SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012067598.1 NCBI__GCF_000017145.1:WP_012067598.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
46% identity, 96% coverage: 11:257/258 of query aligns to 64:303/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
54% identity, 95% coverage: 14:258/258 of query aligns to 6:248/271 of Q72J02

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Q72J02

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C192 (= C202) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
45% identity, 93% coverage: 15:254/258 of query aligns to 65:309/482 of O59954

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O59954

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G82 (= G32) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G50) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R80) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G135) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ D165) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G213) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
51% identity, 95% coverage: 10:254/258 of query aligns to 4:240/270 of D4GSF3

query
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D4GSF3

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C188 (= C202) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
44% identity, 92% coverage: 11:247/258 of query aligns to 39:272/440 of P38820

query
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P38820

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L

L

L

M

L

L

Y

Y

D

S

A

G

L

F

A

P

A

Q

Q

Q

C225 (= C202) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
48% identity, 93% coverage: 9:248/258 of query aligns to 53:285/460 of O95396

query
sites
O95396

A

A

T

A

L

L

A

S

P

R

S

D

E

E

I

I

A

L

R

R

Y

Y

A

S

R

R

H

Q

I

L

V

V

L

L

P

P

E

E

I

L

G

G

G

V

P

H

G

G

Q

Q

Q

L

K

R

L

L

K

G

A

T

A

A

R

C

V

V

L

L

V

I

I

V

G

G

A

C

G

G

L

L

G

G

A

C

P

P

V

L

L

A

Q

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

T

R

L

L

G

G

I

L

V

V

D

D

D

Y

D

D

V

V

S

E

L

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

V

V

I

L

H

H

A

G

T

E

E

A

D

L

I

A

G

G

R

Q

A

A

K

K

V

A

E

F

S

S

A

A

A

S

I

L

D

R

A

R

L

L

N

N

P

S

H

A

V

V

K

E

V

C

I

V

T

P

H

Y

A
x
T

L
x
Q

R
x
A

L
|
L

G
x
T

A
x
P

E
x
A

N
x
T

A
|
A

R
x
L

E
x
D

L
|
L

F
x
V

D
x
R

A
x
R

Y
|
Y

D
|
D

L
x
V

V
|
V

V
x
A

D
|
D

G
x
C

S
|
S

D
|
D

N
|
N

F
x
V

D
x
P

T
|
T

R
|
R

Y
|
Y

L
|
L

A
x
V

A
x
N

D
|
D

T
x
A

A
x
C

E
x
V

A
x
L

A
|
A

A
x
G

V
x
R

A
x
P

L
|
L

V
|
V

T
x
S

G
x
A

A
x
S

V
x
A

G
x
L

R
|
R

F
|
F

D
x
E

G
|
G

S
x
Q

V
x
I

T
|
T

V
|
V

L

-

K

-

P

-

Y
|
Y

E
x
H

T
x
Y

D
|
D

A
x
G

D

-

G

-

H

-

P

-

N
x
G

P
|
P

S
x
C

Y
|
Y

R
|
R

D
x
C

L
x
I

F
|
F

P
|
P

E
x
Q

P
|
P

P
x
A

G
x
E

A
x
T

V
|
V

P
x
T

S
x
N

C
|
C

A

A

E

D

A

G

G

G

V

V

L

L

G

G

A

V

L

V

T

T

G

G

V

V

I

L

G

G

T

C

L

L

Q

Q

A

A

M

L

E

E

A

V

I

L

K

K

L

I

V

A

T

A

G

G

I

L

G

G

E

P

P

S

L

Y

I

S

G

G

R

S

L

L

Y

F

D

D

A

A

L

L

A

R

A

G

Q

H

F

F

158:238 (vs. 114:201, 39% identical) Interaction with NFS1
C239 (= C202) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
45% identity, 96% coverage: 9:255/258 of query aligns to 2:240/249 of P12282

query
sites
P12282

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

A
x
C

P

A

V

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D

D

D

F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

A

S

T

D

E

A

D

T

I

V

G

G

R

Q

A

P

K

K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

I

T

-

P

T

V

H

N

A

A

L

L

R

L

L

D

G

D

A

A

E

E

N

L

A

A

R

-

E

A

L

L

F

I

D

A

A

E

Y

H

D

D

L

L

V

V

V

L

D

D

G
x
C

S

T

D
|
D

N

N

F

V

D

A

T

V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A
x
C

E

F

A

A

A

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

E

-

T

T

D

Y

A

Q

D

D

G

G

H

E

P

-

N

-

P

P

S
x
C

Y

Y

R

R

-
x
C

-

L

-

S

D

R

L

L

F

F

P

G

E

E

P

N

P

A

P

-

G

-

A

-

V

-

P

L

S

T

C
|
C

A

V

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A
x
C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

R14 (= R21) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ A51) mutation to A: No effect.
R73 (= R80) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G135) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D137) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (≠ A149) mutation to A: No effect.
C172 (≠ S185) mutation to A: No zinc bound and no enzyme activity.
C175 (vs. gap) mutation to A: No zinc bound and no enzyme activity.
C187 (= C202) mutation to A: No effect.
C231 (≠ A246) mutation to A: No effect.

Sites not aligning to the query:

244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
44% identity, 96% coverage: 9:255/258 of query aligns to 1:232/240 of 1jwbB

query
sites
1jwbB

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G
|
G

A

L

G
|
G

L

L

G

G

A

C

P

A

V

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D
|
D

D
x
F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

A

S

T

D

E

A

D

T

I

V

G

G

R

Q

A

P

K
|
K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

I

T

-

P

T

V

H

N

A

A

L
|
L

R

L

L

D

G

D

A

A

E

E

N

L

A

A

R

-

E

A

L

L

F

I

D

A

A

E

Y

H

D

D

L

L

V

V

V

L

D

D

G
x
C

S
x
T

D
|
D

N
|
N

F

V

D

A

T
x
V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A

C

E

F

A

A

A

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

E

-

T

T

D

Y

A

Q

D

D

G

G

H

E

P

-

N

-

P

P

S
x
C

Y

Y

R

R

-

C

-

L

-

S

D

R

L

L

F

F

P

-

E

-

P

-

G

-

A

-

V

-

P

-

S

-

C

-

A

G

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A

C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

active site: R13 (= R21), D129 (= D137)
binding adenosine monophosphate: G37 (= G45), G39 (= G47), G40 (= G48), D61 (= D69), F62 (≠ D70), K85 (= K93), L108 (= L115), C127 (≠ G135), T128 (≠ S136), D129 (= D137), N130 (= N138), V133 (≠ T141)
binding zinc ion: C171 (≠ S185)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
44% identity, 96% coverage: 9:255/258 of query aligns to 1:232/240 of 1jw9B

query
sites
1jw9B

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G

G

L

L

G

G

A

C

P

A

V

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D

D

D

F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

A

S

T

D

E

A

D

T

I

V

G

G

R

Q

A

P

K

K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

I

T

-

P

T

V

H

N

A

A

L

L

R

L

L

D

G

D

A

A

E

E

N

L

A

A

R

-

E

A

L

L

F

I

D

A

A

E

Y

H

D

D

L

L

V

V

V

L

D

D

G

C

S

T

D
|
D

N

N

F

V

D

A

T

V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A

C

E

F

A

A

A

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

E

-

T

T

D

Y

A

Q

D

D

G

G

H

E

P

-

N

-

P

P

S
x
C

Y

Y

R

R

-

C

-

L

-

S

D

R

L

L

F

F

P

-

E

-

P

-

G

-

A

-

V

-

P

-

S

-

C

-

A

G

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A

C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

active site: R13 (= R21), D129 (= D137)
binding zinc ion: C171 (≠ S185)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
42% identity, 96% coverage: 9:255/258 of query aligns to 1:217/217 of 1jwaB

query
sites
1jwaB

A

A

T

E

L

L

A

S

P

D

S

Q

E

E

I

M

A

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

P

R

E

G

I

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

R

R

V

V

L

L

V

I

I

V

G

G

A

L

G
|
G

L

L

G

G

A

C

P

A

V

A

L

S

Q

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

T

N

L

L

G

T

I

L

V

L

D
|
D

D
x
F

D

D

V

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

A

S

T

D

E

A

D

T

I

V

G

G

R

Q

A

P

K
|
K

V

V

E

E

S

S

A

A

A

R

A

D

A

A

I

L

D

T

A

R

L

I

N

N

P

P

H

H

V

I

K

A

V

I

I

T

-

P

T

V

H

N

A

A

L
|
L

R

L

L

D

G

D

A

A

E

E

N

L

A

A

R

-

E

A

L

L

F

I

D

A

A

E

Y

H

D

D

L

L

V

V

V

L

D

D

G

C

S

T

D
|
D

N
|
N

F

V

D

A

T
x
V

R

R

Y

N

L

Q

A

L

A

N

D

A

T

G

A

C

E

F

A

A

A

K

V

V

A

P

L

L

V

V

T

S

G

G

A

A

V

A

G

I

R

R

F

M

D

E

G

G

S

Q

V

I

T

T

V

V

L

F

K

-

P

-

Y

-

E

-

T

-

D

-

A

-

D

-

G

-

H

-

P

-

N

-

P

-

S

T

Y

Y

R

-

D

-

L

-

F

-

P

-

E

-

P

-

G

-

A

-

V

-

P

-

S

-

C

-

A

-

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

T

I

G

G

V

V

I

I

G

G

T

S

L

L

Q

Q

A

A

M

M

E

E

A

A

I

I

K

K

L

M

V

L

T

A

G

G

I

Y

G

G

E

K

P

P

L

A

I

S

G

G

R

K

L

I

L

V

L

M

Y

Y

D

D

A

A

L

M

A

T

A

C

Q

Q

F

F

N

R

T

E

I

M

R

K

Y

L

R

M

R

R

active site: R13 (= R21), D129 (= D137)
binding adenosine-5'-triphosphate: G39 (= G47), G40 (= G48), D61 (= D69), F62 (≠ D70), R72 (= R80), K85 (= K93), L108 (= L115), D129 (= D137), N130 (= N138), V133 (≠ T141)

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
39% identity, 86% coverage: 6:227/258 of query aligns to 1:213/423 of 6yubA

query
sites
6yubA

T

S

T

T

G

Q

A

K

T

S

L

L

A

S

P

K

S

E

E

E

I

I

A

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

P

P

E

G

I

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

K

R

L

L

K

M

A

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

V

A

L

A

Q

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

G

G

I

I

V

V

D

D

D

G

D

D

V

S

V

V

S

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

A

A

T

T

E

K

D

R

I

V

G

G

R

M

A

L

K

K

V

V

E

D

S

S

A

L

A

I

A

T

A

H

I

L

D

I

A

E

L

I

N

N

P

P

H

L

V

P

K

V

V

Y

I

V

T

P

H

Y

A

R

L

F

R

D

L

L

G

T

A

P

E

Q

N

N

A

A

R

A

E

Q

L

I

F

I

D

K

A

P

Y

W

D

D

L

V

V

I

V

L

D

D

G

C

S

T

D

D

N

N

F

P

D

A

T

T

R

R

Y

Y

L

L

A

I

A

S

D

D

T

V

A

C

E

V

A

L

A

G

V

K

A

P

L

L

V

V

T

S

G

A

A

A

V

S

G

V

R

Q

F

K

D

S

G

G

S

Q

V

L

T

I

V

V

L

L

K

N

P

C

Y

P

E

P

T

T

D

P

-

Q

-

G

-

V

A

V

D

N

G

K

H

K

P

A

N

A

P

P

S
x
C

Y

Y

R

R

D
x
C

L

C

F

F

P

K

E

K

P

P

P

-

G

-

A

-

V

-

P

-

S

-

C

-

A

-

E

-

A

-

G

G

V

I

L

M

G

G

A

P

L

V

T

V

G

G

V

M

I

M

G

G

T

V

L

A

Q

Q

A

A

M

G

E

E

A

A

I

I

K

K

L

I

V

L

binding zinc ion: C183 (≠ S185), C186 (≠ D188)

Sites not aligning to the query:

binding zinc ion: 266, 269

1zfnA Structural analysis of escherichia coli thif (see paper)
41% identity, 93% coverage: 15:255/258 of query aligns to 5:236/244 of 1zfnA

query
sites
1zfnA

E

D

I

F

A

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V

L

L

L

P

D

E

D

I

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

R

Q

V

V

L

L

V

I

I
|
I

G
|
G

A

L

G
|
G

L

L

G

G

A

T

P

P

V

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

V

A

D
|
D

D

D

V

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

V

I

I

L

H

F

A

T

T

T

E

E

D

D

I

I

G

D

R

R

A

P

K
|
K

V

S

E

Q

S

V

A

S

A

Q

A

R

I

L

D

T

A

Q

L

L

N

N

P

P

H

D

V

I

K

Q

V

L

I

T

T

A

H

L

A

Q

L

Q

R

R

L

L

G

T

A

G

E

E

N

A

A

L

R

K

E

D

L

A

F

V

D

A

A

R

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

S
x
T

D
|
D

N

N

F

M

D

A

T
|
T

R

R

Y

Q

L

E

A

I

A

N

D

A

T

A

A

C

E

V

A

A

A

L

A

N

V

T

A

P

L

L

V

I

T

T

G

A

A

S

V

A

G

V

R

G

F

F

D

G

G

G

S

Q

V

L

T

M

V

V

L

L

K

T

-

P

P

P

Y

W

E

E

T

-

D

-

A

-

D

-

G

-

H

-

P

-

N

Q

P

G

S
x
C

Y

Y

R

R

D
x
C

L

L

F

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

A

R

V

-

P

-

S

N

C

C

A

R

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

T

T

L

L

Q

Q

A

A

M

L

E

E

A

A

I

I

K

K

L

L

V

L

T

S

G

G

I

I

G

E

E

T

P

P

L

-

I

A

G

G

R

E

L

L

L

R

L

L

Y

F

D

D

A

G

L

K

A

S

Q

Q

F

W

N

R

T

S

I

L

R

A

Y

L

R

R

active site: R11 (= R21), D127 (= D137)
binding adenosine-5'-triphosphate: I34 (= I44), G35 (= G45), G37 (= G47), G38 (= G48), D59 (= D69), R70 (= R80), Q71 (= Q81), K83 (= K93), T126 (≠ S136), D127 (= D137), T131 (= T141)
binding zinc ion: C169 (≠ S185), C172 (≠ D188)

Sites not aligning to the query:

binding zinc ion: 240, 243

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
41% identity, 93% coverage: 15:255/258 of query aligns to 5:236/251 of P30138

query
sites
P30138

E

D

I

F

A

M

R

R

Y

Y

A

S

R

R

H

Q

I

I

V

L

L

L

P

D

E

D

I

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

V

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

V

A

D

D

V

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

A

T

T

T

E

E

D

D

I

I

G

D

R

R

A

P

K

K

V

S

E

Q

S

V

A

S

A

Q

A

R

I

L

D

T

A

Q

L

L

N

N

P

P

H

D

V

I

K

Q

V

L

I

T

T

A

H

L

A

Q

L

Q

R

R

L

L

G

T

A

G

E

E

N

A

A

L

R

K

E

D

L

A

F

V

D

A

A

R

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

S

T

D

D

N

N

F

M

D

A

T

T

R

R

Y

Q

L

E

A

I

A

N

D

A

T

A

A

C

E

V

A

A

A

L

A

N

V

T

A

P

L

L

V

I

T

T

G

A

A

S

V

A

G

V

R

G

F

F

D

G

G

G

S

Q

V

L

T

M

V

V

L

L

K

T

-

P

P

P

Y

W

E

E

T

-

D

-

A

-

D

-

G

-

H

-

P

-

N

Q

P

G

S
x
C

Y

Y

R

R

D
x
C

L

L

F
x
W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

A

R

V

-

P

-

S

N

C
|
C

A

R

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

T

T

L

L

Q

Q

A

A

M

L

E

E

A

A

I

I

K

K

L

L

V

L

T

S

G

G

I

I

G

E

E

T

P

P

L

-

I

A

G

G

R

E

L

L

L

R

L

L

Y

F

D

D

A

G

L

K

A

S

Q

Q

F

W

N

R

T

S

I

L

R

A

Y

L

R

R

C169 (≠ S185) binding Zn(2+)
C172 (≠ D188) binding Zn(2+)
W174 (≠ F190) mutation to A: No adenylation of ThiS.
C184 (= C202) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.

Sites not aligning to the query:

240 binding Zn(2+)
243 binding Zn(2+)

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
38% identity, 86% coverage: 6:227/258 of query aligns to 2:212/289 of 6yubB

query
sites
6yubB

T

S

T

T

G

Q

A

K

T

S

L

L

A

S

P

K

S

E

E

E

I

I

A

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

P

P

E

G

I

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

K

R

L

L

K

M

A

N

A

A

R

K

V

V

L

L

V

I

I

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

V

A

L

A

Q

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

I

G

G

I

I

V

V

D

D

D

G

D

D

V

S

V

V

S

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

A

A

T

T

E

K

D

R

I

V

G

G

R

M

A

L

K

K

V

V

E

D

S

S

A

L

A

I

A

T

A

H

I

L

D

I

A

E

L

I

N

N

P

P

H

L

V

P

K

V

V

Y

I

V

T

P

H

Y

A

R

L

F

R

D

L

L

G

T

A

P

E

Q

N

N

A

A

R

A

E

Q

L

I

F

I

D

K

A

P

Y

W

D

D

L

V

V

I

V

L

D

D

G

C

S

T

D

D

N

N

F

P

D

A

T

T

R

R

Y

Y

L

L

A

I

A

S

D

D

T

V

A

C

E

V

A

L

A

G

V

K

A

P

L

L

V

V

T

S

G

A

A

A

V

S

G

V

R

Q

F

K

D

S

G

G

S

Q

V

L

T

I

V

V

L

L

K

N

P

C

Y

P

E

P

T

T

D

P

-

Q

-

G

-

V

A

V

D

N

G

K

H

K

P

A

N

A

P

P

S
x
C

Y

Y

R

R

D

-

L

-

F

-

P

-

E

-

P

-

G

-

A

-

V

-

P

-

S

-

C
|
C

A

C

E

F

A

K

G

G

V

I

L

M

G

G

A

P

L

V

T

V

G

G

V

M

I

M

G

G

T

V

L

A

Q

Q

A

A

M

G

E

E

A

A

I

I

K

K

L

I

V

L

binding zinc ion: C184 (≠ S185), C187 (= C202)

Sites not aligning to the query:

binding zinc ion: 265

1zud3 Structure of this-thif protein complex (see paper)
40% identity, 93% coverage: 15:255/258 of query aligns to 5:231/240 of 1zud3

query
sites
1zud3

E

D

I

F

A

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V
x
L

L

L

P
x
D

E

D

I

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

R

Q

V

V

L

L

V

I

I

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

V

A

L

A

Q

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

T

T

L

L

G

V

I

L

V

A

D

D

V

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

A

T

T

T

E

E

D

D

I

I

G

D

R

R

A

P

K

K

V

S

E

Q

S

V

A

S

A

Q

A

R

I

L

D

T

A

Q

L

L

N

N

P

P

H

D

V

I

K

Q

V

L

I

T

T

A

H

L

A

Q

L

Q

R

R

L

L

G

T

A

G

E

E

N

A

A

L

R

K

E

D

L

A

F

V

D

A

A

R

Y

A

D

D

L

V

V

V

V

L

D

D

G

C

S

T

D
|
D

N

N

F

M

D

A

T

T

R

R

Y

Q

L

E

A

I

A

N

D

A

T

A

A

C

E

V

A

A

A

L

A

N

V

T

A

P

L

L

V

I

T

T

G

A

A

S

V

A

G

V

R

G

F

F

D

G

G

G

S

Q

V

L

T

M

V

V

L

L

K

T

-

P

P

P

Y

W

E

E

T

-

D

-

A

-

D

-

G

-

H

-

P

-

N

Q

P

G

S
x
C

Y

Y

R

R

D

C

L

L

F

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

T

A

A

V

G

P

V

S

V

C

-

A

-

E

-

A

-

G

-

V

-

L

-

G

G

A

P

L

V

T

V

G

G

V

V

I

M

G

G

T

T

L

L

Q

Q

A

A

M

L

E

E

A

A

I

I

K

K

L

L

V

L

T

S

G

G

I

I

G

E

E

T

P

P

L

-

I

A

G

G

R

E

L

L

L

R

L

L

Y

F

D

D

A

G

L

K

A

S

Q

Q

F

W

N

R

T

S

I

L

R

A

Y

L

R

R

active site: R11 (= R21), D127 (= D137)
binding calcium ion: L14 (≠ V24), D16 (≠ P26)
binding zinc ion: C169 (≠ S185)

Sites not aligning to the query:

binding zinc ion: 235, 238

P22314 Ubiquitin-like modifier-activating enzyme 1; Protein A1S9; Ubiquitin-activating enzyme E1; EC 6.2.1.45 from Homo sapiens (Human) (see 2 papers)
30% identity, 67% coverage: 29:202/258 of query aligns to 459:632/1058 of P22314

query
sites
P22314

G

G

G

S

P

D

G

L

Q

Q

Q

E

K

K

L

L

K

G

A

K

A

Q

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K

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Y

L

F

V

L

I

V

G

G

A

A

G

G

G

A

L

I

G

G

A

C

P

E

V

L

L

L

Q

K

Y

N

L

F

A

A

A

M

A

I

G

G

V

L

G

G

T

C

-

G

-

E

-

G

-

E

L

I

G

I

I

V

V

T

D

D

D

M

D

D

V

T

V

I

S

E

L

K

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

A

R

T

P

E

W

D

D

I

V

G

T

R

K

A

L

K

K

V

S

E

D

S

T

A

A

I

V

D

R

A

Q

L

M

N

N

P

P

H

H

V

I

K

R

V

V

I

T

T

S

H

H

A

Q

L

N

R

R

L

V

G

G

A

P

E

D

N

T

A

E

R

R

-

I

-

Y

-

D

E

D

L

F

F

F

D

Q

A

N

Y

L

D

D

L

G

V

V

V

A

D

N

G

A

S

L

D

D

N

N

F

V

D

D

T

A

R

R

-

M

Y

Y

L

M

A

D

A

R

D

R

T

C

A

V

E

Y

A

Y

A

R

A

K

V

P

A

L

L

L

V

E

T

S

G

G

A

T

V

L

G

G

R

T

F

-

D

K

G

G

S

N

V

V

T

Q

V

V

L

V

K

I

P

P

Y

F

E

L

T

T

D

E

A

-

D

-

G

-

H

-

P

-

N

-

P

-

S

S

Y

Y

R

S

D

S

L

-

F

-

P

S

E

Q

P

D

P

P

P

E

G

K

A

S

V

I

P

P

S

I

C

C

Sites not aligning to the query:

1:66 mutation Missing: Localizes to the cytoplasm.
4 modified: Phosphoserine; S→A: Reduces phosphorylation.
8:11 KKRR→AAAA: Loss of nuclear localization and a 90-95% decrease in the phosphorylation.
41 M → L: in VEXAS; somatic mutation; the underlying nucleotide substitution affects normal alternative translation initiation and leads to aberrant initiation from M-67 to produce a shorter protein with strongly reduced enzymatic activity; M → T: in VEXAS; somatic mutation; the underlying nucleotide substitution affects normal alternative translation initiation and leads to aberrant initiation from M-67 to produce a shorter protein with strongly reduced enzymatic activity; M → V: in VEXAS; somatic mutation; does not affect ubiquitin activating enzyme activity; does not affect subcellular localization; the underlying nucleotide substitution affects normal alternative translation initiation and leads to aberrant initiation from M-67 to produce a shorter protein with strongly reduced enzymatic activity; M→A: Localizes to the nucleus; when associated with A-67.
67 M→A: Localizes to the nucleus; when associated with A-41. Localizes to the cytoplasm; when associated with 1-M--G-40 del.

O42939 Ubiquitin-activating enzyme E1-like; Pmt3-activating enzyme subunit 2 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
30% identity, 66% coverage: 34:203/258 of query aligns to 21:182/628 of O42939

query
sites
O42939

Q

R

K

N

L

F

K

K

A

S

A

A

R

K

V

V

L

L

V

L

I

V

G

G

A

A

G

G

L

I

G

G

A

C

P

E

V

L

L

L

Q

K

Y

N

L

L

A

L

A

M

A

S

G

G

V

V

G

K

T

E

L

V

G

H

I

I

V

I

D

D

D

L

D

D

V

T

V

I

S

D

L

L

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

A

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K

D

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K

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A

P

K

K

V

A

E

I

S

V

A

A

A

K

A

T

I

A

D

S

A

S

L

F

N

N

P

P

H

N

V

V

K

K

V

L

I

E

T

A

H

Y

A

H

L

A

R

N

L

I

G

K

A

E

E

D

N

R

A

F

R

N

-

V

E

A

L

W

F

F

D

R

A

Q

Y

F

D

D

L

L

V

V

V

F

D

N

G

A

S

L

D

D

N

N

F

L

D

D

T

A

R

R

Y

R

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H

A

V

A

N

D

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T

Q

A

C

E

L

A

L

A

A

A

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L

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G

S

A

G

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T

G

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F

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Q

V

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Q

V

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L

I

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I

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Y

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T

D

E

A

C

-

Y

D

D

G

C

H

N

P

P

N

K

P

-

S

-

Y

-

R

-

D

-

L

-

F

-

P

-

E

-

P

-

P

E

P

P

G

K

A

T

V

Y

P

P

S

V

C

C

A

T

Sites not aligning to the query:

559 modified: Phosphoserine
563 modified: Phosphoserine

3kydB Human sumo e1~sumo1-amp tetrahedral intermediate mimic (see paper)
28% identity, 79% coverage: 40:244/258 of query aligns to 16:227/477 of 3kydB

query
sites
3kydB

R

R

V

V

L

L

V

V

I

V

G

G

A

A

G

G

L
x
I

G

G

A

C

P

E

V

L

L

L

Q

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

S

T

H

L

I

G

D

I

L

V

I

D
|
D

D
x
L

D

D

V

T

V

I

S

D

L

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

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I

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F

A

Q

T

K

E

K

D

H

I

V

G

G

R

R

A

S

K
|
K

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A

E

Q

S

V

A

A

A

K

A

E

A

S

I

V

D

L

A

Q

L

F

N

Y

P

P

H

K

V

A

K

N

V

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I

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T

A

-

Y

H

H

A

D

L
x
S

R
x
I

L

M

G

N

A

P

E

D

N

Y

A

N

R

V

E

E

L

F

F

F

D

R

A

Q

Y

F

D

I

L

L

V

V

V

M

D

N

G

A

S
x
L

D
|
D

N
|
N

F

R

D

A

T

A

R

R

Y

N

L

H

A

V

A

N

D

R

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M

A

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L

A

A

A

D

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V

A

P

L

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D

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T

V

T

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I

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K

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K

Y

G

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V

T

T

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E

A
x
C

-

Y

D

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G
x
C

H

H

P

P

N

K

P

P

S

T

Y

Q

R

R

D

-

L

T

F

F

P

P

-

G

-
x
C

-
x
T

-

I

-
x
R

-

N

E

T

P

P

P

S

P

E

P

P

G

I

A

H

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C

P

I

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V

C

W

A

A

E

K

A

Y

G

L

V

F

L

N

G

Q

A

L

L

F

T

T

G

K

V

E

I

W

G

A

T

K

L

S

Q

T

A

G

M

Y

E

D

A

P

I

V

K

K

L

L

V

F

T

T

G

K

I

L

G

F

E

K

P

D

L

D

I

I

G

R

R

Y

L

L

L

T

Y

M

D

D

A

K

L

L

active site: D114 (= D137), C170 (vs. gap), T171 (vs. gap), R173 (vs. gap)
binding 5'-{[(3-aminopropyl)sulfonyl]amino}-5'-deoxyadenosine: I25 (≠ L49), D45 (= D69), L46 (≠ D70), K69 (= K93), S92 (≠ L115), I93 (≠ R116), L113 (≠ S136), D114 (= D137), N115 (= N138), C170 (vs. gap), R173 (vs. gap)
binding zinc ion: C155 (≠ A178), C158 (≠ G180)

Sites not aligning to the query:

binding zinc ion: 370, 373

6dc6C Crystal structure of human ubiquitin activating enzyme e1 (uba1) in complex with ubiquitin (see paper)
30% identity, 67% coverage: 29:200/258 of query aligns to 411:582/996 of 6dc6C

query
sites
6dc6C

G

G

G

S

P

D

G

L

Q

Q

Q

E

K

K

L

L

K

G

A

K

A

Q

R

K

V

Y

L

F

V

L

I

V

G

G

A

A

G

G

G

A

L

I

G

G

A

C

P

E

V

L

L

L

Q

K

Y

N

L

F

A

A

A

M

A

I

G

G

V

L

G

G

T

C

-

G

-

E

-

G

-

E

L

I

G

I

I

V

V

T

D

D

D

M

D
|
D

V

T

V

I

S
x
E

L

K

S

S

N
|
N

L

L

Q

N

R
|
R

Q

Q

V

F

I

L

H

F

A

R

T

P

E

W

D

D

I

V

G

T

R

K

A

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K
|
K

V

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A

A

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V

D

R

A

Q

L

M

N

N

P

P

H

H

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V

I

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T

S

H

H

A

Q

L

N

R

R

L

V

G

G

A

P

E

D

N

T

A

E

R

R

-

I

-

Y

-

D

E

D

L

F

F

F

D

Q

A

N

Y

L

D

D

L

G

V

V

V

A

D

N

G

A

S

L

D
|
D

N

N

F

V

D

D

T

A

R

R

-

M

Y

Y

L

M

A

D

A

R

D

R

T

C

A

V

E

Y

A

Y

A

R

A

K

V

P

A

L

L

L

V

E

T

S

G

G

A

T

V

L

G

G

R

T

F

-

D

K

G

G

S

N

V

V

T

Q

V

V

L

V

K

I

P

P

Y

F

E

L

T

T

D

E

A

-

D

-

G

-

H

-

P

-

N

-

P

-

S

S

Y

Y

R

S

D

S

L

-

F

-

P

S

E

Q

P

D

P

P

P

E

G

K

A

S

V

I

P

P

active site: R467 (= R80), D528 (= D137)
binding magnesium ion: D458 (= D71), E461 (≠ S74)
binding pyrophosphate 2-: N464 (= N77), K480 (= K93)

Sites not aligning to the query:

active site: 9, 584, 585, 587
binding pyrophosphate 2-: 9

Q7SXG4 SUMO-activating enzyme subunit 2; Ubiquitin-like 1-activating enzyme E1B; Ubiquitin-like modifier-activating enzyme 2; EC 2.3.2.- from Danio rerio (Zebrafish) (Brachydanio rerio) (see paper)
28% identity, 65% coverage: 25:191/258 of query aligns to 1:172/650 of Q7SXG4

query
sites
Q7SXG4

L

M

P

A

E

E

I

L

G

V

G

G

P

P

G

L

Q

R

Q

K

K

Q

L

L

-

A

-

D

-

S

-

L

K

S

A

S

A

C

R

R

V

V

L

L

V

V

I

V

G

G

A

A

G

G

L

I

G

G

A

C

P

E

V

L

L

L

Q

K

Y

N

L

L

A

V

A

L

A

T

G

G

V

F

G

K

T

N

L

I

G

E

I

V

V

I

D

D

D

L

D

D

V

T

V

I

S

D

L

V

S

S

N

N

L

L

Q

N

R

R

Q

Q

V

F

I

L

H

F

A

Q

T

K

E

K

D

H

I

V

G

G

R

K

A

S

K

K

V

A

E

Q

S

V

A

A

A

K

A

E

A

S

I

V

D

L

A

R

L

F

N

C

P

P

H

S

V

A

K

N

V

I

I

T

T

A

-

Y

H

H

A

D

L

S

R

I

L

M

G

N

A

P

E

D

N

Y

A

N

R

V

E

E

L

F

F

F

D

R

A

N

Y

F

D

Q

L

L

V

V

V

M

D

N

G

A

S

L

D

D

N

N

F

R

D

A

T

A

R

R

Y

N

L

H

A

V

A

N

D

R

T

M

A

C

E

L

A

A

A

D

V

I

A

P

L

L

V

I

T

E

G

S

A

G

V

T

G

A

R

G

F

Y

D

L

G

G

S

Q

V

V

T

T

V

V

L

I

K

K

P

K

Y

G

E

Q

T

T

D

E

A

C

-

Y

D

E

G

C

H

Q

P

P

N

K

P

P

S

T

Y

Q

R

K

D

-

L

T

F

F

P

P

Sites not aligning to the query:

599 modified: Phosphoserine

Query Sequence

>WP_012067598.1 NCBI__GCF_000017145.1:WP_012067598.1
METHMTTGATLAPSEIARYARHIVLPEIGGPGQQKLKAARVLVIGAGGLGAPVLQYLAAA
GVGTLGIVDDDVVSLSNLQRQVIHATEDIGRAKVESAAAAIDALNPHVKVITHALRLGAE
NARELFDAYDLVVDGSDNFDTRYLAADTAEAAAVALVTGAVGRFDGSVTVLKPYETDADG
HPNPSYRDLFPEPPPPGAVPSCAEAGVLGALTGVIGTLQAMEAIKLVTGIGEPLIGRLLL
YDALAAQFNTIRYRRGKR

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory