411, 431

1mc1A Beta-lactam synthetase with product (dgpc), amp and ppi (see paper)
30% identity, 42% coverage: 75:347/656 of query aligns to 61:294/491 of 1mc1A

query
sites
1mc1A

T

T

I

A

T

V

F

L

N
x
A

G
|
G

E

E

I

I

Y

Y

N

N

H

R

L

D

D

E

L

L

R

L

A

S

E

V

L

L

E

P

D

-

A

A

A

G

A

P

A

A

P

P

N

E

W

-

R

-

G

-

H

-

S

G

D

D

T

A

E

E

T

L

L

V

L

L

A

R

A

L

I

L

R

E

Q

R

W

Y

G

D

L

L

V

-

P

H

A

A

L

F

Q

R

R

L

L

V

I

N

G

G

M

R

F

F

A

A

F

T

A

V

L

V

W

R

D

T

A

G

E

D

T

-

R

-

Q

R

L

V

I

L

L

L

A

A

R

T

D

D

R

H

F

A

G

G

E

S

K

V

P

P

L

L

F

Y

Y

T

G

C

W

V

S

A

G

P

A

G

D

E

L

V

V

R

F

A

G

S

S

T

E

E

L

A

K

K

A

A

L

L

A

A

H

G

P

F

G

P

W

L

A

A

P

D

S

A

L

R

D

R

R

V

A

A

A

G

L

L

T

T

D

G

F

V

M

Y

R

Q

Y

-

S

-

Y

-

V

V

P

P

A

A

P

G

A

A

T

V

I

M

W

-

R

-

D

D

V

I

R

-

K

-

L

-

P

-

A

-

S

-

F

-

V

-

A

-

F

-

V

-

A

-

D

D

A

L

G

G

P

S

G

G

T

T

S

A

P

V

T

T

A

H

Q

R

S

T

Y

W

W

-

S

-

L

-

R

-

D

-

T

-

V

-

V

T

A

P

A

G

Q

L

G

S

D

R

R

R

I

I

S

L

G

P

E

E

S

G

E

E

A

A

I

V

A

A

R

A

L

V

E

R

Q

A

L

A

L

L

S

E

T

K

A

A

V

V

-

A

K

Q

R

R

Q

V

C

T

L

P

S

G

D

D

V

T

P

P

L

L

G

-

A

V

F
x
V

L

L

S
|
S

G

G

G
|
G

V

I

D
|
D

S
|
S

S

S

T

G

I

V

V

A

A

A

L

C

M

A

Q

H

A

R

Q

A

A

A

S

G

Q

E

P

-

V

L

R

D

T

T

F

V

S
|
S

I
x
M

G

G

F

T

A

D

E

T

G

S

G

-

Y

-

N

N

E

E

A

F

E

R

D

E

A

A

R

R

K

A

V

V

A

V

Q

D

H

H

L

L

G

R

T

T

D

R

H

H

T

R

E

E

L

I

Y

T

V

I

D

-

A

-

R

-

T

P

A

T

M

T

E

E

V

L

P

A

K

Q

L

L

P

P

active site: A65 (≠ N79), G66 (= G80)
binding adenosine monophosphate: V231 (≠ F278), S233 (= S280), S238 (= S285), S256 (= S304), M257 (≠ I305)
binding magnesium ion: D237 (= D284)
binding pyrophosphate 2-: S233 (= S280), G235 (= G282), D237 (= D284), S238 (= S285)

Sites not aligning to the query:

active site: 306, 332, 366, 427
binding adenosine monophosphate: 331, 427, 430
binding magnesium ion: 335
binding deoxyguanidinoproclavaminic acid: 310, 332, 333, 336, 357, 366, 427
binding pyrophosphate 2-: 335, 407, 427, 428

Query Sequence

>WP_012045542.1 NCBI__GCF_000015165.1:WP_012045542.1
MCGIAGLFRPGGADDSALAGIVGRMTAALAHRGPDASGSWVSGRQGIALGQRRLAILDLS
EAGAQPMHSACGRYTITFNGEIYNHLDLRAELEDAAAAPNWRGHSDTETLLAAIRQWGLV
PALQRLIGMFAFALWDAETRQLILARDRFGEKPLFYGWSGADLVFGSELKALAAHPGWAP
SLDRAALTDFMRYSYVPAPATIWRDVRKLPPASFVAFVADAGPGTSPTAQSYWSLRDTVV
AAQGDRISGESEAIARLEQLLSTAVKRQCLSDVPLGAFLSGGVDSSTIVALMQAQASQPV
RTFSIGFAEGGYNEAEDARKVAQHLGTDHTELYVDARTAMEVVPKLPRIYDEPFADSSQI
PTHLVAQLARRHVTVALSGDAGDELFGGYNRHVWGSALQARLGRVPMPLRRAIGAALGAI
APEPADTLLNMLQPVLPARLRVRHAGDQVAKLARIVAADGFDGLYRTLCSIDQDPRATVV
AGEERASWAASEMKQLRGQISLLDRMTLADSLSYLSDDILQKVDRAAMAVALETRVPFLD
RDVVEFSTRVPASMKVRDGRGKWLVRQVLYRHVPADMIDRPKTGFSIPLDAWLRGPLKSW
AGDLLSPARLKRQGLFAPTRVTRMFEEHLSRRHNHAYWLWNVLMAEAWYDEWGRAG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory