SitesBLAST
Comparing WP_012113724.1 NCBI__GCF_000017645.1:WP_012113724.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
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Found 20 (the maximum) hits to proteins with known functional sites (download)
6wy8C Tcur3481-tcur3483 steroid acad (see paper)
31% identity, 97% coverage: 9:351/354 of query aligns to 7:355/364 of 6wy8C
Sites not aligning to the query:
6wy9B Tcur3481-tcur3483 steroid acad g363a variant (see paper)
31% identity, 97% coverage: 9:351/354 of query aligns to 4:352/361 of 6wy9B
Sites not aligning to the query:
6fahD Molecular basis of the flavin-based electron-bifurcating caffeyl-coa reductase reaction (see paper)
24% identity, 95% coverage: 9:344/354 of query aligns to 5:366/379 of 6fahD
- active site: L124 (vs. gap), T125 (vs. gap), G241 (≠ A218)
- binding flavin-adenine dinucleotide: F122 (≠ Y118), L124 (vs. gap), T125 (vs. gap), R152 (= R136), F155 (≠ L139), T157 (vs. gap), E198 (vs. gap), R267 (= R244), Q269 (= Q246), F270 (= F247), I274 (≠ L251), F277 (= F254), Q335 (= Q313), I336 (≠ V314), G339 (= G317), Y361 (≠ L339), T364 (≠ S342), Q366 (= Q344)
Sites not aligning to the query:
4l1fA Electron transferring flavoprotein of acidaminococcus fermentans: towards a mechanism of flavin-based electron bifurcation (see paper)
27% identity, 89% coverage: 9:324/354 of query aligns to 7:347/380 of 4l1fA
- active site: L125 (= L116), T126 (≠ A117), G242 (≠ A218)
- binding coenzyme a persulfide: T132 (≠ L124), H179 (vs. gap), F232 (= F208), M236 (≠ A212), E237 (≠ L213), L239 (≠ A215), D240 (= D216), R243 (≠ A219)
- binding flavin-adenine dinucleotide: F123 (≠ V114), L125 (= L116), T126 (≠ A117), G131 (= G123), T132 (≠ L124), F156 (≠ L139), I157 (≠ A140), T158 (≠ L141), R268 (= R244), Q270 (= Q246), F271 (= F247), I275 (≠ L251), F278 (= F254), L281 (≠ V257), Q336 (= Q313), I337 (≠ V314), G340 (= G317)
- binding 1,3-propandiol: Q10 (≠ A12)
Sites not aligning to the query:
5ol2F The electron transferring flavoprotein/butyryl-coa dehydrogenase complex from clostridium difficile (see paper)
24% identity, 82% coverage: 36:326/354 of query aligns to 37:348/378 of 5ol2F
- active site: L124 (= L116), T125 (≠ A117), G241 (≠ A218)
- binding coenzyme a persulfide: L238 (≠ A215), R242 (≠ A219)
- binding flavin-adenine dinucleotide: F122 (≠ V114), L124 (= L116), T125 (≠ A117), P127 (= P120), T131 (≠ L124), F155 (vs. gap), I156 (vs. gap), T157 (vs. gap), E198 (≠ D175), R267 (= R244), F270 (= F247), L274 (= L251), F277 (= F254), Q335 (= Q313), L336 (≠ V314), G338 (= G316), G339 (= G317)
Sites not aligning to the query:
8i4rA Crystal structure of acyl-coa dehydrogenase complexed with acetyl-coa from thermobifida fusca
24% identity, 95% coverage: 9:344/354 of query aligns to 8:369/381 of 8i4rA
- binding acetyl coenzyme *a: S132 (≠ T131), T134 (vs. gap), R180 (≠ A158), R234 (≠ E209), L237 (≠ A212), R238 (≠ L213), L240 (≠ A215), D241 (= D216), R244 (≠ A219), E365 (≠ F340), G366 (= G341)
- binding flavin-adenine dinucleotide: Y123 (= Y118), L125 (= L124), S126 (≠ R125), G131 (= G130), S132 (≠ T131), W156 (≠ L139), I157 (≠ A140), T158 (≠ L141), I360 (≠ T335), T367 (≠ S342), Q369 (= Q344)
Sites not aligning to the query:
8i4pA Crystal structure of acyl-coa dehydrogenase from thermobifida fusca
24% identity, 95% coverage: 9:344/354 of query aligns to 8:369/381 of 8i4pA
- binding flavin-adenine dinucleotide: Y123 (= Y118), L125 (= L124), S126 (≠ R125), G131 (= G130), S132 (≠ T131), W156 (≠ L139), I157 (≠ A140), T158 (≠ L141), I360 (≠ T335), Y364 (≠ L339), T367 (≠ S342), Q369 (= Q344)
7w0jE Acyl-coa dehydrogenase, tfu_1647
24% identity, 95% coverage: 9:344/354 of query aligns to 9:370/382 of 7w0jE
- binding [[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2R,3S,4S)-5-azanyl-2,3,4-tris(oxidanyl)pentyl] hydrogen phosphate: S127 (≠ R125), W157 (≠ L139), R270 (= R244), Q272 (= Q246), F273 (= F247), I277 (≠ L251), F280 (= F254), I283 (≠ V257), Q339 (= Q313), L340 (≠ V314), G343 (= G317), Y365 (≠ L339), E366 (≠ F340), T368 (≠ S342), Q370 (= Q344)
Sites not aligning to the query:
3pfdC Crystal structure of an acyl-coa dehydrogenase from mycobacterium thermoresistibile bound to reduced flavin adenine dinucleotide solved by combined iodide ion sad mr (see paper)
26% identity, 88% coverage: 35:344/354 of query aligns to 24:357/369 of 3pfdC
- active site: L116 (= L124), S117 (≠ R125), T233 (≠ A218), E353 (≠ F340)
- binding dihydroflavine-adenine dinucleotide: Y114 (= Y118), L116 (= L124), S117 (≠ R125), G122 (= G130), S123 (≠ T131), W147 (= W149), I148 (= I150), T149 (≠ V151), R259 (= R244), F262 (= F247), V266 (≠ L251), N269 (≠ F254), Q326 (= Q313), L327 (≠ V314), G330 (= G317), I348 (≠ T335), Y352 (≠ L339), T355 (≠ S342), Q357 (= Q344)
Sites not aligning to the query:
P96855 Acyl-CoA dehydrogenase FadE34; ACAD; 3-oxochol-4-en-24-oyl-CoA dehydrogenase; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
31% identity, 90% coverage: 14:332/354 of query aligns to 33:324/711 of P96855
- R236 (= R244) mutation to A: Displays less than 2% activity with cholyl-CoA as substrate. Cannot bind FAD.
Sites not aligning to the query:
- 581 E→Q: Displays less than 1% activity with cholyl-CoA as substrate. Still binds FAD.
4x28D Crystal structure of the chse4-chse5 complex from mycobacterium tuberculosis (see paper)
28% identity, 98% coverage: 5:351/354 of query aligns to 27:356/364 of 4x28D
Sites not aligning to the query:
2vigB Crystal structure of human short-chain acyl coa dehydrogenase
26% identity, 90% coverage: 15:331/354 of query aligns to 13:352/371 of 2vigB
- active site: L121 (= L116), S122 (≠ A117), G231 (≠ A218), E352 (≠ K331)
- binding coenzyme a persulfide: S128 (≠ L124), F221 (= F208), M225 (≠ A212), Q226 (≠ L213), L228 (≠ A215), D229 (= D216), R232 (≠ A219), E352 (≠ K331)
- binding flavin-adenine dinucleotide: L121 (= L116), S122 (≠ A117), G127 (= G123), S128 (≠ L124), W152 (= W149), T154 (≠ V151), R257 (= R244), F260 (= F247), L264 (= L251), L267 (≠ F254), Q325 (= Q313), I326 (≠ V314), G329 (= G317), I347 (= I326), Y351 (= Y330)
Sites not aligning to the query:
I6Y3Q0 Acyl-CoA dehydrogenase FadE27; ACAD; 3-oxocholest-4-en-26-oyl-CoA dehydrogenase beta subunit; EC 1.3.99.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
29% identity, 98% coverage: 5:351/354 of query aligns to 27:365/373 of I6Y3Q0