SitesBLAST
Comparing WP_012050345.1 NCBI__GCF_000016765.1:WP_012050345.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
58% identity, 97% coverage: 8:429/434 of query aligns to 2:421/421 of P50457
- K267 (= K273) mutation to A: No GABA-AT activity.
P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
51% identity, 97% coverage: 7:427/434 of query aligns to 2:421/426 of P22256
- I50 (= I55) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
- GS 111:112 (≠ GA 116:117) binding pyridoxal 5'-phosphate
- E211 (= E216) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
- V241 (= V246) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
- Q242 (= Q247) binding pyridoxal 5'-phosphate
- K268 (= K273) modified: N6-(pyridoxal phosphate)lysine
- T297 (= T302) binding pyridoxal 5'-phosphate
1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
51% identity, 97% coverage: 7:427/434 of query aligns to 1:420/425 of 1sffA
- active site: V18 (= V24), Y137 (≠ F143), E205 (= E211), D238 (= D244), Q241 (= Q247), K267 (= K273), T296 (= T302), R397 (= R404)
- binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (= Q84), G110 (= G116), S111 (≠ A117), Y137 (≠ F143), H138 (= H144), R140 (= R146), E205 (= E211), D238 (= D244), V240 (= V246), Q241 (= Q247), K267 (= K273), T296 (= T302)
- binding sulfate ion: N152 (≠ L158), Y393 (≠ G400)
1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
51% identity, 97% coverage: 7:427/434 of query aligns to 1:420/425 of 1sf2A
- active site: V18 (= V24), Y137 (≠ F143), E205 (= E211), D238 (= D244), Q241 (= Q247), K267 (= K273), T296 (= T302), R397 (= R404)
- binding pyridoxal-5'-phosphate: G110 (= G116), S111 (≠ A117), Y137 (≠ F143), H138 (= H144), E205 (= E211), D238 (= D244), V240 (= V246), Q241 (= Q247), K267 (= K273)
- binding sulfate ion: N152 (≠ L158), Y393 (≠ G400)
1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
51% identity, 97% coverage: 7:427/434 of query aligns to 1:420/425 of 1szkA
- active site: V18 (= V24), Y137 (≠ F143), E205 (= E211), D238 (= D244), Q241 (= Q247), K267 (= K273), T296 (= T302), R397 (= R404)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G116), S111 (≠ A117), Y137 (≠ F143), H138 (= H144), E205 (= E211), D238 (= D244), V240 (= V246), Q241 (= Q247), K267 (= K273)
3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
47% identity, 96% coverage: 12:429/434 of query aligns to 20:437/439 of 3q8nC
- active site: V32 (= V24), Y151 (≠ F143), E221 (= E211), D254 (= D244), Q257 (= Q247), K283 (= K273), T312 (= T302), R412 (= R404)
- binding 4-oxobutanoic acid: G124 (= G116), A125 (= A117), V256 (= V246), K283 (= K273)
4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
46% identity, 97% coverage: 8:427/434 of query aligns to 19:441/444 of 4atqF
- active site: V35 (= V24), Y154 (≠ F143), E226 (= E211), D259 (= D244), Q262 (= Q247), K288 (= K273), T317 (= T302), R418 (= R404)
- binding gamma-amino-butanoic acid: M95 (≠ Q84), Y154 (≠ F143), R157 (= R146), E231 (= E216), K288 (= K273), G316 (= G301)
- binding pyridoxal-5'-phosphate: G127 (= G116), A128 (= A117), Y154 (≠ F143), H155 (= H144), D259 (= D244), V261 (= V246)
6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
45% identity, 97% coverage: 8:427/434 of query aligns to 19:435/440 of 6j2vA
- active site: L35 (≠ V24), Y154 (≠ F143), D256 (= D244), K285 (= K273)
- binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G116), A128 (= A117), Y154 (≠ F143), H155 (= H144), R157 (= R146), E223 (= E211), E228 (= E216), D256 (= D244), I258 (≠ V246), K285 (= K273), G313 (= G301), T314 (= T302)
O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
36% identity, 93% coverage: 29:431/434 of query aligns to 53:462/474 of O58478
- D251 (≠ E216) mutation to A: Loss of activity.
- K308 (= K273) mutation to A: Loss of activity.
4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
37% identity, 98% coverage: 3:426/434 of query aligns to 1:394/400 of 4addA
- active site: F136 (= F143), E188 (= E211), D221 (= D244), Q224 (= Q247), K250 (= K273), T279 (= T302), R372 (= R404)
- binding pyridoxal-5'-phosphate: G103 (= G116), A104 (= A117), F136 (= F143), H137 (= H144), D221 (= D244), V223 (= V246), K250 (= K273)
- binding n~2~-(3-carboxypropanoyl)-l-ornithine: Y16 (≠ A25), F136 (= F143), R139 (= R146)
4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
37% identity, 98% coverage: 3:426/434 of query aligns to 1:394/401 of 4adbB