SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012067468.1 NCBI__GCF_000017145.1:WP_012067468.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

O23346 Imidazoleglycerol-phosphate dehydratase 2, chloroplastic; IGPD 2; Protein HISTIDINE BIOSYNTHESIS 5B; EC 4.2.1.19 from Arabidopsis thaliana (Mouse-ear cress) (see 2 papers)
49% identity, 97% coverage: 7:202/202 of query aligns to 75:270/272 of O23346

query
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O23346

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E86 (= E18) binding substrate
H112 (= H44) binding Mn(2+)
112:120 (vs. 44:52, 78% identical) binding substrate
H138 (= H70) binding Mn(2+)
HHTNE 138:142 (≠ HHTVE 70:74) binding substrate
H139 (= H71) binding Mn(2+)
E142 (= E74) binding Mn(2+)
R164 (= R96) binding substrate
R186 (= R118) binding substrate
H210 (≠ E142) binding Mn(2+)
H234 (= H166) binding Mn(2+)
234:242 (vs. 166:174, 67% identical) binding substrate
H235 (= H167) binding Mn(2+)
E238 (= E170) binding Mn(2+)
SSK 264:266 (≠ STK 196:198) binding substrate

Sites not aligning to the query:

73 E→Q: Loss of activity.

P34047 Imidazoleglycerol-phosphate dehydratase 1, chloroplastic; IGPD 1; Protein HISTIDINE BIOSYNTHESIS 5A; EC 4.2.1.19 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
49% identity, 97% coverage: 8:202/202 of query aligns to 74:268/270 of P34047

query
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P34047

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H110 (= H44) binding Mn(2+)
H136 (= H70) binding Mn(2+)
H137 (= H71) binding Mn(2+)
E140 (= E74) binding Mn(2+)
H208 (≠ E142) binding Mn(2+)
H232 (= H166) binding Mn(2+)
H233 (= H167) binding Mn(2+)
E236 (= E170) binding Mn(2+)

5el9A A. Thaliana igpd2 in complex with the triazole-phosphonate inhibitor, (s)-c348, to 1.1a resolution (see paper)
49% identity, 97% coverage: 7:202/202 of query aligns to 2:197/199 of 5el9A

query
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binding [(2S)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R96), R113 (= R118), S191 (= S196), K193 (= K198)

5ekwA A. Thaliana igpd2 in complex with the racemate of the triazole- phosphonate inhibitor, c348 (see paper)
50% identity, 96% coverage: 7:199/202 of query aligns to 2:194/194 of 5ekwA

query
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5ekwA

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binding [(2S)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R96), R113 (= R118), S191 (= S196), K193 (= K198)
binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: R91 (= R96), R113 (= R118), S191 (= S196), K193 (= K198)

4qnkA The structure of wt a. Thaliana igpd2 in complex with mn2+ and phosphate (see paper)
49% identity, 91% coverage: 7:190/202 of query aligns to 2:185/185 of 4qnkA

query
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4qnkA

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binding manganese (ii) ion: H39 (= H44), H65 (= H70), E69 (= E74), H137 (≠ E142), H161 (= H166), E165 (= E170)
binding phosphate ion: Q43 (= Q48), H47 (= H52), K169 (= K174)

4mu1A The structure of wt a. Thaliana igpd2 in complex with mn2+, imidazole, and sulfate at 1.5 a resolution (see paper)
49% identity, 91% coverage: 7:190/202 of query aligns to 2:185/185 of 4mu1A

query
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4mu1A

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binding imidazole: H65 (= H70), E69 (= E74)
binding manganese (ii) ion: H65 (= H70), E69 (= E74), H137 (≠ E142)

4mu0A The structure of wt a. Thaliana igpd2 in complex with mn2+ and 1,2,4- triazole at 1.3 a resolution (see paper)
49% identity, 91% coverage: 7:190/202 of query aligns to 2:185/185 of 4mu0A

query
sites
4mu0A

S

A

R

R

T

I

G

G

Q

E

V

V

S

K

R

R

K

E

T

T

N

K

E

E

T

T

A

N

V

V

S

S

V

V

A

K

L

I

D

N

V

L

E

D

G

G

T

H

G

G

S

V

S

S

K

D

I

S

V

S

T

T

G

G

V

I

G

P

F

F

F

L

D

D

H

H

M

M

L

L

D

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

M

V

D

H

I

V

K

R

A

A

E

T

G

G

D

D

L

T

H

H

V

I

D

D

H
|
H

T

T

V

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

Q

T

A

A

L

L

A

L

K

K

A

A

L

L

G

G

D

E

R

R

R

K

G

G

I

I

T

N

R

R

Y

F

A

G

S

D

I

F

D

T

L

A

A

P

M

L

D

D

E

E

T

A

M

L

T

I

R

H

A

V

A

S

V

L

D

D

V

L

S

S

G

G

R

R

P

P

F

Y

L

L

V

G

W

Y

N

N

V

L

A

E

F

I

T

P

A

T

P

Q

K

R

I

V

G

G

T

T

F

Y

D

D

T

T

E

Q

L

L

V

V

R

E

E
x
H

F

F

Q

Q

A

S

L

L

A

V

Q

N

H

T

A

S

G

G

I

M

T

T

L

L

H

H

V

I

Q

R

N

Q

I

L

Y

A

G

G

A

K

N

N

N

S

H

H

I

I

A

I

E

E

T

A

C

T

F

F

K

K

S

A

V

F

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

T

E

E

I

S

D

D

P

P

R

R

Q

R

binding manganese (ii) ion: H65 (= H70), E69 (= E74), H137 (≠ E142)
binding 1,2,4-triazole: H65 (= H70), H66 (= H71)

2f1dA X-ray structure of imidazoleglycerol-phosphate dehydratase (see paper)
49% identity, 90% coverage: 8:189/202 of query aligns to 2:183/183 of 2f1dA

query
sites
2f1dA

R

R

T

I

G

G

Q

E

V

V

S

K

R

R

K

V

T

T

N

K

E

E

T

T

A

N

V

V

S

S

V

V

A

K

L

I

D

N

V

L

E

D

G

G

T

T

G

G

S

V

S

A

K

D

I

S

V

S

T

S

G

G

V

I

G

P

F

F

F

L

D

D

H
|
H

M

M

L

L

D

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

M

V

D

H

I

V

K

R

A

A

E

T

G

G

D

D

L

V

H

H

V

I

D

D

H
|
H

H

H

T

T

V

N

E
|
E

D

D

T

I

G

A

I

L

A

A

I

I

G

G

Q

T

A

A

L

L

A

L

K

K

A

A

L

L

G

G

D

E

R

R

R

K

G

G

I

I

T

N

R

R

Y

F

A

G

S

D

I

F

D

T

L

A

A

P

M

L

D

D

E

E

T

A

M

L

T

I

R

H

A

V

A

S

V

L

D

D

V

L

S

S

G

G

R

R

P

P

F

Y

L

L

V

G

W

Y

N

N

V

L

A

E

F

I

T

P

A

T

P

Q

K

R

I

V

G

G

T

T

F

Y

D

D

T

T

E

Q

L

L

V

V

R

E

E
x
H

F

F

Q

Q

A

S

L

L

A

V

Q

N

H

T

A

S

G

G

I

M

T

T

L

L

H

H

V

I

Q

R

N

Q

I

L

Y

A

G

G

A

E

N

N

N

S

H
|
H

H

H

I

I

A

I

E
|
E

T

A

C

T

F

F

K

K

S

A

V

F

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

T

E

E

I

T

D

D

P

P

R

R

binding manganese (ii) ion: H38 (= H44), H64 (= H70), E68 (= E74), H136 (≠ E142), H160 (= H166), E164 (= E170)

4mu3A The form a structure of an e21q catalytic mutant of a. Thaliana igpd2 in complex with mn2+ and a mixture of its substrate, 2r3s-igp, and an inhibitor, 2s3s-igp, to 1.12 a resolution (see paper)
49% identity, 92% coverage: 7:191/202 of query aligns to 2:186/186 of 4mu3A

query
sites
4mu3A

S

A

R

R

T

I

G

G

Q

E

V

V

S

K

R

R

K

E

T

T

N

K

E
x
Q

T

T

A

N

V

V

S

S

V

V

A

K

L

I

D

N

V

L

E

D

G

G

T

H

G

G

S

V

S

S

K

D

I

S

V

S

T

T

G

G

V

I

G

P

F

F

F

L

D

D

H

H

M

M

L

L

D

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

M

V

D

H

I

V

K

R

A

A

E

T

G

G

D

D

L

T

H

H

V

I

D

D

H
|
H

T

T

V

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

Q

T

A

A

L

L

A

L

K

K

A

A

L

L

G

G

D

E

R

R

R

K

G

G

I

I

T

N

R

R

Y

F

A

G

S

D

I

F

D

T

L

A

A

P

M

L

D

D

E

E

T

A

M

L

T

I

R

H

A

V

A

S

V

L

D

D

V

L

S

S

G

G

R

R

P

P

F

Y

L

L

V

G

W

Y

N

N

V

L

A

E

F

I

T

P

A

T

P

Q

K

R

I

V

G

G

T

T

F

Y

D

D

T

T

E

Q

L

L

V

V

R

E

E
x
H

F

F

Q

Q

A

S

L

L

A

V

Q

N

H

T

A

S

G

G

I

M

T

T

L

L

H

H

V

I

Q

R

N

Q

I

L

Y

A

G

G

A

K

N

N

N

S

H

H

I

I

A

I

E

E

T

A

C

T

F

F

K

K

S

A

V

F

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

T

E

E

I

S

D

D

P

P

R

R

Q

R

A

G

binding (2S,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H65 (= H70), H66 (= H71)
binding (2R,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: Q13 (≠ E18), H65 (= H70), H66 (= H71)
binding manganese (ii) ion: H65 (= H70), E69 (= E74), H137 (≠ E142)

8qavA Medicago truncatula hisn5 (igpd) in complex with mn and ig2 (see paper)
47% identity, 91% coverage: 7:190/202 of query aligns to 1:184/184 of 8qavA

query
sites
8qavA

S

A

R

R

T

I

G

G

Q

E

V

M

S

K

R

R

K

V

T

T

N

K

E

E

T

T

A

N

V

V

S

S

V

V

A

K

L

I

D

N

V

L

E

D

G

G

T

T

G

G

S

V

S

A

K

D

I

N

V

S

T

S

G

G

V

I

G

P

F

F

F

L

D

D

H
|
H

M

M

L

L

D

D

Q

Q

L

L

S

A

R

S

H

H

S

G

L

L

I

F

D

D

M

V

D

H

I

V

K

K

A

A

E

T

G

G

D

D

L

T

H

H

V

I

D

D

H
|
H

T

T

V

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

Q

T

A

A

L

L

A

L

K

Q

A

A

L

L

G

G

D

D

R

R

R

K

G

G

I

I

T

N

R

R

Y

F

A

G

S

N

I

F

D

S

L

A

A

P

M

L

D

D

E

E

T

A

M

L

T

V

R

H

A

V

A

S

V

L

D

D

V

L

S

S

G

G

R

R

P

P

F

H

L

L

V

G

W

Y

N

D

V

L

A

N

F

I

T

P

A

T

P

Q

K

R

I

V

G

G

T

K

F

Y

D

D

T

T

E

Q

L

L

V

V

R

E

E
x
H

F

F

Q

Q

A

S

L

L

A

V

Q

N

H

T

A

S

G

G

I

M

T

T

L

L

H

H

V

I

Q

R

N

Q

I

F

Y

S

G

G

A

T

N

N

N

S

H
|
H

H

H

I

I

A

I

E
|
E

T

A

C

T

F

F

K

K

S

A

V

F

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

T

E

E

I

Y

D

D

P

T

R

R

Q

R

binding (2S,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H64 (= H70), H65 (= H71)
binding manganese (ii) ion: H38 (= H44), H64 (= H70), E68 (= E74), H136 (≠ E142), H160 (= H166), E164 (= E170)

8qawA Medicago truncatula hisn5 (igpd) in complex with mn, imd, edo, fmt, gol and trs (see paper)
47% identity, 91% coverage: 7:190/202 of query aligns to 2:185/185 of 8qawA

query
sites
8qawA

S

A

R

R

T

I

G

G

Q

E

V

M

S

K

R

R

K

V

T

T

N

K

E
|
E

T

T

A

N

V

V

S

S

V

V

A

K

L

I

D

N

V

L

E

D

G

G

T

T

G

G

S

V

S

A

K

D

I

N

V

S

T

S

G

G

V

I

G

P

F

F

F

L

D

D

H
|
H

M

M

L

L

D

D

Q
|
Q

L

L

S

A

R

S

H
|
H

S

G

L

L

I

F

D

D

M

V

D

H

I

V

K

K

A

A

E

T

G

G

D

D

L

T

H

H

V

I

D

D

H
|
H

T

T

V

N

E
|
E

D

D

T

V

G

A

I

L

A

A

I

I

G

G

Q

T

A

A

L

L

A

L

K

Q

A

A

L

L

G

G

D

D

R

R

R

K

G

G

I

I

T

N

R

R

Y

F

A

G

S

N

I

F

D

S

L

A

A

P

M

L

D

D

E

E

T

A

M

L

T

V

R

H

A

V

A

S

V

L

D

D

V

L

S

S

G

G

R

R

P

P

F

H

L

L

V

G

W

Y

N

D

V

L

A

N

F

I

T

P

A

T

P

Q

K

R

I

V

G

G

T

K

F

Y

D

D

T

T

E

Q

L

L

V

V

R

E

E
x
H

F

F

Q

Q

A

S

L

L

A

V

Q

N

H

T

A

S

G

G

I

M

T

T

L

L

H

H

V

I

Q

R

N

Q

I

F

Y

S

G

G

A

T

N

N

N

S

H
|
H

H

H

I

I

A

I

E
|
E

T

A

C

T

F

F

K
|
K

S

A

V

F

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

T

E

E

I

Y

D

D

P

T

R

R

Q

R

binding glycerol: Q43 (= Q48), H47 (= H52), K169 (= K174)
binding imidazole: E13 (= E18), H66 (= H71), E69 (= E74)
binding manganese (ii) ion: H39 (= H44), H65 (= H70), E69 (= E74), H137 (≠ E142), H161 (= H166), E165 (= E170)

6fwhA Acanthamoeba igpd in complex with r-c348 to 1.7a resolution (see paper)
48% identity, 96% coverage: 8:201/202 of query aligns to 3:195/196 of 6fwhA

query
sites
6fwhA

R

R

T

E

G

A

Q

Q

V

V

S

A

R

R

K

E

T

T

N

G

E
|
E

T

T

A

K

V

I

S

E

V

V

A

R

L

L

D

S

V

L

E

D

G

G

T

T

G

G

S

V

S

S

K

D

I

V

V

K

T

T

G

G

V

I

G

G

F

F

F

L

D

D

H
|
H

M

M

L

L

D

S

Q

A

L

L

S

A

R

K

H

H

S

G

L

R

I

F

D

D

M

L

D

Y

I

L

K

R

A

C

E

A

G

G

D

D

L

L

H

H

V

V

D

D

H
|
H

T

T

V

S

E
|
E

D

D

T

C

G

A

I

I

A

V

I

L

G

G

Q

Q

A

A

L

F

A

R

K

Q

A

A

L

I

G

G

D

E

R

R

R

K

G

G

I

I

T

K

R
|
R

Y

Y

A

G

S

S

I

A

D

Y

L

A

A

P

M

L

D

D

E

E

T

S

M

L

T

A

R

R

A

A

A

V

V

V

D

D

V

I

S

S

G

S

R
|
R

P

P

F

F

L

A

V

V

W

I

N

D

V

L

A

K

F

L

T

K

A

R

P

E

K

K

I

I

G

G

T

E

F

L

D

S

T

C

E

E

L

M

V

I

R

P

E
x
H

F

V

F

L

Q

H

A

S

L

F

A

A

Q

T

H

S

A

A

G

N

I

L

T

T

L

L

H

H

V

V

Q

E

N

V

I

L

Y

Y

G

G

A

A

N

N

N

D

H
|
H

I

K

A

A

E
|
E

T

S

C

A

F

F

K

K

S

A

V

T

A

A

R

L

V

A

L

L

R

R

T

E

A

A

T

V

E

T

I

K

D

D

-

G

P

P

R

A

Q

D

A

A

G

-

R

-

V

V

P

P

S
|
S

T

T

K
|
K

G

G

T

V

L

L

binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: E13 (= E18), H39 (= H44), H65 (= H70), H66 (= H71), E69 (= E74), R91 (= R96), R113 (= R118), H161 (= H166), H162 (= H167), E165 (= E170), S190 (= S196), K192 (= K198)
binding manganese (ii) ion: H39 (= H44), H65 (= H70), E69 (= E74), H137 (≠ E142), H161 (= H166), E165 (= E170)

6fwhL Acanthamoeba igpd in complex with r-c348 to 1.7a resolution (see paper)
48% identity, 96% coverage: 8:201/202 of query aligns to 2:194/195 of 6fwhL

query
sites
6fwhL

R

R

T

E

G

A

Q
|
Q

V

V

S

A

R

R

K

E

T

T

N

G

E
|
E

T

T

A

K

V

I

S

E

V

V

A

R

L

L

D

S

V

L

E

D

G

G

T

T

G

G

S

V

S

S

K

D

I

V

V

K

T

T

G

G

V

I

G

G

F

F

F

L

D

D

H
|
H

M

M

L

L

D

S

Q

A

L

L

S

A

R

K

H

H

S

G

L

R

I

F

D

D

M

L

D

Y

I

L

K

R

A

C

E

A

G

G

D

D

L

L

H

H

V

V

D

D

H
|
H

T

T

V

S

E
|
E

D

D

T

C

G

A

I

I

A

V

I

L

G

G

Q

Q

A

A

L

F

A

R

K
x
Q

A

A

L

I

G

G

D

E

R

R

R

K

G

G

I

I

T

K

R
|
R

Y

Y

A

G

S

S

I

A

D

Y

L

A

A

P

M

L

D

D

E

E

T

S

M

L

T

A

R

R

A

A

A

V

V

V

D

D

V

I

S

S

G

S

R
|
R

P

P

F

F

L

A

V

V

W

I

N

D

V

L

A

K

F

L

T

K

A

R

P

E

K

K

I

I

G

G

T

E

F

L

D

S

T

C

E

E

L

M

V

I

R

P

E
x
H

F

V

F

L

Q

H

A

S

L

F

A

A

Q

T

H

S

A

A

G

N

I

L

T

T

L

L

H

H

V

V

Q

E

N

V

I

L

Y

Y

G

G

A

A

N

N

N

D

H
|
H

I

K

A

A

E
|
E

T

S

C

A

F

F

K

K

S

A

V

T

A

A

R

L

V

A

L

L

R

R

T

E

A

A

T

V

E

T

I

K

D

D

-

G

P

P

R

A

Q

D

A

A

G

-

R

-

V

V

P

P

S
|
S

T

T

K
|
K

G

G

T

V

L

L

binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: E12 (= E18), H38 (= H44), H64 (= H70), H65 (= H71), E68 (= E74), R90 (= R96), R112 (= R118), H160 (= H166), H161 (= H167), E164 (= E170), S189 (= S196), K191 (= K198)
binding magnesium ion: Q5 (= Q11), Q80 (≠ K86)
binding manganese (ii) ion: H38 (= H44), H64 (= H70), E68 (= E74), H136 (≠ E142), H160 (= H166), E164 (= E170)

Q9S5G5 Histidine biosynthesis bifunctional protein HisB; EC 3.1.3.15; EC 4.2.1.19 from Escherichia coli O157:H7 (see paper)
47% identity, 96% coverage: 8:201/202 of query aligns to 167:355/355 of Q9S5G5

query
sites
Q9S5G5

R

R

T

Y

G

A

Q

H

V

V

S

V

R

R

K

N

T

T

N

K

E

E

T

T

A

Q

V

I

S

D

V

V

A

Q

L

V

D

W

V

L

E

D

G

R

T

E

G

G

S

G

S

S

K

K

I

I

V

N

T

T

G

G

V

V

G

G

F

F

D

D

H

H

M

M

L

L

D

D

Q

Q

L

I

S

A

R

T

H

H

S

G

L

G

I

F

D

R

M

M

D

E

I

I

K

N

A

V

E

K

G

G

D

D

L

L

H

Y

V

I

D

D

H

H

T

T

V

V

E

E

D

D

T

T

G

G

I

L

A

A

I

L

G

G

Q

E

A

A

L

L

A

K

K

I

A

A

L

L

G

G

D

D

R

K

R

R

G

G

I

I

T

C

R

R

Y

F

A

G

S

F

I

V

D

-

L

L

A

P

M

M

D

D

E

E

T

C

M

L

T

A

R

R

A

C

A

A

V

L

D

D

V

I

S

S

G

G

R

R

P

P

F

H

L

L

V

E

W

Y

N

K

V

A

A

E

F

F

T

T

A

Y

P

Q

K

R

I

V

G

G

T

D

F

L

D

S

T

T

E

E

L

M

V

I

R

E

E

H

F

F

Q

R

A

S

L

L

A

S

Q

Y

H

T

A

M

G

G

I

V

T

T

L

L

H

H

V

L

Q

K

N

T

I

-

Y

K

G

G

A

K

N

N

N

D

H

H

I

R

A

V

E

E

T

S

C

L

F

F

K

K

S

A

V

F

A

G

R

R

V

T

L

L

R

R

T

Q

A

A

T

I

E

R

I

V

D

-

P

-

R

-

Q

E

A

G

G

D

R

T

V

L

P

P

S

S

T

S

K

K

G

G

T

V

L

L

Sites not aligning to the query:

1:166 Histidinol-phosphatase
9 binding Mg(2+)
11 binding Mg(2+)
17 E→A: Severe decrease in histidinol-phosphate phosphatase activity in presence of low magnesium concentration. At higher magnesium concentration (5mM), no effect on activity.
93 binding Zn(2+)
95 binding Zn(2+)
101 binding Zn(2+)
103 binding Zn(2+)
130 binding Mg(2+)

P9WML9 Imidazoleglycerol-phosphate dehydratase; IGPD; EC 4.2.1.19 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
46% identity, 91% coverage: 18:201/202 of query aligns to 21:210/210 of P9WML9

query
sites
P9WML9

E

E

T

S

A

D

V

I

S

V

V

I

A

E

L

L

D

D

V

L

E

D

G

G

T

T

G

G

S

Q

S

V

K

A

I

V

V

D

T

T

G

G

V

V

G

P

F

F

F

Y

D

D

H
|
H

M
|
M

L
|
L

D
x
T

Q
x
A

L
|
L

S
x
G

R
x
S

H
|
H

S

A

L

S

I

F

D

D

M

L

D

T

I

V

K

R

A

A

E

T

G

G

D

D

L

V

H

E

V

I

D

E

D

A

H
|
H

T
|
T

V
x
I

E
|
E

D

D

T

T

G

A

I

I

A

A

I

L

G

G

Q

T

A

A

L

L

A

G

K

Q

A

A

L

L

G

G

D

D

R

K

R

R

G

G

I

I

T

R

R

R

Y

F

A

G

S

D

I

A

D

F

L

I

A

P

M

M

D

D

E

E

T

T

M

L

T

A

R

H

A

A

V

V

D

D

V

L

S

S

G

G

R

R

P

P

F

Y

L

C

V

V

-

H

-

T

-

G

-

E

-

P

-

D

-

H

W

L

N

Q

V

H

A

T

F

T

T

I

A

A

P

G

K

S

I

S

G

V

T

P

F

Y

D

H

T

T

E

V

L

I

V

N

R

R

E
x
H

F

V

F

F

Q

E

A

S

L

L

A

A

Q

A

H

N

A

A

G

R

I

I

T

A

L

L

H

H

V

V

Q

R

N

V

I

L

Y

Y

G

G

A

R

N

D

N

P

H
|
H

I
|
I

A
x
T

E
|
E

T
x
A

C
x
Q

F
x
Y

K
|
K

S

A

V

V

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

V

E

E

I

P

D

D

P

P

R

R

Q

V

A

S

G

G

R

-

V

V

P

P

S

S

T

T

K

K

G

G

T

A

L

L

H47 (= H44) binding Mn(2+)
47:55 (vs. 44:52, 56% identical) binding substrate
H73 (= H70) binding Mn(2+)
HHTIE 73:77 (≠ HHTVE 70:74) binding substrate
H74 (= H71) binding Mn(2+)
E77 (= E74) binding Mn(2+)
H152 (≠ E142) binding Mn(2+)
H176 (= H166) binding Mn(2+)
176:184 (vs. 166:174, 56% identical) binding substrate
H177 (= H167) binding Mn(2+)
E180 (= E170) binding Mn(2+)

6ezmU Imidazoleglycerol-phosphate dehydratase from saccharomyces cerevisiae (see paper)
39% identity, 94% coverage: 12:201/202 of query aligns to 6:212/212 of 6ezmU

query
sites
6ezmU

V

V

S

K

R

R

K

I

T

T

N

N

E

E

T

T

A

K

V

I

S

Q

V

I

A

A

L

I

D

S

V

L

E

K

G

G

-

G

-

P

-

L

-

A

-

I

-

E

-

H

-

S

-

I

-

F

-

P

-

E

-

K

-

A

-

E

-

Q

T

A

G

T

S

Q

S

S

K

Q

I

V

V

I

-

N

-

V

-

H

T

T

G

G

V

I

G

G

F

F

F

L

D

D

H
|
H

M

M

L

I

D

H

Q

A

L

L

S

A

R

K

H

H

S

S

L

G

I

W

D

S

M

L

D

I

I

V

K

E

A

C

E

I

G

G

D

D

L

L

H

H

V

I

D

D

H
|
H

T

T

V

T

E
|
E

D

D

T

C

G

G

I

I

A

A

I

L

G

G

Q

Q

A

A

L

F

A

K

K

E

A

A

L

L

G

G

D

A

R

V

R

R

G

G

I

V

T

K

R

R

Y

F

A

G

S

S

I

G

D

F

L

A

A

P

M

L

D

D

E

E

T

A

M

L

T

S

R

R

A

A

A

V

V

V

D

D

V

L

S

S

G

N

R

R

P

P

F

Y

L

A

V

V

W

V

N

E

V

L

A

G

F

L

T

Q

A

R

P

E

K

K

I

V

G

G

T

D

F

L

D

S

T

C

E

E

L

M

V

I

R

P

E
x
H

F

F

F

L

Q

E

A

S

L

F

A

A

Q

E

H

A

A

S

G

R

I

I

T

T

L

L

H

H

V

V

Q

D

N

C

I

L

Y

R

G

G

A

K

N

N

N

D

H
|
H

I

R

A

S

E
|
E

T

S

C

A

F

F

K
|
K

S

A

V

L

A

A

R

V

V

A

L

I

R

R

T

E

A

A

T

T

E

-

I

-

D

S

P

P

R

N

Q

G

A

T

G

N

R

D

V

V

P

P

S
|
S

T

T

K
|
K

G

G

T

V

L

L

binding [(2R)-2-hydroxy-3-(1H-1,2,4-triazol-1-yl)propyl]phosphonic acid: H57 (= H44), H83 (= H70), H84 (= H71), E87 (= E74), H179 (= H166), H180 (= H167), E183 (= E170), K187 (= K174), S207 (= S196), K209 (= K198)
binding manganese (ii) ion: H57 (= H44), H83 (= H70), E87 (= E74), H155 (≠ E142), H179 (= H166), E183 (= E170)

P06633 Imidazoleglycerol-phosphate dehydratase; IGPD; EC 4.2.1.19 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see paper)
38% identity, 94% coverage: 12:201/202 of query aligns to 8:219/220 of P06633

query
sites
P06633

V

V

S

K

R

R

K

I

T

T

N

N

E

E

T

T

A

K

V

I

S

Q

V

I

A

A

L

I

D

S

V

L

E

K

G

G

-

G

-

P

-

L

-

A

-

I

-

E

-

H

-

S

-

I

-

F

-

P

-

E

-

K

-

E

-

A

-

E

-

A

-

V

-

A

-

E

-

Q

T

A

G

T

S

Q

S

S

K

Q

I

V

V

I

-

N

-

V

-

H

T

T

G

G

V

I

G

G

F

F

F

L

D

D

H
|
H

M
|
M

L
x
I

D
x
H

Q
x
A

L
|
L

S
x
A

R
x
K

H
|
H

S

S

L

G

I

W

D

S

M

L

D

I

I

V

K

E

A

C

E

I

G

G

D

D

L

L

H

H

V

I

D

D

H
|
H

T
|
T

V
x
T

E
|
E

D

D

T

C

G

G

I

I

A

A

I

L

G

G

Q

Q

A

A

L

F

A

K

K

E

A

A

L

L

G

G

D

A

R

V

R

R

G

G

I

V

T

K

R
|
R

Y

F

A

G

S

S

I

G

D

F

L

A

A

P

M

L

D

D

E

E

T

A

M

L

T

S

R

R

A

A

A

V

V

V

D

D

V

L

S

S

G

N

R

R

P

P

F

Y

L

A

V

V

W

V

N

E

V

L

A

G

F

L

T

Q

A

R

P

E

K

K

I

V

G

G

T

D

F

L

D

S

T

C

E

E

L

M

V

I

R

P

E
x
H

F

F

F

L

Q

E

A

S

L

F

A

A

Q

E

H

A

A

S

G

R

I

I

T

T

L

L

H

H

V

V

Q

D

N

C

I

L

Y

R

G

G

A

K

N

N

N

D

H
|
H

I
x
R

A
x
S

E
|
E

T
x
S

C
x
A

F
|
F

K
|
K

S

A

V

L

A

A

R

V

V

A

L

I

R

R

T

E

A

A

T

T

E

-

I

-

D

S

P

P

R

N

Q

G

A

T

G

N

R

D

V

V

P

P

S
|
S

T
|
T

K
|
K

G

G

T

V

L

L

H64 (= H44) binding Mn(2+)
64:72 (vs. 44:52, 44% identical) binding substrate
H90 (= H70) binding Mn(2+)
HHTTE 90:94 (≠ HHTVE 70:74) binding substrate
H91 (= H71) binding Mn(2+)
E94 (= E74) binding Mn(2+)
R116 (= R96) binding substrate
H162 (≠ E142) binding Mn(2+)
H186 (= H166) binding Mn(2+)
186:194 (vs. 166:174, 56% identical) binding substrate
H187 (= H167) binding Mn(2+)
E190 (= E170) binding Mn(2+)
STK 214:216 (= STK 196:198) binding substrate

5xdsA Crystal structure of mycobacterium tuberculosis hisb bound with an inhibitor (see paper)
45% identity, 85% coverage: 18:189/202 of query aligns to 12:190/191 of 5xdsA

query
sites
5xdsA

E

E

T

S

A

D

V

I

S

V

V

I

A

E

L

L

D

D

V

L

E

D

G

G

T

T

G

G

S

Q

S

V

K

A

I

V

V

D

T

T

G

G

V

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

D

T

Q

A

L

L

S

G

R

S

H

H

S

A

L

S

I

F

D

D

M

L

D

T

I

V

K

R

A

A

E

T

G

G

D

D

L

V

H

E

V

I

D

E

D

A

H
|
H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

A

A

I

L

G

G

Q

T

A

A

L

L

A

G

K

Q

A

A

L

L

G

G

D

D

R

K

R

R

G

G

I

I

T

R

R

R

Y

F

A

G

S

D

I

A

D

F

L

I

A

P

M

M

D

D

E

E

T

T

M

L

T

A

R

H

A

A

V

V

D

D

V

L

S

S

G

G

R

R

P

P

F

Y

L

C

V

V

-

H

-

T

-

G

-

E

-

P

-

D

-

H

W

L

N

Q

V

H

A

T

F

T

T

I

A

A

P

G

K

S

I

S

G

V

T

P

F

Y

D

H

T

T

E

V

L

I

V

N

R

R

E
x
H

F

V

F

F

Q

E

A

S

L

L

A

A

Q

A

H

N

A

A

G

R

I

I

T

A

L

L

H

H

V

V

Q

R

N

V

I

L

Y

Y

G

G

A

R

N

D

N

P

H
|
H

H

H

I

I

A

T

E
|
E

T

A

C

Q

F

Y

K

K

S

A

V

V

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

V

E

E

I

P

D

D

P

P

R

R

binding (2S)-2-azanyl-3-(4H-1,2,4-triazol-3-yl)propanoic acid: H64 (= H70), H65 (= H71), E68 (= E74)
binding manganese (ii) ion: H38 (= H44), H64 (= H70), E68 (= E74), H143 (≠ E142), H167 (= H166), E171 (= E170)

4lomA Crystal structure of mycobacterium tuberculosis hisb in complex with its substrate
45% identity, 85% coverage: 18:189/202 of query aligns to 12:190/191 of 4lomA

query
sites
4lomA

E

E

T

S

A

D

V

I

S

V

V

I

A

E

L

L

D

D

V

L

E

D

G

G

T

T

G

G

S

Q

S

V

K

A

I

V

V

D

T

T

G

G

V

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

D

T

Q

A

L

L

S

G

R

S

H

H

S

A

L

S

I

F

D

D

M

L

D

T

I

V

K

R

A

A

E

T

G

G

D

D

L

V

H

E

V

I

D

E

D

A

H
|
H

H

H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

A

A

I

L

G

G

Q

T

A

A

L

L

A

G

K

Q

A

A

L

L

G

G

D

D

R

K

R

R

G

G

I

I

T

R

R

R

Y

F

A

G

S

D

I

A

D

F

L

I

A

P

M
|
M

D

D

E

E

T

T

M

L

T

A

R

H

A

A

V

V

D

D

V

L

S

S

G

G

R

R

P

P

F

Y

L

C

V

V

-

H

-

T

-

G

-

E

-

P

-

D

-

H

W

L

N

Q

V

H

A

T

F

T

T

I

A

A

P

G

K

S

I

S

G

V

T

P

F

Y

D

H

T

T

E

V

L

I

V

N

R

R

E
x
H

F

V

F

F

Q

E

A

S

L

L

A

A

Q

A

H

N

A

A

G

R

I

I

T

A

L

L

H

H

V

V

Q

R

N

V

I

L

Y

Y

G

G

A

R

N

D

N

P

H
|
H

I

I

A

T

E
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E

T

A

C

Q

F

Y

K
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K

S

A

V

V

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

V

E

E

I

P

D

D

P

P

R

R

binding (2R,3S)-2,3-dihydroxy-3-(1H-imidazol-5-yl)propyl dihydrogen phosphate: H38 (= H44), M98 (= M104), H167 (= H166), H168 (= H167), E171 (= E170), K175 (= K174)
binding manganese (ii) ion: H38 (= H44), H64 (= H70), E68 (= E74), H143 (≠ E142), H167 (= H166), E171 (= E170)

4gquA Crystal structure of hisb from mycobacterium tuberculosis
45% identity, 85% coverage: 18:189/202 of query aligns to 12:190/191 of 4gquA

query
sites
4gquA

E

E

T

S

A

D

V

I

S

V

V

I

A

E

L

L

D

D

V

L

E

D

G

G

T

T

G

G

S

Q

S

V

K

A

I

V

V

D

T

T

G

G

V

V

G

P

F

F

F

Y

D

D

H
|
H

M

M

L

L

D

T

Q

A

L

L

S

G

R

S

H

H

S

A

L

S

I

F

D

D

M

L

D

T

I

V

K

R

A

A

E

T

G

G

D

D

L

V

H

E

V

I

D

E

D

A

H
|
H

H

H

T

T

V

I

E
|
E

D

D

T

T

G

A

I

I

A

A

I

L

G

G

Q

T

A

A

L

L

A

G

K

Q

A

A

L

L

G

G

D

D

R

K

R

R

G

G

I

I

T

R

R

R

Y

F

A

G

S

D

I

A

D

F

L

I

A

P

M

M

D

D

E

E

T

T

M

L

T

A

R

H

A

A

V

V

D

D

V

L

S

S

G

G

R

R

P

P

F

Y

L

C

V

V

-

H

-

T

-

G

-

E

-

P

-

D

-

H

W

L

N

Q

V

H

A

T

F

T

T

I

A

A

P

G

K

S

I

S

G

V

T

P

F

Y

D

H

T

T

E

V

L

I

V

N

R

R

E
x
H

F

V

F

F

Q

E

A

S

L

L

A

A

Q

A

H

N

A

A

G

R

I

I

T

A

L

L

H

H

V

V

Q

R

N

V

I

L

Y

Y

G

G

A

R

N

D

N

P

H
|
H

H

H

I

I

A

T

E
|
E

T

A

C

Q

F

Y

K

K

S

A

V

V

A

A

R

R

V

A

L

L

R

R

T

Q

A

A

T

V

E

E

I

P

D

D

P

P

R

R

binding manganese (ii) ion: H38 (= H44), H64 (= H70), E68 (= E74), H143 (≠ E142), H167 (= H166), E171 (= E170)

Query Sequence

>WP_012067468.1 NCBI__GCF_000017145.1:WP_012067468.1
MADVTPSRTGQVSRKTNETAVSVALDVEGTGSSKIVTGVGFFDHMLDQLSRHSLIDMDIK
AEGDLHVDDHHTVEDTGIAIGQALAKALGDRRGITRYASIDLAMDETMTRAAVDVSGRPF
LVWNVAFTAPKIGTFDTELVREFFQALAQHAGITLHVQNIYGANNHHIAETCFKSVARVL
RTATEIDPRQAGRVPSTKGTLA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory