SitesBLAST
Comparing WP_012102749.1 NCBI__GCF_000016505.1:WP_012102749.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6jixA The cyrstal structure of taurine:2-oxoglutarate aminotransferase from bifidobacterium kashiwanohense, in complex with plp and glutamate (see paper)
51% identity, 97% coverage: 11:448/452 of query aligns to 4:443/447 of 6jixA
- active site: W21 (= W28), Y141 (= Y148), D250 (= D254), K279 (= K283)
- binding glutamic acid: S20 (= S27), W21 (= W28), P82 (= P90), A83 (≠ G91), A83 (≠ G91), Y84 (= Y92), L310 (= L314)
- binding pyridoxal-5'-phosphate: G114 (= G121), A115 (= A122), Y141 (= Y148), H142 (= H149), E217 (= E221), D250 (= D254), V252 (= V256), M253 (= M257), K279 (= K283), L310 (= L314), T311 (= T315)
6fyqA The crystal structure of a new transaminase from the marine bacterium virgibacillus (see paper)
37% identity, 94% coverage: 16:439/452 of query aligns to 3:431/443 of 6fyqA
- active site: F15 (≠ W28), Y147 (= Y148), D243 (= D254), K272 (= K283)
- binding pyridoxal-5'-phosphate: G114 (= G121), S115 (≠ A122), Y147 (= Y148), H148 (= H149), G149 (= G150), E210 (= E221), D243 (= D254), V245 (= V256), I246 (≠ M257), K272 (= K283)
6io1B Crystal structure of a novel thermostable (s)-enantioselective omega- transaminase from thermomicrobium roseum (see paper)
37% identity, 94% coverage: 20:444/452 of query aligns to 12:448/448 of 6io1B
- active site: L20 (≠ W28), Y151 (= Y148), D257 (= D254), K286 (= K283)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G117 (= G120), G118 (= G121), A119 (= A122), N122 (= N125), Y151 (= Y148), H152 (= H149), D257 (= D254), V259 (= V256), I260 (≠ M257), K286 (= K283)
6s54A Transaminase from pseudomonas fluorescens (see paper)
34% identity, 95% coverage: 16:443/452 of query aligns to 8:448/453 of 6s54A
- active site: M20 (≠ W28), Y152 (= Y148), D258 (= D254), K287 (= K283)
- binding pyridoxal-5'-phosphate: G119 (= G121), S120 (≠ A122), Y152 (= Y148), H153 (= H149), G154 (= G150), E225 (= E221), D258 (= D254), V260 (= V256), I261 (≠ M257), K287 (= K283)
6g4dB Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp (see paper)
31% identity, 97% coverage: 14:450/452 of query aligns to 1:449/453 of 6g4dB
- active site: Y15 (≠ W28), Y146 (= Y148), D253 (= D254), K282 (= K283)
- binding pyridoxal-5'-phosphate: S112 (≠ G120), G113 (= G121), S114 (≠ A122), Y146 (= Y148), H147 (= H149), E220 (= E221), D253 (= D254), V255 (= V256), I256 (≠ M257), K282 (= K283)
6g4fA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with pmp (see paper)
31% identity, 97% coverage: 14:450/452 of query aligns to 1:449/451 of 6g4fA
- active site: Y15 (≠ W28), Y146 (= Y148), D253 (= D254), K282 (= K283)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S112 (≠ G120), G113 (= G121), S114 (≠ A122), Y146 (= Y148), H147 (= H149), E220 (= E221), D253 (= D254), V255 (= V256), I256 (≠ M257), K282 (= K283)
6g4eA Crystal structure of the omega transaminase from pseudomonas jessenii in complex with plp and 6-aminohexanoate (6-aca) (see paper)
31% identity, 97% coverage: 14:450/452 of query aligns to 1:449/451 of 6g4eA
- active site: Y15 (≠ W28), Y146 (= Y148), D253 (= D254), K282 (= K283)
- binding 6-aminohexanoic acid: K282 (= K283), R412 (≠ Y413), M414 (≠ H415)
- binding pyridoxal-5'-phosphate: S112 (≠ G120), G113 (= G121), S114 (≠ A122), Y146 (= Y148), H147 (= H149), E220 (= E221), D253 (= D254), V255 (= V256), I256 (≠ M257), K282 (= K283)
5ghgB Transaminase w58l with smba
30% identity, 97% coverage: 14:452/452 of query aligns to 2:433/433 of 5ghgB
- active site: Y16 (≠ W28), Y143 (= Y148), E217 (= E221), D250 (= D254), I253 (≠ M257), K279 (= K283), A401 (≠ M420)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S109 (≠ G120), G110 (= G121), S111 (≠ A122), Y143 (= Y148), H144 (= H149), E217 (= E221), D250 (= D254), V252 (= V256), I253 (≠ M257), K279 (= K283)
5lhaA Amine transaminase crystal structure from an uncultivated pseudomonas species in the pmp-bound form
31% identity, 92% coverage: 15:431/452 of query aligns to 2:431/447 of 5lhaA
- active site: Y146 (= Y148), D253 (= D254), K282 (= K283), T319 (= T315)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: G113 (= G121), S114 (≠ A122), Y146 (= Y148), H147 (= H149), G148 (= G150), E220 (= E221), D253 (= D254), K282 (= K283), Y318 (≠ L314), T319 (= T315)
5lh9D Amine transaminase crystal structure from an uncultivated pseudomonas species in the plp-bound (internal aldimine) form
31% identity, 92% coverage: 15:431/452 of query aligns to 4:433/449 of 5lh9D
- active site: Y148 (= Y148), D255 (= D254), K284 (= K283), T321 (= T315)
- binding pyridoxal-5'-phosphate: G115 (= G121), S116 (≠ A122), Y148 (= Y148), H149 (= H149), G150 (= G150), E222 (= E221), D255 (= D254), V257 (= V256), K284 (= K283)
3fcrA Crystal structure of putative aminotransferase (yp_614685.1) from silicibacter sp. Tm1040 at 1.80 a resolution
31% identity, 94% coverage: 11:437/452 of query aligns to 3:447/458 of 3fcrA
- active site: S20 (vs. gap), Y153 (= Y148), E227 (= E221), D260 (= D254), V263 (≠ M257), K289 (= K283), T326 (= T315), G430 (vs. gap)
- binding pyridoxal-5'-phosphate: G120 (= G121), S121 (≠ A122), Y153 (= Y148), H154 (= H149), E227 (= E221), D260 (= D254), V262 (= V256), K289 (= K283)
5kr6B Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
31% identity, 96% coverage: 12:446/452 of query aligns to 5:456/460 of 5kr6B
5g2qA The crystal structure of a s-selective transaminase from arthrobacter sp. With alanine bound (see paper)
33% identity, 96% coverage: 15:447/452 of query aligns to 6:456/461 of 5g2qA
- active site: Y142 (= Y148), D256 (= D254), K285 (= K283)
- binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: G115 (= G121), S116 (≠ A122), Y142 (= Y148), H143 (= H149), V229 (≠ S226), D256 (= D254), V258 (= V256), K285 (= K283), V315 (≠ G313), T317 (= T315)
3gjuA Crystal structure of a putative aminotransferase (mll7127) from mesorhizobium loti maff303099 at 1.55 a resolution
31% identity, 93% coverage: 12:433/452 of query aligns to 5:443/458 of 3gjuA
- active site: S21 (≠ W28), Y154 (= Y148), E228 (= E221), D261 (= D254), V264 (≠ M257), K290 (= K283), T327 (= T315), G430 (≠ M420)
- binding pyridoxal-5'-phosphate: S120 (≠ G120), G121 (= G121), S122 (≠ A122), Y154 (= Y148), H155 (= H149), E228 (= E221), D261 (= D254), V263 (= V256), K290 (= K283)
5g09D The crystal structure of a s-selective transaminase from bacillus megaterium bound with r-alpha-methylbenzylamine (see paper)
33% identity, 96% coverage: 15:447/452 of query aligns to 11:468/473 of 5g09D
- active site: Y147 (= Y148), D268 (= D254), K297 (= K283)
- binding [6-methyl-5-oxidanyl-4-[(~{E})-[(1~{R})-1-phenylethyl]iminomethyl]pyridin-3-yl]methyl dihydrogen phosphate: Y59 (≠ V63), G120 (= G121), S121 (≠ A122), Y147 (= Y148), H148 (= H149), E236 (= E221), A241 (≠ S226), D268 (= D254), V270 (= V256), K297 (= K283)
5g0aA The crystal structure of a s-selective transaminase from bacillus megaterium (see paper)
33% identity, 96% coverage: 15:447/452 of query aligns to 6:463/468 of 5g0aA
- active site: F19 (≠ W28), Y142 (= Y148), E231 (= E221), D263 (= D254), L266 (≠ M257), K292 (= K283), R436 (≠ M420)
- binding pyridoxal-5'-phosphate: G115 (= G121), S116 (≠ A122), Y142 (= Y148), H143 (= H149), G144 (= G150), E231 (= E221), D263 (= D254), V265 (= V256), L266 (≠ M257), K292 (= K283)
4e3qA Pmp-bound form of aminotransferase crystal structure from vibrio fluvialis (see paper)
31% identity, 96% coverage: 14:446/452 of query aligns to 1:447/452 of 4e3qA
- active site: F18 (≠ W28), Y149 (= Y148), E222 (= E221), D255 (= D254), I258 (≠ M257), K284 (= K283), T321 (= T315), V421 (≠ M420)
- binding 4'-deoxy-4'-aminopyridoxal-5'-phosphate: S115 (≠ G120), G116 (= G121), S117 (≠ A122), Y149 (= Y148), H150 (= H149), E222 (= E221), D255 (= D254), V257 (= V256), I258 (≠ M257), K284 (= K283), F320 (≠ L314), T321 (= T315)
5kr3A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
31% identity, 96% coverage: 14:446/452 of query aligns to 6:453/458 of 5kr3A
6gwiB The crystal structure of halomonas elongata amino-transferase (see paper)
29% identity, 96% coverage: 11:444/452 of query aligns to 1:443/450 of 6gwiB
- active site: F18 (≠ W28), Y149 (= Y148), D255 (= D254), K284 (= K283)
- binding pyridoxal-5'-phosphate: S115 (≠ G120), G116 (= G121), S117 (≠ A122), Y149 (= Y148), H150 (= H149), G151 (= G150), E222 (= E221), D255 (= D254), V257 (= V256), I258 (≠ M257), K284 (= K283)
5kr5A Directed evolution of transaminases by ancestral reconstruction. Using old proteins for new chemistries
31% identity, 96% coverage: 15:446/452 of query aligns to 4:452/455 of 5kr5A
- binding calcium ion: E66 (≠ K72), D70 (≠ N76), D412 (≠ K408), E447 (≠ K441)
- binding pyridoxal-5'-phosphate: S117 (≠ G120), G118 (= G121), S119 (≠ A122), Y151 (= Y148), H152 (= H149), G153 (= G150), E224 (= E221), D257 (= D254), V259 (= V256), K286 (= K283), W322 (≠ L314), T323 (= T315)
Query Sequence
>WP_012102749.1 NCBI__GCF_000016505.1:WP_012102749.1
MEILQNEELCLESNKIKEYDKKYNLHSWSAQKKLNPLVITKAEGIYFWDSTGKKYFDMSS
QLVNLNIGHGNKKVINAIKEQADKMPFIGPGYAVDVRSKLAAKVIEKAPENMGKVFFTLG
GADSNENAIKIAKMATGKFKIFSRYRSYHGASFGAANLTGEPRRYTCEPGIPGFVKFFDP
YIYRESIRFESEEEACEYYLGKLKEQLIYEGTETVAAIFLETVTGSNGVIIPPKGYLQGI
RKLCDEFGIVMVCDEVMAGWGRTGEWFACNNWDVEPDIITFAKGVTCGYVPMGGVIVSKK
IGEYFDDNVLMCGLTYNAHPLGCAAGCATIEVYEEENLIENSKKMGVVLGEKLEQIKQKH
ASVGDVRYIGLFSAVELVKDKNTREALVPYGRDPEGIMGKIVGMLKEKGFSTYSHENCIM
VAPPLIIKKEELEEAMDILDKVLDFVDEYIEK
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory