SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012061607.1 NCBI__GCF_000017145.1:WP_012061607.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P50457 4-aminobutyrate aminotransferase PuuE; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; EC 2.6.1.19 from Escherichia coli (strain K12) (see paper)
61% identity, 97% coverage: 7:417/422 of query aligns to 9:419/421 of P50457

query
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P50457

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K267 (= K265) mutation to A: No GABA-AT activity.

P22256 4-aminobutyrate aminotransferase GabT; 5-aminovalerate transaminase; GABA aminotransferase; GABA-AT; Gamma-amino-N-butyrate transaminase; GABA transaminase; Glutamate:succinic semialdehyde transaminase; L-AIBAT; EC 2.6.1.19; EC 2.6.1.48 from Escherichia coli (strain K12) (see 2 papers)
57% identity, 99% coverage: 4:421/422 of query aligns to 7:425/426 of P22256

query
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P22256

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I50 (= I47) mutation to Q: 3-fold decrease in catalytic activity and 12-fold decrease in affinity for GABA.
GS 111:112 (≠ GA 108:109) binding pyridoxal 5'-phosphate
E211 (= E208) mutation to S: 100-fold decrease in catalytic activity and 15-fold decrease in affinity for GABA.
V241 (= V238) mutation to A: 25-fold decrease in catalytic activity and 5-fold decrease in affinity for GABA.
Q242 (= Q239) binding pyridoxal 5'-phosphate
K268 (= K265) modified: N6-(pyridoxal phosphate)lysine
T297 (= T294) binding pyridoxal 5'-phosphate

1sffA Structure of gamma-aminobutyrate aminotransferase complex with aminooxyacetate (see paper)
57% identity, 99% coverage: 4:421/422 of query aligns to 6:424/425 of 1sffA

query
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1sffA

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active site: V18 (= V16), Y137 (≠ F135), E205 (= E203), D238 (= D236), Q241 (= Q239), K267 (= K265), T296 (= T294), R397 (= R394)
binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Q78 (= Q76), G110 (= G108), S111 (≠ A109), Y137 (≠ F135), H138 (= H136), R140 (= R138), E205 (= E203), D238 (= D236), V240 (= V238), Q241 (= Q239), K267 (= K265), T296 (= T294)
binding sulfate ion: N152 (≠ V150), Y393 (≠ G390)

1sf2A Structure of e. Coli gamma-aminobutyrate aminotransferase (see paper)
57% identity, 99% coverage: 4:421/422 of query aligns to 6:424/425 of 1sf2A

query
sites
1sf2A

L

L

T

M

D

Q

R

R

K

R

N

S

A

Q

A

A

I

I

S

P

R

R

G

G

V
|
V

G

G

M

Q

T

I

T

H

Q

P

I

I

Y

F

A

A

E

D

R

R

A

A

E

E

N

N

A

C

E

R

I

V

W

W

D

D

K

V

E

E

G

G

N

R

R

E

Y

Y

I

L

D

D

F

F

A

A

S

G

G

G

I

I

A

A

V

V

V

L

N

N

T

T

G

G

H

H

R

L

H

H

P

P

K

K

V

V

I

V

A

A

V

V

K

E

A

A

Q

Q

L

L

D

K

R

K

F

L

T

S

H

H

T

T

C

C

H

F

Q

Q

V

V

V

L

P

A

Y

Y

E

E

N

P

Y

Y

V

L

H

E

L

L

A

C

E

E

R

I

L

M

N

N

A

Q

I

K

V

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

I

L

F

L

V

V

T

T

G
|
G

A
x
S

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

Q

S

A

G

I

T

V

I

A

A

F

F

G

S

G

G

G

A

F
x
Y

H
|
H

G

G

R

R

T

T

F

H

M

Y

G

T

M

L

A

A

L

L

T

T

G

G

K

K

V

V

V
x
N

P

P

Y

Y

K

S

V

A

G

G

F

M

G

G

A

L

M

M

P

P

A

G

D

H

V

V

F

Y

H

R

A

A

P

L

F

Y

P

P

V

C

E

P

L

L

H

H

G

G

V

I

S

S

V

E

E

D

Q

D

S

A

L

I

A

A

A

S

L

I

K

H

K

R

L

I

F

F

A

K

A

N

D

D

V

A

D

A

P

P

N

E

R

D

V

I

A

A

I

I

I

V

I

I

E
|
E

P

P

V

V

Q

Q

G

G

E

E

G

G

F

F

Y

Y

P

A

V

S

P

S

T

P

A

A

F

F

M

M

K

Q

A

R

L

L

R

R

E

A

I

L

C

C

D

D

Q

E

N

H

G

G

I

I

L

M

L

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

A

A

G

R

R

T

T

G

G

K

T

L

L

L

F

A

A

M

M

E

E

H

Q

H

M

G

G

V

V

A

A

P

P

D

D

L

L

T

T

M

F

A

A

K
|
K

S

S

L

I

A

A

G

G

F

F

P

P

L

L

A

A

A

G

V

V

T

T

G

G

R

R

A

A

E

E

I

V

M

M

D

D

A

A

P

V

G

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

G

A

G

G

N

N

P

P

L

I

G

A

I

C

A

V

A

A

H

L

A

E

V

V

L

L

D

K

V

V

I

F

A

E

E

Q

E

E

N

N

L

L

C

L

E

Q

R

K

A

A

N

N

Q

D

L

L

G

G

N

Q

R

K

L

L

K

K

Q

D

R

G

L

L

A

L

A

A

I

I

R

A

E

E

K

K

A

H

P

P

E

E

I

I

V

G

D

D

I

V

R

R

G

G

P

L

G

G

F

A

M

M

N

I

A

A

V

I

E

E

-

L

F

F

N

E

D

D

V

G

K

D

T

H

N

N

V

K

P

P

S

D

A

A

E

K

F

L

A

T

N

A

K

E

V

I

R

V

L

A

L

R

A

A

L

R

E

D

K

K

G

G

L

L

I

I

L

L

T

S

C

C

G

G

V

P

H

Y

G
x
Y

N

N

V

V

I

L

R
|
R

F

I

L

L

A

V

P

P

I

L

T

T

I

I

Q

E

D

D

D

A

V

Q

F

I

A

R

E

Q

A

G

L

L

D

E

I

I

L

I

E

S

S

Q

S

C

I

F

L

D

E

E

A

A

R

K

active site: V18 (= V16), Y137 (≠ F135), E205 (= E203), D238 (= D236), Q241 (= Q239), K267 (= K265), T296 (= T294), R397 (= R394)
binding pyridoxal-5'-phosphate: G110 (= G108), S111 (≠ A109), Y137 (≠ F135), H138 (= H136), E205 (= E203), D238 (= D236), V240 (= V238), Q241 (= Q239), K267 (= K265)
binding sulfate ion: N152 (≠ V150), Y393 (≠ G390)

1szkA The structure of gamma-aminobutyrate aminotransferase mutant: e211s (see paper)
57% identity, 99% coverage: 4:421/422 of query aligns to 6:424/425 of 1szkA

query
sites
1szkA

L

L

T

M

D

Q

R

R

K

R

N

S

A

Q

A

A

I

I

S

P

R

R

G

G

V
|
V

G

G

M

Q

T

I

T

H

Q

P

I

I

Y

F

A

A

E

D

R

R

A

A

E

E

N

N

A

C

E

R

I

V

W

W

D

D

K

V

E

E

G

G

N

R

R

E

Y

Y

I

L

D

D

F

F

A

A

S

G

G

G

I

I

A

A

V

V

V

L

N

N

T

T

G

G

H

H

R

L

H

H

P

P

K

K

V

V

I

V

A

A

V

V

K

E

A

A

Q

Q

L

L

D

K

R

K

F

L

T

S

H

H

T

T

C

C

H

F

Q

Q

V

V

V

L

P

A

Y

Y

E

E

N

P

Y

Y

V

L

H

E

L

L

A

C

E

E

R

I

L

M

N

N

A

Q

I

K

V

V

P

P

G

G

D

D

F

F

A

A

K

K

T

T

I

L

F

L

V

V

T

T

G
|
G

A
x
S

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

T

T

G

K

R

R

Q

S

A

G

I

T

V

I

A

A

F

F

G

S

G

G

G

A

F
x
Y

H
|
H

G

G

R

R

T

T

F

H

M

Y

G

T

M

L

A

A

L

L

T

T

G

G

K

K

V

V

V

N

P

P

Y

Y

K

S

V

A

G

G

F

M

G

G

A

L

M

M

P

P

A

G

D

H

V

V

F

Y

H

R

A

A

P

L

F

Y

P

P

V

C

E

P

L

L

H

H

G

G

V

I

S

S

V

E

E

D

Q

D

S

A

L

I

A

A

A

S

L

I

K

H

K

R

L

I

F

F

A

K

A

N

D

D

V

A

D

A

P

P

N

E

R

D

V

I

A

A

I

I

I

V

I

I

E
|
E

P

P

V

V

Q

Q

G

G

E

S

G

G

F

F

Y

Y

P

A

V

S

P

S

T

P

A

A

F

F

M

M

K

Q

A

R

L

L

R

R

E

A

I

L

C

C

D

D

Q

E

N

H

G

G

I

I

L

M

L

L

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

A

A

G

R

R

T

T

G

G

K

T

L

L

L

F

A

A

M

M

E

E

H

Q

H

M

G

G

V

V

A

A

P

P

D

D

L

L

T

T

M

F

A

A

K
|
K

S

S

L

I

A

A

G

G

F

F

P

P

L

L

A

A

A

G

V

V

T

T

G

G

R

R

A

A

E

E

I

V

M

M

D

D

A

A

P

V

G

A

P

P

G

G

L

L

G

G

T
|
T

Y

Y

G

A

G

G

N

N

P

P

L

I

G

A

I

C

A

V

A

A

H

L

A

E

V

V

L

L

D

K

V

V

I

F

A

E

E

Q

E

E

N

N

L

L

C

L

E

Q

R

K

A

A

N

N

Q

D

L

L

G

G

N

Q

R

K

L

L

K

K

Q

D

R

G

L

L

A

L

A

A

I

I

R

A

E

E

K

K

A

H

P

P

E

E

I

I

V

G

D

D

I

V

R

R

G

G

P

L

G

G

F

A

M

M

N

I

A

A

V

I

E

E

-

L

F

F

N

E

D

D

V

G

K

D

T

H

N

N

V

K

P

P

S

D

A

A

E

K

F

L

A

T

N

A

K

E

V

I

R

V

L

A

L

R

A

A

L

R

E

D

K

K

G

G

L

L

I

I

L

L

T

S

C

C

G

G

V

P

H

Y

G

Y

N

N

V

V

I

L

R
|
R

F

I

L

L

A

V

P

P

I

L

T

T

I

I

Q

E

D

D

D

A

V

Q

F

I

A

R

E

Q

A

G

L

L

D

E

I

I

L

I

E

S

S

Q

S

C

I

F

L

D

E

E

A

A

R

K

active site: V18 (= V16), Y137 (≠ F135), E205 (= E203), D238 (= D236), Q241 (= Q239), K267 (= K265), T296 (= T294), R397 (= R394)
binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G110 (= G108), S111 (≠ A109), Y137 (≠ F135), H138 (= H136), E205 (= E203), D238 (= D236), V240 (= V238), Q241 (= Q239), K267 (= K265)

3q8nC Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis (see paper)
49% identity, 99% coverage: 4:421/422 of query aligns to 20:439/439 of 3q8nC

query
sites
3q8nC

L

L

T

Q

D

A

R

R

K

K

N

Q

A

S

A

A

I

V

S

A

R

A

G

G

V
|
V

G

G

M

V

T

T

T

L

Q

P

I

V

Y

Y

A

V

E

V

R

A

A

A

E

G

N

G

A

G

E

V

I

L

W

A

D

D

K

A

E

D

G

G

N

N

R

Q

Y

L

I

I

D

D

F

F

A

G

S

S

G

G

I

I

A

A

V

V

V

T

N

T

T

V

G

G

H

N

R

S

H

A

P

P

K

A

V

V

I

V

A

D

A

A

V

V

K

T

A

Q

Q

Q

L

V

D

A

R

A

F

F

T

T

H

H

T

T

C

C

H

F

Q

M

V

V

V

T

P

P

Y

Y

E

E

N

G

Y

Y

V

V

H

K

L

V

A

A

E

E

R

H

L

L

N

N

A

R

I

L

V

T

P

P

G

G

D

D

F

H

A

E

K

K

K

R

T

T

I

A

F

L

V

F

T

N

T

S

G
|
G

A
|
A

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

I

A

A

R

R

A

A

A

Y

T

T

G

R

R

R

Q

Q

A

A

I

V

V

V

A

V

F

F

G

D

G

H

G

A

F
x
Y

H

H

G

G

R

R

T

T

F

N

M

L

G

T

M

M

A

A

L

M

T

T

G

A

K

K

V

N

V

Q

P

P

Y

Y

K

K

V

H

G

G

F

F

G

G

A

P

M

F

P

A

A

N

D

E

V

V

F

Y

H

R

A

V

P

P

-

T

-

S

F

Y

P

P

V

F

E

R

L

D

H

G

G

E

V

T

S

D

V

G

E

A

Q

A

S

A

L

A

A

A

A

H

L

A

K

L

K

D

L

L

F

I

A

N

A

K

D

Q

V

V

D

G

P

A

N

D

R

N

V

V

A

A

I

V

I

I

E
|
E

P

P

V

V

Q

H

G

G

E

E

G

G

F

F

Y

V

P

V

V

P

P

A

T

P

A

G

F

F

M

L

K

G

A

A

L

L

R

Q

E

K

I

W

C

C

D

T

Q

D

N

N

G

G

I

A

L

V

L

F

I

V

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

T

G

G

F

F

A

A

R

R

T

T

G

G

K

A

L

L

L

F

A

A

M

C

E

E

H

H

H

E

G

N

V

V

A

V

P

P

D

D

L

L

T

I

T

V

M

T

A

A

K
|
K

S

G

L

I

A

A

G

G

F

L

P

P

L

L

A

S

A

A

V

V

T

T

G

G

R

R

A

A

E

E

I

I

M

M

D

D

A

G

P

P

G

Q

P

S

G

G

L

L

G

G

T
|
T

Y

Y

G

G

N

N

P

P

L

L

G

A

I

C

A

A

H

L

A

A

V

V

L

I

D

D

V

T

I

I

A

E

E

R

E

E

N

N

L

L

C

V

E

A

R

R

A

A

N

R

Q

A

L

I

G

G

N

E

R

T

L

M

K

L

Q

S

R

R

L

L

A

G

A

A

I

L

R

A

E

A

K

A

A

D

P

P

E

R

I

I

V

G

D

E

I

V

R

R

G

G

P

R

G

G

F

A

M

M

N

I

A

A

V

V

E

E

F

L

N

V

D

K

V

P

K

G

T

T

N

T

V

E

P

P

S

D

A

A

E

D

F

L

A

T

N

K

K

R

V

V

R

A

L

A

A

A

L

H

E

A

K

Q

G

G

L

L

I

V

L

V

L

L

T

T

C

C

G

G

V

T

H

Y

G

G

N

N

V

V

I

L

R
|
R

F

F

L

L

A

P

P

P

I

L

T

S

I

M

Q

P

D

D

D

H

V

L

F

L

A

D

E

E

A

G

L

L

D

D

I

I

L

L

E

A

S

A

S

V

I

F

L

A

E

E

A

V

R

K

active site: V32 (= V16), Y151 (≠ F135), E221 (= E203), D254 (= D236), Q257 (= Q239), K283 (= K265), T312 (= T294), R412 (= R394)
binding 4-oxobutanoic acid: G124 (= G108), A125 (= A109), V256 (= V238), K283 (= K265)

6j2vA Gaba aminotransferase from corynebacterium glutamicum (see paper)
46% identity, 98% coverage: 3:417/422 of query aligns to 22:435/440 of 6j2vA

query
sites
6j2vA

S

A

L

L

T

D

D

E

R

R

K

R

N

Q

A

A

I

V

S

A

R

R

G

A

V
x
L

G

A

M

P

T

G

T

L

Q

P

I

G

Y

Y

A

V

E

V

R

D

A

A

E

D

N

G

A

G

E

I

I

L

W

A

D

D

K

A

E

D

G

G

N

N

R

R

Y

F

I

I

D

D

F

L

A

A

S

S

G

G

I

I

A

A

V

V

V

T

N

T

T

V

G

G

H

G

R

S

H

N

P

A

K

A

V

V

I

A

A

K

A

A

V

V

K

G

A

A

Q

A

L

A

D

A

R

R

F

F

T

T

H

H

T

T

C

C

H

F

Q

M

V

V

V

S

P

P

Y

Y

E

E

N

T

Y

Y

V

V

H

A

L

M

A

A

E

E

R

R

L

L

N

N

A

A

I

L

V

T

P

P

G

G

D

D

F

H

A

D

K

K

T

S

I

A

F

L

V

F

T

N

T

S

G
|
G

A
|
A

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

V

A

A

R

R

A

A

A

Y

T

T

G

G

R

K

Q

G

A

A

I

V

V

V

A

V

F

F

G

D

G

N

G

A

F
x
Y

H
|
H

G

G

R
|
R

T

T

F

N

M

L

G

T

M

M

A

A

L

M

T

T

G

A

K

K

V

N

V

R

P

P

Y

Y

K

K

V

S

G

G

F

F

G

G

A

P

M

L

P

A

A

A

D

D

V

V

F

Y

H

R

A

A

P

P

F

M

P

S

V

Y

E

P

L

L

H

R

-

D

G

G

V

L

S

S

V

G

E

P

Q

E

S

A

L

A

A

E

A

R

L

A

K

I

K

S

L

V

F

I

A

E

A

S

D

Q

V

V

D

G

P

A

N

E

R

N

V

L

A

A

A

C

I

V

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

Y

I

P

V

V

P

P

A

T

P

A

G

F

F

M

L

K

A

A

A

L

I

R

S

E

T

I

W

C

C

D

R

Q

E

N

N

G

D

I

V

L

V

L

F

I

I

A

A

D
|
D

E

E

V
x
I

Q

Q

T

S

G

G

F

F

A

L

R

R

T

T

G

G

K

D

L

W

L

F

A

A

M

S

E

D

H

A

H

E

G

G

V

V

A

I

P

P

D

D

L

V

T

I

T

T

M

T

A

A

K
|
K

S

G

L

I

A

A

G

G

F

M

P

P

L

L

A

S

A

A

V

V

T

T

G

G

R

R

A

A

E

E

I

I

M

M

D

D

A

A

P

P

G

G

P

P

G

G

G

A

L

L

G

G

G
|
G

T
|
T

Y

Y

G

G

N

N

P

P

L

V

G

A

I

C

A

A

H

L

A

A

V

A

L

I

D

E

V

V

I

M

A

E

E

Q

E

A

N

D

L

L

C

K

E

T

R

R

A

A

N

Q

Q

E

L

I

G

E

N

T

R

I

L

I

K

R

Q

D

R

E

L

F

A

A

A

Q

I

L

R

-

E

S

K

A

A

F

P

P

E

E

I

V

V

A

D

E

I

I

R

R

G

G

P

R

G

G

F

A

M

M

N

M

A

A

V

I

E

E

F

L

N

I

D

D

V

A

K

-

T

T

N

G

V

R

P

P

S

N

A

A

E

A

F

L

A

T

N

A

K

A

V

V

R

A

L

A

L

R

A

A

L

K

E

A

K

E

G

G

L

V

I

L

L

L

T

T

C

C

G

G

V

T

H

D

G

G

N

N

V

V

I

I

R

R

F

L

L

L

A

P

P

P

I

L

T

V

I

I

Q

A

D

E

D

D

V

T

F

L

A

R

E

D

A

G

L

L

D

Q

I

V

L

L

E

V

S

A

I

L

active site: L35 (≠ V16), Y154 (≠ F135), D256 (= D236), K285 (= K265)
binding 4-[({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)amino]butanoic acid: G127 (= G108), A128 (= A109), Y154 (≠ F135), H155 (= H136), R157 (= R138), E223 (= E203), E228 (= E208), D256 (= D236), I258 (≠ V238), K285 (= K265), G313 (= G293), T314 (= T294)

4atqF Gaba-transaminase a1r958 in complex with external aldimine plp-gaba adduct (see paper)
46% identity, 98% coverage: 3:417/422 of query aligns to 22:441/444 of 4atqF

query
sites
4atqF

S

A

L

L

T

A

D

E

R

R

K

R

N

S

A

A

A

V

I

V

S

A

R

A

G

G

V
|
V

G

A

M

S

T

G

T

V

Q

P

I

V

Y

Y

A

V

E

E

R

D

A

A

E

D

N

G

A

G

E

I

I

I

W

R

D

D

K

V

E

D

G

G

N

N

R

S

Y

F

I

I

D

D

F

L

A

G

S

S

G

G

I

I

A

A

V

V

V

T

N

S

T

V

G

G

H

A

R

S

H

D

P

P

K

A

V

V

I

V

A

A

V

V

K

Q

A

E

Q

A

L

A

D

A

R

H

F

F

T

T

H

H

T

T

C

C

H

F

Q
x
M

V

V

V

T

P

P

Y

Y

E

E

N

G

Y

Y

V

V

H

A

L

V

A

T

E

E

R

Q

L

L

N

N

A

R

I

L

V

T

P

P

G

G

D

D

F

H

A

A

K

K

K

R

T

T

I

V

F

L

V

F

T

N

T

S

G
|
G

A
|
A

E

E

A

A

V

V

E

E

N

N

A

A

V

V

K

K

I

V

A

A

R

R

A

L

A

A

T

T

G

G

R

R

Q

D

A

A

I

V

V

V

A

A

F

F

G

D

G

H

G

A

F
x
Y

H
|
H

G

G

R
|
R

T

T

F

N

M

L

G

T

M

M

A

A

L

L

T

T

G

A

K

K

V

A

V

M

P

P

Y

Y

K

K

V

T

G

N

F

F

G

G

A

P

M

F

P

A

A

P

D

E

V

V

F

Y

H

R

A

M

P

P

-

M

-

S

F

Y

P

P

V

F

E

R

L

E

H

E

G

N

V

P

S

E

V

I

E

T

Q

G

S

A

L

E

A

A

L

K

K

R

-

A

-

I

K

T

L

M

F

I

A

E

A

K

D

Q

V

I

D

G

P

G

N

D

R

Q

V

V

A

A

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

F

Y

I

P

V

V

P

P

A

T

E

A

G

F

F

M

L

K

P

A

A

L

L

R

S

E

E

I

W

C

A

D

K

Q

E

N

K

G

G

I

I

L

V

L

F

I

I

A

A

D
|
D

E

E

V
|
V

Q
|
Q

T

S

G

G

F

F

A

C

R

R

T

T

G

G

K

E

L

W

L

F

A

A

M

V

E

D

H

H

H

E

G

G

V

V

A

V

P

P

D

D

L

I

T

I

T

T

M

M

A

A

K
|
K

S

G

L

I

A

A

G

G

F

L

P

P

L

L

A

S

A

A

V

I

T

T

G

G

R

R

A

A

E

D

I

L

M

L

D

D

A

A

P

V

G

H

P

P

G

G

L

L

G

G

G
|
G

T
|
T

Y

Y

G

G

N

N

P

P

L

V

G

A

I

C

A

A

H

L

A

A

V

A

L

I

D

D

V

T

I

M

A

E

E

Q

E

H

N

D

L

L

C

N

E

G

R

R

A

A

N

R

Q

H

L

I

G

E

N

-

R

-

L

-

K

E

Q

L

R

A

L

L

A

G

A

K

I

L

R

R

E

E

K

L

A

A

P

A

E

E

-

G

-

S

-

V

I

V

V

G

D

D

I

I

R

R

G

G

P

R

G

G

F

A

M

M

N

L

A

A

V

I

E

E

F

L

N

V

D

Q

V

P

K

G

T

S

N

K

V

E

P

P

S

N

A

A

E

E

F

L

A

T

N

K

K

A

V

V

R

A

L

A

A

C

L

L

E

K

K

E

G

G

L

V

I

I

L

I

L

L

T

T

C

C

G

G

V

T

H

Y

G

G

N

N

V

V

I

I

R
|
R

F

L

L

L

A

P

P

P

I

L

T

V

I

I

Q

S

D

D

D

E

V

L

F

L

A

I

E

D

A

G

L

L

D

E

I

V

L

L

E

A

S

A

I

I

active site: V35 (= V16), Y154 (≠ F135), E226 (= E203), D259 (= D236), Q262 (= Q239), K288 (= K265), T317 (= T294), R418 (= R394)
binding gamma-amino-butanoic acid: M95 (≠ Q76), Y154 (≠ F135), R157 (= R138), E231 (= E208), K288 (= K265), G316 (= G293)
binding pyridoxal-5'-phosphate: G127 (= G108), A128 (= A109), Y154 (≠ F135), H155 (= H136), D259 (= D236), V261 (= V238)

O58478 Alanine/serine racemase; ASR; Ala/Ser racemase; EC 5.1.1.-; EC 5.1.1.1 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
36% identity, 98% coverage: 4:417/422 of query aligns to 35:458/474 of O58478

query
sites
O58478

L

L

T

I

D

E

R

R

K

E

N

K

A

K

A

V

I

L

S

S

R

T

G

G

V

I

G

G

M

V

T

K

T

L

-

F

Q

P

I

L

Y

V

A

P

E

K

R

R

A

G

E

F

N

G

A

P

E

F

I

I

W

E

D

D

K

V

E

D

G

G

N

N

R

V

Y

F

I

I

D

D

F

F

A

L

S

A

G

G

I

A

A

A

V

A

N

S

T

T

G

G

H

Y

R

A

H

H

P

P

K

K

V

L

I

V

A

K

A

A

V

V

K

K

A

E

Q

Q

L

V

D

E

R

L

F

I

T

Q

H

H

T

S

C

M

H

I

Q

G

V

Y

V

T

P

H

Y

S

E

E

N

R

Y

A

V

I

H

R

L

V

A

A

E

E

R

K

L

L

N

V

A

E

I

I

V

S

P

P

G

I

D

E

F

-

A

N

K

S

K

K

T

V

I

I

F

F

V

G

T

L

T

S

G

G

A

S

E

D

A

A

V

V

E

D

N

M

A

A

V

I

K

K

I

V

A

S

R

K

A

F

A

S

T

T

G

R

R

R

Q

P

A

W

I

I

V

L

A

A

F

F

G

I

G

G

G

A

F

Y

H

H

G

G

R

Q

T

T

F

L

M

G

G

A

M

T

A

S

L

V

T

A

G

S

K

F

V

Q

V

V

P

S

Y

Q

K

K

V

R

G

G

F

Y

G

S

A

P

M

L

P

M

A

P

D

N

V

V

F

F

H

W

A

I

P

P

F

Y

P

P

V

N

E

P

L

F

H

R

G

N

V

I

S

W

-

G

-

I

-

N

-

G

-

Y

-

E

-

P

-

D

-

E

-

L

V

I

E

N

Q

R

S

V

L

L

A

D

A

Y

L

L

K

E

K

Y

-

Y

L

V

F

F

A

S

A

H

D

V

V

V

D

P

P

P

N

D

R

E

V

V

A

A

I

L

I

F

I

A

E

E

P

P

V

I

Q

Q

G

G

E
x
D

G

A

G

G

F

I

Y

V

P

V

V

P

P

P

T

E

A

N

F

F

M

F

K

K

A

E

L

L

R

K

E

K

I

L

C

L

D

E

Q

E

N

Y

G

G

I

I

L

L

I

V

A

M

D

D

E

E

V

V

Q

Q

T

T

G

G

F

I

A

G

R

R

T

T

G

G

K

K

L

W

L

F

A

A

M

S

E

E

H

W

H

F

G

N

V

V

A

K

P

P

D

D

L

M

T

I

M

F

A

G

K
|
K

S

G

L

V

A

A

G

S

G

G

F

M

P

G

L

L

A

S

A

G

V

V

T

I

G

G

R

R

A

K

E

E

I

I

M

M

D

D

A

I

P

T

G

S

P

G

G

S

G

A

L

L

G

-

G

L

T

T

Y

P

G

A

N

N

P

P

L

V

G

I

I

S

A

A

H

E

A

A

V

T

L

L

D

E

V

I

I

I

A

E

E

E

N

N

L

L

C

L

E

K

R

N

A

A

N

L

Q

E

L

V

G

G

N

E

R

F

L

I

K

M

Q

G

R

R

L

L

A

K

A

E

I

I

R

K

E

E

K

R

A

F

P

E

E

I

I

I

V

G

D

D

I

V

R

R

G

G

P

K

G

G

F

L

M

M

N

I

A

G

V

V

E

E

F

I

N

-

D

-

V

V

K

K

T

E

N

N

-

G

V

R

P

P

S

D

A

P

E

E

F

M

A

T

N

G

K

K

V

I

R

C

L

W

L

R

A

A

L

F

E

E

K

L

G

G

L

L

I

I

L

L

L

P

T

S

C

Y

G

G

V

M

H

F

G

G

N

N

V

V

I

I

R

R

F

I

L

T

A

P

P

P

I

L

T

V

I

L

Q

T

D

K

D

E

V

V

F

A

A

E

E

K

A

A

L

L

D

E

I

I

L

I

E

E

S

R

S

A

I

I

D251 (≠ E208) mutation to A: Loss of activity.
K308 (= K265) mutation to A: Loss of activity.

O50131 Ornithine aminotransferase; Orn-AT; Ornithine delta-aminotransferase; EC 2.6.1.13 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
40% identity, 93% coverage: 25:417/422 of query aligns to 41:442/454 of O50131

query
sites
O50131

E

E

R

R

A

A

E

E

N

G

A

V

E

Y

I

W

W

I

D

D

K

V

E

D

G

G

N

N

R

V

Y

L

I

L

D

D

F

F

A

S

S

S

G

G

I

I

A

G

V

V

V

M

N

N

T

V

G

G

H

L

R

R

H

N

P

P

K

K

V

V

I

I

A

E

A

A

V

I

K

K

A

K

Q

Q

L

L

D

D

R

L

F

V

T

L

H

H

T

A

-

A

-

G

C
x
T

H
x
D

Q

Y

V

Y

V

N

P

P

Y

Y

E

Q

N

-

Y

-

V

V

H

E

L

L

A

A

E

K

R

K

L

L

N

V

A

E

I

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

I

F

F

L

V

S

T

N

T

S

G
|
G

A
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

Q

K

A

M

I

F

V

I

A

A

F

F

G

I

G

G

G

A

F

F

H

H

G

G

R

R

T

T

F

H

M

G

G

T

M

M

A

S

L

L

T

T

-

A

G

S

K

K

V

P

V

V

P

Q

Y

R

K

S

V

R

G

M

F

F

G

P

A

T

M

M

P

P

A

G

D

-

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

V

N

E

P

L

Y

H

R

-

N

-

P

-

W

G

G

V

I

S

D

V

G

E

Y

Q

E

S

N

L

P

A

D

A

E

L

L

-

I

-

N

-

R

-

V

-

I

-

D

-

Y

-

I

-

E

K

E

K

Y

L

L

F

F

A

E

A

H

D

Y

V

V

D

P

P

A

N

E

R

E

V

V

A

A

A

G

I

I

I

F

E

E

P

P

V

I

Q

Q

G

G

E

E

G

G

F

Y

Y

V

P

V

V

P

P

P

T

K

A

N

F

F

M

F

K

K

A

E

L

L

R

K

E

K

I

L

C

A

D

D

Q

K

N

H

G

G

I

I

L

L

I

I

A

D

D

D

E

E

V

V

Q
|
Q

T

M

G

G

F

M

A

G

R

R

T

T

G

G

K

R

L

M

L

W

A

A

M

I

E

E

H

H

H

F

G

D

V

I

A

V

P

P

D

D

L

I

T

V

T

T

M

V

A

A

K
|
K

S

A

L

L

A

G

G

G

F

I

P

P

L

I

A

G

A

A

V

T

T

I

G

F

R

R

A

A

E

D

I

-

M

L

D

D

A

F

P

G

G

V

P

S

G

G

G

V

L

H

G

S

G

N

T
|
T

Y

F

G

G

N

N

P

T

L

V

G

A

I

A

A

A

H

L

A

A

V

V

L

I

D

E

V

E

I

L

A

-

E

Q

E

N

N

G

L

L

C

I

E

E

R

N

A

A

N

Q

Q

K

L

L

G

E

N

P

R

L

L

F

K

R

Q

E

R

R

L

L

A

E

I

M

R

K

E

E

K

K

A

Y

P

E

E

I

I

I

V

G

D

D

I

V

R

R

G

G

P

L

G

G

F

L

M

A

N

W

A

G

V

V

E

E

F

F

-

V

N

K

D

D

V

R

K

K

T

T

N

K

V

E

P

Y

S

A

A

T

E

K

F

E

A

R

N

G

K

E

V

I

R

V

L

V

L

E

A

A

L

L

E

K

K

R

G

G

L

L

I

A

L

L

T

G

C

C

G

G

V

-

H

-

G

K

N

S

V

A

I

I

R

R

F

L

L

I

A

P

P

P

I

L

T

I

I

I

Q

S

D

E

V

E

F

A

A

K

E

M

A

G

L

L

D

D

I

I

L

F

E

E

S

E

S

A

I

I

T92 (≠ C74) mutation to V: Slightly increases the specific activity. Increases KM for L-ornithine.
D93 (≠ H75) mutation to L: Reduces the specific activity. Increases KM for L-ornithine.
G124 (= G108) binding pyridoxal 5'-phosphate
T125 (≠ A109) binding pyridoxal 5'-phosphate
Q267 (= Q239) binding pyridoxal 5'-phosphate
K293 (= K265) modified: N6-(pyridoxal phosphate)lysine
T321 (= T294) binding pyridoxal 5'-phosphate

7vo1A Structure of aminotransferase-substrate complex (see paper)
40% identity, 93% coverage: 25:417/422 of query aligns to 39:440/452 of 7vo1A

query
sites
7vo1A

E

E

R

R

A

A

E

E

N

G

A

V

E

Y

I

W

W

I

D

D

K

V

E

D

G

G

N

N

R

V

Y

L

I

L

D

D

F

F

A

S

S

S

G

G

I
|
I

A

G

V

V

V

M

N

N

T

V

G

G

H

L

R

R

H

N

P

P

K

K

V

V

I

I

A

E

A

A

V

I

K

K

A

K

Q

Q

L

L

D

D

R

L

F

V

T

L

H

H

T

A

-

A

-

G

C

T

H

D

Q

Y

V

Y

V

N

P

P

Y

Y

E

Q

N

-

Y

-

V

V

H

E

L

L

A

A

E

K

R

K

L

L

N

V

A

E

I

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

I

F

F

L

V

S

T

N

T
x
S

G
|
G

A
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

Q

K

A

M

I

F

V

I

A

A

F

F

G

I

G

G

G

A

F
|
F

H
|
H

G

G

R
|
R

T

T

F

H

M

G

G

T

M

M

A

S

L

L

T

T

-

A

G

S

K

K

V

P

V

V

P

Q

Y

R

K

S

V

R

G

M

F

F

G

P

A

T

M

M

P

P

A

G

D

-

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

V

N

E

P

L

Y

H

R

-

N

-

P

-

W

G

G

V

I

S

D

V

G

E

Y

Q

E

S

N

L

P

A

D

A

E

L

L

-

I

-

N

-

R

-

V

-

I

-

D

-

Y

-

I

-

E

K

E

K

Y

L

L

F

F

A

E

A

H

D

Y

V

V

D

P

P

A

N

E

R

E

V

V

A

A

A

G

I

I

I

F

E

E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

Y

Y

V

P

V

V

P

P

P

T

K

A

N

F

F

M

F

K

K

A

E

L

L

R

K

E

K

I

L

C

A

D

D

Q

K

N

H

G

G

I

I

L

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

T

M

G

G

F

M

A

G

R

R

T

T

G

G

K

R

L

M

L

W

A

A

M

I

E

E

H

H

H

F

G

D

V

I

A

V

P

P

D

D

L

I

T

V

T

T

M

V

A

A

K
|
K

S

A

L

L

A

G

G

G

F

I

P

P

L

I

A

G

A

A

V

T

T

I

G

F

R

R

A

A

E

D

I

-

M

L

D

D

A

F

P

G

G

V

P

S

G

G

G

V

L

H

G

S

G
x
N

T
|
T

Y

F

G

G

N

N

P

T

L

V

G

A

I

A

A

A

H

L

A

A

V

V

L

I

D

E

V

E

I

L

A

-

E

Q

E

N

N

G

L

L

C

I

E

E

R

N

A

A

N

Q

Q

K

L

L

G

E

N

P

R

L

L

F

K

R

Q

E

R

R

L

L

A

E

I

M

R

K

E

E

K

K

A

Y

P

E

E

I

I

I

V

G

D

D

I

V

R

R

G

G

P

L

G

G

F

L

M

A

N

W

A

G

V

V

E

E

F

F

-

V

N

K

D

D

V

R

K

K

T

T

N

K

V

E

P

Y

S

A

A

T

E

K

F

E

A

R

N

G

K

E

V

I

R

V

L

V

L

E

A

A

L

L

E

K

K

R

G

G

L

L

I

A

L

L

T

G

C

C

G

G

V

-

H

-

G

K

N

S

V

A

I

I

R
|
R

F

L

L

I

A

P

P

P

I

L

T

I

I

I

Q

S

D

E

V

E

F

A

A

K

E

M

A

G

L

L

D

D

I

I

L

F

E

E

S

E

S

A

I

I

binding N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-L-glutamic acid: I61 (= I47), S121 (≠ T107), G122 (= G108), T123 (≠ A109), F149 (= F135), H150 (= H136), R152 (= R138), E234 (= E208), D262 (= D236), V264 (= V238), Q265 (= Q239), K291 (= K265), N318 (≠ G293), T319 (= T294), R417 (= R394)

7vntA Structure of aminotransferase-substrate complex (see paper)
40% identity, 93% coverage: 25:417/422 of query aligns to 39:440/452 of 7vntA

query
sites
7vntA

E

E

R

R

A

A

E

E

N

G

A

V

E

Y

I

W

W

I

D

D

K

V

E

D

G

G

N

N

R

V

Y

L

I

L

D

D

F

F

A

S

S

S

G

G

I

I

A

G

V

V

V

M

N

N

T

V

G

G

H

L

R

R

H

N

P

P

K

K

V

V

I

I

A

E

A

A

V

I

K

K

A

K

Q

Q

L

L

D

D

R

L

F

V

T

L

H

H

T

A

-

A

-

G

C

T

H

D

Q

Y

V

Y

V

N

P

P

Y

Y

E

Q

N

-

Y

-

V

V

H

E

L

L

A

A

E

K

R

K

L

L

N

V

A

E

I

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

I

F

F

L

V

S

T

N

T

S

G
|
G

A
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

Q

K

A

M

I

F

V

I

A

A

F

F

G

I

G

G

G

A

F
|
F

H
|
H

G

G

R
|
R

T

T

F

H

M

G

G

T

M

M

A

S

L

L

T

T

-

A

G

S

K

K

V

P

V

V

P

Q

Y

R

K

S

V

R

G

M

F

F

G

P

A

T

M

M

P

P

A

G

D

-

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

V

N

E

P

L

Y

H

R

-

N

-

P

-

W

G

G

V

I

S

D

V

G

E

Y

Q

E

S

N

L

P

A

D

A

E

L

L

-

I

-

N

-

R

-

V

-

I

-

D

-

Y

-

I

-

E

K

E

K

Y

L

L

F

F

A

E

A

H

D

Y

V

V

D

P

P

A

N

E

R

E

V

V

A

A

A

G

I

I

I

F

E
|
E

P

P

V

I

Q

Q

G

G

E
|
E

G

G

F

Y

Y

V

P

V

V

P

P

P

T

K

A

N

F

F

M

F

K

K

A

E

L

L

R

K

E

K

I

L

C

A

D

D

Q

K

N

H

G

G

I

I

L

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

T

M

G

G

F

M

A

G

R

R

T

T

G

G

K

R

L

M

L

W

A

A

M

I

E

E

H

H

H

F

G

D

V

I

A

V

P

P

D

D

L

I

T

V

T

T

M

V

A

A

K
|
K

S

A

L

L

A

G

G

G

F

I

P

P

L

I

A

G

A

A

V

T

T

I

G

F

R

R

A

A

E

D

I

-

M

L

D

D

A

F

P

G

G

V

P

S

G

G

G

V

L

H

G

S

G

N

T

T

Y

F

G

G

N

N

P

T

L

V

G

A

I

A

A

A

H

L

A

A

V

V

L

I

D

E

V

E

I

L

A

-

E

Q

E

N

N

G

L

L

C

I

E

E

R

N

A

A

N

Q

Q

K

L

L

G

E

N

P

R

L

L

F

K

R

Q

E

R

R

L

L

A

E

I

M

R

K

E

E

K

K

A

Y

P

E

E

I

I

I

V

G

D

D

I

V

R

R

G

G

P

L

G

G

F

L

M

A

N

W

A

G

V

V

E

E

F

F

-

V

N

K

D

D

V

R

K

K

T

T

N

K

V

E

P

Y

S

A

A

T

E

K

F

E

A

R

N

G

K

E

V

I

R

V

L

V

L

E

A

A

L

L

E

K

K

R

G

G

L

L

I

A

L

L

T

G

C

C

G

G

V

-

H

-

G

K

N

S

V

A

I

I

R

R

F

L

L

I

A

P

P

P

I

L

T

I

I

I

Q

S

D

E

V

E

F

A

A

K

E

M

A

G

L

L

D

D

I

I

L

F

E

E

S

E

S

A

I

I

binding L-ornithine: F149 (= F135), R152 (= R138), E234 (= E208), K291 (= K265)
binding pyridoxal-5'-phosphate: G122 (= G108), T123 (≠ A109), F149 (= F135), H150 (= H136), E229 (= E203), D262 (= D236), V264 (= V238), Q265 (= Q239), K291 (= K265)

7vnoA Structure of aminotransferase (see paper)
40% identity, 93% coverage: 25:417/422 of query aligns to 39:440/452 of 7vnoA

query
sites
7vnoA

E

E

R

R

A

A

E

E

N

G

A

V

E

Y

I

W

W

I

D

D

K

V

E

D

G

G

N

N

R

V

Y

L

I

L

D

D

F

F

A

S

S

S

G

G

I

I

A

G

V

V

V

M

N

N

T

V

G

G

H

L

R

R

H

N

P

P

K

K

V

V

I

I

A

E

A

A

V

I

K

K

A

K

Q

Q

L

L

D

D

R

L

F

V

T

L

H

H

T

A

-

A

-

G

C

T

H

D

Q

Y

V

Y

V

N

P

P

Y

Y

E

Q

N

-

Y

-

V

V

H

E

L

L

A

A

E

K

R

K

L

L

N

V

A

E

I

I

V

A

P

P

G

G

D

D

F

I

A

E

K

R

K

K

T

V

I

F

F

L

V

S

T

N

T

S

G
|
G

A
x
T

E

E

A

A

V
x
N

E

E

N

A

A

A

V

L

K

K

I

I

A

A

R

K

A

W

A

S

T

T

G

N

R

R

Q

K

A

M

I

F

V

I

A

A

F

F

G

I

G

G

G

A

F
|
F

H
|
H

G

G

R

R

T

T

F

H

M

G

G

T

M

M

A

S

L

L

T

T

-

A

G

S

K

K

V

P

V

V

P

Q

Y

R

K

S

V

R

G

M

F

F

G

P

A

T

M

M

P

P

A

G

D

-

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

V

N

E

P

L

Y

H

R

-

N

-

P

-

W

G

G

V

I

S

D

V

G

E

Y

Q

E

S

N

L

P

A

D

A

E

L

L

-

I

-

N

-

R

-

V

-

I

-

D

-

Y

-

I

-

E

K

E

K

Y

L

L

F

F

A

E

A

H

D

Y

V

V

D

P

P

A

N

E

R

E

V

V

A

A

A

G

I

I

I

F

E
|
E

P

P

V

I

Q

Q

G

G

E

E

G

G

F

Y

Y

V

P

V

V

P

P

P

T

K

A

N

F

F

M

F

K

K

A

E

L

L

R

K

E

K

I

L

C

A

D

D

Q

K

N

H

G

G

I

I

L

L

I

I

A

D

D
|
D

E

E

V
|
V

Q
|
Q

T

M

G

G

F

M

A

G

R

R

T

T

G

G

K

R

L

M

L

W

A

A

M

I

E

E

H

H

H

F

G

D

V

I

A

V

P

P

D

D

L

I

T

V

T

T

M

V

A

A

K
|
K

S

A

L

L

A

G

G

G

F

I

P

P

L

I

A

G

A

A

V

T

T

I

G

F

R

R

A

A

E

D

I

-

M

L

D

D

A

F

P

G

G

V

P

S

G

G

G

V

L

H

G

S

G

N

T

T

Y

F

G

G

N

N

P

T

L

V

G

A

I

A

A

A

H

L

A

A

V

V

L

I

D

E

V

E

I

L

A

-

E

Q

E

N

N

G

L

L

C

I

E

E

R

N

A

A

N

Q

Q

K

L

L

G

E

N

P

R

L

L

F

K

R

Q

E

R

R

L

L

A

E

I

M

R

K

E

E

K

K

A

Y

P

E

E

I

I

I

V

G

D

D

I

V

R

R

G

G

P

L

G

G

F

L

M

A

N

W

A

G

V

V

E

E

F

F

-

V

N

K

D

D

V

R

K

K

T

T

N

K

V

E

P

Y

S

A

A

T

E

K

F

E

A

R

N

G

K

E

V

I

R

V

L

V

L

E

A

A

L

L

E

K

K

R

G

G

L

L

I

A

L

L

T

G

C

C

G

G

V

-

H

-

G

K

N

S

V

A

I

I

R

R

F

L

L

I

A

P

P

P

I

L

T

I

I

I

Q

S

D

E

V

E

F

A

A

K

E

M

A

G

L

L

D

D

I

I

L

F

E

E

S

E

S

A

I

I

binding pyridoxal-5'-phosphate: G122 (= G108), T123 (≠ A109), N126 (≠ V112), F149 (= F135), H150 (= H136), E229 (= E203), D262 (= D236), V264 (= V238), Q265 (= Q239), K291 (= K265)

5wyaA Structure of amino acid racemase, 2.65 a (see paper)
33% identity, 99% coverage: 2:417/422 of query aligns to 6:429/439 of 5wyaA

query
sites
5wyaA

T

S

S

K

L

L

T

I

D

D

R

E

K

E

N

N

A

K

A

Y

I

Y

S

A

R

R

G

S

V
x
A

G

R

M

I

T

N

T

Y

-

Y

Q

N

I

L

Y

V

A

I

E

D

R

H

A

A

E

H

N

G

A

A

E

T

I

L

W

V

D

D

K

V

E

D

G

G

N

N

R

K

Y

Y

I

I

D

D

F

L

A

L

S

A

G

S

I
x
A

A

S

V

A

V

I

N

N

T

V

G

G

H

H

R

T

H

H

P

E

K

K

V

V

I

V

A

K

A

A

V

I

K

A

A

D

Q

Q

L

A

D

Q

R

K

F

L

T

I

H

H

T

Y

C

T

H

P

Q

A

V
x
Y

V

F

P

H

Y

H

E

V

N

P

Y

G

V

M

H

E

L

L

A

S

E

E

R

K

L

L

N

A

A

K

I

I

V

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

I

S

F

F

V

G

T

N

T
x
S

G
|
G

A
x
S

E

D

A

A

V

N

E

D

N

A

A

I

V

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

Q

Q

A

Y

I

I

V

V

A

S

F

Y

G

M

G

G

G

S

F
x
Y

H
|
H

G

G

R

S

T

T

F

Y

M

G

G

S

M

Q

A

T

L

L

T

S

G

G

K

S

V

S

V

L

P

N

Y

M

K

T

V

R

G

K

F

I

G

G

A

P

M

M

P

L

A

P

D

S

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

V

D

E

S

L

Y

-

R

-

T

-

Y

-

P

-

G

-

E

-

T

-

E

H

H

G

D

V

V

S

S

V

L

E

-

Q

R

S

Y

L

F

A

N

A

E

L

F

K

K

L

P

F

F

A

E

A

S

D

F

V

L

D

P

P

A

N

D

R

E

V

T

A

A

A

C

I

V

I

L

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E

D

G

G

F

I

Y

I

P

K

V

A

P

P

T

E

A

E

F

Y

M

M

K

Q

A

L

L

V

R

Y

E

K

I

F

C

C

D

H

Q

E

N

H

G

G

I

I

L

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

T

Q

G

G

F

L

A

G

R

R

T

T

G

G

K

K

L

M

L

W

A

A

M

I

E

Q

H

Q

-

F

H

K

G

D

V

I

A

E

P

P

D

D

L

L

T

M

T

S

M

V

A

G

K
|
K

S

S

L

L

A

A

G

S

G

G

F

M

P

P

L

L

A

S

A

A

V

V

T

I

G

G

R

K

A

K

E

E

I

V

M

M

D

Q

A

S

P

L

G

D

P

A

G

P

G

A

L

H

G

L

G
x
F

T
|
T

Y

T

G

A

G

G

N

N

P

P

L

V

G

C

I

S

A

A

A

S

H

L

A

A

V

T

L

L

D

D

V

V

I

I

A

E

E

Y

E

E

N

G

L

L

C

V

E

E

R

K

A

S

N

A

Q

T

L

D

G

G

N

A

R

Y

L

A

K

K

Q

Q

R

R

L

F

A

L

A

E

I

M

R

Q

E

Q

K

R

A

H

P

P

E

M

I

I

V

G

D

D

I

V

R

R

G

M

P

W

G

G

F

L

M

N

N

G

A

G

V

I

E

E

F

L

-

V

N

K

D

D

V

P

K

K

T

T

N

K

V

E

P

P

S

D

A

S

E

D

F

A

A

A

N

T

K

K

V

V

R

I

L

Y

A

A

L

F

E

A

K

H

G

G

L

V

I

V

L

I

T

T

C

L

G

A

V

-

H

-

G

G

N

N

V

I

I

L

R
|
R

F

F

L

Q

A

P

P

P

I

L

T

V

I

I

Q

P

D

R

D

E

V

Q

F

L

A

D

E

Q

A

A

L

L

D

Q

I

V

L

L

E

D

S

D

S

A

I

F

active site: A20 (≠ V16), Y140 (≠ F135), E215 (= E203), D248 (= D236), N251 (≠ Q239), K278 (= K265), T307 (= T294), R406 (= R394)
binding (2S,3S)-3-methyl-2-[[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylamino]pentanoic acid: A52 (≠ I47), Y82 (≠ V77), S112 (≠ T107), G113 (= G108), S114 (≠ A109), Y140 (≠ F135), H141 (= H136), E215 (= E203), D248 (= D236), V250 (= V238), N251 (≠ Q239), K278 (= K265), F306 (≠ G293), T307 (= T294), R406 (= R394)

4ysnC Structure of aminoacid racemase in complex with plp (see paper)
33% identity, 99% coverage: 2:417/422 of query aligns to 15:438/448 of 4ysnC

query
sites
4ysnC

T

S

S

K

L

L

T

I

D

D

R

E

K

E

N

N

A

K

A

Y

I

Y

S

A

R

R

G

S

V
x
A

G

R

M

I

T

N

T

Y

-

Y

Q

N

I

L

Y

V

A

I

E

D

R

H

A

A

E

H

N

G

A

A

E

T

I

L

W

V

D

D

K

V

E

D

G

G

N

N

R

K

Y

Y

I

I

D

D

F

L

A

L

S

A

G

S

I

A

A

S

V

A

V

I

N

N

T

V

G

G

H

H

R

T

H

H

P

E

K

K

V

V

I

V

A

K

A

A

V

I

K

A

A

D

Q

Q

L

A

D

Q

R

K

F

L

T

I

H

H

T

Y

C

T

H

P

Q

A

V

Y

V

F

P

H

Y

H

E

V

N

P

Y

G

V

M

H

E

L

L

A

S

E

E

R

K

L

L

N

A

A

K

I

I

V

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

I

S

F

F

V

G

T

N

T
x
S

G
|
G

A
x
S

E

D

A

A

V

N

E

D

N

A

A

I

V

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

Q

Q

A

Y

I

I

V

V

A

S

F

Y

G

M

G

G

G

S

F
x
Y

H
|
H

G

G

R

S

T

T

F

Y

M

G

G

S

M

Q

A

T

L

L

T

S

G

G

K

S

V

S

V

L

P

N

Y

M

K

T

V

R

G

K

F

I

G

G

A

P

M

M

P

L

A

P

D

S

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

V

D

E

S

L

Y

-

R

-

T

-

Y

-

P

-

G

-

E

-

T

-

E

H

H

G

D

V

V

S

S

V

L

E

-

Q

R

S

Y

L

F

A

N

A

E

L

F

K

K

L

P

F

F

A

E

A

S

D

F

V

L

D

P

P

A

N

D

R

E

V

T

A

A

A

C

I

V

I

L

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E

D

G

G

F

I

Y

I

P

K

V

A

P

P

T

E

A

E

F

Y

M

M

K

Q

A

L

L

V

R

Y

E

K

I

F

C

C

D

H

Q

E

N

H

G

G

I

I

L

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

T

Q

G

G

F

L

A

G

R

R

T

T

G

G

K

K

L

M

L

W

A

A

M

I

E

Q

H

Q

-

F

H

K

G

D

V

I

A

E

P

P

D

D

L

L

T

M

T

S

M

V

A

G

K
|
K

S

S

L

L

A

A

G

S

G

G

F

M

P

P

L

L

A

S

A

A

V

V

T

I

G

G

R

K

A

K

E

E

I

V

M

M

D

Q

A

S

P

L

G

D

P

A

G

P

G

A

L

H

G

L

G
x
F

T
|
T

Y

T

G

A

G

G

N

N

P

P

L

V

G

C

I

S

A

A

A

S

H

L

A

A

V

T

L

L

D

D

V

V

I

I

A

E

E

Y

E

E

N

G

L

L

C

V

E

E

R

K

A

S

N

A

Q

T

L

D

G

G

N

A

R

Y

L

A

K

K

Q

Q

R

R

L

F

A

L

A

E

I

M

R

Q

E

Q

K

R

A

H

P

P

E

M

I

I

V

G

D

D

I

V

R

R

G

M

P

W

G

G

F

L

M

N

N

G

A

G

V

I

E

E

F

L

-

V

N

K

D

D

V

P

K

K

T

T

N

K

V

E

P

P

S

D

A

S

E

D

F

A

A

A

N

T

K

K

V

V

R

I

L

Y

A

A

L

F

E

A

K

H

G

G

L

V

I

V

L

I

T

T

C

L

G

A

V

-

H

-

G

G

N

N

V

I

I

L

R
|
R

F

F

L

Q

A

P

P

P

I

L

T

V

I

I

Q

P

D

R

D

E

V

Q

F

L

A

D

E

Q

A

A

L

L

D

Q

I

V

L

L

E

D

S

D

S

A

I

F

active site: A29 (≠ V16), Y149 (≠ F135), E224 (= E203), D257 (= D236), N260 (≠ Q239), K287 (= K265), T316 (= T294), R415 (= R394)
binding pyridoxal-5'-phosphate: S121 (≠ T107), G122 (= G108), S123 (≠ A109), Y149 (≠ F135), H150 (= H136), E224 (= E203), D257 (= D236), V259 (= V238), K287 (= K265), F315 (≠ G293), T316 (= T294)

5wyfA Structure of amino acid racemase, 2.12 a (see paper)
33% identity, 99% coverage: 2:417/422 of query aligns to 8:431/446 of 5wyfA

query
sites
5wyfA

T

S

S

K

L

L

T

I

D

D

R

E

K

E

N

N

A

K

A

Y

I

Y

S

A

R

R

G

S

V
x
A

G

R

M

I

T

N

T

Y

-

Y

Q

N

I

L

Y

V

A

I

E

D

R

H

A

A

E

H

N

G

A

A

E

T

I

L

W

V

D

D

K

V

E

D

G

G

N

N

R

K

Y

Y

I

I

D

D

F

L

A

L

S

A

G

S

I
x
A

A

S

V

A

V

I

N

N

T

V

G

G

H

H

R

T

H

H

P

E

K

K

V

V

I

V

A

K

A

A

V

I

K

A

A

D

Q

Q

L

A

D

Q

R

K

F

L

T

I

H

H

T

Y

C

T

H

P

Q

A

V
x
Y

V

F

P

H

Y

H

E

V

N

P

Y

G

V

M

H

E

L

L

A

S

E

E

R

K

L

L

N

A

A

K

I

I

V

A

P

P

G

G

D

N

F

S

A

P

K

K

K

M

T

V

I

S

F

F

V

G

T

N

T

S

G
|
G

A
x
S

E

D

A

A

V

N

E

D

N

A

A

I

V

I

K

K

I

F

A

A

R

R

A

A

A

Y

T

T

G

G

R

R

Q

Q

A

Y

I

I

V

V

A

S

F

Y

G

M

G

G

G

S

F
x
Y

H
|
H

G

G

R

S

T

T

F

Y

M

G

G

S

M

Q

A

T

L

L

T

S

G

G

K

S

V

S

V

L

P

N

Y

M

K

T

V

R

G

K

F

I

G

G

A

P

M

M

P

L

A

P

D

S

V

V

F

V

H

H

A

V

P

P

F

Y

P

P

V

D

E

S

L

Y

-

R

-

T

-

Y

-

P

-

G

-

E

-

T

-

E

H

H

G

D

V

V

S

S

V

L

E

-

Q

R

S

Y

L

F

A

N

A

E

L

F

K

K

L

P

F

F

A

E

A

S

D

F

V

L

D

P

P

A

N

D

R

E

V

T

A

A

A

C

I

V

I

L

I

I

E
|
E

P

P

V

I

Q

Q

G

G

E
x
D

G

G

F

I

Y

I

P

K

V

A

P

P

T

E

A

E

F

Y

M

M

K

Q

A

L

L

V

R

Y

E

K

I

F

C

C

D

H

Q

E

N

H

G

G

I

I

L

L

L

F

I

A

A

I

D
|
D

E

E

V
|
V

Q
x
N

T

Q

G

G

F

L

A

G

R

R

T

T

G

G

K

K

L

M

L

W

A

A

M

I

E

Q

H

Q

-

F

H

K

G

D

V

I

A

E

P

P

D

D

L

L

T

M

T

S

M

V

A

G

K
|
K

S

S

L

L

A

A

G

S

G

G

F

M

P

P

L

L

A

S

A

A

V

V

T

I

G

G

R

K

A

K

E

E

I

V

M

M

D

Q

A

S

P

L

G

D

P

A

G

P

G

A

L

H

G

L

G
x
F

T
|
T

Y

T

G

A

G

G

N

N

P

P

L

V

G

C

I

S

A

A

A

S

H

L

A

A

V

T

L

L

D

D

V

V

I

I

A

E

E

Y

E

E

N

G

L

L

C

V

E

E

R

K

A

S

N

A

Q

T

L

D

G

G

N

A

R

Y

L

A

K

K

Q

Q

R

R

L

F

A

L

A

E

I

M

R

Q

E

Q

K

R

A

H

P

P

E

M

I

I

V

G

D

D

I

V

R

R

G

M

P

W

G

G

F

L

M

N

N

G

A

G

V

I

E

E

F

L

-

V

N

K

D

D

V

P

K

K

T

T

N

K

V

E

P

P

S

D

A

S

E

D

F

A

A

A

N

T

K

K

V

V

R

I

L

Y

A

A

L

F

E

A

K

H

G

G

L

V

I

V

L

I

T

T

C

L

G

A

V

-

H

-

G

G

N

N

V

I

I

L

R
|
R

F

F

L

Q

A

P

P

P

I

L

T

V

I

I

Q

P

D

R

D

E

V

Q

F

L

A

D

E

Q

A

A

L

L

D

Q

I

V

L

L

E

D

S

D

S

A

I

F

active site: A22 (≠ V16), Y142 (≠ F135), E217 (= E203), D250 (= D236), N253 (≠ Q239), K280 (= K265), T309 (= T294), R408 (= R394)
binding n-[o-phosphono-pyridoxyl]-isoleucine: A54 (≠ I47), Y84 (≠ V77), G115 (= G108), S116 (≠ A109), Y142 (≠ F135), H143 (= H136), D222 (≠ E208), D250 (= D236), V252 (= V238), N253 (≠ Q239), K280 (= K265), F308 (≠ G293), T309 (= T294), R408 (= R394)

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
39% identity, 90% coverage: 26:404/422 of query aligns to 33:381/395 of Q5SHH5

query
sites
Q5SHH5

R

R

A

G

E

Q

N

G

A

A

E

R

I

V

W

W

D

D

K

A

E

E

G

G

N

N

R

E

Y

Y

I

I

D

D

F

C

A

V

S

G

G

G

I

Y

A

G

V

V

V

A

N

N

T

L

G

G

H

H

R

G

H

N

P

P

K

E

V

V

I

V

A

E

A

A

V

V

K

K

A

R

Q

Q

L

A

D

E

R

T

F

L

T

M

H

-

T

A

C

M

H

P

Q

Q

V

T

V

L

P

P

Y

T

E

P

N

M

Y

R

V

G

H

E

L

F

A

Y

E

R

R

T

L

L

N

T

A

A

I

I

V

L

P

P

G

P

D

E

F

L

A

-

K

N

K

R

T

V

I

F

F

P

V

V

T

N

T

S

G
|
G

A
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

F

A

A

R

R

A

A

A

H

T

T

G

G

R

R

Q

K

A

K

I

F

V

V

A

A

F

A

G

M

G

R

G

G

F

F

H

S

G

G

R

R

T

T

F

M

M

G

G

S

M

L

A

S

L

V

T

T

G

W

K

E

V

P

V

-

P

K

Y

Y

K

R

V

E

G

P

F

F

G

L

A

P

M

L

P

V

A

E

D

P

V

V

F

E

H

F

A

I

P

P

F

Y

P

-

V

-

E

-

L

-

H

-

G

-

V

-

S

-

V

-

E

-

Q

N

S

D

L

V

A

E

A

A

L

L

K

K

K

R

L

-

F

-

A

A

A

V

D

D

V

E

D

E

P

-

N

-

R

-

V

T

A

A

I

V

I

I

I

L

E

E

P

P

V

V

Q

Q

G

G

E

E

G

G

F

V

Y

R

P

P

V

A

P

T

T

P

A

E

F

F

M

L

K

R

A

A

L

A

R

R

E

E

I

I

C

T

D

Q

Q

E

N

K

G

G

I

A

L

L

I

I

A

L

D

D

E

E

V

I

Q

Q

T

T

G

G

F

M

A

G

R

R

T

T

G

G

K

K

L

R

L

F

A

A

M

F

E

E

H

H

H

F

G

G

V

I

A

V

P

P

D

D

L

I

T

L

T

T

M

L

A

A

K
|
K

S

A

L

L

A

G

G

G

F

V

P

P

L

L

A

G

A

A

V

A

T

V

G

M

R

R

A

E

E

E

I

V

M

A

D

R

A

S

P

M

G

P

P

K

G

G

L

H

G

G

G

T

T
|
T

Y

F

G

G

N

N

P

P

L

L

G

A

I

M

A

A

A

G

H

V

A

A

V

A

L

I

D

R

V

Y

I

L

A

E

E

R

E

T

N

R

L

L

C

W

E

E

R

R

A

A

N

A

Q

E

L

L

G

G

N

P

R

W

L

F

K

M

Q

E

R

K

L

L

A

R

A

A

I

I

R

-

E

-

K

P

A

S

P

P

E

K

I

I

V

R

D

E

I

V

R

R

G

G

P

M

G

G

F

L

M

M

N

V

A

G

V

L

E

E

F

L

N

K

D

E

V

-

K

K

T

A

N

A

V

P

P

Y

S

I

A

A

E

R

F

L

A

E

N

K

K

E

V

H

R

R

L

V

L

L

A

A

L

L

E

Q

K

A

G

G

L

-

I

-

L

-

T

-

C

-

G

-

V

-

H

-

G

P

N

T

V

V

I

I

R

R

F

F

L

L

A

P

P

P

I

L

T

V

I

I

Q

E

D

K

D

E

GT 113:114 (≠ GA 108:109) binding pyridoxal 5'-phosphate
K254 (= K265) modified: N6-(pyridoxal phosphate)lysine
T283 (= T294) binding pyridoxal 5'-phosphate

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 90% coverage: 26:404/422 of query aligns to 25:373/387 of 1wkhA

query
sites
1wkhA

R

R

A

G

E

Q

N

G

A

A

E

R

I

V

W

W

D

D

K

A

E

E

G

G

N

N

R

E

Y

Y

I

I

D

D

F

C

A

V

S

G

G

G

I
x
Y

A

G

V

V

V

A

N

N

T

L

G

G

H

H

R

G

H

N

P

P

K

E

V

V

I

V

A

E

A

A

V

V

K

K

A

R

Q

Q

L

A

D

E

R

T

F

L

T

M

H

-

T

A

C

M

H

P

Q

Q

V

T

V

L

P

P

Y

T

E

P

N

M

Y

R

V

G

H

E

L

F

A

Y

E

R

R

T

L

L

N

T

A

A

I

I

V

L

P

P

G

P

D

E

F

L

A

-

K

N

K

R

T

V

I

F

F

P

V

V

T

N

T
x
S

G
|
G

A
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

F

A

A

R

R

A

A

A

H

T

T

G

G

R

R

Q

K

A

K

I

F

V

V

A

A

F

A

G

M

G

R

G

G

F
|
F

H
x
S

G

G

R

R

T

T

F

M

M

G

G

S

M

L

A

S

L

V

T

T

G

W

K

E

V

P

V

-

P

K

Y

Y

K

R

V

E

G

P

F

F

G

L

A

P

M

L

P

V

A

E

D

P

V

V

F

E

H

F

A

I

P

P

F

Y

P

-

V

-

E

-

L

-

H

-

G

-

V

-

S

-

V

-

E

-

Q

N

S

D

L

V

A

E

A

A

L

L

K

K

K

R

L

-

F

-

A

A

A

V

D

D

V

E

D

E

P

-

N

-

R

-

V

T

A

A

I

V

I

I

I

L

E
|
E

P

P

V

V

Q

Q

G

G

E
|
E

G

G

F

V

Y

R

P

P

V

A

P

T

T

P

A

E

F

F

M

L

K

R

A

A

L

A

R

R

E

E

I

I

C

T

D

Q

Q

E

N

K

G

G

I

A

L

L

I

I

A

L

D
|
D

E

E

V
x
I

Q
|
Q

T

T

G

G

F

M

A

G

R

R

T

T

G

G

K

K

L

R

L

F

A

A

M

F

E

E

H

H

H

F

G

G

V

I

A

V

P

P

D

D

L

I

T

L

T

T

M

L

A

A

K
|
K

S

A

L

L

A

G

G

G

F

V

P

P

L

L

A

G

A

V

V

A

T

V

G

M

R

R

A

E

E

E

I

V

M

A

D

R

A

S

P

M

G

P

P

K

G

G

L

H

G

G

G

T

T
|
T

Y

F

G

G

N

N

P

P

L

L

G

A

I

M

A

A

A

G

H

V

A

A

V

A

L

I

D

R

V

Y

I

L

A

E

E

R

E

T

N

R

L

L

C

W

E

E

R

R

A

A

N

A

Q

E

L

L

G

G

N

P

R

W

L

F

K

M

Q

E

R

K

L

L

A

R

A

A

I

I

R

-

E

-

K

P

A

S

P

P

E

K

I

I

V

R

D

E

I

V

R

R

G

G

P

M

G

G

F

L

M

M

N

V

A

G

V

L

E

E

F

L

N

K

D

E

V

-

K

K

T

A

N

A

V

P

P

Y

S

I

A

A

E

R

F

L

A

E

N

K

K

E

V

H

R

R

L

V

L

L

A

A

L

L

E

Q

K

A

G

G

L

-

I

-

L

-

T

-

C

-

G

-

V

-

H

-

G

P

N

T

V

V

I

I

R
|
R

F

F

L

L

A

P

P

P

I

L

T

V

I

I

Q

E

D

K

D

E

active site: F132 (= F135), E184 (= E203), D217 (= D236), Q220 (= Q239), K246 (= K265), T275 (= T294), R363 (= R394)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ I47), S104 (≠ T107), G105 (= G108), T106 (≠ A109), F132 (= F135), S133 (≠ H136), E184 (= E203), E189 (= E208), D217 (= D236), I219 (≠ V238), K246 (= K265), R363 (= R394)

Sites not aligning to the query:

active site: 13

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 90% coverage: 26:404/422 of query aligns to 25:373/387 of 1wkgA

query
sites
1wkgA

R

R

A

G

E

Q

N

G

A

A

E

R

I

V

W

W

D

D

K

A

E

E

G

G

N

N

R

E

Y

Y

I

I

D

D

F

C

A

V

S

G

G

G

I
x
Y

A

G

V

V

V

A

N

N

T

L

G

G

H

H

R

G

H

N

P

P

K

E

V

V

I

V

A

E

A

A

V

V

K

K

A

R

Q

Q

L

A

D

E

R

T

F

L

T

M

H

-

T

A

C

M

H

P

Q

Q

V

T

V

L

P

P

Y

T

E

P

N

M

Y

R

V

G

H

E

L

F

A

Y

E

R

R

T

L

L

N

T

A

A

I

I

V

L

P

P

G

P

D

E

F

L

A

-

K

N

K

R

T

V

I

F

F

P

V

V

T

N

T

S

G
|
G

A
x
T

E

E

A

A

V

N

E

E

N

A

A

A

V

L

K

K

I

F

A

A

R

R

A

A

A

H

T

T

G

G

R

R

Q

K

A

K

I

F

V

V

A

A

F

A

G

M

G

R

G

G

F
|
F

H
x
S

G

G

R
|
R

T

T

F

M

M

G

G

S

M

L

A

S

L

V

T

T

G

W

K

E

V

P

V

-

P

K

Y

Y

K

R

V

E

G

P

F

F

G

L

A

P

M

L

P

V

A

E

D

P

V

V

F

E

H

F

A

I

P

P

F

Y

P

-

V

-

E

-

L

-

H

-

G

-

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S

-

V

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E

-

Q

N

S

D

L

V

A

E

A

A

L

L

K

K

K

R

L

-

F

-

A

A

A

V

D

D

V

E

D

E

P

-

N

-

R

-

V

T

A

A

I

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I

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E
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E

P

P

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V

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Q

G

G

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E

G

G

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P

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T

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F

M

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A

A

L

A

R

R

E

E

I

I

C

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D

Q

Q

E

N

K

G

G

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L

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I

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D
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D

E

E

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x
I

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Q

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T

G

G

F

M

A

G

R

R

T

T

G

G

K

K

L

R

L

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A

A

M

F

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E

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G

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I

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D

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S

A

L

L

A

G

G

G

F

V

P

P

L

L

A

G

A

V

V

A

T

V

G

M

R

R

A

E

E

E

I

V

M

A

D

R

A

S

P

M

G

P

P

K

G

G

L

H

G
|
G

G
x
T

T
|
T

Y

F

G

G

N

N

P

P

L

L

G

A

I

M

A

A

A

G

H

V

A

A

V

A

L

I

D

R

V

Y

I

L

A

E

E

R

E

T

N

R

L

L

C

W

E

E

R

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A

A

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A

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E

L

L

G

G

N

P

R

W

L

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M

Q

E

R

K

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L

A

R

A

A

I

I

R

-

E

-

K

P

A

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K

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D

E

I

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R

G

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-

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A

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R

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V

L

L

A

A

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A

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G

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I

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L

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R

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active site: F132 (= F135), E184 (= E203), D217 (= D236), Q220 (= Q239), K246 (= K265), T275 (= T294), R363 (= R394)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y46 (≠ I47), G105 (= G108), T106 (≠ A109), F132 (= F135), S133 (≠ H136), R135 (= R138), E184 (= E203), D217 (= D236), I219 (≠ V238), Q220 (= Q239), K246 (= K265), G273 (= G292), T274 (≠ G293), T275 (= T294)

Sites not aligning to the query:

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
38% identity, 90% coverage: 26:404/422 of query aligns to 25:373/387 of 1vefA

query
sites
1vefA

R

R

A

G

E

Q

N

G

A

A

E

R

I

V

W

W

D

D

K

A

E

E

G

G

N

N

R

E

Y

Y

I

I

D

D

F

C

A

V

S

G

G

G

I

Y

A

G

V

V

V

A

N

N

T

L

G

G

H

H

R

G

H

N

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P

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E

V

V

I

V

A

E

A

A

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V

K

K

A

R

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Q

L

A

D

E

R

T

F

L

T

M

H

-

T

A

C

M

H

P

Q

Q

V

T

V

L

P

P

Y

T

E

P

N

M

Y

R

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H

E

L

F

A

Y

E

R

R

T

L

L

N

T

A

A

I

I

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L

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P

G

P

D

E

F

L

A

-

K

N

K

R

T

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I

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F

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V

V

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N

T

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G
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G

A
x
T

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E

A

A

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N

E

E

N

A

A

A

V

L

K

K

I

F

A

A

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R

A

A

A

H

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T

G

G

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R

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K

A

K

I

F

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V

A

A

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A

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G

F
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F

H
x
S

G

G

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R

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T

F

M

M

G

G

S

M

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A

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L

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T

G

W

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Y

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E

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F

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A

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L

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A

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D

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H

F

A

I

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F

Y

P

-

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-

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-

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A

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K

R

L

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F

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A

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E

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G

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E

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F

M

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A

A

L

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G

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L

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D

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x
I

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Q

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T

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G

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A

G

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R

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T

G

G

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K

L

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L

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A

M

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E

H

H

H

F

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G

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I

A

V

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P

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D

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K

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A

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L

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G

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M

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G

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T

Y

F

G

G

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N

P

P

L

L

G

A

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A

A

A

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A

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Y

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E

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R

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E

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G

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M

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K

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L

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R

A

A

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I

R

-

E

-

K

P

A

S

P

P

E

K

I

I

V

R

D

E

I

V

R

R

G

G

P

M

G

G

F

L

M

M

N

V

A

G

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E

F

L

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K

D

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K

K

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K

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A

A

L

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E

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G

L

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L

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active site: F132 (= F135), D217 (= D236), K246 (= K265), T275 (= T294), R363 (= R394)
binding pyridoxal-5'-phosphate: G105 (= G108), T106 (≠ A109), F132 (= F135), S133 (≠ H136), E184 (= E203), D217 (= D236), I219 (≠ V238), K246 (= K265)

Sites not aligning to the query:

active site: 13

Query Sequence

>WP_012061607.1 NCBI__GCF_000017145.1:WP_012061607.1
MTSLTDRKNAAISRGVGMTTQIYAERAENAEIWDKEGNRYIDFASGIAVVNTGHRHPKVI
AAVKAQLDRFTHTCHQVVPYENYVHLAERLNAIVPGDFAKKTIFVTTGAEAVENAVKIAR
AATGRQAIVAFGGGFHGRTFMGMALTGKVVPYKVGFGAMPADVFHAPFPVELHGVSVEQS
LAALKKLFAADVDPNRVAAIIIEPVQGEGGFYPVPTAFMKALREICDQNGILLIADEVQT
GFARTGKLLAMEHHGVAPDLTTMAKSLAGGFPLAAVTGRAEIMDAPGPGGLGGTYGGNPL
GIAAAHAVLDVIAEENLCERANQLGNRLKQRLAAIREKAPEIVDIRGPGFMNAVEFNDVK
TNVPSAEFANKVRLLALEKGLILLTCGVHGNVIRFLAPITIQDDVFAEALDILESSILEA
RG

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory