SitesBLAST
Comparing WP_012049033.1 NCBI__GCF_000016765.1:WP_012049033.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
2hk9B Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
35% identity, 94% coverage: 14:286/289 of query aligns to 11:267/267 of 2hk9B
- binding nadp nicotinamide-adenine-dinucleotide phosphate: V67 (≠ I71), G130 (= G135), G133 (= G138), A134 (= A139), N153 (≠ A158), R154 (= R159), T155 (≠ D160), K158 (= K163), T188 (= T206), S189 (= S207), V190 (≠ L208), I214 (= I232), M238 (= M258), L239 (= L259)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S22), S21 (= S24), N64 (= N68), T66 (= T70), K70 (= K74), N91 (= N95), D106 (= D110), Y216 (= Y234), L239 (= L259), Q242 (= Q262)
2hk9A Crystal structure of shikimate dehydrogenase from aquifex aeolicus in complex with shikimate and NADP+ at 2.2 angstrom resolution (see paper)
35% identity, 94% coverage: 14:286/289 of query aligns to 11:267/269 of 2hk9A
- binding 2'-monophosphoadenosine-5'-diphosphate: V67 (≠ I71), G132 (= G137), G133 (= G138), A134 (= A139), N153 (≠ A158), R154 (= R159), T155 (≠ D160), T188 (= T206), S189 (= S207), V190 (≠ L208)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S19 (= S22), S21 (= S24), N64 (= N68), K70 (= K74), N91 (= N95), D106 (= D110), Y216 (= Y234), L239 (= L259), Q242 (= Q262)
O67049 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Aquifex aeolicus (strain VF5) (see paper)
35% identity, 94% coverage: 14:286/289 of query aligns to 11:267/269 of O67049
- SLS 19:21 (≠ SKS 22:24) binding shikimate
- D82 (≠ P86) binding NADP(+)
- N91 (= N95) binding shikimate
- D106 (= D110) binding shikimate
- GAGGA 130:134 (≠ GTGGA 135:139) binding NADP(+)
- I214 (= I232) binding NADP(+)
- Y216 (= Y234) binding shikimate
- G235 (= G255) binding NADP(+)
- Q242 (= Q262) binding shikimate
Q9KVT3 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)
35% identity, 95% coverage: 14:287/289 of query aligns to 10:274/278 of Q9KVT3
- SKS 18:20 (= SKS 22:24) binding shikimate
- N90 (= N95) binding shikimate
- D106 (= D110) binding shikimate
- NRTFAK 154:159 (≠ ARDLDK 158:163) binding NADP(+)
- Q248 (= Q262) binding shikimate
3pgjA 2.49 angstrom resolution crystal structure of shikimate 5- dehydrogenase (aroe) from vibrio cholerae o1 biovar eltor str. N16961 in complex with shikimate
35% identity, 95% coverage: 14:287/289 of query aligns to 6:270/272 of 3pgjA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S22), S16 (= S24), N59 (= N68), T61 (= T70), K65 (= K74), N86 (= N95), D102 (= D110), Q244 (= Q262)
Q58484 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) (Methanococcus jannaschii) (see paper)
38% identity, 90% coverage: 14:272/289 of query aligns to 11:264/282 of Q58484
1nvtB Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
38% identity, 90% coverage: 14:272/289 of query aligns to 16:269/287 of 1nvtB
- active site: K75 (= K74), D111 (= D110)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I72 (= I71), G135 (= G135), G137 (= G137), G138 (= G138), A139 (= A139), N157 (≠ A158), R158 (= R159), T159 (≠ D160), K162 (= K163), A200 (≠ T205), T201 (= T206), P202 (≠ S207), I203 (≠ L208), M205 (= M210), L229 (≠ I232), Y231 (= Y234), M255 (= M258), L256 (= L259)
- binding zinc ion: E22 (≠ G20), H23 (= H21)
1nvtA Crystal structure of shikimate dehydrogenase (aroe or mj1084) in complex with NADP+ (see paper)
38% identity, 90% coverage: 14:272/289 of query aligns to 16:269/287 of 1nvtA
- active site: K75 (= K74), D111 (= D110)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G135 (= G135), A139 (= A139), N157 (≠ A158), R158 (= R159), T159 (≠ D160), K162 (= K163), A200 (≠ T205), T201 (= T206), P202 (≠ S207), I203 (≠ L208), M205 (= M210), L229 (≠ I232), Y231 (= Y234), G252 (= G255), M255 (= M258), L256 (= L259)
2cy0A Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP (see paper)
37% identity, 94% coverage: 14:284/289 of query aligns to 6:258/262 of 2cy0A
- active site: K64 (= K74), D100 (= D110)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G123 (= G135), G126 (= G138), A127 (= A139), N146 (≠ A158), R147 (= R159), T148 (≠ D160), R151 (≠ K163), T179 (= T206), R180 (≠ S207), V181 (≠ L208), L205 (≠ I232), L232 (= L259)
2ev9B Crystal structure of shikimate 5-dehydrogenase (aroe) from thermus thermophilus hb8 in complex with NADP(h) and shikimate (see paper)
37% identity, 94% coverage: 14:284/289 of query aligns to 6:258/263 of 2ev9B
- active site: K64 (= K74), D100 (= D110)
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: S14 (= S22), S16 (= S24), N58 (= N68), T60 (= T70), K64 (= K74), N85 (= N95), D100 (= D110), Q235 (= Q262)
Q5SJF8 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8) (see paper)
37% identity, 94% coverage: 14:284/289 of query aligns to 6:258/263 of Q5SJF8
- SLS 14:16 (≠ SKS 22:24) binding shikimate
- T60 (= T70) binding shikimate
- K64 (= K74) active site, Proton acceptor
- N85 (= N95) binding shikimate
- D100 (= D110) binding shikimate
- GAGGA 123:127 (≠ GTGGA 135:139) binding NADP(+)
- NRTPQR 146:151 (≠ ARDLDK 158:163) binding NADP(+)
- L205 (≠ I232) binding NADP(+)
- Y207 (= Y234) binding shikimate
- G228 (= G255) binding NADP(+)
- Q235 (= Q262) binding shikimate
Q5HNV1 Shikimate dehydrogenase (NADP(+)); SDH; EC 1.1.1.25 from Staphylococcus epidermidis (strain ATCC 35984 / RP62A) (see paper)
31% identity, 88% coverage: 14:268/289 of query aligns to 5:245/269 of Q5HNV1
- SLS 13:15 (≠ SKS 22:24) binding shikimate
- T60 (= T70) binding shikimate
- N85 (= N95) binding shikimate
- D100 (= D110) binding shikimate
- Y211 (= Y234) Plays a major role in the catalytic process and a minor role in the substrate binding; mutation to F: Leads to a 345-fold decrease in the catalytic efficiency and a 3-fold decrease in the affinity binding for shikimate.
- Q239 (= Q262) binding shikimate
Q9SQT8 Bifunctional 3-dehydroquinate dehydratase/shikimate dehydrogenase, chloroplastic; DHQ-SDH protein; DHQase-SORase; Protein EMBRYO DEFECTIVE 3004; EC 4.2.1.10; EC 1.1.1.25 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
32% identity, 92% coverage: 14:278/289 of query aligns to 328:594/603 of Q9SQT8
- S336 (= S22) binding shikimate; mutation to A: 13-fold decrease in substrate affinity but almost no change in activity.
- S338 (= S24) binding shikimate; mutation to A: 10-fold decrease in activity, and 9-fold decrease in substrate affinity.
- T381 (= T70) binding shikimate
- K385 (= K74) binding shikimate; mutation to A: Strongly reduced shikimate dehydrogenase activity, but minor change in substrate affinity.; mutation to N: Strongly reduced shikimate dehydrogenase activity, but no change in substrate affinity.
- N406 (= N95) binding shikimate
- D423 (= D110) binding shikimate; mutation to A: Loss of shikimate dehydrogenase activity.; mutation to N: Reduced shikimate dehydrogenase activity, but no change in substrate affinity.
- A461 (vs. gap) binding NADP(+)
- G463 (vs. gap) binding NADP(+)
- A464 (vs. gap) binding NADP(+)
- N483 (≠ A158) binding NADP(+)
- T485 (≠ D160) binding NADP(+)
- R488 (≠ K163) binding NADP(+)
- M525 (= M210) binding NADP(+)
- A548 (≠ I232) binding NADP(+)
- Y550 (= Y234) binding shikimate; mutation Y->F,A: 100-fold decrease in activity, and 2-fold decrease in substrate affinity.
- G571 (= G255) binding NADP(+)
- Q578 (= Q262) binding shikimate
- Q582 (≠ A266) binding shikimate
Sites not aligning to the query:
- 124 binding 3-dehydroshikimate
- 126 binding 3-dehydroshikimate
- 155 binding 3-dehydroshikimate
- 241 binding 3-dehydroshikimate
- 279 binding 3-dehydroshikimate
- 300 binding 3-dehydroshikimate
- 304 binding 3-dehydroshikimate
2o7qA Crystal structure of the a. Thaliana dhq-dehydroshikimate-sdh- shikimate-NADP(h)
31% identity, 92% coverage: 14:278/289 of query aligns to 239:483/501 of 2o7qA
Sites not aligning to the query:
2o7sA Crystal structure of the a. Thaliana dhq-dehydroshikimate-sdh- shikimate-NADP(h)
35% identity, 92% coverage: 14:278/289 of query aligns to 239:482/500 of 2o7sA
- binding 3-dehydroshikimate: I239 (≠ V14), S247 (= S22), S249 (= S24), T292 (= T70), K296 (= K74), N317 (= N95), D334 (= D110), Y438 (= Y234), Q466 (= Q262), Q470 (≠ A266)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: I293 (= I71), P294 (= P72), K296 (= K74), D334 (= D110), G354 (= G137), G355 (= G138), A356 (= A139), N374 (≠ A158), R375 (= R159), T376 (≠ D160), R379 (≠ K163), T409 (= T206), S410 (= S207), M411 (≠ L208), A436 (≠ I232), M462 (= M258), F463 (≠ L259)
Sites not aligning to the query:
2gptA Crystal structure of arabidopsis dehydroquinate dehydratase-shikimate dehydrogenase in complex with tartrate and shikimate (see paper)
35% identity, 92% coverage: 14:278/289 of query aligns to 239:480/498 of 2gptA
- binding (3r,4s,5r)-3,4,5-trihydroxycyclohex-1-ene-1-carboxylic acid: I239 (≠ V14), S247 (= S22), S249 (= S24), T292 (= T70), K296 (= K74), N317 (= N95), D334 (= D110), Y436 (= Y234), Q464 (= Q262), Q468 (≠ A266)
Sites not aligning to the query:
P15770 Shikimate dehydrogenase (NADP(+)); SD; SDH; EC 1.1.1.25 from Escherichia coli (strain K12) (see paper)
33% identity, 90% coverage: 14:272/289 of query aligns to 6:254/272 of P15770
1nytA Shikimate dehydrogenase aroe complexed with NADP+ (see paper)
33% identity, 90% coverage: 14:272/289 of query aligns to 6:254/271 of 1nytA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: K65 (= K74), D102 (= D110), G128 (= G137), G129 (= G138), A130 (= A139), N149 (≠ A158), R150 (= R159), T151 (≠ D160), R154 (≠ K163), T188 (= T206), S189 (= S207), S190 (≠ L208), M213 (≠ I232), G237 (= G255), M240 (= M258), L241 (= L259)
1o9bA Quinate/shikimate dehydrogenase ydib complexed with nadh (see paper)
32% identity, 90% coverage: 18:276/289 of query aligns to 10:270/280 of 1o9bA
- binding 1,4-dihydronicotinamide adenine dinucleotide: A126 (≠ T136), G127 (= G137), G128 (= G138), A129 (= A139), R150 (≠ A158), F154 (vs. gap), K199 (≠ S207), V200 (≠ L208), M202 (= M210), C226 (≠ I232), Y228 (= Y234), M252 (= M258), L253 (= L259)
1npdB X-ray structure of shikimate dehydrogenase complexed with NAD+ from e.Coli (ydib) northeast structural genomics research consortium (nesg) target er24 (see paper)
32% identity, 90% coverage: 18:276/289 of query aligns to 16:276/288 of 1npdB
- binding nicotinamide-adenine-dinucleotide: A132 (≠ T136), G133 (= G137), G134 (= G138), A135 (= A139), N155 (≠ A157), R156 (≠ A158), D158 (= D160), F160 (vs. gap), T204 (= T206), K205 (≠ S207), V206 (≠ L208), M208 (= M210), C232 (≠ I232), M258 (= M258), L259 (= L259)
Query Sequence
>WP_012049033.1 NCBI__GCF_000016765.1:WP_012049033.1
MSELPFAPGAFAEVIGDPIGHSKSPTIHKFWLDRAGIPADYRAAHVKPEDLWIYFEERRR
DPAWRGCNVTIPHKQAVMKLVDRIDPAAERIGAVNTVMPEDGKLVGYNTDYAGFLEPIRP
LLAQQHLFRMARVFGTGGAAKAVVLALHDHGFTIVVAARDLDKARALREAFEPDERLIAP
IEIFADPTDFPFDDREGILDLVVNTTSLGMNGQPPLALDFSHVPPDAVIYDIVYAPLETP
LLAEAKRHGLRTIDGLHMLIGQAAAAFERFYGRPAPRDADDALRALLVA
Or try a new SitesBLAST search
SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory