SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012046899.1 NCBI__GCF_000015165.1:WP_012046899.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

4k28A 2.15 angstrom resolution crystal structure of a shikimate dehydrogenase family protein from pseudomonas putida kt2440 in complex with NAD+ (see paper)
38% identity, 91% coverage: 7:249/268 of query aligns to 2:248/266 of 4k28A

query
sites
4k28A

G

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M

binding manganese (ii) ion: C153 (≠ H158), C164 (≠ I169), V176 (≠ L181), F180 (≠ M185)
binding nicotinamide-adenine-dinucleotide: I129 (= I134), G130 (= G135), G132 (= G137), G133 (= G138), V134 (≠ A139), C153 (≠ H158), D154 (= D159), P155 (≠ S160), R159 (= R164), S193 (≠ T197), P194 (= P198), V222 (= V223), G245 (= G246), M248 (= M249)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 249

P15770 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Escherichia coli (strain K12) (see paper)
28% identity, 85% coverage: 12:240/268 of query aligns to 4:230/272 of P15770

query
sites
P15770

H

Y

I

A

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F

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N

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GAGGA 126:130 (= GAGGA 135:139) binding NADP(+)
NRTVSR 149:154 (≠ DSDAGR 159:164) binding NADP(+)
M213 (≠ V223) binding NADP(+)

Sites not aligning to the query:

237 binding NADP(+)

1nytA Shikimate dehydrogenase aroe complexed with NADP+ (see paper)
28% identity, 85% coverage: 12:240/268 of query aligns to 4:230/271 of 1nytA

query
sites
1nytA

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G

binding nadp nicotinamide-adenine-dinucleotide phosphate: K65 (= K74), D102 (= D111), G128 (= G137), G129 (= G138), A130 (= A139), N149 (≠ D159), R150 (≠ S160), T151 (≠ D161), R154 (= R164), T188 (= T197), S189 (≠ P198), S190 (≠ A199), M213 (≠ V223)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 237, 240, 241

3tozA 2.2 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with NAD.
27% identity, 91% coverage: 6:249/268 of query aligns to 10:264/291 of 3tozA

query
sites
3tozA

T

T

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T

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x
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F

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x
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M

binding nicotinamide-adenine-dinucleotide: G137 (= G135), A138 (= A136), G139 (= G137), G140 (= G138), A141 (= A139), N161 (vs. gap), R162 (vs. gap), D164 (= D159), F166 (≠ D161), T210 (= T197), G211 (≠ P198), V212 (≠ A199), M214 (= M201), F217 (vs. gap), V238 (= V223), Y240 (≠ T225), G261 (= G246), M264 (= M249)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 265

Q8Y9N5 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
27% identity, 91% coverage: 6:249/268 of query aligns to 10:264/291 of Q8Y9N5

query
sites
Q8Y9N5

T

T

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-

A

-

E

-

S

-

V

L

I

V

F

A

T

N

N

A

A

T

T

P

G

A

V

G

G

M
|
M

Q

K

P

P

-

F

-

E

G

G

D

E

P

T

L

L

P

L

V

P

D

S

M

A

A

D

T

M

V

L

S

R

P

P

S

E

A

L

Y

I

C

V

G

S

C

D

V

V

I

V

T

Y

R

K

P

P

E

T

V

K

S

T

P

R

F

L

I

L

A

E

A

I

A

A

R

E

E

E

R

Q

G

G

C

C

L

Q

T

T

A

L

T

N

G

G

T

L

D

G

M

M

SLS 26:28 (≠ VRA 22:24) binding shikimate
NRKD 161:164 (≠ --HD 158:159) binding NAD(+)
M214 (= M201) binding NADP(+)

3tnlA 1.45 angstrom crystal structure of shikimate 5-dehydrogenase from listeria monocytogenes in complex with shikimate and NAD.
27% identity, 91% coverage: 6:249/268 of query aligns to 7:261/288 of 3tnlA

query
sites
3tnlA

T

T

G

G

A

H

T

T

R

E

L

L

H

I

I

G

I

L

I

I

G

A

D

T

P

P

I

I

A

R

Q

H

V
x
S

R

L

A
x
S

P

P

A

T

G

M

V

H

S

N

R

E

A

A

F

F

A

A

E

K

R

L

G

G

S

L

D

D

A

Y

I

V

L

Y

V

L

P

A

V

F

Q

E

V

V

A

G

P

D

V

K

D

E

L

L

A

K

G

D

F

-

L

V

A

V

T

Q

A

G

T

F

R

R

V

A

K

M

N

N

L

L

D

R

G

G

I

W

V
x
N

A

V

T
x
S

I
x
M

P

P

H

N

K
|
K

F

T

A

N

C

I

Y

H

R

K

A

Y

C

L

A

D

T

K

A

L

T

S

E

P

R

A

A

A

H

E

F

L

I

V

G

G

A

A

V

V

N
|
N

L

T

M

V

R

V

R

-

S

N

P

D

N

D

G

G

G

V

W

L

H

T

G

G

D

H

M

I

V

T

D
|
D

G

G

L

T

G

G

F

Y

V

M

G

R

A

A

A

L

R

K

A

E

K

A

G

G

F

H

D

D

P

I

N

I

G

G

T

K

R

K

A

M

L

T

Q

I

I

C

G
|
G

A
|
A

G
|
G

A
|
A

G

A

S

T

A

A

I

I

A

C

L

I

A

Q

L

A

I

A

D

L

A

D

G

G

V

V

R

K

E

E

L

I

A

S

I

I

-

F

-
x
N

-
x
R

H

K

D
|
D

S

D

D
x
F

A

Y

G

A

R

N

R

A

D

E

A

K

L

T

I

V

A

E

R

K

L

I

N

N

E

S

R

K

G

T

R

D

G

C

R

K

A

A

-

Q

L

L

I

F

G

D

A

I

M

E

D

D

A

H

T

E

G

Q

F

L

D

R

-

K

-

E

-

I

-

A

-

E

-

S

-

V

L

I

V

F

A

T

N

N

A

A

T
|
T

P

G

A
x
V

G

G

M
|
M

Q

K

P

P

-
x
F

-

E

G

G

D

E

P

T

L

L

P

L

V

P

D

S

M

A

A

D

T

M

V

L

S

R

P

P

S

E

A

L

Y

I

C

V

G

S

C

D

V
|
V

I

V

T
x
Y

R

K

P

P

E

T

V

K

S

T

P

R

F

L

I

L

A

E

A

I

A

A

R

E

E

E

R

Q

G

G

C

C

L

Q

T

T

A

L

T

N

G

G

T

L

D

G

M
|
M

binding nicotinamide-adenine-dinucleotide: M71 (≠ I71), G134 (= G135), A135 (= A136), G136 (= G137), G137 (= G138), A138 (= A139), N158 (vs. gap), R159 (vs. gap), D161 (= D159), F163 (≠ D161), T207 (= T197), V209 (≠ A199), M211 (= M201), F214 (vs. gap), V235 (= V223), Y237 (≠ T225), M261 (= M249)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S23 (≠ V22), S25 (≠ A24), N68 (≠ V68), S70 (≠ T70), K74 (= K74), N95 (= N95), D110 (= D111)

Sites not aligning to the query:

Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
27% identity, 88% coverage: 14:250/268 of query aligns to 5:236/269 of Q5HNV1

query
sites
Q5HNV1

I

V

I

I

G

G

D

N

P

P

I

I

A

S

Q

H

V
x
S

R
x
L

A
x
S

P

P

-

L

-

M

-

H

A

A

G

N

V

F

S

Q

R

S

A

L

F

N

A

L

E

E

R

N

G

T

S

Y

D

E

A

A

I

I

L

N

V

V

P

P

V

V

Q

N

V

Q

A

F

P

Q

V

-

D

D

L

I

A

K

G

K

F

I

L

I

A

S

T

E

A

-

T

-

R

-

V

-

K

K

N

S

L

I

D

D

G

G

I

F

V

N

A

V

T
|
T

I

I

P

P

H

H

K

K

F

E

A

R

C

I

Y

I

R

P

A

Y

C

L

A

D

T

D

A

I

T

N

E

E

R

Q

A

A

H

K

F

S

I

V

G

G

A

A

V

V

N
|
N

L

T

M

V

R

L

R

-

S

V

P

K

N

D

G

G

G

K

W

W

H

I

G

G

D

Y

M

N

V

T

D
|
D

G

G

L

I

G

G

F

Y

V

V

G

N

A

G

A

L

R

K

A

Q

K

I

G

Y

F

E

D

G

P

I

N

E

G

D

T

A

R

Y

A

I

L

L

Q

I

I

L

G

G

A

A

G

G

A

A

G

S

S

K

A

G

I

I

A

A

L

N

A

E

L

L

I

Y

D

K

A

I

G

V

V

R

R

P

E

T

L

L

A

T

I

V

H

A

D

N

S

R

D

T

A

M

G

S

R

R

R

F

D

N

A

N

L

W

I

S

A

L

R

N

L

I

N

N

E

K

R

I

G

N

R

L

G

S

R

H

A

A

L

E

I

-

G

S

A

H

M

L

D

D

A

E

T

-

G

-

F

F

D

D

L

I

V

I

A

I

N

N

A

T

T

T

P

P

A

A

G

G

M

M

Q

N

P

G

G

N

D

T

P

D

L

S

P

V

V

I

D

S

M

L

A

N

T

R

V

L

S

A

P

S

S

H

A

T

Y

L

C

V

G

S

C

D

V

I

I

V

T
x
Y

R

N

P

P

E

Y

V

K

S

T

P

P

F

I

I

L

A

I

A

E

A

A

R

E

E

Q

R

R

G

G

C

N

L

P

T

I

A

Y

T

N

G

G

T

L

D

D

M

M

Y

F

SLS 13:15 (≠ VRA 22:24) binding shikimate
T60 (= T70) binding shikimate
N85 (= N95) binding shikimate
D100 (= D111) binding shikimate
Y211 (≠ T225) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.

Sites not aligning to the query:

239 binding shikimate

Q9KVT3 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
26% identity, 87% coverage: 12:244/268 of query aligns to 8:238/278 of Q9KVT3

query
sites
Q9KVT3

H

Y

I

A

I

V

I

F

G

G

D

N

P

P

I

I

A

N

Q

H

V
x
S

R
x
K

A
x
S

P

P

A

F

G

-

V

I

S

H

R

T

A

L

F

F

A

A

E

R

R

Q

G

T

S

Q

D

Q

A

S

I

M

L

I

V

Y

P

T

V

A

Q

Q

V

C

A

V

P

P

V

V

D

D

L

-

A

-

G

G

F

F

L

T

A

E

T

A

A

A

T

K

R

H

V

F

-

F

-

A

K

Q

N

G

L

G

D

R

G

G

I

C

V

N

A

V

T

T

I

V

P

P

H

F

K

K

F

E

A

E

C

A

Y

Y

R

R

A

F

C

A

A

D

T

R

A

L

T

T

E

E

R

R

A

A

H

R

F

L

I

A

G

G

A

A

V

V

N
|
N

L

T

M

L

R

K

S

L

P

D

N

D

G

G

G

E

W

I

H

L

G

G

D

D

M

N

V

T

D
|
D

G

G

L

E

G

G

F

L

V

V

G

Q

A

D

A

L

R

L

A

A

K

Q

G

Q

F

V

D

L

P

L

N

K

G

G

T

A

R

T

A

I

L

L

Q

L

I

I

G

G

A

A

G

G

A

A

G

A

S

R

A

G

I

V

A

L

L

K

A

P

L

L

I

L

D

D

A

Q

G

Q

V

P

R

A

E

S

L

I

A

T

I

V

H

T

D
x
N

S
x
R

D
x
T

A
x
F

G
x
A

R
x
K

R

A

D

E

A

Q

L

L

I

A

A

E

R

L

L

V

N

A

E

A

R

Y

G

G

R

E

G

V

R

K

A

A

L

Q

I

A

G

F

A

E

M

Q

D

L

A

K

T

Q

G

S

F

Y

D

D

L

V

V

I

A

I

N

N

A

S

T

T

P

S

A

A

G

S

M

L

Q

-

P

D

G

G

D

E

P

L

L

P

P

A

V

I

D

D

M

P

A

V

T

I

V

F

S

S

P

S

S

R

A

S

Y

V

C

C

G

Y

C

D

V

M

I

M

T

Y

R

G

P

K

E

G

V

Y

S

T

P

V

F

F

I

N

A

Q

A

W

A

A

R

R

E

Q

R

H

G

G

C

C

L

A

T

Q

A

A

SKS 18:20 (≠ VRA 22:24) binding shikimate
N90 (= N95) binding shikimate
D106 (= D111) binding shikimate
NRTFAK 154:159 (≠ DSDAGR 159:164) binding NADP(+)

Sites not aligning to the query:

248 binding shikimate

3pgjA 2.49 angstrom resolution crystal structure of shikimate 5- dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate
26% identity, 87% coverage: 12:244/268 of query aligns to 4:234/272 of 3pgjA

query
sites
3pgjA

H

Y

I

A

I

V

I

F

G

G

D

N

P

P

I

I

A

N

Q

H

V
x
S

R

K

A
x
S

P

P

A

F

G

-

V

I

S

H

R

T

A

L

F

F

A

A

E

R

R

Q

G

T

S

Q

D

Q

A

S

I

M

L

I

V

Y

P

T

V

A

Q

Q

V

C

A

V

P

P

V

V

D

D

L

-

A

-

G

G

F

F

L

T

A

E

T

A

A

A

T

K

R

H

V

F

-

F

-

A

K

Q

N

G

L

G

D

R

G

G

I

C

V
x
N

A

V

T
|
T

I

V

P

P

H

F

K
|
K

F

E

A

E

C

A

Y

Y

R

R

A

F

C

A

A

D

T

R

A

L

T

T

E

E

R

R

A

A

H

R

F

L

I

A

G

G

A

A

V

V

N
|
N

L

T

M

L

R

K

S

L

P

D

N

D

G

G

G

E

W

I

H

L

G

G

D

D

M

N

V

T

D
|
D

G

G

L

E

G

G

F

L

V

V

G

Q

A

D

A

L

R

L

A

A

K

Q

G

Q

F

V

D

L

P

L

N

K

G

G

T

A

R

T

A

I

L

L

Q

L

I

I

G

G

A

A

G

G

A

A

G

A

S

R

A

G

I

V

A

L

L

K

A

P

L

L

I

L

D

D

A

Q

G

Q

V

P

R

A

E

S

L

I

A

T

I

V

H

T

D

N

S

R

D

T

A

F

G

A

R

K

R

A

D

E

A

Q

L

L

I

A

A

E

R

L

L

V

N

A

E

A

R

Y

G

G

R

E

G

V

R

K

A

A

L

Q

I

A

G

F

A

E

M

Q

D

L

A

K

T

Q

G

S

F

Y

D

D

L

V

V

I

A

I

N

N

A

S

T

T

P

S

A

A

G

S

M

L

Q

-

P

D

G

G

D

E

P

L

L

P

P

A

V

I

D

D

M

P

A

V

T

I

V

F

S

S

P

S

S

R

A

S

Y

V

C

C

G

Y

C

D

V

M

I

M

T

Y

R

G

P

K

E

G

V

Y

S

T

P

V

F

F

I

N

A

Q

A

W

A

A

R

R

E

Q

R

H

G

G

C

C

L

A

T

Q

A

A

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (≠ V22), S16 (≠ A24), N59 (≠ V68), T61 (= T70), K65 (= K74), N86 (= N95), D102 (= D111)

Sites not aligning to the query:

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 244

2gptA Crystal structure of arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate (see paper)
26% identity, 90% coverage: 9:250/268 of query aligns to 234:461/498 of 2gptA

query
sites
2gptA

T

T

R

K

L

V

H

Y

I

G

I
|
I

I

I

G

G

D

K

P

P

I

V

A

S

Q

H

V
x
S

R

K

A
x
S

P

P

A

I

G

V

V

H

S

N

R

Q

A

A

F

F

A

K

E

S

R

V

G

D

S

F

D

N

A

G

I

V

L

Y

V

V

P

H

V

L

Q

L

V

V

A

D

P

-

V

-

D

N

L

L

A

V

G

S

F

F

L

L

A

Q

T

-

A

A

T

Y

R

S

V

S

K

S

N

D

L

F

D

A

G

G

I

F

V

S

A

C

T
|
T

I

I

P

P

H

H

K
|
K

F

E

A

A

C

A

Y

L

R

Q

A

C

C

C

A

D

T

E

A

V

T

D

E

P

R

L

A

A

H

K

F

S

I

I

G

G

A

A

V

V

N
|
N

-

T

L

I

M

L

R

R

S

K

P

S

N

D

G

G

G

K

W

L

H

L

G

G

D

Y

M

N

V

T

D
|
D

G

C

L

I

G

G

F

S

V

I

G

S

A

A

A

I

R

E

A

-

K

-

G

-

F

-

D

-

P

-

N

-

G

-

T

-

R

-

A

-

L

-

Q

-

I

-

G

-

A

-

G

-

A

D

G

G

S

L

A

T

I

V

A

V

L

V

A

I

L

G

I

A

D

G

A

G

G

A

V

G

R

K

E

A

L

L

A

A

I

Y

H

G

D

A

S

K

D

E

A

K

G

G

R

A

R

K

D

V

A

V

L

I

I

A

A

N

R

R

L

T

N

Y

E

E

R

R

G

E

R

L

G

A

R

E

A

A

L

-

I

I

G

G

A

A

M

L

D

S

A

L

T

T

G

D

F

L

D

D

-

N

-

Y

-

E

-

D

-

G

-

M

L

V

V

L

A

A

N

N

A

T

T

T

P

S

A

M

G

G

M

M

Q

Q

P

P

G

N

-

V

D

E

P

E

L

T

P

P

V

I

D

S

M

K

A

D

T

A

V

L

S

K

P

H

S

Y

A

A

Y

L

C

V

G

F

C

D

V

A

I

V

T
x
Y

R

T

P

P

E

R

V

I

S

T

P

R

F

L

I

L

A

R

A

E

A

A

R

E

E

E

R

S

G

G

C

A

L

I

T

T

A

V

T

S

G

G

T

S

D

E

M

M

Y

F

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: I239 (= I14), S247 (≠ V22), S249 (≠ A24), T292 (= T70), K296 (= K74), N317 (= N95), D334 (= D111), Y436 (≠ T225)

Sites not aligning to the query:

active site: 70, 125, 152
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 464, 468

3dooA Crystal structure of shikimate dehydrogenase from staphylococcus epidermidis complexed with shikimate (see paper)
25% identity, 88% coverage: 14:250/268 of query aligns to 5:227/258 of 3dooA

query
sites
3dooA

I

V

I

I

G

G

D

N

P

P

I

I

A

S

Q

H

V
x
S

R

L

A
x
S

P

P

-

L

-

M

-

H

A

A

G

N

V

F

S

Q

R

S

A

L

F

N

A

L

E

E

R

N

G

T

S

Y

D

E

A

A

I

I

L

N

V

V

P

P

V

V

Q

N

V

Q

A

F

P

Q

V

-

D

D

L

I

A

K

G

K

F

I

L

I

A

S

T

E

A

-

T

-

R

-

V

-

K

K

N

S

L

I

D

D

G

G

I

F

V
x
N

A

V

T
|
T

I

I

P

P

H

H

K
|
K

F

E

A

R

C

I

Y

I

R

P

A

Y

C

L

A

D

T

D

A

I

T

N

E

E

R

Q

A

A

H

K

F

S

I

V

G

G

A

A

V

V

N
|
N

L

T

M

V

R

-

R

L

S

V

P

K

N

D

G

G

G

K

W

W

H

I

G

G

D

Y

M

N

V

T

D
|
D

G

G

L

I

G

G

F

Y

V

V

G

N

A

G

A

L

R

K

A

Q

K

I

G

Y

F

E

D

G

P

I

N

E

G

D

T

A

R

Y

A

I

L

L

Q

I

I

L

G

G

A

A

G

G

A

A

G

S

S

K

A

G

I

I

A

A

L

N

A

E

L

L

I

Y

D

K

A

I

G

V

V

R

R

P

E

T

L

L

A

T

I

V

H

A

D

N

S

R

D

T

A

M

G

S

R

R

R

F

D

N

A

N

L

W

I

S

A

L

R

N

L

I

N

N

E

K

R

I

G

N

R

L

G

S

R

H

A

A

L

E

I

-

G

S

A

H

M

L

D

D

A

E

T

-

G

-

F

F

D

D

L

I

V

I

A

I

N

N

A

T

T

T

P

P

A

V

G

-

M

-

Q

-

P

-

G

-

D

-

P

-

L

-

P

-

V

I

D

S

M

L

A

N

T

R

V

L

S

A

P

S

S

H

A

T

Y

L

C

V

G

S

C

D

V

I

I

V

T

Y

R

N

P

P

E

Y

V

K

S

T

P

P

F

I

I

L

A

I

A

E

A

A

R

E

E

Q

R

R

G

G

C

N

L

P

T

I

A

Y

T

N

G

G

T

L

D

D

M

M

Y
x
F

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S13 (≠ V22), S15 (≠ A24), N58 (≠ V68), T60 (= T70), K64 (= K74), N85 (= N95), D100 (= D111), F227 (≠ Y250)

Sites not aligning to the query:

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 230

6bmqA Crystal structure of arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase (t381g mutant) in complex with tartrate and shikimate (see paper)
25% identity, 90% coverage: 9:250/268 of query aligns to 234:461/498 of 6bmqA

query
sites
6bmqA

T

T

R

K

L

V

H

Y

I

G

I

I

G

G

D

K

P

P

I

V

A

S

Q

H

V
x
S

R

K

A
x
S

P

P

A

I

G

V

V

H

S

N

R

Q

A

A

F

F

A

K

E

S

R

V

G

D

S

F

D

N

A

G

I

V

L

Y

V

V

P

H

V

L

Q

L

V

V

A

D

P

-

V

-

D

N

L

L

A

V

G

S

F

F

L

L

A

Q

T

-

A

A

T

Y

R

S

V

S

K

S

N

D

L

F

D

A

G

G

I

F

V

S

A
x
C

T

G

I

I

P

P

H

H

K
|
K

F

E

A

A

C

A

Y

L

R

Q

A

C

C

C

A

D

T

E

A

V

T

D

E

P

R

L

A

A

H

K

F

S

I

I

G

G

A

A

V

V

N
|
N

-

T

L

I

M

L

R

R

S

K

P

S

N

D

G

G

G

K

W

L

H

L

G

G

D

Y

M

N

V

T

D
|
D

G

C

L

I

G

G

F

S

V

I

G

S

A

A

A

I

R

E

A

-

K

-

G

-

F

-

D

-

P

-

N

-

G

-

T

-

R

-

A

-

L

-

Q

-

I

-

G

-

A

-

G

-

A

D

G

G

S

L

A

T

I

V

A

V

L

V

A

I

L

G

I

A

D

G

A

G

G

A

V

G

R

K

E

A

L

L

A

A

I

Y

H

G

D

A

S

K

D

E

A

K

G

G

R

A

R

K

D

V

A

V

L

I

I

A

A

N

R

R

L

T

N

Y

E

E

R

R

G

E

R

L

G

A

R

E

A

A

L

-

I

I

G

G

A

A

M

L

D

S

A

L

T

T

G

D

F

L

D

D

-

N

-

Y

-

E

-

D

-

G

-

M

L

V

V

L

A

A

N

N

A

T

T

T

P

S

A

M

G

G

M

M

Q

Q

P

P

G

N

-

V

D

E

P

E

L

T

P

P

V

I

D

S

M

K

A

D

T

A

V

L

S

K

P

H

S

Y

A

A

Y

L

C

V

G

F

C

D

V

A

I

V

T
x
Y

R

T

P

P

E

R

V

I

S

T

P

R

F

L

I

L

A

R

A

E

A

A

R

E

E

E

R

S

G

G

C

A

L

I

T

T

A

V

T

S

G

G

T

S

D

E

M

M

Y

F

binding (1S,3R,4S,5R)-1,3,4,5-tetrahydroxycyclohexanecarboxylic acid: S247 (≠ V22), S249 (≠ A24), C291 (≠ A69), K296 (= K74), N317 (= N95), D334 (= D111), Y436 (≠ T225)

Sites not aligning to the query:

active site: 70, 125, 152
binding (1S,3R,4S,5R)-1,3,4,5-tetrahydroxycyclohexanecarboxylic acid: 464

3sefA 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
25% identity, 87% coverage: 12:244/268 of query aligns to 4:230/268 of 3sefA

query
sites
3sefA

H

Y

I

A

I

V

I

F

G

G

D

N

P

P

I

-

A

-

Q

-

V

S

R

K

A

S

P

P

A

F

G

-

V

I

S

H

R

T

A

L

F

F

A

A

E

R

R

Q

G

T

S

Q

D

Q

A

S

I

M

L

I

V

Y

P

T

V

A

Q

Q

V

C

A

V

P

P

V

-

D

-

L

-

A

D

G

G

F

F

L

T

A

E

T

A

A

A

T

K

R

H

V

F

-

F

-

A

K

Q

N

G

L

G

D

R

G

G

I

C

V

N

A

V

T

T

I

V

P

P

H

F

K

K

F

E

A

E

C

A

Y

Y

R

R

A

F

C

A

A

D

T

R

A

L

T

T

E

E

R

R

A

A

H

R

F

L

I

A

G

G

A

A

V

V

N

N

L

T

M

L

R

K

S

L

P

D

N

D

G

G

G

E

W

I

H

L

G

G

D

D

M

N

V

T

D

D

G

G

L

E

G

G

F

L

V

V

G

Q

A

D

A

L

R

L

A

A

K

Q

G

Q

F

V

D

L

P

L

N

K

G

G

T

A

R

T

A

I

L

L

Q

L

I

I

G

G

A

A

G
|
G

G

G

A
|
A

G

A

S

R

A

G

I

V

A

L

L

K

A

P

L

L

I

L

D

D

A

Q

G

Q

V

P

R

A

E

S

L

I

A

T

I

V

H

T

D
x
N

S
x
R

D
x
T

A

F

G

A

R

K

R

A

D

E

A

Q

L

L

I

A

A

E

R

L

L

V

N

A

E

A

R

Y

G

G

R

E

G

V

R

K

A

A

L

Q

I

A

G

F

A

E

M

Q

D

L

A

K

T

Q

G

S

F

Y

D

D

L

V

V

I

A

I

N

N

A
x
S

T
|
T

P
x
S

A
|
A

G

S

M

L

Q

-

P

D

G

G

D

E

P

L

L

P

P

A

V

I

D

D

M

P

A

V

T

I

V

F

S

S

P

S

S

R

A

S

Y

V

C

C

G

Y

C

D

V

M

I
x
M

T

Y

R

G

P

K

E

G

V

Y

S

T

P

V

F

F

I

N

A

Q

A

W

A

A

R

R

E

Q

R

H

G

G

C

C

L

A

T

Q

A

A

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G124 (= G137), A126 (= A139), N146 (≠ D159), R147 (≠ S160), T148 (≠ D161), S183 (≠ A196), T184 (= T197), S185 (≠ P198), A186 (= A199), M210 (≠ I224)

3sefC 2.4 angstrom resolution crystal structure of shikimate 5-dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate and NADPH
27% identity, 70% coverage: 12:199/268 of query aligns to 4:189/244 of 3sefC

query
sites
3sefC

H

Y

I

A

I

V

I

F

G

G

D

N

P

P

I

I

A

N

Q

H

V
x
S

R

K

A
x
S

P

P

A

F

G

-

V

I

S

H

R

T

A

L

F

F

A

A

E

R

R

Q

G

T

S

Q

D

Q

A

S

I

M

L

I

V

Y

P

T

V

A

Q

Q

V

C

A

V

P

P

V

V

D

D

L

-

A

-

G

G

F

F

L

T

A

E

T

A

A

A

T

K

R

H

V

F

-

F

-

A

K

Q

N

G

L

G

D

R

G

G

I

C

V

N

A

V

T

T

I

V

P

P

H

F

K
|
K

F

E

A

E

C

A

Y

Y

R

R

A

F

C

A

A

D

T

R

A

L

T

T

E

E

R

R

A

A

H

R

F

L

I

A

G

G

A

A

V

V

N

N

L

T

M

L

R

K

S

D

P

-

N

D

G

G

G

E

W

I

H

L

G

G

D

D

M

N

V

T

D
|
D

G

G

L

E

G

G

F

L

V

V

G

Q

A

D

A

L

R

L

A

A

K

Q

G

Q

F

V

D

L

P

L

N

K

G

G

T

A

R

T

A

I

L

L

Q

L

I

I

G

G

A

A

G

G

A

A

G

A

S

R

A

G

I

V

A

L

L

K

A

P

L

L

I

L

D

D

A

Q

G

Q

V

P

R

A

E

S

L

I

A

T

I

V

H

T

D

N

S

R

D

T

A

F

G

A

R

K

R

A

D

E

A

Q

L

L

I

A

A

E

R

L

L

V

N

A

E

A

R

Y

G

G

R

E

G

V

R

K

A

A

L

Q

I

A

G

F

A

E

M

Q

D

L

A

K

T

Q

G

S

F

Y

D

D

L

V

V

I

A

I

N

N

A

S

T

T

P

S

A

A

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (≠ V22), S16 (≠ A24), K65 (= K74), D101 (= D111)

Sites not aligning to the query:

binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: 217

2o7sA Crystal structure of the a. Thaliana dhq-dehydroshikimate-sdh- shikimate-NADP(h)
28% identity, 90% coverage: 9:250/268 of query aligns to 234:463/500 of 2o7sA

query
sites
2o7sA

T

T

R

K

L

V

H

Y

I

G

I
|
I

I

I

G

G

D

K

P

P

I

V

A

S

Q

H

V
x
S

R

K

A
x
S

P

P

A

I

G

V

V

H

S

N

R

Q

A

A

F

F

A

K

E

S

R

V

G

D

S

F

D

N

A

G

I

V

L

Y

V

V

P

H

V

L

Q

L

V

V

A

D

P

-

V

-

D

N

L

L

A

V

G

S

F

F

L

L

A

Q

T

-

A

A

T

Y

R

S

V

S

K

S

N

D

L

F

D

A

G

G

I

F

V

S

A

C

T
|
T

I
|
I

P
|
P

H

H

K
|
K

F

E

A

A

C

A

Y

L

R

Q

A

C

C

C

A

D

T

E

A

V

T

D

E

P

R

L

A

A

H

K

F

S

I

I

G

G

A

A

V

V

N
|
N

-

T

L

I

M

L

R

R

S

K

P

S

N

D

G

G

G

K

W

L

H

L

G

G

D

Y

M

N

V

T

D
|
D

G

C

L

I

G

G

F

S

V

I

G

S

A

A

A

I

R

E

A

-

K

-

G

-

F

-

D

-

P

-

N

D

G

G

T

L

R

T

A

V

L

V

Q

V

I

I

G

G

A

A

G
|
G

A
|
A

G

G

S

K

A

A

I

L

A

A

L

Y

A

G

L

A

I

K

D

E

A

K

G

G

V

A

R

V

E

V

L

I

A

A

I

-

H

-

D

-

S

-

D

-

A

-

G

-

R

-

D

-

A

-

L

-

I

-

A
x
N

R
|
R

L
x
T

N

Y

E

E

R
|
R

G

A

-

L

-

E

R

L

G

A

R

E

A

A

L

I

I

G

G

G

A

A

M

L

D

S

A

L

T

T

G

D

F

L

D

D

-

N

-

Y

-

E

-

D

-

G

-

M

L

V

V

L

A

A

N

N

A

T

T
|
T

P
x
S

A
x
M

G

G

M

M

Q

Q

P

P

G

N

-

V

D

E

P

E

L

T

P

P

V

I

D

S

M

K

A

D

T

A

V

L

S

K

P

H

S

Y

A

A

Y

L

C

V

G

F

C

D

V
x
A

I

V

T
x
Y

R

T

P

P

E

R

V

I

S

T

P

R

F

L

I

L

A

R

A

E

A

A

R

E

E

E

R

S

G

G

C

A

L

I

T

T

A

V

T

S

G

G

T

S

D

E

M
|
M

Y
x
F

binding 3-dehydroshikimate: I239 (= I14), S247 (≠ V22), S249 (≠ A24), T292 (= T70), K296 (= K74), N317 (= N95), D334 (= D111), Y438 (≠ T225)
binding nadp nicotinamide-adenine-dinucleotide phosphate: I293 (= I71), P294 (= P72), K296 (= K74), D334 (= D111), G354 (= G137), G355 (= G138), A356 (= A139), N374 (≠ A170), R375 (= R171), T376 (≠ L172), R379 (= R175), T409 (= T197), S410 (≠ P198), M411 (≠ A199), A436 (≠ V223), M462 (= M249), F463 (≠ Y250)

Sites not aligning to the query:

active site: 70, 125, 152
binding 3-dehydroshikimate: 35, 152, 190, 202, 211, 212, 215, 466, 470

7colA Crystal structure of 5-ketofructose reductase complexed with NADPH (see paper)
30% identity, 94% coverage: 18:268/268 of query aligns to 14:267/280 of 7colA

query
sites
7colA

P

P

I

C

A

A

Q

D

V

N

R

P

A

T

P

V

A

A

G

M

V

V

S

E

R

A

A

G

F

Y

A

H

E

H

R

A

G

G

S

L

D

D

A

A

I

R

L

Y

V

I

P

N

V

C

Q

D

V

V

A

K

P

A

V

S

D

G

L

L

A

A

G

D

F

A

L

V

A

K

T

G

A

A

T

K

R

A

V

M

K

E

N

W

L

V

D

-

G

G

I

F

V

N

A

C

T

S

I

L

P

P

H

H

K

K

F

V

A

A

C

V

Y

L

R

D

A

H

C

L

A

D

T

D

A

I

T

A

E

E

R

S

A

A

H

R

F

L

I

I

G

G

A

A

V

V

N

N

L

C

M

V

R

A

R

I

S

R

P

-

N

E

G

G

G

K

W

L

H

I

G

G

D

H

M

N

V

T

D

D

G

G

L

K

G

G

F

F

V

L

G

A

A

S

A

L

R

K

A

T

K

V

G

-

F

T

D

S

P

P

N

A

G

G

T

K

R

R

A

V

L

V

Q

I

I

L

G
|
G

A

A

G
|
G

A
|
A

G

A

S

R

A

A

I

I

A

A

L

V

A

E

L

L

I

A

D

L

A

A

G

G

V

A

R

A

E

H

L

I

A

T

I

I

H

V

D
x
N

S
x
R

D

D

A

A

G

S

R
x
K

R

A

D

E

A

T

L

I

I

A

A

A

R

L

L

I

N

N

E

D

R

K

-

T

-

E

-

A

-

T

G

G

R

E

G

A

R

Q

A

A

L

W

I

S

G

G

A

K

M

F

D

S

A

L

-

P

T

T

G

G

F

T

D

D

L

I

V

L

A

I

N

N

A

A

T
|
T

P
x
S

A
x
I

G

G

M

L

Q

-

P

-

G

G

D

D

P

P

L

N

-

A

-

P

P

P

V

V

D

E

M

M

A

G

T

S

V

L

S

T

P

K

S

E

A

T

Y

V

C

V

G

A

C

D

V
|
V

I

I

T

P

R

N

P

P

E

P

V

Q

S

T

P

R

F

F

I

L

A

K

A

D

A

A

R

K

E

A

R

L

G

G

C

C

L

T

T

T

A

L

T

D

G

G

T

L

D

G

M

M

Y

L

R

V

A

N

L
x
Q

E

G

R

V

V

I

M

G

V

V

D

E

F

I

L

W

L

L

S

G

R

R

Q

T

P

L

P

D

S

S

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G128 (= G135), G130 (= G137), G131 (= G138), A132 (= A139), N152 (≠ D159), R153 (≠ S160), K157 (≠ R164), T195 (= T197), S196 (≠ P198), I197 (≠ A199), V222 (= V223), Q252 (≠ L253)

P43876 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) (see paper)
25% identity, 94% coverage: 11:261/268 of query aligns to 3:261/272 of P43876

query
sites
P43876

L

L

H

Y

I

A

I

V

I

W

G

G

D

N

P

P

I

I

A

A

Q

Q

V

S

R

K

A

S

P

P

A

L

G

-

V

I

S

Q

R

N

A

K

F

L

A

A

E

A

R

Q

G

T

S

H

D

Q

A

T

I

M

L

E

V

Y

P

I

V

A

Q

K

V

L

A

G

P

D

V

L

D

D

L

A

A

F

G

E

F

Q

L

Q

A

L

T

L

A

A

T

F

R

F

V

E

K

E

N

G

L

A

D

K

G

G

I

C

V

N

A

I

T

T

I

S

P

P

H

F

K

K

F

E

A

R

C

A

Y

Y

R

Q

A

L

C

A

A

D

T

E

A

Y

T

S

E

Q

R

R

A

A

H

K

F

L

I

A

G

E

A

A

V

C

N

N

L

T

M

L

R

K

S

L

P

D

N

D

G

G

G

K

W

L

H

Y

G

A

D

D

M

N

V

T

D

D

G

G

L

I

G

G

F

L

V

V

-

T

G

D

A

L

A

Q

R

R

A

L

K

N

G

W

F

L

D

R

P

P

N

N

G

-

T

Q

R

H

A

V

L

L

Q

I

I

L

G
|
G

A
|
A

G
|
G

A
|
A

G

T

S

K

A

G

I

V

A

L

L

L

A

P

L

L

I

L

D

Q

A

A

G

Q

V

-

R

Q

E

N

L

I

A

V

I

L

H

A

D
x
N

S
x
R

D
x
T

A
x
F

G
x
S

R
x
K

R

T

D

K

A

E

L

L

I

A

A

E

R

R

L

F

N

Q

E

P

R

Y

G

G

R

N

G

I

R

Q

A

A

L

V

I

-

G

-

A

S

M

M

D

D

A

S

-

I

-

P

-

L

T

Q

G

T

F

Y

D

D

L

L

V

V

A

I

N

N

A

A

T

T

P
x
S

A

A

G

G

M

L

Q

S

P

G

G

G

D

T

P

A

L

-

P

S

V

V

D

D

M

A

A

E

T

I

V

L

S

K

P

L

-

G

S

S

A

A

Y

F

C

Y

G

D

C

M

V

Q

I

Y

T

A

R

K

P

G

E

T

V

D

S

T

P

P

F

F

I

I

A

A

A

L

A

C

R

K

E

S

R

L

G

G

C

L

-

T

-

N

-

V

-

S

-

D

-

G

-

F

-

G

-

M

L

L

T

V

A

A

T

Q

G

A

T

A

D

H

M

S

Y

F

R

H

A

L

L

W

E

R

R

G

V

V

M

M

V

P

D

D

F

F

L

V

GAGGA 126:130 (= GAGGA 135:139) binding NADP(+)
NRTFSK 149:154 (≠ DSDAGR 159:164) binding NADP(+)
S189 (≠ P198) binding NADP(+)

Q9X5C9 Quinate/shikimate dehydrogenase (NAD(+)); QSDH; EC 1.1.1.-; EC 1.1.1.24 from Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / BCRC 11384 / CCUG 27702 / LMG 3730 / NBRC 12168 / NCIMB 10025 / NRRL B-2784 / 534) (see paper)
34% identity, 69% coverage: 64:249/268 of query aligns to 63:254/283 of Q9X5C9

query
sites
Q9X5C9

L

F

D

N

G

G

I

L

V

N

A

I

T
|
T

I

H

P

P

H

Y

K
|
K

F

Q

A

A

C

V

Y

L

R

P

A

L

C

L

A

D

T

E

A

V

T

S

E

E

R

Q

A

A

H

T

F

Q

I

L

G

G

A

A

V

V

N
|
N

L

T

M

V

R

V

R

I

S

D

P

A

N

T

G

G

G

H

W

T

H

T

G

G

D

H

M

N

V

T

D
|
D

G

V

L

S

G

G

F

F

V

-

G

G

A

R

A

G

R

M

A

E

K

E

G

G

F

L

D

-

P

P

N

N

G

A

T

K

-

L

-

D

R

S

A

V

L

V

Q

Q

I

V

G

G

A

A

G

G

G
|
G

A
x
V

G

G

S

N

A

A

I

V

A

A

L

Y

A

A

L

L

I

V

D

T

A

H

G

G

V

V

R

Q

E

K

L

L

A

Q

I

V

H

A

D
|
D

S

L

D

D

A

T

G

S

R
|
R

R

A

D

Q

A

A

L

L

I

A

A

D

R

V

L

I

N

N

E

-

R

N

G

A

R

V

G

G

R

R

A

E

L

A

I

V

G

V

A

G

M

V

D

D

A

A

T

R

G

G

F

I

-

E

-

D

-

V

-

I

-

A

D

D

L

G

V

V

A

V

N

N

A

A

T

T

P
|
P

A
x
M

G
|
G

M
|
M

Q

-

P

P

G

A

D

H

P

P

-

G

L

T

P
x
A

V

F

D

D

M

V

A

S

T

C

V

L

S

T

P

K

S

D

A

H

Y

W

C

V

G

G

C

D

V
|
V

I

V

T

Y

R

M

P

P

E

I

V

E

S

T

P

E

F

L

I

L

A

K

A

A

R

R

E

A

R

L

G

G

C

C

L

E

T

T

A

L

T

D

G
|
G

T

T

D

R

M

M

T69 (= T70) binding L-quinate; binding shikimate
K73 (= K74) active site, Proton acceptor; binding L-quinate; binding shikimate
N94 (= N95) binding L-quinate; binding shikimate
D110 (= D111) binding L-quinate; binding shikimate
GV 137:138 (≠ GA 138:139) binding NAD(+)
D158 (= D159) binding NAD(+)
R163 (= R164) binding NAD(+)
PMGM 203:206 (≠ PAGM 198:201) binding NAD(+)
A213 (≠ P208) binding NAD(+)
V228 (= V223) binding NAD(+)
G251 (= G246) binding NAD(+)

Sites not aligning to the query:

17 binding shikimate
17:19 binding L-quinate
258 binding L-quinate; binding shikimate

3jyqA Quinate dehydrogenase from corynebacterium glutamicum in complex with shikimate and nadh (see paper)
34% identity, 69% coverage: 64:249/268 of query aligns to 62:253/282 of 3jyqA

query
sites
3jyqA

L

F

D

N

G

G

I

L

V
x
N

A

I

T
|
T

I

H

P

P

H

Y

K

K

F

Q

A

A

C

V

Y

L

R

P

A

L

C

L

A

D

T

E

A

V

T

S

E

E

R

Q

A

A

H

T

F

Q

I

L

G

G

A

A

V

V

N
|
N

L

T

M

V

R

V

R

I

S

D

P

A

N

T

G

G

G

H

W

T

H

T

G

G

D

H

M

N

V

T

D
|
D

G

V

L

S

G

G

F

F

V

-

G

G

A

R

A

G

R

M

A

E

K

E

G

G

F

L

D

-

P

P

N

N

G

A

T

K

-

L

-

D

R

S

A

V

L

V

Q

Q

I
x
V

G

G

A

A

G
|
G

A
x
V

G

G

S

N

A

A

I

V

A

A

L

Y

A

A

L

L

I

V

D

T

A

H

G

G

V

V

R

Q

E

K

L

L

A

Q

I

V

H

A

D
|
D

S
x
L

D

D

A

T

G

S

R
|
R

R

A

D

Q

A

A

L

L

I

A

A

D

R

V

L

I

N

N

E

-

R

N

G

A

R

V

G

G

R

R

A

E

L

A

I

V

G

V

A

G

M

V

D

D

A

A

T

R

G

G

F

I

-

E

-

D

-

V

-

I

-

A

D

D

L

G

V

V

A

V

N

N

A

A

T
|
T

P
|
P

A

M

G

G

M
|
M

Q

-

P

P

G

A

D

H

P

P

-

G

L

T

P

A

V

F

D

D

M

V

A

S

T

C

V

L

S

T

P

K

S

D

A

H

Y

W

C

V

G

G

C

D

V
|
V

I

V

T

Y

R

M

P

P

E

I

V

E

S

T

P

E

F

L

I

L

A

K

A

A

R

R

E

A

R

L

G

G

C

C

L

E

T

T

A

L

T

D

G

G

T

T

D

R

M

M

binding nicotinamide-adenine-dinucleotide: V132 (≠ I134), G135 (= G137), G136 (= G138), V137 (≠ A139), D157 (= D159), L158 (≠ S160), R162 (= R164), T201 (= T197), P202 (= P198), M205 (= M201), V227 (= V223)
binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: N66 (≠ V68), T68 (= T70), N93 (= N95), D109 (= D111)

Sites not aligning to the query:

3jypA Quinate dehydrogenase from corynebacterium glutamicum in complex with quinate and nadh (see paper)
34% identity, 69% coverage: 64:249/268 of query aligns to 62:253/282 of 3jypA

query
sites
3jypA

L

F

D

N

G

G

I

L

V
x
N

A

I

T
|
T

I

H

P

P

H

Y

K
|
K

F

Q

A

A

C

V

Y

L

R

P

A

L

C

L

A

D

T

E

A

V

T

S

E

E

R

Q

A

A

H

T

F

Q

I

L

G

G

A

A

V

V

N
|
N

L

T

M

V

R

V

R

I

S

D

P

A

N

T

G

G

G

H

W

T

H

T

G

G

D

H

M

N

V

T

D
|
D

G

V

L

S

G

G

F

F

V

-

G

G

A

R

A

G

R

M

A

E

K

E

G

G

F

L

D

-

P

P

N

N

G

A

T

K

-

L

-

D

R

S

A

V

L

V

Q

Q

I
x
V

G

G

A

A

G
|
G

G

G

A
x
V

G

G

S

N

A

A

I

V

A

A

L

Y

A

A

L

L

I

V

D

T

A

H

G

G

V

V

R

Q

E

K

L

L

A

Q

I

V

H

A

D
|
D

S
x
L

D

D

A

T

G

S

R
|
R

R

A

D

Q

A

A

L

L

I

A

A

D

R

V

L

I

N

N

E

-

R

N

G

A

R

V

G

G

R

R

A

E

L

A

I

V

G

V

A

G

M

V

D

D

A

A

T

R

G

G

F

I

-

E

-

D

-

V

-

I

-

A

D

D

L

G

V

V

A

V

N

N

A

A

T
|
T

P
|
P

A

M

G

G

M
|
M

Q

-

P

P

G

A

D

H

P

P

-

G

L

T

P
x
A

V

F

D

D

M

V

A

S

T

C

V

L

S

T

P

K

S

D

A

H

Y

W

C

V

G

G

C

D

V
|
V

I

V

T
x
Y

R

M

P

P

E

I

V

E

S

T

P

E

F

L

I

L

A

K

A

A

R

R

E

A

R

L

G

G

C

C

L

E

T

T

A

L

T

D

G

G

T

T

D

R

M

M

binding nicotinamide-adenine-dinucleotide: V132 (≠ I134), G135 (= G137), V137 (≠ A139), D157 (= D159), L158 (≠ S160), R162 (= R164), T201 (= T197), P202 (= P198), M205 (= M201), A212 (≠ P208), V227 (= V223), Y229 (≠ T225)
binding (1S,3R,4S,5R)-1,3,4,5-tetrahydroxycyclohexanecarboxylic acid: N66 (≠ V68), T68 (= T70), K72 (= K74), N93 (= N95), D109 (= D111)

Sites not aligning to the query:

Query Sequence

>WP_012046899.1 NCBI__GCF_000015165.1:WP_012046899.1
MIPVPTGATRLHIIIGDPIAQVRAPAGVSRAFAERGSDAILVPVQVAPVDLAGFLATATR
VKNLDGIVATIPHKFACYRACATATERAHFIGAVNLMRRSPNGGWHGDMVDGLGFVGAAR
AKGFDPNGTRALQIGAGGAGSAIALALIDAGVRELAIHDSDAGRRDALIARLNERGRGRA
LIGAMDATGFDLVANATPAGMQPGDPLPVDMATVSPSAYCGCVITRPEVSPFIAAARERG
CLTATGTDMYRALERVMVDFLLSRQPPS

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory