SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012101948.1 NCBI__GCF_000016505.1:WP_012101948.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

2ordA Crystal structure of acetylornithine aminotransferase (ec 2.6.1.11) (acoat) (tm1785) from thermotoga maritima at 1.40 a resolution
45% identity, 97% coverage: 10:388/390 of query aligns to 10:391/393 of 2ordA

query
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2ordA

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active site: F134 (= F135), E186 (= E186), D219 (= D219), Q222 (= Q222), K248 (= K248), T276 (= T277), R367 (= R364)
binding pyridoxal-5'-phosphate: G102 (= G102), T103 (≠ A103), F134 (= F135), H135 (= H136), E186 (= E186), D219 (= D219), V221 (≠ I221), Q222 (= Q222), K248 (= K248)

Q9X2A5 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8)
45% identity, 97% coverage: 10:388/390 of query aligns to 2:383/385 of Q9X2A5

query
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Q9X2A5

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GT 94:95 (≠ GA 102:103) binding pyridoxal 5'-phosphate
T268 (= T277) binding pyridoxal 5'-phosphate

Q9M8M7 Acetylornithine aminotransferase, chloroplastic/mitochondrial; ACOAT; Acetylornithine transaminase; AOTA; Protein HOPW1-1-INTERACTING 1; EC 2.6.1.11 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
43% identity, 96% coverage: 11:385/390 of query aligns to 69:451/457 of Q9M8M7

query
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Sites not aligning to the query:

1:41 modified: transit peptide, Chloroplast and mitochondrion
42 modified: N-acetylvaline; in Acetylornithine aminotransferase, chloroplastic

O66442 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Aquifex aeolicus (strain VF5)
47% identity, 97% coverage: 10:387/390 of query aligns to 3:376/376 of O66442

query
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O66442

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-

G

G

K

K

A

N

R

K

N

W

T

K

I

F

L

I

T

S

L

F

K

E

K

N

S

S

F

F

H

H

G

G

R

R

T

T

I

Y

T

G

T

S

L

L

K

S

A

A

G

T

G

G

Q

Q

E

P

K

K

L

F

H

H

K

K

Y

G

F

F

Y

E

P

P

F

L

T

V

E

P

G

G

F

F

K

S

Y

Y

A

A

E

K

A

L

N

N

-

D

V

I

E

D

E

S

L

V

E

Y

K

K

S

L

I

L

D

D

S

E

I

T

C

A

A

G

I

I

M

I

I

I

E

E

P

V

I

I

Q

Q

G

G

E

E

G

G

I

V

N

N

P

E

L

A

S

S

E

E

E

D

F

F

V

L

H

S

K

K

V

L

F

Q

G

E

I

I

A

C

E

K

K

E

E

K

D

D

I

V

L

L

V

L

I

I

C

I

D

D

E

E

I

V

Q

Q

C

T

G

G

I

I

G

G

R

R

T

T

G

G

K

E

I

F

Y

Y

G

A

F

Y

N

Q

N

H

Y

F

G

N

V

L

C

K

P

P

D

D

I

V

I

I

S

A

T

L

A

A

K
|
K

G

G

L

L

G

G

L

V

P

P

I

I

G

G

A

A

V

I

L

L

C

A

N

R

E

E

K

E

L

V

N

A

N

Q

T

S

F

F

E

T

Y

P

G

G

D

S

H

H

G

G

S

S

T
|
T

F

F

G

G

N

N

P

P

V

L

-

A

C

C

A

R

A

A

G

G

A

T

L

V

E

V

V

V

L

D

N

E

I

V

I

-

S

-

E

-

N

E

S

K

F

L

L

L

E

P

E

H

V

V

S

R

E

E

K

V

G

G

K

N

F

Y

V

F

K

K

E

E

Y

K

F

L

K

K

S

E

K

L

N

G

K

K

K

G

N

-

I

-

L

-

E

K

V

V

R

K

G

G

M

R

G

G

L

L

M

M

I

L

G

G

I

L

E

E

I

L

E

E

G

R

E

E

A

C

S

K

R

D

V

Y

Q

V

K

L

K

K

A

A

L

L

Q

E

K

K

G

G

L

L

V

I

L

N

T

C

A

T

G

A

P

G

N

K

V

V

V

L

R

R

L

F

L

L

P

P

L

L

I

I

S

Q

K

K

E

E

E

H

L

I

E

D

A

R

G

A

L

I

N

S

T

V

L

L

Y

R

E

E

I

I

I

L

GT 96:97 (≠ GA 102:103) binding pyridoxal 5'-phosphate
K242 (= K248) modified: N6-(pyridoxal phosphate)lysine
T271 (= T277) binding pyridoxal 5'-phosphate

2eh6A Crystal structure of acetylornithine aminotransferase from aquifex aeolicus vf5
47% identity, 97% coverage: 10:387/390 of query aligns to 2:375/375 of 2eh6A

query
sites
2eh6A

Y

Y

L

L

M

M

N

N

T

N

Y

Y

N

A

H

R

L

L

P

P

V

V

V

K

F

F

T

V

H

R

G

G

E

K

G

G

C

V

K

Y

L

L

F

Y

D

D

T

E

D

E

N

G

K

K

E

E

Y

Y

L

L

D

D

F

F

T

V

S

S

G

G

I

I

G

G

V

V

M

N

S

S

L

L

G

G

Y

H

G

A

N

Y

K

P

N

K

W

L

I

T

K

E

A

A

V

L

E

K

A

E

Q

Q

L

V

E

E

K

K

V

L

H

H

T

V

S

S

N

N

I

L

F

Y

L

E

N

N

I

P

P

W

V

Q

L

E

E

E

L

L

A

A

K

H

K

K

F

L

T

V

E

K

-

H

I

F

S

W

N

T

M

E

T

G

K

K

V

V

F

F

C

A

N

N

S

S

G
|
G

A
x
T

E

E

A

S

N

V

E

E

G

A

A

A

I

I

K

K

L

L

A

A

R

R

K

K

Y

Y

S

W

F

R

D

D

K

K

H

-

G

G

K

K

A

N

R

K

N

W

T

K

I

F

L

I

T

S

L

F

K

E

K

N

S

S

F
|
F

H
|
H

G

G

R

R

T

T

I

Y

T

G

T

S

L

L

K

S

A

A

G

T

G

G

Q

Q

E

P

K

K

L

F

H

H

K

K

Y

G

F

F

Y

E

P

P

F

L

T

V

E

P

G

G

F

F

K

S

Y

Y

A

A

E

K

A

L

N

N

-

D

V

I

E

D

E

S

L

V

E

Y

K

K

S

L

I

L

D

D

S

E

I

T

C

A

A

G

I

I

M

I

I

I

E
|
E

P

V

I

I

Q

Q

G

G

E

E

G

G

I

V

N

N

P

E

L

A

S

S

E

E

E

D

F

F

V

L

H

S

K

K

V

L

F

Q

G

E

I

I

A

C

E

K

K

E

E

K

D

D

I

V

L

L

V

L

I

I

C

I

D
|
D

E

E

I
x
V

Q
|
Q

C

T

G

G

I

I

G

G

R

R

T

T

G

G

K

E

I

F

Y

Y

G

A

F

Y

N

Q

N

H

Y

F

G

N

V

L

C

K

P

P

D

D

I

V

I

I

S

A

T

L

A

A

K
|
K

G

G

L

L

G

G

L

V

P

P

I

I

G

G

A

A

V

I

L

L

C

A

N

R

E

E

K

E

L

V

N

A

N

Q

T

S

F

F

E

T

Y

P

G

G

D

S

H

H

G

G

S

S

T
|
T

F

F

G

G

N

N

P

P

V

L

-

A

C

C

A

R

A

A

G

G

A

T

L

V

E

V

V

V

L

D

N

E

I

V

I

-

S

-

E

-

N

E

S

K

F

L

L

L

E

P

E

H

V

V

S

R

E

E

K

V

G

G

K

N

F

Y

V

F

K

K

E

E

Y

K

F

L

K

K

S

E

K

L

N

G

K

K

K

G

N

-

I

-

L

-

E

K

V

V

R

K

G

G

M

R

G

G

L

L

M

M

I

L

G

G

I

L

E

E

I

L

E

E

G

R

E

E

A

C

S

K

R

D

V

Y

Q

V

K

L

K

K

A

A

L

L

Q

E

K

K

G

G

L

L

V

I

L

N

T

C

A

T

G

A

P

G

N

K

V

V

V

L

R
|
R

L

F

L

L

P

P

L

L

I

I

S

Q

K

K

E

E

E

H

L

I

E

D

A

R

G

A

L

I

N

S

T

V

L

L

Y

R

E

E

I

I

I

L

active site: F127 (= F135), E179 (= E186), D212 (= D219), Q215 (= Q222), K241 (= K248), T270 (= T277), R352 (= R364)
binding pyridoxal-5'-phosphate: G95 (= G102), T96 (≠ A103), F127 (= F135), H128 (= H136), E179 (= E186), D212 (= D219), V214 (≠ I221), K241 (= K248)

P73133 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Synechocystis sp. (strain PCC 6803 / Kazusa) (see paper)
43% identity, 97% coverage: 10:387/390 of query aligns to 34:425/429 of P73133

query
sites
P73133

Y

Y

L

V

M

M

N

N

T

T

Y
|
Y

N

G

H

R

L

F

P

P

V

I

V

A

F

I

T

A

H

R

G

G

E

Q

G

G

C

S

K

T

L

L

F

W

D

D

T

T

D

E

N

G

K

K

E

S

Y

Y

L

L

D

D

F

F

T

V

S

A

G

G

I

I

G

A

V

T

M

C

S

T

L

L

G

G

Y

H

G

A

N

H

K

P

N

A

W

L

I

V

K

R

A

A

V

V

E

S

A

D

Q

Q

L

I

E

Q

K

K

V

L

V

H

H

H

T

V

S

S

N

N

I

L

F

Y

L

Y

N

I

I

P

P

E

V

Q

L

G

E

E

L

L

A

A

K

K

K

W

F

I

T

V

E

E

I

H

S

S

N

C

M

A

T

D

K

R

V

V

F

F

C

C

N

N

S
|
S

G
|
G

A
|
A

E

E

A

A

N

N

E

E

G

A

A

A

I

I

K

K

L

L

A

V

R

R

K

K

Y

Y

S

A

F

H

D

T

K

V

H

L

G

D

-

F

K

L

A

E

R

Q

N

P

T

V

I

I

L

L

T

T

L

A

K

K

K

A

S

S

F

F

H

H

G

G

R
|
R

T

T

I

L

T

A

T

T

L

I

K

T

A

A

G

T

G

G

Q

Q

E

P

K

K

L

Y

H

Q

K

Q

Y

Y

F

F

Y

D

P

P

F

L

T

V

E

P

G

G

F

F

K

D

Y

Y

A

V

E

P

A

Y

N

N

-

D

V

I

E

R

E

S

L

L

E

E

K

N

S

K

I

V

-

A

-

D

-

L

-

D

-

E

-

G

D

N

S

S

S

R

I

V

C

A

A

A

I

I

M

F

I

L

E

E

P

P

I

L

Q

Q

G

G

E
|
E

G

G

I

V

N

R

P

P

L

G

S

D

E

L

E

A

F

Y

V

F

H

K

K

R

V

V

F

R

G

E

I

I

A

C

E

D

K

Q

E

N

D

D

I

I

L

L

V

L

I

V

C

F

D
|
D

E

E

I

V

Q
|
Q

C

V

G

G

I

V

G

G

R

R

T

T

G

G

K

K

I

L

Y

W

G

G

F

Y

N

E

N

H

Y

L

G

G

V

V

C

E

P

P

D

D

I

I

I

F

S

T

T

S

A

A

K
|
K

G

G

L

L

G

A

G

G

L

V

P

P

I

I

G

G

A

A

V

M

L

M

C

C

N

-

E

K

K

K

L

F

N

C

N

D

T

V

F

F

E

E

Y

P

G

G

D

N

H

H

G

A

S

S

T
|
T

F

F

G

G

N

N

P

P

V

L

C

A

A

C

A

A

G

A

A

G

L

L

E

A

V

V

L

L

N

K

I

T

I

I

S

E

E

G

N

D

S

R

F

L

L

L

E

D

E

N

V

V

S

Q

E

A

K

R

G

G

K

E

F

Q

V

L

K

R

E

S

Y

G

F

L

K

A

S

E

-

I

K

K

N

N

K

Q

K

Y

N

P

I

T

L

L

-

F

-

T

E

E

V

V

R

R

G

G

M

W

G

G

L

L

M

I

I

N

G

G

I

L

E

E

I

I

E

S

G

A

E

E

A

S

S

S

-

L

-

T

-

S

-

V

R

E

V

I

Q

V

K

K

K

A

A

A

L

M

Q

E

K

Q

G

G

L

L

V

L

L

A

T

P

A

A

G

G

P

P

N

K

V

V

V

L

R
|
R

L

F

L

V

P

P

L

L

I

V

S

T

K

E

E

A

E

E

L

I

E

A

A

Q

G

A

L

V

N

E

T

I

L

L

Y

R

E

Q

I

A

I

I

Y39 (= Y15) mutation to F: Retains 7.4% of N-acetylornithine aminotransferase wild-type activity.
S125 (= S101) mutation to A: Retains 14% of N-acetylornithine aminotransferase wild-type activity.
G126 (= G102) mutation to A: Loss of N-acetylornithine aminotransferase activity.
A127 (= A103) mutation to S: Retains 9.1% of N-acetylornithine aminotransferase wild-type activity.
R163 (= R138) mutation to A: 4100-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 9.5% of N-acetylornithine aminotransferase wild-type activity.
E223 (= E191) mutation to A: 65-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 50% of N-acetylornithine aminotransferase wild-type activity.; mutation to S: 73-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Shows 160% of N-acetylornithine aminotransferase wild-type activity.
D251 (= D219) mutation to A: Loss of N-acetylornithine aminotransferase activity.; mutation to E: Retains 20% of N-acetylornithine aminotransferase wild-type activity.
Q254 (= Q222) mutation to A: Retains 0.9% of N-acetylornithine aminotransferase wild-type activity.
K280 (= K248) mutation to A: Loss of N-acetylornithine aminotransferase activity.
T308 (= T277) mutation to A: Retains 0.3% of N-acetylornithine aminotransferase wild-type activity.
R402 (= R364) mutation to A: 2080-fold increase in KM for acetylornithine, but does not affect KM for 2-oxoglutarate. Retains 18% of N-acetylornithine aminotransferase wild-type activity.

4adbB Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
41% identity, 98% coverage: 9:389/390 of query aligns to 10:397/401 of 4adbB

query
sites
4adbB

E

E

Y

W

L

M

M

I

N

P

T

V

Y

Y

N

A

H

P

L

A

P

P

V

F

V

I

F

P

T

V

H

R

G

G

E

E

G

G

C

S

K

R

L

L

F

W

D

D

T

Q

D

Q

N

G

K

K

E

E

Y

Y

L

I

D

D

F

F

T

A

S

G

G

G

I

I

G

A

V

V

M

N

S

A

L

L

G

G

Y

H

G

A

N

H

K

P

N

E

W

L

I

R

K

E

A

A

V

L

E

N

A

E

Q

Q

L

A

E

S

K

K

V

F

V

W

H

H

T

T

S

G

N

N

I

G

F

Y

L

T

N

N

I

E

P

P

V

V

L

L

E

R

L

L

A

A

K

K

F

L

T

I

E

D

I

A

S

T

N

F

M

A

T

D

K

R

V

V

F

F

C

C

N

N

S
|
S

G
|
G

A
|
A

E

E

A

A

N

N

E

E

G

A

A

A

I

L

K

K

L

L

A

A

R

R

K

K

Y

F

S

A

F

H

D

D

K

R

H

Y

G

G

K

S

A

H

R

K

N

S

T

G

I

I

L

V

T

A

L

F

K

K

K

N

S

A

F
|
F

H
|
H

G

G

R

R

T

T

I

L

T

F

T

T

L

V

K

S

A

A

G

G

Q

Q

E

P

K

A

L

Y

H

S

K

Q

Y

D

F

F

Y

A

P

P

F

L

T

P

E

A

G

D

F

I

K

R

Y

H

A

A

E

A

A

Y

N

N

-

D

V

I

E

N

E

S

L

A

E

S

K

A

S

L

I

I

D

D

S

D

S

S

I

T

C

C

A

A

I

V

M

I

I

V

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

I

V

N

V

P

P

L

A

S

S

E

N

E

A

F

F

V

L

H

Q

K

G

V

L

F

R

G

E

I

L

A

C

E

N

K

R

E

H

D

N

I

A

L

L

V

L

I

I

C

F

D
|
D

E

E

I
x
V

Q
|
Q

C

T

G

G

I

V

G

G

R

R

T

T

G

G

K

E

I

L

Y

Y

G

A

F

Y

N

M

N

H

Y

Y

G

G

V

V

C

T

P

P

D

D

I

L

S

T

T

T

A

A

K
|
K

G

A

L

L

G

G

L

F

P

P

I

V

G

G

A

A

V

L

L

L

C

A

N

T

E

E

K

E

L

C

N

A

N

R

T

V

F

M

E

T

Y

V

G

G

D

T

H

H

G

G

S

T

T
|
T

F

Y

G

G

N

N

P

P

V

L

C

A

A

S

A

A

G

V

A

A

L

G

E

K

V

V

L

L

N

E

I

L

I

I

S

N

E

T

N

P

S

E

F

M

L

L

E

N

E

G

V

V

S

K

E

Q

K

R

G

H

K

D

F

W

V

F

K

V

E

E

Y

R

F

L

K

N

S

T

K

I

N

N

K

H

K

R

N

Y

I

G

L

L

-

F

-

S

E

E

V

V

R

R

G

G

M

L

G

G

L

L

M

L

I

I

G

G

-

C

-

V

-

L

-

N

I

A

E

D

I

Y

E

A

G

G

E

Q

A

A

S

K

R

Q

V

I

Q

S

K

Q

K

E

A

A

L

A

Q

K

K

A

G

G

L

V

L

M

V

V

L

L

T

I

A

A

G

G

P

G

N

N

V

V

R
|
R

L

F

L

A

P

P

P

A

L

L

I

N

I

V

S

S

K

E

E

E

L

V

E

T

A

T

G

G

L

L

N

D

T

R

L

F

Y

A

E

A

I

A

I

C

E

E

E

H

active site: F136 (= F135), E188 (= E186), D221 (= D219), Q224 (= Q222), K250 (= K248), T279 (= T277), R372 (= R364)
binding pyridoxal-5'-phosphate: S102 (= S101), G103 (= G102), A104 (= A103), F136 (= F135), H137 (= H136), D221 (= D219), V223 (≠ I221), Q224 (= Q222), K250 (= K248)

4addA Structural and functional study of succinyl-ornithine transaminase from e. Coli (see paper)
41% identity, 98% coverage: 9:389/390 of query aligns to 10:397/400 of 4addA

query
sites
4addA

E

E

Y

W

L

M

M

I

N

P

T

V

Y
|
Y

N

A

H

P

L

A

P

P

V

F

V

I

F

P

T

V

H

R

G

G

E

E

G

G

C

S

K

R

L

L

F

W

D

D

T

Q

D

Q

N

G

K

K

E

E

Y

Y

L

I

D

D

F

F

T

A

S

G

G

G

I

I

G

A

V

V

M

N

S

A

L

L

G

G

Y

H

G

A

N

H

K

P

N

E

W

L

I

R

K

E

A

A

V

L

E

N

A

E

Q

Q

L

A

E

S

K

K

V

F

V

W

H

H

T

T

S

G

N

N

I

G

F

Y

L

T

N

N

I

E

P

P

V

V

L

L

E

R

L

L

A

A

K

K

F

L

T

I

E

D

I

A

S

T

N

F

M

A

T

D

K

R

V

V

F

F

C

C

N

N

S

S

G
|
G

A
|
A

E

E

A

A

N

N

E

E

G

A

A

A

I

L

K

K

L

L

A

A

R

R

K

K

Y

F

S

A

F

H

D

D

K

R

H

Y

G

G

K

S

A

H

R

K

N

S

T

G

I

I

L

V

T

A

L

F

K

K

K

N

S

A

F
|
F

H
|
H

G

G

R
|
R

T

T

I

L

T

F

T

T

L

V

K

S

A

A

G

G

Q

Q

E

P

K

A

L

Y

H

S

K

Q

Y

D

F

F

Y

A

P

P

F

L

T

P

E

A

G

D

F

I

K

R

Y

H

A

A

E

A

A

Y

N

N

-

D

V

I

E

N

E

S

L

A

E

S

K

A

S

L

I

I

D

D

S

D

S

S

I

T

C

C

A

A

I

V

M

I

I

V

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

I

V

N

V

P

P

L

A

S

S

E

N

E

A

F

F

V

L

H

Q

K

G

V

L

F

R

G

E

I

L

A

C

E

N

K

R

E

H

D

N

I

A

L

L

V

L

I

I

C

F

D
|
D

E

E

I
x
V

Q
|
Q

C

T

G

G

I

V

G

G

R

R

T

T

G

G

K

E

I

L

Y

Y

G

A

F

Y

N

M

N

H

Y

Y

G

G

V

V

C

T

P

P

D

D

I

L

S

T

T

T

A

A

K
|
K

G

A

L

L

G

G

L

F

P

P

I

V

G

G

A

A

V

L

L

L

C

A

N

T

E

E

K

E

L

C

N

A

N

R

T

V

F

M

E

T

Y

V

G

G

D

T

H

H

G

G

S

T

T
|
T

F

Y

G

G

N

N

P

P

V

L

C

A

A

S

A

A

G

V

A

A

L

G

E

K

V

V

L

L

N

E

I

L

I

I

S

N

E

T

N

P

S

E

F

M

L

L

E

N

E

G

V

V

S

K

E

Q

K

R

G

H

K

D

F

W

V

F

K

V

E

E

Y

R

F

L

K

N

S

T

K

I

N

N

K

H

K

R

N

Y

I

G

L

L

-

F

-

S

E

E

V

V

R

R

G

G

M

L

G

G

L

L

M

L

I

I

G

G

-

C

-

V

-

L

-

N

I

A

E

D

I

Y

E

A

G

G

E

Q

A

A

S

K

R

Q

V

I

Q

S

K

Q

K

E

A

A

L

A

Q

K

K

A

G

G

L

V

L

M

V

V

L

L

T

I

A

A

G

G

P

G

N

N

V

V

R
|
R

L

F

L

A

P

P

P

A

L

L

I

N

I

V

S

S

K

E

E

E

L

V

E

T

A

T

G

G

L

L

N

D

T

R

L

F

Y

A

E

A

I

A

I

C

E

E

E

H

active site: F136 (= F135), E188 (= E186), D221 (= D219), Q224 (= Q222), K250 (= K248), T279 (= T277), R372 (= R364)
binding pyridoxal-5'-phosphate: G103 (= G102), A104 (= A103), F136 (= F135), H137 (= H136), D221 (= D219), V223 (≠ I221), K250 (= K248)
binding n~2~-(3-carboxypropanoyl)-l-ornithine: Y16 (= Y15), F136 (= F135), R139 (= R138)

3nx3A Crystal structure of acetylornithine aminotransferase (argd) from campylobacter jejuni
40% identity, 95% coverage: 8:377/390 of query aligns to 1:375/388 of 3nx3A

query
sites
3nx3A

K

Q

E

S

Y

H

L

I

M

I

N

P

T

T

Y

Y

N

K

H

R

L

F

P

D

V

I

V

V

F

L

T

E

H

K

G

G

E

Q

G

G

C

V

K

Y

L

L

F

F

D

D

T

D

D

K

N

A

K

K

E

K

Y

Y

L

L

D

D

F

F

T

S

S

S

G

G

I

I

G

G

V

V

M

C

S

A

L

L

G

G

Y

Y

G

N

N

H

K

A

N

K

W

F

I

N

K

A

A

K

V

I

E

K

A

A

Q

Q

L

V

E

D

K

K

V

L

H

H

T

T

S

S

N

N

I

L

F

Y

L

Y

N

N

I

E

P

N

V

I

L

A

E

A

L

A

A

A

K

K

K

N

F

L

T

A

E

K

I

A

S

S

N

A

M

L

T

E

K

R

V

V

F

F

C

T

N

N

S

S

G

G

A

T

E

E

A

S

N

I

E

E

G

G

A

A

I

M

K

K

L

T

A

A

R

R

K

K

Y

Y

S

A

F

F

D

N

K

K

H

-

G

G

K

V

A

K

R

G

N

G

T

Q

I

F

L

I

T

A

L

F

K

K

K

H

S

S

F
|
F

H

H

G

G

R

R

T

T

I

L

T

G

T

A

L

L

K

S

A

L

G

T

G

A

Q

N

E

E

K

K

L

Y

H

Q

K

K

Y

P

F

F

Y

K

P

P

F

L

T

I

E

S

G

G

F

V

K

K

Y

F

A

A

E

K

A

Y

N

N

-

D

V

I

E

S

L

V

E

E

K

K

S

L

I

V

D

N

S

E

S

K

I

T

C

C

A

A

I

I

M

I

I

L

E
|
E

P

S

I

V

Q

Q

G

G

E

E

G

G

I

I

N

N

P

P

L

A

S
x
N

E

K

E

D

F
|
F

V

Y

H

K

K

A

V

L

F

R

G

K

I

L

A

C

E

D

K

E

E

K

D

D

I

I

L

L

V

L

I

I

C

A

D
|
D

E

E

I

I

Q
|
Q

C

C

G

G

I

M

G

G

R

R

T

S

G

G

K

K

I

F

Y

F

G

A

F

Y

N

E

N

H

Y

A

G

Q

V

I

C

L

P

P

D

D

I

I

I

M

S

T

T

S

A

A

K
|
K

G

A

L

L

G

G

G

C

G

G

L

L

P

S

I

V

G

G

A

A

V

F

L

V

C

I

N

N

E

Q

K

K

L

V

-

A

N

S

N

N

T

S

F

L

E

E

Y

A

G

G

D

D

H

H

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

V

L

C

V

A

C

A

A

G

G

A

V

L

N

E

A

V

V

L

F

N

E

I

I

I

F

S

K

E

E

N

E

S

K

F

I

L

L

E

E

E

N

V

V

S

N

E

K

K

L

G

T

K

P

F

Y

V

L

K

E

E

Q

Y

S

F

L

K

D

S

E

K

L

-
x
I

N

N

K

E

K
x
F

N
x
D

I

F

L
x
C

E

K

V

K

R

R

-

K

G

G

M

L

G

G

L

F

M

M

I

Q

G

G

I

L

E

S

I

L

E

D

G

K

-

S

-

V

E

K

A

V

S

A

R

K

V

V

Q

I

K

Q

K

K

A

C

L

Q

Q

E

K

N

G

A

L

L

V

L

L

I

T

S

A

C

G

G

P

E

N

N

V

D

V

L

R
|
R

L

F

L

L

P

P

L

L

I

I

I

L

S
x
Q

K
|
K

E

E

E

H

L

I

E

D

active site: F127 (= F135), E179 (= E186), D212 (= D219), Q215 (= Q222), K241 (= K248), T271 (= T277), R362 (= R364)
binding magnesium ion: N191 (≠ S198), F194 (= F201), I313 (vs. gap), F316 (≠ K321), D317 (≠ N322), C319 (≠ L324), Q370 (≠ S372), K371 (= K373)

P40732 Acetylornithine/succinyldiaminopimelate aminotransferase; ACOAT; DapATase; Succinyldiaminopimelate transferase; EC 2.6.1.11; EC 2.6.1.17 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
34% identity, 97% coverage: 9:387/390 of query aligns to 15:400/405 of P40732

query
sites
P40732

E

E

Y

V

L

I

M

L

N

P

T

V

Y

Y

N

A

H

P

L

A

P

D

V

F

V

I

F

P

T

V

H

K

G

G

E

K

G

G

C

S

K

R

L

V

F

W

D

D

T

Q

D

Q

N

G

K

K

E

E

Y

Y

L

I

D

D

F

F

T

A

S

G

G

G

I

I

G

A

V

V

M

T

S

A

L

L

G

G

Y

H

G

C

N

H

K

P

N

A

W

L

I

V

K

E

A

A

V

L

E

K

A

S

Q

Q

L

G

E

E

K

T

V

L

V

W

H

H

T

T

S

S

N

N

I

V

F

F

L

T

N

N

I

E

P

P

V

A

L

L

E

R

L

L

A

G

K

R

K

K

F

L

T

I

E

D

I

A

S

T

N

F

M

A

T

E

K

R

V

V

F

L

F

F

C

M

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

T

A

A

I

F

K

K

L

L

A

A

R

R

K

H

Y

Y

S

A

F

C

D

V

K

R

H

H

G

S

K

P

A

F

R

K

N

T

T

K

I

I

L

I

T

A

L

F

K

H

K

N

S

A

F

F

H

H

G

G

R

R

T

S

I

L

T

F

T

T

L

V

K

S

A

V

G

G

Q

Q

E

P

K

K

L

Y

H

S

K

D

Y

G

F

F

Y

G

P

P

F

K

T

P

E

A

G

D

F

I

K

I

Y

H

A

V

E

P

A

F

N

N

-

D

V

L

E

H

E

A

L

V

E

K

K

A

S

V

I

M

D

D

S

D

S

H

I

T

C

C

A

A

I

V

M

V

I

V

E

E

P

P

I

I

Q

Q

G

G

E

E

G

G

I

V

N

Q

P

A

L

A

S

T

E

P

E

E

F

F

V

L

H

K

K

G

V

L

F

R

G

D

I

L

A

C

E

D

K

E

E

H

D

Q

I

A

L

L

V

L

I

V

C

F

D

D

E

E

I

V

Q

Q

C

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

I

L

Y

F

G

A

F

Y

N

M

N

H

Y

Y

G

G

V

V

C

T

P

P

D

D

I

I

I

L

S

T

T

S

A

A

K
|
K

G

A

L

L

G

G

L

F

P

P

I

V

G

S

A

A

V

M

L

L

C

T

N

T

E

Q

K

E

L

I

N

A

N

S

T

A

F

F

E

H

Y

V

G

G

D

S

H

H

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

V

L

C

A

A

C

A

A

G

V

A

A

L

G

E

A

V

A

L

F

N

D

I

I

S

N

E

T

N

P

S

E

F

V

L

L

E

Q

E

G

V

I

-

H

S

T

E

K

K

R

G

Q

K

Q

F

F

V

V

K

Q

E

H

Y

L

F

Q

K

A

S

I

K

D

N

E

K

Q

K

F

N

D

I

I

L

F

-

S

E

D

V

I

R

R

G

G

M

M

G

G

L

L

M

L

I

I

G

G

I

A

E

E

I

L

-

K

-

P

-

K

-

Y

E

K

G

G

E

R

A

A

S

R

R

D

V

F

Q

L

K

Y

K

A

A

G

L

A

Q

E

K

A

G

G

L

V

L

M

V

V

L

L

T

N

A

A

G

G

P

A

N

D

V

V

V

M

R

R

L

F

L

A

P

P

P

S

L

L

I

V

S

E

K

E

E

A

E

D

L

I

E

H

A

E

G

G

L

M

N

Q

T

R

L

F

Y

A

E

Q

I

A

I

V

GT 108:109 (≠ GA 102:103) binding pyridoxal 5'-phosphate
K255 (= K248) modified: N6-(pyridoxal phosphate)lysine
T284 (= T277) binding pyridoxal 5'-phosphate

4jevB N-acetylornithine aminotransferase from s. Typhimurium complexed with gabaculine
34% identity, 97% coverage: 9:387/390 of query aligns to 10:395/402 of 4jevB

query
sites
4jevB

E

E

Y

V

L

I

M

L

N

P

T

V

Y

Y

N

A

H

P

L

A

P

D

V

F

V

I

F

P

T

V

H

K

G

G

E

K

G

G

C

S

K

R

L

V

F

W

D

D

T

Q

D

Q

N

G

K

K

E

E

Y

Y

L

I

D

D

F

F

T

A

S

G

G

G

I
|
I

G

A

V

V

M

T

S

A

L

L

G

G

Y

H

G

C

N

H

K

P

N

A

W

L

I

V

K

E

A

A

V

L

E

K

A

S

Q

Q

L

G

E

E

K

T

V

L

V

W

H

H

T

T

S

S

N

N

I

V

F

F

L

T

N

N

I

E

P

P

V

A

L

L

E

R

L

L

A

G

K

R

K

K

F

L

T

I

E

D

I

A

S

T

N

F

M

A

T

E

K

R

V

V

F

L

F

F

C

M

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

T

A

A

I

F

K

K

L

L

A

A

R

R

K

H

Y

Y

S

A

F

C

D

V

K

R

H

H

G

S

K

P

A

F

R

K

N

T

T

K

I

I

L

I

T

A

L

F

K

H

K

N

S

A

F
|
F

H
|
H

G

G

R

R

T

S

I

L

T

F

T

T

L

V

K

S

A

V

G

G

Q

Q

E

P

K

K

L

Y

H

S

K

D

Y

G

F

F

Y

G

P

P

F

K

T

P

E

A

G

D

F

I

K

I

Y

H

A

V

E

P

A

F

N

N

-

D

V

L

E

H

E

A

L

V

E

K

K

A

S

V

I

M

D

D

S

D

S

H

I

T

C

C

A

A

I

V

M

V

I

V

E
|
E

P

P

I

I

Q

Q

G

G

E
|
E

G

G

I

V

N

Q

P

A

L

A

S

T

E

P

E

E

F

F

V

L

H

K

K

G

V

L

F

R

G

D

I

L

A

C

E

D

K

E

E

H

D

Q

I

A

L

L

V

L

I

V

C

F

D
|
D

E

E

I
x
V

Q
|
Q

C

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

I

L

Y

F

G

A

F

Y

N

M

N

H

Y

Y

G

G

V

V

C

T

P

P

D

D

I

I

I

L

S

T

T

S

A

A

K
|
K

G

A

L

L

G

G

L

F

P

P

I

V

G

S

A

A

V

M

L

L

C

T

N

T

E

Q

K

E

L

I

N

A

N

S

T

A

F

F

E

H

Y

V

G

G

D

S

H

H

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

V

L

C

A

A

C

A

A

G

V

A

A

L

G

E

A

V

T

L

F

N

D

I

I

S

N

E

T

N

P

S

E

F

V

L

L

E

Q

E

G

V

I

-

H

S

T

E

K

K

R

G

Q

K

Q

F

F

V

V

K

Q

E

H

Y

L

F

Q

K

A

S

I

K

D

N

E

K

Q

K

F

N

D

I

I

L

F

-

S

E

D

V

I

R

R

G

G

M

M

G

G

L

L

M

L

I

I

G

G

I

A

E

E

I

L

-

K

-

P

-

K

-

Y

E

K

G

G

E

R

A

A

S

R

R

D

V

F

Q

L

K

Y

K

A

A

G

L

A

Q

E

K

A

G

G

L

V

L

M

V

V

L

L

T

N

A

A

G

G

P

A

N

D

V

V

V

M

R
|
R

L

F

L

A

P

P

P

S

L

L

I

V

S

E

K

E

E

A

E

D

L

I

E

H

A

E

G

G

L

M

N

Q

T

R

L

F

Y

A

E

Q

I

A

I

V

active site: F136 (= F135), E188 (= E186), D221 (= D219), Q224 (= Q222), K250 (= K248), T279 (= T277), R372 (= R364)
binding 3-[o-phosphonopyridoxyl]--amino-benzoic acid: I46 (= I45), S102 (= S101), G103 (= G102), T104 (≠ A103), F136 (= F135), H137 (= H136), E188 (= E186), E193 (= E191), D221 (= D219), V223 (≠ I221), Q224 (= Q222), K250 (= K248), R372 (= R364)

8ht4B Crystal structure of acetylornithine aminotransferase complex with plp from corynebacterium glutamicum (see paper)
37% identity, 98% coverage: 4:387/390 of query aligns to 3:389/390 of 8ht4B

query
sites
8ht4B

L

L

N

E

Y

T

A

W

K

P

E

Q

Y

V

L

I

M

I

N

N

T

T

Y

Y

N

G

H

T

L

P

P

P

V

V

V

E

F

L

T

V

H

S

G

G

E

K

G

G

C

A

K

T

L

V

F

T

D

D

T

D

D

Q

N

G

K

N

E

V

Y

Y

L

I

D

D

F

L

T

L

S

A

G

G

I

I

G

A

V

V

M

N

S

A

L

L

G

G

Y

H

G

A

N

H

K

P

N

A

W

I

I

I

K

E

A

A

V

V

E

T

A

N

Q

Q

L

I

E

G

K

Q

V

L

V

G

H

H

T

V

S

S

N

N

I

L

F

F

L

A

N

S

I

R

P

P

V

V

L

V

E

E

L

V

A

A

-

E

-

L

-

I

K

K

K

R

F

F

T

S

-

L

-

D

-

A

E

T

I

L

S

A

N

A

M

Q

T

T

K

R

V

V

F

F

C

C

N

N

S
|
S

G
|
G

A
|
A

E

E

A

A

N

N

E

E

G

A

A

A

I

F

K

K

L

I

A

A

R

R

K

L

Y

-

S

-

F

-

D

-

K

-

H

-

G

-

K

T

A

G

R

R

N

S

T

R

I

I

L

L

T

A

L

A

K

V

K

H

S

G

F
|
F

H
|
H

G

G

R

R

T

T

I

M

T

G

T

S

L

L

K

A

A

L

G

T

G

G

Q

Q

E

P

K

D

L

K

H

R

K

E

Y

A

F

F

Y

L

P

P

F

M

T

P

E

S

G

G

F

V

K

E

-

F

Y

Y

A

P

E

Y

A

G

N

D

V

T

E

D

E

Y

L

L

E

R

K

K

S

M

I

V

D

E

S

T

S

N

-

P

-

T

-

D

I

V

C

A

A

A

I

I

M

F

I

L

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

T

G

G

I

V

N

V

P

P

L

A

S

P

E

E

E

G

F

F

V

L

H

K

K

A

V

V

F

R

G

E

I

L

A

C

E

D

K

E

E

Y

D

G

I

I

L

L

V

M

I

I

C

T

D
|
D

E

E

I
x
V

Q
|
Q

C

T

G

G

I

V

G

G

R

R

T

T

G

G

K

D

I

F

Y

F

G

A

F

H

N

Q

N

H

Y

D

G

G

V

V

C

V

P

P

D

D

I

V

S

T

T

M

A

A

K
|
K

G

G

L

L

G

G

L

L

P

P

I

I

G

G

A

A

V

C

L

L

C

A

N

T

E

G

K

R

L

A

N

A

N

E

T

L

F

M

E

T

Y

P

G

G

D

K

H

H

G

G

S

T

T

T

F

F

G

G

N

N

P

P

V

V

C

A

A

C

A

A

G

A

A

A

L

K

E

A

V

V

L

L

N

S

I

V

S

-

E

D

N

D

S

A

F

F

L

C

E

A

E

E

V

V

S

A

E

R

K

K

G

G

K

E

F

L

V

F

K

K

E

E

Y

L

F

L

K

A

S

K

K

V

N

D

K

-

N

G

I

V

L

V

E

D

V

V

R

R

G

G

M

R

G

G

L

L

M

M

I

L

G

G

I

V

E

V

I

L

E

E

G

R

E

D

-

V

A

A

S

K

R

Q

V

A

Q

V

K

L

K

D

A

G

L

F

Q

K

K

H

G

G

L

V

L

I

V

L

L

N

T

A

A

P

G

A

P

D

N

N

V

I

R

R

L

L

L

T

P

P

L

L

I

V

I

I

S

T

K

D

E

E

L

I

E

A

A

D

G

A

L

V

N

K

T

A

L

I

Y

A

E

E

I

T

I

I

binding pyridoxal-5'-phosphate: S108 (= S101), G109 (= G102), A110 (= A103), F135 (= F135), H136 (= H136), E190 (= E186), D223 (= D219), V225 (≠ I221), Q226 (= Q222), K252 (= K248)

Q5SHH5 [LysW]-aminoadipate semialdehyde transaminase; EC 2.6.1.118 from Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8)
36% identity, 93% coverage: 15:377/390 of query aligns to 24:384/395 of Q5SHH5

query
sites
Q5SHH5

Y

Y

N

N

H

K

L

H

P

D

V

L

F

I

T

V

H

R

G

G

E

Q

G

G

C

A

K

R

L

V

F

W

D

D

T

A

D

E

N

G

K

N

E

E

Y

Y

L

I

D

D

F

C

T

V

S

G

G

G

I

Y

G

G

V

V

M

A

S

N

L

L

G

G

Y

H

G

G

N

N

K

P

N

E

W

V

I

V

K

E

A

A

V

V

E

K

A

R

Q

Q

L

A

E

E

K

T

V

L

V

M

H

A

T

M

S

P

N

Q

I

T

F

L

L

P

N

T

I

P

P

M

V

R

L

G

E

E

L

F

A

Y

K

R

K

T

F

L

T

T

E

A

I

I

-

L

-

P

S

P

N

E

M

L

T

N

K

R

V

V

F

F

F

P

C

V

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

A

A

A

I

L

K

K

L

F

A

A

R

R

K

A

Y

H

S

T

F

-

D

-

K

-

H

-

G

-

K

-

A

G

R

R

N

K

T

K

I

F

L

V

T

A

L

A

K

M

K

R

S

G

F

F

H

S

G

G

R

R

T

T

I

M

T

G

T

S

L

L

K

S

A

V

G

T

G

W

Q

E

E

P

K

K

L

Y

H

R

K

E

Y

P

F

F

Y

L

P

P

F

L

T

V

E

E

G

P

F

V

K

E

Y

F

A

I

E

P

A

Y

N

N

-

D

V

V

E

E

E

A

L

L

E

K

K

R

S

A

I

V

D

D

S

E

I

T

C

A

A

A

I

V

M

I

I

L

E

E

P

P

I

V

Q

Q

G

G

E

E

G

G

I

V

N

R

P

P

L

A

S

T

E

P

E

E

F

F

V

L

H

R

K

A

V

A

F

R

G

E

I

I

A

T

E

Q

K

E

E

K

D

G

I

A

L

L

V

L

I

I

C

L

D

D

E

E

I

I

Q

Q

C

T

G

G

I

M

G

G

R

R

T

T

G

G

K

K

I

R

Y

F

G

A

F

F

N

E

N

H

Y

F

G

G

V

I

C

V

P

P

D

D

I

I

I

L

S

T

T

L

A

A

K
|
K

G

A

L

L

G

G

L

V

P

P

I

L

G

G

A

A

V

A

L

V

C

M

N

R

E

E

K

E

L

V

N

A

N

R

T

S

F

M

E

P

Y

K

G

G

D

G

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

V

L

C

A

A

M

A

A

G

A

A

G

L

V

E

A

V

A

L

I

N

R

I

Y

I

L

S

E

E

R

N

T

S

R

F

L

L

W

E

E

E

R

V

A

S

A

E

E

K

L

G

G

K

P

F

W

V

F

K

M

E

E

Y

K

F

L

K

R

S

A

K

I

N

P

K

S

K

P

N

K

I

I

L

R

E

E

V

V

R

R

G

G

M

M

G

G

L

L

M

M

I

V

G

G

I

L

E

E

I

L

-

K

E

E

G

K

E

A

A

A

S

P

R

Y

V

I

Q

A

K

R

K

L

A

E

L

K

Q

E

K

H

G

R

L

V

L

L

V

A

L

L

T

Q

A

A

G

G

P

P

N

T

V

V

V

I

R

R

L

F

L

L

P

P

L

L

I

V

I

I

S

E

K

K

E

E

E

D

L

L

E

E

GT 113:114 (≠ GA 102:103) binding pyridoxal 5'-phosphate
K254 (= K248) modified: N6-(pyridoxal phosphate)lysine
T283 (= T277) binding pyridoxal 5'-phosphate

1wkhA Acetylornithine aminotransferase from thermus thermophilus hb8
36% identity, 93% coverage: 15:377/390 of query aligns to 16:376/387 of 1wkhA

query
sites
1wkhA

Y

Y

N

N

H

K

L

H

P

D

V

L

F

I

T

V

H

R

G

G

E

Q

G

G

C

A

K

R

L

V

F

W

D

D

T

A

D

E

N

G

K

N

E

E

Y

Y

L

I

D

D

F

C

T

V

S

G

G

G

I
x
Y

G

G

V

V

M

A

S

N

L

L

G

G

Y

H

G

G

N

N

K

P

N

E

W

V

I

V

K

E

A

A

V

V

E

K

A

R

Q

Q

L

A

E

E

K

T

V

L

V

M

H

A

T

M

S

P

N

Q

I

T

F

L

L

P

N

T

I

P

P

M

V

R

L

G

E

E

L

F

A

Y

K

R

K

T

F

L

T

T

E

A

I

I

-

L

-

P

S

P

N

E

M

L

T

N

K

R

V

V

F

F

F

P

C

V

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

A

A

A

I

L

K

K

L

F

A

A

R

R

K

A

Y

H

S

T

F

-

D

-

K

-

H

-

G

-

K

-

A

G

R

R

N

K

T

K

I

F

L

V

T

A

L

A

K

M

K

R

S

G

F
|
F

H
x
S

G

G

R

R

T

T

I

M

T

G

T

S

L

L

K

S

A

V

G

T

G

W

Q

E

E

P

K

K

L

Y

H

R

K

E

Y

P

F

F

Y

L

P

P

F

L

T

V

E

E

G

P

F

V

K

E

Y

F

A

I

E

P

A

Y

N

N

-

D

V

V

E

E

E

A

L

L

E

K

K

R

S

A

I

V

D

D

S

E

I

T

C

A

A

A

I

V

M

I

I

L

E
|
E

P

P

I

V

Q

Q

G

G

E
|
E

G

G

I

V

N

R

P

P

L

A

S

T

E

P

E

E

F

F

V

L

H

R

K

A

V

A

F

R

G

E

I

I

A

T

E

Q

K

E

E

K

D

G

I

A

L

L

V

L

I

I

C

L

D
|
D

E

E

I
|
I

Q
|
Q

C

T

G

G

I

M

G

G

R

R

T

T

G

G

K

K

I

R

Y

F

G

A

F

F

N

E

N

H

Y

F

G

G

V

I

C

V

P

P

D

D

I

I

I

L

S

T

T

L

A

A

K
|
K

G

A

L

L

G

G

L

V

P

P

I

L

G

G

A

V

V

A

L

V

C

M

N

R

E

E

K

E

L

V

N

A

N

R

T

S

F

M

E

P

Y

K

G

G

D

G

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

V

L

C

A

A

M

A

A

G

A

A

G

L

V

E

A

V

A

L

I

N

R

I

Y

I

L

S

E

E

R

N

T

S

R

F

L

L

W

E

E

E

R

V

A

S

A

E

E

K

L

G

G

K

P

F

W

V

F

K

M

E

E

Y

K

F

L

K

R

S

A

K

I

N

P

K

S

K

P

N

K

I

I

L

R

E

E

V

V

R

R

G

G

M

M

G

G

L

L

M

M

I

V

G

G

I

L

E

E

I

L

-

K

E

E

G

K

E

A

A

A

S

P

R

Y

V

I

Q

A

K

R

K

L

A

E

L

K

Q

E

K

H

G

R

L

V

L

L

V

A

L

L

T

Q

A

A

G

G

P

P

N

T

V

V

V

I

R
|
R

L

F

L

L

P

P

L

L

I

V

I

I

S

E

K

K

E

E

E

D

L

L

E

E

active site: F132 (= F135), E184 (= E186), D217 (= D219), Q220 (= Q222), K246 (= K248), T275 (= T277), R363 (= R364)
binding 4-[(1,3-dicarboxy-propylamino)-methyl]-3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridinium: Y46 (≠ I45), S104 (= S101), G105 (= G102), T106 (≠ A103), F132 (= F135), S133 (≠ H136), E184 (= E186), E189 (= E191), D217 (= D219), I219 (= I221), K246 (= K248), R363 (= R364)

Sites not aligning to the query:

active site: 13

1wkgA Acetylornithine aminotransferase from thermus thermophilus hb8
36% identity, 93% coverage: 15:377/390 of query aligns to 16:376/387 of 1wkgA

query
sites
1wkgA

Y
|
Y

N

N

H

K

L

H

P

D

V

L

F

I

T

V

H

R

G

G

E

Q

G

G

C

A

K

R

L

V

F

W

D

D

T

A

D

E

N

G

K

N

E

E

Y

Y

L

I

D

D

F

C

T

V

S

G

G

G

I
x
Y

G

G

V

V

M

A

S

N

L

L

G

G

Y

H

G

G

N

N

K

P

N

E

W

V

I

V

K

E

A

A

V

V

E

K

A

R

Q

Q

L

A

E

E

K

T

V

L

V

M

H

A

T

M

S

P

N

Q

I

T

F

L

L

P

N

T

I

P

P

M

V

R

L

G

E

E

L

F

A

Y

K

R

K

T

F

L

T

T

E

A

I

I

-

L

-

P

S

P

N

E

M

L

T

N

K

R

V

V

F

F

F

P

C

V

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

A

A

A

I

L

K

K

L

F

A

A

R

R

K

A

Y

H

S

T

F

-

D

-

K

-

H

-

G

-

K

-

A

G

R

R

N

K

T

K

I

F

L

V

T

A

L

A

K

M

K

R

S

G

F
|
F

H
x
S

G

G

R
|
R

T

T

I

M

T

G

T

S

L

L

K

S

A

V

G

T

G

W

Q

E

E

P

K

K

L

Y

H

R

K

E

Y

P

F

F

Y

L

P

P

F

L

T

V

E

E

G

P

F

V

K

E

Y

F

A

I

E

P

A

Y

N

N

-

D

V

V

E

E

E

A

L

L

E

K

K

R

S

A

I

V

D

D

S

E

I

T

C

A

A

A

I

V

M

I

I

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

G

G

I

V

N

R

P

P

L

A

S

T

E

P

E

E

F

F

V

L

H

R

K

A

V

A

F

R

G

E

I

I

A

T

E

Q

K

E

E

K

D

G

I

A

L

L

V

L

I

I

C

L

D
|
D

E

E

I
|
I

Q
|
Q

C

T

G

G

I

M

G

G

R

R

T

T

G

G

K

K

I

R

Y

F

G

A

F

F

N

E

N

H

Y

F

G

G

V

I

C

V

P

P

D

D

I

I

I

L

S

T

T

L

A

A

K
|
K

G

A

L

L

G

G

L

V

P

P

I

L

G

G

A

V

V

A

L

V

C

M

N

R

E

E

K

E

L

V

N

A

N

R

T

S

F

M

E

P

Y

K

G

G

D

G

H

H

G
|
G

S
x
T

T
|
T

F

F

G

G

N

N

P

P

V

L

C

A

A

M

A

A

G

A

A

G

L

V

E

A

V

A

L

I

N

R

I

Y

I

L

S

E

E

R

N

T

S

R

F

L

L

W

E

E

E

R

V

A

S

A

E

E

K

L

G

G

K

P

F

W

V

F

K

M

E

E

Y

K

F

L

K

R

S

A

K

I

N

P

K

S

K

P

N

K

I

I

L

R

E

E

V

V

R

R

G

G

M

M

G

G

L

L

M

M

I

V

G

G

I

L

E

E

I

L

-

K

E

E

G

K

E

A

A

A

S

P

R

Y

V

I

Q

A

K

R

K

L

A

E

L

K

Q

E

K

H

G

R

L

V

L

L

V

A

L

L

T

Q

A

A

G

G

P

P

N

T

V

V

V

I

R
|
R

L

F

L

L

P

P

L

L

I

V

I

I

S

E

K

K

E

E

E

D

L

L

E

E

active site: F132 (= F135), E184 (= E186), D217 (= D219), Q220 (= Q222), K246 (= K248), T275 (= T277), R363 (= R364)
binding n~2~-acetyl-n~5~-({3-hydroxy-2-methyl-5-[(phosphonooxy)methyl]pyridin-4-yl}methyl)-l-ornithine: Y16 (= Y15), Y46 (≠ I45), G105 (= G102), T106 (≠ A103), F132 (= F135), S133 (≠ H136), R135 (= R138), E184 (= E186), D217 (= D219), I219 (= I221), Q220 (= Q222), K246 (= K248), G273 (= G275), T274 (≠ S276), T275 (= T277)

Sites not aligning to the query:

active site: 13

1vefA Acetylornithine aminotransferase from thermus thermophilus hb8
36% identity, 93% coverage: 15:377/390 of query aligns to 16:376/387 of 1vefA

query
sites
1vefA

Y

Y

N

N

H

K

L

H

P

D

V

L

F

I

T

V

H

R

G

G

E

Q

G

G

C

A

K

R

L

V

F

W

D

D

T

A

D

E

N

G

K

N

E

E

Y

Y

L

I

D

D

F

C

T

V

S

G

G

G

I

Y

G

G

V

V

M

A

S

N

L

L

G

G

Y

H

G

G

N

N

K

P

N

E

W

V

I

V

K

E

A

A

V

V

E

K

A

R

Q

Q

L

A

E

E

K

T

V

L

V

M

H

A

T

M

S

P

N

Q

I

T

F

L

L

P

N

T

I

P

P

M

V

R

L

G

E

E

L

F

A

Y

K

R

K

T

F

L

T

T

E

A

I

I

-

L

-

P

S

P

N

E

M

L

T

N

K

R

V

V

F

F

F

P

C

V

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

A

A

A

I

L

K

K

L

F

A

A

R

R

K

A

Y

H

S

T

F

-

D

-

K

-

H

-

G

-

K

-

A

G

R

R

N

K

T

K

I

F

L

V

T

A

L

A

K

M

K

R

S

G

F
|
F

H
x
S

G

G

R

R

T

T

I

M

T

G

T

S

L

L

K

S

A

V

G

T

G

W

Q

E

E

P

K

K

L

Y

H

R

K

E

Y

P

F

F

Y

L

P

P

F

L

T

V

E

E

G

P

F

V

K

E

Y

F

A

I

E

P

A

Y

N

N

-

D

V

V

E

E

E

A

L

L

E

K

K

R

S

A

I

V

D

D

S

E

I

T

C

A

A

A

I

V

M

I

I

L

E
|
E

P

P

I

V

Q

Q

G

G

E

E

G

G

I

V

N

R

P

P

L

A

S

T

E

P

E

E

F

F

V

L

H

R

K

A

V

A

F

R

G

E

I

I

A

T

E

Q

K

E

E

K

D

G

I

A

L

L

V

L

I

I

C

L

D
|
D

E

E

I
|
I

Q

Q

C

T

G

G

I

M

G

G

R

R

T

T

G

G

K

K

I

R

Y

F

G

A

F

F

N

E

N

H

Y

F

G

G

V

I

C

V

P

P

D

D

I

I

I

L

S

T

T

L

A

A

K
|
K

G

A

L

L

G

G

L

V

P

P

I

L

G

G

A

V

V

A

L

V

C

M

N

R

E

E

K

E

L

V

N

A

N

R

T

S

F

M

E

P

Y

K

G

G

D

G

H

H

G

G

S

T

T
|
T

F

F

G

G

N

N

P

P

V

L

C

A

A

M

A

A

G

A

A

G

L

V

E

A

V

A

L

I

N

R

I

Y

I

L

S

E

E

R

N

T

S

R

F

L

L

W

E

E

E

R

V

A

S

A

E

E

K

L

G

G

K

P

F

W

V

F

K

M

E

E

Y

K

F

L

K

R

S

A

K

I

N

P

K

S

K

P

N

K

I

I

L

R

E

E

V

V

R

R

G

G

M

M

G

G

L

L

M

M

I

V

G

G

I

L

E

E

I

L

-

K

E

E

G

K

E

A

A

A

S

P

R

Y

V

I

Q

A

K

R

K

L

A

E

L

K

Q

E

K

H

G

R

L

V

L

L

V

A

L

L

T

Q

A

A

G

G

P

P

N

T

V

V

V

I

R
|
R

L

F

L

L

P

P

L

L

I

V

I

I

S

E

K

K

E

E

E

D

L

L

E

E

active site: F132 (= F135), D217 (= D219), K246 (= K248), T275 (= T277), R363 (= R364)
binding pyridoxal-5'-phosphate: G105 (= G102), T106 (≠ A103), F132 (= F135), S133 (≠ H136), E184 (= E186), D217 (= D219), I219 (= I221), K246 (= K248)

Sites not aligning to the query:

active site: 13

4jewA N-acetylornithine aminotransferase from s. Typhimurium complexed with l-canaline
34% identity, 97% coverage: 9:387/390 of query aligns to 10:390/397 of 4jewA

query
sites
4jewA

E

E

Y

V

L

I

M

L

N

P

T

V

Y

Y

N

A

H

P

L

A

P

D

V

F

V

I

F

P

T

V

H
x
K

G

G

E
x
K

G

G

C

S

K

R

L

V

F
x
W

D

D

T

Q

D

Q

N

G

K

K

E

E

Y

Y

L

I

D

D

F

F

T

A

S

G

G

G

I

I

G

A

V

V

M

T

S

A

L

L

G

G

Y

H

G

C

N

H

K

P

N

A

W

L

I

V

K

E

A

A

V

L

E

K

A

S

Q

Q

L

G

E

E

K

T

V

L

V

W

H

H

T

T

S

S

N

N

I

V

F

F

L

T

N

N

I

E

P

P

V

A

L

L

E

R

L

L

A

G

K

R

K

K

F

L

T

I

E

D

I

A

S

T

N

F

M

A

T

E

K

R

V

V

F

L

F

F

C

M

N

N

S

S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

T

A

A

I

F

K

K

L

L

A

A

R

R

K

H

Y

Y

S

A

F

C

D

V

K

R

H

H

G

S

K

P

A

F

R

K

N

T

T

K

I

I

L

I

T

A

L

F

K

H

K

N

S

A

F
|
F

H
|
H

G

G

R
|
R

T

S

I

L

T

F

T

T

L

V

K

S

A

V

G

G

Q

Q

E

P

K

K

L

Y

H

S

K

D

Y

G

F

F

Y

G

P

P

F

K

T

P

E

A

G

D

F

I

K

I

Y

H

A

V

E

P

A

F

N

N

-

D

V

L

E

H

E

A

L

V

E

K

K

A

S

V

I

M

D

D

S

D

S

H

I

T

C

C

A

A

I

V

M

V

I

V

E
|
E

P

P

I

I

Q

Q

G

G

E
|
E

G

G

I

V

N

Q

P

A

L

A

S

T

E

P

E

E

F

F

V

L

H

K

K

G

V

L

F

R

G

D

I

L

A

C

E

D

K

E

E

H

D

Q

I

A

L

L

V

L

I

V

C

F

D
|
D

E

E

I
x
V

Q
|
Q

C

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

I

L

Y

F

G

A

F

Y

N

M

N

H

Y

Y

G

G

V

V

C

T

P

P

D

D

I

I

I

L

S

T

T

S

A

A

K
|
K

G

A

L

L

G

G

L

F

P

P

I

V

G

S

A

A

V

M

L

L

C

T

N

T

E

Q

K

E

L

I

N

A

N

S

T

A

F

F

E

-

Y

-

G

-

D

-

H

-

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

V

L

C

A

A

C

A

A

G

V

A

A

L

G

E

A

V

T

L

F

N

D

I

I

S

N

E

T

N

P

S

E

F

V

L

L

E

Q

E

G

V

I

-

H

S

T

E

K

K

R

G

Q

K

Q

F

F

V

V

K

Q

E

H

Y

L

F

Q

K

A

S

I

K

D

N

E

K

Q

K

F

N

D

I

I

L

F

-

S

E

D

V

I

R

R

G

G

M

M

G

G

L

L

M

L

I

I

G

G

I

A

E

E

I

L

-

K

-

P

-

K

-

Y

E

K

G

G

E

R

A

A

S

R

R

D

V

F

Q

L

K

Y

K

A

A

G

L

A

Q

E

K

A

G

G

L

V

L

M

V

V

L

L

T

N

A

A

G

G

P

A

N

D

V

V

V

M

R
|
R

L

F

L

A

P

P

P

S

L

L

I

V

S

E

K

E

E

A

E

D

L

I

E

H

A

E

G

G

L

M

N

Q

T

R

L

F

Y

A

E

Q

I

A

I

V

active site: F136 (= F135), E188 (= E186), D221 (= D219), Q224 (= Q222), K250 (= K248), T274 (= T277), R367 (= R364)
binding (2S)-2-azanyl-4-[(E)-[2-methyl-3-oxidanyl-5-(phosphonooxymethyl)pyridin-4-yl]methylideneamino]oxy-butanoic acid: G103 (= G102), T104 (≠ A103), F136 (= F135), H137 (= H136), R139 (= R138), E188 (= E186), E193 (= E191), D221 (= D219), V223 (≠ I221), K250 (= K248)
binding picric acid: K25 (≠ H24), K27 (≠ E26), W32 (≠ F31)

2pb0A Structure of biosynthetic n-acetylornithine aminotransferase from salmonella typhimurium: studies on substrate specificity and inhibitor binding (see paper)
34% identity, 97% coverage: 9:387/390 of query aligns to 4:384/389 of 2pb0A

query
sites
2pb0A

E

E

Y

V

L

I

M

L

N

P

T

V

Y

Y

N

A

H

P

L

A

P

D

V

F

V

I

F

P

T

V

H

K

G

G

E

K

G

G

C

S

K

R

L

V

F

W

D

D

T

Q

D

Q

N

G

K

K

E

E

Y

Y

L

I

D

D

F

F

T

A

S

G

G

G

I

I

G

A

V

V

M

T

S

A

L

L

G

G

Y

H

G

C

N

H

K

P

N

A

W

L

I

V

K

E

A

A

V

L

E

K

A

S

Q

Q

L

G

E

E

K

T

V

L

V

W

H

H

T

T

S

S

N

N

I

V

F

F

L

T

N

N

I

E

P

P

V

A

L

L

E

R

L

L

A

G

K

R

K

K

F

L

T

I

E

D

I

A

S

T

N

F

M

A

T

E

K

R

V

V

F

L

F

F

C

M

N

N

S
|
S

G
|
G

A
x
T

E

E

A

A

N

N

E

E

G

T

A

A

I

F

K

K

L

L

A

A

R

R

K

H

Y

Y

S

A

F

C

D

V

K

R

H

H

G

S

K

P

A

F

R

K

N

T

T

K

I

I

L

I

T

A

L

F

K

H

K

N

S

A

F
|
F

H
|
H

G

G

R

R

T

S

I

L

T

F

T

T

L

V

K

S

A

V

G

G

Q

Q

E

P

K

K

L

Y

H

S

K

D

Y

G

F

F

Y

G

P

P

F

K

T

P

E

A

G

D

F

I

K

I

Y

H

A

V

E

P

A

F

N

N

-

D

V

L

E

H

E

A

L

V

E

K

K

A

S

V

I

M

D

D

S

D

S

H

I

T

C

C

A

A

I

V

M

V

I

V

E
|
E

P

P

I

I

Q

Q

G

G

E

E

G

G

I

V

N

Q

P

A

L

A

S

T

E

P

E

E

F

F

V

L

H

K

K

G

V

L

F

R

G

D

I

L

A

C

E

D

K

E

E

H

D

Q

I

A

L

L

V

L

I

V

C

F

D
|
D

E

E

I
x
V

Q
|
Q

C

C

G

G

I

M

G

G

R

R

T

T

G

G

K

D

I

L

Y

F

G

A

F

Y

N

M

N

H

Y

Y

G

G

V

V

C

T

P

P

D

D

I

I

I

L

S

T

T

S

A

A

K
|
K

G

A

L

L

G

G

L

F

P

P

I

V

G

S

A

A

V

M

L

L

C

T

N

T

E

Q

K

E

L

I

N

A

N

S

T

A

F

F

E

-

Y

-

G

-

D

-

H

-

G

G

S

S

T
|
T

F

Y

G

G

N

N

P

P

V

L

C

A

A

C

A

A

G

V

A

A

L

G

E

A

V

A

L

F

N

D

I

I

S

N

E

T

N

P

S

E

F

V

L

L

E

Q

E

G

V

I

-

H

S

T

E

K

K

R

G

Q

K

Q

F

F

V

V

K

Q

E

H

Y

L

F

Q

K

A

S

I

K

D

N

E

K

Q

K

F

N

D

I

I

L

F

-

S

E

D

V

I

R

R

G

G

M

M

G

G

L

L

M

L

I

I

G

G

I

A

E

E

I

L

-

K

-

P

-

K

-

Y

E

K

G

G

E

R

A

A

S

R

R

D

V

F

Q

L

K

Y

K

A

A

G

L

A

Q

E

K

A

G

G

L

V

L

M

V

V

L

L

T

N

A

A

G

G

P

A

N

D

V

V

V

M

R
|
R

L

F

L

A

P

P

P

S

L

L

I

V

S

E

K

E

E

A

E

D

L

I

E

H

A

E

G

G

L

M

N

Q

T

R

L

F

Y

A

E

Q

I

A

I

V

active site: F130 (= F135), E182 (= E186), D215 (= D219), Q218 (= Q222), K244 (= K248), T268 (= T277), R361 (= R364)
binding pyridoxal-5'-phosphate: S96 (= S101), G97 (= G102), T98 (≠ A103), F130 (= F135), H131 (= H136), E182 (= E186), D215 (= D219), V217 (≠ I221), Q218 (= Q222), K244 (= K248)

A0QYS9 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium smegmatis) (see paper)
36% identity, 99% coverage: 1:388/390 of query aligns to 1:384/390 of A0QYS9

query
sites
A0QYS9

M

M

D

T

Y

L

L

Q

N

S

Y

R

A

W

K

E

E

A

Y

V

L

M

M

M

N

N

T

N

Y

Y

N

G

H

T

L

P

P

P

V

L

V

S

F

L

T

V

H

S

G

G

E

E

G

G

C

A

K

V

L

V

F

T

D

D

T

A

D

D

N

G

K

R

E

E

Y

Y

L

L

D

D

F

L

T

L

S

G

G

G

I

I

G

A

V

V

M

N

S

L

L

L

G

G

Y

H

G

R

N

H

K

P

N

A

W

V

I

I

K

E

A

A

V

V

E

T

A

T

Q

Q

L

L

E

D

K

T

V

L

V

G

H

H

T

T

S

S

N

N

I

L

F

Y

L

A

N

T

I

E

P

P

V

G

L

I

E

A

L

L

A

A

K

E

K

A

F

L

T

V

-

G

E

Q

I

L

S

G

N

T

M

Q

T

A

K

R

V

V

F

F

C

C

N

N

S

S

G

G

A

T

E

E

A

A

N

N

E

E

G

V

A

A

I

F

K

K

L

I

A

T

R

R

K

L

Y

-

S

-

F

-

D

-

K

-

H

-

G

-

K

T

A

G

R

K

N

T

T

K

I

I

L

V

T

A

L

A

K

E

K

G

S

A

F

F

H

H

G

G

R

R

T

T

I

M

T

G

T

S

L

L

K

A

A

L

G

T

G

G

Q

Q

E

P

K

S

L

K

H

Q

K

A

Y

P

F

F

Y

E

P

P

F

L

T

P

E

G

G

N

F

V

K

M

Y

H

A

V

E

P

-

Y

A

G

N

D

V

V

E

A

L

L

E

E

K

A

S

A

I

V

D

D

S

D

S

Q

I

T

C

A

A

A

I

V

M

F

I

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

G

I

V

N

V

P

V

L

P

S

P

E

A

E

G

F

Y

V

L

H

V

K

A

V

A

F

R

G

E

I

I

A

T

E

S

K

K

E

H

D

G

I

A

L

L

V

L

I

V

C

L

D

D

E

E

I

V

Q

Q

C

T

G

G

I

V

G

G

R

R

T

T

G

G

K

A

I

F

Y

F

G

A

F

H

N

Q

N

H

Y

D

G

G

V

I

C

V

P

P

D

D

I

V

S

T

T

M

A

A

K

K

G

G

L

L

G

G

L

L

P

P

I

I

G

G

A

A

V

C

L

L

C

A

N

V

E

G

K

A

L

T

N

G

N

D

T

L

F

L

E

T

Y

P

G

G

D

L

H

H

G

G

S

S

T

T

F

F

G

G

N

N

P

P

V

V

C

C

A

T

A

A

G

A

A

G

L

L

E

A

V

V

L

L

N

K

I

T

I

L

S

A

E

A

N

E

S

D

F

L

L

V

E

A

E

R

V

A

S

G

E

V

K

L

G

G

K
|
K

F

T

V

L

K

S

E

H

Y

G

F

I

K

E

S

E

K

L

N

G

K

H

K

P

N

L

I

V

L

D

E

K

V

V

R

R

G

G

M

K

G

G

L

L

M

L

I

Q

G

G

I

I

E

V

I

L

E

T

-

V

G

P

E

S

A

A

S

K

R

A

V

V

Q

E

K

T

K

A

A

A

L

R

Q

D

K

A

G

G

L

F

L

L

V

V

L

N

T

A

A

A

G

A

P

P

N

E

V

V

R

R

L

L

L

A

P

P

L

L

I

I

S

T

K

E

E

G

E

Q

L

I

E

E

A

A

G

F

L

I

N

T

T

A

L

L

Y

P

E

A

I

V

I

L

E

D

K304 (= K309) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

P9WPZ7 Acetylornithine aminotransferase; ACOAT; EC 2.6.1.11 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see 2 papers)
35% identity, 98% coverage: 8:389/390 of query aligns to 16:395/400 of P9WPZ7

query
sites
P9WPZ7

K

Q

E

A

Y

V

L

M

M

M

N

N

T

N

Y

Y

N

G

H

T

L

P

P

P

V

I

V

A

F

L

T

A

H

S

G

G

E

D

G

G

C

A

K

V

L

V

F

T

D

D

T

V

D

D

N

G

K

R

E

T

Y

Y

L

I

D

D

F

L

T

L

S

G

G

G

I

I

G

A

V

V

M

N

S

V

L

L

G

G

Y

H

G

R

N

H

K

P

N

A

W

V

I

I

K

E

A

A

V

V

E

T

A

R

Q

Q

L

M

E

S

K

T

V

L

V

G

H

H

T

T

S

S

N

N

I

L

F

Y

L

A

N

T

I

E

P

P

V

G

L

I

E

A

L

L

A

A

K

E

F

L

T

V

E

A

I

L

-

L

-

G

-

A

S

D

N

Q

M

R

T

T

K

R

V

V

F

F

C

C

N

N

S

S

G

G

A

A

E

E

A

A

N

N

E

E

G

A

A

A

I

F

K

K

L

L

A

S

R

R

K

L

Y

-

S

-

F

-

D

-

K

-

H

-

G

-

K

T

A

G

R

R

N

T

T

K

I

L

L

V

T

A

L

A

K

H

K

D

S

A

F

F

H

H

G

G

R

R

T

T

I

M

T

G

T

S

L

L

K

A

A

L

G

T

G

G

Q

Q

E

P

K

A

L

K

H

Q

K

T

Y

P

F

F

Y

A

P

P

F

L

T

P

E

G

G

D

F

V

K

T

Y

H

A

V

E

G

-

Y

A

G

N

D

V

V

E

D

E

A

L

L

E

A

K

A

S

A

I

V

D

D

S

D

S

H

I

T

C

A

A

A

I

V

M

F

I

L

E

E

P

P

I

I

Q

M

G

G

E

E

G

S

G

G

I

V

N

V

P

V

L

P

S

P

E

A

E

G

F

Y

V

L

H

A

K

A

V

A

F

R

G

D

I

I

A

T

E

A

K

R

E

R

D

G

I

A

L

L

V

L

I

V

C

L

D

D

E

E

I

V

Q

Q

C

T

G

G

I

M

G

G

R

R

T

T

G

G

K

A

I

F

Y

F

G

A

F

H

N

Q

N

H

Y

D

G

G

V

I

C

T

P

P

D

D

I

V

S

T

T

L

A

A

K

K

G

G

L

L

G

G

L

L

P

P

I

I

G

G

A

A

V

C

L

L

C

A

N

V

E

G

K

P

L

A

N

A

N

E

T

L

F

L

E

T

Y

P

G

G

D

L

H

H

G

G

S

S

T

T

F

F

G

G

N

N

P

P

V

V

C

C

A

A

G

A

A

A

L

L

E

A

V

V

L

L

N

R

I

V

I

L

S

A

E

S

N

D

S

G

F

L

L

V

E

R

V

A

S

E

E

V

K

L

G

G

K
|
K

F

S

V

L

K

R

E

H

Y

G

F

I

K

E

S

A

K

L

N

G

K

H

K

P

N

L

I

I

L

D

E

H

V

V

R

R

G

G

M

R

G

G

L

L

M

L

I

L

G

G

I

I

E

A

I

L

E

T

G

A

-

P

E

H

A

A

S

K

R

D

V

A

Q

E

K

A

K

T

A

A

L

R

Q

D

K

A

G

G

L

Y

L

L

V

V

L

N

T

A

A

A

G

A

P

P

N

D

V

V

V

I

R

R

L

L

L

A

P

P

L

L

I

I

S

A

K

E

E

A

E

Q

L

L

E

D

A

G

G

F

L

V

N

A

T

A

L

L

Y

P

E

A

I

I

I

L

E

D

E

R

K314 (= K309) modified: Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)

Sites not aligning to the query:

1 modified: Initiator methionine, Removed
2 modified: N-acetylthreonine; partial

Query Sequence

>WP_012101948.1 NCBI__GCF_000016505.1:WP_012101948.1
MDYLNYAKEYLMNTYNHLPVVFTHGEGCKLFDTDNKEYLDFTSGIGVMSLGYGNKNWIKA
VEAQLEKVVHTSNIFLNIPVLELAKKFTEISNMTKVFFCNSGAEANEGAIKLARKYSFDK
HGKARNTILTLKKSFHGRTITTLKAGGQEKLHKYFYPFTEGFKYAEANVEELEKSIDSSI
CAIMIEPIQGEGGINPLSEEFVHKVFGIAEKEDILVICDEIQCGIGRTGKIYGFNNYGVC
PDIISTAKGLGGGLPIGAVLCNEKLNNTFEYGDHGSTFGGNPVCAAGALEVLNIISENSF
LEEVSEKGKFVKEYFKSKNKKNILEVRGMGLMIGIEIEGEASRVQKKALQKGLLVLTAGP
NVVRLLPPLIISKEELEAGLNTLYEIIEEE

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory