SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012101375.1 NCBI__GCF_000016505.1:WP_012101375.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

7d7oB Crystal structure of cystathionine gamma-lyase from bacillus cereus atcc 14579 (see paper)
60% identity, 99% coverage: 1:378/382 of query aligns to 1:376/377 of 7d7oB

query
sites
7d7oB

M

M

K

R

I

A

E

K

S

T

L

K

L

L

I

I

H

H

G

G

G

I

I

R

D

I

G

G

D

E

K

P

I

S

T

T

G

G

A

S

V

V

S

N

V

V

P

P

I

I

Y

Y

Q

Q

T

T

S

S

T

T

Y

Y

K

K

Q

Q

E

E

A

A

L

V

G

G

K

K

N

H

S

Q

G

G

Y

Y

E

E

Y
|
Y

S

S

R
|
R

T

T

G

G

N

N

P

P

T

T

R

R

E

A

A

A

V

L

E

E

K

E

L

M

I

I

A

A

D

V

L

L

E

E

E

N

G

G

C

H

R

A

G

G

F

F

A

A

F

F

A

G

S

S

G
|
G

L
x
M

A

A

A

A

I

I

S

T

A

A

V

T

L

I

M

M

L

L

F

F

K

S

S

K

G

G

D

D

K

H

V

V

I

I

L

L

S

T

S

D

N

D

V

V

Y
|
Y

G

G

G

G

T

T

F

Y

R

R

V

V

V

I

D

T

K

K

I

V

F

L

K

N

N

R

F

F

N

G

L

I

E

E

Y

H

E

T

L

F

V

V

D

D

T

T

S

T

D

N

L

L

E

E

K

E

V

V

R

E

E

E

S

A

L

I

N

R

R

-

N

-

Q

P

N

N

V

T

K

K

A

A

I

I

F

Y

I

V

E

E

T

T

P

P

T

T

N

N

P

P

L

L

M

L

T

K

I

I

T

T

D

D

I

I

A

K

K

K

I

I

S

S

E

T

I

L

A

A

R

K

E

E

N

K

K

D

V

L

M

L

T

T

I

I

V

I

D
|
D

N

N

T
|
T

F

F

M

M

T

T

P

P

Y

Y

L

W

Q

Q

K

S

P

P

I

I

K

S

L

L

G

G

A

A

D

D

I

I

V

V

L

L

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

V

A

A

G

G

L

L

L

V

V

V

V

V

N

N

N

S

E

P

E

Q

L

L

G

A

E

E

K

D

I

L

H

H

F

F

I

V

Q

Q

N

N

S

S

T

T

G

G

G

G

I

I

L

L

N

G

P

P

F

Q

D

D

S

S

W

F

L

L

L

L

I

L

R

R

G

G

I

L

K

K

T

T

L

L

S

G

V

I

R

R

M

M

D

E

R

E

H

H

D

E

E

T

N

N

A

S

N

R

Y

A

T

I

A

A

E

E

F

F

L

L

K

N

G

N

N

H

N

P

L

K

I

V

D

N

K

K

I

V

Y

Y

Y

Y

P

P

G

G

F

L

L

E

N

S

H

H

P

Q

D

N

H

H

E

E

I

L

Q

A

K

T

S

E

Q

Q

A

A

R

N

G

G

Y

F

G

G

A

A

I

I

I

I

S

S

F

F

V

D

L

V

K

D

D

S

E

E

L

E

D

T

I

L

N

N

K

K

F

V

F

L

K

E

G

K

L

L

K

Q

L

Y

I

F

T

T

L

L

G

A

E

E

S

S

L

L

G

G

G

A

V

V

E

E

S

S

L

L

V

I

C

S

H

I

P

P

A

S

T

Q

M

M

T

T

H

H

A

A

A

S

I

I

P

P

Y

A

E

D

I

R

R

R

Q

K

E

E

I

L

G

G

I

I

E

T

D

D

G

T

L

L

I

I

R

R

L

I

S

S

V

V

G

G

L

I

E

E

N

D

K

G

E

E

D

D

L

L

I

I

E

E

D

D

L

L

K

A

N

Q

A

A

L

L

binding pyridoxal-5'-phosphate: Y44 (= Y44), R46 (= R46), G74 (= G74), M75 (≠ L75), Y98 (= Y98), D170 (= D172), T172 (= T174), S192 (= S194), T194 (= T196), K195 (= K197)

4l0oH Structure determination of cystathionine gamma-synthase from helicobacter pylori
55% identity, 99% coverage: 1:378/382 of query aligns to 1:370/373 of 4l0oH

query
sites
4l0oH

M

M

K

R

I

M

E

Q

S

T

L

K

L

L

I

I

H

H

G

G

G

G

I

I

D

S

G

E

D

D

K

A

I

T

T

T

G

G

A

A

V

V

S

S

V

V

P

P

I

I

Y

Y

Q

Q

T

T

S

S

T

T

Y

Y

K

R

Q

Q

E

D

A

-

L

-

G

-

K

-

N

-

S

-

G

G

Y

Y

E

E

Y
|
Y

S

S

R
|
R

T

S

G

G

N

N

P

P

T

T

R

R

E

F

A

A

V

L

E

E

K

E

L

L

I

I

A

A

D

D

L

L

E

E

E

G

G

G

C

V

R

K

G

G

F

F

A

A

F

F

A

A

S
|
S

G
|
G

L
|
L

A

A

A

G

I

I

S

H

A

A

V

V

L

F

M

S

L

L

F

L

K

Q

S

S

G

G

D

D

K

H

V

V

I

L

L

L

S

G

S

D

N

D

V

V

Y
|
Y

G

G

G

G

T

T

F

F

R

R

V

L

V

F

D

N

K

Q

I

V

F

L

K

V

N

K

F

N

N

G

L

L

E

S

Y

C

E

T

L

I

V

I

D

D

T

T

S

S

D

D

L

I

E

S

K

Q

V

I

R

K

E

K

S

A

L

I

N

K

R

-

N

-

Q

P

N

N

V

T

K

K

A

A

I

L

F

Y

I

L

E

E

T

T

P

P

T

S

N

N

P

P

L

L

M

L

T

K

I

I

T

T

D

D

I

L

A

A

K

Q

I

C

S

A

E

S

I

V

A

A

R

K

E

D

N

H

K

G

V

L

M

L

T

T

I

I

V

V

D
|
D

N

N

T

T

F

F

M

A

T

T

P

P

Y

Y

L

Y

Q

Q

K

N

P

P

I

L

K

L

L

L

G

G

A

A

D

D

I

I

V

V

L

A

H

H

S
|
S

A

G

T
|
T

K
|
K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

V

A

A

G

G

L

L

L

V

V

T

V

T

N

N

N

N

E

E

E

A

L

L

G

A

E

Q

K

E

I

I

H

A

F

F

I

F

Q

Q

N

N

S

A

T

I

G

G

G

G

I

V

L

L

N

G

P

P

F

Q

D

D

S

S

W

W

L

L

L

L

I

Q

R

R

G

G

I

I

K

K

T

T

L

L

S

G

V

L

R

R

M

M

D

E

R

A

H

H

D

Q

E

K

N

N

A

A

N

L

Y

C

T

V

A

A

E

E

F

F

L

L

K

E

G

K

N

H

N

P

L

K

I

V

D

E

K

R

I

V

Y

Y

Y

Y

P

P

G

G

F

L

L

P

N

T

H

H

P

P

D

N

H

Y

E

E

I

L

Q

A

K

K

S

K

Q

Q

A

M

R

R

G

G

Y

F

G

S

A

G

I

M

I

L

S

S

F

F

V

T

L

L

K

K

D

N

E

D

L

S

D

E

I

A

N

V

K

A

F

F

F

V

K

E

G

S

L

L

K

K

L

L

I

F

T

I

L

L

G

G

E

E

S

S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

C

G

H

I

P

P

A

A

T

F

M

M

T

T

H

H

A

A

A

C

I

I

P

P

Y

K

E

T

I

Q

R

R

Q

E

E

A

I

A

G

G

I

I

E

R

D

D

G

G

L

L

I

V

R

R

L

L

S

S

V

V

G

G

L

I

E

E

N

H

K

E

E

Q

D

D

L

L

I

L

E

E

D

D

L

L

K

E

N

Q

A

A

L

F

active site: R40 (= R46), Y92 (= Y98), D164 (= D172), K189 (= K197)
binding pyridoxal-5'-phosphate: Y38 (= Y44), R40 (= R46), S67 (= S73), G68 (= G74), L69 (= L75), Y92 (= Y98), D164 (= D172), S186 (= S194), T188 (= T196), K189 (= K197)

6le4A Crystal structure of cystathionine gamma-lyase from lactobacillus plantarum complexed with cystathionine (see paper)
54% identity, 98% coverage: 1:375/382 of query aligns to 1:372/380 of 6le4A

query
sites
6le4A

M

M

K

K

I

F

E

E

S

T

L

Q

L

L

I

I

H

H

G

G

G

G

I

I

D

S

G

E

D

D

K

A

I

T

T

T

G

G

A

A

V

T

S

S

V

V

P

P

I

I

Y

Y

Q

M

T

A

S

S

T

T

Y

F

K

R

Q

Q

E

T

A

K

L

I

G

G

K

Q

N

N

S

Q

G

-

Y

Y

E
|
E

Y
|
Y

S

S

R
|
R

T
|
T

G

G

N

N

P

P

T

T

R

R

E

A

A

A

V

V

E

E

K

A

L

L

I

I

A

A

D

T

L

L

E

E

E

H

G

G

C

S

R

A

G

G

F

F

A

A

F

F

A

A

S
|
S

G
|
G

L
x
S

A

A

A

A

I

I

S

N

A

T

V

V

L

F

M

S

L

L

F

F

K

S

S

A

G

G

D

D

K

H

V

I

I

I

L

V

S

G

S

N

N

D

V

V

Y
|
Y

G

G

G

G

T

T

F

F

R
|
R

V

L

V

I

D

D

K

A

I

V

F

L

K

K

N

H

F

F

N

G

L

M

E

T

Y

F

E

T

L

A

V

V

D

D

T

T

S

R

D

D

L

L

E

A

K

A

V

V

R

E

E

A

S

A

L

I

N

T

R

-

N

-

Q

P

N

T

V

T

K

K

A

A

I

I

F

Y

I

L

E

E

T

T

P

P

T

T

N
|
N

P

P

L

L

M

L

T

H

I

I

T

T

D

D

I

I

A

A

K

A

I

I

S

A

E

K

I

L

A

A

R

Q

E

A

N

H

K

D

V

L

M

L

T

S

I

I

V

I

D
|
D

N

N

T

T

F

F

M

A

T

S

P

P

Y

Y

L

V

Q

Q

K

K

P

P

I

L

K

D

L

L

G

G

A

V

D

D

I

I

V

V

L

L

H

H

S
|
S

A

A

T

S

K

A

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

I

A

G

G

G

L

L

L

V

V

V

V

T

N

K

N

T

E

P

E

A

L

L

G

G

E

E

K

K

I

I

H

G

F

Y

I

L

Q

Q

N
|
N

S

A

T

I

G

G

G

S

I

I

L

L

N

A

P

P

F

Q

D

E

S

S

W

W

L

L

L

L

I

Q

R

R

G

G

I

M

K

K

T

T

L

L

S

A

V

L

R

R

M

M

D

Q

R

A

H

H

D

L

E

N

N

N

A

A

N

A

Y

K

T

I

A

F

E

T

F

Y

L

L

K

K

G

S

N

H

N

P

L

A

I

V

D

T

K

K

I

I

Y

Y

Y

Y

P

P

G

G

F

D

L

P

N

D

H

N

P

P

D

D

H

F

E

S

I

I

Q

A

K

K

S

Q

Q

Q

A

M

R

N

G

G

Y

F

G

G

A

A

I

M

I

I

S

S

F

F

V

E

L

L

K

Q

D

P

E

G

L

M

D

N

I

P

N

Q

K

T

F

F

F

V

K

E

G

H

L

L

K

Q

L

V

I

I

T

T

L

L

G

A

E
|
E

S
|
S

L
|
L

G

G

G

A

V

L

E

E

S

S

L

L

V

I

C

E

H

I

P

P

A

A

T

L

M

M

T
|
T

H

H

A

G

A

A

I

I

P

P

Y

R

E

T

I

I

R

R

Q

L

E

Q

I

N

G

G

I

I

E

K

D

D

G

E

L

L

I

I

R
|
R

L

L

S

S

V

V

G

G

L

V

E

E

N

A

K

S

E

D

D

D

L

L

I

L

E

A

D

D

L

L

K

E

binding (2~{S})-4-[(2~{R})-2-azanyl-3-oxidanyl-3-oxidanylidene-propyl]sulfanyl-2-[(~{E})-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]butanoic acid: E42 (= E43), Y43 (= Y44), R45 (= R46), T46 (= T47), S72 (= S73), G73 (= G74), S74 (≠ L75), Y97 (= Y98), R102 (= R103), N144 (= N147), D169 (= D172), S191 (= S194), N223 (= N226), E320 (= E323), S321 (= S324), L322 (= L325), T336 (= T339), R356 (= R359)

6ldoA Crystal structure of cystathionine gamma-lyase from lactobacillus plantarum complexed with l-serine (see paper)
54% identity, 98% coverage: 1:375/382 of query aligns to 1:372/381 of 6ldoA

query
sites
6ldoA

M

M

K

K

I

F

E

E

S

T

L

Q

L

L

I

I

H

H

G

G

G

G

I

I

D

S

G

E

D

D

K

A

I

T

T

T

G

G

A

A

V

T

S

S

V

V

P

P

I

I

Y

Y

Q

M

T

A

S

S

T

T

Y

F

K

R

Q

Q

E

T

A

K

L

I

G

G

K

Q

N

N

S

Q

G

-

Y

Y

E

E

Y
|
Y

S

S

R
|
R

T

T

G

G

N

N

P

P

T

T

R

R

E

A

A

A

V

V

E

E

K

A

L

L

I

I

A

A

D

T

L

L

E

E

E

H

G

G

C

S

R

A

G

G

F

F

A

A

F

F

A

A

S

S

G
|
G

L
x
S

A

A

A

A

I

I

S

N

A

T

V

V

L

F

M

S

L

L

F

F

K

S

S

A

G

G

D

D

K

H

V

I

I

I

L

V

S

G

S

N

N

D

V

V

Y
|
Y

G

G

G

G

T

T

F

F

R

R

V

L

V

I

D

D

K

A

I

V

F

L

K

K

N

H

F

F

N

G

L

M

E

T

Y

F

E

T

L

A

V

V

D

D

T

T

S

R

D

D

L

L

E

A

K

A

V

V

R

E

E

A

S

A

L

I

N

T

R

-

N

-

Q

P

N

T

V

T

K

K

A

A

I

I

F

Y

I

L

E

E

T

T

P

P

T

T

N
|
N

P

P

L

L

M

L

T

H

I

I

T

T

D

D

I

I

A

A

K

A

I

I

S

A

E

K

I

L

A

A

R

Q

E

A

N

H

K

D

V

L

M

L

T

S

I

I

V

I

D
|
D

N

N

T

T

F

F

M

A

T

S

P

P

Y

Y

L

V

Q

Q

K

K

P

P

I

L

K

D

L

L

G

G

A

V

D

D

I

I

V

V

L

L

H

H

S
|
S

A

A

T
x
S

K

A

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

I

A

G

G

G

L

L

L

V

V

V

V

T

N

K

N

T

E

P

E

A

L

L

G

G

E

E

K

K

I

I

H

G

F

Y

I

L

Q

Q

N

N

S

A

T

I

G

G

G

S

I

I

L

L

N

A

P

P

F

Q

D

E

S

S

W

W

L

L

L

L

I

Q

R

R

G

G

I

M

K

K

T

T

L

L

S

A

V

L

R

R

M

M

D

Q

R

A

H

H

D

L

E

N

N

N

A

A

N

A

Y

K

T

I

A

F

E

T

F

Y

L

L

K

K

G

S

N

H

N

P

L

A

I

V

D

T

K

K

I

I

Y

Y

Y

Y

P

P

G

G

F

D

L

P

N

D

H

N

P

P

D

D

H

F

E

S

I

I

Q

A

K

K

S

Q

Q

Q

A

M

R

N

G

G

Y

F

G

G

A

A

I

M

I

I

S

S

F

F

V

E

L

L

K

Q

D

P

E

G

L

M

D

N

I

P

N

Q

K

T

F

F

F

V

K

E

G

H

L

L

K

Q

L

V

I

I

T

T

L

L

G

A

E

E

S
|
S

L
|
L

G

G

G

A

V

L

E

E

S

S

L

L

V

I

C

E

H

I

P

P

A

A

T

L

M

M

T
|
T

H

H

A

G

A

A

I

I

P

P

Y

R

E

T

I

I

R

R

Q

L

E

Q

I

N

G

G

I

I

E

K

D

D

G

E

L

L

I

I

R
|
R

L

L

S

S

V

V

G

G

L

V

E

E

N

A

K

S

E

D

D

D

L

L

I

L

E

A

D

D

L

L

K

E

binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: Y43 (= Y44), R45 (= R46), G73 (= G74), S74 (≠ L75), Y97 (= Y98), N144 (= N147), D169 (= D172), S191 (= S194), S193 (≠ T196), S321 (= S324), L322 (= L325), T336 (= T339), R356 (= R359)

7mcyH Crystal structure of staphylococcus aureus cystathionine gamma lyase, holoenzyme with bound nl3 (see paper)
51% identity, 98% coverage: 7:379/382 of query aligns to 7:378/380 of 7mcyH

query
sites
7mcyH

L

L

I

I

H
|
H

G
|
G

G

G

I

H

D

T

G

T

D

D

K

D

I

Y

T

T

G

G

A

A

V

V

S

T

V

T

P

P

I

I

Y

Y

Q

Q

T

T

S

S

T

T

Y

Y

K

L

Q

Q

E

D

A

D

L

I

G

G

K

D

-

L

N

R

S

Q

G

G

Y

Y

E

E

Y

Y

S

S

R

R

T

T

G

A

N

N

P

P

T

T

R

R

E

S

A

S

V

V

E

E

K

S

L

V

I

I

A

A

D

T

L

L

E

E

E

N

G

G

C

K

R

H

G

G

F

F

A

A

F

F

A

S

S

S

G

G

L

V

A

A

A

A

I

I

S

S

A

A

V

V

L

V

M

M

L

L

F

L

K

D

S

K

G

G

D

D

K

H

V

I

I

I

L

L

S

N

S

S

N
x
D

V
|
V

Y
|
Y

G
|
G

G

G

T

T

F
x
Y

R

R

V

A

V

L

D

T

K

K

I

V

F

F

K

T

N

R

F

F

N

G

L

I

E

E

Y

V

E

D

L

F

V

V

D

D

T

T

S

T

D

H

L

T

E

D

K

S

V

I

R

V

E

Q

S

A

L

I

N

R

R

-

N

-

Q

P

N

T

V

T

K

K

A

M

I

L

F

F

I

I

E

E

T

T

P

P

T

S

N
|
N

P
|
P

L

L

M

L

T

R

I

V

T

T

D

D

I

I

A

K

K

K

I

S

S

A

E

E

I

I

A

A

R

K

E

E

N

H

K

G

V

L

M

I

T

S

I

V

V

V

D

D

N

N

T

T

F

F

M

M

T

T

P

P

Y

Y

L

Y

Q

Q

K

N

P

P

I

L

K

D

L

L

G

G

A

I

D

D

I

I

V

V

L

L

H

H

S

S

A

A

T

T

K

K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

V

A

A

G

G

L

L

L

V

V

A

V

T

N

S

N

D

E

D

E

K

L

L

G

A

E

E

K

R

I

L

H

A

F

F

I

I

Q

S

N

N

S

S

T

T

G

G

G

G

I

I

L

L

N

G

P

P

F

Q

D

D

S

S

W

Y

L

L

L

L

I

V

R

R

G

G

I

I

K

K

T

T

L

L

S

G

V

L

R

R

M

M

D

E

R

Q

H

I

D

N

E

R

N

S

A

V

N

I

Y

E

T

I

A

I

E

K

F

M

L

L

K

Q

G

A

N

H

N

P

L

A

I

V

D

Q

K

Q

I

V

Y

F

Y

H

P

P

G

S

F

I

L

E

N

S

H

H

P

L

D

N

H

H

E

D

I

V

Q

H

K

M

S

A

Q

Q

A

A

R

D

G

G

Y

H

G

T

A

G

I

V

I

I

S

A

F

F

V

E

L

V

K

K

D

N

E

T

L

E

D

S

I

A

N

K

K

Q

F

L

F

I

K

K

G

A

L

T

K

S

L

Y

I

Y

T

T

L

L

G

A

E

E

S

S

L

L

G

G

G

A

V

V

E

E

S

S

L

L

V

I

C

S

H

V

P

P

A

A

T

L

M

M

T
|
T

H
|
H

A

A

A

S

I
|
I

P

P

Y

A

E

D

I

I

R

R

Q

A

E

K

I

E

G

G

I

I

E

T

D

D

G

G

L

L

I

V

R

R

L

I

S

S

V

V

G

G

L

I

E

E

N

D

K

T

E

E

D

D

L

L

I

V

E

D

D

D

L

L

K

K

N

Q

A

A

L

L

E

D

binding 3-{[6-(7-chloro-1-benzothiophen-2-yl)-1H-indol-1-yl]methyl}-1H-pyrazole-5-carboxylic acid: D97 (≠ N96), V98 (= V97), Y99 (= Y98), G100 (= G99), Y103 (≠ F102), N146 (= N147), P147 (= P148), T338 (= T339), H339 (= H340), I342 (= I343)
binding pyridoxal-5'-phosphate: H9 (= H9), G10 (= G10)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 1, 6

7mcuH Crystal structure of staphylococcus aureus cystathionine gamma lyase, holoenzyme with bound nl2 (see paper)
51% identity, 98% coverage: 7:379/382 of query aligns to 7:378/380 of 7mcuH

query
sites
7mcuH

L

L

I

I

H
|
H

G
|
G

G

G

I

H

D

T

G

T

D

D

K

D

I

Y

T

T

G

G

A

A

V

V

S

T

V

T

P

P

I

I

Y

Y

Q

Q

T

T

S

S

T

T

Y

Y

K

L

Q

Q

E

D

A

D

L

I

G

G

K

D

-

L

N

R

S

Q

G

G

Y

Y

E

E

Y

Y

S

S

R

R

T

T

G

A

N

N

P

P

T

T

R

R

E

S

A

S

V

V

E

E

K

S

L

V

I

I

A

A

D

T

L

L

E

E

E

N

G

G

C

K

R

H

G

G

F

F

A

A

F

F

A

S

S

S

G

G

L

V

A

A

A

A

I

I

S

S

A

A

V

V

L

V

M

M

L

L

F

L

K

D

S

K

G

G

D

D

K

H

V

I

I

I

L

L

S

N

S

S

N

D

V

V

Y

Y

G
|
G

G

G

T

T

F
x
Y

R

R

V

A

V

L

D

T

K

K

I

V

F

F

K

T

N

R

F

F

N

G

L

I

E

E

Y

V

E

D

L

F

V

V

D

D

T

T

S

T

D

H

L

T

E

D

K

S

V

I

R

V

E

Q

S

A

L

I

N

R

R

-

N

-

Q

P

N

T

V

T

K

K

A

M

I

L

F

F

I

I

E

E

T

T

P

P

T

S

N

N

P

P

L

L

M

L

T

R

I

V

T

T

D

D

I

I

A

K

K

K

I

S

S

A

E

E

I

I

A

A

R

K

E

E

N

H

K

G

V

L

M

I

T

S

I

V

V

V

D

D

N

N

T

T

F

F

M

M

T

T

P

P

Y

Y

L

Y

Q

Q

K

N

P

P

I

L

K

D

L

L

G

G

A

I

D

D

I

I

V

V

L

L

H

H

S

S

A

A

T

T

K

K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

V

A

A

G

G

L

L

L

V

V

A

V

T

N

S

N

D

E

D

E

K

L

L

G

A

E

E

K

R

I

L

H

A

F

F

I

I

Q

S

N

N

S

S

T

T

G

G

G

G

I

I

L

L

N

G

P

P

F

Q

D

D

S

S

W

Y

L

L

L

L

I

V

R

R

G

G

I

I

K

K

T

T

L

L

S

G

V

L

R

R

M

M

D

E

R

Q

H

I

D

N

E

R

N

S

A

V

N

I

Y

E

T

I

A

I

E

K

F

M

L

L

K

Q

G

A

N

H

N

P

L

A

I

V

D

Q

K

Q

I

V

Y

F

Y

H

P

P

G

S

F

I

L

E

N

S

H

H

P

L

D

N

H

H

E

D

I

V

Q

H

K

M

S

A

Q

Q

A

A

R

D

G

G

Y

H

G

T

A

G

I

V

I

I

S

A

F

F

V

E

L

V

K

K

D

N

E

T

L

E

D

S

I

A

N

K

K

Q

F

L

F

I

K

K

G

A

L

T

K

S

L

Y

I

Y

T

T

L

L

G

A

E

E

S

S

L

L

G

G

G

A

V

V

E

E

S

S

L

L

V

I

C

S

H

V

P

P

A

A

T

L

M

M

T
|
T

H
|
H

A

A

A

S

I
|
I

P

P

Y

A

E

D

I

I

R

R

Q

A

E

K

I
x
E

G

G

I

I

E

T

D

D

G

G

L

L

I

V

R

R

L

I

S

S

V

V

G

G

L

I

E

E

N

D

K

T

E

E

D

D

L

L

I

V

E

D

D

D

L

L

K

K

N

Q

A

A

L

L

E

D

binding pyridoxal-5'-phosphate: H9 (= H9), G10 (= G10)
binding 5-[(6-bromo-1H-indol-1-yl)methyl]-2-methylfuran-3-carboxylic acid: G100 (= G99), Y103 (≠ F102), T338 (= T339), H339 (= H340), I342 (= I343), E350 (≠ I351)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 1, 6

7mctH Crystal structure of staphylococcus aureus cystathionine gamma lyase, holoenzyme with bound nl1 (see paper)
51% identity, 98% coverage: 7:379/382 of query aligns to 7:378/380 of 7mctH

query
sites
7mctH

L

L

I

I

H
|
H

G
|
G

G

G

I

H

D

T

G

T

D

D

K

D

I

Y

T

T

G

G

A

A

V

V

S

T

V

T

P

P

I

I

Y

Y

Q

Q

T

T

S

S

T

T

Y

Y

K

L

Q

Q

E

D

A

D

L

I

G

G

K

D

-

L

N

R

S

Q

G

G

Y

Y

E

E

Y

Y

S

S

R

R

T

T

G

A

N

N

P

P

T

T

R

R

E

S

A

S

V

V

E

E

K

S

L

V

I

I

A

A

D

T

L

L

E

E

E

N

G

G

C

K

R

H

G

G

F

F

A

A

F

F

A

S

S

S

G

G

L

V

A

A

A

A

I

I

S

S

A

A

V

V

L

V

M

M

L

L

F

L

K

D

S

K

G

G

D

D

K

H

V

I

I

I

L

L

S

N

S

S

N

D

V

V

Y

Y

G
|
G

G

G

T

T

F
x
Y

R

R

V

A

V

L

D

T

K

K

I

V

F

F

K

T

N

R

F

F

N

G

L

I

E

E

Y

V

E

D

L

F

V

V

D

D

T

T

S

T

D

H

L

T

E

D

K

S

V

I

R

V

E

Q

S

A

L

I

N

R

R

-

N

-

Q

P

N

T

V

T

K

K

A

M

I

L

F

F

I

I

E

E

T

T

P

P

T

S

N

N

P

P

L

L

M

L

T

R

I

V

T

T

D

D

I

I

A

K

K

K

I

S

S

A

E

E

I

I

A

A

R

K

E

E

N

H

K

G

V

L

M

I

T

S

I

V

V

V

D

D

N

N

T

T

F

F

M

M

T

T

P
|
P

Y
|
Y

L

Y

Q

Q

K

N

P

P

I

L

K

D

L

L

G

G

A

I

D

D

I

I

V

V

L

L

H

H

S

S

A

A

T

T

K

K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

V

A

A

G

G

L

L

L

V

V

A

V

T

N

S

N

D

E

D

E

K

L

L

G

A

E

E

K

R

I

L

H

A

F

F

I

I

Q

S

N

N

S

S

T

T

G

G

G

G

I

I

L

L

N

G

P

P

F

Q

D

D

S

S

W

Y

L

L

L

L

I

V

R

R

G

G

I

I

K

K

T

T

L

L

S

G

V

L

R

R

M

M

D

E

R

Q

H

I

D

N

E

R

N

S

A

V

N

I

Y

E

T

I

A

I

E

K

F

M

L

L

K

Q

G

A

N

H

N

P

L

A

I

V

D

Q

K

Q

I

V

Y

F

Y

H

P

P

G

S

F

I

L

E

N

S

H

H

P

L

D

N

H

H

E

D

I

V

Q

H

K

M

S

A

Q

Q

A

A

R

D

G

G

Y

H

G

T

A

G

I

V

I

I

S

A

F

F

V

E

L

V

K

K

D

N

E

T

L

E

D

S

I

A

N

K

K

Q

F

L

F

I

K

K

G

A

L

T

K

S

L

Y

I

Y

T

T

L

L

G

A

E

E

S

S

L

L

G

G

G

A

V

V

E

E

S

S

L

L

V

I

C

S

H

V

P

P

A

A

T

L

M

M

T
|
T

H
|
H

A

A

A

S

I
|
I

P

P

Y

A

E

D

I

I

R

R

Q

A

E

K

I
x
E

G

G

I

I

E

T

D

D

G

G

L

L

I

V

R

R

L

I

S

S

V

V

G

G

L

I

E

E

N

D

K

T

E

E

D

D

L

L

I

V

E

D

D

D

L

L

K

K

N

Q

A

A

L

L

E

D

binding N-[(6-bromo-1H-indol-1-yl)acetyl]glycine: G100 (= G99), Y103 (≠ F102), P177 (= P178), Y178 (= Y179), T338 (= T339), H339 (= H340), I342 (= I343), E350 (≠ I351)
binding pyridoxal-5'-phosphate: H9 (= H9), G10 (= G10)

Sites not aligning to the query:

7mcqA Crystal structure of staphylococcus aureus cystathionine gamma lyase, aoaa-bound enzyme in dimeric form (see paper)
51% identity, 98% coverage: 7:379/382 of query aligns to 7:378/380 of 7mcqA

query
sites
7mcqA

L

L

I

I

H

H

G

G

G

G

I

H

D

T

G

T

D

D

K

D

I

Y

T

T

G

G

A

A

V

V

S

T

V

T

P

P

I

I

Y

Y

Q

Q

T

T

S

S

T

T

Y

Y

K

L

Q

Q

E

D

A

D

L

I

G

G

K

D

-

L

N

R

S

Q

G

G

Y

Y

E

E

Y
|
Y

S

S

R
|
R

T

T

G

A

N

N

P

P

T

T

R

R

E

S

A

S

V

V

E

E

K

S

L

V

I

I

A

A

D

T

L

L

E

E

E

N

G

G

C

K

R

H

G

G

F

F

A

A

F

F

A

S

S
|
S

G
|
G

L
x
V

A

A

A

A

I

I

S

S

A

A

V

V

L

V

M

M

L

L

F

L

K

D

S

K

G

G

D

D

K

H

V

I

I

I

L

L

S

N

S

S

N

D

V

V

Y
|
Y

G

G

G

G

T

T

F

Y

R

R

V

A

V

L

D

T

K

K

I

V

F

F

K

T

N

R

F

F

N

G

L

I

E

E

Y

V

E

D

L

F

V

V

D

D

T

T

S

T

D

H

L

T

E

D

K

S

V

I

R

V

E

Q

S

A

L

I

N

R

R

-

N

-

Q

P

N

T

V

T

K

K

A

M

I

L

F

F

I

I

E

E

T

T

P

P

T

S

N

N

P

P

L

L

M

L

T

R

I

V

T

T

D

D

I

I

A

K

K

K

I

S

S

A

E

E

I

I

A

A

R

K

E

E

N

H

K

G

V

L

M

I

T

S

I

V

V

V

D
|
D

N

N

T

T

F

F

M

M

T

T

P

P

Y

Y

L

Y

Q

Q

K

N

P

P

I

L

K

D

L

L

G

G

A

I

D

D

I

I

V

V

L

L

H

H

S
|
S

A

A

T
|
T

K
|
K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

V

A

A

G

G

L

L

L

V

V

A

V

T

N

S

N

D

E

D

E

K

L

L

G

A

E

E

K

R

I

L

H

A

F

F

I

I

Q

S

N

N

S

S

T

T

G

G

G

G

I

I

L

L

N

G

P

P

F

Q

D

D

S

S

W

Y

L

L

L

L

I

V

R

R

G

G

I

I

K

K

T

T

L

L

S

G

V

L

R

R

M

M

D

E

R

Q

H

I

D

N

E

R

N

S

A

V

N

I

Y

E

T

I

A

I

E

K

F

M

L

L

K

Q

G

A

N

H

N

P

L

A

I

V

D

Q

K

Q

I

V

Y

F

Y

H

P

P

G

S

F

I

L

E

N

S

H

H

P

L

D

N

H

H

E

D

I

V

Q

H

K

M

S

A

Q

Q

A

A

R

D

G

G

Y

H

G

T

A

G

I

V

I

I

S

A

F

F

V

E

L

V

K

K

D

N

E

T

L

E

D

S

I

A

N

K

K

Q

F

L

F

I

K

K

G

A

L

T

K

S

L

Y

I

Y

T

T

L

L

G

A

E
|
E

S
|
S

L

L

G

G

G

A

V

V

E

E

S

S

L

L

V

I

C

S

H

V

P

P

A

A

T

L

M

M

T

T

H

H

A

A

A

S

I

I

P

P

Y

A

E

D

I

I

R

R

Q

A

E

K

I

E

G

G

I

I

E

T

D

D

G

G

L

L

I

V

R

R

L

I

S

S

V

V

G

G

L

I

E

E

N

D

K

T

E

E

D

D

L

L

I

V

E

D

D

D

L

L

K

K

N

Q

A

A

L

L

E

D

binding 4'-deoxy-4'-acetylyamino-pyridoxal-5'-phosphate: Y45 (= Y44), R47 (= R46), S74 (= S73), G75 (= G74), V76 (≠ L75), Y99 (= Y98), D171 (= D172), S193 (= S194), T195 (= T196), K196 (= K197), E322 (= E323), S323 (= S324)

7mcbH Crystal structure of staphylococcus aureus cystathionine gamma lyase holoenzyme (see paper)
51% identity, 98% coverage: 7:379/382 of query aligns to 7:378/380 of 7mcbH

query
sites
7mcbH

L

L

I

I

H
|
H

G
|
G

G

G

I

H

D

T

G

T

D

D

K

D

I

Y

T

T

G

G

A

A

V

V

S

T

V

T

P

P

I

I

Y

Y

Q

Q

T

T

S

S

T

T

Y

Y

K

L

Q

Q

E

D

A

D

L

I

G

G

K

D

-

L

N

R

S

Q

G

G

Y

Y

E

E

Y

Y

S

S

R

R

T

T

G

A

N

N

P

P

T

T

R

R

E

S

A

S

V

V

E

E

K

S

L

V

I

I

A

A

D

T

L

L

E

E

E

N

G

G

C

K

R

H

G

G

F

F

A

A

F

F

A

S

S

S

G

G

L

V

A

A

A

A

I

I

S

S

A

A

V

V

L

V

M

M

L

L

F

L

K

D

S

K

G

G

D

D

K

H

V

I

I

I

L

L

S

N

S

S

N

D

V

V

Y

Y

G

G

G

G

T

T

F

Y

R

R

V

A

V

L

D

T

K

K

I

V

F

F

K

T

N

R

F

F

N

G

L

I

E

E

Y

V

E

D

L

F

V

V

D

D

T

T

S

T

D

H

L

T

E

D

K

S

V

I

R

V

E

Q

S

A

L

I

N

R

R

-

N

-

Q

P

N

T

V

T

K

K

A

M

I

L

F

F

I

I

E

E

T

T

P

P

T

S

N

N

P

P

L

L

M

L

T

R

I

V

T

T

D

D

I

I

A

K

K

K

I

S

S

A

E

E

I

I

A

A

R

K

E

E

N

H

K

G

V

L

M

I

T

S

I

V

V

V

D

D

N

N

T

T

F

F

M

M

T

T

P

P

Y

Y

L

Y

Q

Q

K

N

P

P

I

L

K

D

L

L

G

G

A

I

D

D

I

I

V

V

L

L

H

H

S

S

A

A

T

T

K

K

Y

Y

L

L

G

G

G

G

H

H

S

S

D

D

L

V

I

V

A

A

G

G

L

L

L

V

V

A

V

T

N

S

N

D

E

D

E

K

L

L

G

A

E

E

K

R

I

L

H

A

F

F

I

I

Q

S

N

N

S

S

T

T

G

G

G

G

I

I

L

L

N

G

P

P

F

Q

D

D

S

S

W

Y

L

L

L

L

I

V

R

R

G

G

I

I

K

K

T

T

L

L

S

G

V

L

R

R

M

M

D

E

R

Q

H

I

D

N

E

R

N

S

A

V

N

I

Y

E

T

I

A

I

E

K

F

M

L

L

K

Q

G

A

N

H

N

P

L

A

I

V

D

Q

K

Q

I

V

Y

F

Y

H

P

P

G

S

F

I

L

E

N

S

H

H

P

L

D

N

H

H

E

D

I

V

Q

H

K

M

S

A

Q

Q

A

A

R

D

G

G

Y

H

G

T

A

G

I

V

I

I

S

A

F

F

V

E

L

V

K

K

D

N

E

T

L

E

D

S

I

A

N

K

K

Q

F

L

F

I

K

K

G

A

L

T

K

S

L

Y

I

Y

T

T

L

L

G

A

E

E

S

S

L

L

G

G

G

A

V

V

E

E

S

S

L

L

V

I

C

S

H

V

P

P

A

A

T

L

M

M

T

T

H

H

A

A

A

S

I

I

P

P

Y

A

E

D

I

I

R

R

Q

A

E

K

I

E

G

G

I

I

E

T

D

D

G

G

L

L

I

V

R

R

L

I

S

S

V

V

G

G

L

I

E

E

N

D

K

T

E

E

D

D

L

L

I

V

E

D

D

D

L

L

K

K

N

Q

A

A

L

L

E

D

binding pyridoxal-5'-phosphate: H9 (= H9), G10 (= G10)

Sites not aligning to the query:

binding pyridoxal-5'-phosphate: 1, 6

4iyoB Crystal structure of cystathionine gamma lyase from xanthomonas oryzae pv. Oryzae (xometc) in complex with e-site serine, a-site serine, a- site external aldimine structure with aminoacrylate and a-site iminopropionate intermediates (see paper)
50% identity, 99% coverage: 1:378/382 of query aligns to 2:380/381 of 4iyoB

query
sites
4iyoB

M

L

K

S

I

L

E

A

S

T

L

L

L

A

I

I

H

H

G

G

G

G

I

Q

D

S

G

P

D

D

K

P

I

S

T

T

G

G

A

A

V

V

S

M

V

P

P

P

I

I

Y

Y

Q

A

T

T

S

S

T

T

Y

Y

K

A

Q

Q

E

S

A

S

L

P

G

G

K

E

N

H

S

Q

G

G

Y

F

E

E

Y
|
Y

S

S

R
|
R

T
|
T

G

H

N

N

P

P

T

T

R

R

E

F

A

A

V

Y

E

E

K

R

L

C

I

V

A

A

D

A

L

L

E

E

E

G

G

G

C

T

R

R

G

A

F

F

A

A

F

F

A

A

S

S

G

G

L

M

A

A

A

A

I

T

S

S

A

T

V

V

L

M

M

E

L

L

F

L

K

D

S

A

G

G

D

S

K

H

V

V

I

V

L

A

S

M

S

D

N

D

V

L

Y
|
Y

G

G

G

G

T

T

F

F

R
|
R

V

L

V

F

D

E

K

R

I

V

-

R

F

R

K

R

N

T

F

A

N

G

L

L

E

D

Y

F

E

S

L

F

V

V

D

D

T

L

S

T

D

D

L

P

E

A

K

A

V

F

R

K

E

A

S

A

L

I

N

-

R

-

N

R

Q

A

N

D

V

T

K

K

A

M

I

V

F

W

I

I

E

E

T

T

P

P

T

T

N

N

P

P

L

M

M

L

T

K

I

L

T

V

D

D

I

I

A

A

K

A

I

I

S

A

E

V

I

I

A

A

R

R

E

K

N

H

K

G

V

L

M

L

T

T

I

V

V

V

D
|
D

N

N

T

T

F

F

M

A

T

S

P

P

Y

M

L

L

Q

Q

K

R

P

P

I

L

K

S

L

L

G

G

A

A

D

D

I

L

V

V

L

V

H

H

S

S

A

A

T

T

K
|
K

Y

Y

L

L

G

N

G

G

H

H

S

S

D

D

L

M

I

V

A

G

G

G

L

I

L

A

V

V

V

V

-

G

N

D

N

N

E

A

E

E

L

L

G

A

E

E

K
x
Q

I

M

H

A

F
|
F

I

L

Q

Q

N
|
N

S

S

T

I

G

G

G

G

I

V

L

Q

N

G

P

P

F

F

D

D

S

S

W

F

L

L

L

A

I

L

R

R

G

G

I

L

K

K

T

T

L

L

S

P

V

L

R

R

M

M

D

R

R

A

H

H

D

C

E

E

N

N

A

A

N

L

Y

A

T

L

A

A

E

Q

F

W

L

L

K

E

G

T

N

H

N

P

L

A

I

I

D

E

K

K

I

V

Y

I

Y

Y

P

P

G

G

F

L

L

A

N

S

H

H

P

P

D

Q

H

H

E

V

I

L

Q

A

K

K

S

R

Q

Q

A

M

R

S

G

G

Y

F

G

G

A

G

I

I

I

V

S

S

F

I

V

V

L

L

K

K

D

G

E

G

L

F

D

D

I

A

N

A

K

K

-

R

F

F

F

C

K

E

G

K

L

T

K

E

L

L

I

F

T

T

L

L

G

A

E
|
E

S
|
S

L

L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

C

N

H

H

P

P

A

A

T

V

M

M

T
|
T

H

H

A

A

A

S

I

I

P

P

Y

V

E

A

I

R

R

R

Q

E

E

Q

I

L

G

G

I

I

E

S

D

D

G

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

N

D

K

L

E

G

D

D

L

L

I

R

E

G

D

D

L

L

K

E

N

R

A

A

L

L

active site: R47 (= R46), Y99 (= Y98), D172 (= D172), K197 (= K197)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y44), R47 (= R46)
binding amino-acrylate: Y99 (= Y98), K197 (= K197), S326 (= S324), T341 (= T339), R361 (= R359)
binding pyruvic acid: Q221 (≠ K220), F224 (= F223)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y98), R104 (= R103), N227 (= N226), E325 (= E323)

4iy7B Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
50% identity, 99% coverage: 1:378/382 of query aligns to 2:380/381 of 4iy7B

query
sites
4iy7B

M

L

K

S

I

L

E

A

S

T

L

L

L

A

I

I

H

H

G

G

G

G

I

Q

D

S

G

P

D

D

K

P

I

S

T

T

G

G

A

A

V

V

S

M

V

P

P

P

I

I

Y

Y

Q

A

T

T

S

S

T

T

Y

Y

K

A

Q

Q

E

S

A

S

L

P

G

G

K

E

N

H

S

Q

G

G

Y

F

E

E

Y
|
Y

S

S

R
|
R

T
|
T

G

H

N

N

P

P

T

T

R

R

E

F

A

A

V

Y

E

E

K

R

L

C

I

V

A

A

D

A

L

L

E

E

E

G

G

G

C

T

R

R

G

A

F

F

A

A

F

F

A

A

S

S

G
|
G

L
x
M

A

A

A

A

I

T

S

S

A

T

V

V

L

M

M

E

L

L

F

L

K

D

S

A

G

G

D

S

K

H

V

V

I

V

L

A

S

M

S

D

N

D

V

L

Y
|
Y

G

G

G

G

T

T

F

F

R
|
R

V

L

V

F

D

E

K

R

I

V

-

R

F

R

K

R

N

T

F

A

N

G

L

L

E

D

Y

F

E

S

L

F

V

V

D

D

T

L

S

T

D

D

L

P

E

A

K

A

V

F

R

K

E

A

S

A

L

I

N

-

R

-

N

R

Q

A

N

D

V

T

K

K

A

M

I

V

F

W

I

I

E
|
E

T

T

P

P

T

T

N
|
N

P

P

L

M

M

L

T

K

I

L

T

V

D

D

I

I

A

A

K

A

I

I

S

A

E

V

I

I

A

A

R

R

E

K

N

H

K

G

V

L

M

L

T

T

I

V

V

V

D
|
D

N

N

T

T

F

F

M

A

T

S

P

P

Y

M

L

L

Q

Q

K

R

P

P

I

L

K

S

L

L

G

G

A

A

D

D

I

L

V

V

L

V

H

H

S
|
S

A

A

T

T

K
|
K

Y

Y

L

L

G

N

G

G

H

H

S

S

D

D

L

M

I

V

A

G

G

G

L

I

L

A

V

V

V

V

-

G

N

D

N

N

E

A

E

E

L

L

G

A

E

E

K
x
Q

I

M

H

A

F
|
F

I

L

Q

Q

N
|
N

S

S

T

I

G

G

G

G

I

V

L

Q

N

G

P

P

F

F

D

D

S

S

W

F

L

L

L

A

I

L

R

R

G

G

I

L

K

K

T

T

L

L

S

P

V

L

R

R

M

M

D

R

R

A

H

H

D

C

E

E

N

N

A

A

N

L

Y

A

T

L

A

A

E

Q

F

W

L

L

K

E

G

T

N

H

N

P

L

A

I

I

D

E

K

K

I

V

Y

I

Y

Y

P

P

G

G

F

L

L

A

N

S

H

H

P

P

D

Q

H

H

E

V

I

L

Q

A

K

K

S

R

Q

Q

A

M

R

S

G

G

Y

F

G

G

A

G

I

I

I

V

S

S

F

I

V

V

L

L

K

K

D

G

E

G

L

F

D

D

I

A

N

A

K

K

-

R

F

F

F

C

K

E

G

K

L

T

K

E

L

L

I

F

T

T

L

L

G

A

E
|
E

S
|
S

L
|
L

G

G

G

G

V

V

E

E

S

S

L

L

V

V

C

N

H

H

P

P

A

A

T

V

M

M

T
|
T

H

H

A

A

A

S

I

I

P

P

Y

V

E

A

I

R

R

R

Q

E

E

Q

I

L

G

G

I

I

E

S

D

D

G

A

L

L

I

V

R
|
R

L

L

S

S

V

V

G

G

L

I

E

E

N

D

K

L

E

G

D

D

L

L

I

R

E

G

D

D

L

L

K

E

N

R

A

A

L

L

active site: R47 (= R46), Y99 (= Y98), D172 (= D172), K197 (= K197)
binding 2-{[(E)-{3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene]amino}prop-2-enoic acid: Y45 (= Y44), R47 (= R46)
binding (E)-N-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methylidene)-L-serine: G75 (= G74), M76 (≠ L75), Y99 (= Y98), E143 (= E143), N147 (= N147), D172 (= D172), S194 (= S194), K197 (= K197), S326 (= S324), L327 (= L325), T341 (= T339), R361 (= R359)
binding pyruvic acid: Q221 (≠ K220), F224 (= F223)
binding serine: Y45 (= Y44), T48 (= T47), Y99 (= Y98), R104 (= R103), N227 (= N226), E325 (= E323)

4iy7A Crystal structure of cystathionine gamma lyase (xometc) from xanthomonas oryzae pv. Oryzae in complex with e-site serine, a-site external aldimine structure with serine and a-site external aldimine structure with aminoacrylate intermediates (see paper)
50% identity, 99% coverage: 1:378/382 of query aligns to 2:380/381 of 4iy7A

query
sites
4iy7A

M

L

K

S

I

L

E

A

S

T

L

L

L

A

I

I

H

H

G

G

G

G

I

Q

D

S

G

P

D

D

K

P

I

S

T

T

G

G

A

A

V

V

S

M

V

P

P

P

I

I

Y

Y

Q

A

T

T

S

S

T

T

Y

Y

K

A

Q

Q

E

S

A

S

L

P

G

G

K

E

N

H

S

Q

G

G

Y

F

E

E

Y
|
Y

S

S

R
|
R

T
|
T

G

H

N

N

P

P

T

T

R

R

E

F

A

A

V

Y

E

E

K

R

L

C

I

V

A

A

D

A

L

L

E

E

E

G

G

G

C

T

R

R

G

A

F

F

A

A

F

F

A

A

S

S

G
|
G

L
x
M

A

A

A

A

I

T

S

S

A

T

V

V

L

M

M

E

L

L

F

L

K

D

S

A

G

G

D

S

K

H

V

V

I

V

L

A

S

M

S

D

N

D

V

L

Y
|
Y

G

G

G

G

T

T

F

F

R
|
R

V

L

V

F

D

E

K

R

I

V

-

R

F

R

K

R

N

T

F

A

N

G

L

L

E

D

Y

F

E

S

L

F

V

V

D

D

T

L

S

T

D

D

L

P

E

A

K

A

V

F

R

K

E

A

S

A

L

I

N

-

R

-

N

R

Q

A

N

D

V

T

K

K

A

M

I

V

F

W

I

I

E
|
E

T

T

P

P

T

T

N
|
N

P

P

L

M

M

L

T

K

I

L

T

V

D

D

I

I

A

A

K

A

I

I

S

A

E

V

I

I

A

A

R

R

E

K

N

H

K

G

V

L

M

L

T

T

I

V

V

V

D
|
D

N

N

T

T

F

F

M

A

T

S

P

P

Y

M

L

L

Q

Q

K

R

P

P

I

L

K

S

L

L

G

G

A

A

D

D

I

L

V

V

L

V

H

H

S
|
S

A

A

T

T

K
|
K

Y

Y

L

L

G

N

G

G

H

H

S

S

D

D

L

M

I

V

A

G

G

G

L

I

L

A

V

V

V

V

-

G

N

D

N

N

E

A

E

E

L

L

G

A

E

E

K

Q

I

M

H

A

F

F

I

L

Q

Q

N
|
N

S

S

T

I

G

G

G

G

I

V

L

Q

N

G

P

P

F

F

D

D

S

S

W

F

L

L

L

A

I

L

R

R

G

G

I

L

K

K

T

T

L

L

S

P

V

L

R

R

M

M

D

R

R

A

H

H

D

C

E

E

N

N

A

A

N

L

Y

A

T

L

A

A

E

Q

F

W

L

L

K

E

G

T

N

H

N

P

L

A

I

I

D

E

K

K

I

V

Y

I

Y

Y

P

P

G

G

F

L

L

A

N

S

H

H

P

P

D

Q

H

H

E

V

I

L

Q

A

K

K

S

R

Q

Q

A

M

R

S

G

G

Y

F

G

G

A

G

I

I

I

V

S

S

F

I

V

V

L

L

K