SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012103744.1 NCBI__GCF_000016505.1:WP_012103744.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

P9WP55 O-acetylserine sulfhydrylase; OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase; EC 2.5.1.47 from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
53% identity, 99% coverage: 2:302/303 of query aligns to 3:305/310 of P9WP55

query
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P9WP55

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K44 (= K43) modified: N6-(pyridoxal phosphate)lysine
N74 (= N73) binding pyridoxal 5'-phosphate
GTGGT 178:182 (≠ GTAGT 176:180) binding pyridoxal 5'-phosphate
S266 (= S264) binding pyridoxal 5'-phosphate

2q3dA 2.2 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) from mycobacterium tuberculosis in complex with the reaction intermediate alpha-aminoacrylate (see paper)
53% identity, 99% coverage: 2:302/303 of query aligns to 3:305/306 of 2q3dA

query
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2q3dA

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active site: K44 (= K43), S266 (= S264), P293 (= P290)
binding 2-[(3-hydroxy-2-methyl-5-phosphonooxymethyl-pyridin-4-ylmethyl)-amino]-propionic acid: K44 (= K43), T71 (= T70), S72 (= S71), N74 (= N73), T75 (= T74), Q144 (= Q143), V177 (= V175), G178 (= G176), T179 (= T177), G180 (≠ A178), T182 (= T180), G222 (= G220), I223 (= I221), S266 (= S264), P293 (= P290), D294 (= D291)

3zeiA Structure of the mycobacterium tuberculosis o-acetylserine sulfhydrylase (oass) cysk1 in complex with a small molecule inhibitor (see paper)
53% identity, 98% coverage: 2:297/303 of query aligns to 3:300/300 of 3zeiA

query
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active site: K44 (= K43), S266 (= S264), P293 (= P290)
binding 3-[(Z)-[(5Z)-5-[[2-(2-hydroxy-2-oxoethyloxy)phenyl]methylidene]-3-methyl-4-oxidanylidene-1,3-thiazolidin-2-ylidene]amino]benzoic acid: T71 (= T70), S72 (= S71), I126 (= I125), Q144 (= Q143), F145 (= F144), K215 (≠ T213), G222 (= G220), A225 (= A223), F227 (= F225)
binding pyridoxal-5'-phosphate: K44 (= K43), N74 (= N73), V177 (= V175), G178 (= G176), T179 (= T177), G180 (≠ A178), T182 (= T180), G222 (= G220), S266 (= S264), P293 (= P290), D294 (= D291)

2q3cA 2.1 a resolution crystal structure of o-acetylserine sulfhydrylase (oass) holoenzyme from mycobacterium tuberculosis in complex with the inhibitory peptide dfsi (see paper)
53% identity, 98% coverage: 2:297/303 of query aligns to 3:300/300 of 2q3cA

query
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2q3cA

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L

active site: K44 (= K43), S266 (= S264), P293 (= P290)
binding : T71 (= T70), S72 (= S71), G73 (= G72), T75 (= T74), M122 (= M121), Q144 (= Q143), K215 (≠ T213), G222 (= G220), A225 (= A223)

4lmbA Crystal structure analysis of o-acetylserine sulfhydrylase cysk2 complexed with cystine from microcystis aeruginosa 7806 (see paper)
52% identity, 100% coverage: 1:302/303 of query aligns to 2:308/310 of 4lmbA

query
sites
4lmbA

M

L

I

I

F

A

N

R

N

D

A

I

I

T

D

Q

M

L

I

V

G

G

A

R

T

T

P

P

L

L

F

V

K

Q

L

L

D

N

N

R

F

I

K

P

N

V

K

A

D

E

-

G

-

V

S

K

A

A

E

R

I

I

Y

V

V

V

K

K

L

L

E

E

K

S

Y

M

N

N

P

P

G

A

S

S

I

V

K
|
K

D

D

R

R

A

I

A

G

L

V

G

S

M

M

I

V

E

E

K

D

A

A

E

E

K

A

D

A

G

G

L

L

I

I

E

H

K

P

G

D

G

K

T

T

I

I

-

L

V

V

E

E

P

P

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

M

I

V

G

A

K

A

L

A

K

K

G

G

Y

Y

K

R

V

L

I

V

I

L

V

T

M

M

P

P

E

E

T

T

M
|
M

S

S

V

L

E

E

R

R

N

A

M

M

I

L

K

K

A

A

Y

Y

G

G

A

A

E

Q

L

L

V

E

L

L

T

T

D

P

G

G

T

S

K

Q

G

G

M
|
M

K

E

G

G

A

A

I

I

E

T

K

R

A

A

Y

E

E

E

I

I

A

V

K

E

N

N

K

T

R

P

G

N

Y

A

Y

Y

I

S

P

L

Q

Q

Q
|
Q

F
|
F

I

R

N

N

K

P

A

A

N

N

P

P

K

K

K

I

H

H

Y

R

E

E

T

T

A

A

E

E

I

I

L

W

E

A

D

D

L

T

Q

D

-

G

H

L

V

V

D

D

A

I

F

V

V

I

A

G

G

G

V
|
V

G
|
G

T
|
T

A
x
G

G

G

T
|
T

L

I

A

T

G

G

V

I

G

A

E

E

N

T

L

I

K

K

G

P

R

R

D

R

K

P

N

Q

V

F

K

Q

I

A

I

I

A

A

V

V

E

E

P

P

A

S

S

N

S

S

P

P

V

V

L

L

S

S

G

G

Q

Q

T

P

G

G

A

P

H

H

K

K

I

I

Q
|
Q

G
|
G

I
|
I

G

G

A
|
A

G

G

F

F

V

I

P

P

D

A

I

I

Y

F

I

R

P

P

E

E

L

L

V

I

D

D

K

E

I

V

I

I

T

I

I

V

T

D

D

D

E

T

T

E

A

A

F

F

K

A

Y

Y

A

A

R

R

L

R

M

L

G

A

K

R

E

Q

E

E

G

G

I

L

L

L

V

S

G

G

I

I

S
|
S

S

A

G

G

A

A

N

A

I

L

A

W

A

A

I

I

Q

Q

V

V

A

G

E

K

E

R

L

P

-

E

G

N

K

E

G

D

K

K

K

L

V

I

V

V

T

M

V

I

A

Q

P
|
P

D

S

G

F

G

G

E

E

K

R

Y

Y

L

L

S

S

M

T

G

A

L

L

Y

F

active site: K46 (= K43), S269 (= S264)
binding cysteine: K46 (= K43), T74 (= T70), S75 (= S71), N77 (= N73), T78 (= T74), M101 (= M97), M125 (= M121), M125 (= M121), Q147 (= Q143), F148 (= F144), Q224 (= Q219), G225 (= G220), G225 (= G220), I226 (= I221), A228 (= A223)
binding pyridoxal-5'-phosphate: K46 (= K43), N77 (= N73), V180 (= V175), G181 (= G176), T182 (= T177), G183 (≠ A178), T185 (= T180), G225 (= G220), S269 (= S264), P296 (= P290)

4lmaA Crystal structure analysis of o-acetylserine sulfhydrylase cysk1 from microcystis aeruginosa 7806 (see paper)
51% identity, 100% coverage: 2:303/303 of query aligns to 3:309/318 of 4lmaA

query
sites
4lmaA

I

I

F

A

N

H

N

D

A

V

I

T

D

E

M

L

I

V

G

G

A

R

T

T

P

P

L

L

F

V

K

Q

L

L

D

N

N

R

F

I

K

P

N

Q

K

A

D

E

S

G

A

C

-

L

-

G

E

R

I

I

Y

V

V

M

K

K

L

L

E

E

K

G

Y

M

N

N

P

P

G

A

S

S

I

V

K
|
K

D

D

R

R

A

I

A

G

L

T

G

H

M

M

I

I

E

N

K

S

A

A

E

E

K

K

D

A

G

G

L

L

I

I

E

N

K

P

G

E

G

T

T

T

I

V

-

L

V

V

E

E

P

P

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

M

I

T

G

A

K

A

L

A

K

K

G

G

Y

Y

K

R

V

L

I

I

I

L

V

T

M

M

P

P

E

E

T

T

M

M

S

S

V

L

E

E

R

R

N

A

M

M

I

L

K

K

A

A

Y

Y

G

G

A

A

E

T

L

L

V

E

L

L

T

T

D

P

G

G

T

S

K

Q

G

G

M

M

K

K

G

G

A

A

I

I

E

L

K

R

A

A

Y

Q

E

Q

I

I

A

V

K

D

N

S

K

I

R

P

G

G

Y

A

Y

Y

I

M

P

L

Q

Q

F

F

I

R

N

N

K

P

A

S

N

N

P

P

K

E

K

I

H

H

Y

R

E

L

T

T

A

A

E

E

I

I

L

W

E

Q

D

D

L

T

Q

E

-

G

H

Q

V

V

D

D

A

F

F

I

V

V

A

A

G

G

V

V

G
|
G

T
|
T

A
x
G

G

G

T
|
T

L

I

A

T

G

G

V

V

G

A

E

E

N

V

L

I

K

K

G

S

R

R

D

K

K

P

N

S

V

F

K

Q

I

V

A

A

V

V

E

E

P

P

A

F

S

N

S

S

P

P

V

V

L

I

S

S

G

G

Q

N

T

P

G

G

A

P

H

H

K

K

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

I

P

P

D

E

I

V

Y

L

I

R

P

T

E

D

L

L

V

I

D

D

K

E

I

V

I

I

T

T

I

V

T

S

D

D

E

E

T

E

A

A

F

F

K

Q

Y

F

A

G

R

R

L

R

M

L

G

A

K

K

E

E

G

G

I

L

L

L

V

S

G

G

I

I

S
|
S

S

S

G

G

A

A

N

N

I

L

A

C

A

A

I

I

Q

Q

V

L

A

A

E

Q

E

R

L

P

-

E

G

N

K

E

G

G

K

K

K

L

V

I

V

V

T

V

V

I

A

Q

P
|
P

D

S

G

F

G

G

E

E

K

R

Y

Y

L

L

S

S

M

T

G

L

L

M

Y

F

D

Q

active site: K46 (= K43), S269 (= S264)
binding pyridoxal-5'-phosphate: K46 (= K43), N77 (= N73), G181 (= G176), T182 (= T177), G183 (≠ A178), T185 (= T180), G225 (= G220), S269 (= S264), P296 (= P290)

5xemA Crystal structure of a hydrogen sulfide-producing enzyme (fn1220) from fusobacterium nucleatum in complex with l-lanthionine-plp schiff base
53% identity, 97% coverage: 4:298/303 of query aligns to 4:301/302 of 5xemA

query
sites
5xemA

N

N

N

S

A

V

I

I

D

D

M

L

I

I

G

G

A

N

T

T

P

P

L

L

F

V

K

K

L

I

D

N

N

N

F

I

K

-

N

D

K

T

D

F

S

G

A

N

E

E

I

I

Y

Y

V

V

K

K

L

L

E

E

K

G

Y

S

N

N

P

P

G

G

G

R

S

S

I

T

K
|
K

D

D

R

R

A

I

A

A

L

L

G

K

M

M

I

I

E

E

K

E

A

A

E

E

K

K

D

E

G

G

L

L

I

I

E

D

K

K

G

D

G

T

T

V

I

I

V

I

E

E

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

G

C

K

A

L

V

K

K

G

N

Y

Y

K

K

V

L

I

K

I

I

V

V

M

M

P

P

E

D

T

T

M

M

S

S

V

I

E

E

R

R

R

I

N

Q

M

L

I

M

K

R

A

A

Y

Y

G

G

A

T

E

E

L

V

V

I

L

L

T

T

D

D

G

G

T

S

K

L

G

G

M
|
M

K

K

G

A

A

C

I

L

E

E

K

K

A

L

Y

E

E

E

I

L

A

K

K

K

N

N

K

E

R

K

G

K

Y

Y

Y

F

I

V

P

P

Q

N

Q
|
Q

F

F

I

T

N

N

K

V

A

N

N

N

P

P

K

K

K

A

H

H

Y

Y

E

E

T

T

A

A

E

E

I

I

L

L

E

K

D

D

L

L

Q

N

H

N

-

K

V

V

D

D

A

V

F

F

V

I

A

C

G

G

V
x
T

G
|
G

T
|
T

A
x
G

G

G

T
x
S

L

F

A

S

G

G

V

T

G

A

E

K

N

K

L

L

K

K

G

E

R

K

D

L

K

P

N

N

V

I

K

K

I

T

I

F

A

P

V

V

E

E

P

P

A

A

S

S

P

P

V

L

L

L

S

S

G

K

G

G

Q

Y

T

I

G

G

A

P

H

H

K

K

I

I

Q

Q

G
|
G

I
x
M

G
|
G

A

M

-
x
S

-

I

G

G

F

G

V

I

P

P

D

A

I

V

Y

Y

I

D

P

G

E

S

L

L

V

A

D

D

K

D

I

I

I

L

T

V

I

C

T

E

D

D

E

D

T

D

A

A

F

F

K

E

Y

M

A

M

R

R

L

E

M

L

G

S

K

F

E

K

E

E

G

G

I

I

L

L

V

G

G

G

I

I

S
|
S

S

T

G

G

A

A

N

T

I

F

A

K

A

A

I

L

Q

D

V

Y

A

S

E

K

E

E

-

N

L

A

G

D

K

K

G

G

K

L

K

K

V

I

V

V

T

V

V

L

A

S

P
x
T

D
|
D

G

S

G

G

E

E

K

K

Y

Y

L
|
L

S
|
S

binding (2R)-2-azanyl-3-[(2R)-2-azanyl-3-oxidanyl-3-oxidanylidene-propyl]sulfanyl-propanoic acid: K42 (= K43), T69 (= T70), S70 (= S71), N72 (= N73), T73 (= T74), M120 (= M121), Q142 (= Q143), G176 (= G176), T177 (= T177), G220 (= G220), M221 (≠ I221), G222 (= G222), S224 (vs. gap)
binding calcium ion: L300 (= L297), S301 (= S298)
binding pyridoxal-5'-phosphate: K42 (= K43), N72 (= N73), T175 (≠ V175), G176 (= G176), T177 (= T177), G178 (≠ A178), S180 (≠ T180), G220 (= G220), S266 (= S264), T293 (≠ P290), D294 (= D291)

Sites not aligning to the query:

binding calcium ion: 302

5xenA Crystal structure of a hydrogen sulfide-producing enzyme (fn1220) from fusobacterium nucleatum in complex with l-serine-plp schiff base
53% identity, 97% coverage: 4:297/303 of query aligns to 4:300/300 of 5xenA

query
sites
5xenA

N

N

N

S

A

V

I

I

D

D

M

L

I

I

G

G

A

N

T

T

P

P

L

L

F

V

K

K

L

I

D

N

N

N

F

I

K

-

N

D

K

T

D

F

S

G

A

N

E

E

I

I

Y

Y

V

V

K

K

L

L

E

E

K

G

Y

S

N

N

P

P

G

G

G

R

S

S

I

T

K
|
K

D

D

R

R

A

I

A

A

L

L

G

K

M

M

I

I

E

E

K

E

A

A

E

E

K

K

D

E

G

G

L

L

I

I

E

D

K

K

G

D

G

T

T

V

I

I

V

I

E

E

P

A

T
|
T

S
|
S

G

G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

G

C

K

A

L

V

K

K

G

N

Y

Y

K

K

V

L

I

K

I

I

V

V

M

M

P

P

E

D

T

T

M

M

S

S

V

I

E

E

R

R

R

I

N

Q

M

L

I

M

K

R

A

A

Y

Y

G

G

A

T

E

E

L

V

V

I

L

L

T

T

D

D

G

G

T

S

K

L

G

G

M

M

K

K

G

A

A

C

I

L

E

E

K

K

A

L

Y

E

E

E

I

L

A

K

K

K

N

N

K

E

R

K

G

K

Y

Y

Y

F

I

V

P

P

Q

N

Q
|
Q

F

F

I

T

N

N

K

V

A

N

N

N

P

P

K

K

K

A

H

H

Y

Y

E

E

T

T

A

A

E

E

I

I

L

L

E

K

D

D

L

L

Q

N

H

N

-

K

V

V

D

D

A

V

F

F

V

I

A

C

G

G

V
x
T

G
|
G

T
|
T

A
x
G

G

G

T
x
S

L

F

A

S

G

G

V

T

G

A

E

K

N

K

L

L

K

K

G

E

R

K

D

L

K

P

N

N

V

I

K

K

I

T

I

F

A

P

V

V

E

E

P

P

A

A

S

S

P

P

V

L

L

L

S

S

G

K

G

G

Q

Y

T

I

G

G

A

P

H

H

K

K

I

I

Q

Q

G
|
G

I

M

G

G

A

M

-

S

-

I

G

G

F

G

V

I

P

P

D

A

I

V

Y

Y

I

D

P

G

E

S

L

L

V

A

D

D

K

D

I

I

I

L

T

V

I

C

T

E

D

D

E

D

T

D

A

A

F

F

K

E

Y

M

A

M

R

R

L

E

M

L

G

S

K

F

E

K

E

E

G

G

I

I

L

L

V

G

G

G

I

I

S
|
S

S

T

G

G

A

A

N

T

I

F

A

K

A

A

I

L

Q

D

V

Y

A

S

E

K

E

E

-

N

L

A

G

D

K

K

G

G

K

L

K

K

V

I

V

V

T

V

V

L

A

S

P
x
T

D
|
D

G

S

G

G

E

E

K

K

Y

Y

L

L

binding pyridoxal-5'-phosphate: K42 (= K43), K42 (= K43), N72 (= N73), N72 (= N73), T175 (≠ V175), T175 (≠ V175), G176 (= G176), G176 (= G176), T177 (= T177), T177 (= T177), G178 (≠ A178), G178 (≠ A178), S180 (≠ T180), S180 (≠ T180), G220 (= G220), G220 (= G220), S266 (= S264), S266 (= S264), T293 (≠ P290), T293 (≠ P290), D294 (= D291), D294 (= D291)
binding serine: K42 (= K43), T69 (= T70), S70 (= S71), N72 (= N73), T73 (= T74), Q142 (= Q143)

5xoqA Crystal structure of o-acetylserine sulfhydrylase with bound transcription factor peptide inhibitor from planctomyces limnophilus
51% identity, 100% coverage: 2:303/303 of query aligns to 4:307/310 of 5xoqA

query
sites
5xoqA

I

I

F

F

N

K

N

D

A

N

I

S

D

E

M

S

I

I

G

G

A

R

T

T

P

P

L

L

F

V

K

Q

L

I

D

N

N

R

F

L

K

T

N

A

K

G

D

L

S

S

A

S

E

R

I

V

Y

L

V

A

K

K

L

I

E

E

K

G

Y

R

N

N

P

P

G

A

G

Y

S

S

I

V

K

K

D

C

R

R

A

I

A

G

L

A

G

A

M

M

I

I

E

W

K

D

A

A

E

E

K

Q

D

S

G

G

L

K

I

L

E

K

K

P

G

G

G

M

T

H

I

V

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N

N

T
|
T

G

G

I

I

A

A

L

L

A

A

M

F

I

V

G

C

K

A

L

A

K

R

G

G

Y

Y

K

K

V

L

I

T

I

L

V

T

M

M

P

P

E

E

T

T

M

M

S

S

V

I

E

E

R

R

N

M

M

M

I

L

K

K

A

S

Y

F

G

G

A

A

E

D

L

L

V

V

L

L

T

T

D

P

G

G

T

A

K

D

G

G

M
|
M

K

K

G

G

A

A

I

I

E

S

K

K

A

A

Y

E

E

E

I

L

A

A

K

A

N

Q

K

P

R

-

G

G

Y

W

Y

F

I

I

P

P

Q

Q

Q
|
Q

F

F

I

K

N

N

K

P

A

A

N

N

P

P

K

A

K

I

H

H

Y

V

E

K

T

T

A

G

E

P

E

E

I

I

L

W

E

N

D

D

L

T

Q

E

-

G

H

Q

V

V

D

D

A

V

F

F

V

V

A

A

G

G

V

V

G

G

T

T

A

G

G

G

T

T

L

I

A

T

G

G

V

V

G

A

E

R

N

F

L

L

K

K

G

H

R

E

D

K

K

K

N

H

-

P

V

V

K

H

I

V

A

A

V

V

E

E

P

P

A

A

S

A

S

S

P

P

V

V

L

L

S

A

G

G

Q

P

T

A

G

G

A
x
R

H
|
H

K

K

I

I

Q
|
Q

G
|
G

I

I

G

G

A
|
A

G

G

F

F

V

V

P

P

D

D

I

T

Y

F

I

D

P

R

E

S

L

V

V

V

D

D

K

E

I

I

I

L

T

S

I

V

T

T

D

D

E

D

T

E

A

A

F

I

K

E

Y

T

A

A

R

R

L

K

M

L

G

A

K

M

E

E

G

G

I

I

L

S

V

C

G

G

I

I

S

S

S

C

G

G

A

A

N

A

I

M

A

A

A

G

A

A

I

L

Q

K

V

V

A

A

E

A

E

R

L

P

G

E

-

F

K

A

G

G

K

K

K

T

V

I

V

V

T

T

V

V

A

L

P

P

D

D

G

A

G

G

E

E

K

R

Y

Y

L

L

S

S

M

T

G

A

L

L

Y

F

D

E

binding : T72 (= T70), S73 (= S71), G74 (= G72), T76 (= T74), M123 (= M121), Q144 (= Q143), R218 (≠ A215), H219 (= H216), Q222 (= Q219), G223 (= G220), A226 (= A223)

2efyA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-acetylbutyric acid
52% identity, 97% coverage: 10:302/303 of query aligns to 7:301/302 of 2efyA

query
sites
2efyA

I

I

G

G

A

K

T

T

P

P

L

V

F

V

K

R

L

L

D

A

N

K

F

V

K

V

N

E

K

P

D

D

S

M

A

A

E

E

I

V

Y

W

V

V

K

K

L

L

E

E

K

G

Y

L

N

N

P

P

G

G

S

S

I

I

K
|
K

D

D

R

R

A

P

A

A

L

W

G

Y

M

M

I

I

E

K

K

D

A

A

E

E

K

E

D

R

G

G

L

I

I

L

E

R

K

P

G

G

G

S

-

G

-

Q

T

V

I

I

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

G

A

K

A

L

S

K

R

G

G

Y

Y

K

R

V

L

I

I

I

L

V

T

M

M

P

P

E

A

T

Q

M

M

S

S

V

E

E

E

R

R

R

K

N

R

M

V

I

L

K

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

D

D

G

P

T

E

K

R

G

R

M

M

K

L

G

A

A

A

I

R

E

E

K

E

A

A

Y

L

E

R

I

L

A

-

K

K

N

E

K

E

R

L

G

G

Y

A

Y

F

I

M

P

P

Q

D

Q
|
Q

F

F

I

K

N

N

K

P

A

A

N

N

P

V

K

R

K

A

H

H

Y

Y

E

E

T

T

A

G

E

P

E

E

I

L

L

Y

E

E

D

A

L

L

Q

E

-

G

H

R

V

I

D

D

A

A

F

F

V

V

A
x
Y

G

G

V

S

G
|
G

T
|
T

A
x
G

G

G

T
|
T

L

I

A

T

G

G

V

V

G

G

E

R

N

Y

L

L

K

K

G

E

R

R

D

I

K

P

N

H

V

V

K

K

I

V

I

I

A

A

V

V

E
|
E

P

P

A

A

S

R

S
|
S

P

N

V

V

L

L

S

S

G

G

Q

K

T

M

G

G

A

Q

H

H

K

G

I

F

Q

Q

G
|
G

I
x
M

G

G

A
x
P

G

G

F

F

V

I

P

P

D

E

I

N

Y

L

I

D

P

L

E

S

L

L

V

L

D

D

K

G

I

V

I

I

T

Q

I

V

T

W

D

E

E

E

T

D

A

A

F

F

K

P

Y

L

A

A

R

R

L

R

M

L

G

A

K

R

E

E

G

G

I

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

N

I

I

V

A

W

A

A

I

L

Q

Q

V

V

A

A

E

R

E

E

L

L

G

G

K

P

G

G

K

K

K

R

V

V

V

A

T

C

V

I

A

S

P
|
P

D
|
D

G

G

E

W

K

K

Y

Y

L

L

S

S

M

T

G

P

L

L

Y

Y

active site: K40 (= K43), S70 (= S71), E200 (= E201), S204 (= S205), S263 (= S264)
binding 5-oxohexanoic acid: T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q143), G175 (= G176), G219 (= G220), M220 (≠ I221), P222 (≠ A223)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ A173), G175 (= G176), T176 (= T177), G177 (≠ A178), T179 (= T180), G219 (= G220), S263 (= S264), P289 (= P290), D290 (= D291)

2ecqA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 3-hydroxylactate
52% identity, 97% coverage: 10:302/303 of query aligns to 7:301/302 of 2ecqA

query
sites
2ecqA

I

I

G

G

A

K

T

T

P

P

L

V

F

V

K

R

L

L

D

A

N

K

F

V

K

V

N

E

K

P

D

D

S

M

A

A

E

E

I

V

Y

W

V

V

K

K

L

L

E

E

K

G

Y

L

N

N

P

P

G

G

S

S

I

I

K
|
K

D

D

R

R

A

P

A

A

L

W

G

Y

M

M

I

I

E

K

K

D

A

A

E

E

K

E

D

R

G

G

L

I

I

L

E

R

K

P

G

G

G

S

-

G

-

Q

T

V

I

I

V

V

E

E

P

P

T

T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

G

A

K

A

L

S

K

R

G

G

Y

Y

K

R

V

L

I

I

I

L

V

T

M

M

P

P

E

A

T

Q

M

M

S

S

V

E

E

E

R

R

R

K

N

R

M

V

I

L

K

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

D

D

G

P

T

E

K

R

G

R

M

M

K

L

G

A

A

A

I

R

E

E

K

E

A

A

Y

L

E

R

I

L

A

-

K

K

N

E

K

E

R

L

G

G

Y

A

Y

F

I

M

P

P

Q

D

Q
|
Q

F

F

I

K

N

N

K

P

A

A

N

N

P

V

K

R

K

A

H

H

Y

Y

E

E

T

T

A

G

E

P

E

E

I

L

L

Y

E

E

D

A

L

L

Q

E

-

G

H

R

V

I

D

D

A

A

F

F

V

V

A
x
Y

G
|
G

V

S

G
|
G

T
|
T

A

G

G

G

T
|
T

L

I

A

T

G

G

V

V

G

G

E

R

N

Y

L

L

K

K

G

E

R

R

D

I

K

P

N

H

V

V

K

K

I

V

I

I

A

A

V

V

E
|
E

P

P

A

A

S

R

S
|
S

P

N

V

V

L

L

S

S

G

G

Q

K

T

M

G

G

A

Q

H

H

K

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

V

I

P

P

D

E

I

N

Y

L

I

D

P

L

E

S

L

L

V

L

D

D

K

G

I

V

I

I

T

Q

I

V

T

W

D

E

E

E

T

D

A

A

F

F

K

P

Y

L

A

A

R

R

L

R

M

L

G

A

K

R

E

E

G

G

I

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

N

I

I

V

A

W

A

A

I

L

Q

Q

V

V

A

A

E

R

E

E

L

L

G

G

K

P

G

G

K

K

K

R

V

V

V

A

T

C

V

I

A

S

P
|
P

D

D

G

G

E

W

K

K

Y

Y

L

L

S

S

M

T

G

P

L

L

Y

Y

active site: K40 (= K43), S70 (= S71), E200 (= E201), S204 (= S205), S263 (= S264)
binding (3s)-3-hydroxybutanoic acid: K40 (= K43), G71 (= G72), T73 (= T74), Q141 (= Q143), G219 (= G220)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ A173), G173 (= G174), G175 (= G176), T176 (= T177), T179 (= T180), G219 (= G220), S263 (= S264), P289 (= P290)

2ecoA Crystal structure of t.Th. Hb8 o-acetylserine sulfhydrylase complexed with 4-methylvalerate
52% identity, 97% coverage: 10:302/303 of query aligns to 7:301/302 of 2ecoA

query
sites
2ecoA

I

I

G

G

A

K

T

T

P

P

L

V

F

V

K

R

L

L

D

A

N

K

F

V

K

V

N

E

K

P

D

D

S

M

A

A

E

E

I

V

Y

W

V

V

K

K

L

L

E

E

K

G

Y

L

N

N

P

P

G

G

S

S

I

I

K
|
K

D

D

R

R

A

P

A

A

L

W

G

Y

M

M

I

I

E

K

K

D

A

A

E

E

K

E

D

R

G

G

L

I

I

L

E

R

K

P

G

G

G

S

-

G

-

Q

T

V

I

I

V

V

E

E

P

P

T
|
T

S
|
S

G
|
G

N
|
N

T
|
T

G

G

I

I

A

G

L

L

A

A

M

M

I

I

G

A

K

A

L

S

K

R

G

G

Y

Y

K

R

V

L

I

I

I

L

V

T

M

M

P

P

E

A

T

Q

M

M

S

S

V

E

E

E

R

R

R

K

N

R

M

V

I

L

K

K

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

D

D

G

P

T

E

K

R

G

R

M

M

K

L

G

A

A

A

I

R

E

E

K

E

A

A

Y

L

E

R

I

L

A

-

K

K

N

E

K

E

R

L

G

G

Y

A

Y

F

I

M

P

P

Q

D

Q
|
Q

F

F

I

K

N

N

K

P

A

A

N

N

P

V

K

R

K

A

H

H

Y

Y

E

E

T

T

A

G

E

P

E

E

I

L

L

Y

E

E

D

A

L

L

Q

E

-

G

H

R

V

I

D

D

A

A

F

F

V

V

A
x
Y

G

G

V

S

G
|
G

T
|
T

A

G

G

G

T
|
T

L

I

A

T

G

G

V

V

G

G

E

R

N

Y

L

L

K

K

G

E

R

R

D

I

K

P

N

H

V

V

K

K

I

V

I

I

A

A

V

V

E
|
E

P

P

A

A

S

R

S
|
S

P

N

V

V

L

L

S

S

G

G

Q

K

T

M

G

G

A

Q

H

H

K

G

I

F

Q

Q

G
|
G

I

M

G

G

A

P

G

G

F

F

V

I

P

P

D

E

I

N

Y

L

I

D

P

L

E

S

L

L

V

L

D

D

K

G

I

V

I

I

T

Q

I

V

T

W

D

E

E

E

T

D

A

A

F

F

K

P

Y

L

A

A

R

R

L

R

M

L

G

A

K

R

E

E

G

G

I

L

L

F

V

L

G

G

I

M

S
|
S

S

S

G

G

A

G

N

I

I

V

A

W

A

A

I

L

Q

Q

V

V

A

A

E

R

E

E

L

L

G

G

K

P

G

G

K

K

K

R

V

V

V

A

T

C

V

I

A

S

P
|
P

D
|
D

G

G

E

W

K

K

Y

Y

L

L

S

S

M

T

G

P

L

L

Y

Y

active site: K40 (= K43), S70 (= S71), E200 (= E201), S204 (= S205), S263 (= S264)
binding 4-methyl valeric acid: K40 (= K43), T69 (= T70), G71 (= G72), T73 (= T74), Q141 (= Q143), G175 (= G176), T176 (= T177), G219 (= G220)
binding pyridoxal-5'-phosphate: K40 (= K43), N72 (= N73), Y172 (≠ A173), G175 (= G176), T176 (= T177), T179 (= T180), G219 (= G220), S263 (= S264), P289 (= P290), D290 (= D291)

8b9wA Cysteine synthase from trypanosoma theileri with plp bound (see paper)
51% identity, 97% coverage: 9:302/303 of query aligns to 16:311/329 of 8b9wA

query
sites
8b9wA

M

L

I

I

G

G

A

N

T

T

P

P

L

A

F

V

K

Y

L

L

D

R

N

N

F

M

K

-

N

N

K

K

D

T

S

A

A

A

E

T

I

I

Y

V

V

L

K

K

L

L

E

E

K

S

Y

E

N

N

P

P

G

M

G

A

S

S

I

V

K
|
K

D

D

R

R

A

L

A

A

L

Y

G

A

M

I

I

Y

E

D

K

K

A

A

E

E

K

K

D

E

G

G

L

K

I

I

E

K

K

R

G

G

-

K

G

S

T

V

I

I

V

V

E

E

P

A

T

T

S

S

G

G

N
|
N

T

T

G

G

I

I

A

A

L

L

A

A

M

L

I

L

G

G

K

A

L

V

K

R

G

G

Y

Y

K

K

V

V

I

I

V

T

M

M

P

P

E

D

T

S

M

M

S

S

V

M

E

E

R

R

N

S

M

L

I

L

K

R

A

I

Y

F

G

G

A

A

E

E

L

L

V

I

L

L

T

T

D

P

G

A

T

S

K

L

G

G

M

M

K

K

G

G

A

A

I

V

E

A

K

M

A

A

Y

N

E

R

I

I

A

V

K

S

N

N

K

N

R

P

G

D

Y

A

Y

V

I

L

P

A

Q

D

Q

Q

F

F

I

C

N

T

K

K

A

Y

N

N

P

A

K

Q

K

I

H

H

Y

E

E

E

T

T

A

G

E

P

E

E

I

I

L

W

E

R

D

Q

L

T

Q

K

-

G

H

H

V

V

D

D

A

C

F

F

V

V

A

A

G

G

V

V

G
|
G

T
|
T

A
x
G

G

G

T
|
T

L

L

A

T

G

G

V

V

G

A

E

R

N

F

L

L

K

K

G

S

R

V

D

G

K

C

N

N

V

A

K

N

I

I

I

V

A

A

V

V

E

E

P

P

A

Q

S

E

S

S

P

P

V

V

L

L

S

S

G

G

Q

R

T

P

G

G

A

P

H

H

K

R

I

I

Q

Q

G
|
G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

D

E

I

V

Y

L

I

D

P

R

E

E

L

L

V

V

D

D

K

E

I

I

T

Q

I

V

T

S

D

S

E

D

T

K

A

A

F

I

K

E

Y

T

A

A

R

Q

L

K

M

L

G

S

K

R

E

M

E

D

G

G

I

I

L

F

V

C

G

G

I

F

S
|
S

S

A

G

G

A

A

N

N

I

V

A

F

A

A

I

L

Q

K

V

I

A

A

E

E

-

R

-

P

E

E

L

M

G

A

K

-

G

G

K

K

K

T

V

I

V

V

T

T

V

V

A

I

P
|
P

D

S

G

F

G

G

E

E

K

R

Y

Y

L

L

S

S

M

T

G

A

L

L

Y

Y

binding pyridoxal-5'-phosphate: K49 (= K43), N80 (= N73), G184 (= G176), T185 (= T177), G186 (≠ A178), T188 (= T180), G228 (= G220), S272 (= S264), P299 (= P290)

P47999 Cysteine synthase, chloroplastic/chromoplastic; At.OAS.7-4; Beta-substituted Ala synthase 2;1; ARAth-Bsas2;1; CSase B; AtCS-B; CS-B; O-acetylserine (thiol)-lyase; O-acetylserine sulfhydrylase; OAS-TL B; cpACS1; EC 2.5.1.47 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
51% identity, 100% coverage: 2:303/303 of query aligns to 75:379/392 of P47999

query
sites
P47999

I

I

F

A

N

D

N

N

A

A

I

A

D

Q

M

L

I

I

G

G

A

K

T

T

P

P

L

M

F

V

K

Y

L

L

D

N

N

N

F

V

K

V

N

K

K

G

D

C

S

V

A

A

E

S

I

V

Y

A

V

A

K

K

L

L

E

E

K

I

Y

M

N

E

P

P

G

C

S

S

I

V

K

K

D

D

R

R

A

I

A

G

L

Y

G

S

M

M

I

I

E

T

K

D

A

A

E

E

K

E

D

K

G

G

L

L

I

I

E

T

K

P

G

G

G

K

T

S

I

V

-

L

V

V

E

E

P

S

T

T

S

S

G

G

N

N

T

T

G

G

I

I

A

G

L

L

A

A

M

F

I

I

G

A

K

A

L

S

K

K

G

G

Y

Y

K

K

V

L

I

I

I

L

V

T

M

M

P

P

E

A

T

S

M

M

S

S

V

L

E

E

R

R

N

V

M

L

I

L

K

R

A

A

Y

F

G

G

A

A

E

E

L

L

V

V

L

L

T

T

D

E

G

P

T

A

K

K

G

G

M

M

K

T

G

G

A

A

I

I

E

Q

K

K

A

A

Y

E

E

E

I

I

A

L

K

K

N

K

K

T

R

P

G

N

Y

S

Y

Y

I

M

P

L

Q

Q

F

F

I

D

N

N

K

P

A

A

N

N

P

P

K

K

K

I

H

H

Y

Y

E

E

T

T

A

G

E

P

E

E

I

I

L

W

E

E

D

D

L

T

Q

R

-

G

H

K

V

I

D

D

A

I

F

L

V

V

A

A

G

G

V

I

G

G

T

T

A

G

G

G

T

T

L

I

A

T

G

G

V

V

G

G

E

R

N

F

L

I

K

K

G

E

R

R

D

K

K

P

N

E

V

L

K

K

I

V

I

I

A

G

V

V

E

E

P

P

A

T

S

E

S

S

P

A

V

I

L

L

S

S

G

G

Q

K

T

P

G

G

A

P

H

H

K

K

I

I

Q

Q

G

G

I

I

G

G

A

A

G

G

F

F

V

V

P

P

D

K

I

N

Y

L

I

D

P

L

E

A

L

I

V

V

D

D

K

E

I

Y

I

I

T

A

I

I

T

S

D

S

E

E

T

E

A

A

F

I

K

E

Y

T

A

S

R

K

L

Q

M

L

G

A

K

L

E

Q

E

E

G

G

I

L

L

L

V

V

G

G

I

I

S

S

G

G

A

A

N

A

I

A

A

A

I

I

Q

Q

V

V

A

A

E

K

E

R

L

P

-

E

G

N

K

A

G

G

K

K

K

L

V

I

V

A

T

V

V

V

A

F

P

P

D

S

G

F

G

G

E

E

K

R

Y

Y

L

L

S

S

M

T

G

Q

L

L

Y

F

D

Q

Sites not aligning to the query:

61 modified: N-acetylalanine

6kr5B Crystal structure of o-acetyl serine sulfhydrylase isoform 3 from entamoeba histolytica (see paper)
51% identity, 99% coverage: 2:302/303 of query aligns to 11:317/336 of 6kr5B

query
sites
6kr5B

I

I

F

Y

N

H

N

N

A

I

I

L

D

E

M

T

I

I

G

G

A

G

T

T

P

P

L

L

F

V

K

E

L

L

-

H

-

G

-

V

-

T

D

D

N

H

F

P

K

S

N

I

K

K

D

K

S

N

A

T

E

K

I

I

Y

L

V

V

K

K

L

L

E

E

K

C

Y

F

N

N

P

P

G

M

G

S

S

S

I

V

K
|
K

D

D

R

R

A

V

A

G

L

F

G

N

M

I

I

I

E

Y

K

Q

A

A

E

I

K

K

D

D

G

G

L

R

I

L

E

K

K

P

G

G

G

M

T

E