SitesBLAST
Comparing WP_012050210.1 NCBI__GCF_000016765.1:WP_012050210.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
4jnqA Crystal structure of a thioredoxin reductase from brucella melitensis
59% identity, 96% coverage: 1:311/323 of query aligns to 2:312/315 of 4jnqA
- active site: C137 (= C137), C140 (= C140), D141 (= D141)
- binding dihydroflavine-adenine dinucleotide: I12 (≠ L12), G13 (= G13), S14 (= S14), G15 (= G15), P16 (= P16), A17 (= A17), A36 (≠ Q36), G37 (= G37), Q39 (= Q39), G42 (= G42), Q43 (= Q43), L44 (= L44), N52 (= N52), I85 (= I85), A113 (= A113), T114 (= T114), C140 (= C140), G283 (= G282), D284 (= D283), R291 (= R290), Q292 (= Q291), A293 (= A292)
5vt3B High resolution structure of thioredoxin-disulfide reductase from vibrio vulnificus cmcp6 in complex with NADP and fad
54% identity, 96% coverage: 1:310/323 of query aligns to 3:315/319 of 5vt3B
- active site: C138 (= C137), C141 (= C140), D142 (= D141)
- binding flavin-adenine dinucleotide: G15 (= G13), S16 (= S14), G17 (= G15), P18 (= P16), A19 (= A17), T38 (≠ Q36), G39 (= G37), Q41 (= Q39), G44 (= G42), Q45 (= Q43), L46 (= L44), T49 (= T47), N54 (= N52), H86 (≠ T84), I87 (= I85), S114 (≠ A113), T115 (= T114), G116 (= G115), E162 (= E161), H247 (= H245), G287 (= G282), D288 (= D283), R295 (= R290), Q296 (= Q291), A297 (= A292), S300 (≠ A295)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L122 (= L121), G156 (= G155), G157 (= G156), N158 (= N157), T159 (= T158), H178 (= H177), R179 (= R178), R180 (= R179), R184 (= R183), I245 (= I243), G246 (= G244), R295 (= R290), Q296 (= Q291)
P0A9P4 Thioredoxin reductase; TRXR; EC 1.8.1.9 from Escherichia coli (strain K12) (see 2 papers)
55% identity, 96% coverage: 2:310/323 of query aligns to 3:314/321 of P0A9P4
- 36:43 (vs. 36:43, 50% identical) binding FAD
- C136 (= C137) modified: Disulfide link with 139, Redox-active
- C139 (= C140) modified: Disulfide link with 136, Redox-active
- 287:296 (vs. 283:292, 70% identical) binding FAD
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
1f6mA Crystal structure of a complex between thioredoxin reductase, thioredoxin, and the NADP+ analog, aadp+ (see paper)
55% identity, 96% coverage: 2:310/323 of query aligns to 2:313/320 of 1f6mA
- active site: S135 (≠ C137), C138 (= C140), D139 (= D141)
- binding 3-aminopyridine-adenine dinucleotide phosphate: L119 (= L121), G153 (= G155), G154 (= G156), N155 (= N157), T156 (= T158), E159 (= E161), H175 (= H177), R176 (= R178), R177 (= R179), R181 (= R183), I243 (= I243), G244 (= G244), H245 (= H245), R293 (= R290), Q294 (= Q291)
- binding flavin-adenine dinucleotide: G12 (= G13), S13 (= S14), G14 (= G15), P15 (= P16), A16 (= A17), T35 (≠ Q36), G36 (= G37), E38 (≠ Q39), G41 (= G42), Q42 (= Q43), L43 (= L44), T46 (= T47), V49 (= V50), N51 (= N52), H83 (≠ T84), I84 (= I85), A111 (= A113), T112 (= T114), G113 (= G115), H245 (= H245), G285 (= G282), D286 (= D283), R293 (= R290), Q294 (= Q291), A295 (= A292), S298 (≠ A295)
1tdfA Crystal structure of escherichia coli thioredoxin reductase refined at 2 angstrom resolution: implications for a large conformational change during catalysis (see paper)
55% identity, 96% coverage: 2:310/323 of query aligns to 2:313/316 of 1tdfA
- active site: C135 (= C137), S138 (≠ C140), D139 (= D141)
- binding flavin-adenine dinucleotide: G12 (= G13), S13 (= S14), G14 (= G15), P15 (= P16), A16 (= A17), T35 (≠ Q36), G36 (= G37), E38 (≠ Q39), G41 (= G42), Q42 (= Q43), L43 (= L44), T46 (= T47), V49 (= V50), N51 (= N52), H83 (≠ T84), I84 (= I85), A111 (= A113), T112 (= T114), S138 (≠ C140), G285 (= G282), D286 (= D283), R293 (= R290), Q294 (= Q291), A295 (= A292), S298 (≠ A295)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: L119 (= L121), I151 (= I153), T156 (= T158), E159 (= E161), H175 (= H177), R176 (= R178), R181 (= R183), E183 (= E185), I243 (= I243), G244 (= G244), H290 (≠ K287), R293 (= R290)
5u63B Crystal structure of putative thioredoxin reductase from haemophilus influenzae
55% identity, 96% coverage: 1:310/323 of query aligns to 4:316/319 of 5u63B
- active site: C139 (= C137), C142 (= C140), D143 (= D141)
- binding flavin-adenine dinucleotide: G16 (= G13), S17 (= S14), G18 (= G15), P19 (= P16), A20 (= A17), T39 (≠ Q36), G40 (= G37), Q42 (= Q39), G45 (= G42), Q46 (= Q43), L47 (= L44), T50 (= T47), N55 (= N52), H87 (≠ T84), I88 (= I85), A115 (= A113), T116 (= T114), G117 (= G115), H248 (= H245), G288 (= G282), D289 (= D283), R296 (= R290), Q297 (= Q291), A298 (= A292), S301 (≠ A295)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: R121 (≠ K119), G157 (= G155), H179 (= H177), R180 (= R178), R181 (= R179), I246 (= I243), G247 (= G244), H248 (= H245), R296 (= R290)
5uthA Crystal structure of thioredoxin reductase from mycobacterium smegmatis in complex with fad
50% identity, 94% coverage: 9:312/323 of query aligns to 6:305/306 of 5uthA
- active site: C133 (= C137), C136 (= C140), D137 (= D141)
- binding flavin-adenine dinucleotide: I9 (≠ L12), G10 (= G13), S11 (= S14), G12 (= G15), P13 (= P16), A14 (= A17), F32 (≠ V35), E33 (≠ Q36), G34 (= G37), Q36 (= Q39), G39 (= G42), A40 (≠ Q43), L41 (= L44), N49 (= N52), D81 (≠ T84), V82 (≠ I85), M110 (≠ T114), G111 (= G115), C136 (= C140), G275 (= G282), D276 (= D283), R283 (= R290), Q284 (= Q291), A285 (= A292), A288 (= A295)
8ccmA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with compound 2-06
50% identity, 94% coverage: 9:312/323 of query aligns to 5:304/305 of 8ccmA
- binding flavin-adenine dinucleotide: I8 (≠ L12), G9 (= G13), S10 (= S14), G11 (= G15), P12 (= P16), A13 (= A17), E32 (≠ Q36), G33 (= G37), Q35 (= Q39), G38 (= G42), A39 (≠ Q43), L40 (= L44), T43 (= T47), N48 (= N52), D80 (≠ T84), V81 (≠ I85), M109 (≠ T114), G110 (= G115), T131 (≠ A136), C135 (= C140), G274 (= G282), D275 (= D283), R282 (= R290), Q283 (= Q291), A284 (= A292), A287 (= A295)
- binding ~{N}6-(4-aminophenyl)-1,2-benzothiazole-3,6-diamine: R114 (≠ K119), H115 (≠ W120), L116 (= L121), R173 (= R178), E200 (≠ Q205), I201 (≠ V206), I235 (= I243)
8cclA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry a09
50% identity, 94% coverage: 9:312/323 of query aligns to 5:304/305 of 8cclA
- binding flavin-adenine dinucleotide: I8 (≠ L12), G9 (= G13), S10 (= S14), G11 (= G15), P12 (= P16), A13 (= A17), E32 (≠ Q36), G33 (= G37), Q35 (= Q39), G38 (= G42), A39 (≠ Q43), L40 (= L44), T43 (= T47), N48 (= N52), D80 (≠ T84), V81 (≠ I85), M109 (≠ T114), G110 (= G115), T131 (≠ A136), C135 (= C140), G274 (= G282), D275 (= D283), R282 (= R290), Q283 (= Q291), A284 (= A292), A287 (= A295)
- binding [1,2]thiazolo[5,4-b]pyridin-3-amine: L116 (= L121), R173 (= R178), E200 (≠ Q205), I201 (≠ V206)
8cckA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with fragment f2x-entry h07
50% identity, 94% coverage: 9:312/323 of query aligns to 5:304/305 of 8cckA
- binding flavin-adenine dinucleotide: G9 (= G13), S10 (= S14), G11 (= G15), P12 (= P16), A13 (= A17), E32 (≠ Q36), G33 (= G37), Q35 (= Q39), G38 (= G42), A39 (≠ Q43), L40 (= L44), T43 (= T47), N48 (= N52), D80 (≠ T84), V81 (≠ I85), M109 (≠ T114), G110 (= G115), T131 (≠ A136), C135 (= C140), G274 (= G282), D275 (= D283), R282 (= R290), Q283 (= Q291), A284 (= A292), A287 (= A295)
- binding ~{N}-(4-hydroxyphenyl)-2-pyrazol-1-yl-ethanamide: R114 (≠ K119), H115 (≠ W120), L116 (= L121), V148 (≠ I153), R173 (= R178), E200 (≠ Q205), I201 (≠ V206)
8ccjA Crystal structure of mycobacterium smegmatis thioredoxin reductase in complex with NADPH
50% identity, 94% coverage: 9:312/323 of query aligns to 5:304/305 of 8ccjA
- binding flavin-adenine dinucleotide: I8 (≠ L12), G9 (= G13), S10 (= S14), G11 (= G15), P12 (= P16), A13 (= A17), E32 (≠ Q36), G33 (= G37), Q35 (= Q39), G38 (= G42), A39 (≠ Q43), L40 (= L44), T43 (= T47), N48 (= N52), D80 (≠ T84), V81 (≠ I85), M109 (≠ T114), G110 (= G115), T131 (≠ A136), C135 (= C140), G274 (= G282), D275 (= D283), R282 (= R290), Q283 (= Q291), A284 (= A292), A287 (= A295)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G150 (= G155), G151 (= G156), D152 (≠ N157), S153 (≠ T158), E156 (= E161), H172 (= H177), R173 (= R178), R174 (= R179), R178 (= R183), I235 (= I243)
5w4cA Crystal structure of thioredoxin reductase from cryptococcus neoformans in complex with fad (fo conformation)
48% identity, 95% coverage: 7:314/323 of query aligns to 18:334/356 of 5w4cA
- binding calcium ion: E99 (≠ D83), E116 (vs. gap), E118 (≠ R98)
- binding flavin-adenine dinucleotide: I23 (≠ L12), G24 (= G13), S25 (= S14), P27 (= P16), G28 (≠ A17), Y46 (≠ V35), G48 (= G37), A51 (vs. gap), F54 (vs. gap), G58 (= G42), Q59 (= Q43), L60 (= L44), T63 (= T47), N68 (= N52), V101 (≠ I85), T134 (= T114), G135 (= G115), G302 (= G282), D303 (= D283), R310 (= R290), Q311 (= Q291), A312 (= A292), S315 (≠ A295)
Sites not aligning to the query:
2a87A Crystal structure of m. Tuberculosis thioredoxin reductase (see paper)
50% identity, 96% coverage: 3:312/323 of query aligns to 1:308/313 of 2a87A