SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012103714.1 NCBI__GCF_000016505.1:WP_012103714.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

1vpeA Crystallographic analysis of phosphoglycerate kinase from the hyperthermophilic bacterium thermotoga maritima (see paper)
55% identity, 99% coverage: 4:398/398 of query aligns to 1:396/398 of 1vpeA

query
sites
1vpeA

N

E

K

K

K

M

T

T

I

I

E

R

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D

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V

D

D

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L

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K

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G

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K

R

R

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D

F

F

N

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V

V

P

P

L

V

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E

D

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I

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D

D

E

D

N

T

R
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L

I

I

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G

A

S

A

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L

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P

T

T

I

I

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K

Y

Y

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E

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G

A

A

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K

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E

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L

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P

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V

A

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R

L

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S

E

E

L

L

L

L

K

G

K

K

E

E

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K

F

F

A

V

A

P

D

-

D

-

N

A

V

V

V

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G

G

E

D

N

E

A

V

K

K

A

K

A

A

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V

K

E

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E

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L

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K

D

E

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G

D

E

V

V

I

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E

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N

T

T

R

R

Y

F

R

H

I

P

E

G

E

E

T

T

K

K

N

N

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D

D

P

N

E

F

L

S

A

K

K

E

F

L

W

A

A

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S

L

L

G

A

E

D

I

I

F

H

V

V

N

N

D

D

A

A

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F

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G

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T

A

A

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H

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R

A

A

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A

-

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G

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x
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K

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V

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S

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D

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x
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E

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G

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E

-

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D

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N

G

I

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W

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V

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-

A

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-

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G
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A

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S

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L

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F

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K

active site: R35 (= R38), K196 (= K202), G353 (= G355), G376 (= G378)
binding phosphoaminophosphonic acid-adenylate ester: G194 (= G200), A195 (≠ V201), K196 (= K202), K200 (= K206), G218 (= G224), A219 (≠ G225), N316 (= N321), P318 (= P323), G320 (= G325), V321 (= V326), E323 (= E328), G352 (= G354), G353 (= G355), D354 (= D356), S355 (= S357)

P36204 Bifunctional PGK/TIM; EC 2.7.2.3; EC 5.3.1.1 from Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) (see paper)
55% identity, 99% coverage: 3:398/398 of query aligns to 1:397/654 of P36204

query
sites
P36204

F

M

N

E

K

K

K

M

T

T

I

I

E

R

D

D

V

V

D

D

V

L

K

K

G

G

K

K

R

R

V

V

L

I

V

M

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

V

Q

K

E

D

G

G

K

V

I

V

T

Q

D

D

E

D

N

T

R
|
R

L

I

I

R

G

A

S

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

A

M

L

E

E

N

Q

N

G

A

A

K

K

V

V

I

I

L

L

C

L

S

S

H

H

L

L

G

G

K

R

P

P

K

K

G

G

E

E

V

P

K

S

P

P

E

E

M

F

S

S

L

L

L

A

P

P

V

V

A

A

K

K

R

R

L

L

S

S

E

E

L

L

L

L

K

G

K

K

E

E

V

V

V

K

F

F

A

V

A

P

D

-

D

-

N

A

V

V

V

V

G

G

E

D

N

E

A

V

K

K

A

K

A

A

V

V

K

E

N

E

M

L

K

K

D

E

G

G

D

E

V

V

I

L

L

L

L

L

Q

E

N

N

T

T

R
|
R

Y

F

R

H

I

P

E

G

E

E

T

T

K

K

N

N

Q

D

D

P

N

E

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A

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K

E

F

L

W

A

A

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S

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L

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A

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I

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H

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V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

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R

A

A

H

H

C

A

S

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N

V

V

G

G

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F

L

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P

P

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S

A

-

V

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C

A

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Y

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E

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K

E

E

L

I

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Y

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N

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S

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F

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V

A

V

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V

L

L

G

G

G

G

V

A

K

K

V

V

S

S

D

D

K

K

I

I

N

G

V

V

I

I

N

T

N

N

L

L

L

M

E

E

K

K

V

A

D

D

T

R

L

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M

Y

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D

K

K

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I

E

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Y

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A

A

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E

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K

A

A

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L

I

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L

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P

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D

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A

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A

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Q

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K

F

I

D

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S

G

A

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E

E

-

K

P

K

I

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V

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R

D

I

D

D

K

D

N

G

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I

K

P

D

E

G

G

Y

W

M

M

G

G

M

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D

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T

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W

N

N

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G

G

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V

F

F

E

E

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D

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E

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V

A

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A

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A

A

-

A

-

L

-

T

E

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G

G

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A

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I

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G

G

G

G

G

G

D

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S

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A

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N

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L

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H

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T

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G

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G

A

A

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S

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L

E

E

F

F

L

L

G

E

G

G

E

K

E

E

L

L

P

P

G

G

I

I

S

A

A

S

L

I

N

A

N

D

K

K

R36 (= R38) binding substrate
R118 (= R122) binding substrate
R151 (= R155) binding substrate

P40924 Phosphoglycerate kinase; EC 2.7.2.3 from Bacillus subtilis (strain 168) (see paper)
56% identity, 99% coverage: 3:398/398 of query aligns to 1:394/394 of P40924

query
sites
P40924

F

M

N

N

K

K

K

K

T

T

I

L

E

K

D

D

V

I

D

D

V

V

K

K

G

G

K

K

R

V

V

V

L

F

V

C

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

Q

K

E

D

G

G

K

E

I

V

T

T

D

D

E

D

N

T

R

R

L

I

I

R

G

A

S

A

L

L

P

P

T

T

I

I

K

K

Y

H

L

L

M

A

E

D

N

Q

N

G

A

A

K

K

V

V

I

L

L

L

C

A

S

S

H

H

L

L

G

G

K

R

P

P

K

K

G

G

E

E

V

V

K

V

P

E

E

E

M

L

S

R

L

L

L

T

P

P

V

V

A

A

K

A

R

R

L

L

S

G

E

E

L

L

L

L

K

G

K

K

E

E

V

V

V

K

F

K

A

A

A

-

D

-

D

D

N

E

V

A

V

Y

G

G

E

D

N

A

A

V

K

K

A

A

A

Q

V

I

K

S

N

E

M

M

K

K

D

D

G

G

D

D

V

V

I

L

L

V

L

L

Q

E

N

N

T

V

R

R

Y

F

R

Y

I

P

E

G

E

E

T

E

K

K

N

N

Q

D

D

P

N

E

F

L

S

A

K

K

E

A

L

F

A

A

S

E

L

L

G

A

E

D

I

V

F

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

C

A

S

S

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T

V

A

G

G

V

I

T

A

K

E

F

H

L

L

P

P

T

-

A

A

V

V

C

A

G

G

Y

F

L

L

I

M

Q

E

K

K

E

E

L

L

E

D

F

V

L

L

G

G

N

K

A

A

I

V

E
x
S

N

N

P

P

S

D

R

R

P

P

F

F

T

T

A

A

I

I

L

I

G

G

G

G

V

A

K

K

V

V

S

K

D

D

K

K

I

I

N

G

V

V

I

I

N

E

N

S

L

L

L

L

E

D

K

K

V

V

D

D

T

N

L

L

I

I

I

I

G

G

G

G

G

G

M

L

S

A

Y

Y

T

T

F

F

A

V

R

K

A

A

Q

L

G

G

Y

Y

T

E

I

V

G

G

T

K

S

S

V

L

V

L

E

E

E

E

D

D

K

K

I

I

E

E

Y

L

A

A

K

K

E

S

M

F

I

M

D

D

K

R

A

A

K

K

E

E

K

K

G

G

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K

N

L

F

L

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L

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P

P

I

E

D

D

R

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L

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T

A

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D

K

D

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F

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D

Y

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E

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A

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K

T
|
T

A

R

K

E

V

T

Y

Y

A

A

D

D

A

V

I

I

K

K

D

N

S

S

K

K

T

L

I

V

I

V

W

W

N

N

G

G

P

P

M

M

G

G

V

V

F

F

E

E

F

I

K

D

N

L

F

F

A

A

K

Q

G

G

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T

F

K

A

A

V

V

A

A

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E

A

A

M

L

A

A

E

E

S

A

-

K

G

D

A

T

I

Y

T

S

I

V

I

I

G

G

G

G

G

G

D

D

S

S

A

A

A

A

A

A

I

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N

E

Q

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L

F

G

G

F

L

G

A

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D

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K

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M

T

S

H

H

I

I

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S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

F

L

M

G

E

G

G

E

K

E

E

L

L

P

P

G

G

I

V

S

A

A

A

L

L

N

N

N

D

K

K

S183 (≠ E188) modified: Phosphoserine
T299 (= T304) modified: Phosphothreonine

1phpA Structure of the adp complex of the 3-phosphoglycerate kinase from bacillus stearothermophilus at 1.65 angstroms (see paper)
55% identity, 99% coverage: 3:398/398 of query aligns to 1:394/394 of 1phpA

query
sites
1phpA

F

M

N

N

K

K

K

K

T

T

I

I

E

R

D

D

V

V

D

D

V

V

K

R

G

G

K

K

R

R

V

V

L

F

V

C

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

Q

E

E

Q

G

G

K

A

I

I

T

T

D

D

E

D

N

T

R
|
R

L

I

I

R

G

A

S

A

L

L

P

P

T

T

I

I

K

R

Y

Y

L

L

M

I

E

E

N

H

N

G

A

A

K

K

V

V

I

I

L

L

C

A

S

S

H

H

L

L

G

G

K

R

P

P

K

K

G

G

E

K

V

V

K

V

P

E

E

E

M

L

S

R

L

L

L

D

P

A

V

V

A

A

K

K

R

R

L

L

S

G

E

E

L

L

L

L

K

E

K

R

E

P

V

V

V

-

F

-

A

A

A

K

D

T

D

N

N

E

V

A

V

V

G

G

E

D

N

E

A

V

K

K

A

A

A

A

V

V

K

D

N

R

M

L

K

N

D

E

G

G

D

D

V

V

I

L

L

L

L

L

Q

E

N

N

T

V

R

R

Y

F

R

Y

I

P

E

G

E

E

T

E

K

K

N

N

Q

D

D

P

N

E

F

L

S

A

K

K

E

A

L

F

A

A

S

E

L

L

G

A

E

D

I

L

F

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

T

V

E

G

G

V

I

T

A

K

H

F

Y

L

L

P

P

T

-

A

A

V

V

C

A

G

G

Y

F

L

L

I

M

Q

E

K

K

E

E

L

L

E

E

F

V

L

L

G

G

N

K

A

A

I

L

E

S

N

N

P

P

S

D

R

R

P

P

F

F

T

T

A

A

I

I

L

I

G

G

G
|
G

V

A

K
|
K

V

V

S

K

D

D

K
|
K

I

I

N

G

V

V

I

I

N

D

N

N

L

L

L

L

E

E

K

K

V

V

D

D

T

N

L

L

I

I

I

I

G

G

G
|
G

G
|
G

M

L

S

A

Y

Y

T

T

F

F

A

V

R

K

A

A

Q

L

G

G

Y

H

T

D

I

V

G

G

T

K

S

S

V
x
L

V

L

E

E

E

E

D

D

K

K

I

I

E

E

Y

L

A

A

K

K

E

S

M

F

I

M

D

E

K

K

A

A

K

K

E

E

K

K

G

G

I

V

K

R

L

F

L

Y

L

M

P

P

I

V

D

D

R

V

V

V

V

V

T

A

D

D

K

R

F

F

D

A

E

N

S

D

A

A

E

N

P

T

I

K

L

V

E

V

D

P

D

I

K

D

N

A

I

I

K

P

D

A

G

D

Y

W

M

S

G

A

M

L

D

D

I

I

G

G

P

P

K

K

T

T

A

R

K

E

V

L

Y

Y

A

R

D

D

A

V

I

I

K

R

D

E

S

S

K

K

T

L

I

V

I

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

K

D

N

A

F

F

A

A

K

H

G

G

T

T

-

K

F

A

A

I

V

A

A

E

K

A

A

L

M

A

A

E

E

A

S

L

G

D

A

T

I

Y

T

S

I

V

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

A

A

A

I

V

N

E

Q

K

L

F

G

G

F

L

G

A

D

D

K

K

M

M

T

D

H

H

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

F

L

M

G

E

G

G

E

K

E

Q

L

L

P

P

G

G

I

V

S

V

A

A

L

L

N

E

N

D

K

K

active site: R36 (= R38), K197 (= K202), G351 (= G355), G374 (= G378)
binding adenosine-5'-diphosphate: G195 (= G200), K201 (= K206), G219 (= G224), G220 (= G225), L237 (≠ V242), N316 (= N321), P318 (= P323), G320 (= G325), V321 (= V326), E323 (= E328), G350 (= G354), D352 (= D356), S353 (= S357)

P18912 Phosphoglycerate kinase; EC 2.7.2.3 from Geobacillus stearothermophilus (Bacillus stearothermophilus) (see paper)
55% identity, 99% coverage: 3:398/398 of query aligns to 1:394/394 of P18912

query
sites
P18912

F

M

N

N

K

K

K

K

T

T

I

I

E

R

D

D

V

V

D

D

V

V

K

R

G

G

K

K

R

R

V

V

L

F

V

C

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

M

Q

E

E

Q

G

G

K

A

I

I

T

T

D

D

E

D

N

T

R

R

L

I

I

R

G

A

S

A

L

L

P

P

T

T

I

I

K

R

Y

Y

L

L

M

I

E

E

N

H

N

G

A

A

K

K

V

V

I

I

L

L

C

A

S

S

H

H

L

L

G

G

K

R

P

P

K

K

G

G

E

K

V

V

K

V

P

E

E

E

M

L

S

R

L

L

L

D

P

A

V

V

A

A

K

K

R

R

L

L

S

G

E

E

L

L

L

L

K

E

K

R

E

P

V

V

V

-

F

-

A

A

A

K

D

T

D

N

N

E

V

A

V

V

G

G

E

D

N

E

A

V

K

K

A

A

A

A

V

V

K

D

N

R

M

L

K

N

D

E

G

G

D

D

V

V

I

L

L

L

L

L

Q

E

N

N

T

V

R

R

Y

F

R

Y

I

P

E

G

E

E

T

E

K

K

N

N

Q

D

D

P

N

E

F

L

S

A

K

K

E

A

L

F

A

A

S

E

L

L

G

A

E

D

I

L

F

Y

V

V

N

N

D

D

A

A

F

F

G

G

T

A

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

T

V

E

G

G

V

I

T

A

K

H

F

Y

L

L

P

P

T

-

A

A

V

V

C

A

G

G

Y

F

L

L

I

M

Q

E

K

K

E

E

L

L

E

E

F

V

L

L

G

G

N

K

A

A

I

L

E

S

N

N

P

P

S

D

R

R

P

P

F

F

T

T

A

A

I

I

L

I

G

G

G

G

V

A

K

K

V

V

S

K

D

D

K
|
K

I

I

N

G

V

V

I

I

N

D

N

N

L

L

L

L

E

E

K

K

V

V

D

D

T

N

L

L

I

I

I

I

G

G

G

G

G

G

M

L

S

A

Y

Y

T

T

F

F

A

V

R

K

A

A

Q

L

G

G

Y

H

T

D

I

V

G

G

T

K

S

S

V

L

V

L

E

E

E

E

D

D

K

K

I

I

E

E

Y

L

A

A

K

K

E

S

M

F

I

M

D

E

K

K

A

A

K

K

E

E

K

K

G

G

I

V

K

R

L

F

L

Y

L

M

P

P

I

V

D

D

R

V

V

V

V

V

T

A

D

D

K

R

F

F

D

A

E

N

S

D

A

A

E

N

P

T

I

K

L

V

E

V

D

P

D

I

K

D

N

A

I

I

K

P

D

A

G

D

Y

W

M

S

G

A

M

L

D

D

I

I

G

G

P

P

K

K

T

T

A

R

K

E

V

L

Y

Y

A

R

D

D

A

V

I

I

K

R

D

E

S

S

K

K

T

L

I

V

I

V

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

F

M

K

D

N

A

F

F

A

A

K

H

G

G

T

T

-

K

F

A

A

I

V

A

A

E

K

A

A

L

M

A

A

E

E

A

S

L

G

D

A

T

I

Y

T

S

I

V

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

A

A

A

I

V

N

E

Q

K

L

F

G

G

F

L

G

A

D

D

K

K

M

M

T

D

H

H

I

I

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

F

L

M

G

E

G

G

E

K

E

Q

L

L

P

P

G

G

I

V

S

V

A

A

L

L

N

E

N

D

K

K

K201 (= K206) binding ATP
N316 (= N321) binding ATP
E323 (= E328) binding ATP
GGDS 350:353 (= GGDS 354:357) binding ATP

16pkA Phosphoglycerate kinase from trypanosoma brucei bisubstrate analog (see paper)
46% identity, 99% coverage: 5:398/398 of query aligns to 2:415/415 of 16pkA

query
sites
16pkA

K

K

K

K

T

S

I

I

E

N

D

E

V

C

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

V

Q

K

E

N

G

G

K

K

I

I

T

T

D

N

E

D

N

Y

R
|
R

L

I

I

R

G

S

S

A

L

L

P

P

T

T

I

L

K

K

Y

K

L

V

M

L

E

T

N

E

N

G

A

G

K

S

V

C

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

P

P

K

K

G

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

E

G

V

F

K

Q

P

Q

E

K

M

A

S

T

L

L

L

K

P

P

V

V

A

A

K

K

R

R

L

L

S

S

E

E

L

L

L

L

K

L

K

R

E

P

V

V

V

T

F

F

A

A

A

P

D

D

D

-

N

-

V

-

V

-

G

C

E

L

N

N

A

A

K

A

A

D

A

V

V

V

K

S

N

K

M

M

K

S

D

P

G

G

D

D

V

V

I

V

L

L

L

L

Q

E

N

N

T

V

R

R

Y

F

R

Y

I

K

E

E

E

E

-

G

-

S

-

K

-

K

T

A

K

K

N

D

Q

R

D

E

N

A

F

M

S

A

K

K

E

I

L

L

A

A

S

S

L

Y

G

G

E

D

I

V

F

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

C

A

S

T

T

M

V

T

G

G

V

I

T

P

K

K

F

I

L

L

P

G

T

N

A

G

V

A

C

A

G

G

Y

Y

L

L

I

M

Q

E

K

K

E

E

L

I

E

S

F

Y

L

F

G

A

N

K

A

V

I

L

E

G

N

N

P

P

S

P

R

R

P

P

F

L

T

V

A

A

I

I

L

V

G

G

G
|
G

V
x
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

N

Q

V

L

I

L

N

D

N

N

L

M

L

L

E

Q

K

R

V

I

D

D

T

Y

L

L

I

L

I

I

G

G

G

G

G
x
A

M

M

S

A

Y
|
Y

T

T

F

F

A

L

R

K

A

A

Q

Q

G

G

Y

Y

T

S

I

I

G

G

T

K

S

S

V

K

V

C

E

E

E

E

D

S

K

K

I

L

E

E

Y

F

A

A

K

R

E

S

M

L

I

L

D

K

K

K

A

A

K

E

E

D

K

R

G

K

I

V

K

Q

L

V

L

I

L

L

P

P

I

I

D

D

R

H

V

V

V

C

T

H

D

T

K

E

F

F

D

K

E

A

S

V

A

D

E

S

P

P

I

L

L

I

E

T

D

E

D

D

K

Q

N

N

I

I

K

P

D

E

G

G

Y

H

M

M

G

A

M
x
L

D

D

I

I

G

G

P

P

K

K

T

T

A

I

K

E

V

K

Y

Y

A

V

D

Q

A

T

I

I

K

G

D

K

S

C

K

K

T

S

I

A

I

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

K

V

N

P

F

Y

A

S

K

K

G

G

T

T

F

F

A

A

V

I

A

A

K

K

A

A

M

M

A

G

-

R

-

G

-

T

-

H

E

E

S

H

G

G

A

L

I

M

T

S

I

I

I

I

G

G

G

G

G
|
G

D

D

S

S

A

A

A

S

A

A

I

A

N

E

Q

L

L

S

G

G

F

E

G

A

D

K

K

R

M

M

T

S

H

H

I

V

S

S

T

T

G
|
G

G
|
G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

G

E

G

G

E

K

E

T

L

L

P

P

G

G

I

V

S

T

A

V

L

L

N

D

N

D

K

K

active site: R35 (= R38), K215 (= K202), G372 (= G355), G395 (= G378)
binding 1,1,5,5-tetrafluorophosphopentylphosphonic acid adenylate ester: G213 (= G200), A214 (≠ V201), K219 (= K206), A238 (≠ G225), Y241 (= Y228), L311 (≠ M298), P336 (= P323), G338 (= G325), V339 (= V326), E341 (= E328), G393 (= G376), G394 (= G377), G395 (= G378)

13pkA Ternary complex of phosphoglycerate kinase from trypanosoma brucei (see paper)
46% identity, 99% coverage: 5:398/398 of query aligns to 2:415/415 of 13pkA

query
sites
13pkA

K

K

K

K

T

S

I

I

E

N

D

E

V

C

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

V

Q

K

E

N

G

G

K

K

I

I

T

T

D

N

E

D

N

Y

R
|
R

L

I

I

R

G

S

S

A

L

L

P

P

T

T

I

L

K

K

Y

K

L

V

M

L

E

T

N

E

N

G

A

G

K

S

V

C

I

V

L

L

C

M

S

S

H

H

L

L

G

G

K

R

P

P

K

K

G

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

E

G

V

F

K

Q

P

Q

E

K

M

A

S

T

L

L

L

K

P

P

V

V

A

A

K

K

R

R

L

L

S

S

E

E

L

L

L

L

K

L

K

R

E

P

V

V

V

T

F

F

A

A

A

P

D

D

D

-

N

-

V

-

V

-

G

C

E

L

N

N

A

A

K

A

A

D

A

V

V

V

K

S

N

K

M

M

K

S

D

P

G

G

D

D

V

V

I

V

L

L

L

L

Q

E

N

N

T

V

R

R

Y

F

R

Y

I

K

E

E

E

E

-

G

-

S

-

K

-

K

T

A

K

K

N

D

Q

R

D

E

N

A

F

M

S

A

K

K

E

I

L

L

A

A

S

S

L

Y

G

G

E

D

I

V

F

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

D

H

S

C

A

S

T

T

M

V

T

G

G

V

I

T

P

K

K

F

I

L

L

P

G

T

N

A

G

V

A

C

A

G

G

Y

Y

L

L

I

M

Q

E

K

K

E

E

L

I

E

S

F

Y

L

F

G

A

N

K

A

V

I

L

E

G

N

N

P

P

S

P

R

R

P

P

F

L

T

V

A

A

I

I

L

V

G

G

G
|
G

V
x
A

K
|
K

V

V

S

S

D

D

K
|
K

I

I

N

Q

V

L

I

L

N

D

N

N

L

M

L

L

E

Q

K

R

V

I

D

D

T

Y

L

L

I

L

I

I

G

G

G

G

G

A

M

M

S

A

Y

Y

T

T

F

F

A

L

R

K

A

A

Q

Q

G

G

Y

Y

T

S

I

I

G

G

T

K

S

S

V

K

V

C

E

E

E

E

D

S

K

K

I

L

E

E

Y

F

A

A

K

R

E

S

M

L

I

L

D

K

K

K

A

A

K

E

E

D

K

R

G

K

I

V

K

Q

L

V

L

I

L

L

P

P

I

I

D

D

R

H

V

V

V

C

T

H

D

T

K

E

F

F

D

K

E

A

S

V

A

D

E

S

P

P

I

L

L

I

E

T

D

E

D

D

K

Q

N

N

I

I

K

P

D

E

G

G

Y

H

M

M

G

A

M
x
L

D

D

I

I

G

G

P

P

K

K

T

T

A

I

K

E

V

K

Y

Y

A

V

D

Q

A

T

I

I

K

G

D

K

S

C

K

K

T

S

I

A

I

I

W

W

N

N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

M

K

V

N

P

F

Y

A

S

K

K

G

G

T

T

F

F

A

A

V

I

A

A

K

K

A

A

M

M

A

G

-

R

-

G

-

T

-

H

E

E

S

H

G

G

A

L

I

M

T

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

S

A

A

I

A

N

E

Q

L

L

S

G

G

F

E

G

A

D

K

K

R

M

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

G

E

G

G

E

K

E

T

L

L

P

P

G

G

I

V

S

T

A

V

L

L

N

D

N

D

K

K

active site: R35 (= R38), K215 (= K202), G372 (= G355), G395 (= G378)
binding adenosine-5'-diphosphate: G213 (= G200), A214 (≠ V201), K219 (= K206), L311 (≠ M298), P336 (= P323), G338 (= G325), V339 (= V326), E341 (= E328), G371 (= G354), D373 (= D356), S374 (= S357)

P07378 Phosphoglycerate kinase, glycosomal; Phosphoglycerate kinase C; EC 2.7.2.3 from Trypanosoma brucei brucei (see 2 papers)
45% identity, 100% coverage: 1:398/398 of query aligns to 1:419/440 of P07378

query
sites
P07378

M

M

V

T

F

L

N

N

-

E

K

K

K

K

T

S

I

I

E

N

D

E

V

C

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

I

R

R

C

V

D
|
D

F
|
F

N
|
N

V

V

P

P

L

V

Q

K

E

N

G

G

K

K

I

I

T

T

D

N

E

D

N

Y

R
|
R

L

I

I

R

G

S

S

A

L

L

P

P

T

T

I

L

K

K

Y

K

L

V

M

L

E

T

N

E

N

G

A

G

K

S

V

C

I

V

L

L

C

M

S

S

H
|
H

L
|
L

G
|
G

K
x
R

P

P

K

K

G

G

-

I

-

P

-

M

-

A

-

Q

-

A

-

G

-

K

-

I

-

R

-

S

-

T

-

G

-

G

-

V

-

P

E

G

V

F

K

Q

P

Q

E

K

M

A

S

T

L

L

L

K

P

P

V

V

A

A

K

K

R

A

L

L

S

S

E

E

L

L

L

L

K

L

K

R

E

P

V

V

V

T

F

F

A

A

A

P

D

D

D

-

N

-

V

-

V

-

G

C

E

L

N

N

A

A

K

A

A

D

A

V

V

V

K

S

N

K

M

M

K

S

D

P

G

G

D

D

V

V

I

V

L

L

L

L

Q

E

N

N

T

V

R
|
R

Y

F

R

Y

I

K

E

E

E

E

-

G

-

S

-

K

-

K

T

A

K

K

N

D

Q

R

D

E

N

A

F

M

S

A

K

K

E

I

L

L

A

A

S

S

L

Y

G

G

E

D

I

V

F

Y

V

I

N

S

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

D

H

S

C

A

S

T

T

M

V

T

G

G

V

I

T

P

K

K

F

I

L

L

P

G

T

N

A

G

V

A

C

A

G

G

Y

Y

L

L

I

M

Q

E

K

K

E

E

L

I

E

S

F

Y

L

F

G

A

N

K

A

V

I

L

E

G

N

N

P

P

S

P

R

R

P

P

F

L

T

V

A

A

I

I

L

V

G

G

G

G

V

A

K

K

V

V

S

S

D

D

K
|
K

I

I

N

Q

V

L

I

L

N

D

N

N

L

M

L

L

E

Q

K

R

V

I

D

D

T

Y

L

L

I

L

I

I

G

G

G

G

G

A

M

M

S

A

Y

Y

T

T

F

F

A

L

R

K

A

A

Q

Q

G

G

Y

Y

T

S

I

I

G

G

T

K

S

S

V

K

V

C

E

E

E

E

D

S

K

K

I

L

E

E

Y

F

A

A

K

R

E

S

M

L

I

L

D

K

K

K

A

A

K

E

E

D

K

R

G

K

I

V

K

Q

L

V

L

I

L

L

P

P

I

I

D

D

R

H

V

V

V

C

T

H

D

T

K

E

F

F

D

K

E

A

S

V

A

D

E

S

P

P

I

L

L

I

E

T

D

E

D

D

K

Q

N

N

I

I

K

P

D

E

G

G

Y

H

M

M

G

A

M

L

D

D

I

I

G

G

P

P

K

K

T

T

A

I

K

E

V

K

Y

Y

A

V

D

Q

A

T

I

I

K

G

D

K

S

C

K

K

T

S

I

A

I

I

W

W

N
|
N

G

G

P

P

M

M

G

G

V

V

F

F

E
|
E

F

M

K

V

N

P

F

Y

A

S

K

K

G

G

T

T

F

F

A

A

V

I

A

A

K

K

A

A

M

M

A

G

-

R

-

G

-

T

-

H

E

E

S

H

G

G

A

L

I

M

T

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

S

A

A

I

A

N

E

Q

L

L

S

G

G

F

E

G

A

D

K

K

R

M

M

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

L

L

L

G

E

G

G

E

K

E

T

L

L

P

P

G

G

I

V

S

T

A

V

L

L

N

D

N

E

K

K

DFN 24:26 (= DFN 23:25) binding substrate
R39 (= R38) binding substrate
HLGR 62:65 (≠ HLGK 61:64) binding substrate
R135 (= R122) binding substrate
R172 (= R155) binding substrate
K223 (= K206) binding ATP
N338 (= N321) binding ATP
E345 (= E328) binding ATP
GGDS 375:378 (= GGDS 354:357) binding ATP

P00560 Phosphoglycerate kinase; EC 2.7.2.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
47% identity, 99% coverage: 4:398/398 of query aligns to 5:415/416 of P00560

query
sites
P00560

N

S

K

K

K

L

T

S

I

V

E

Q

D

D

V

L

D

D

V

L

K

K

G

D

K

K

R

R

V

V

L

F

V

I

R
|
R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

Q

D

E

G

G

K

K

K

I

I

T

T

D

S

E

N

N

Q

R
|
R

L

I

I

V

G

A

S

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

V

M

L

E

E

N

H

N

H

A

P

K

R

-

Y

V

V

I

V

L

L

C

A

S

S

H

H

L

L

G

G

K

R

P

P

K

N

G

G

E

E

V

R

K

N

P

E

E

K

M

Y

S

S

L

L

L

A

P

P

V

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

L

L

K

G

K

K

E

D

V

V

V

T

F

F

A

L

A

N

D

D

D

-

N

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

A

A

A

A

V

V

K

K

N

A

M

S

K

A

D

P

G

G

D

S

V

V

I

I

L

L

L

L

Q

E

N

N

T

L

R
|
R

Y

Y

R

H

I

I

E

E

E

E

T

E

K

G

N

S

Q

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

-

K

-

E

-

D

-

V

D

Q

N

K

F

F

S

R

K

H

E

E

L

L

A

S

S

S

L

L

G

A

E

D

I

V

F

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R
|
R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

F

T

D

K

-

F

-

L

L

P

P

T

Q

A

R

V

A

C

A

G

G

Y

F

L

L

I

L

Q

E

K

K

E

E

L

L

E

K

F

Y

L

F

G

G

N

K

A

A

I

L

E

E

N

N

P

P

S

T

R

R

P

P

F

F

T

L

A

A

I

I

L

L

G

G

G

G

V

A

K

K

V

V

S

A

D

D

K

K

I

I

N

Q

V

L

I

I

N

D

N

N

L

L

L

L

E

D

K

K

V

V

D

D

T

S

L

I

I

I

I

I

G

G

G

G

G

G

M

M

S

A

Y

F

T

T

F

F

A

K

R

K

A

V

-

L

Q

E

G

N

Y

T

T

E

I

I

G

G

T

D

S

S

V

I

V

F

E

D

E

K

D

A

K

G

I

A

E

E

Y

I

A

V

K

P

E

K

M

L

I

M

D

E

K

K

A

A

K

K

E

A

K

K

G

G

I

V

K

E

L

V

L

V

L

L

P

P

I

V

D

D

R

F

V

I

V

I

T

A

D

D

K

A

F

F

D

S

E

A

S

D

A

A

E

N

P

T

-

K

I

T

L

V

E

T

D

D

D

K

K

E

N

G

I

I

K

P

D

A

G

G

Y

W

M

Q

G

G

M

L

D

D

I

N

G

G

P

P

K

E

T

S

A

R

K

K

V

L

Y

F

A

A

D

A

A

T

I

V

K

A

D

K

S

A

K

K

T

T

I

I

I

V

W

W

N

N

G

G

P

P

M

P

G

G

V

V

F

F

E

E

F

F

K

E

N

K

F

F

A

A

K

A

G

G

T

T

F

K

A

A

V

L

A

L

K

D

A

E

M

V

A

V

E

K

S

S

G

S

A

A

-

A

-

G

-

N

I

T

T

V

I

I

I

I

G

G

G

G

G

G

D

D

S

T

A

A

A

T

A

V

I

A

N

K

Q

K

L

Y

G

G

F

V

G

T

D

D

K

K

M

I

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G

G

A

A

S

S

L

L

E

E

F

L

L

L

G

E

G

G

E

K

E

E

L

L

P

P

G

G

I

V

S

A

A

F

L

L

N

S

N

E

K

K

R22 (= R21) mutation to K: 2-fold reduction of Vmax.; mutation to M: 7-fold reduction of Vmax.
R39 (= R38) binding substrate
R122 (= R122) binding substrate
R169 (= R155) binding substrate

1qpgA 3-phosphoglycerate kinase, mutation r65q (see paper)
47% identity, 99% coverage: 4:398/398 of query aligns to 4:414/415 of 1qpgA

query
sites
1qpgA

N

S

K

K

K

L

T

S

I

V

E

Q

D

D

V

L

D

D

V

L

K

K

G

D

K

K

R

R

V

V

L

F

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

Q

D

E

G

G

K

K

K

I

I

T

T

D

S

E

N

N

Q

R
|
R

L

I

I

V

G

A

S

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

V

M

L

E

E

N

H

N

H

A

P

K

R

-

Y

V

V

I

V

L

L

C

A

S

S

H

H

L

L

G

G

K

Q

P

P

K

N

G

G

E

E

V

R

K

N

P

E

E

K

M

Y

S

S

L

L

L

A

P

P

V

V

A

A

K

K

R

E

L

L

S

Q

E

S

L

L

L

L

K

G

K

K

E

D

V

V

V

T

F

F

A

L

A

N

D

D

D

-

N

-

V

C

V

V

G

G

E

P

N

E

A

V

K

E

A

A

A

A

V

V

K

K

N

A

M

S

K

A

D

P

G

G

D

S

V

V

I

I

L

L

L

L

Q

E

N

N

T

L

R

R

Y

Y

R

H

I

I

E

E

E

E

T

E

K

G

N

S

Q

R

-

K

-

V

-

D

-

G

-

Q

-

K

-

V

-

K

-

A

-

S

-

K

-

E

-

D

-

V

D

Q

N

K

F

F

S

R

K

H

E

E

L

L

A

S

S

S

L

L

G

A

E

D

I

V

F

Y

V

I

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

S

S

S

T

M

V

V

G

G

V

F

T

D

K

-

F

-

L

L

P

P

T

Q

A

R

V

A

C

A

G

G

Y

F

L

L

I

L

Q

E

K

K

E

E

L

L

E

K

F

Y

L

F

G

G

N

K

A

A

I

L

E

E

N

N

P

P

S

T

R

R

P

P

F

F

T

L

A

A

I

I

L

L

G

G

G

G

V

A

K
|
K

V

V

S

A

D

D

K

K

I

I

N

Q

V

L

I

I

N

D

N

N

L

L

L

L

E

D

K

K

V

V

D

D

T

S

L

I

I

I

I

I

G

G

G
|
G

G
|
G

M

M

S

A

Y

F

T

T

F

F

A

K

R

K

A

V

-

L

Q

E

G

N

Y

T

T

E

I

I

G

G

T

D

S

S

V

I

V

F

E

D

E

K

D

A

K

G

I

A

E

E

Y

I

A

V

K

P

E

K

M

L

I

M

D

E

K

K

A

A

K

K

E

A

K

K

G

G

I

V

K

E

L

V

L

V

L

L

P

P

I

V

D

D

R

F

V

I

V

I

T

A

D

D

K

A

F

F

D

S

E

A

S

D

A

A

E

N

P

T

-

K

I

T

L

V

E

T

D

D

D

K

K

E

N

G

I

I

K

P

D

A

G

G

Y

W

M

Q

G

G

M

L

D

D

I

N

G

G

P

P

K

E

T

S

A

R

K

K

V

L

Y

F

A

A

D

A

A

T

I

V

K

A

D

K

S

A

K

K

T

T

I

I

I

V

W

W

N
|
N

G

G

P
|
P

M

P

G
|
G

V
|
V

F
|
F

E
|
E

F

F

K

E

N

K

F

F

A

A

K

A

G

G

T

T

F

K

A

A

V

L

A

L

K

D

A

E

M

V

A

V

E

K

S

S

G

S

A

A

-

A

-

G

-

N

I

T

T

V

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
x
T

A

A

A

T

A

V

I

A

N

K

Q

K

L

Y

G

G

F

V

G

T

D

D

K

K

M

I

T

S

H

H

I

V

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

L

E

E

F

L

L

L

G

E

G

G

E

K

E

E

L

L

P

P

G

G

I

V

S

A

A

F

L

L

N

S

N

E

K

K

active site: R38 (= R38), K213 (= K202), G371 (= G355), G394 (= G378)
binding magnesium-5'-adenyly-imido-triphosphate: G235 (= G224), G236 (= G225), N334 (= N321), P336 (= P323), G338 (= G325), V339 (= V326), F340 (= F327), E341 (= E328), G370 (= G354), G371 (= G355), D372 (= D356), T373 (≠ S357)

3zlbA Crystal structure of phosphoglycerate kinase from streptococcus pneumoniae (see paper)
50% identity, 99% coverage: 5:398/398 of query aligns to 3:398/398 of 3zlbA

query
sites
3zlbA

K

K

K

L

T

T

I

V

E

K

D
|
D

V

V

D

D

V

L

K

K

G

G

K

K

R

K

V

V

L

L

V

V

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

Q

K

E

D

G

G

K

V

I

I

T

T

D

N

E

D

N

N

R
|
R

L

I

I

T

G

A

S

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

I

M

I

E

E

N

Q

N

G

A

G

K

R

V

A

I

I

L

L

C

F

S

S

H

H

L

L

G

G

K

R

P

V

K

K

G

E

E

E

V

S

-

D

K

K

P

A

E

G

M

K

S

S

L

L

L

A

P

P

V

V

A

A

K

A

R

D

L

L

S

A

E

A

L

K

L

L

K

G

K

Q

E

D

V

V

V

V

F

F

A

P

A

G

D

V

D

T

N

R

V

-

V

-

G

G

E

A

N

E

A

L

K

E

A

A

A

A

V

I

K

N

N

A

M

L

K

E

D

D

G

G

D

Q

V

V

I

L

L

L

L

V

Q

E

N

N

T

T

R

R

Y

Y

R

E

-

D

-

V

-

D

-

G

I

K

E

K

E

E

T

S

K

K

N

N

Q

D

D

P

N

E

F

L

S

G

K

K

E

Y

L

W

A

A

S

S

L

L

G

G

E

D

-

G

I

I

F

F

V

V

N

N

D

D

A

A

F

F

G

G

T

T

A

A

H

H

R

R

A

A

H

H

C

A

S

S

T

N

V

V

G

G

V

I

T

S

K

A

F

N

L

V

P

E

T

K

A

A

V

V

C

A

G

G

Y

F

L

L

I

L

Q

E

K

N

E

E

L

I

E

A

F

Y

L

I

G

Q

N

E

A

A

I

V

E

E

N

T

P

P

S

E

R

R

P

P

F

F

T

V

A

A

I

I

L

L

G

G

G
|
G

V
x
S

K
|
K

V

V

S

S

D

D

K

K

I

I

N

G

V

V

I

I

N

E

N

N

L

L

L

L

E

E

K

K

V

A

D

D

T

K

L

V

I

L

I

I

G

G

G
|
G

G
|
G

M

M

S

T

Y

Y

T

T

F

F

A

Y

R

K

A

A

Q

Q

G

G

Y

I

T

E

I

I

G

G

T

N

S

S

V

L

V

V

E

E

E

E

D

D

K

K

I

L

E

D

Y

V

A

A

K

K

E

A

M

L

I

L

D

E

K

K

A

A

K

N

E

G

K

-

G

-

I

-

K

K

L

L

L

I

L

L

P

P

I

V

D

D

R

S

V

K

V

E

T

A

D

N

K

A

F

F

D

A

E

G

S

Y

A

T

E

E

P

V

I

R

L

D

E

T

D

E

D

G

K

E

N

A

I

V

K

S

D

E

G

G

Y

F

M

L

G

G

M

L

D

D

I

I

G

G

P

P

K

K

T

S

A

I

K

A

V

K

Y

F

A

D

D

E

A

A

I

L

K

T

D

G

S

A

K

K

T

T

I

V

I

V

W

W

N
|
N

G

G

P
|
P

M

M

G
|
G

V
|
V

F

F

E
|
E

F

N

K

P

N

D

F

F

A

Q

K

A

G

G

T

T

F

I

A

G

V

V

A

M

K

D

A

A

M

I

A

V

-

K

E

Q

S

P

G

G

A

V

I

K

T

S

I

I

I

I

G

G

G
|
G

G
|
G

D
|
D

S
|
S

A

A

A

A

A

A

I

A

N

I

Q

N

L

L

G

G

F

R

G

A

D

D

K

K

M

F

T

S

H

W

I

I

S

S

T

T

G

G

G

G

G
|
G

A

A

S

S

L

M

E

E

F

L

L

L

G

E

G

G

E

K

E

V

L

L

P

P

G

G

I

L

S

A

A

A

L

L

N

T

N

E

K
|
K

active site: R36 (= R38), K204 (= K202), G355 (= G355), G378 (= G378)
binding phosphoaminophosphonic acid-adenylate ester: G202 (= G200), S203 (≠ V201), G226 (= G224), G227 (= G225), N320 (= N321), P322 (= P323), G324 (= G325), V325 (= V326), E327 (= E328), G354 (= G354), G355 (= G355), D356 (= D356), S357 (= S357)
binding magnesium ion: D8 (= D10), K398 (= K398)

Sites not aligning to the query:

binding magnesium ion: 1

O60101 Phosphoglycerate kinase; EC 2.7.2.3 from Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) (see paper)
50% identity, 99% coverage: 4:398/398 of query aligns to 5:414/414 of O60101

query
sites
O60101

N

T

K

K

K

L

T

A

I

I

E

T

D

D

V

V

D

D

V

L

K

K

G

G

K

K

R

N

V

V

L

L

V

I

R

R

C

V

D

D

F

F

N

N

V

V

P

P

L

L

Q

D

E

G

G

D

K

R

I

I

T

T

D

N

E

N

N

A

R

R

L

I

I

V

G

G

S

A

L

L

P

P

T

T

I

I

K

K

Y

Y

L

A

M

L

E

E

N

Q

N

Q

A

P

K

K

-

A

V

V

I

I

L

L

C

M

S

S

H

H

L

L

G

G

K

R

P

P

K

N

G

G

E

A

V