SitesBLAST
Comparing WP_012101484.1 NCBI__GCF_000016505.1:WP_012101484.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
41% identity, 100% coverage: 1:433/433 of query aligns to 1:435/435 of Q5F8J4
- L45 (≠ R45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
- LS 45:46 (≠ RN 45:46) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).
6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
39% identity, 99% coverage: 2:429/433 of query aligns to 3:429/431 of 6dzsA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), L12 (= L11), G13 (= G12), N14 (= N13), V15 (= V14), V45 (= V44), R46 (= R45), R47 (≠ N46), R52 (= R51), I63 (≠ F64), L78 (≠ V79), M79 (= M80), P84 (= P85), A102 (= A103), K104 (= K105), G306 (= G302), T310 (= T306)
4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
45% identity, 73% coverage: 1:317/433 of query aligns to 2:306/402 of 4pg7A
Sites not aligning to the query:
6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
33% identity, 68% coverage: 1:293/433 of query aligns to 1:281/331 of 6a0sA
- active site: D191 (= D201), K195 (= K205)
- binding l-homoserine: K99 (= K105), N150 (= N159), G151 (= G160), T152 (= T161), Y178 (= Y188), E180 (= E190), D186 (= D196), K195 (= K205)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L11), G12 (= G12), T13 (≠ N13), V14 (= V14), L42 (= L43), V43 (= V44), R44 (= R45), D45 (≠ N46), K48 (= K49), R50 (= R51), A73 (≠ V79), M74 (= M80), A97 (= A103), K99 (= K105), G177 (= G187), E180 (= E190)
Sites not aligning to the query:
2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
33% identity, 68% coverage: 1:293/433 of query aligns to 1:281/331 of 2ejwA
6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
33% identity, 68% coverage: 1:293/433 of query aligns to 1:281/332 of 6a0tB
- active site: D191 (= D201), K195 (= K205)
- binding l-homoserine: N150 (= N159), G151 (= G160), T152 (= T161), Y178 (= Y188), E180 (= E190), D186 (= D196), K195 (= K205)
- binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), G11 (≠ L11), G12 (= G12), T13 (≠ N13), V14 (= V14), L42 (= L43), V43 (= V44), R44 (= R45), D45 (≠ N46), K48 (= K49), R50 (= R51), A73 (≠ V79), M74 (= M80), G75 (= G81), A97 (= A103), N98 (= N104), G177 (= G187), E180 (= E190)
Sites not aligning to the query:
7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
31% identity, 73% coverage: 4:319/433 of query aligns to 1:297/300 of 7f4cA
- binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ M9), G7 (= G10), Y8 (≠ L11), G9 (= G12), N10 (= N13), V11 (= V14), T37 (≠ V44), R38 (= R45), R39 (≠ N46), V72 (= V79), S73 (≠ M80), S74 (≠ G81), T100 (≠ A103), K102 (= K105), G127 (≠ A130), S131 (≠ G134), E185 (= E190), G280 (= G302), A284 (≠ T306)
F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
31% identity, 73% coverage: 4:319/433 of query aligns to 1:301/304 of F9VNG5
- Y8 (≠ L11) binding NADP(+)
- N10 (= N13) binding NADP(+)
- V11 (= V14) binding NADP(+)
- R38 (= R45) binding NADP(+)
- R39 (≠ N46) binding NADP(+)
- S73 (≠ M80) binding NADP(+)
- T100 (≠ A103) binding NADP(+)
- K102 (= K105) binding NADP(+)
- G182 (= G187) binding NADP(+)
- E185 (= E190) binding NADP(+)
- G284 (= G302) binding NADP(+)
Sites not aligning to the query:
5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
32% identity, 73% coverage: 4:319/433 of query aligns to 1:299/302 of 5x9dA
- active site: D196 (= D201), K200 (= K205)
- binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ M9), G7 (= G10), Y8 (≠ L11), G9 (= G12), N10 (= N13), V11 (= V14), T37 (≠ V44), R38 (= R45), V72 (= V79), S73 (≠ M80), S74 (≠ G81), P82 (= P85), T100 (≠ A103), N101 (= N104), K102 (= K105), G127 (≠ A130), S131 (≠ G134), N155 (= N159), G156 (= G160), T157 (= T161), Y183 (= Y188), A184 (= A189), E185 (= E190), D191 (= D196), D196 (= D201), K200 (= K205), A281 (= A301), G282 (= G302), A286 (≠ T306)
4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
28% identity, 72% coverage: 3:315/433 of query aligns to 2:309/319 of 4xb2A
- active site: D211 (= D201), K215 (= K205)
- binding l-homoserine: A171 (≠ G160), S172 (≠ T161), D206 (= D196), K215 (= K205)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ M9), F10 (≠ L11), G11 (= G12), T12 (≠ N13), V13 (= V14), R40 (= R32), V91 (= V79), S92 (≠ M80), S93 (≠ G81), S114 (≠ A103), N115 (= N104), K116 (= K105), S141 (≠ A130), G295 (≠ A301), T300 (= T306)
4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
28% identity, 72% coverage: 3:315/433 of query aligns to 2:309/319 of 4xb1A
- active site: D211 (= D201), K215 (= K205)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ M9), F10 (≠ L11), G11 (= G12), T12 (≠ N13), V13 (= V14), D39 (≠ K31), R40 (= R32), K57 (≠ V44), V91 (= V79), S92 (≠ M80), S93 (≠ G81), S114 (≠ A103), K116 (= K105), S141 (≠ A130), G295 (≠ A301), T300 (= T306)
O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
28% identity, 72% coverage: 3:315/433 of query aligns to 2:309/319 of O58802
- F10 (≠ L11) binding NADPH
- T12 (≠ N13) binding NADPH
- V13 (= V14) binding NADPH
- R40 (= R32) binding NADPH; mutation to A: Increases activity with NADP.
- K57 (≠ V44) binding NADPH; mutation to A: Increases activity with NADP.
- S92 (≠ M80) binding NADPH
- S93 (≠ G81) binding NADPH
- S114 (≠ A103) binding NADPH
- K116 (= K105) binding NADPH
- E140 (= E129) binding Na(+)
- V143 (= V132) binding Na(+)
- A145 (≠ G134) binding Na(+)
- T147 (≠ I136) binding Na(+)
- G296 (= G302) binding NADPH
3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
31% identity, 52% coverage: 2:228/433 of query aligns to 1:236/319 of 3ingA
- active site: D209 (= D201), K213 (= K205)
- binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), T10 (≠ L11), G11 (= G12), N12 (= N13), V13 (= V14), D38 (vs. gap), S39 (vs. gap), K57 (= K47), C85 (≠ D71), T86 (≠ S72), P87 (≠ M80), A112 (= A103), N113 (= N104), K114 (= K105), A139 (= A130), E198 (= E190), S199 (≠ A191)
Sites not aligning to the query:
3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
30% identity, 53% coverage: 1:228/433 of query aligns to 1:239/321 of 3jsaA