SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012061601.1 NCBI__GCF_000017145.1:WP_012061601.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

3gqtC Crystal structure of glutaryl-coa dehydrogenase from burkholderia pseudomallei with fragment (1,4-dimethyl-1,2,3,4- tetrahydroquinoxalin-6-yl)methylamine (see paper)
71% identity, 99% coverage: 5:394/395 of query aligns to 1:385/385 of 3gqtC

query
sites
3gqtC

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active site: L135 (= L139), T136 (= T140), A250 (= A253), E365 (= E374), R377 (= R386)
binding 1-(1,4-dimethyl-1,2,3,4-tetrahydroquinoxalin-6-yl)methanamine: W166 (= W170), K210 (= K213), L213 (= L216), T218 (= T221), Y364 (= Y373)

3gncA Crystal structure of glutaryl-coa dehydrogenase from burkholderia pseudomallei with fragment 6421 (see paper)
71% identity, 98% coverage: 5:393/395 of query aligns to 2:380/380 of 3gncA

query
sites
3gncA

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active site: L136 (= L139), T137 (= T140), A242 (= A253), E361 (= E374), R373 (= R386)
binding 1-(1-methylethyl)-1H-benzimidazole-2-sulfonic acid: R95 (= R98), S96 (= S99), I246 (= I257), V357 (= V370), Y360 (= Y373), E361 (= E374)

3eonC 2.55a crystal structure of native glutaryl-coa dehydrogenase from burkholderia pseudomallei in complex with a small molecule (see paper)
71% identity, 98% coverage: 5:393/395 of query aligns to 1:382/382 of 3eonC

query
sites
3eonC

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active site: L135 (= L139), T136 (= T140), A244 (= A253), E363 (= E374), R375 (= R386)
binding (3,5-difluorophenyl)methanol: R94 (= R98), S95 (= S99), I248 (= I257), K322 (= K331), Y362 (= Y373)

3d6bC 2.2 a crystal structure of glutaryl-coa dehydrogenase from burkholderia pseudomallei (see paper)
70% identity, 98% coverage: 5:393/395 of query aligns to 1:377/377 of 3d6bC

query
sites
3d6bC

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Y

V

P

P

I

I

Y

F

A

E

Y

F

G

G

S

S

E

D

D

A

Q

Q

K

K

R

E

K

K

Y

Y

L

L

P

P

K

K

L

L

I

A

S

T

G

G

E

E

W

W

I

I

G

G

C

C

F

F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

A

H

G

G

S

G

D

S

P

-

A

-

G

-

M

M

K

V

T

T

R

R

A

A

I

R

R

K

T

V

E

P

G

G

Y

Y

R

S

L

L

I

S

G

G

S

S

K

K

M

M

W

W

I

I

S

T

N

N

A

S

P

P

L

I

A

A

D

D

V

V

F

F

V

V

W

W

A

A

K

K

-

L

S

D

E

E

A

D

H

G

D

R

N

D

A

E

I

I

R

R

G

G

F

F

V

I

L

L

E

E

K

K

G

G

T

C

K

K

G

G

L

L

S

S

A

A

P

P

K

A

I

I

A

H

G

G

K

K

L

V

S

G

L

L

R

R

A

A

S

S

I

I

T

T

G

G

E

E

I

I

V

V

L

L

D

D

N

E

V

A

E

F

V

V

G

P

E

E

A

N

L

I

L

L

P

P

D

H

V

V

E

K

G

G

L

L

K

R

G

G

P

P

F

F

G

T

C

C

L

L

N

N

R

S

A
|
A

R

R

Y

Y

G

G

I
|
I

S

A

W

W

G

G

A

A

L

L

G

G

A

A

E

E

F

S

C

C

W

W

H

H

A

I

A

A

R

R

Q

Q

Y

Y

G

V

L

L

D

D

R

R

K

K

Q

Q

F

-

N

-

R

-

P

P

L

L

A

A

Q

A

T

N

Q

Q

L

L

F

I

Q

Q

K

K

L

L

A

A

D

D

M

M

Q

Q

T

T

E

E

I

I

A

T

L

L

G

G

L

L

Q

Q

G

G

S

V

L

L

R

R

V

L

G

G

R

R

L

M

M

K

D

D

E

E

G

G

R

T

M

A

A

A

P

V

E

E

M

I

I

T

S

S

I

I

V

M

K

K

R

R

N

N

N

S

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

Q

L

A

A

R

R

D

D

M

M

H

L

G

G

G

-

N

-

G

-

I

-

S

-

E

-

Y

F

Q

G

V

V

M

A

R

R

H

H

M

L

L

V

N

N

L

L

E

E

T

V

V

V

N

N

T

T

Y
|
Y

E
|
E

G

G

T

T

H

H

D

D

V

I

H

H

A

A

L

L

I

I

L

L

G

G

R
|
R

A

A

Q

Q

T

T

G

G

L

I

Q

Q

A

A

active site: L135 (= L139), T136 (= T140), A247 (= A253), E358 (= E374), R370 (= R386)
binding methyl thiophene-2-carboxylate: I251 (= I257), Y357 (= Y373), E358 (= E374)

2r0nA The effect of a glu370asp mutation in glutaryl-coa dehydrogenase on proton transfer to the dienolate intermediate (see paper)
66% identity, 98% coverage: 6:394/395 of query aligns to 1:388/390 of 2r0nA

query
sites
2r0nA

Q

E

F

F

Q

D

W

W

D

Q

D

D

P

P

F

L

L

V

L

L

E

E

D

E

Q

Q

L

L

T

T

E

T

D

D

E

E

R

I

M

L

I

I

R

R

D

D

T

T

A

F

R

R

A

T

Y

Y

A

C

Q

Q

E

E

K

R

L

L

Q

M

P

P

R

R

V

I

I

L

E

L

A

A

Y

N

R

R

E

N

E

E

T

V

T

F

D

H

P

R

A

E

I

I

F

I

R

S

E

E

M

M

G

G

D

E

L

L

G

G

L

V

L

L

G

G

V

P

T

T

V

I

P

-

D

K

S

G

Y

Y

G

G

G

C

V

A

G

G

A

V

S

S

Y

S

V

V

A

A

Y

Y

G

G

L

L

V

L

A

A

R

R

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R
|
R

S
|
S

M

A

M

M

S

S

V
|
V

Q

Q

S

S

L

L

V

V

V

M

Y

H

P

P

I

I

Y

Y

A

A

Y

Y

G

G

S

S

E

E

D

E

Q

Q

K

R

R

Q

K

K

Y

Y

L

L

P

P

K

Q

L

L

I

A

S

K

G

G

E

E

W

L

I

L

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

A

S

G
|
G

S
|
S

D

D

P
|
P

A

S

G

S

M

M

K

E

T

T

R

R

A

A

I

H

-

Y

-

N

R

S

T

S

E

N

G

K

G

S

Y

Y

R

T

L

L

I

N

G

G

S

T

K

K

M

T

W
|
W

I
|
I

S
x
T

N

N

A

S

P

P

L

M

A

A

D

D

V

L

F

F

V

V

W

W

A

A

K

R

S

C

E

E

A

-

H

-

D

D

N

G

A

C

I

I

R

R

G

G

F

F

V

L

L

L

E

E

K

K

G

G

T

M

K

R

G

G

L

L

S

S

A

A

P

P

K

R

I

I

A

Q

G

G

K

K

L

F

S

S

L

L

R

R

A

A

S

S

I

A

T

T

G

G

E

M

I

I

V

I

L

M

D

D

N

G

V

V

E

E

V

V

G

P

E

E

A

N

L

V

L

L

P

P

D

G

V

A

E

S

G

S

L

L

K
x
G

G

G

P

P

F
|
F

G

G

C

C

L
|
L

N
|
N

R

N

A
|
A

R

R

Y

Y

G

G

I

I

S

A

W

W

G

G

A

V

L

L

G

G

A

A

A

S

E

E

F

F

C

C

W

L

H

H

A

T

A

A

R

R

Q

Q

Y

Y

G

A

L

L

D

D

R

R

K

M

Q

Q

F

F

N

G

R

V

P

P

L

L

A

A

Q

R

T

N

Q

Q

L

L

F

I

Q

Q

K

K

L

L

A

A

D

D

M

M

Q

L

T

T

E

E

I

I

A

T

L

L

G

G

L

L

Q

H

G

A

S

C

L

L

R

Q

V

L

G

G

R

R

L

L

M

K

D

D

E

Q

G

D

R

K

M

A

A

A

P
|
P

E

E

M

M

I

V

S

S

I

L

V

L

K

K

R

R

N

N

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

Q

Q

A

A

R

R

D

D

M

M

H

L

G

G

N

N

G

G

I

I

S

S

E

D

E

E

Y

Y

Q

H

V

V

M

I

R

R

H

H

M

A

L

M

N

N

L

L

E

E

T

A

V

V

N

N

T

T

Y
|
Y

E
|
E

G

G

T
|
T

H

H

D
|
D

V

I

H

H

A

A

L

L

I
|
I

L

L

G

G

R
|
R

A

A

Q

I

T

T

G

G

L

I

Q

Q

A

A

F

F

active site: L133 (= L139), T134 (= T140), A247 (= A253), E368 (= E374), R380 (= R386)
binding flavin-adenine dinucleotide: F131 (= F137), L133 (= L139), T134 (= T140), G139 (= G145), S140 (= S146), W166 (= W170), I167 (= I171), T168 (≠ S172), Y367 (= Y373), T370 (= T376), D372 (= D378)
binding 3-thiaglutaryl-CoA: R92 (= R98), S93 (= S99), V97 (= V103), P142 (= P148), G238 (≠ K244), F241 (= F247), L244 (= L250), N245 (= N251), P318 (= P324), Y367 (= Y373), E368 (= E374), I377 (= I383)

1sirA The crystal structure and mechanism of human glutaryl-coa dehydrogenase (see paper)
66% identity, 98% coverage: 6:394/395 of query aligns to 1:388/390 of 1sirA

query
sites
1sirA

Q

E

F

F

Q

D

W

W

D

Q

D

D

P

P

F

L

L

V

L

L

E

E

D

E

Q

Q

L

L

T

T

E

T

D

D

E

E

R

I

M

L

I

I

R

R

D

D

T

T

A

F

R

R

A

T

Y

Y

A

C

Q

Q

E

E

K

R

L

L

Q

M

P

P

R

R

V

I

I

L

E

L

A

A

Y

N

R

R

E

N

E

E

T

V

T

F

D

H

P

R

A

E

I

I

F

I

R

S

E

E

M

M

G

G

D

E

L

L

G

G

L

V

L

L

G

G

V

P

T

T

V

I

P

-

D

K

S

G

Y

Y

G

G

G

C

V

A

G

G

A

V

S

S

Y

S

V

V

A

A

Y

Y

G

G

L

L

V

L

A

A

R

R

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R

R

S
|
S

M

A

M

M

S

S

V

V

Q

Q

S

S

L

L

V

V

V

M

Y

H

P

P

I

I

Y

Y

A

A

Y

Y

G

G

S

S

E

E

D

E

Q

Q

K

R

R

Q

K

K

Y

Y

L

L

P

P

K

Q

L

L

I

A

S

K

G

G

E

E

W

L

I

L

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

A

S

G
|
G

S
|
S

D

D

P

P

A

S

G

S

M

M

K

E

T

T

R

R

A

A

I

H

-

Y

-

N

R

S

T

S

E

N

G

K

G

S

Y

Y

R

T

L

L

I

N

G

G

S

T

K

K

M

T

W
|
W

I
|
I

S
x
T

N

N

A

S

P

P

L

M

A

A

D

D

V

L

F

F

V

V

W

W

A

A

K

R

S

C

E

E

A

-

H

-

D

D

N

G

A

C

I

I

R

R

G

G

F

F

V

L

L

L

E

E

K

K

G

G

T

M

K

R

G

G

L

L

S

S

A

A

P

P

K

R

I

I

A

Q

G

G

K

K

L

F

S

S

L

L

R

R

A

A

S

S

I

A

T

T

G

G

E

M

I

I

V

I

L

M

D

D

N

G

V

V

E

E

V

V

G

P

E

E

A

N

L

V

L

L

P

P

D

G

V

A

E

S

G

S

L

L

K

G

G

G

P

P

F
|
F

G
|
G

C

C

L
|
L

N
|
N

R

N

A
|
A

R
|
R

Y

Y

G

G

I

I

S

A

W

W

G

G

A

V

L

L

G

G

A

A

A

S

E

E

F

F

C

C

W

L

H

H

A

T

A

A

R

R

Q

Q

Y

Y

G

A

L

L

D

D

R

R

K

M

Q

Q

F

F

N

G

R

V

P

P

L

L

A

A

Q

R

T

N

Q

Q

L

L

F

I

Q

Q

K

K

L

L

A

A

D

D

M

M

Q

L

T

T

E

E

I

I

A

T

L

L

G

G

L

L

Q

H

G

A

S

C

L

L

R

Q

V

L

G

G

R

R

L

L

M

K

D

D

E

Q

G

D

R

K

M

A

A

A

P
|
P

E

E

M

M

I

V

S

S

I

L

V

L

K

K

R

R

N

N

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

Q

Q

A

A

R

R

D

D

M

M

H

L

G

G

N

N

G

G

I

I

S

S

E

D

E

E

Y

Y

Q

H

V

V

M

I

R

R

H

H

M

A

L

M

N

N

L

L

E

E

T

A

V

V

N

N

T

T

Y
|
Y

E
|
E

G

G

T
|
T

H

H

D

D

V

I

H

H

A

A

L

L

I

I

L

L

G

G

R
|
R

A

A

Q

I

T

T

G

G

L

I

Q

Q

A

A

F

F

active site: L133 (= L139), T134 (= T140), A247 (= A253), E368 (= E374), R380 (= R386)
binding flavin-adenine dinucleotide: F131 (= F137), L133 (= L139), T134 (= T140), G139 (= G145), S140 (= S146), W166 (= W170), I167 (= I171), T168 (≠ S172), Y367 (= Y373), T370 (= T376)
binding s-4-nitrobutyryl-coa: S93 (= S99), S140 (= S146), F241 (= F247), G242 (= G248), L244 (= L250), N245 (= N251), R248 (= R254), P318 (= P324), Y367 (= Y373), E368 (= E374), R380 (= R386)

2r0mA The effect of a glu370asp mutation in glutaryl-coa dehydrogenase on proton transfer to the dienolate intermediate (see paper)
65% identity, 98% coverage: 6:394/395 of query aligns to 1:388/390 of 2r0mA

query
sites
2r0mA

Q

E

F

F

Q

D

W

W

D

Q

D

D

P

P

F

L

L

V

L

L

E

E

D

E

Q

Q

L

L

T

T

E

T

D

D

E

E

R

I

M

L

I

I

R

R

D

D

T

T

A

F

R

R

A

T

Y

Y

A

C

Q

Q

E

E

K

R

L

L

Q

M

P

P

R

R

V

I

I

L

E

L

A

A

Y

N

R

R

E

N

E

E

T

V

T

F

D

H

P

R

A

E

I

I

F

I

R

S

E

E

M

M

G

G

D

E

L

L

G

G

L

V

L

L

G

G

V

P

T

T

V

I

P

-

D

K

S

G

Y

Y

G

G

G

C

V

A

G

G

A

V

S

S

Y

S

V

V

A

A

Y

Y

G

G

L

L

V

L

A

A

R

R

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

Y

R

R

S

S

M

A

M

M

S

S

V

V

Q

Q

S

S

L
|
L

V

V

V

M

Y

H

P

P

I

I

Y

Y

A

A

Y

Y

G

G

S

S

E

E

D

E

Q

Q

K

R

R

Q

K

K

Y

Y

L

L

P

P

K

Q

L

L

I

A

S

K

G

G

E

E

W

L

I

L

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

N

A

S

G
|
G

S
|
S

D

D

P

P

A

S

G

S

M

M

K

E

T

T

R

R

A

A

I

H

-

Y

-

N

R

S

T

S

E

N

G

K

G

S

Y

Y

R

T

L

L

I

N

G

G

S

T

K

K

M

T

W
|
W

I
|
I

S
x
T

N

N

A

S

P

P

L

M

A

A

D

D

V

L

F

F

V

V

W

W

A

A

K

R

S

C

E

E

A

-

H

-

D

D

N

G

A

C

I

I

R

R

G

G

F

F

V

L

L

L

E

E

K

K

G

G

T

M

K

R

G

G

L

L

S

S

A

A

P

P

K

R

I

I

A

Q

G

G

K

K

L

F

S

S

L
|
L

R

R

A

A

S

S

I

A

T

T

G

G

E

M

I

I

V

I

L

M

D

D

N

G

V

V

E

E

V

V

G

P

E

E

A

N

L

V

L

L

P

P

D

G

V

A

E

S

G

S

L

L

K

G

G

G

P

P

F

F

G

G

C

C

L

L

N

N

R

N

A
|
A

R

R

Y

Y

G

G

I

I

S

A

W

W

G

G

A

V

L

L

G

G

A

A

A

S

E

E

F

F

C

C

W

L

H

H

A

T

A

A

R

R

Q

Q

Y

Y

G

A

L

L

D

D

R

R

K

M

Q

Q

F

F

N

G

R

V

P

P

L

L

A

A

Q

R

T

N

Q

Q

L

L

F

I

Q

Q

K

K

L

L

A

A

D

D

M

M

Q

L

T

T

E

E

I

I

A

T

L

L

G

G

L

L

Q

H

G

A

S

C

L

L

R

Q

V

L

G

G

R

R

L

L

M

K

D

D

E

Q

G

D

R

K

M

A

A

A

P

P

E

E

M

M

I

V

S

S

I

L

V

L

K

K

R

R

N

N

C

C

G

G

K

K

A

A

L

L

D

D

I

I

A

A

R

R

Q

Q

A

A

R

R

D

D

M

M

H

L

G

G

N

N

G

G

I

I

S

S

E

D

E

E

Y

Y

Q

H

V

V

M

I

R

R

H

H

M

A

L

M

N

N

L

L

E

E

T

A

V

V

N

N

T

T

Y
|
Y

E
x
D

G

G

T
|
T

H

H

D

D

V

I

H

H

A

A

L

L

I

I

L

L

G

G

R
|
R

A

A

Q

I

T

T

G

G

L

I

Q

Q

A

A

F

F

active site: L133 (= L139), T134 (= T140), A247 (= A253), D368 (≠ E374), R380 (= R386)
binding 4-nitrobutanoic acid: L101 (= L107), Y367 (= Y373), D368 (≠ E374)
binding flavin-adenine dinucleotide: F131 (= F137), L133 (= L139), T134 (= T140), G139 (= G145), S140 (= S146), W166 (= W170), I167 (= I171), T168 (≠ S172), L210 (= L216), Y367 (= Y373), T370 (= T376)

2ebaA Crystal structure of the putative glutaryl-coa dehydrogenase from thermus thermophilus
51% identity, 97% coverage: 11:394/395 of query aligns to 3:380/380 of 2ebaA

query
sites
2ebaA

D

D

P

F

F

Y

L

A

L

L

E

E

D

D

Q

L

L

L

T

T

E

P

D

E

E

E

R

K

M

E

I

V

R

Q

D

K

T

A

A

A

R

R

A

R

Y

F

A

L

Q

E

E

K

K

E

L

A

Q

L

P

P

R

H

V

I

I

R

E

D

A

W

Y

W

R

E

E

E

E

G

T

V

T

F

D

P

P

T

A

H

I

L

F

I

R

P

E

R

M

F

G

A

D

E

L

L

G

G

L

F

L

L

G

G

V

P

T

T

V

L

P

P

D

P

S

E

Y

Y

G

G

V

A

G

G

A

V

S

S

Y

S

V

A

A

A

Y

Y

G

G

L

L

V

I

A

C

R

Y

E

E

V

L

E

E

R

R

V

V

D

D

S

S

G

G

Y

L

R

R

S

S

M

F

M

V

S

S

V

V

Q

Q

S

S

L

L

V

V

V

M

Y

Y

P

P

I

I

Y

Y

A

A

Y

Y

G

G

S

S

E

E

D

E

Q

Q

K

K

R

R

K

E

Y

F

L

L

P

P

K

K

L

L

I

A

S

R

G

G

E

E

W

M

I

V

G

G

C

C

F

F

G

G

L
|
L

T
|
T

E

E

P

P

D

D

A
x
G

G
|
G

S
|
S

D

D

P

P

A

Y

G

G

-

N

M

M

K

K

T

T

R

R

A

A

I

R

R

R

T

D

E

T

G

-

Y

W

R

V

L

L

I

N

G

G

S

T

K

K

M

M

W
|
W

I

I

S
x
T

N

N

A

G

P

N

L

L

A

A

D

H

V

L

F

A

V

V

V

I

W

W

A

A

K

K

S

D

E

E

A

-

H

-

D

-

N

-

A

-

I

V

R

L

G

G

F

F

V

L

L

V

E

P

K

T

G

D

T

T

K

P

G

G

L

F

S

Q

A

A

P

R

K

E

I

V

A

K

G

R

K

K

L

M

S

S

L

L

R

R

A

A

S

S

I

V

T

T

G

S

E

E

I

L

V

V

L

L

D

E

N

E

V

V

E

R

V

V

G

P

E

E

E

S

A

L

L

R

L

L

P

P

D

K

V

A

E

L

G

G

L

L

K

K

G

A

P

P

F

L

G

S

C

C

L

L

N

T

R

Q

A
|
A

R

R

Y

F

G

G

I

I

S

A

W

W

G

G

A

A

L

M

G

G

A

A

A

L

E

E

F

A

C

V

W

Y

H

E

A

E

A

A

R

V

Q

A

Y

F

G

A

L

K

D

S

R
|
R

K

S

Q

T

F

F

N

G

R

E

P

P

L
|
L

A

A

Q

K

T
x
K

Q

Q

L

L

F

V

Q

Q

K

A

K

K

L

L

A

A

D

E

M

M

Q

L

T

A

E

W

I

H

A

T

L

E

G

G

L

L

Q

L

G

L

S

A

L

W

R

R

V

L

G

A

R

R

L

L

M

K

D

D

E

E

G

G

R

K

M

L

A

T

P

P

E

A

M

Q

I

V

S

S

I

L

V

A

K

K

R

R

N

Q

N

N

C

V

G

W

K

K

A

A

L

L

D

Q

I

A

A

A

R

R

Q

M

A

A

R

R

D
|
D

M
x
I

H

L

G

G

G
|
G

N

S

G

G

I

I

S

T

E

L

E

E

Y

Y

Q

H

V

A

M

I

R

R

H

H

M

M

L

L

N

N

L

L

E

E

T
|
T

V

V

N

Y

T
|
T

Y
|
Y

E
|
E

G

G

T
|
T

H

H

D

D

V

V

H

H

A

T

L

L

I

V

L

L

G

G

R
|
R

A

E

Q

I

T

T

G

G

L

L

Q

N

A

A

F

F

active site: L131 (= L139), T132 (= T140), A239 (= A253), E360 (= E374), R372 (= R386)
binding flavin-adenine dinucleotide: L131 (= L139), T132 (= T140), G136 (≠ A144), G137 (= G145), S138 (= S146), W161 (= W170), T163 (≠ S172), R265 (= R279), L272 (= L286), K275 (≠ T289), D333 (= D347), I334 (≠ M348), G337 (= G351), T355 (= T369), T358 (= T372), Y359 (= Y373), T362 (= T376)

3sf6A Crystal structure of glutaryl-coa dehydrogenase from mycobacterium smegmatis (see paper)
50% identity, 97% coverage: 10:394/395 of query aligns to 5:386/387 of 3sf6A

query
sites
3sf6A

D

D

P

L

F

I

L

G

L

I

E

N

D

A

Q

V

L

L

T

S

E

A

D

E

E

E

R

R

M

E

I

I

R

R

D

D

T

T

A

V

R

R

A

S

Y

V

A

V

Q

Q

E

R

K

R

L

I

Q

K

P

P

R

H

V

I

I

A

E

S

A

W

Y

Y

R

-

E

E

E

D

T

G

T

E

D

L

P

P

A

A

-

R

-

E

I

L

F

A

R

V

E

E

M

L

G

G

D

E

L

L

G

G

L

L

G

G

V

M

T

H

V

L

P

-

D

K

S

G

Y

Y

G

G

G

C

V

A

G

G

A

M

S

S

Y

A

V

V

A

A

Y

Y

G

G

L

L

V

A

A

C

R

L

E

E

V

L

E

E

R

A

V

G

D

D

S

S

G

G

Y

I

R

R

S

S

M

L

M

V

S

S

V

V

Q

Q

S

G

S

S

L

L

V

A

V

M

Y

Y

P

A

I

I

Y

H

A

A

Y

F

G

G

S

S

E

D

D

E

Q

Q

K

K

R

D

K

Q

Y

W

L

L

P

P

K

D

L

M

I

A

S

S

G

G

E

H

W

R

I

I

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

D

A

H

G
|
G

S
|
S

D

D

P

P

A

A

G

G

M

M

K

R

T

T

R

R

A

A

I

T

R

R

T

S

E

G

G

D

Y

W

R

I

L

L

I

T

G

G

S

T

K

K

M

M

W
|
W

I
|
I

S
x
T

N

N

A

G

P

S

L

V

A

A

D

D

V

V

F

A

V

V

W

W

A

A

K

R

S

T

E

-

A

-

H

-

D

D

N

E

A

G

I

I

R

R

G

G

F

F

V

V

L

V

E

P

K

T

G

D

T

T

K

P

G

G

L

F

S

T

A

A

P

N

K

T

I

I

A

K

G

S

K

K

L

M

S

S

L

L

R

R

A

A

S

S

I

V

T

T

G

S

E

E

I

L

V

V

L

L

D

D

N

G

V

V

E

R

V

L

G

P

E

D

E

S

A

A

L

R

L

L

P

P

D

G

V

A

E

T

G

S

L

L

K

G

G

A

P

P

F

L

G

R

C

C

L

L

N

N

R

E

A
|
A

R

R

Y

F

G

G

I

I

S

V

W

F

G

G

A

A

L

L

G

G

A

A

E

R

F

D

C

C

W

L

H

E

A

T

A

A

R

L

Q

A

Y

Y

G

A

L

C

D

S

R

R

K

E

Q

Q

F

F

N

D

R

R

P

P

L

I

A

G

Q

G

T

F

Q

Q

L

L

F

T

Q

Q

K

Q

K

K

L

L

A

A

D

D

M

M

Q

T

T

L

E

E

I

Y

A

G

L

K

G

G

L

F

Q

L

G

L

S

A

L

L

R

H

V

L

G

G

R

R

L

Q

M

K

D

D

E

A

G

G

R

E

M

L

A

A

P

P

E

E

M

Q

I

V

S

S

I

L

V

G

K

K

R

L

N

N

C

V

G

R

K

E

A

A

L

I

D

E

I

I

A

A

R

R

Q

T

A

A

R

R

D

T

M

V

H

L

G

G

G

A

N

S

G

G

I

I

S

T

E

G

E

E

Y

Y

Q

P

V

V

M

M

R

R

H

H

M

A

L

N

N

N

L

L

E

E

T
x
S

V

V

N

L

T
|
T

Y
|
Y

E
|
E

G

G

T
|
T

H

S

D
x
E

V
x
M

H

H

A

T

L

L

I

I

L

I

G

G

R
x
Q

A

A

Q

L

T

T

G

G

L

V

Q

G

A

A

F

F

active site: L134 (= L139), T135 (= T140), A245 (= A253), E366 (= E374), Q378 (≠ R386)
binding dihydroflavine-adenine dinucleotide: F132 (= F137), L134 (= L139), T135 (= T140), G140 (= G145), S141 (= S146), W165 (= W170), I166 (= I171), T167 (≠ S172), S361 (≠ T369), T364 (= T372), Y365 (= Y373), T368 (= T376), E370 (≠ D378), M371 (≠ V379)

3swoA Crystal structure of a glutaryl-coa dehydrogenase from mycobacterium smegmatis in complex with fadh2 (see paper)
47% identity, 99% coverage: 4:394/395 of query aligns to 1:387/388 of 3swoA

query
sites
3swoA

K

K

S

S

Q

T

F

Y

Q

A

W

P

D

L

D

E

P

L

F

F

L

D

L

T

E

D

D

R

Q

L

L

L

T

D

E

Q

D

D

E

E

R

R

M

D

I

I

R

A

D

A

T

T

A

V

R

R

A

Q

Y

F

A

V

Q

D

E

T

K

R

L

L

Q

K

P

P

R

N

V

V

I

-

E

E

A

G

Y

W

R

F

E

E

E

S

T

A

T

T

D

L

P

P

A

S

-

E

I

L

F

A

R

K

E

E

M

F

G

G

D

N

L

L

G

G

L

V

L

L

G

G

V

M

T

H

V

L

P

-

D

Q

S

G

Y

Y

G

G

G

C

V

A

G

G

A

T

S

N

Y

A

V

V

A

S

Y

Y

G

G

L

L

V

A

A

C

R

M

E

E

V

L

E

E

R

A

V

G

D

D

S

S

G

G

Y

F

R

R

S

S

M

F

M

V

S

S

V

V

Q

Q

S

G

S

S

L

L

V

S

V

M

Y

F

P

S

I

I

Y

Y

A

R

Y

Y

G

G

S

S

E

E

D

E

Q

Q

K

K

R

N

K

E

Y

W

L

L

P

P

K

R

L

L

I

A

S

A

G

G

E

D

W

A

I

I

G

G

C

C

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

D

A

F

G
|
G

S
|
S

D

N

P

P

A

A

G

G

M

M

K

R

T

T

R

R

A

A

I

R

R

R

T

D

E

G

G

S

G

D

Y

W

R

I

L

L

I

N

G

G

S

T

K

K

M

M

W
|
W

I
|
I

S
x
T

N

N

A

G

P

N

L

L

A

A

D

D

V

V

F

A

V

T

V

V

W

W

A

A

K

Q

S

T

E

-

A

-

H

-

D

D

N

D

A

G

I

I

R

R

G

G

F

F

V

L

L

V

E

P

K

T

G

D

T

T

K

P

G

G

L

F

S

T

A

A

P

N

K

E

I

I

A

H

G

R

K

K

L

L

S

S

L

L

R

R

A

A

S

S

I

V

T

T

G

S

E

E

I

L

V

V

L

L

D

D

N

N

V

V

E

R

V

L

G

P

E

A

E

S

A

A

L

Q

L

L

P

P

D

L

V

A

E

E

G

G

L

L

K

S

G

A

P

P

F

L

G

S

C

C

L

L

N

N

R

E

A
|
A

R

R

Y

F

G

G

I

I

S

V

W

F

G

G

A

A

L

L

G

G

A

A

E

R

F

D

C

S

W

L

H

E

A

T

R

I

Q

A

Y

Y

G

T

L

Q

D

S

R
|
R

K

E

Q
x
V

F
|
F

N

D

R

K

P

P

L
|
L

A

S

Q

N

T
x
Y

Q

Q

L

L

F

T

Q

Q

K

E

K

K

L

L

A

A

D

N

M

M

Q

T

T

V

E

E

I

L

A

G

L

K

G

G

L

M

Q

L

G

L

S

A

L

I

R

H

V

L

G

G

R

R

L

I

M

K

D

D

E

A

G

E

R

G

M

V

A

R

P

P

E

E

M

Q

I

I

S

S

I

L

V

G

K

K

R

L

N

N

C

V

G

R

K

E

A

A

L

I

D

A

I

I

A

A

R

R

Q

E

A

C

R

R

D
x
T

M
x
L

H

L

G

G

G
|
G

N

S

G

G

I
|
I

S

T

E

L

E

E

Y

Y

Q

S

V

P

M

L

R

R

H

H

M

A

L

N

N

N

L

L

E

E

T

S

V

V

N

L

T
|
T

Y
|
Y

E
|
E

G

G

T
|
T

H

S

D
x
E

V
x
M

H

H

A

L

L

L

I

S

L

I

G

G

R
x
K

A

A

Q

L

T

T

G

G

L

K

Q

A

A

A

F

F

active site: L135 (= L139), T136 (= T140), A246 (= A253), E367 (= E374), K379 (≠ R386)
binding dihydroflavine-adenine dinucleotide: F133 (= F137), L135 (= L139), T136 (= T140), G141 (= G145), S142 (= S146), W166 (= W170), I167 (= I171), T168 (≠ S172), R272 (= R279), V274 (≠ Q281), F275 (= F282), L279 (= L286), Y282 (≠ T289), T340 (≠ D347), L341 (≠ M348), G344 (= G351), I347 (= I354), T365 (= T372), Y366 (= Y373), T369 (= T376), E371 (≠ D378), M372 (≠ V379)

2ix6A Short chain specific acyl-coa oxidase from arabidopsis thaliana, acx4 (see paper)
35% identity, 97% coverage: 10:394/395 of query aligns to 30:412/416 of 2ix6A

query
sites
2ix6A

D

S

D

D

P

Y

F

Y

L

H

L

F

E

N

D

D

Q

L

L

L

T

T

E

P

D

E

E

E

R

Q

M

A

I

I

R

R

D

K

T

K

A

V

R

R

A

E

Y

C

A

M

Q

E

E

K

K

E

L

V

Q

A

P

P

R

I

V

M

I

T

E

E

A

Y

Y

W

R

E

E

K

E

A

T

E

T

F

D

P

P

F

A

H

I

I

F

T

R

P

E

K

M

L

G

G

D

A

L

M

G

G

L

V

L

A

G

G

V

G

T

S

V

I

P

-

D

K

S

G

Y

Y

G

G

G

C

V

P

G

G

A

L

S

S

Y

I

V

T

A

A

Y

N

G

A

L

I

V

A

A

T

R

A

E

E

V

I

E

A

R

R

V

V

D

D

S

A

G

S

Y

C

R

S

S

T

M

F

M

I

S

L

V

V

Q

H

S

S

L

L

V

G

V

M

Y

L

P

T

I

I

Y

A

A

L

Y

C

G

G

S

S

E

E

D

A

Q

Q

K

K

R

E

K

K

Y

Y

L

L

P

P

K

S

L

L

I

A

S

Q

G

L

E

N

W

T

I

V

G

A

C

C

F

W

G

A

L
|
L

T
|
T

E

E

P

P

D

D

A

N

G
|
G

S
|
S

D

D

P

A

A

S

G

G

M

L

K

G

T

T

R

T

A

A

I

T

R

K

T

V

E

E

G

G

Y

W

R

K

L

I

I

N

G

G

S

Q

K

K

M

R

W
|
W

I

I

S

G

N

N

A

S

P

T

L

F

A

A

D

D

V

L

F

L

V

I

W

F

A

A

K

R

S

N

E

T

A

T

H

-

D

T

N

N

A

Q

I

I

R

N

G

G

F

F

V

I

L

V

E

K

K

K

G

D

T

A

K

P

G

G

L

L

S

K

A

A

P

T

K

K

I

I

A

P

G

N

K

K

L

I

S

G

L

L

R

R

A

M

S

V

I

Q

T
x
N

G

G

E

D

I

I

V

L

L

L

D

Q

N

N

V

V

E

F

V

V

G

P

E

D

E

E

A

D

L

R

L

L

P

P

D

G

V

V

E

N

G

S

L

F

K

Q

G

D

P

T

F

S

G

K

C

V

L

L

N

A

R

V

A
x
S

R

R

Y

V

G

M

I

V

S

A

W

W

G

Q

A

P

L

I

G

G

A

I

A

S

E

M

F

G

C

I

W

Y

H

D

A

M

A

C

R

H

Q

R

Y

Y

G

L

L

K

D

E

R
|
R

K

K

Q

Q

F
|
F

N

G

R

A

P

P

L
|
L

A

A

Q

A

T
x
F

Q

Q

L

L

F
x
N

Q

Q

K

Q

K

K

L

L

A

V

D

Q

M

M

Q

L

T

G

E

N

I

V

A

Q

L

A

G

M

L

F

Q

L

G

M

S

G

L

W

R

R

V

L

G

C

R

K

L

L

M

Y

D

E

E

T

G

G

R

Q

M

M

A

T

P

P

E

G

M

Q

I

A

S

S

I

L

V

G

K

K

R

A

N

W

N

I

C

S

G

S

K

K

A

A

L

R

D

E

I

T

A

A

R

S

Q

L

A

G

R

R

D
x
E

M
x
L

H

L

G

G

G
|
G

N

N

G

G

I
|
I

S

L

E

A

E

D

Y

F

Q

L

V

V

M

A

R

K

H

A

M

F

L

C

N

D

L

L

E

E

T

P

V

I

N

Y

T

T

Y
|
Y

E
|
E

G

G

T
|
T

H

Y

D
|
D

V

I

H

N

A

T

L

L

I

V

L

T

G

G

R
|
R

A

E

Q

V

T

T

G

G

L

I

Q

A

A

S

F

F

active site: L158 (= L139), T159 (= T140), S271 (≠ A253), E392 (= E374), R404 (= R386)
binding flavin-adenine dinucleotide: T159 (= T140), G164 (= G145), S165 (= S146), W189 (= W170), N239 (≠ T221), R297 (= R279), F300 (= F282), L304 (= L286), F307 (≠ T289), N310 (≠ F292), E365 (≠ D347), L366 (≠ M348), G369 (= G351), I372 (= I354), Y391 (= Y373), T394 (= T376), D396 (= D378)

2ix5A Short chain specific acyl-coa oxidase from arabidopsis thaliana, acx4 in complex with acetoacetyl-coa (see paper)
35% identity, 97% coverage: 10:394/395 of query aligns to 30:412/415 of 2ix5A

query
sites
2ix5A

D

S

D

D

P

Y

F

Y

L

H

L

F

E

N

D

D

Q

L

L

L

T

T

E

P

D

E

E

E

R

Q

M

A

I

I

R

R

D

K

T

K

A

V

R

R

A

E

Y

C

A

M

Q

E

E

K

K

E

L

V

Q

A

P

P

R

I

V

M

I

T

E

E

A

Y

Y

W

R

E

E

K

E

A

T

E

T

F

D

P

P

F

A

H

I

I

F

T

R

P

E

K

M

L

G

G

D

A

L

M

G

G

L

V

L

A

G

G

V

G

T

S

V

I

P

-

D

K

S

G

Y

Y

G

G

G

C

V

P

G

G

A

L

S

S

Y

I

V

T

A

A

Y

N

G

A

L

I

V

A

A

T

R

A

E

E

V

I

E

A

R

R

V

V

D

D

S

A

G

S

Y

C

R

S

S

T

M

F

M

I

S

L

V

V

Q

H

S

S

L

L

V

G

V

M

Y

L

P

T

I

I

Y

A

A

L

Y

C

G

G

S

S

E

E

D

A

Q

Q

K

K

R

E

K

K

Y

Y

L

L

P

P

K

S

L

L

I

A

S

Q

G

L

E

N

W

T

I

V

G

A

C

C

F

W

G

A

L
|
L

T
|
T

E

E

P

P

D

D

A

N

G
|
G

S
|
S

D

D

P
x
A

A
x
S

G

G

M

L

K

G

T

T

R

T

A

A

I

T

R

K

T

V

E

E

G

G

Y

W

R

K

L

I

I

N

G

G

S

Q

K

K

M

R

W
|
W

I

I

S

G

N

N

A

S

P

T

L

F

A

A

D

D

V

L

F

L

V

I

W

F

A

A

K

R

S

N

E

T

A

T

H

-

D

T

N

N

A

Q

I

I

R

N

G

G

F

F

V

I

L

V

E

K

K

K

G

D

T

A

K

P

G

G

L

L

S

K

A

A

P

T

K

K

I

I

A

P

G

N

K

K

L

I

S

G

L

L

R

R

A

M

S

V

I

Q

T
x
N

G

G

E

D

I

I

V

L

L

L

D

Q

N

N

V

V

E

F

V

V

G

P

E

D

E

E

A

D

L

R

L

L

P

P

D

G

V

V

E

N

G

S

L
x
F

K

Q

G

D

P

T

F

S

G

K

C

V

L
|
L

N

A

R

V

A
x
S

R
|
R

Y

V

G

M

I

V

S

A

W

W

G

Q

A

P

L

I

G

G

A

I

A

S

E

M

F

G

C

I

W

Y

H

D

A

M

A

C

R

H

Q

R

Y

Y

G

L

L

K

D

E

R
|
R

K

K

Q

Q

F
|
F

N

G

R

A

P

P

L
|
L

A

A

Q

A

T
x
F

Q

Q

L
|
L

F
x
N

Q

Q

K

Q

K

K

L

L

A

V

D

Q

M

M

Q

L

T

G

E

N

I

V

A

Q

L

A

G

M

L

F

Q

L

G

M

S

G

L

W

R

R

V

L

G

C

R

K

L

L

M

Y

D

E

E

T

G

G

R

Q

M

M

A

T

P

P

E

G

M

Q

I

A

S

S

I

L

V

G

K

K

R

A

N

W

N

I

C

S

G

S

K

K

A

A

L

R

D

E

I

T

A

A

R

S

Q

L

A

G

R

R

D
x
E

M
x
L

H

L

G
|
G

N

N

G

G

I

I

S

L

E

A

E

D

Y

F

Q

L

V

V

M

A

R

K

H

A

M

F

L

C

N

D

L

L

E

E

T

P

V

I

N

Y

T

T

Y
|
Y

E
|
E

G
|
G

T
|
T

H

Y

D
|
D

V
x
I

H

N

A

T

L

L

I

V

L

T

G

G

R
|
R

A

E

Q

V

T

T

G

G

L

I

Q

A

A

S

F

F

active site: L158 (= L139), T159 (= T140), S271 (≠ A253), E392 (= E374), R404 (= R386)
binding acetoacetyl-coenzyme a: S165 (= S146), A167 (≠ P148), S168 (≠ A149), F261 (≠ L243), L268 (= L250), R272 (= R254), E392 (= E374), G393 (= G375), R404 (= R386)
binding flavin-adenine dinucleotide: L158 (= L139), T159 (= T140), G164 (= G145), S165 (= S146), W189 (= W170), N239 (≠ T221), R297 (= R279), F300 (= F282), L304 (= L286), F307 (≠ T289), L309 (= L291), N310 (≠ F292), E365 (≠ D347), L366 (≠ M348), G368 (= G350), G369 (= G351), Y391 (= Y373), T394 (= T376), D396 (= D378), I397 (≠ V379)

Q96329 Acyl-coenzyme A oxidase 4, peroxisomal; AOX 4; G6p; Short-chain acyl-CoA oxidase; AtCX4; AtG6; SAOX; EC 1.3.3.6 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
35% identity, 97% coverage: 10:394/395 of query aligns to 46:428/436 of Q96329

query
sites
Q96329

D

S

D

D

P

Y

F

Y

L

H

L

F

E

N

D

D

Q

L

L

L

T

T

E

P

D

E

E

E

R

Q

M

A

I

I

R

R

D

K

T

K

A

V

R

R

A

E

Y

C

A

M

Q

E

E

K

K

E

L

V

Q

A

P

P

R

I

V

M

I

T

E

E

A

Y

Y

W

R

E

E

K

E

A

T

E

T

F

D

P

P

F

A

H

I

I

F

T

R

P

E

K

M

L

G

G

D

A

L

M

G

G

L

V

L

A

G

G

V

G

T

S

V

I

P

-

D

K

S

G

Y

Y

G

G

G

C

V

P

G

G

A

L

S

S

Y

I

V

T

A

A

Y

N

G

A

L

I

V

A

A

T

R

A

E

E

V

I

E

A

R

R

V

V

D

D

S

A

G

S

Y

C

R

S

S

T

M

F

M

I

S

L

V

V

Q

H

S

S

L

L

V

G

V

M

Y

L

P

T

I

I

Y

A

A

L

Y

C

G

G

S

S

E

E

D

A

Q

Q

K

K

R

E

K

K

Y

Y

L

L

P

P

K

S

L

L

I

A

S

Q

G

L

E

N

W

T

I

V

G

A

C

C

F
x
W

G
x
A

L
|
L

T
|
T

E

E

P

P

D

D

A

N

G

G

S
|
S

D
|
D

P
x
A

A
x
S

G

G

M

L

K

G

T

T

R

T

A

A

I

T

R

K

T

V

E

E

G

G

Y

W

R

K

L

I

I

N

G

G

S

Q

K

K

M

R

W

W

I

I

S
x
G

N

N

A

S

P

T

L

F

A

A

D

D

V

L

F

L

V

I

W

F

A

A

K

R

S

N

E

T

A

T

H

-

D

T

N

N

A

Q

I

I

R

N

G

G

F

F

V

I

L

V

E

K

K

K

G

D

T

A

K

P

G

G

L

L

S

K

A

A

P

T

K

K

I

I

A

P

G

N

K

K

L

I

S

G

L

L

R

R

A

M

S

V

I

Q

T

N

G

G

E

D

I

I

V

L

L

L

D

Q

N

N

V

V

E

F

V

V

G

P

E

D

E

E

A

D

L

R

L

L

P

P

D

G

V

V

E

N

G

S

L

F

K

Q

G

D

P

T

F

S

G

K

C

V

L

L

N

A

R

V

A

S

R
|
R

Y

V

G

M

I

V

S

A

W

W

G

Q

A

P

L

I

G

G

A

I

A

S

E

M

F

G

C

I

W

Y

H

D

A

M

A

C

R

H

Q

R

Y

Y

G

L

L

K

D

E

R
|
R

K
|
K

Q
|
Q

F

F

N

G

R

A

P

P

L

L

A

A

Q

A

T

F

Q
|
Q

L

L

F

N

Q

Q

K

Q

K

K

L

L

A

V

D

Q

M

M

Q

L

T

G

E

N

I

V

A

Q

L

A

G

M

L

F

Q

L

G

M

S

G

L

W

R

R

V

L

G

C

R

K

L

L

M

Y

D

E

E

T

G

G

R

Q

M

M

A

T

P

P

E

G

M

Q

I

A

S

S

I

L

V

G

K

K

R

A

N

W

N

I

C

S

G

S

K

K

A

A

L

R

D

E

I

T

A

A

R

S

Q

L

A

G

R

R

D
x
E

M

L

H

L

G

G

G
|
G

N

N

G

G

I

I

S

L

E

A

E

D

Y

F

Q

L

V

V

M

A

R

K

H

A

M

F

L

C

N

D

L

L

E

E

T

P

V

I

N

Y

T

T

Y

Y

E

E

G
|
G

T
|
T

H
x
Y

D
|
D

V

I

H

N

A

T

L

L

I

V

L

T

G

G

R

R

A

E

Q

V

T

T

G

G

L

I

Q

A

A

S

F
|
F

WALT 172:175 (≠ FGLT 137:140) binding FAD
S181 (= S146) binding FAD
SDAS 181:184 (≠ SDPA 146:149) binding an acyl-CoA
G207 (≠ S172) binding FAD
R288 (= R254) binding an acyl-CoA
RKQ 313:315 (= RKQ 279:281) binding FAD
Q324 (= Q290) binding FAD
E381 (≠ D347) binding FAD
G385 (= G351) binding FAD
G409 (= G375) binding an acyl-CoA
TYD 410:412 (≠ THD 376:378) binding FAD
F428 (= F394) binding FAD

4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
35% identity, 94% coverage: 15:387/395 of query aligns to 1:376/380 of 4l1fA

query
sites
4l1fA

L

M

E

D

D

F

Q

N

L
|
L

T

T

E

E

D

D

E

Q

R
x
Q

M

M

I

I

R

K

D

D

T

M

A

A

R

A

A

E

Y

F

A

A

Q

E

E

K

K

F

L

L

Q

A

P

P

R

T

V

V

I

E

E

E

A

R

Y

D

R

K

E

A

E

H

T

I

T

W

D

D

P

R

A

K

I

L

F

I

R

D

E

K

M

M

G

G

D

E

L

A

G

G

L

F

L

C

G

G

V

I

T

C

V

F

P

P

D

E

S

E

Y

Y

G

G

V

M

G

G

A

L

S

D

Y

V

V

L

A

S

Y

Y

G

I

L

L

V

A

A

V

R

E

E

E

V

L

E

S

R

K

V

V

D

D

S

D

G

G

Y

T

R

G

S

I

M

T

M

L

S

S

V

A

Q

N

S

V

S

S

L

L

V

C

V

A

Y

T

P

P

I

I

Y

Y

A

M

Y

F

G

G

S

T

E

E

D

E

Q

Q

K

K

R

Q

K

K

Y

Y

L

L

P

A

K

P

L

I

I

A

S

E

G

G

E

T

W

H

I

V

G

G

C

A

F
|
F

G

G

L
|
L

T
|
T

E

E

P

P

D

S

A

A

G
|
G

S
x
T

D

D

P

A

A

S

G

A

M

Q

K

Q

T

T

R

T

A

A

I

V

R

L

T

K

E

G

G

D

G

K

Y

Y

R

I

L

L

I

N

G

G

S

S

K

K

M

I

W
x
F

I
|
I

S
x
T

N

N

A

G

P

K

L

E

A

A

D

D

V

T

F

Y

V

V

W

F

A

A

K

M

S

T

E

D

A

K

H

S

D

Q

-

G

-

V

N
x
H

A

G

I

I

R

S

G

A

F

F

V

I

L

L

E

E

K

K

G

G

T

M

K

P

G

G

L

F

S

R

A

F

P

G

K

K

I

I

A

E

G

D

K

K

L

M

S

G

L

G

R

H

A

T

S

S

I

I

T

T

G

A

E

E

I

L

V

I

L

F

D

E

N

D

V

C

E

E

V

V

G

P

E

K

E

E

A

N

L

L

-

G

P

K

D

E

V

G

E

E

G

G

L
x
F

K

K

G

I

P

A

F
x
M

G
x
E

C

T

L
|
L

N
x
D

R

G

A
x
G

R
|
R

Y

I

G

G

I

V

S

A

W

A

G

Q

A

A

L

L

G

G

A

I

A

A

E

E

F

G

C

A

W

L

H

A

A

A

R

V

Q

K

Y

Y

G

S

L

K

D

E

R
|
R

K

E

Q
|
Q

F
|
F

N

G

R

R

P

S

L
x
I

A

S

Q

K

T
x
F

Q

Q

L

A

F
x
L

Q

Q

K

F

K

M

L

M

A

A

D

D

M

M

Q

A

T

T

E

K

I

I

A

E

L

A

G

A

L

R

Q

Y

G

L

S

V

L

Y

R

H

V

A

G

A

R

M

L

L

M

K

D

N

E

E

G

G

R

K

M

P

A

Y

P

S

E

E

M

A

I

A

S

A

I

M

V

A

K

K

R

C

N

F

N

A

C

S

G

D

K

V

A

A

L

M

D

E

I

V

A

T

R

T

Q

D

A

A

R

V

D
x
Q

M
x
I

H

F

G

G

G
|
G

N

Y

G

G

I

Y

S

T

E

V

E

D

Y

Y

Q

P

V

A

M

E

R

R

H

Y

M

M

L

R

N

N

L

A

E

K

T
x
I

V

T

N

Q

T

I

Y
|
Y

E
|
E

G
|
G

T
|
T

H

N

D
x
Q

V

V

H

M

A

R

L

I

I

V

L

T

G

S

R
|
R

A

A

active site: L125 (= L139), T126 (= T140), G242 (≠ A253), E363 (= E374), R375 (= R386)
binding coenzyme a persulfide: T132 (≠ S146), H179 (≠ N191), F232 (≠ L243), M236 (≠ F247), E237 (≠ G248), L239 (= L250), D240 (≠ N251), R243 (= R254), Y362 (= Y373), E363 (= E374), G364 (= G375), R375 (= R386)
binding flavin-adenine dinucleotide: F123 (= F137), L125 (= L139), T126 (= T140), G131 (= G145), T132 (≠ S146), F156 (≠ W170), I157 (= I171), T158 (≠ S172), R268 (= R279), Q270 (= Q281), F271 (= F282), I275 (≠ L286), F278 (≠ T289), L281 (≠ F292), Q336 (≠ D347), I337 (≠ M348), G340 (= G351), I358 (≠ T369), Y362 (= Y373), T365 (= T376), Q367 (≠ D378)
binding 1,3-propandiol: L5 (= L19), Q10 (≠ R24)

5lnxD Crystal structure of mmgc, an acyl-coa dehydrogenase from bacillus subtilis.
34% identity, 93% coverage: 20:386/395 of query aligns to 3:370/374 of 5lnxD

query
sites
5lnxD

T

V

E

Q

D

E

E

Q

R

V

M

M

I

M

R

R

D

K

T

M

A

V

R

R

A

D

Y

F

A

A

Q

R

E

K

K

E

L

I

Q

A

P

P

R

-

V

-

I

-

E

A

A

A

Y

E

R

I

E

M

E

E

T

K

T

T

D

D

P

E

A

F

I

P

F

F

-

Q

-

L

-

I

R

K

E

K

M

M

G

G

D

K

L

H

G

G

L

L

L

M

G

G

V

I

T

P

V

V

P

P

D

E

S

Q

Y

Y

G

G

V

A

G

G

A

A

S

D

Y

V

V

V

A

S

Y

Y

G

I

L

L

V

A

A

I

R

H

E

E

V

I

E

S

R

R

V

I

D

S

S

A

G

A

Y

V

R

G

S

V

M

I

M

L

S

S

V

V

Q

H

S

T

S

S

L

V

V

G

V

T

Y

N

P

P

I

I

Y

L

A

Y

Y

F

G

G

S

N

E

E

D

E

Q

Q

K

K

R

M

K

K

Y

Y

L

I

P

P

K

N

L

L

I

A

S

S

G

G

E

D

W

H

I

L

G

G

C

A

F

F

G

A

L
|
L

T
|
T

E

E

P

P

D

H

A

S

G
|
G

S
|
S

D

D

P

A

A

G

G

S

M

L

K

R

T

T

R

T

A

A

I

I

R

K

T

K

E

N

G

G

G

K

Y

Y

R

L

L

L

I

N

G

G

S

S

K

K

M

I

W
x
F

I

I

S
x
T

N

N

A

G

P

G

L

A

A

A

D

D

V

I

F

Y

V

I

V

T

W

F

A

A

K

L

S

T

E

A

A

P

H

D

D

Q

-

G

-

R

N

H

A

G

I

I

R

S

G

A

F

F

V

I

L

V

E

E

K

K

G

N

T

T

K

P

G

G

L

F

S

T

A

V

P

G

K

K

I

K

A

E

G

R

K

K

L

L

S

G

L

L

R

Y

A

G

S

S

I

N

T

T

G

T

E

E

I

L

V

I

L

F

D

D

N

N

V

A

E

E

V

V

G

P

E

E

E

A

A

N

L

L

-

G

P

K

D

E

V

G

E

D

G

G

L

F

K

H

G

I

P

A

F

M

G

A

C

N

L

L

N

N

R

V

A
x
G

R

R

Y

I

G

G

I

I

S

A

W

A

G

Q

A

A

L

L

G

G

A

I

A

A

E

E

F

A

C

A

W

L

H

E

A

H

A

A

R

V

Q

D

Y

Y

G

A

L

K

D

Q

R
|
R

K

V

Q
|
Q

F
|
F

N

G

R

R

P

P

L
x
I

A

A

Q

A

T
x
N

Q

Q

L

G

F
x
I

Q

S

K

F

K

K

L

L

A

A

D

D

M

M

Q

A

T

T

E

-

I

-

A

-

L

-

G

-

L

-

Q

-

G

-

S

-

L

-

R

-

V

-

G

-

R

R

L

A

M

E

D

A

E

A

G

R

R

H

M

L

A

V

P

Y

E

H

M

A

I

A

S

D

I

L

V

H

K

N

R

R

N

L

N

N

C

C

G

G

K

K

A

E

L

A

D

S

I

M

A

A

R

K

Q

Q

-

F

-

A

-

S

-

D

-

A

-

V

-

K

-

A

-

L

-

D

A

A

R

V

D
x
Q

M
x
I

H

Y

G

G

G
|
G

N

Y

G

G

I

Y

S

M

E

K

E

D

Y

Y

Q

P

V

V

M

E

R

R

H

L

M

L

L

R

N

D

L

A

E

K

T

V

V

T

N

Q

T

I

Y
|
Y

E
|
E

G

G

T
|
T

H

N

D
x
E

V

I

H

Q

A

R

L

L

I

I

L

I

G

S

R
x
K

active site: L122 (= L139), T123 (= T140), G239 (≠ A253), E358 (= E374), K370 (≠ R386)
binding flavin-adenine dinucleotide: L122 (= L139), T123 (= T140), G128 (= G145), S129 (= S146), F153 (≠ W170), T155 (≠ S172), R265 (= R279), Q267 (= Q281), F268 (= F282), I272 (≠ L286), N275 (≠ T289), I278 (≠ F292), Q331 (≠ D347), I332 (≠ M348), G335 (= G351), Y357 (= Y373), T360 (= T376), E362 (≠ D378)

4n5fA Crystal structure of a putative acyl-coa dehydrogenase with bound fadh2 from burkholderia cenocepacia j2315
36% identity, 94% coverage: 15:386/395 of query aligns to 2:376/378 of 4n5fA

query
sites
4n5fA

L

M

E

D

D

E

Q

L

L

Y

T

T

E

E

D

D

E

Q

R

R

M

M

I

I

R

R

D

D

T

A

A

A

R

R

A

A

Y

F

A

A

Q

T

E

E

K

M

L

L

Q

A

P

P

R

N

V

A

I

A

E

Q

A

W

Y

D

R

H

E

D

E

A

T

H

T

L

D

P

P

D

A

A

I

I

F

V

R

A

E

Q

M

L

G

G

D

E

L

L

G

G

L

L

G

G

V

M

T

I

V

V

P

P

D

Q

S

E

Y

L

G

G

V

S

G

Y

A

T

S

D

Y

Y

V

V

A

A

Y

Y

G

A

L

L

V

A

A

M

R

E

E

E

V

V

E

A

R

A

V

G

D

D

S

A

G

A

Y

C

R

A

S

T

M

M

S

S

V

V

Q

H

S

N

S

S

L

V

V

G

V

C

Y

G

P

P

I

I

Y

L

A

G

Y

F

G

G

S

T

E

P

D

A

Q

Q

K

K

R

D

K

R

Y

W

L

L

P

A

K

D

L

M

I

A

S

A

G

G

E

R

W

V

I

I

G

G

C

A

F

F

G

C

L
|
L

T
|
T

E

E

P

P

D

H

A

A

G
|
G

S
|
S

D

E

P

A

A

N

G

N

M

L

K

R

T

T

R

R

A

A

I

E

R

L

T

R

E

D

G

G

G

Q

Y

W

R

V

L

L

I

N

G

G

S

A

K

K

M

Q

W
x
F

I

V

S
x
T

N

N

A

G

P

Q

L

R

A

A

D

G

V

V

F

A

V

I

V

V

W

F

A

A

K

M

S

T

-

D

-

P

E

E

A

A

H

G

D

K

N

R

A

G

I

I

R

S

G

A

F

F

V

L

L

V

E

P

K

T

G

D

T

T

K

P

G

G

L

F

S

I

A

V

P

G

K

K

I

P

A

E

G

K

K

K

L

M

S

G

L

I

R

R

A

A

S

S

I

D

T
|
T

G

C

E

P

I

I

V

T

L

F

D

E

N

N

V

C

E

A

V

I

G

P

E

E

E

D

A

N

L

L

P

G

D

N

V

R

-

G

E

E

G

G

L

L

K

K

G

I

P

A

F

L

G

S

C

N

L

L

N

E

R

G

A
x
G

R

R

Y

I

G

G

I

I

S

A

W

A

G

Q

A

A

L

L

G

G

A

I

A

A

E

R

F

A

C

A

W

F

H

D

A

K

A

A

R

R

Q

R

Y

Y

G

A

L

G

D

E

R

R

K

V

Q

Q

F

F

N

G

R

K

P

P

L

I

A

A

Q

E

T

H

Q

Q

L

A

F

I

Q

Q

K

Q

K

K

L

L

A

A

D

D

M

M

Q

A

T

V

E

Q

I

I

A

N

-

A

-

R

-

L

L

V

G

H

L

H

Q

A

G

A

S

K

L

L

R

R

V

T

G

A

R

G

L

L

-

P

-

C

M

L

D

S

E

E

G

A

R

S

M

Q

A

A

P

K

E

L

M

F

I

A

S

S

I

E

V

M

K

A

R

E

N

R

N

V

C

C

G

S

K

D

A

A

L

I

D

Q

I

I

A

-

R

-

Q

-

A

-

R

-

D

-

M

-

H

H

G

G

N

Y

G

G

I

Y

S

L

E

V

E

D

Y

Y

Q

E

V

V

M

E

R

R

H

H

M

Y

L

R

N

D

L

A

E

R

T

I

V

T

N

Q

T

I

Y
|
Y

E
|
E

G

G

T
|
T

H

S

D
x
E

V

V

H

Q

A

R

L
x
M

I

V

L

I

G

A

R
|
R

active site: L126 (= L139), T127 (= T140), G243 (≠ A253), E364 (= E374), R376 (= R386)
binding dihydroflavine-adenine dinucleotide: L126 (= L139), T127 (= T140), G132 (= G145), S133 (= S146), F157 (≠ W170), T159 (≠ S172), T210 (= T221), Y363 (= Y373), T366 (= T376), E368 (≠ D378), M372 (≠ L382)

2dvlA Crystal structure of project tt0160 from thermus thermophilus hb8
36% identity, 93% coverage: 19:386/395 of query aligns to 1:366/370 of 2dvlA

query
sites
2dvlA

L

L

T

T

E

Q

D

E

E

Q

R

R

M

L

I

V

R

L

D

D

T

A

A

V

R

R

A

R

Y

V

A

A

Q

R

E

E

K

V

L

L

Q

Y

P

P

R

L

V

A

I

P

E

E

A

Y

Y

D

R

R

E

K

E

A

T

E

T

Y

D

P

P

W

A

P

I

Q

F

L

R

K

E

A

M

L

G

A

D

E

L

L

G

G

L

L

G

G

V

M

T

T

V

T

P

P

D

E

S

E

Y

W

G

G

V

V

G

G

A

L

S

D

Y

S

V

V

A

T

Y

W

G

A

L

L

V

A

A

L

R

E

E

E

V

L

E

A

R

A

V

A

D

D

S

P

G

S

Y

V

R

A

S

V

M

I

M

V

S

S

V

V

Q

T

S

S

S

G

L

L

V

P

V

Q

Y

Y

P

M

I

L

Y

L

A

R

Y

F

G

G

S

S

E

E

D

A

Q

Q

K

K

R

R

K

R

Y

Y

L

L

P

V

K

P

L

L

I

A

S

R

G

G

E

E

W

W

I

I

G

G

C

A

F

F

G

C

L
|
L

T
|
T

E

E

P

P

D

Q

A

A

G
|
G

S
|
S

D

D

P

A

A

K

G

S

M

L

K

R

T

A

R

E

A

A

I

R

R

R

T

V

E

K

G

G

Y

F

R

V

L

L

I

N

G

G

S

V

K

K

M

S

W
|
W

I
|
I

S
x
T

N

S

A

A

P

G

L

H

A

A

D

H

V

L

F

Y

V

V

W

M

A

A

K

R

S

T

E

E

A

-

H

-

D

-

N

K

A

G

I

I

R

S

G

A

F

F

V

L

L

V

E

E

K

K

G

G

T

T

K

P

G

G

L

L

S

S

A

F

P

G

K

R

I

P

A

E

G

E

K

K

L

M

S

G

L

L

R

H

A

A

S

A

I

H

T

T

G

A

E

E

I

V

V

R

L

L

D

E

N

E

V

V

E

F

V

V

G

P

E

E

A

N

L

L

-

G

P

E

D

E

V

G

E

R

G

G

L

L

K

A

G

Y

P

A

F

L

G

A

C

G

L

L

N

D

R

S

A
x
G

R

R

Y

V

G

G

I

V

S

A

W

A

G

Q

A

A

L

V

G

G

A

I

A

A

E

R

F

G

C

A

W

F

H

E

A

I

A

A

R

K

Q

A

Y

Y

G

A

L

E

D

E

R

R

K

E

Q

Q

F

F

N

G

R

K

P

K

L

L

A

K

Q

E

T

H

Q

Q

L

A

F

I

Q

A

K

F

K

K

L

I

A

A

D

D

M

M

Q

H

T

V

E

K

I

I

A

A

L

A

G

A

L

R

Q

A

G

L

S

V

L

L

R

E

V

A

G

A

R

R

L

K

M

K

D

D

E

R

G

G

R

E

M

R

A

F

P

T

E

L

M

E

I

A

S

S

I

A

V

A

K

K

R

L

N

F

N

A

C

S

G

A

K

A

A

A

L

V

D

E

I

V

A

T

R

R

Q

E

A

A

R

V

D

Q

M

V

H

L

G

G

N

Y

G

G

I

Y

S

H

E

R

E

D

Y

Y

Q

R

V

V

M

E

R

R

H

Y

M

Y

L

R

N

D

L

A

E

K

T

V

V

T

N

E

T

I

Y

Y

E
|
E

G

G

T
|
T

H

S

D
x
E

V

I

H

Q

A

R

L

L

I

V

L

I

G

A

R
|
R

active site: L121 (= L139), T122 (= T140), G233 (≠ A253), E354 (= E374), R366 (= R386)
binding flavin-adenine dinucleotide: L121 (= L139), T122 (= T140), G127 (= G145), S128 (= S146), W152 (= W170), I153 (= I171), T154 (≠ S172), T356 (= T376), E358 (≠ D378)

5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
32% identity, 91% coverage: 24:384/395 of query aligns to 9:372/378 of 5ol2F

query
sites
5ol2F

R

Q

M

M

I

L

R

K

D

E

T

L

A

Y

R

V

A

S

Y

F

A

A

Q

E

E

N

K

E

L

V

Q

K

P

P

R

L

V

A

I

T

E
|
E

A

L

Y

D

R

E

E
|
E

E

E

T
x
R

T

F

D

P

P

Y

A

E

I

T

F

V

R

E

E

K

M

M

G

A

D

K

L

A

G

G

L

M

G

G

V

I

T

P

V

Y

P

P

D

K

S

E

Y

Y

G

G

V

E

G

G

A

G

S

D

Y

T

V

V

A

G

Y

Y

G

I

L

M

V

A

A

V

R

E

E

E

V

L

E

S

R

R

V

V

D

C

S

G

G

T

Y

T

R

G

S

V

M

I

M

L

S

S

V

A

Q

H

S

T

S

S

L

L

V

G

V

S

Y

W

P

P

I

I

Y

Y

A

Q

Y

Y

G

G

S

N

E

E

D

E

Q

Q

K

K

R

Q

K

K

Y

F

L

L

P

R

K

P

L

L

I

A

S

S

G

G

E

E

W

K

I

L

G

G

C

A

F
|
F

G

G

L
|
L

T
|
T

E

E

P
|
P

D

N

A

A

G

G

S
x
T

D

D

P

A

A

S

G

G

M

Q

K

Q

T

T

R

T

A

A

I

V

R

L

T

D

E

G

G

D

G

E

Y

Y

R

I

L

L

I

N

G

G

S

S

K

K

M

I

W
x
F

I
|
I

S
x
T

N

N

A

A

P

I

L

A

A

G

D

D

V

I

F

Y

V

V

W

M

A

A

K

M

S

T

E

D

A

K

H

S

-

K

-

G

D

N

N

K

A

G

I

I

R

S

G

A

F

F

V

I

L

V

E

E

K

K

G

G

T

T

K

P

G

G

L

F

S

S

A

F

P

G

K

V

I

K

A
x
E

G

K

K

K

L

M

S

G

L

I

R

R

A

G

S

S

I

A

T

T

G

S

E

E

I

L

V

I

L

F

D

E

N

D

V

C

E

R

V

I

G

P

E

K

E

E

A

N

L

L

-

G

P

K

D

E

V

G

E

Q

G

G

L

F

K

K

G

I

P

A

F

M

G

S

C

T

L
|
L

N

D

R

G

A
x
G

R
|
R

Y

I

G

G

I

I

S

A

W

A

G

Q

A

A

L

L

G

G

A

L

A

A

E

Q

F

G

C

A

W

L

H

D

A

E

A

T

R

V

Q

K

Y

Y

G

V

L

K

D

E

R
|
R

K

V

Q

Q

F
|
F

N

G

R

R

P

P

L
|
L

A

S

Q

K

T
x
F

Q

Q

L

N

F

T

Q

Q

K

F

K

Q

L

L

A

A

D

D

M

M

Q

E

T

V

E

K

I

V

A

Q

L

A

G

A

L

R

Q

H

G

L

S

V

L

Y

R

Q

V

A

G

A

R

I

L

N

M

K

D

D

E

L

G

G

R

K

M

P

A

Y

P

G

E

V

M

E

I

A

S

A

I

M

V

A

K

K

R

L

N

F

N

A

C

A

G

E

K

T

A

A

L

M

D

E

I

V

A

T

R

T

Q

K

A

A

R

V

D
x
Q

M
x
L

H

H

G
|
G

N

Y

G

G

I

Y

S

T

E

R

E

D

Y

Y

Q

P

V

V

M

E

R

R

H

M

M

M

L

R

N

D

L

A

E

K

T

I

V

T

N

E

T

I

Y
|
Y

E
|
E

G
|
G

T
|
T

H

S

D
x
E

V

V

H

Q

A

R

L

M

I

V

L

I

active site: L124 (= L139), T125 (= T140), G241 (≠ A253)
binding calcium ion: E29 (= E44), E33 (= E48), R35 (≠ T50)
binding coenzyme a persulfide: L238 (= L250), R242 (= R254), E362 (= E374), G363 (= G375)
binding flavin-adenine dinucleotide: F122 (= F137), L124 (= L139), T125 (= T140), P127 (= P142), T131 (≠ S146), F155 (≠ W170), I156 (= I171), T157 (≠ S172), E198 (≠ A211), R267 (= R279), F270 (= F282), L274 (= L286), F277 (≠ T289), Q335 (≠ D347), L336 (≠ M348), G338 (= G350), G339 (= G351), Y361 (= Y373), T364 (= T376), E366 (≠ D378)

Sites not aligning to the query:

active site: 374

2z1qB Crystal structure of acyl coa dehydrogenase
34% identity, 93% coverage: 17:384/395 of query aligns to 22:404/549 of 2z1qB

query
sites
2z1qB

D

E

Q

D

L

F

T

D

E

E

D

S

E

V

R

K

M

E

I

I

R

A

D

R

T

T

A

T

R

R

A

T

Y

F

A

V

Q

E

E

R

K

E

L

V

Q

L

P

P

R

L

V

L

-

E

-

R

I

M

E

E

A

H

Y

G

R

E

E

L

E

E

T

L

T

N

D

V

P

P

A

-

I

L

F

M

R

R

E

K

M

A

G

G

D

E

L

L

G

G

L

L

G

A

V

I

T

D

V

V

P

P

D

E

S

E

Y

Y

G

G

V

L

G

D

A

L

S

P

Y

K

V

V

A

I

Y

S

G

T

L

V

V

V

A

A

R

E

E

E

V

L

E

S

R

G

V

-

D

S

S

G

G

G

Y

F

R

S

S

V

M

T

M

Y

S

G

V

A

Q

H

S

T

S

S

L

I

V

G

V

T

Y

L

P

P

I

L

Y

V

A

Y

Y

F

G

G

S

T

E

E

D

E

Q

Q

K

K

R

R

K

K

Y

Y

L

L

P

P

K

K

L

L

I

A

S

S

G

G

E

E

W

W

I

I

G

A

C

A

F
x
Y

G

C

L
|
L

T
|
T

E

E

P

P

D

G

A

S

G
|
G

S
|
S

D

D

P

A

A

L

G

A

M

A

K

K

T

T

R

R

A

A

I

T

R

L

T

S

E

E

G

D

G

G

-

K

-

H

Y

Y

R

I

L

L

I

N

G

G

S

V

K

K

M

Q

W
|
W

I

I

S
|
S

N

N

A

A

P

G

L

F

A

A

D

H

V

L

F

F

V

T

V

V

W

F

A

A

K

K

S

V

E

D

A

G

H

E

D

H

N

-

A

-

I

F

R

T

G

A

F

F

V

L

L

V

E

E

K

R

G

D

T

T

K

P

G

G

L

L

S

S

A

F

P

G

K

P

I

E

A

E

G

K

K

K

L

M

S

G

L

I

R

K

A

A

S

S

I

S

T

T

G

R

E

Q

I

V

V

I

L

L

D

E

N

D

V

V

E

K

V

V

G

P

E

V

E

E

A

N

L

V

L

L

P

G

D

E

V

I

-

G

E

K

G

G

L

H

K

K

G

I

P

A

F

F

G

N

C

V

L

L

N

N

R

V

A
x
G

R

R

Y

Y

G

K

I

L

S

G

W

A

G

G

A

A

L

V

G

G

A

G

A

A

E

K

F

R

C

A

W

L

H

E

A

L

A

S

R

A

Q

Q

Y

Y

G

A

L

T

D

Q

R
|
R

K

V

Q

Q

F
|
F

N

G

R

R

P

P

L
x
I

A

G

Q

R

T
x
F

Q

G

L

L

F
x
I

Q

Q

K

Q

K

K

L

L

A

G

D

E

M

M

Q

A

T

S

E

R

I

I

A

Y

L

A

G

A

L

E

Q

S

G

A

S

V

L

Y

R

R

V

T

G

V

R

G

L

L

M

I

D

D

E

E

-

A

-

L

G

G

R

K

M

K

A

G

P

P

E

E

M

A

I

V

-

M

-

A

-

G

-

I

-

E

-

Y

-

A

-

V

-

E

-

A

S

S

I

I

V

I

K

K

R

V

N

L

N

G

C

S

G

-

K

E

A

V

L

L

D

D

-

Y

I

V

A

V

R

D

Q

E

A

G

R

V

D

Q

M

I

H
|
H

G
|
G

G

G

N

Y

G

G

I

Y

S

S

E

Q

E

E

Y

Y

Q

P

V

I

M

E

R

R

H

A

M

Y

L

R

N

D

L

A

E

R

T

I

V

N

N

R

T

I

Y
x
F

E
|
E

G

G

T

T

H

N

D

E

V
x
I

H

N

A

R

L

L

I

L

L

I

active site: L144 (= L139), T145 (= T140), G259 (≠ A253), E394 (= E374)
binding flavin-adenine dinucleotide: Y142 (≠ F137), L144 (= L139), T145 (= T140), G150 (= G145), S151 (= S146), W177 (= W170), S179 (= S172), R285 (= R279), F288 (= F282), I292 (≠ L286), F295 (≠ T289), I298 (≠ F292), H369 (= H349), G370 (= G350), F393 (≠ Y373), I399 (≠ V379)

Sites not aligning to the query:

active site: 406
binding flavin-adenine dinucleotide: 448

1bucA Three-dimensional structure of butyryl-coa dehydrogenase from megasphaera elsdenii (see paper)
33% identity, 94% coverage: 15:387/395 of query aligns to 1:380/383 of 1bucA

query
sites
1bucA

L

M

E

D

D

F

Q

N

L

L

T

T

E

D

D

I

E

Q

R

Q

M

D

I

F

R

L

D

K

T

L

A

A

R

H

A

D

Y

F

A

G

Q

E

E

K

K

K

L

L

Q

A

P

P

R

T

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A

active site: L128 (= L139), T129 (= T140), G246 (≠ A253), E367 (= E374), G379 (≠ R386)
binding acetoacetyl-coenzyme a: L96 (= L107), F126 (= F137), G134 (= G145), T135 (≠ S146), T162 (≠ S172), N182 (≠ D190), H183 (≠ N191), F236 (≠ L243), M240 (≠ F247), M241 (≠ G248), L243 (= L250), D244 (≠ N251), T317 (≠ P324), Y366 (= Y373), E367 (= E374), G368 (= G375)
binding flavin-adenine dinucleotide: F126 (= F137), L128 (= L139), T129 (= T140), G134 (= G145), T135 (≠ S146), F160 (≠ W170), T162 (≠ S172), Y366 (= Y373), T369 (= T376), E371 (≠ D378), M375 (≠ L382)

Query Sequence

>WP_012061601.1 NCBI__GCF_000017145.1:WP_012061601.1
MAGKSQFQWDDPFLLEDQLTEDERMIRDTARAYAQEKLQPRVIEAYREETTDPAIFREMG
DLGLLGVTVPDSYGGVGASYVAYGLVAREVERVDSGYRSMMSVQSSLVVYPIYAYGSEDQ
KRKYLPKLISGEWIGCFGLTEPDAGSDPAGMKTRAIRTEGGYRLIGSKMWISNAPLADVF
VVWAKSEAHDNAIRGFVLEKGTKGLSAPKIAGKLSLRASITGEIVLDNVEVGEEALLPDV
EGLKGPFGCLNRARYGISWGALGAAEFCWHAARQYGLDRKQFNRPLAQTQLFQKKLADMQ
TEIALGLQGSLRVGRLMDEGRMAPEMISIVKRNNCGKALDIARQARDMHGGNGISEEYQV
MRHMLNLETVNTYEGTHDVHALILGRAQTGLQAFF

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory