SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012061729.1 NCBI__GCF_000017145.1:WP_012061729.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

G3XD01 UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronate N-acetyltransferase; UDP-D-GlcNAc3NA N-acetyltransferase; UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucuronic acid 3-N-acetyltransferase; EC 2.3.1.201 from Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) (see paper)
41% identity, 80% coverage: 24:156/166 of query aligns to 32:157/191 of G3XD01

query
sites
G3XD01

G

G

C

A

T

R

I

I

G

G

S

A

G

G

T

V

R

S

I

L

G

G

T

Q

F

N

V

V

E

F

I

V

Q

G

K

N

N

K

T

V

I

I

G

G

R

D

N

R

C

C

K

K

I

I

S

Q

S

N

H

N

S

V

F

S

L

V

C

Y

E

D

G

N

V

V

T

T

L

L

E

E

D

E

G

G

V

V

F

F

I

C

G

G

H

P

G

S

V

M

M

V

F

F

T

T

N

N

D

V

L

Y

Y

N

P

P

R

R

A

S

I

L

-

I

-

E

N

R

A

K

D

D

G

Q

G

Y

R

R

Q

N

S

-

E

-

G

-

D

-

W

-

T

-

V

-

P

-

T

T

R

L

V

V

K

K

Q

K

R

G

V

A

S

T

I

L

G

G

S

A

N

N

A

C

T

T

I

I

L

V

A

C

G

G

I

V

T

T

I

I

G

G

E

E

S

Y

A

A

Q

F

V

V

G

G

A

A

G

G

A

A

V

V

V

I

T

N

R
x
K

D

N

V

V

P

P

A

S

Y

Y

T

A

I

L

V

M

A

V

G

G

V

V

P

P

A

A

R

R

M

Q

I

I

G

G

H

W

T

M

K

S

D

E

K136 (≠ R135) mutation to A: Reduction in catalytic activity.; mutation to R: No effect on catalytic activity.

7s42A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- galactose (see paper)
42% identity, 72% coverage: 19:137/166 of query aligns to 7:135/145 of 7s42A

query
sites
7s42A

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

T

N

I

I

G

G

S

S

G

G

T

T

R

R

I

I

G

W

T

Q

F

F

V

C

E

V

I

V

Q

L

K

P

N

S

T

A

T

I

I

I

G

G

R

E

N

N

C

C

K

N

I

I

S

C

S

S

H

H

S

C

F
|
F

L

I

-
x
E

-

N

-

D

-

V

-

K

-

I

-

G

-

N

-

V

-

T

-

I

-

K

C

C

-

G

-

V

-

Q

-

I

-
x
W

E

D

G

G

V

I

T

E

L

I

E

E

D

D

G

D

V

V

F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N
|
N

D
|
D

L
x
K

Y
|
Y

P
|
P

R

R

A

S

I

-

N

-

A

-

D

-

G

-

R

K

Q

Q

S
x
Y

E

P

G

K

D

E

W

F

T

S

V

-

P

K

T

T

R

I

V

I

K

K

Q

K

R

G

V

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
|
L

A

P

G

G

I

I

T

T

I

I

G

G

E

E

S

N

A

A

Q

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding [(2~{R},3~{R},4~{S},5~{R},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate: F44 (= F56), E46 (vs. gap), W64 (vs. gap), N83 (= N77), D84 (= D78), Y92 (≠ S92)
binding coenzyme a: C82 (≠ T76), D84 (= D78), K85 (≠ L79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (≠ R135)

7s41A Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-acetamido-3,6-dideoxy-d- glucose (see paper)
42% identity, 72% coverage: 19:137/166 of query aligns to 7:135/145 of 7s41A

query
sites
7s41A

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

T

N

I

I

G

G

S

S

G

G

T

T

R

R

I

I

G

W

T

Q

F

F

V

C

E

V

I

V

Q

L

K

P

N

S

T

A

T

I

I

I

G

G

R

E

N

N

C

C

K

N

I

I

S

C

S

S

H

H

S

C

F
|
F

L

I

-
x
E

-

N

-

D

-

V

-

K

-

I

-

G

-

N

-

V

-

T

-

I

-

K

C

C

-

G

-

V

-

Q

-

I

-
x
W

E

D

G

G

V

I

T

E

L

I

E

E

D

D

G

D

V

V

F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N
|
N

D
|
D

L
x
K

Y
|
Y

P
|
P

R

R

A

S

I

-

N

-

A

-

D

-

G

-

R

K

Q

Q

S
x
Y

E

P

G

K

D

E

W

F

T

S

V

-

P

K

T

T

R

I

V

I

K

K

Q

K

R

G

V

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
|
L

A

P

G

G

I

I

T

T

I

I

G

G

E

E

S

N

A

A

Q

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding [(2~{R},3~{R},4~{S},5~{S},6~{R})-4-acetamido-6-methyl-3,5-bis(oxidanyl)oxan-2-yl] [[(2~{R},3~{S},5~{R})-5-[5-methyl-2,4-bis(oxidanylidene)pyrimidin-1-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] hydrogen phosphate : F44 (= F56), E46 (vs. gap), W64 (vs. gap), N83 (= N77), D84 (= D78), Y92 (≠ S92)
binding coenzyme a: C82 (≠ T76), D84 (= D78), K85 (≠ L79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (≠ R135)

7s3wA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d- galactose (see paper)
42% identity, 72% coverage: 19:137/166 of query aligns to 7:135/145 of 7s3wA

query
sites
7s3wA

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

T

N

I

I

G

G

S

S

G

G

T

T

R

R

I

I

G

W

T

Q

F

F

V

C

E

V

I

V

Q

L

K

P

N

S

T

A

T

I

I

I

G

G

R

E

N

N

C

C

K

N

I

I

S

C

S

S

H

H

S

C

F
|
F

L

I

-
x
E

-

N

-

D

-

V

-

K

-

I

-

G

-

N

-

V

-

T

-

I

-

K

C

C

-

G

-

V

-

Q

-

I

-

W

E

D

G

G

V

I

T

E

L

I

E

E

D

D

G

D

V

V

F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N

N

D
|
D

L
x
K

Y
|
Y

P
|
P

R

R

A

S

I

-

N

-

A

-

D

-

G

-

R

K

Q

Q

S
x
Y

E

P

G

K

D

E

W

F

T

S

V

-

P

K

T

T

R

I

V

I

K

K

Q

K

R

G

V

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
|
L

A

P

G

G

I

I

T

T

I

I

G

G

E

E

S

N

A

A

Q

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding oxidized coenzyme a: C82 (≠ T76), D84 (= D78), K85 (≠ L79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (≠ R135)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F56), E46 (vs. gap), D84 (= D78), Y92 (≠ S92)

7s3uA Crystal structure of an n-acetyltransferase from helicobacter pullorum in the presence of coenzyme a and dtdp-3-amino-3,6-dideoxy-d-glucose (see paper)
42% identity, 72% coverage: 19:137/166 of query aligns to 7:135/145 of 7s3uA

query
sites
7s3uA

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

T

N

I

I

G

G

S

S

G

G

T

T

R

R

I

I

G

W

T

Q

F

F

V

C

E

V

I

V

Q

L

K

P

N

S

T

A

T

I

I

I

G

G

R

E

N

N

C

C

K

N

I

I

S

C

S

S

H

H

S

C

F
|
F

L

I

-
x
E

-

N

-

D

-

V

-

K

-

I

-

G

-

N

-

V

-

T

-

I

-

K

C

C

-

G

-

V

-

Q

-

I

-

W

E

D

G

G

V

I

T

E

L

I

E

E

D

D

G

D

V

V

F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

T
x
C

N
|
N

D
|
D

L
x
K

Y
|
Y

P
|
P

R

R

A

S

I

-

N

-

A

-

D

-

G

-

R

K

Q

Q

S
x
Y

E

P

G

K

D

E

W

F

T

S

V

-

P

K

T

T

R

I

V

I

K

K

Q

K

R

G

V

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
|
L

A

P

G

G

I

I

T

T

I

I

G

G

E

E

S

N

A

A

Q

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding coenzyme a: C82 (≠ T76), N83 (= N77), D84 (= D78), K85 (≠ L79), Y86 (= Y80), P87 (= P81), L114 (= L116), T132 (= T134), K133 (≠ R135)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: F44 (= F56), E46 (vs. gap), D84 (= D78), Y92 (≠ S92)

7s45A Crystal structure of an n-acetyltransferase, c80t mutant, from helicobacter pullorum in the presence of acetyl coenzyme a and dtdp (see paper)
42% identity, 72% coverage: 19:137/166 of query aligns to 7:131/141 of 7s45A

query
sites
7s45A

L

L

V

A

N

D

L

V

Y

Q

G

S

C

K

T

N

I

I

G

G

S

S

G

G

T

T

R

R

I

I

G

W

T

Q

F

F

V

C

E

V

I

V

Q

L

K

P

N

S

T

A

T

I

I

I

G

G

R

E

N

N

C

C

K

N

I

I

S

C

S

S

H

H

S

C

F
|
F

L

I

-
x
E

-

N

-

D

-

V

-

K

-

I

-

G

-

N

-

V

-

T

-

I

-

K

-

C

-

G

-

V

-

Q

-

I

C
x
W

E

D

G

G

V

I

T

E

L

I

E

E

D

D

G

D

V

V

F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

T
|
T

N
|
N

D
|
D

L
x
K

Y
|
Y

P
|
P

R

R

A

S

I

-

N

-

A

-

D

-

G

-

R

K

Q

Q

S

F

E

S

G

-

D

-

W

-

T

-

V

-

P

K

T

T

R

I

V

I

K

K

Q

K

R

G

V

A

S

S

I

I

G

G

S

A

N

N

A

A

T

T

I

I

L
|
L

A

P

G

G

I

I

T

T

I

I

G

G

E

E

S

N

A

A

Q

M

V

I

G

G

A

A

G

G

A

A

V

I

V

V

T
|
T

R
x
K

D

D

V

V

binding acetyl coenzyme *a: W64 (≠ C58), T82 (= T76), N83 (= N77), D84 (= D78), K85 (≠ L79), Y86 (= Y80), P87 (= P81), L110 (= L116), T128 (= T134), K129 (≠ R135)
binding thymidine-5'-diphosphate: F44 (= F56), E46 (vs. gap)

4mzuB Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
37% identity, 83% coverage: 19:155/166 of query aligns to 5:144/290 of 4mzuB

query
sites
4mzuB

L

L

V

A

N

D

L

V

Y

Q

G

S

C

Q

T

N

I

I

G

G

S

D

G

N

T

T

R

K

I

V

G
x
W

T
x
Q

F

F

V

C

E

V

I

I

Q

L

K

A

N

G

T

A

T

V

I

I

G

G

R

R

N

N

C

C

K

N

I

I

S
x
C

S
x
A

H

N

S

S

F

L

L

I

-

E

-

N

-

D

-

V

-

I

-

G

-

D

-

N

-

V

-

T

-

I

-

K

-

S

-
x
G

-

V

-

Q

-

I

C

W

E

D

G

G

V

I

T

H

L

I

E

Q

D

D

G

D

V

V

F
|
F

I

I

G

G

H

P

G
x
N

V

V

M

T

F

F

T
|
T

N
|
N

D
|
D

L
x
K

Y

Q

P
|
P

R

R

A

S

I

L

N

K

A

-

D

-

G

-

R

-

Q

-

S

-

E

-

G

-

D

-

W

-

T

-

V

-

P

-

T

T

R

I

V

V

K

K

Q

K

R

G

V

A

S

S

I

I

G
|
G

S
x
A

N

N

A

S

T

T

I

I

L

L

A

P

G

G

I

I

T

L

I

I

G

G

E

E

S

N

A

A

Q

M

V

V

G
|
G

A
|
A

G

G

A

A

V

V

V

I

T
|
T

R
x
K

D

N

V

V

P

P

A

D

Y

N

T

A

I

I

V

V

A
x
I

G

G

V

N

P

P

A

G

R
|
R

M

I

I

T

G

G

H

Y

T

V

K

E

binding coenzyme a: F72 (= F68), T80 (= T76), N81 (= N77), D82 (= D78), K83 (≠ L79), P85 (= P81), G99 (= G110), A100 (≠ S111), G117 (= G128), A118 (= A129), T123 (= T134), K124 (≠ R135), I133 (≠ A144), R138 (= R149)
binding magnesium ion: G58 (vs. gap), N76 (≠ G72)
binding thymine: W20 (≠ G34), Q21 (≠ T35), C38 (≠ S52), A39 (≠ S53)

Sites not aligning to the query:

binding coenzyme a: 199
binding thymidine-5'-diphosphate: 171, 176, 189, 191, 202, 203, 270

4mzuF Crystal structure of fdtd, a bifunctional ketoisomerase/n- acetyltransferase from shewanella denitrificans (see paper)
37% identity, 83% coverage: 19:155/166 of query aligns to 5:145/294 of 4mzuF

query
sites
4mzuF

L

L

V

A

N

D

L

V

Y

Q

G

S

C

Q

T

N

I

I

G

G

S

D

G

N

T

T

R

K

I

V

G
x
W

T
x
Q

F

F

V

C

E

V

I

I

Q

L

K

A

N

G

T

A

T

V

I

I

G

G

R

R

N

N

C

C

K

N

I

I

S
x
C

S

A

H

N

S

S

F

L

L

I

-

E

-

N

-

D

-

V

-

I

-

G

-

D

-

N

-

V

-

T

-

I

-

K

-

S

-

G

-

V

-

Q

-

I

C

W

E

D

G

G

V

I

T

H

L

I

E

Q

D

D

G

D

V

V

F

F

I

I

G

G

H

P

G

N

V

V

M

T

F

F

T

T

N
|
N

D
|
D

L
x
K

Y

Q

P

P

R

R

A

-

I

-

N

-

A

-

D

-

G

-

R

-

Q

-

S

-

E

-

G

-

D

-

W

-

T

S

V

K

V

I

P

K

T

T

R

I

V

V

K

K

Q

K

R

G

V

A

S

S

I

I

G
|
G

S
x
A

N

N

A

S

T

T

I

I

L
|
L

A

P

G

G

I

I

T

L

I

I

G

G

E

E

S

N

A

A

Q

M

V

V

G
|
G

A
|
A

G

G

A

A

V

V

V

I

T
|
T

R
x
K

D

N

V

V

P

P

A

D

Y

N

T

A

I

I

V

V

A
x
I

G

G

V
x
N

P

P

A

G

R
|
R

M

I

I

T

G

G

H

Y

T

V

K

E

binding coenzyme a: N81 (= N77), D82 (= D78), K83 (≠ L79), G100 (= G110), A101 (≠ S111), L106 (= L116), G118 (= G128), A119 (= A129), T124 (= T134), K125 (≠ R135), I134 (≠ A144), N136 (≠ V146), R139 (= R149)
binding thymine: W20 (≠ G34), Q21 (≠ T35), C38 (≠ S52)

Sites not aligning to the query:

binding thymidine-5'-diphosphate: 189, 203, 205, 270

3mqhA Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with coa and udp-3-amino-2-acetamido-2,3-dideoxy glucuronic acid (see paper)
43% identity, 78% coverage: 24:152/166 of query aligns to 32:153/191 of 3mqhA

query
sites
3mqhA

G

G

C

A

T

E

I

I

G

G

S

E

G

G

T

C

R

S

I

L

G

G

T

Q

F

N

V

V

E
x
F

I

V

Q

G

K

N

N

R

T

V

T

R

I

I

G

G

R

N

N

R

C

V

K

K

I

I

S
x
Q

S
x
N

H

N

S

V

F

S

L

V

C
x
Y

E

D

G

N

V

V

T

F

L

L

E

E

D

D

G

D

V

V

F
|
F

I

C

G

G

H

P

G

S

V

M

M

V

F

F

T
|
T

N
|
N

D
x
V

L
x
Y

Y

N

P
|
P

R

R

A

A

I

A

N

I

A

E

D

-

G

-

R
|
R

Q

K

S

S

E

E

G
x
Y

D

R

W

D

T

T

V

I

V

V

P

-

T

-

R

-

V

-

K

R

Q

Q

R

G

V

A

S

T

I

L

G
|
G

S
x
A

N

N

A

C

T

T

I

V

L

V

A

C

G

G

I

A

T

T

I

I

G

G

E

R

S

Y

A

A

Q

F

V

V

G

G

A
|
A

G

G

A

A

V

V

T
x
N

R

K

D

D

V

V

P

P

A

D

Y

F

T

A

I

L

V

V

A
x
V

G

G

V

V

P
|
P

A

A

R
|
R

M

Q

I

I

G

G

binding coenzyme a: F76 (= F68), T84 (= T76), N85 (= N77), V86 (≠ D78), Y87 (≠ L79), P89 (= P81), G111 (= G110), A112 (≠ S111), A130 (= A129), N135 (≠ T134), V145 (≠ A144), P148 (= P147), R150 (= R149)
binding (2S,3S,4R,5R,6R)-5-(acetylamino)-4-amino-6-{[(R)-{[(R)-{[(2R,3S,4R,5R)-5-(2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl]methoxy}(hydroxy)phosphoryl]oxy}(hydroxy)phosphoryl]oxy}-3-hydroxytetrahydro-2H-pyran-2-carboxylic acid: F46 (≠ E38), Q60 (≠ S52), N61 (≠ S53), Y66 (≠ C58), F76 (= F68), N85 (= N77), V86 (≠ D78), R95 (= R90), Y99 (≠ G94)

Sites not aligning to the query:

3mqgC Crystal structure of the 3-n-acetyl transferase wlbb from bordetella petrii in complex with acetyl-coa (see paper)
43% identity, 78% coverage: 24:152/166 of query aligns to 33:154/192 of 3mqgC

query
sites
3mqgC

G

G

C

A

T

E

I

I

G

G

S

E

G

G

T

C

R
x
S

I

L

G

G

T

Q

F

N

V

V

E
x
F

I

V

Q

G

K

N

N

R

T

V

T

R

I

I

G

G

R

N

N

R

C

V

K

K

I

I

S

Q

S
x
N

H

N

S

V

F

S

L

V

C
x
Y

E

D

G

N

V

V

T

F

L

L

E

E

D

D

G

D

V

V

F
|
F

I

C

G

G

H
x
P

G

S

V

M

M

V

F

F

T
|
T

N
|
N

D
x
V

L
x
Y

Y

N

P
|
P

R

R

A

A

I

A

N

I

A

E

D

-

G

-

R

R

Q

K

S

S

E

E

G

Y

D

R

W

D

T

T

V

I

V

V

P

-

T

-

R

-

V

-

K

R

Q

Q

R

G

V

A

S

T

I

L

G
|
G

S
x
A

N

N

A

C

T

T

I

V

L

V

A

C

G

G

I

A

T

T

I

I

G

G

E

R

S

Y

A

A

Q
x
F

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T
x
N

R

K

D

D

V

V

P

P

A

D

Y

F

T

A

I

L

V

V

A
x
V

G

G

V

V

P
|
P

A

A

R
|
R

M

Q

I

I

G

G

binding acetyl coenzyme *a: Y67 (≠ C58), F77 (= F68), P80 (≠ H71), T85 (= T76), N86 (= N77), V87 (≠ D78), Y88 (≠ L79), P90 (= P81), G112 (= G110), A113 (≠ S111), F128 (≠ Q126), G130 (= G128), A131 (= A129), N136 (≠ T134), V146 (≠ A144), P149 (= P147), R151 (= R149)
binding uridine-5'-monophosphate: F47 (≠ E38)
binding uridine-5'-diphosphate: S41 (≠ R32), N62 (≠ S53)

Sites not aligning to the query:

3fsbA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-quinovose (see paper)
37% identity, 75% coverage: 27:151/166 of query aligns to 107:219/260 of 3fsbA

query
sites
3fsbA

I

I

G

G

S

N

G

N

T

V

R

K

I

I

G
|
G

T

T

F

L

V

S

E

D

I

I

Q

Q

K

H

N

H

T

V

T

Y

I

I

G

G

R

N

N

Y

C

V

K

N

I

I

S
x
H

S

S

H

N

S

V

F

F

L

V

C

G

E

E

G

K

V

S

T

I

L

I

E

K

D

D

G

F

V

V

F

W

I

L

G
x
F

H

P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

L

P

Y

T

P

P

R

P

A

S

I

N

N

E

A

L

D

L

G

G

G

-

R

-

Q

-

S

-

E

-

G

-

D

-

W

-

T

-

V

-

P

-

T

V

R

T

V

I

K

E

Q

L

R

F

V

A

S

V

I

I

G
x
A

S
x
A

N

R

A

S

T

V

I

V

L

L

A

P

G

G

I

I

T

H

I

I

G

N

E

E

S

D

A

A

Q

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

R

K

D

D

V

V

P

P

A

K

Y

E

T

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

R

R

M

E

I

I

binding coenzyme a: F150 (≠ G70), A178 (≠ G110), A179 (≠ S111), G196 (= G128), A197 (= A129), V212 (≠ A144)
binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: G114 (= G34), H132 (≠ S52), F150 (≠ G70)

Sites not aligning to the query:

binding [(3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: 78, 241, 243

3fscA Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with coa and dtdp-3-amino-fucose (see paper)
37% identity, 75% coverage: 27:151/166 of query aligns to 107:219/259 of 3fscA

query
sites
3fscA

I

I

G

G

S

N

G

N

T

V

R
x
K

I

I

G
|
G

T
|
T

F

L

V

S

E

D

I

I

Q

Q

K

H

N

H

T

V

T

Y

I

I

G

G

R

N

N

Y

C

V

K

N

I

I

S
x
H

S
|
S

H

N

S

V

F

F

L

V

C

G

E

E

G

K

V

S

T

I

L

I

E

K

D

D

G

F

V

V

F

W

I

L

G
x
F

H

P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

L

P

Y

T

P

P

R

P

A

S

I

N

N

E

A

L

D

L

G

G

G

-

R

-

Q

-

S

-

E

-

G

-

D

-

W

-

T

-

V

-

P

-

T

V

R

T

V

I

K

E

Q

L

R

F

V

A

S

V

I

I

G
x
A

S
x
A

N

R

A

S

T

V

I

V

L

L

A

P

G

G

I

I

T

H

I

I

G

N

E

E

S

D

A

A

Q

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

R

K

D

D

V

V

P

P

A

K

Y

E

T

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

R

R

M

E

I

I

binding coenzyme a: F150 (≠ G70), A178 (≠ G110), A179 (≠ S111), G196 (= G128), A197 (= A129), V212 (≠ A144)
binding (3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-methyloxan-2-yl][hydroxy-[[(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy]phosphoryl] hydrogen phosphate: K112 (≠ R32), G114 (= G34), T115 (= T35), H132 (≠ S52), S133 (= S53)

Sites not aligning to the query:

3fs8A Crystal structure of qdtc, the dtdp-3-amino-3,6-dideoxy-d-glucose n- acetyl transferase from thermoanaerobacterium thermosaccharolyticum in complex with acetyl-coa (see paper)
37% identity, 75% coverage: 27:151/166 of query aligns to 107:219/259 of 3fs8A

query
sites
3fs8A

I

I

G

G

S

N

G

N

T

V

R

K

I

I

G

G

T

T

F

L

V

S

E

D

I

I

Q

Q

K

H

N

H

T

V

T

Y

I

I

G

G

R

N

N

Y

C

V

K

N

I

I

S

H

S

S

H

N

S

V

F

F

L

V

C

G

E

E

G

K

V

S

T

I

L

I

E

K

D

D

G

F

V

V

F

W

I

L

G
x
F

H
x
P

G

H

V

V

M

V

F

L

T

T

N

N

D

D

L

P

Y

T

P

P

R

P

A

S

I

N

N

E

A

L

D

L

G

G

G

-

R

-

Q

-

S

-

E

-

G

-

D

-

W

-

T

-

V

-

P

-

T

V

R

T

V

I

K

E

Q

L

R

F

V

A

S

V

I

I

G
x
A

S
x
A

N

R

A

S

T

V

I

V

L

L

A

P

G

G

I

I

T

H

I

I

G

N

E

E

S

D

A

A

Q

L

V

V

G
|
G

A
|
A

G

G

A

A

V

V

T

T

R

K

D

D

V

V

P

P

A

K

Y

E

T

T

I

V

V

V

A
x
V

G

G

V

N

P

P

A

A

R

R

M

E

I

I

binding acetyl coenzyme *a: F150 (≠ G70), P151 (≠ H71), A178 (≠ G110), A179 (≠ S111), G196 (= G128), A197 (= A129), V212 (≠ A144)

Sites not aligning to the query:

5u2kA Crystal structure of galactoside o-acetyltransferase complex with coa (h3 space group)
29% identity, 66% coverage: 42:151/166 of query aligns to 74:181/190 of 5u2kA

query
sites
5u2kA

N

N

T

V

T

K

I

L

G

G

R

K

N

N

C

V

K

Y

I

V

S

N

S

T

H

N

S

C

F

Y

L

F

C
x
M

E

D

G

G

-

G

-

Q

V

I

T

T

L

I

E

G

D

D

G

N

V

V

F

F

I

I

G

G

H

P

G

N

V

C

M

G

F

F

T
x
Y

N

T

D
x
A

L

T

Y
x
H

P

P

R

L

A

N

I

F

N

H

A

-

D

-

G

-

R

H

Q

R

S

N

E

E

G

G

D

F

W

E

T

K

V

A

V

G

P

P

T

I

R

H

V

I

K

G

Q

S

R

N

V

T

S

W

I

F

G

G

S

G

N

H

A

V

T

A

I

V

L
|
L

A

P

G

G

I

V

T

T

I

I

G

G

E

E

S

G

A

S

Q

V

V

I

G

G

A

A

G

G

A

S

V

V

T
|
T

R
x
K

D

D

V

I

P

P

A

P

Y

H

T

S

I

L

V

A

A

V

G

G

V

N

P

P

A

C

R

K

M

V

I

V

binding coenzyme a: M90 (≠ C58), Y110 (≠ T76), A112 (≠ D78), H114 (≠ Y80), L146 (= L116), T164 (= T134), K165 (≠ R135)

3ectA Crystal structure of the hexapeptide-repeat containing- acetyltransferase vca0836 from vibrio cholerae
33% identity, 67% coverage: 45:156/166 of query aligns to 61:176/176 of 3ectA

query
sites
3ectA

I

F

G

G

R

K

N

T

C

I

K

R

I

I

S

G

S

D

H

H

S

T

F

F

L

I

C

N

E

M

G

N

V

V

T

V

L

M

E

L

D

D

G

G

-

A

-

P

V

I

F

T

I

I

G

G

H

D

G

H

V

V

M

L

F

I

-

G

-

P

T

S

N

T

D

Q

L

F

Y

Y

P

T

R

A

A

S

I

H

N

S

A

L

D

D

G

Y

G

R

R

R

Q

R

S

Q

E

A

G

W

D

E

W

T

T

I

V

C

V

K

P

P

T

I

R

V

V

I

K

E

Q

D

R

D

V

V

S

W

I

I

G

G

S

G

N

N

A

V

T

V

I

I

L

N

A

Q

G
|
G

I

V

T

T

I

I

G

G

E

A

S

R

A

S

Q

V

V

V

G

A

A

A

G

N

A

S

V

V

T

N

R

Q

D
|
D

V

V

P

P

A

P

Y

D

T

T

I

L

V

V

A

G

G

G

V

T

P

P

A

A

R

R

M

I

I

L

G

R

H

S

T

L

K

K

D

D

binding calcium ion: G138 (= G118), D156 (= D136)

3nz2C Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
33% identity, 67% coverage: 45:156/166 of query aligns to 68:183/183 of 3nz2C

query
sites
3nz2C

I

F

G

G

R

K

N

T

C

I

K

R

I

I

S

G

S

D

H

H

S

T

F

F

L

I

C

N

E

M

G

N

V

V

T

V

L

M

E

L

D

D

G

G

-

A

-

P

V

I

F

T

I

I

G

G

H

D

G

H

V

V

M

L

F

I

-

G

-

P

T

S

N

T

D

Q

L

F

Y
|
Y

P

T

R

A

A

S

I
x
H

N

S

A

L

D

D

G

Y

G

R

R

R

Q

R

S

Q

E

A

G

W

D

E

W

T

T

I

V

C

V

K

P

P

T

I

R

V

V

I

K

E

Q

D

R

D

V

V

S

W

I

I

G
|
G

S

G

N

N

A

V

T

V

I

I

L

N

A

Q

G

G

I

V

T

T

I

I

G

G

E

A

S

R

A

S

Q

V

V

V

G
x
A

A
|
A

G
x
N

A

S

V

V

T
x
N

R

Q

D

D

V

V

P

P

A

P

Y

D

T

T

I

L

V

V

A
x
G

G

G

V
x
T

P
|
P

A

A

R

R

M

I

I
x
L

G
x
R

H

S

T

L

K

K

D

D

binding acetyl coenzyme *a: Y107 (= Y80), H111 (≠ I84), G137 (= G110), A155 (≠ G128), A156 (= A129), N161 (≠ T134), G171 (≠ A144), T173 (≠ V146), P174 (= P147), L178 (≠ I151), R179 (≠ G152)
binding magnesium ion: N157 (≠ G130), T173 (≠ V146)

3gvdI Crystal structure of serine acetyltransferase cyse from yersinia pestis
29% identity, 80% coverage: 24:155/166 of query aligns to 120:251/272 of 3gvdI

query
sites
3gvdI

G

G

C

H

T

W

I

L

G

W

S

A

G

Q

T

D

R

R

I

K

G

A

T

L

F

A

V

I

E

Y

I

L

Q

Q

K

N

N

Q

T

V

T

S

I

V

G

A

R

F

N

G

C

V

K

D

I

I

S

H

S

P

H

A

S

A

F

T

L

I

C

G

E

C

G

G

V

I

T

M

L

L

E
x
D

D

H

-

A

-

T

G

G

V

I

F

V

I

I

G

G

H

E

G

T

V

A

M

V

F

V

T

E

N

N

D

D

L

V

-

S

Y

I

P

L

R

Q

A

S

I

V

N

T

A

L

D
x
G

G
|
G

G

T

R

G

Q

K

S

T

E

S

G

G

D

D

W
x
R

T

H

V

-

P

-

T

P

R

K

V

I

K

R

Q

E

R

G

V

V

S

M

I

I

G

G

S

A

N

G

A

A

T

K

I

I

L

L

A

G

G

N

I

I

T

E

I

V

G

G

E

R

S

G

A

A

Q

K

V

I

G

G

A

A

G

G

A

S

V

V

T

L

R

Q

D

S

V

V

P

P

A

A

Y

H

T

T

I

T

V

A

A

A

G

G

V

V

P

P

A

A

R

R

M

I

I

V

G

G

H

K

T

P

K

E

binding cysteine: D160 (≠ E64), G186 (≠ D87), G187 (= G88), R195 (≠ W96)

Sites not aligning to the query:

binding cysteine: 95, 96

3nz2J Crystal structure of hexapeptide-repeat containing-acetyltransferase vca0836 complexed with acetyl co enzyme a from vibrio cholerae o1 biovar eltor
32% identity, 67% coverage: 45:155/166 of query aligns to 71:185/185 of 3nz2J

query
sites
3nz2J

I

F

G

G

R

K

N

T

C

I

K

R

I

I

S

G

S

D

H

H

S

T

F

F

L

I

C

N

E

M

G

N

V

V

T

V

L

M

E

L

D

D

G

G

-

A

-

P

V

I

F

T

I

I

G

G

H

D

G

H

V

V

M

L

F

I

-
x
G

-

P

T

S

N

T

D

Q

L

F

Y
|
Y

P

T

R

A

A

S

I
x
H

N

S

A

L

D

D

G

Y

G

R

R

R

Q

R

S

Q

E

A

G

W

D

E

W

T

T

I

V

C

V

K

P

P

T

I

R

V

V

I

K

E

Q

D

R

D

V

V

S
x
W

I

I

G
|
G

S

G

N

N

A

V

T

V

I

I

L

N

A

Q

G

G

I

V

T

T

I

I

G

G

E

A

S

R

A

S

Q

V

V

V

G
x
A

A
|
A

G

N

A

S

V

V

T
x
N

R

Q

D

D

V

V

P

P

A

P

Y

D

T

T

I

L

V

V

A
x
G

G

G

V
x
T

P
|
P

A

A

R

R

M

I

I

L

G
x
R

H

S

T

L

K

K

binding acetyl coenzyme *a: G104 (vs. gap), Y110 (= Y80), H114 (≠ I84), W138 (≠ S108), G140 (= G110), A158 (≠ G128), A159 (= A129), N164 (≠ T134), G174 (≠ A144), T176 (≠ V146), P177 (= P147), R182 (≠ G152)

1t3dA Crystal structure of serine acetyltransferase from e.Coli at 2.2a (see paper)
31% identity, 69% coverage: 39:152/166 of query aligns to 132:245/262 of 1t3dA

query
sites
1t3dA

I

L

Q

Q

K

N

N

Q

T

V

T

S

I

V

G

T

R

F

N

Q

C

V

K

D

I

I

S

H

S

P

H

A

S

A

F

K

L

I

C

G

E

R

G

G

V

I

T

M

L

L

E
x
D

D

H

-

A

-

T

G

G

V

I

F

V

I

V

G

G

H

E

G

T

V

A

M

V

F

I

T

E

N

N

D

D

L

V

-

S

Y

I

P

L

R

Q

A

S

I

V

N

T

A

L

D

G

G
|
G

G

T

R

G

Q

K

S

S

E

G

G

G

D

D

W
x
R

T
x
H

V

-

P

-

T

P

R

K

V

I

K

R

Q

E

R

G

V

V

S

M

I

I

G

G

S

A

N

G

A

A

T

K

I

I

L

L

A

G

G

N

I

I

T

E

I

V

G

G

E

R

S

G

A

A

Q

K

V

I

G

G

A

A

G

G

A

S

V

V

T

L

R

Q

D

P

V

V

P

P

A

P

Y

H

T

T

I

T

V

A

A

A

G

G

V

V

P

P

A

A

R

R

M

I

I

V

G

G

binding cysteine: D157 (≠ E64), G184 (= G88), R192 (≠ W96), H193 (≠ T97)

Sites not aligning to the query:

binding cysteine: 92, 93

P07464 Galactoside O-acetyltransferase; GAT; Acetyl-CoA:galactoside 6-O-acetyltransferase; Thiogalactoside acetyltransferase; Thiogalactoside transacetylase; EC 2.3.1.18 from Escherichia coli (strain K12) (see 3 papers)
28% identity, 89% coverage: 9:156/166 of query aligns to 40:187/203 of P07464

query
sites
P07464

H

H

D

P

G

S

V

E

V

V

V

E

H

K

H

R

P

E

D

S

L

L

V

I

N

K

L

E

Y

M

G

F

C

A

T

T

I

V

G

G

S

E

G

N

T

A

R

W

I

V

-

E

-

P

G

P

T

V

F

Y

V

F

E
x
S

I

Y

Q

G

K

S

N

N

T

I

T

H

I

I

G

G

R

R

N

N

C

F

K

Y

I

A

S
x
N

S

F

H

N

S

L

F

T

L

I

C

V

E

D

G
x
D

-

Y

-

T

V

V

T

T

L

I

E

G

D

D

G

N

V

V

F

L

I

I

G

A

H

P

G

N

V

V

M

T

F

L

T

S

N

V

D

T

L

G

Y
x
H

P

P

R

-

A

-

I

-

N

-

A

V

D

H

G

H

G

E

R

L

Q

R

S

K

E

N

G

G

D

E

W

M

T

Y

V

S

V

F

P

P

T

I

R

T

V

I

K

G

Q

N

R

N

V

V

S

W

I

I

G

G

S
|
S

N

H

A

V

T

V

I

I

L

N

A

P

G

G

I

V

T

T

I

I

G

G

E

D

S

N

A

S

Q

V

V

I

G

G

A
|
A

G

G

A

S

V

I

V

V

T
|
T

R
x
K

D

D

V

I

P

P

A

P

Y

N

T

V

I

V

V

A

A

A

G

G

V

V

P

P

A

C

R
|
R

M

V

I

I

G
x
R

H

E

T

I

K

N

D

D

S71 (≠ E38) binding substrate
N85 (≠ S52) binding in other chain; binding in other chain
D93 (≠ G60) binding substrate
H115 (≠ Y80) mutation to A: Results in an 1800-fold decrease in catalytic activity.
S142 (= S111) binding in other chain
A160 (= A129) binding in other chain
TK 165:166 (≠ TR 134:135) binding acetyl-CoA
R180 (= R149) binding acetyl-CoA
R183 (≠ G152) binding in other chain

Sites not aligning to the query:

1 modified: Initiator methionine, Removed; Partial
17 binding substrate

Query Sequence

>WP_012061729.1 NCBI__GCF_000017145.1:WP_012061729.1
MIANDVTLHDGVVVHHPDLVNLYGCTIGSGTRIGTFVEIQKNTTIGRNCKISSHSFLCEG
VTLEDGVFIGHGVMFTNDLYPRAINADGGRQSEGDWTVVPTRVKQRVSIGSNATILAGIT
IGESAQVGAGAVVTRDVPAYTIVAGVPARMIGHTKDAAVNTVRAGA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory