SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012049426.1 NCBI__GCF_000016765.1:WP_012049426.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 9 hits to proteins with known functional sites (download)

6n2oC 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
28% identity, 92% coverage: 9:576/615 of query aligns to 3:547/572 of 6n2oC

query
sites
6n2oC

N

K

D

D

F

L

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R

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A

A

N

G

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G
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S
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Y

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P

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R

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Q

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K

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Y

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L

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x
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I

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Y

A

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L

G

G

A

A

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A

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Y

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F

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G

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E

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G

A

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V

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D

S

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-

A

N

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E

E

R

L

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M

L

H

R

R

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R

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F

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D

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A

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L

-

W

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G

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A

I

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F

Y

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D

-

A

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A

A

A

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C

D

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L

K

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V

F

I

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N

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D

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A

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Q

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P

binding succinyl-coenzyme a: G14 (= G20), G16 (= G22), I17 (≠ S23), I18 (≠ A24), S19 (= S25), F41 (= F47), A43 (≠ S49), E44 (≠ N50), I45 (= I51), R128 (≠ I138), N131 (= N141), F157 (≠ Y167), N168 (= N177), T226 (= T235), R302 (= R324)
binding thiamine diphosphate: P224 (= P233), I225 (= I234), E252 (= E274)

6n2oA 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
28% identity, 92% coverage: 9:576/615 of query aligns to 3:547/572 of 6n2oA

query
sites
6n2oA

N

K

D

D

F

L

V

I

V

I

K

R

F

V

A

A

N

G

V

E

N

G

G
|
G

S

E

G
|
G

S

I

A

I

S
|
S

A

S

N

G

G

D

L

F

F

I

V

A

K

A

A

A

I

C

L

A

R

R

M

A

G

G

V

L

P

E

V

V

G

Y

A

T

R

F

N

K

I

T

F

F

P

P

S

A

N

E

I

I

Q

K

G

G

L

G

P

Y

T

A

W

M

Y

Y

E

Q

I

V

R

R

V

A

S

S

G

S

H

E

G

K

W

L

L

Y

G

C

R

Q

R

G

G

D

G

T

V

F

D

D

L

V

M

F

V

C

A

A

M

F

N

N

P

G

Q

E

T

A

W

Y

D

E

R

Q

D

N

V

K

A

D

E

K

I

I

E

K

P

P

G

G

G

T

Y

A

L

F

F

V

Y

Y

D

D

S

-

T

-

K

-

P

-

M

Y

P

P

S

G

S

G

K

D

F

F

R

E

D

P

D

D

I

E

T

I

V

P

V

E

G

G

L

V

P

F

L

A

T

Y

A

P

L

I

C

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M

A

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A

Q

Y

T

A

A

E

K

P

E

S

M

K

K

R

S

K

Y

I

R

L

S

K

K

N
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N

I

M

I

V

Y

A

L

L

G

G

A

A

L

L

T

S

F

E

L

L

L

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N

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A

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x
K

Y

F

A

G

A

K

K

K

P

G

A

E

L

E

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P

L

A

A

-

F

N

N

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E

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A

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A

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Y

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P

I
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T

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A

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A

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-

C

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H

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K

T

C

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G

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A

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Y

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E

I

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A

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R

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A

A

A

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A

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K

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-

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E

V

L

D

T

G

E

D

D

G

N

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P

R

M

T

A

L

A

P

P

A

G

T

T

H

P

P

N

S

A

K

K

G

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F

I

T

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G

G

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E

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G

A

A

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D

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P

-

A

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R

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H

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A

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D

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Y

E

D

-

A

F

F

I

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A

A

A

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C

D

K

L

K

V

V

F

I

V

V

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E

E

Q

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N

N

R

F

D

Q

A

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Q

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binding 2-oxoglutaric acid: T226 (= T235), R302 (= R324)
binding coenzyme a: G14 (= G20), G16 (= G22), S19 (= S25), N131 (= N141), K156 (≠ E166)
binding thiamine diphosphate: Y223 (= Y232), P224 (= P233), I225 (= I234), E252 (= E274)

6n2nA Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
28% identity, 92% coverage: 9:576/615 of query aligns to 3:547/572 of 6n2nA

query
sites
6n2nA

N

K

D

D

F

L

V

I

V

I

K

R

F

V

A

A

N

G

V

E

N

G

G

G

S

E

G

G

S

I

A

I

S

S

A

S

N

G

G

D

L

F

F

I

V

A

K

A

A

A

I

C

L

A

R

R

M

A

G

G

V

L

P

E

V

V

G

Y

A

T

R

F

N

K

I

T

F

F

P

P

S

A

N

E

I

I

Q

K

G

G

L

G

P

Y

T

A

W

M

Y

Y

E

Q

I

V

R

R

V

A

S

S

G

S

H

E

G

K

W

L

L

Y

G

C

R

Q

R

G

G

D

G

T

V

F

D

D

L

V

M

F

V

C

A

A

M

F

N

N

P

G

Q

E

T

A

W

Y

D

E

R

Q

D

N

V

K

A

D

E

K

I

I

E

K

P

P

G

G

G

T

Y

A

L

F

F

V

Y

Y

D

D

S

-

T

-

K

-

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M

Y

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D

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D

D

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P

V

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G

L

V

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A

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N

M

A

S

A

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Y

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A

A

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P

E

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M

K

K

R

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K

Y

I

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K

K

N

N

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A

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L

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G

A

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N

N

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D

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A

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N

N

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A

A

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L

L

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A

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G

G

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A

L

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F

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A

Y
|
Y

P
|
P

I
|
I

T

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P

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A

T

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S

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L

V

A

A

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K

A

Y

F

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A

A

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-

C

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L

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H

H

D

L

P

P

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K

T

C

G

G

P

G

D

D

G

-

G

-

G

-

A

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Y

-

A

-

I

L

V

V

Q

Q

A

A

E

E

D

D

E
|
E

I

I

A

A

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S

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I

G

A

M

Q

V

V

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L

G

G

A

A

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W

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N

A

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G

A

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R

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A

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T

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A

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A

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N

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Y

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G

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I

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G

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A

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A

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G

R

L

L

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G

G

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L

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V

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K

A

A

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Y

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R

K

A

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L

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W

P

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F

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G

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A

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D

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-

A

F

F

I

G

A

A

A

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C

D

K

L

K

V

V

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I

V

V

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Q

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N

N

R

F

D

Q

A

G

Q

Q

L

L

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H

L

F

L

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N

A

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G

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P

P

binding thiamine diphosphate: Y223 (= Y232), P224 (= P233), I225 (= I234), E252 (= E274)

Q96Y66 2-oxoacid:ferredoxin oxidoreductase 1, subunit alpha; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
27% identity, 95% coverage: 19:603/615 of query aligns to 11:610/627 of Q96Y66

query
sites
Q96Y66

N

Q

G

G

S

T

G

G

S

I

A

D

S

T

A

A

N

A

G

N

L

I

F

F

V

G

K

N

A

A

I

V

L

A

R

S

M

A

G

G

V

Y

P

Y

V

I

G

Y

A

G

R

N

N

R

I

E

F

Y

P

Y

S
|
S

N

N

I

I

Q

K

G

G

L

R

P

H

T

S

W

Y

Y

F

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V

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G

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N

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N

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|
T

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L

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A

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N

N

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K

G

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M

F

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x
D

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D

N

P

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A

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L

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R

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G

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A

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-

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L

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N

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F

A

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A

L

L

L

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V

I

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A

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F

F

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P

P

F

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-

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K

G

N

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A

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A

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H

R

D

D

L

K

V

I

F

I

V

T

V

V

E

E

Q

G

N

N

R

Y

D

L

A

A

Q

Q

L

T

R

S

T

L

L

L

L

V

I

K

N

M

E

Y

G

T

G

G

V

K

D

D

P

V

A

T

R

N

L

-

V

-

K

S

V

I

L

L

N

K

Y

W

D

N

G

G

S

R

P

P

I

-

T

-

A

-

R

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F

F

I

L

Q

R

A

D

E

E

L

L

A

E

K

E

G

A

M

L

S41 (= S49) mutation to A: Same oxidoreductase activity as the wild-type.
T349 (= T329) mutation to L: Same oxidoreductase activity as the wild-type.
D468 (≠ T456) mutation to A: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.

P72578 2-oxoacid:ferredoxin oxidoreductase subunit alpha; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see 2 papers)
27% identity, 95% coverage: 19:603/615 of query aligns to 11:610/632 of P72578

query
sites
P72578

N

Q

G

G

S

T

G

G

S

I

A

D

S

T

A

A

N

A

G

N

L

I

F

F

V

G

K

N

A

A

I

V

L

A

R

S

M

A

G

G

V

Y

P

Y

V

I

G

Y

A

G

R

N

N

R

I

E

F

Y

P

Y

S

S

N

N

I

I

Q

K

G

G

L

G

P

H

T

S

W

Y

Y

F

E

S

I

L

R

T

V

I

S

S

G

D

H

K

G

R

W

V

L

R

G

S

R

N

R

T

G

Q

G

K

V

I

D

D

L

I

M

L

V

V

A

S

M

F

N

D

P

A

Q

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T

T

W

V

D

F

R

Q

D

H

V

F

A

Y

E

D

I

V

E

K

P

D

G

-

G

-

Y

I

L

L

F

I

Y

Y

D

N

-

K

-

A

-

V

-

E

-

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-

T

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K

-

I

-

D

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A

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Q

S

S

T

M

K

E

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P

M

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L

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A

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F

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K

D

D

D

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L

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T

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K

V

Q

G

G

L

Y

P

E

L

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T

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-

V

-

K

-

G

-

A

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L

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Y

-

A

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S

-

K

-

N

-

N

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V

A

T

L

L

C

I

N

P

A

V

A

N

Y

Y

A

D

E

E

P

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A

K

K

R

K

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A

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M

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P

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L

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-

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E

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L

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K

K

N

N

I

I

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G

L

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A

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K

L

L

L

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G

G

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R

N

A

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A

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A

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K

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N

N

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N

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Y

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|
Y

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|
P

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|
I

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|
T

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|
P

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|
T

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A

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A

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F

A

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D

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A

L

L

L

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V

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A

M

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P

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G

N

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A

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N

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L

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G

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P

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F

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Y253 (= Y232) mutation Y->A,W: Loss of oxidoreductase activity.; mutation to F: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
YPITP 253:257 (= YPITP 232:236) YPITP motif
P254 (= P233) mutation to G: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
I255 (= I234) mutation I->L,M,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
T256 (= T235) mutation T->A,S,V: Significant decrease in the catalytic efficiency, while the affinity for 2-oxoacid is only slightly affected.
P257 (= P236) mutation P->A,G,V: Loss of oxidoreductase activity.
R344 (= R324) mutation R->A,K: Loss of oxidoreductase activity.
T353 (= T333) Plays a key role in the broad substrate specificity; mutation T->I,V: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.

5b48A 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
26% identity, 95% coverage: 19:603/615 of query aligns to 11:562/576 of 5b48A

query
sites
5b48A

N

Q

G

G

S

T

G

G

S

I

A

D

S

T

A

A

N

A

G

N

L

I

F

F

V

G

K

N

A

A

I

V

L

A

R

S

M

A

G

G

V

Y

P

Y

V

I

G

Y

A

G

R

N

N

R

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F

Y

P

Y

S

S

N

N

I

I

Q

K

G

G

L

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F

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Y

Y
|
Y

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|
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|
I

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|
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|
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D

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I

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V

V

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G

N

N

R

Y

D

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A

A

Q

Q

L

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S

T

L

L

L

L

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I

K

N

M

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G

T

G

G

V

K

D

D

P

V

A

T

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N

L

-

V

-

K

S

V

I

L

L

N

K

Y

W

D

N

G

G

S

R

P

P

I

-

T

-

A

-

R

-

F

F

I

L

Q

R

A

D

E

E

L

L

A

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K

E

G

A

M

L

binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: Y221 (= Y232), P222 (= P233), I223 (= I234), E262 (= E274), R312 (= R324)

Q96XT2 2-oxoacid:ferredoxin oxidoreductase 2, subunit alpha; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
25% identity, 93% coverage: 20:591/615 of query aligns to 13:603/628 of Q96XT2

query
sites
Q96XT2

G

G

S

L

G

G

S

V

A

D

S

T

A

S

N

A

G

N

L

I

F

F

V

G

K

N

A

A

I

V

L

A

R

K

M

A

G

G

V

Y

P

Y

V

L

G

F

A

G

R

N

N

R

I

E

F

Y

P

Y

S

S

N

N

I

I

Q

K

G

G

L

R

P

H

T

S

W

Y

Y

F

E

E

I

V

R

V

V

I

S

S

G

E

H

K

G

P

W

I

L

R

G

S

R

L

R

S

G

S

G

Y

V

V

D

N

L

I

M

L

V

A

A

S

M

F

N

D

P

A

Q

E

T

T

W

V

D

F

R

Q

D

H

V

F

A

T

E

E

I

T

E

K

P

E

G

-

G

-

Y

Y

L

L

F

I

Y

Y

D

N

-

V

-

E

-

Y

-

E

-

N

-

T

-

T

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V

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D

-

L

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V

-

K

S

S

T

M

K

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P

P

M

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P

M

S

A

S

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K

Q

F

V

R

K

D

E

D

A

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L

T

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-

K

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G

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D

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Y

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R

-

R

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G

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-

K

V

V

V

I

G

G

L

F

P

N

L

Y

T

T

A

E

L

L

C

I

N

K

A

K

-

I

-

A

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D

A

T

Y

F

A

K

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P

P

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M

S

S

K

V

R

V

K

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R

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A

K

K

N

N

I

M

I

I

Y

A

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G

G

A

A

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A

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F

A

K

A

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K

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A

L

L

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I

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P

K

A

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N

N

I

T

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M

A

A

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A

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I

L

G

G

F

Y

D

N

H

S

A

V

R

-

D

-

H

-

L

-

K

-

P

P

L

N

G

V

L

Y

K

K

L

L

R

Q

R

E

A

Y

D

K

A

I

V

E

G

K

D

Q

R

R

I

I

F

Q

V

V

D

D

G

G

N

N

T

T

A

I

A

S

G

A

L

M

G

G

A

K

V

L

Y

A

G

G

G

G

A

L

T

R

V

F

C

Q

A

S

W

Y

Y

Y

P

P

I

I

T
|
T

P

P

S

A

T

S

S

D

L

E

A

S

E

V

A

Y

F

I

T

E

A

A

Y

-

C

-

R

-

K

-

L

-

R

N

H

Q

D

N

P

L

E

D

T

M

G

I

P

V

D

E

G

G

G

N

G

E

A

L

R

R

-

K

-

G

-

G

Y

V

A

V

I

V

V

V

Q

Q

A

A

E

E

D

D

E

E

I

L

A

A

S

A

I

I

G

N

M

M

V

A

T

V

G

G

A

A

G

A

W

L

N

T

G

G

A

V

R

R

A

S

F

A

T

T

C

A

T

T

S

S

G

G

P

P

G

G

V

F

S

S

L

L

M

M

Q

S

E

E

F

G

I

I

G

S

L

W

S

A

Y

G

F

M

A

N

E

E

I

V

P

P

G

V

V

V

I

I

F

T

D

Y

V

Y

Q

M

R
|
R

G

G

G

A

P

P

S

A

T

T

G

G

M

L

P

P

T

T

R

R

T

S

Q

G

Q

Q

S

A

D

D

I

L

L

K

S

F

A

A

A

L

Y

N

A

V

S

G

H

H

G

G

D

E

T

F

K

P

H

R

V

I

L

V

L

I

L

A

P

S

E

G

G

D

P

H

N

V

E

E

C

I

F

F

E

W

F

D

G

A

A

I

L

W

A

A

F

L

D

N

L

L

A

A

D

E

R

K

L

Y

Q

Q

T

T

M

P

I

V

F

I

V

H

M

I

L

I

D

E

L

K

D

T

M

L

G

A

M

-

N

N

E

A

W

Y

L

S

I

V

-

F

-

E

E

E

P

E

F

L

R

I

W

T

D

N

E

R

D

P

R

Y

V

V

L

I

D

E

R

R

G

G

K

K

I

I

M

-

T

-

A

-

E

-

M

-

L

V

E

K

A

P

G

T

A

S

D

D

F

Y

G

F

R

N

Y

R

K

F

D

E

V

V

D

T

G

E

D

D

G

G

I

I

P

S

W

-

R

-

T

-

L

-

P

P

A

R

T

V

H

F

P

L

S

G

K

Q

G

A

S

S

Y

I

F

F

T

Y

R

T

G

G

T

D

S

E

R

H

D

N

P

E

Y

E

A

G

R

H

Y

I

S

T

E

E

E

N

G

S

A

I

V

N

Y

R

V

M

D

K

N

M

M

Y

E

E

R

K

L

R

L

N

R

K

K

K

F

L

E

E

T

T

A

A

K

D

S

K

L

E

V

I

P

P

P

E

P

E

I

Q

V

R

R

V

R

N

A

I

A

V

R

G

K

D

T

A

S

D

Y

I

G

V

V

L

I

L

Y

T

Y

W

G

G

S

S

T

P

S

K

P

G

A

A

M

I

D

L

E

D

A

A

L

M

A

E

G

E

L

L

E

S

A

K

D

D

G

G

V

I

A

K

V

T

D

M

A

M

L

V

R

Q

V

V

R

K

A

M

F

F

-

N

P

P

F

Y

E

P

G

K

E

N

I

L

F

M

-

K

E

K

F

I

I

L

A

S

A

G

H

K

D

S

L

K

V

I

F

I

V

A

V

V

E

E

Q

N

N

N

R

Y

D

N

A

A

Q

Q

L

G

R

A

T

E

L

V

L

L

I

A

N

E

E

K

G

T

G

G

V

I

D

F

P

A

A

T

R

N

L

Y

V

-

K

-

V

I

L

L

N

K

Y

W

D

T

G

G

S

R

P

P

I

I

T

T

T257 (= T235) binding substrate
R344 (= R324) binding substrate

5b47A 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
25% identity, 93% coverage: 20:591/615 of query aligns to 12:602/627 of 5b47A

query
sites
5b47A

G

G

S

L

G

G

S

V

A

D

S

T

A

S

N

A

G

N

L

I

F

F

V

G

K

N

A

A

I

V

L

A

R

K

M

A

G

G

V

Y

P

Y

V

L

G

F

A

G

R

N

N

R

I

E

F

Y

P

Y

S

S

N

N

I

I

Q

K

G

G

L

R

P

H

T

S

W

Y

Y

F

E

E

I

V

R

V

V

I

S

S

G

E

H

K

G

P

W

I

L

R

G

S

R

L

R

S

G

S

G

Y

V

V

D

N

L

I

M

L

V

A

A

S

M

F

N

D

P

A

Q

E

T

T

W

V

D

F

R

Q

D

H

V

F

A

T

E

E

I

T

E

K

P

E

G

-

G

-

Y

Y

L

L

F

I

Y

Y

D

N

-

V

-

E

-

Y

-

E

-

N

-

T

-

T

-

V

-

D

-

L

-

V

-

K

S

S

T

M

K

E

P

P

M

E

P

M

S

A

S

E

K

Q

F

V

R

K

D

E

D

A

I

L

T

S

-

K

-

E

-

R

-

L

-

G

-

F

-

T

-

I

-

K

-

D

-

V

-

L

-

E

-

Y

-

L

-

K

-

R

-

R

-

G

-

V

-

K

V

V

V

I

G

G

L

F

P

N

L

Y

T

T

A

E

L

L

C

I

N

K

A

K

-

I

-

A

-

D

A

T

Y

F

A

K

E

V

P

P

-

M

S

S

K

V

R

V

K

E

I

R

L

A

K

K

N

N

I

M

I

I

Y

A

L

V

G

G

A

A

L

S

T

Y

F

G

L

L

L

L

G

G

I

L

E

K

R

F

A

D

V

Y

V

L

E

K

A

D

L

A

L

I

A

S

E

S

E

T

Y

F

A

K

A

N

K

E

P

-

A

L

L

F

I

I

P

K

A

F

N

N

I

T

E

M

A

A

L

A

Q

E

I

L

G

G

F

Y

D

N

H

S

A

V

R

-

D

-

H

-

L

-

K

-

P

P

L

N

G

V

L

Y

K

K

L

L

R

Q

R

E

A

Y

D

K

A

I

V

E

G

K

D

Q

R

R

I

I

F

Q

V

V

D

D

G

G

N

N

T

T

A

I

A

S

G

A

L

M

G

G

A

K

V

L

Y

A

G

G

G

G

A

L

T

R

V

F

C

Q

A

S

W

Y

Y
|
Y

P
|
P

I
|
I

T
|
T

P

P

S

A

T

S

S

D

L

E

A

S

E

V

A

Y

F

I

T

E

A

A

Y

-

C

-

R

-

K

-

L

-

R

N

H

Q

D

N

P

L

E

D

T

M

G

I

P

V

D

E

G

G

G

N

G

E

A

L

R

R

-

K

-

G

-

G

Y

V

A

V

I

V

V

V

Q

Q

A

A

E

E

D

D

E
|
E

I

L

A

A

S

A

I

I

G

N

M

M

V

A

T

V

G

G

A

A

G

A

W

L

N

T

G

G

A

V

R

R

A

S

F

A

T

T

C

A

T

T

S

S

G

G

P
|
P

G

G

V

F

S

S

L

L

M

M

Q

S

E

E

F

G

I

I

G

S

L

W

S

A

Y

G

F

M

A

N

E

E

I

V

P

P

G

V

V

V

I

I

F

T

D

Y

V

Y

Q

M

R
|
R

G

G

G

A

P

P

S

A

T

T

G

G

M

L

P
|
P

T

T

R

R

T

S

Q

G

Q

Q

S

A

D

D

I

L

L

K

S

F

A

A

A

L

Y

N

A

V

S

G

H

H

G

G

D

E

T

F

K

P

H

R

V

I

L

V

L

I

L

A

P

S

E

G

G

D

P

H

N

V

E

E

C

I

F

F

E

W

F

D

G

A

A

I

L

W

A

A

F

L

D

N

L

L

A

A

D

E

R

K

L

Y

Q

Q

T

T

M

P

I

V

F

I

V

H

M

I

L

I

D

E

L

K

D

T

M

L

G

A

M

-

N

N

E

A

W

Y

L

S

I

V

-

F

-

E

E

E

P

E

F

L

R

I

W

T

D

N

E

R

D

P

R

Y

V

V

L

I

D

E

R

R

G

G

K

K

I

I

M

-

T

-

A

-

E

-

M

-

L

V

E

K

A

P

G

T

A

S

D

D

F

Y

G

F

R

N

Y

R

K

F

D

E

V

V

D

T

G

E

D

D

G

G

I

I

P

S

W

-

R

-

T

-

L

-

P

P

A

R

T

V

H

F

P

L

S

G

K

Q

G

A

S

S

Y

I

F

F

T

Y

R

T

G

G

T

D

S

E

R

H

D

N

P

E

Y

E

A

G

R

H

Y

I

S

T

E

E

E

N

G

S

A

I

V

N

Y

R

V

M

D

K

N

M

M

Y

E

E

R

K

L

R

L

N

R

K

K

K

F

L

E

E

T

T

A

A

K

D

S

K

L

E

V

I

P

P

P

E

P

E

I

Q

V

R

R

V

R

N

A

I

A

V

R

G

K

D

T

A

S

D

Y

I

G

V

V

L

I

L

Y

T

Y

W

G

G

S

S

T

P

S

K

P

G

A

A

M

I

D

L

E

D

A

A

L

M

A

E

G

E

L

L

E

S

A

K

D

D

G

G

V

I

A

K

V

T

D

M

A

M

L

V

R

Q

V

V

R

K

A

M

F

F

-

N

P

P

F

Y

E

P

G

K

E

N

I

L

F

M

-

K

E

K

F

I

I

L

A

S

A

G

H

K

D

S

L

K

V

I

F

I

V

A

V

V

E

E

Q

N

N

N

R

Y

D

N

A

A

Q

Q

L

G

R

A

T

E

L

V

L

L

I

A

N

E

E

K

G

T

G

G

V

I

D

F

P

A

A

T

R

N

L

Y

V

-

K

-

V

I

L

L

N

K

Y

W

D

T

G

G

S

R

P

P

I

I

T

T

binding pyruvic acid: T256 (= T235), R343 (= R324), P351 (= P332)
binding thiamine diphosphate: Y253 (= Y232), P254 (= P233), I255 (= I234), E293 (= E274), P317 (= P298)

5b46A 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
25% identity, 93% coverage: 20:591/615 of query aligns to 12:602/627 of 5b46A

query
sites
5b46A

G

G

S

L

G

G

S

V

A

D

S

T

A

S

N

A

G

N

L

I

F

F

V

G

K

N

A

A

I

V

L

A

R

K

M

A

G

G

V

Y

P

Y

V

L

G

F

A

G

R

N

N

R

I

E

F

Y

P

Y

S

S

N

N

I

I

Q

K

G

G

L

R

P

H

T

S

W

Y

Y

F

E

E

I

V

R

V

V

I

S

S

G

E

H

K

G

P

W

I

L

R

G

S

R

L

R

S

G

S

G

Y

V

V

D

N

L

I

M

L

V

A

A

S

M

F

N

D

P

A

Q

E

T

T

W

V

D

F

R

Q

D

H

V

F

A

T

E

E

I

T

E

K

P

E

G

-

G

-

Y

Y

L

L

F

I

Y

Y

D

N

-

V

-

E

-

Y

-

E

-

N

-

T

-

T

-

V

-

D

-

L

-

V

-

K

S

S

T

M

K

E

P

P

M

E

P

M

S

A

S

E

K

Q

F

V

R

K

D

E

D

A

I

L

T

S

-

K

-

E

-

R

-

L

-

G

-

F

-

T

-

I

-

K

-

D

-

V

-

L

-

E

-

Y

-

L

-

K

-

R

-

R

-

G

-

V

-

K

V

V

V

I

G

G

L

F

P

N

L

Y

T

T

A

E

L

L

C

I

N

K

A

K

-

I

-

A

-

D

A

T

Y

F

A

K

E

V

P

P

-

M

S

S

K

V

R

V

K

E

I

R

L

A

K

K

N

N

I

M

I

I

Y

A

L

V

G

G

A

A

L

S

T

Y

F

G

L

L

L

L

G

G

I

L

E

K

R

F

A

D

V

Y

V

L

E

K

A

D

L

A

L

I

A

S

E

S

E

T

Y

F

A

K

A

N

K

E

P

-

A

L

L

F

I

I

P

K

A

F

N

N

I

T

E

M

A

A

L

A

Q

E

I

L

G

G

F

Y

D

N

H

S

A

V

R

-

D

-

H

-

L

-

K

-

P

P

L

N

G

V

L

Y

K

K

L

L

R

Q

R

E

A

Y

D

K

A

I

V

E

G

K

D

Q

R

R

I

I

F

Q

V

V

D

D

G

G

N

N

T

T

A

I

A

S

G

A

L

M

G

G

A

K

V

L

Y

A

G

G

G

G

A

L

T

R

V

F

C

Q

A

S

W

Y

Y
|
Y

P
|
P

I
|
I

T

T

P

P

S

A

T

S

S

D

L

E

A

S

E

V

A

Y

F

I

T

E

A

A

Y

-

C

-

R

-

K

-

L

-

R

N

H

Q

D

N

P

L

E

D

T

M

G

I

P

V

D

E

G

G

G

N

G

E

A

L

R

R

-

K

-

G

-

G

Y

V

A

V

I

V

V

V

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E

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A

V

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N

N

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D

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A

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A

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A

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N

L

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-

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G

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P

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T

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binding thiamine diphosphate: Y253 (= Y232), P254 (= P233), I255 (= I234), E293 (= E274)

Query Sequence

>WP_012049426.1 NCBI__GCF_000016765.1:WP_012049426.1
MTRPITAVNDFVVKFANVNGSGSASANGLFVKAILRMGVPVGARNIFPSNIQGLPTWYEI
RVSGHGWLGRRGGVDLMVAMNPQTWDRDVAEIEPGGYLFYDSTKPMPSSKFRDDITVVGL
PLTALCNAAYAEPSKRKILKNIIYLGALTFLLGIERAVVEALLAEEYAAKPALIPANIEA
LQIGFDHARDHLKPLGLKLRRADAVGDRIFVDGNTAAGLGAVYGGATVCAWYPITPSTSL
AEAFTAYCRKLRHDPETGPDGGGARYAIVQAEDEIASIGMVTGAGWNGARAFTCTSGPGV
SLMQEFIGLSYFAEIPGVIFDVQRGGPSTGMPTRTQQSDILSAAYASHGDTKHVLLLPEG
PNECFEFGALAFDLADRLQTMIFVMLDLDMGMNEWLIEPFRWDEDRVLDRGKIMTAEMLE
AGADFGRYKDVDGDGIPWRTLPATHPSKGSYFTRGTSRDPYARYSEEGAVYVDNMERLLR
KFETAKSLVPPPIVRRAARKTSYGVIYYGSTSPAMDEALAGLEADGVAVDALRVRAFPFE
GEIFEFIAAHDLVFVVEQNRDAQLRTLLINEGGVDPARLVKVLNYDGSPITARFIQAELA
KGMGVPKARAGEMVR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory