SitesBLAST
Comparing WP_012114601.1 NCBI__GCF_000017645.1:WP_012114601.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.
Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures
Found 20 (the maximum) hits to proteins with known functional sites (download)
P69874 Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11 from Escherichia coli (strain K12) (see 3 papers)
49% identity, 85% coverage: 17:332/372 of query aligns to 18:337/378 of P69874
- C26 (≠ R25) mutation to A: Lower ATPase activity and transport efficiency.
- F27 (= F26) mutation to L: Lower ATPase activity and transport efficiency.
- F45 (= F44) mutation to L: Lower ATPase activity and transport efficiency.
- C54 (= C53) mutation to T: Loss of ATPase activity and transport.
- L60 (= L59) mutation to F: Lower ATPase activity and transport efficiency.
- L76 (≠ V75) mutation to P: Lower ATPase activity and transport efficiency.
- V135 (= V134) mutation to M: Loss of ATPase activity and transport.
- D172 (= D171) mutation to N: Loss of ATPase activity and transport.
- C276 (≠ A272) mutation to A: Lower ATPase activity and transport efficiency.
- E297 (≠ D297) mutation E->K,D: Lower ATPase activity and transport efficiency.; mutation to Q: Loss of ATPase activity and transport.
2d62A Crystal structure of multiple sugar binding transport atp- binding protein
42% identity, 95% coverage: 17:370/372 of query aligns to 7:359/375 of 2d62A
1g291 Malk (see paper)
54% identity, 63% coverage: 16:248/372 of query aligns to 3:241/372 of 1g291
- binding magnesium ion: D69 (≠ E82), E71 (vs. gap), K72 (vs. gap), K79 (≠ Y86), D80 (= D87)
- binding pyrophosphate 2-: S38 (= S51), G39 (= G52), C40 (= C53), G41 (= G54), K42 (= K55), T43 (= T56), T44 (= T57)
Sites not aligning to the query:
3puyA Crystal structure of an outward-facing mbp-maltose transporter complex bound to amp-pnp after crystal soaking of the pretranslocation state (see paper)
41% identity, 86% coverage: 16:336/372 of query aligns to 2:327/371 of 3puyA
- binding phosphoaminophosphonic acid-adenylate ester: W12 (≠ F26), S37 (= S51), G38 (= G52), C39 (= C53), G40 (= G54), K41 (= K55), S42 (≠ T56), T43 (= T57), Q81 (= Q95), R128 (= R142), A132 (≠ Q146), S134 (= S148), G136 (= G150), Q137 (= Q151), E158 (= E172), H191 (= H205)
- binding magnesium ion: S42 (≠ T56), Q81 (= Q95)
3puxA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-bef3 (see paper)
41% identity, 86% coverage: 16:336/372 of query aligns to 2:327/371 of 3puxA
- binding adenosine-5'-diphosphate: W12 (≠ F26), G38 (= G52), C39 (= C53), G40 (= G54), K41 (= K55), S42 (≠ T56), T43 (= T57), R128 (= R142), S134 (= S148), Q137 (= Q151)
- binding beryllium trifluoride ion: S37 (= S51), G38 (= G52), K41 (= K55), Q81 (= Q95), S134 (= S148), G136 (= G150), H191 (= H205)
- binding magnesium ion: S42 (≠ T56), Q81 (= Q95)
3puwA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-alf4 (see paper)
41% identity, 86% coverage: 16:336/372 of query aligns to 2:327/371 of 3puwA
- binding adenosine-5'-diphosphate: W12 (≠ F26), V17 (≠ A31), G38 (= G52), C39 (= C53), G40 (= G54), K41 (= K55), S42 (≠ T56), T43 (= T57), R128 (= R142), A132 (≠ Q146), S134 (= S148), Q137 (= Q151)
- binding tetrafluoroaluminate ion: S37 (= S51), G38 (= G52), K41 (= K55), Q81 (= Q95), S134 (= S148), G135 (= G149), G136 (= G150), E158 (= E172), H191 (= H205)
- binding magnesium ion: S42 (≠ T56), Q81 (= Q95)
3puvA Crystal structure of an outward-facing mbp-maltose transporter complex bound to adp-vo4 (see paper)
41% identity, 86% coverage: 16:336/372 of query aligns to 2:327/371 of 3puvA
- binding adenosine-5'-diphosphate: W12 (≠ F26), V17 (≠ A31), G38 (= G52), C39 (= C53), G40 (= G54), K41 (= K55), S42 (≠ T56), T43 (= T57), R128 (= R142), A132 (≠ Q146), S134 (= S148), Q137 (= Q151)
- binding magnesium ion: S42 (≠ T56), Q81 (= Q95)
P68187 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Escherichia coli (strain K12) (see 5 papers)
41% identity, 86% coverage: 16:336/372 of query aligns to 3:328/371 of P68187
- A85 (= A98) mutation to M: Suppressor of EAA loop mutations in MalFG.
- K106 (≠ P119) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V114 (= V127) mutation to C: Suppressor of EAA loop mutations in MalFG.
- V117 (≠ A130) mutation to M: Suppressor of EAA loop mutations in MalFG.
- E119 (≠ A132) mutation to K: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- A124 (= A137) mutation to T: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G137 (= G150) mutation to A: Loss of maltose transport. Has greater ability to decrease mal gene expression than wild-type MalK.
- D158 (= D171) mutation to N: Loss of maltose transport but retains ability to repress mal genes.
- R228 (≠ P241) mutation to C: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- F241 (≠ V252) mutation to I: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- W267 (≠ Q275) mutation to G: Normal maltose transport but constitutive mal gene expression.
- G278 (= G287) mutation to P: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- S282 (≠ D291) mutation to L: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G284 (≠ F293) mutation to S: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- G302 (= G310) mutation to D: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
- E308 (≠ V316) mutation to Q: Maltose transport is affected but retains ability to interact with MalT.
- S322 (≠ A330) mutation to F: Resistant to inhibitory effects of alpha-methylglucoside but retains transport capacity.
Sites not aligning to the query:
- 340 G→A: Maltose transport is affected but retains ability to interact with MalT.
- 346 G→S: Normal maltose transport but constitutive mal gene expression.
- 355 F→Y: Maltose transport is affected but retains ability to interact with MalT.
2awnB Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
41% identity, 86% coverage: 16:336/372 of query aligns to 2:327/374 of 2awnB
1q12A Crystal structure of the atp-bound e. Coli malk (see paper)
41% identity, 86% coverage: 17:336/372 of query aligns to 1:325/367 of 1q12A
- binding adenosine-5'-triphosphate: W10 (≠ F26), S35 (= S51), G36 (= G52), C37 (= C53), G38 (= G54), K39 (= K55), S40 (≠ T56), T41 (= T57), R126 (= R142), A130 (≠ Q146), S132 (= S148), G134 (= G150), Q135 (= Q151)
P9WQI3 Trehalose import ATP-binding protein SugC; MtbSugC; Nucleotide-binding domain of SugABC transporter; NBD of SugABC transporter; SugABC transporter ATPase SugC; EC 7.5.2.- from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (see paper)
48% identity, 65% coverage: 17:257/372 of query aligns to 4:247/393 of P9WQI3
- H193 (= H205) mutation to A: Decreased hydrolysis of ATP. No change in KM, but 2-fold reduction in Vmax compared to wild-type.
P19566 Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720) (see paper)
40% identity, 86% coverage: 17:336/372 of query aligns to 4:326/369 of P19566
- L86 (= L99) mutation to F: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- P160 (= P173) mutation to L: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- D165 (= D178) mutation to N: Loss of transport. No effect on ATP-binding activity but decrease in ATP hydrolysis. Retains repressor activity.
- E306 (≠ V316) mutation to K: Loss of transport. No effect on ATP-binding and ATP hydrolysis. Retains repressor activity.
8hprC Lpqy-sugabc in state 4 (see paper)
48% identity, 64% coverage: 17:255/372 of query aligns to 3:244/363 of 8hprC
- binding adenosine-5'-triphosphate: Y12 (≠ F26), S38 (= S51), G39 (= G52), G41 (= G54), K42 (= K55), S43 (≠ T56), Q82 (= Q95), Q133 (= Q146), G136 (= G149), G137 (= G150), Q138 (= Q151), H192 (= H205)
- binding magnesium ion: S43 (≠ T56), Q82 (= Q95)
8hprD Lpqy-sugabc in state 4 (see paper)
48% identity, 64% coverage: 17:255/372 of query aligns to 3:244/362 of 8hprD
- binding adenosine-5'-triphosphate: Y12 (≠ F26), S38 (= S51), C40 (= C53), G41 (= G54), K42 (= K55), S43 (≠ T56), T44 (= T57), Q82 (= Q95), R129 (= R142), Q133 (= Q146), S135 (= S148), G136 (= G149), G137 (= G150), Q159 (≠ E172), H192 (= H205)
- binding magnesium ion: S43 (≠ T56), Q82 (= Q95)
8hplC Lpqy-sugabc in state 1 (see paper)
48% identity, 64% coverage: 17:255/372 of query aligns to 3:242/384 of 8hplC
1vciA Crystal structure of the atp-binding cassette of multisugar transporter from pyrococcus horikoshii ot3 complexed with atp (see paper)
50% identity, 64% coverage: 15:253/372 of query aligns to 5:237/353 of 1vciA
2awnC Crystal structure of the adp-mg-bound e. Coli malk (crystallized with atp-mg) (see paper)
38% identity, 84% coverage: 25:336/372 of query aligns to 4:297/344 of 2awnC
3d31A Modbc from methanosarcina acetivorans (see paper)
39% identity, 84% coverage: 17:328/372 of query aligns to 2:311/348 of 3d31A
Sites not aligning to the query:
7ahhC Opua inhibited inward-facing, sbd docked (see paper)
43% identity, 61% coverage: 22:249/372 of query aligns to 32:265/382 of 7ahhC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
- binding phosphoaminophosphonic acid-adenylate ester: 12
7aheC Opua inhibited inward facing (see paper)
43% identity, 61% coverage: 22:249/372 of query aligns to 32:265/382 of 7aheC
Sites not aligning to the query:
- binding (2R,3R,3aS,5R,7aR,9R,10R,10aS,12R,14aR)-2,9-bis(6-amino-9H-purin-9-yl)octahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide: 275, 297, 298
Query Sequence
>WP_012114601.1 NCBI__GCF_000017645.1:WP_012114601.1
MSSIPPDAASRTRPVGVQIDGVTKRFGDATALHEAWLKIGQGEFMTLLGPSGCGKTTLLN
LIAGFLEADGGEIFVSGSLMTETPPYDREIGVVFQNYALFPHMSVAKNVGYGLRMRGVPK
REIEQRVAEALALVKLAAFGDRKPRQLSGGQQQRVALARALVIRPKVLLLDEPFSALDKN
LRGSMQLELKEIQRKLGVTTIFVTHDQSEALSMSDRIAVMSDGRIRQVGTPDEIYRRPLD
PFVAAFVGDVNVLPARLERRLGDMATVVLGEARVQVRAQTLDGLAPGAAVDLFVRPDAMR
FAEAEGVLSGTVATHVYQGSHVDVLVDVPAAKGGRVIVRVPGHEAPGRWPAGARVTLDFA
AGDAVAFASDAN
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SitesBLAST's Database
SitesBLAST's database includes
(1) SwissProt
entries with experimentally-supported functional features;
and (2) protein structures with bound ligands, from the
BioLip database.
by Morgan Price,
Arkin group
Lawrence Berkeley National Laboratory