SitesBLAST
Comparing WP_012101348.1 NCBI__GCF_000016505.1:WP_012101348.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
8g3hA Structure of cobalamin-dependent methionine synthase (meth) in a resting state (see paper)
31% identity, 99% coverage: 10:798/801 of query aligns to 9:832/841 of 8g3hA
- binding cobalamin: Q328 (≠ E312), T330 (vs. gap), S331 (vs. gap), F675 (≠ Y642), V685 (≠ L652), K693 (= K660), G720 (= G690), V722 (≠ I692), H723 (= H693), D724 (= D694), I725 (= I695), G726 (= G696), V730 (= V700), M767 (≠ L737), S768 (= S738), L770 (= L740), V772 (≠ T742), I795 (≠ V765), L796 (≠ V766), G797 (= G767), G798 (= G768), A799 (= A769), Y818 (= Y784), A819 (= A785), E820 (≠ K786), D821 (= D787)
3bofA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima complexed with zn2+ and homocysteine (see paper)
37% identity, 68% coverage: 10:557/801 of query aligns to 11:554/560 of 3bofA
1q8jA Cobalamin-dependent methionine synthase (1-566) from thermotoga maritima (cd2+, hcy, methyltetrahydrofolate complex) (see paper)
37% identity, 68% coverage: 10:557/801 of query aligns to 11:554/559 of 1q8jA
- binding 5-methyl-5,6,7,8-tetrahydrofolic acid: E320 (= E319), D390 (= D389), N411 (= N410), D473 (= D474), G505 (= G508), N508 (= N511), F511 (= F514), R516 (= R519), I536 (= I539)
P13009 Methionine synthase; 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12-dependent; MS; EC 2.1.1.13 from Escherichia coli (strain K12) (see 5 papers)
28% identity, 99% coverage: 4:794/801 of query aligns to 6:866/1227 of P13009
- C247 (= C205) binding Zn(2+)
- C310 (= C270) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- C311 (= C271) binding Zn(2+); mutation C->A,S: Loss of zinc binding. Loss of catalytic activity.
- E694 (≠ N628) binding methylcob(III)alamin
- GDVHD 756:760 (≠ GDIHD 690:694) binding methylcob(III)alamin
- D757 (= D691) mutation to E: Decreases activity by about 70%.; mutation to N: Decreases activity by about 45%.
- H759 (= H693) binding axial binding residue; mutation to G: Loss of catalytic activity.
- S804 (= S738) binding methylcob(III)alamin
- T808 (= T742) binding methylcob(III)alamin
- S810 (≠ T744) mutation to A: Decreases activity by about 40%.
- A860 (= A788) binding methylcob(III)alamin
Sites not aligning to the query:
- 1 modified: Initiator methionine, Removed
- 946 binding S-adenosyl-L-methionine
- 1134 binding S-adenosyl-L-methionine
- 1189:1190 binding S-adenosyl-L-methionine
Q99707 Methionine synthase; MS; 5-methyltetrahydrofolate--homocysteine methyltransferase; Cobalamin-dependent methionine synthase; Vitamin-B12 dependent methionine synthase; EC 2.1.1.13 from Homo sapiens (Human) (see 6 papers)
28% identity, 98% coverage: 4:785/801 of query aligns to 16:877/1265 of Q99707
- R61 (≠ L35) natural variant: R -> K
- C255 (≠ V200) to Y: in dbSNP:rs1140598
- GSR 382:384 (≠ GRS 325:327) binding (6S)-5,6,7,8-tetrahydrofolate
- D449 (= D389) binding (6S)-5,6,7,8-tetrahydrofolate
- N470 (= N410) binding (6S)-5,6,7,8-tetrahydrofolate
- D537 (= D474) binding (6S)-5,6,7,8-tetrahydrofolate
- N579 (= N511) binding (6S)-5,6,7,8-tetrahydrofolate
- R585 (≠ P517) binding (6S)-5,6,7,8-tetrahydrofolate
- R591 (= R519) binding (6S)-5,6,7,8-tetrahydrofolate
Sites not aligning to the query:
- 919 D → G: in dbSNP:rs1805087
- 963 D→E: Decreases binding to MTRR; when associated with N-1071.
- 1071 K→N: Decreases binding to MTRR; when associated with E-963.
4cczA Crystal structure of human 5-methyltetrahydrofolate-homocysteine methyltransferase, the homocysteine and folate binding domains
27% identity, 70% coverage: 13:571/801 of query aligns to 13:609/611 of 4cczA
- binding (6s)-5,6,7,8-tetrahydrofolate: E336 (= E319), G342 (= G325), R344 (≠ S327), N430 (= N410), M458 (≠ L436), D497 (= D474), G536 (= G508), S538 (= S510), N539 (= N511), F542 (= F514), R545 (≠ P517), R551 (= R519)
2ycjA Methyltransferase bound with methyltetrahydrofolate (see paper)
39% identity, 28% coverage: 316:540/801 of query aligns to 12:237/271 of 2ycjA
- binding 5-methyl-5,6,7,8-tetrahydrofolic acid: M20 (≠ T324), F21 (≠ G325), D84 (= D389), N105 (= N410), D169 (= D474), G205 (= G508), N208 (= N511), Q211 (≠ F514), R216 (= R519), I236 (= I539)
2yciX Methyltransferase native (see paper)
39% identity, 28% coverage: 316:540/801 of query aligns to 12:237/271 of 2yciX