SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012049427.1 NCBI__GCF_000016765.1:WP_012049427.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 17 hits to proteins with known functional sites (download)

Q96XT4 2-oxoacid:ferredoxin oxidoreductase 2, subunit beta; OFOR2; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see paper)
37% identity, 65% coverage: 34:261/351 of query aligns to 12:248/304 of Q96XT4

query
sites
Q96XT4

C
|
C

A

P

G

G

C
|
C

G

G

H

D

D

F

S

G

I

I

S

L

A

R

A

A

I

E

V

E

Q

M

A

A

C

I

F

R

E

E

I

L

D

G

L

I

E

N

P

P

H

K

R

S

L

V

A

V

K

I

L

V

S

S

G

G

I
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I

G
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G

C
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C

S
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S

S

G

K

K

T

I

P

P

D

H

Y

F

F

M

L

N

G

L

A

P

S

I

H

S

G

G

F

V

N

H

T

T

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L

H

H

G

G

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R

M

S

P

I

S

A

V

F

L

A

T

T

G

G

A

I

N

K

L

L

A

S

N

N

R

P

S

S

L

L

I

E

Y

V

L

I

G

V

V

N

S

V

G

G

D
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D

G
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G

D
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D

S

G

A

L

S

G

I

I

G

G

L

M

G

G

Q

H

F

F

A

V

H

H

A

L

M

G

R

R

G

N

V

I

N

D

M

I

T

A

Y

V

I

L

V

V

E

H

N

N

N
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N

G

G

V
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V

Y

Y

G
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G

L
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L

T

T

K

K

G

G

Q

Q

F

A

S

S

A

P

T

T

A

L

D

H

K

R

G

G

S

E

K

K

S

T

K

K

K

S

-

L

G

P

V

K

V

P

N

N

S

I

D

M

S

D

G

A

I

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D

N

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V

L

G

A

L

V

A

A

L

L

Q

A

L

A

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G

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Y

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T

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V

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A

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R

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Y

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Y

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E

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L

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H

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K

G

G

A

S

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A

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D

V

I

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L

S

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P

P

C
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C

I

P

A

T

F

Y

N

N

N

D

H

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K

I

G

N

S

T

T

K

K

E

S

W

F

Y

D

D

-

K

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R

-

V

-

Y

-

K

-

L

-

D

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N

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V

-

P

-

G

W

W

-

D

-

P

-

V

V

V

R

R

E

K

H

E

N

E

E

E

A

A

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Q

N

K

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K

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F

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-

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M

-

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M

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E

C12 (= C34) binding [4Fe-4S] cluster
C15 (= C37) binding [4Fe-4S] cluster
IGCS 44:47 (= IGCS 66:69) binding thiamine diphosphate
C46 (= C68) binding [4Fe-4S] cluster
D90 (= D112) binding Mg(2+)
GD 91:92 (= GD 113:114) binding thiamine diphosphate
N118 (= N140) binding Mg(2+)
V120 (= V142) binding Mg(2+)
GL 122:123 (= GL 144:145) binding thiamine diphosphate
C197 (= C218) binding [4Fe-4S] cluster

5b46B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - ligand free form (see paper)
37% identity, 65% coverage: 34:261/351 of query aligns to 9:245/301 of 5b46B

query
sites
5b46B

C
|
C

A

P

G

G

C
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C

G

G

H

D

D

F

S

G

I

I

S

L

A

R

A

A

I

E

V

E

Q

M

A

A

C

I

F

R

E

E

I

L

D

G

L

I

E

N

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P

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K

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S

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V

A

V

K

I

L

V

S

S

G

G

I
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I

G
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G

C
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C

S
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S

S

G

K

K

T

I

P

P

D

H

Y

F

F

M

L

N

G

L

A

P

S

I

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S

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G

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H
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H

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G

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R

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G

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G

D
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D

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D
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S

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I

G

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G

Q

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H

A

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R

G

N

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N

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M

I

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A

Y

V

I

L

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V

E

H

N

N

N
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N

G

G

V
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V

Y
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Y

G
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G

L
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L

T
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T

K

K

G

G

Q

Q

F

A

S

S

A

P

T

T

A

L

D

H

K

R

G

G

S

E

K

K

S

T

K

K

K

S

-

L

G

P

V

K

V

P

N

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M

S

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G

A

I

V

D

N

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P

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A

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A

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A

L

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F

F

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Y

D

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K

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E

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L

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I

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K

A

A

L

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T

L

H

H

K

K

G

G

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A

A

F

L

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V

D

D

V

I

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L

S

Q

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P

C
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C

I

P

A
x
T

F
x
Y

N

N

N

D

H

-

K

I

G

N

S

T

T

K

K

E

S

W

F

Y

D

D

-

K

-

R

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V

-

Y

-

K

-

L

-

D

-

N

-

V

-

P

-

G

W

W

-

D

-

P

-

V

V

V

R

R

E

K

H

E

N

E

E

E

A

A

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Q

N

K

R

K

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D

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V

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L

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M

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A

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A

A

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K

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S

Y

Y

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E

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W

G

G

S

E

binding magnesium ion: D87 (= D112), N115 (= N140), V117 (= V142)
binding iron/sulfur cluster: C9 (= C34), C12 (= C37), C43 (= C68), C194 (= C218), T196 (≠ A220), Y197 (≠ F221)
binding thiamine diphosphate: I41 (= I66), G42 (= G67), C43 (= C68), S44 (= S69), H62 (= H87), G86 (= G111), G88 (= G113), D89 (= D114), N115 (= N140), V117 (= V142), Y118 (= Y143), G119 (= G144), L120 (= L145), T121 (= T146)

Sites not aligning to the query:

binding iron/sulfur cluster: 8

6n2oD 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
36% identity, 64% coverage: 16:241/351 of query aligns to 6:227/291 of 6n2oD

query
sites
6n2oD

H

H

N

K

A

M

L

E

G

N

F

M

T

K

R

P

R

K

D

D

Y

Y

E

K

G

S

K

E

V

V

-

P

S

T

T

T

L
x
W

C
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C

A

P

G

G

C
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C

G

G

H

H

D

F

S

G

I

I

S

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A

N

A

G

I

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V

Y

Q

R

A

A

C

M

F

A

E

E

I

L

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G

L

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D

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H

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K

L

F

A

A

K

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S

S

G

G

I
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I

G
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G

C
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C

S
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S

S

S

K
x
R

T

M

P

P

D

-

Y

Y

F

F

L

V

G

D

A

-

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S

H

Y

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K

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M

N

H

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T

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H
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H

G

G

R

R

M

A

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G

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A

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V

L

A

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T

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G

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R

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C

Y

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L

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G

V

V

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G

G

D
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D

G
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G

D
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S

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G

G

G

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M

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P

H

H

A

M

M

A

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R

K

G

N

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V

N

N

M

M

T

T

Y

Y

I

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V

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E

M

N

D

N
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N

G

G

V
x
I

Y

Y

G
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G

L
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L

T
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T

K
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K

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G

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Q

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Y

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A

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T

A

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Y

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G

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L

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I

K

K

A

G

A

A

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T

E

H

H

K

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G

A

F

A

A

F

Y

I

V

D

N

V

I

L

F

S

S

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Q

C
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C

I

P

A
x
T

F
|
F

N

N

N

K

H

-

K

-

G

-

S

-

T

I

K

D

S

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D

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I

binding magnesium ion: D101 (= D112), N129 (= N140), I131 (≠ V142)
binding succinyl-coenzyme a: I57 (= I66), R62 (≠ K71), L134 (= L145), K136 (= K147)
binding iron/sulfur cluster: W24 (≠ L33), C25 (= C34), C28 (= C37), C59 (= C68), C208 (= C218), T210 (≠ A220), F211 (= F221)
binding thiamine diphosphate: I57 (= I66), G58 (= G67), C59 (= C68), S60 (= S69), H76 (= H87), G102 (= G113), D103 (= D114), N129 (= N140), I131 (≠ V142), G133 (= G144), L134 (= L145), T135 (= T146)

6n2oB 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus with 2-oxoglutarate, coenzyme a and succinyl-coa bound (see paper)
36% identity, 64% coverage: 16:241/351 of query aligns to 6:227/291 of 6n2oB

query
sites
6n2oB

H

H

N

K

A

M

L

E

G

N

F

M

T

K

R

P

R

K

D

D

Y

Y

E

K

G

S

K

E

V

V

-

P

S

T

T

T

L
x
W

C
|
C

A

P

G

G

C
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C

G

G

H

H

D

F

S

G

I

I

S

L

A

N

A

G

I

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V

Y

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R

A

A

C

M

F

A

E

E

I

L

D

G

L

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E

D

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H

T

R

K

L

F

A

A

K

A

L

I

S

S

G

G

I
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I

G
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G

C
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C

S
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S

S

S

K
x
R

T

M

P

P

D

-

Y

Y

F

F

L

V

G

D

A

-

S

S

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Y

G

K

F

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N

H

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H
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H

G

G

R

R

M

A

P

G

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A

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V

L

A

T

T

G

G

A

T

N

Q

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A

A

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R

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L

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C

Y

V

L

V

G

V

V

A

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G

G

D
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D

G
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G

D
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D

S

G

A

F

S

S

I

I

G

G

L

G

G

G

Q

H

F

M

A

P

H

H

A

M

M

A

R

R

R

K

G

N

V

V

N

N

M

M

T

T

Y

Y

I

V

V

L

E

M

N

D

N
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N

G

G

V
x
I

Y
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Y

G
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G

L
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L

T
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T

K
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K

G

G

Q

Q

F

Y

S

S

A

P

T

T

A

S

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D

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K

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G

M

S

T

K

A

S
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Y

K

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V

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S

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F

F

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G

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K

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E

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K

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A

F

A

A

F

Y

I

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N

V

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Q

C
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C

I

P

A
x
T

F
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F

N

N

N

K

H

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K

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G

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I

binding 2-oxoglutaric acid: R62 (≠ K71), L134 (= L145)
binding coenzyme a: K136 (= K147), Y150 (≠ S160)
binding magnesium ion: D101 (= D112), N129 (= N140), I131 (≠ V142)
binding iron/sulfur cluster: W24 (≠ L33), C25 (= C34), C28 (= C37), C59 (= C68), C208 (= C218), T210 (≠ A220), F211 (= F221)
binding thiamine diphosphate: I57 (= I66), G58 (= G67), C59 (= C68), S60 (= S69), H76 (= H87), G102 (= G113), D103 (= D114), N129 (= N140), I131 (≠ V142), Y132 (= Y143), G133 (= G144), L134 (= L145), T135 (= T146)

6n2nB Crystal structure of 2-oxoglutarate:ferredoxin oxidoreductase from magnetococcus marinus (see paper)
36% identity, 64% coverage: 16:241/351 of query aligns to 6:227/291 of 6n2nB

query
sites
6n2nB

H

H

N

K

A

M

L

E

G

N

F

M

T

K

R

P

R

K

D

D

Y

Y

E

K

G

S

K

E

V

V

-

P

S

T

T

T

L
x
W

C
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C

A

P

G

G

C
|
C

G

G

H
|
H

D

F

S

G

I

I

S

L

A

N

A

G

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V

Y

Q

R

A

A

C

M

F

A

E

E

I

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D

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L

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E

D

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H

T

R

K

L

F

A

A

K

A

L

I

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S

G

G

I
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I

G
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G

C
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C

S
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S

S

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K

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T

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P

P

D

-

Y

Y

F

F

L

V

G

D

A

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S

S

H

Y

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K

F

M

N

H

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T

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L

H
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H

G

G

R

R

M

A

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G

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A

V

V

L

A

T

T

G

G

A

T

N

Q

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A

A

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R

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L

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Y

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L

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V

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G

D
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D

G
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G

D
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D

S

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F

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S

I

I

G

G

L

G

G

G

Q

H

F

M

A

P

H

H

A

M

M

A

R

R

R

K

G

N

V

V

N

N

M

M

T

T

Y

Y

I

V

V

L

E

M

N

D

N
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N

G

G

V
x
I

Y
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Y

G
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G

L
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L

T
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T

K

K

G

G

Q

Q

F

Y

S

S

A

P

T

T

A

S

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R

D

P

K

E

G

M

S

T

K

A

S

Y

K

T

G

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Y

V

G

N

G

S

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D

E

S

N

G

P

I

M

D

N

M

P

V

L

G

L

L

Y

A

M

L

L

Q

T

L

Y

G

G

A

A

T

T

F

Y

V

V

A

A

R

Q

S

A

F

F

S

A

G

G

D

K

K

P

D

K

Q

D

L

C

V

A

P

E

L

L

I

I

K

K

A

G

A

A

L

M

T

E

H

H

K

E

G

G

A

F

A

A

F

Y

I

V

D

N

V

I

L

F

S

S

P

Q

C
|
C

I

P

A
x
T

F
|
F

N

N

N

K

H

-

K

-

G

-

S

-

T

I

K

D

S

T

F

V

D

D

W

F

V

Y

R

R

E

D

H

L

N

V

E

E

A

P

V

I

binding magnesium ion: D101 (= D112), N129 (= N140), I131 (≠ V142)
binding iron/sulfur cluster: W24 (≠ L33), C25 (= C34), C28 (= C37), H30 (= H39), C59 (= C68), C208 (= C218), T210 (≠ A220), F211 (= F221)
binding thiamine diphosphate: I57 (= I66), G58 (= G67), C59 (= C68), S60 (= S69), H76 (= H87), G100 (= G111), D101 (= D112), G102 (= G113), D103 (= D114), N129 (= N140), I131 (≠ V142), Y132 (= Y143), G133 (= G144), L134 (= L145), T135 (= T146)

Q96Y68 2-oxoacid:ferredoxin oxidoreductase 1, subunit beta; OFOR1; EC 1.2.7.11 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 2 papers)
39% identity, 63% coverage: 26:247/351 of query aligns to 4:219/305 of Q96Y68

query
sites
Q96Y68

Y

F

E

K

G

P

K

Q

V

W

S

N

T

D

L

W

C
|
C

A

P

G

G

C
|
C

G

G

H

N

D

F

S

G

I

I

S

L

A

N

A

A

I

E

V

Q

Q

Q

A

A

C

I

F

V

E

E

I

L

D

G

L

V

E

D

P

T

H

K

R

N

L

V

A

V

K

V

L

V

S

S

G

G

I

I

G

G

C
|
C

S

S

S

G

K
|
K

T

I

P

P

D

H

Y

F

F

F

L

R

G

T

A

P

S

I

H

S

G

G

F

V

N

H

T

T

V

L

H

H

G

G

R

R

M

A

P

I

S

A

V

F

L

A

T

T

G

G

A

I

N

K

L

L

A

S

N

N

R

P

S

D

L

L

I

V

Y

V

L

I

G

V

V

N

S

G

G

G

D
|
D

G
|
G

D
|
D

S

L

A

L

S

G

I

I

G

G

L

A

G

G

Q

H

F

F

A

V

H

A

A

A

M

G

R

R

G

N

V

V

N

D

M

M

T

V

Y

V

I

I

V

L

E

H

N

D

N
|
N

G

G

V
|
V

Y

Y

G
|
G

L
|
L

T

T

K
|
K

G

G

Q

Q

F

A

S

S

A

P

T

T

A

L

D

K

K

R

G

G

S

E

K

K

S

P

K

K

K

S

-

L

G

P

V

R

V

P

N

N

S

I

D

N

S

D

G

A

I

V

D

N

M

P

V

I

G

A

L

L

A

A

L

I

Q

S

L

S

G

G

A

Y

T

T

F

F

V

V

A

A

R

R

S

G

F

Y

S

A

G

Y

D

D

K

V

D
x
K

Q

H

L

L

V

K

P

E

L

L

I

I

K

K

A

S

A

A

L

I

T

K

H

H

K

K

G

G

A

L

A

A

F

L

I

I

D

D

V

V

L

L

S

Q

P

P

C
|
C

I

P

A

T

F

Y

N

N

N

D

H

-

K

-

G

-

S

-

T

I

K

N

S

T

F

K

D

E

W

W

V

-

R

-

E

-

H

Y

N

D

E

K

A

R

V

I

N

Y

R

K

L

L

D

D

V

T

L

L

C12 (= C34) binding [4Fe-4S] cluster
C15 (= C37) binding [4Fe-4S] cluster
C46 (= C68) binding [4Fe-4S] cluster
K49 (= K71) mutation to I: Loss of oxidoreductase activity toward 2-oxoglutarate but retains its activity toward pyruvate.
D90 (= D112) binding Mg(2+)
GD 91:92 (= GD 113:114) binding thiamine diphosphate
N118 (= N140) binding Mg(2+)
V120 (= V142) binding Mg(2+)
GL 122:123 (= GL 144:145) binding thiamine diphosphate
K125 (= K147) Plays an important role in the binding of CoA; mutation to A: Shows a strong decrease of affinity for CoA and a poor inactivation by 4-fluoro-7-nitrobenzofurazan (NBD-F).
K173 (≠ D194) mutation to A: Same oxidoreductase activity as the wild-type.
C197 (= C218) binding [4Fe-4S] cluster

P72579 2-oxoacid:ferredoxin oxidoreductase subunit beta; OFOR; EC 1.2.7.11 from Sulfolobus sp. (see paper)
40% identity, 61% coverage: 33:247/351 of query aligns to 11:219/305 of P72579

query
sites
P72579

L

W

C

C

A

P

G

G

C

C

G

G

H

N

D

F

S

G

I

I

S

L

A

N

A

A

I

E

V

Q

Q

Q

A

A

C

I

F

V

E

E

I

L

D

G

L

V

E

D

P

T

H

K

R

N

L

V

A

V

K

V

L

V

S

S

G

G

I

I

G

G

C

C

S

S

S

G

K
|
K

T

I

P

P

D

H

Y

F

F

F

L

R

G

T

A

P

S

I

H

S

G

G

F

V

N

H

T

T

V

L

H

H

G

G

R

R

M

A

P

I

S

A

V

F

L

A

T

T

G

G

A

I

N

K

L

L

A

S

N

N

R

P

S

D

L

L

I

V

Y

V

L

I

G

V

V

N

S

G

G

G

D

D

G

G

D

D

S

L

A

L

S

G

I

I

G

G

L

A

G

G

Q

H

F

F

A

V

H

A

A

A

M

G

R

R

G

N

V

V

N

D

M

M

T

V

Y

V

I

I

V

L

E

H

N

D

N

N

G

G

V

V

Y

Y

G

G

L
|
L

T

T

K

K

G

G

Q

Q

F

A

S

S

A

P

T

T

A

L

D

K

K

R

G

G

S

E

K

K

S

P

K

K

K

S

-

L

G

P

V

R

V

P

N

N

S

I

D

N

S

D

G

A

I

V

D

N

M

P

V

I

G

A

L

L

A

A

L

I

Q

S

L

S

G

G

A

Y

T

T

F

F

V

V

A

A

R

R

S

G

F

Y

S

A

G

Y

D

D

K

V

D

K

Q

H

L

L

V

K

P

E

L

L

I

I

K

K

A

S

A

A

L

I

T

K

H

H

K

K

G

G

A

L

A

A

F

L

I

I

D

D

V

V

L

L

S

Q

P

P

C

C

I

P

A

T

F

Y

N

N

N

D

H

-

K

-

G

-

S

-

T

I

K

N

S

T

F

K

D

E

W

W

V

-

R

-

E

-

H

Y

N

D

E

K

A

R

V

I

N

Y

R

K

L

L

D

D

V

T

L

L

K49 (= K71) mutation to I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to R: Increase the oxidoreductase activity with pyruvate.; mutation to V: Slight decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.
L123 (= L145) mutation L->A,I: Strong decrease of the oxidoreductase activity with pyruvate, 2-oxobutyrate and 2-oxoglutarate.; mutation to N: Strong decrease of the oxidoreductase activity with pyruvate and 2-oxobutyrate. However, this mutant shows almost the same activity with 2-oxoglutarate as the wild-type.

5b47B 2-oxoacid:ferredoxin oxidoreductase 2 from sulfolobus tokodai - pyruvate complex (see paper)
37% identity, 65% coverage: 34:261/351 of query aligns to 5:219/275 of 5b47B

query
sites
5b47B

C
|
C

A

P

G

G

C
|
C

G

G

H

D

D

F

S

G

I

I

S

L

A

R

A

A

I

E

V

E

Q

M

A

A

C

I

F

R

E

E

I

L

D

G

L

I

E

N

P

P

H

K

R

S

L

V

A

V

K

I

L

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

K

K

T

I

P

P

D

H

Y

F

F

M

L

N

G

L

A

P

S

I

H

S

G

G

F

V

N

H

T

T

V

L

H
|
H

G

G

R

R

M

S

P

I

S

A

V

F

L

A

T

T

G

G

A

I

N

K

L

L

A

S

N

N

R

P

S

S

L

L

I

E

Y

V

L

I

G

V

V

N

S

V

G

G

D
|
D

G
|
G

D
|
D

S

G

A

L

S

G

I

I

G

G

L

M

G

G

Q

H

F

F

A

V

H

H

A

L

M

G

R

R

G

N

V

I

N

D

M

I

T

A

Y

V

I

L

V

V

E

H

N

N

N
|
N

G

G

V
|
V

Y
|
Y

G

G

L
|
L

T

-

K

-

G

-

Q

Q

F

A

S

S

A

P

T

T

A

L

D

H

K

R

G

G

S

E

K

-

S

-

K

-

G

-

V

-

N

N

S

I

D

M

S

D

G

A

I

V

D

N

M

P

V

L

G

A

L

V

A

A

L

L

Q

A

L

A

G

G

A

Y

T

T

F

F

V

V

A

A

R

R

S

G

F

Y

S

A

G

Y

D

D

K

V

D

M

Q

H

L

L

V

K

P

E

L

L

I

I

K

K

A

K

A

A

L

I

T

L

H

H

K

K

G

G

A

S

A

A

F

L

I

V

D

D

V

I

L

L

S

Q

P

P

C
|
C

I

P

A

T

F

Y

N

R

N

V

H

Y

K

K

-

L

G

D

S

N

T

V

K

P

S

G

F

W

D

D

-

P

W

V

V

V

R

R

E

K

H

E

N

E

E

E

A

A

V

Q

N

K

R

K

L

F

D

E

V

-

L

-

M

-

A

-

R

-

M

Q

A

A

V

I

T

M

A

K

S

S

Y

Y

E

E

P

W

G

G

S

E

binding magnesium ion: D83 (= D112), N111 (= N140)
binding iron/sulfur cluster: C5 (= C34), C8 (= C37), C39 (= C68), C179 (= C218)
binding thiamine diphosphate: I37 (= I66), G38 (= G67), C39 (= C68), S40 (= S69), H58 (= H87), G84 (= G113), D85 (= D114), N111 (= N140), V113 (= V142), Y114 (= Y143), L116 (= L145)

5b48B 2-oxoacid:ferredoxin oxidoreductase 1 from sulfolobus tokodai (see paper)
43% identity, 54% coverage: 33:223/351 of query aligns to 7:188/286 of 5b48B

query
sites
5b48B

L

W

C
|
C

A

P

G

G

C
|
C

G

G

H

N

D

F

S

G

I

I

S

L

A

N

A

A

I

E

V

Q

Q

Q

A

A

C

I

F

V

E

E

I

L

D

G

L

V

E

D

P

T

H

K

R

N

L

V

A

V

K

V

L

V

S

S

G

G

I
|
I

G
|
G

C
|
C

S
|
S

S

G

K

K

T

I

P

P

D

-

Y

H

F

F

L

T

G

P

A

I

S

S

H

-

G

G

F

V

N

H

T

T

V

L

H
|
H

G

G

R

R

M

A

P

I

S

A

V

F

L

A

T

T

G

G

A

I

N

K

L

L

A

S

N

N

R

P

S

D

L

L

I

V

Y

V

L

I

G

V

V

N

S

G

G

G

D
|
D

G
|
G

D
|
D

S

L

A

L

S

G

I

I

G

G

L

A

G

G

Q

H

F

F

A

V

H

A

A

A

M

G

R

R

G

N

V

V

N

D

M

M

T

V

Y

V

I

I

V

L

E

H

N

D

N
|
N

G

G

V
|
V

Y
|
Y

G
|
G

L
|
L

T

T

K

K

G

G

Q

Q

F

A

S

S

A

P

T

T

A

L

D

K

K

R

G

G

S

E

K

-

S

-

K

-

G

-

V

-

N

N

S

I

D

N

S

D

G

A

I

V

D

N

M

P

V

I

G

A

L

L

A

A

L

I

Q

S

L

S

G

G

A

Y

T

T

F

F

V

V

A

A

R

R

S

G

F

Y

S

A

G

Y

D

D

K

V

D

K

Q

H

L

L

V

K

P

E

L

L

I

I

K

K

A

S

A

A

L

I

T

K

H

H

K

K

G

G

A

L

A

A

F

L

I

I

D

D

V

V

L

L

S

Q

P

P

C
|
C

I

P

A
x
T

F

Y

N

N

N

D

binding magnesium ion: D84 (= D112), N112 (= N140), V114 (= V142)
binding iron/sulfur cluster: C8 (= C34), C11 (= C37), C42 (= C68), C183 (= C218), T185 (≠ A220)
binding 2-[(2E)-3-[(4-azanyl-2-methyl-pyrimidin-5-yl)methyl]-4-methyl-2-(1-oxidanylpropylidene)-1,3-thiazol-5-yl]ethyl phosphono hydrogen phosphate: I40 (= I66), G41 (= G67), C42 (= C68), S43 (= S69), H59 (= H87), G85 (= G113), D86 (= D114), N112 (= N140), V114 (= V142), Y115 (= Y143), G116 (= G144), L117 (= L145)

7plmA Cryoem reconstruction of pyruvate ferredoxin oxidoreductase (pfor) in anaerobic conditions (see paper)
25% identity, 41% coverage: 88:232/351 of query aligns to 907:1052/1177 of 7plmA

query
sites
7plmA

G

G

R

L

M

L

P

G

S

Q

V

I

L

A

T

A

G

M

A

S

N

D

L

L

A

Y

N

T

R

K

S

K

L

S

I

V

Y

W

L

I

G

-

V

F

S

G

G
|
G

D
|
D

G
|
G

D

W

S

A

A

Y

S

D

I

I

G

G

L

Y

G

G

Q

G

F

L

A

D

H

H

A

V

M

L

R

A

R

S

G

G

V

E

N
x
D

M

V

T
x
N

Y

V

I

F

V

V

E

M

N

D

N
x
T

G

E

V
|
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

F

S

S

S

A

K

T

A

A

T

D

P

K

T

G

G

S

A

K

V

S

A

K

K

K

F

G

A

V

A

N

G

S

K

D

R

S

T

G

G

-

K

I

K

D

D

M

L

V

A

G

R

L

M

A

V

L

M

Q

T

L

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

R

T

-

V

S

S

F

M

S

G

G

Y

D

S

K

K

D

Q

Q

Q

L

F

V

L

P

K

L

V

I

L

K

K

A

E

A

A

L

E

T

S

H
x
F

K

P

G
|
G

A

P

A

S

F

L

I

V

D

I

V

A

L

Y

S

A

P

T

C
|
C

I
|
I

A

N

F

Q

N

G

N

L

H

R

K

K

G

G

S

M

T

G

K

K

S

S

F

Q

D

D

binding calcium ion: D950 (≠ N132), N952 (≠ T134), F1026 (≠ H206), G1028 (= G208)
binding magnesium ion: D930 (= D112), T958 (≠ N140), V960 (= V142), S962 (≠ G144)
binding iron/sulfur cluster: S962 (≠ G144), C1038 (= C218), I1039 (= I219)
binding thiamine diphosphate: G929 (= G111), D930 (= D112), G931 (= G113), V960 (= V142), Y961 (= Y143), S962 (≠ G144), N963 (≠ L145), T964 (= T146)

Sites not aligning to the query:

binding iron/sulfur cluster: 458, 657, 659, 664, 665, 666, 667, 668, 670, 674, 675, 679, 708, 715, 716, 717, 718, 719, 721, 725, 726, 731, 732, 781, 782, 785, 787, 810
binding thiamine diphosphate: 27, 28, 29, 63, 787, 808, 809, 810, 839

2c3uA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus, oxygen inhibited form (see paper)
25% identity, 41% coverage: 88:232/351 of query aligns to 939:1084/1231 of 2c3uA

query
sites
2c3uA

G

G

R

L

M

L

P

G

S

Q

V

I

L

A

T

A

G

M

A

S

N

D

L

L

A

Y

N

T

R

K

S

K

L

S

I

V

Y

W

L

I

G

-

V

F

S

G

G
|
G

D
|
D

G
|
G

D
x
W

S

A

A

Y

S

D

I

I

G

G

L

Y

G

G

Q

G

F

L

A

D

H

H

A

V

M

L

R

A

R

S

G

G

V

E

N
x
D

M

V

T
x
N

Y

V

I

F

V

V

E

M

N

D

N
x
T

G

E

V
|
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

F

S

S

S

A

K

T

A

A

T

D

P

K

T

G

G

S

A

K

V

S

A

K

K

K

F

G

A

V

A

N

G

S

K

D

R

S

T

G

G

-

K

I

K

D

D

M

L

V

A

G

R

L

M

A

V

L

M

Q

T

L

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

R

T

-

V

S

S

F

M

S

G

G

Y

D

S

K

K

D

Q

Q

Q

L

F

V

L

P

K

L

V

I

L

K

K

A

E

A
|
A

L
x
E

T

S

H
x
F

K

P

G
|
G

A

P

A
x
S

F

L

I

V

D

I

V

A

L

Y

S

A

P

T

C
|
C

I
|
I

A

N

F

Q

N

G

N

L

H

R

K

K

G

G

S

M

T

G

K

K

S

S

F

Q

D

D

active site: N995 (≠ L145)
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: G961 (= G111), D962 (= D112), G963 (= G113), W964 (≠ D114), V992 (= V142), Y993 (= Y143), S994 (≠ G144), N995 (≠ L145), T996 (= T146)
binding calcium ion: D982 (≠ N132), N984 (≠ T134), A1055 (= A203), E1056 (≠ L204), F1058 (≠ H206), G1060 (= G208), S1062 (≠ A210)
binding magnesium ion: D962 (= D112), T990 (≠ N140), V992 (= V142)
binding pyruvic acid: N995 (≠ L145)
binding iron/sulfur cluster: C1070 (= C218), I1071 (= I219)

Sites not aligning to the query:

active site: 30, 63, 113
binding 2-(3-{[4-(hydroxyamino)-2-methylpyrimidin-5-yl]methyl}-4-methyl-2,3-dihydro-1,3-thiazol-5-yl)ethyl trihydrogen diphosphate: 28, 29, 63, 87, 816, 837, 839, 868, 869
binding pyruvic acid: 30, 113, 122
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 811, 814, 816, 839

2c3pA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
25% identity, 41% coverage: 88:232/351 of query aligns to 939:1084/1231 of 2c3pA

query
sites
2c3pA

G

G

R

L

M

L

P

G

S

Q

V

I

L

A

T

A

G

M

A

S

N

D

L

L

A

Y

N

T

R

K

S

K

L

S

I

V

Y

W

L

I

G

-

V

F

S

G

G
|
G

D
|
D

G
|
G

D

W

S

A

A

Y

S

D

I

I

G

G

L

Y

G

G

Q

G

F

L

A

D

H

H

A

V

M

L

R

A

R

S

G

G

V

E

N
x
D

M
x
V

T
x
N

Y

V

I

F

V

V

E

M

N

D

N
x
T

G

E

V
|
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

F

S

S

S

A

K

T

A

A

T

D

P

K

T

G

G

S

A

K

V

S

A

K

K

K

F

G

A

V

A

N

G

S

K

D

R

S

T

G

G

-

K

I

K

D

D

M

L

V

A

G

R

L

M

A

V

L

M

Q

T

L

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

R

T

-

V

S

S

F

M

S

G

G

Y

D

S

K

K

D

Q

Q

Q

L

F

V

L

P

K

L

V

I

L

K

K

A

E

A
|
A

L
x
E

T

S

H
x
F

K

P

G
|
G

A

P

A
x
S

F

L

I

V

D

I

V

A

L

Y

S

A

P

T

C
|
C

I
|
I

A

N

F

Q

N

G

N

L

H

R

K

K

G

G

S

M

T

G

K

K

S

S

F

Q

D

D

active site: N995 (≠ L145)
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: G961 (= G111), D962 (= D112), G963 (= G113), V992 (= V142), Y993 (= Y143), S994 (≠ G144), N995 (≠ L145), T996 (= T146)
binding calcium ion: D982 (≠ N132), V983 (≠ M133), N984 (≠ T134), A1055 (= A203), E1056 (≠ L204), F1058 (≠ H206), G1060 (= G208), S1062 (≠ A210)
binding magnesium ion: D962 (= D112), T990 (≠ N140), V992 (= V142)
binding iron/sulfur cluster: S994 (≠ G144), C1070 (= C218), I1071 (= I219)

Sites not aligning to the query:

active site: 30, 63, 113
binding 1-(2-{(2s,4r,5r)-3-[(4-amino-2-methylpyrimidin-5-yl)methyl]-2-[(1s)-1-carboxy-1-hydroxyethyl]-4-methyl-1,3-thiazolidin-5-yl}ethoxy)-1,1,3,3-tetrahydroxy-1lambda~5~-diphosphox-1-en-2-ium 3-oxide: 28, 29, 30, 63, 87, 113, 816, 837, 838, 839, 868, 869
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839

2c3oA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
25% identity, 41% coverage: 88:232/351 of query aligns to 939:1084/1231 of 2c3oA

query
sites
2c3oA

G

G

R

L

M

L

P

G

S

Q

V

I

L

A

T

A

G

M

A

S

N

D

L

L

A

Y

N

T

R

K

S

K

L

S

I

V

Y

W

L

I

G

-

V

F

S

G

G
|
G

D
|
D

G
|
G

D

W

S

A

A

Y

S

D

I

I

G

G

L

Y

G

G

Q

G

F

L

A

D

H

H

A

V

M

L

R

A

R

S

G

G

V

E

N
x
D

M

V

T
x
N

Y

V

I

F

V

V

E

M

N

D

N
x
T

G

E

V
|
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

F

S

S

S

A

K

T

A

A

T

D

P

K

T

G

G

S

A

K

V

S

A

K

K

K

F

G

A

V

A

N

G

S

K

D

R

S

T

G

G

-

K

I

K

D

D

M

L

V

A

G

R

L

M

A

V

L

M

Q

T

L

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

R

T

-

V

S

S

F

M

S

G

G

Y

D

S

K

K

D

Q

Q

Q

L

F

V

L

P

K

L

V

I

L

K

K

A

E

A

A

L
x
E

T

S

H
x
F

K

P

G
|
G

A

P

A

S

F

L

I

V

D

I

V

A

L

Y

S

A

P

T

C
|
C

I
|
I

A

N

F

Q

N

G

N

L

H

R

K

K

G

G

S

M

T

G

K

K

S

S

F

Q

D

D

active site: N995 (≠ L145)
binding calcium ion: D982 (≠ N132), N984 (≠ T134), E1056 (≠ L204), F1058 (≠ H206), G1060 (= G208)
binding magnesium ion: D962 (= D112), T990 (≠ N140), V992 (= V142)
binding pyruvic acid: T996 (= T146)
binding iron/sulfur cluster: C1070 (= C218), I1071 (= I219)
binding thiamine diphosphate: G961 (= G111), D962 (= D112), G963 (= G113), V992 (= V142), Y993 (= Y143), S994 (≠ G144), N995 (≠ L145), T996 (= T146)

Sites not aligning to the query:

active site: 30, 63, 113
binding pyruvic acid: 30, 113
binding iron/sulfur cluster: 458, 681, 688, 689, 691, 692, 694, 698, 699, 702, 703, 744, 746, 747, 748, 749, 750, 754, 760, 761, 810, 811, 814, 816, 839
binding thiamine diphosphate: 28, 29, 63, 87, 816, 837, 838, 839, 868

1kekA Crystal structure of the free radical intermediate of pyruvate:ferredoxin oxidoreductase (see paper)
25% identity, 41% coverage: 88:232/351 of query aligns to 939:1084/1231 of 1kekA

query
sites
1kekA

G

G

R

L

M

L

P

G

S

Q

V

I

L

A

T

A

G

M

A

S

N

D

L

L

A

Y

N

T

R

K

S

K

L

S

I

V

Y

W

L

I

G

-

V

F

S

G

G
|
G

D
|
D

G
|
G

D

W

S

A

A

Y

S

D

I

I

G

G

L

Y

G

G

Q

G

F

L

A

D

H

H

A

V

M

L

R

A

R

S

G

G

V

E

N
x
D

M

V

T
x
N

Y

V

I

F

V

V

E

M

N

D

N
x
T

G

E

V
|
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

F

S

S

S

A

K

T

A

A

T

D

P

K

T

G

G

S

A

K

V

S

A

K

K

K

F

G

A

V

A

N

G

S

K

D

R

S

T

G

G

-

K

I

K

D

D

M

L

V

A

G

R

L

M

A

V

L

M

Q

T

L

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

R

T

-

V

S

S

F

M

S

G

G

Y

D

S

K

K

D

Q

Q

Q

L

F

V

L

P

K

L

V

I

L

K

K

A

E

A
|
A

L
x
E

T

S

H
x
F

K

P

G

G

A

P

A
x
S

F

L

I

V

D

I

V

A

L

Y

S

A

P

T

C
|
C

I
|
I

A

N

F

Q

N

G

N

L

H

R

K

K

G

G

S

M

T

G

K

K

S

S

F

Q

D

D

active site: N995 (≠ L145)
binding calcium ion: D982 (≠ N132), N984 (≠ T134), A1055 (= A203), E1056 (≠ L204), F1058 (≠ H206), S1062 (≠ A210)
binding 2-acetyl-thiamine diphosphate: G961 (= G111), D962 (= D112), G963 (= G113), V992 (= V142), Y993 (= Y143), S994 (≠ G144), N995 (≠ L145), T996 (= T146)
binding magnesium ion: D962 (= D112), T990 (≠ N140), V992 (= V142)
binding iron/sulfur cluster: C1070 (= C218), I1071 (= I219)

Sites not aligning to the query:

active site: 30, 63, 113
binding 2-acetyl-thiamine diphosphate: 29, 63, 87, 113, 816, 837, 838, 839, 868
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 703, 737, 744, 745, 746, 747, 748, 750, 754, 755, 760, 761, 810, 811, 814, 816, 839

1b0pA Crystal structure of pyruvate-ferredoxin oxidoreductase from desulfovibrio africanus (see paper)
25% identity, 41% coverage: 88:232/351 of query aligns to 939:1084/1231 of 1b0pA

query
sites
1b0pA

G

G

R

L

M

L

P

G

S

Q

V

I

L

A

T

A

G

M

A

S

N

D

L

L

A

Y

N

T

R

K

S

K

L

S

I

V

Y

W

L

I

G

-

V

F

S

G

G
|
G

D
|
D

G
|
G

D

W

S

A

A

Y

S

D

I

I

G

G

L

Y

G

G

Q

G

F

L

A

D

H

H

A

V

M

L

R

A

R

S

G

G

V

E

N
x
D

M

V

T
x
N

Y

V

I

F

V

V

E

M

N

D

N
x
T

G

E

V
|
V

Y
|
Y

G
x
S

L
x
N

T
|
T

K

G

G

G

Q

Q

F

S

S

S

A

K

T

A

A

T

D

P

K

T

G

G

S

A

K

V

S

A

K

K

K

F

G

A

V

A

N

G

S

K

D

R

S

T

G

G

-

K

I

K

D

D

M

L

V

A

G

R

L

M

A

V

L

M

Q

T

L

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

R

T

-

V

S

S

F

M

S

G

G

Y

D

S

K

K

D

Q

Q

Q

L

F

V

L

P

K

L

V

I

L

K

K

A

E

A
|
A

L
x
E

T

S

H
x
F

K

P

G
|
G

A

P

A

S

F

L

I

V

D

I

V

A

L

Y

S

A

P

T

C
|
C

I
|
I

A

N

F

Q

N

G

N

L

H

R

K

K

G

G

S

M

T

G

K

K

S

S

F

Q

D

D

active site: N995 (≠ L145)
binding calcium ion: D982 (≠ N132), N984 (≠ T134), A1055 (= A203), E1056 (≠ L204), F1058 (≠ H206), G1060 (= G208)
binding magnesium ion: D962 (= D112), T990 (≠ N140), V992 (= V142)
binding iron/sulfur cluster: S994 (≠ G144), C1070 (= C218), I1071 (= I219)
binding thiamine diphosphate: G961 (= G111), D962 (= D112), G963 (= G113), V992 (= V142), Y993 (= Y143), S994 (≠ G144), N995 (≠ L145), T996 (= T146)

Sites not aligning to the query:

active site: 30, 63, 113
binding iron/sulfur cluster: 458, 681, 683, 688, 689, 690, 691, 692, 694, 698, 699, 702, 703, 737, 744, 745, 747, 748, 750, 754, 755, 760, 811, 814, 816, 839
binding thiamine diphosphate: 28, 29, 63, 816, 837, 838, 839, 868

P94692 Pyruvate:ferredoxin oxidoreductase; PFOR; POR; Pyruvate synthase; EC 1.2.7.1 from Desulfocurvibacter africanus (Desulfovibrio africanus) (see 3 papers)
25% identity, 41% coverage: 88:232/351 of query aligns to 940:1085/1232 of P94692

query
sites
P94692

G

G

R

L

M

L

P

G

S

Q

V

I

L

A

T

A

G

M

A

S

N

D

L

L

A

Y

N

T

R

K

S

K

L

S

I

V

Y

W

L

I

G

-

V

F

S

G

G
|
G

D
|
D

G
|
G

D
x
W

S

A

A

Y

S

D

I

I

G

G

L

Y

G

G

Q

G

F

L

A

D

H

H

A

V

M

L

R

A

R

S

G

G

V

E

N
x
D

M

V

T
x
N

Y

V

I

F

V

V

E

M

N

D

N
x
T

G
x
E

V
|
V

Y
|
Y

G
x
S

L
x
N

T

T

K

G

G

G

Q

Q

F

S

S

S

A

K

T

A

A

T

D

P

K

T

G

G

S

A

K

V

S

A

K

K

K

F

G

A

V

A

N

G

S

K

D

R

S

T

G

G

-

K

I

K

D

D

M

L

V

A

G

R

L

M

A

V

L

M

Q

T

L

Y

G

G

A

Y

T

V

F

Y

V

V

A

A

R

T

-

V

S

S

F

M

S

G

G

Y

D

S

K

K

D

Q

Q

Q

L

F

V

L

P

K

L

V

I

L

K

K

A

E

A
|
A

L

E

T

S

H
x
F

K

P

G
|
G

A

P

A
x
S

F

L

I

V

D

I

V

A

L

Y

S

A

P

T

C
|
C

I

I

A

N

F

Q

N

G

N

L

H

R

K

K

G

G

S

M

T

G

K

K

S

S

F

Q

D

D

GDGW 962:965 (≠ GDGD 111:114) binding thiamine diphosphate
D963 (= D112) binding Mg(2+)
D983 (≠ N132) binding Ca(2+)
N985 (≠ T134) binding Ca(2+)
T991 (≠ N140) binding Mg(2+)
TEVYSN 991:996 (≠ NGVYGL 140:145) binding thiamine diphosphate
V993 (= V142) binding Mg(2+)
A1056 (= A203) binding Ca(2+)
F1059 (≠ H206) binding Ca(2+)
G1061 (= G208) binding Ca(2+)
S1063 (≠ A210) binding Ca(2+)
C1071 (= C218) binding [4Fe-4S] cluster

Sites not aligning to the query:

31 binding pyruvate
64 binding thiamine diphosphate
114 binding pyruvate
689 binding [4Fe-4S] cluster
692 binding [4Fe-4S] cluster
695 binding [4Fe-4S] cluster
699 binding [4Fe-4S] cluster
745 binding [4Fe-4S] cluster
748 binding [4Fe-4S] cluster
751 binding [4Fe-4S] cluster
755 binding [4Fe-4S] cluster
812 binding [4Fe-4S] cluster
815 binding [4Fe-4S] cluster
817 binding thiamine diphosphate
840 binding [4Fe-4S] cluster; binding thiamine diphosphate

9bt4C Pyruvate:ferredoxin oxidoreductase from methanosarcina acetivorans (see paper)
23% identity, 54% coverage: 107:296/351 of query aligns to 89:264/291 of 9bt4C

query
sites
9bt4C

L

I

G

G

V

V

S

G

G
|
G

D
|
D

G
|
G

D
x
S

S

T

A

M

S

D

I

I

G

G

L

I

G

R

Q

S

F

L

A

S

H

G

A

A

M

F

R

E

R

R

G

G

V

H

N

D

M

I

T

T

Y

Y

I

V

V

C

E

V

N

D

N
|
N

G

E

V
x
A

Y
|
Y

G
x
M

L
x
N

T
|
T

K

G

G

I

Q

Q

-

R

-

S

-

G

-

T

-

P

F

F

S

D

A

A

T

S

A

T

D

T

K

T

G

S

S

P

K

A

S

G

K

K

K

V

G

S

V

F

V

G

N

N

S

P

D

R

S

P

G

K

I

K

D

D

M

M

V

P

G

A

L

I

A

M

L

A

Q

A

L

H

G

G

A

S

T

P

F

Y

V

V

A

A

R

T

S

T

F

S

S

I

G

G

D

F

K

P

D

R

Q

D

L

M

V

M

P

R

L

K

I

V

K

K

A

K

A

A

L

T

T

E

H

I

K

V

G

G

A

P

A

T

F

Y

I

I

D

H

V

S

L

H

S

A

P

P

C
|
C

I

P

A
x
T

F

G

N

W

N

G

H

F

K

D

G

G

S

S

T

K

K

T

S

-

F

-

D

-

W

-

V

-

R

-

E

-

H

-

N

-

E

-

A

-

V

-

N

-

R

-

L

-

D

-

V

I

L

E

M

I

A

A

R

K

M

L

A

A

V

V

T

E

A

T

S

C

Y

L

E

W

P

P

G

M

S

Y

L

E

T

M

E

E

V

-

T

-

Q

-

H

-

D

N

G

G

S

E

V

I

L

T

L

Q

L

V

R

R

K

K

V

V

A

K

E

D

D

S

Y

R

D

P

P

V

T

E

D

E

H

Y

A

L

G

R

A

A

I

Q

S

K

Y

R

L

F

Q

K

H

H

binding magnesium ion: D94 (= D112), N122 (= N140), A124 (≠ V142)
binding iron/sulfur cluster: M126 (≠ G144), C206 (= C218), T208 (≠ A220)
binding thiamine diphosphate: G93 (= G111), D94 (= D112), G95 (= G113), S96 (≠ D114), N122 (= N140), A124 (≠ V142), Y125 (= Y143), M126 (≠ G144), N127 (≠ L145), T128 (= T146)

Sites not aligning to the query:

binding iron/sulfur cluster: 13, 16, 17, 18, 41
binding thiamine diphosphate: 18, 39, 40, 41, 63, 64

Query Sequence

>WP_012049427.1 NCBI__GCF_000016765.1:WP_012049427.1
MTYIAKPQFHHPSLKHNALGFTRRDYEGKVSTLCAGCGHDSISAAIVQACFEIDLEPHRL
AKLSGIGCSSKTPDYFLGASHGFNTVHGRMPSVLTGANLANRSLIYLGVSGDGDSASIGL
GQFAHAMRRGVNMTYIVENNGVYGLTKGQFSATADKGSKSKKGVVNSDSGIDMVGLALQL
GATFVARSFSGDKDQLVPLIKAALTHKGAAFIDVLSPCIAFNNHKGSTKSFDWVREHNEA
VNRLDVLMARMAVTASYEPGSLTEVTQHDGSVLLLRKVAEDYDPTDHAGAISYLQHAHAA
GEIVTGLLYVDPEAEELHDALGTVKQPLNALPDEELVPPVAALDKINAGLR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory