SitesBLAST
Comparing WP_012101318.1 NCBI__GCF_000016505.1:WP_012101318.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
3du4A Crystal structure of 7-keto-8-aminopelargonic acid bound 7,8- diaminopelargonic acid synthase in bacillus subtilis (see paper)
41% identity, 98% coverage: 2:444/451 of query aligns to 3:442/448 of 3du4A
- active site: F17 (≠ C16), Y146 (= Y146), E217 (= E218), D251 (= D252), A254 (= A255), K280 (= K281), A417 (≠ Y419)
- binding 7-keto-8-aminopelargonic acid: L82 (≠ A82), Y146 (= Y146), G315 (≠ S316), S317 (≠ T318), R410 (= R412)
- binding pyridoxal-5'-phosphate: S112 (≠ N112), G113 (= G113), A114 (≠ S114), Y146 (= Y146), H147 (= H147), E217 (= E218), D251 (= D252), V253 (≠ I254), A254 (= A255), K280 (= K281), H316 (= H317), S317 (≠ T318)
P53555 L-Lysine--8-amino-7-oxononanoate transaminase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; L-Lysine--8-amino-7-oxononanoate aminotransferase; EC 2.6.1.105 from Bacillus subtilis (strain 168) (see paper)
41% identity, 98% coverage: 2:444/451 of query aligns to 3:442/448 of P53555
- GA 113:114 (≠ GS 113:114) binding pyridoxal 5'-phosphate
- Y146 (= Y146) binding substrate
- K280 (= K281) modified: N6-(pyridoxal phosphate)lysine
- G315 (≠ S316) binding substrate
- HS 316:317 (≠ HT 317:318) binding pyridoxal 5'-phosphate
- R410 (= R412) binding substrate
6zhkA Crystal structure of adenosylmethionine-8-amino-7-oxononanoate aminotransferase from methanocaldococcus jannaschii dsm 2661
42% identity, 97% coverage: 6:444/451 of query aligns to 7:435/438 of 6zhkA
6wnnA Bacillus subtilis bioa in complex with amino donor l-lys
39% identity, 98% coverage: 3:444/451 of query aligns to 1:414/420 of 6wnnA
- active site: F14 (≠ C16), Y143 (= Y146), D223 (= D252), K252 (= K281)
- binding (2S)-2-amino-6-[[3-hydroxy-2-methyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]hexanoic acid: F14 (≠ C16), W51 (= W54), S109 (≠ N112), G110 (= G113), A111 (≠ S114), Y143 (= Y146), H144 (= H147), D223 (= D252), V225 (≠ I254), K252 (= K281), R382 (= R412)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: H288 (= H317), S289 (≠ T318)
3dodA Crystal structure of plp bound 7,8-diaminopelargonic acid synthase in bacillus subtilis (see paper)
39% identity, 98% coverage: 2:444/451 of query aligns to 1:411/417 of 3dodA
- active site: F15 (≠ C16), E186 (= E218), D220 (= D252), A223 (= A255), K249 (= K281), A386 (≠ Y419)
- binding pyridoxal-5'-phosphate: W52 (= W54), S110 (≠ N112), G111 (= G113), A112 (≠ S114), D220 (= D252), V222 (≠ I254), K249 (= K281), H285 (= H317), S286 (≠ T318)
6erkA Crystal structure of diaminopelargonic acid aminotransferase from psychrobacter cryohalolentis (see paper)
33% identity, 99% coverage: 2:446/451 of query aligns to 7:417/420 of 6erkA
P12995 Adenosylmethionine-8-amino-7-oxononanoate aminotransferase; 7,8-diamino-pelargonic acid aminotransferase; DAPA AT; DAPA aminotransferase; 7,8-diaminononanoate synthase; DANS; Diaminopelargonic acid synthase; EC 2.6.1.62 from Escherichia coli (strain K12) (see 4 papers)
36% identity, 99% coverage: 2:448/451 of query aligns to 3:429/429 of P12995
- Y17 (≠ C16) mutation to F: Severely reduces the aminotransferase activity.
- W52 (= W53) binding substrate
- GS 112:113 (= GS 113:114) binding pyridoxal 5'-phosphate
- Y144 (= Y146) mutation to F: Severely reduces the aminotransferase activity.
- D147 (≠ E149) mutation to N: Loss of aminotransferase activity.
- D245 (= D252) binding pyridoxal 5'-phosphate
- R253 (= R260) mutation to A: Has only a small effect on the rate of reaction with DAPA.; mutation to K: Increases aminotransferase activity toward SAM.; mutation to M: Loss of aminotransferase activity.; mutation to Q: Increases aminotransferase activity toward SAM.
- K274 (= K281) binding substrate; modified: N6-(pyridoxal phosphate)lysine
- G307 (≠ S316) binding substrate
- PT 308:309 (≠ HT 317:318) binding pyridoxal 5'-phosphate
- R391 (= R412) binding substrate; mutation to A: Reduces aminotransferase activity.
6ed7A Crystal structure of 7,8-diaminopelargonic acid synthase bound to inhibitor mac13772
36% identity, 99% coverage: 2:448/451 of query aligns to 3:429/429 of 6ed7A
- active site: Y17 (≠ C16), Y144 (= Y146), D245 (= D252), K274 (= K281)
- binding 2-[(2-nitrophenyl)sulfanyl]acetohydrazide: Y17 (≠ C16), W52 (= W53), W52 (= W53), Y144 (= Y146), D147 (≠ E149), A217 (= A224), K274 (= K281), R391 (= R412), F393 (≠ I414), F393 (≠ I414)
- binding pyridoxal-5'-phosphate: G112 (= G113), S113 (= S114), Y144 (= Y146), H145 (= H147), D245 (= D252), I247 (= I254), K274 (= K281)
1dtyA Crystal structure of adenosylmethionine-8-amino-7-oxonanoate aminotransferase with pyridoxal phosphate cofactor.
36% identity, 99% coverage: 2:448/451 of query aligns to 3:429/429 of 1dtyA
- active site: Y17 (≠ C16), Y144 (= Y146), E211 (= E218), D245 (= D252), A248 (= A255), K274 (= K281), Y398 (= Y419)
- binding pyridoxal-5'-phosphate: G112 (= G113), S113 (= S114), Y144 (= Y146), H145 (= H147), D245 (= D252), I247 (= I254), K274 (= K281)
1mlzA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the trans-isomer of amiclenomycin. (see paper)
36% identity, 99% coverage: 2:446/451 of query aligns to 3:426/427 of 1mlzA
- active site: Y17 (≠ C16), Y144 (= Y146), E210 (= E218), D244 (= D252), A247 (= A255), K273 (= K281), Y397 (= Y419)
- binding pyridoxal-5'-phosphate: G112 (= G113), S113 (= S114), Y144 (= Y146), H145 (= H147), D244 (= D252), I246 (= I254), K273 (= K281), P307 (≠ H317), T308 (= T318)
- binding trans-amiclenomycin: W52 (= W53), W53 (= W54), Y144 (= Y146), K273 (= K281), R390 (= R412), F392 (≠ I414)
1mlyA Crystal structure of 7,8-diaminopelargonic acid synthase in complex with the cis isomer of amiclenomycin (see paper)
36% identity, 99% coverage: 2:446/451 of query aligns to 3:426/427 of 1mlyA
- active site: Y17 (≠ C16), Y144 (= Y146), E210 (= E218), D244 (= D252), A247 (= A255), K273 (= K281), Y397 (= Y419)
- binding cis-amiclenomycin: W52 (= W53), W53 (= W54), K273 (= K281), R390 (= R412), F392 (≠ I414)
- binding pyridoxal-5'-phosphate: G112 (= G113), S113 (= S114), Y144 (= Y146), H145 (= H147), D244 (= D252), I246 (= I254), K273 (= K281), P307 (≠ H317), T308 (= T318)
1qj3A Crystal structure of 7,8-diaminopelargonic acid synthase in complex with 7-keto-8-aminopelargonic acid (see paper)
35% identity, 99% coverage: 2:446/451 of query aligns to 3:415/416 of 1qj3A
- active site: Y17 (≠ C16), Y144 (= Y146), E201 (= E218), D235 (= D252), A238 (= A255), K264 (= K281), Y386 (= Y419)
- binding 7-keto-8-aminopelargonic acid: Y17 (≠ C16), W52 (= W53), Y144 (= Y146), K264 (= K281), R379 (= R412), F381 (≠ I414)
- binding pyridoxal-5'-phosphate: G112 (= G113), S113 (= S114), Y144 (= Y146), H145 (= H147), G146 (= G148), D235 (= D252), I237 (= I254), A238 (= A255), K264 (= K281)
6g4fA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with pmp (see paper)
31% identity, 97% coverage: 6:444/451 of query aligns to 5:444/451 of 6g4fA
- active site: Y15 (≠ C16), Y146 (= Y146), D253 (= D252), K282 (= K281)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S112 (≠ N112), G113 (= G113), S114 (= S114), Y146 (= Y146), H147 (= H147), E220 (= E218), D253 (= D252), V255 (≠ I254), I256 (≠ A255), K282 (= K281)
6g4eA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp and 6-aminohexanoate (6-aca) (see paper)
31% identity, 97% coverage: 6:444/451 of query aligns to 5:444/451 of 6g4eA
- active site: Y15 (≠ C16), Y146 (= Y146), D253 (= D252), K282 (= K281)
- binding 6-aminohexanoic acid: K282 (= K281), R412 (= R412), M414 (≠ I414)
- binding pyridoxal-5'-phosphate: S112 (≠ N112), G113 (= G113), S114 (= S114), Y146 (= Y146), H147 (= H147), E220 (= E218), D253 (= D252), V255 (≠ I254), I256 (≠ A255), K282 (= K281)
6g4dB Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp (see paper)
31% identity, 97% coverage: 6:444/451 of query aligns to 5:444/453 of 6g4dB
- active site: Y15 (≠ C16), Y146 (= Y146), D253 (= D252), K282 (= K281)
- binding pyridoxal-5'-phosphate: S112 (≠ N112), G113 (= G113), S114 (= S114), Y146 (= Y146), H147 (= H147), E220 (= E218), D253 (= D252), V255 (≠ I254), I256 (≠ A255), K282 (= K281)
6s54A Transaminase from pseudomonas fluorescens (see paper)
32% identity, 97% coverage: 11:449/451 of query aligns to 15:451/453 of 6s54A
- active site: M20 (≠ C16), Y152 (= Y146), D258 (= D252), K287 (= K281)
- binding pyridoxal-5'-phosphate: G119 (= G113), S120 (= S114), Y152 (= Y146), H153 (= H147), G154 (= G148), E225 (= E218), D258 (= D252), V260 (≠ I254), I261 (≠ A255), K287 (= K281)
4cxqA Mycobaterium tuberculosis transaminase bioa complexed with substrate kapa (see paper)
33% identity, 95% coverage: 5:432/451 of query aligns to 7:412/427 of 4cxqA
- active site: Y18 (≠ C16), Y149 (= Y146), E212 (= E218), D246 (= D252), A249 (= A255), K275 (= K281), Y399 (= Y419)
- binding 7-keto-8-aminopelargonic acid: W56 (= W53), Y149 (= Y146), G308 (≠ S316), T310 (= T318), R392 (= R412)
- binding pyridoxal-5'-phosphate: G116 (= G113), S117 (= S114), Y149 (= Y146), H150 (= H147), G151 (= G148), E212 (= E218), D246 (= D252), I248 (= I254), K275 (= K281), P309 (≠ H317), T310 (= T318)
5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
34% identity, 95% coverage: 10:436/451 of query aligns to 10:439/449 of 5lh9D
- active site: Y148 (= Y146), D255 (= D252), K284 (= K281), T321 (= T318)
- binding pyridoxal-5'-phosphate: G115 (= G113), S116 (= S114), Y148 (= Y146), H149 (= H147), G150 (= G148), E222 (= E218), D255 (= D252), V257 (≠ I254), K284 (= K281)
5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
34% identity, 95% coverage: 10:436/451 of query aligns to 8:437/447 of 5lhaA
- active site: Y146 (= Y146), D253 (= D252), K282 (= K281), T319 (= T318)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G113 (= G113), S114 (= S114), Y146 (= Y146), H147 (= H147), G148 (= G148), E220 (= E218), D253 (= D252), K282 (= K281), Y318 (≠ H317), T319 (= T318)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
28% identity, 98% coverage: 3:444/451 of query aligns to 7:445/448 of 6io1B
- active site: L20 (≠ C16), Y151 (= Y146), D257 (= D252), K286 (= K281)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (≠ N112), G118 (= G113), A119 (≠ S114), N122 (≠ V117), Y151 (= Y146), H152 (= H147), D257 (= D252), V259 (≠ I254), I260 (≠ A255), K286 (= K281)
Query Sequence
>WP_012101318.1 NCBI__GCF_000016505.1:WP_012101318.1
MNDYVKRDLKYIWHPCGQMKDYEEFNPIVIEKGEGVWLYDIEGNRYLDCVSSWWTNTLGH
SNKRINETIKKQIDNIEHVIFANFSNKPAVDLSEKLVNITPDRLAKVFFSDNGSSAVEIA
LKMSFHYHMQKGNTKKKRFAALSDAYHGETLGALSVCDIDEYNKIYNPLLLDTIRVEGPD
CYRCRYGCTRDNCNAECFADMEKCMEEKHDEISAVIIEPMVQGAAGMKIYSPNYLEKLRK
ICDKYDVHLIADEIAVGFGRAGEMFACNHAQISPDFMCLSKGISAGYMPMSVVMTTDEIY
DCFYGDYNEQKAFLHSHTYAGNATSCAIALESLKILEEDNIIENNRKKGNLIKKLTLEKA
KVSKHIGEVRNIGMITAVEIVKDKFTKKNYPWEMRVGYEIYKIALSKGLLLRPIGNVLYF
IPPYIINEEEIKFMVNKCFQSIEEYFIENKL
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory