SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012049634.1 NCBI__GCF_000016765.1:WP_012049634.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 3 hits to proteins with known functional sites (download)

2gtvX Nmr structure of monomeric chorismate mutase from methanococcus jannaschii (see paper)
33% identity, 83% coverage: 4:81/94 of query aligns to 3:88/104 of 2gtvX

query
sites
2gtvX

E

E

I

K

L

L

S

A

R

E

Y

I

R
|
R

E

K

S

K

I

I

D

D

N

E

I

I

D

D

A

N

A

K

L

I

I

L

F

K

-

A

-

R

-

W

-

P

-

W

-

A

-

E

-

K

M

L

L

I

A

A

E

E

R
|
R

F

N

K

S

I

L

T

A

Q

K

A

D

V
|
V

G

A

E

E

H

I

K
|
K

A

N

A

Q

T

L

G

G

L

I

P

P

P

I

A

N

D
|
D

P

P

G

E

R
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R

E
|
E

Q

K

I

Y

Q

I

I

Y

D

D

R

R

L

I

R

R

S

K

L

L

A

C

K

K

S

E

A

H

N

N

L

V

D

D

P

E

D

N

F

I

S

G

E

I

K

K

F

I

L

F

R

Q

F

R

V

L

I

I

D

E

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R9 (= R10), R34 (= R27), V41 (= V34), K45 (= K38), D54 (= D47), R57 (= R50), E58 (= E51)

Sites not aligning to the query:

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: 90, 94

1ecmB Atomic structure of the buried catalytic pocket of escherichia coli chorismate mutase
31% identity, 80% coverage: 10:84/94 of query aligns to 6:80/95 of 1ecmB

query
sites
1ecmB

R

R

E

E

S

K

I

I

D

S

N

A

I

L

D

D

A

E

A

K

L

L

I

L

F

A

M

L

L

L

A

A

E

E

R
|
R

F

R

K

E

I

L

T

A

Q

V

A

E

V
|
V

G

G

E

K

H

A

K
|
K

A

L

A

L

T

S

G

H

L

R

P

P

P

V

A
x
R

D
|
D

P

I

G

D

R
|
R

E
|
E

Q

R

I

D

Q

L

I

L

D

E

R

R

L

L

R

I

S

T

L

L

A

G

K

K

S

A

A

H

N

H

L

L

D

D

P

A

D

H

F

Y

S

I

E

T

K

R

F

L

L

F

R

Q

F

L

V

I

I
|
I

D

E

E

D

V
x
S

I

V

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: R23 (= R27), V30 (= V34), K34 (= K38), R42 (≠ A46), D43 (= D47), R46 (= R50), E47 (= E51), I76 (= I80), S79 (≠ V83)

Sites not aligning to the query:

binding 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid: 83

P0A9J8 Bifunctional chorismate mutase/prephenate dehydratase; Chorismate mutase-prephenate dehydratase; P-protein; EC 5.4.99.5; EC 4.2.1.51 from Escherichia coli (strain K12)
31% identity, 80% coverage: 10:84/94 of query aligns to 11:85/386 of P0A9J8

query
sites
P0A9J8

R
|
R

E

E

S

K

I

I

D

S

N

A

I

L

D

D

A

E

A

K

L

L

I

L

F

A

M

L

L

L

A

A

E

E

R
|
R

F

R

K

E

I

L

T

A

Q

V

A

E

V

V

G

G

E

K

H

A

K
|
K

A

L

A

L

T

S

G

H

L

R

P

P

P

V

A

R

D

D

P

I

G

D

R

R

E
|
E

Q

R

I

D

Q

L

I

L

D

E

R

R

L

L

R

I

S

T

L

L

A

G

K

K

S

A

A

H

N

H

L

L

D

D

P

A

D

H

F

Y

S

I

E

T

K

R

F

L

L

F

R

Q

F

L

V

I

I

I

D

E

E

D

V

S

I

V

R11 (= R10) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
R28 (= R27) mutation R->A,K: Important decrease in catalytic efficiency and affinity.
K39 (= K38) mutation K->A,Q,R: Important decrease in catalytic efficiency and affinity.
E52 (= E51) mutation E->A,D,Q: Important decrease in catalytic efficiency and affinity.

Sites not aligning to the query:

88 mutation Q->A,E,K: Important decrease in catalytic efficiency and affinity.

Query Sequence

>WP_012049634.1 NCBI__GCF_000016765.1:WP_012049634.1
MADEILSRYRESIDNIDAALIFMLAERFKITQAVGEHKAATGLPPADPGREQIQIDRLRS
LAKSANLDPDFSEKFLRFVIDEVIHHHRRAGADG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory