SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012115496.1 NCBI__GCF_000017645.1:WP_012115496.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q5F8J4 Homoserine dehydrogenase; HDH; HSD; NAD(+)-dependent homoserine dehydrogenase; NAD(+)-dependent HSD; NgHSD; EC 1.1.1.3 from Neisseria gonorrhoeae (strain ATCC 700825 / FA 1090) (see paper)
43% identity, 93% coverage: 28:426/428 of query aligns to 28:431/435 of Q5F8J4

query
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Q5F8J4

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L45 (≠ R45) mutation to R: Shows a marked increase in the catalytic efficiency with NADP(+).
LS 45:46 (≠ RD 45:46) mutation to RD: Does not impair the catalytic activity with NAD(+). Slightly increases the activity, but slightly decreases the affinity for NADP(+).; mutation to RR: Causes a shift in coenzyme preference from NAD(+) to NADP(+) by a factor of 974. Shows a slight decrease in the catalytic efficiency with NAD(+) and a 4.5-fold increase in catalytic efficiency with NADP(+).

6dzsA Mycobacterial homoserine dehydrogenase thra in complex with NADP
40% identity, 99% coverage: 4:426/428 of query aligns to 5:429/431 of 6dzsA

query
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binding nadp nicotinamide-adenine-dinucleotide phosphate: G11 (= G10), L12 (= L11), G13 (= G12), N14 (≠ T13), V15 (= V14), V45 (≠ A44), R46 (= R45), R47 (≠ D46), R52 (= R51), I63 (≠ V65), L78 (= L79), M79 (= M80), P84 (= P86), A102 (= A104), K104 (= K106), G306 (= G302), T310 (= T306)

4pg7A Crystal structure of s. Aureus homoserine dehydrogenase at ph7.5 (see paper)
38% identity, 74% coverage: 1:318/428 of query aligns to 2:307/402 of 4pg7A

query
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4pg7A

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active site: D190 (= D202), K194 (= K206)
binding lysine: R215 (≠ E227), G219 (≠ Q231)

Sites not aligning to the query:

binding lysine: 394, 395

6a0tB Homoserine dehydrogenase k99a mutant from thermus thermophilus hb8 complexed with hse and NADP+ (see paper)
37% identity, 67% coverage: 1:287/428 of query aligns to 1:274/332 of 6a0tB

query
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6a0tB

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G

G

D

V

G

E

D

A

P

P

A

L

K

R

A

L

A

-

V

V

A

L

A

P

A

A

L

L

D

E

R

A

G

G

I

I

P

P

V

L

V

I

T

T

A
|
A

N
|
N

K

K

A

A

L

L

A

A

K

E

C

A

G

W

V

E

E

S

L

L

A

-

Q

-

R

R

A

P

E

F

A

A

S

E

R

E

A

G

G

L

L

I

H

Y

F

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

V

L

K

S

T

F

L

L

R

-

E

E

S

T

L

L

A

R

G

G

N

S

T

E

I

L

E

L

R

E

V

L

S

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

A

-

E

E

E

K

K

G

L

R

S

T

F

Y

H

A

V

E

C

A

L

L

G

L

E

E

A

A

Q

Q

R

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

I

V

D

E

G

G

F

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

T

A

S

R

L

L

A

L

F

V

G

D

T

P

Q

G

V

F

D

P

A

F

D

A

S

E

I

V

Y

E

V

A

E

Q

G

G

I

I

R

A

Q

R

L

L

T

T

L

P

A

E

D

V

L

L

E

Q

A

K

A

A

D

E

D

A

L

R

G

G

Y

E

R

R

V

V

K

R

L

L

L

V

G

A

V

S

A

L

V

F

R

G

T

E

E

G

T

G

G

R

I

W

E

R

Q

A

R

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

K

Q

H

D

W

H

P

P

I

L

A

A

Q

R

V

A

S

R

G

G

V

-

T

-

N

N

A

A

V

L

A

W

V

V

active site: D191 (= D202), K195 (= K206)
binding l-homoserine: N150 (= N160), G151 (= G161), T152 (= T162), Y178 (= Y189), E180 (= E191), D186 (= D197), K195 (= K206)
binding nadp nicotinamide-adenine-dinucleotide phosphate: G10 (= G10), G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (≠ A43), V43 (≠ A44), R44 (= R45), D45 (= D46), K48 (≠ R49), R50 (= R51), A73 (≠ L79), M74 (= M80), G75 (= G81), A97 (= A104), N98 (= N105), G177 (= G188), E180 (= E191)

Sites not aligning to the query:

binding nadp nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

6a0sA Homoserine dehydrogenase from thermus thermophilus hb8 complexed with hse and NADPH (see paper)
37% identity, 67% coverage: 1:287/428 of query aligns to 1:274/331 of 6a0sA

query
sites
6a0sA

M

M

S

E

E

A

L

L

K

K

V

I

G

A

L

L

A

L

G

G

L
x
G

G
|
G

T
|
T

V
|
V

G

G

A

S

A

A

V

F

F

Y

R

N

M

L

L

V

E

L

R

E

R

R

K

A

A

E

E

E

L

L

E

S

A

A

R

-

T

F

G

G

R

V

T

V

L

P

R

R

V

F

T

L

A

G

V

V

A
x
L

A
x
V

R
|
R

D
|
D

R

P

S

R

R
x
K

D

P

R
|
R

G

A

L

I

D

-

L

-

G

-

V

-

I

-

W

-

F

-

E

-

D

-

P

P

V

Q

A

E

L

L

A

L

R

R

D

A

G

E

D

P

I

F

D

D

V

L

F

L

-

E

-

A

-

D

-

L

-

V

V

V

E

E

L
x
A

M
|
M

G

G

D

V

G

E

D

A

P

P

A

L

K

R

A

L

A

-

V

V

A

L

A

P

A

A

L

L

D

E

R

A

G

G

I

I

P

P

V

L

V

I

T

T

A
|
A

N

N

K
|
K

A

A

L

L

A

A

K

E

C

A

G

W

V

E

E

S

L

L

A

-

Q

-

R

R

A

P

E

F

A

A

S

E

R

E

A

G

G

L

L

I

H

Y

F

H

E

E

A

A

A

S

V

V

A

M

G

A

G

G

I

T

P

P

I

A

V

L

K

S

T

F

L

L

R

-

E

E

S

T

L

L

A

R

G

G

N

S

T

E

I

L

E

L

R

E

V

L

S

H

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

A

-

E

E

E

K

K

G

L

R

S

T

F

Y

H

A

V

E

C

A

L

L

G

L

E

E

A

A

Q

Q

R

R

L

L

G
|
G

Y
|
Y

A

A

E
|
E

A

A

D

D

P

P

T

T

F

L

D
|
D

I

V

D

E

G

G

F

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

T

A

S

R

L

L

A

L

F

V

G

D

T

P

Q

G

V

F

D

P

A

F

D

A

S

E

I

V

Y

E

V

A

E

Q

G

G

I

I

R

A

Q

R

L

L

T

T

L

P

A

E

D

V

L

L

E

Q

A

K

A

A

D

E

D

A

L

R

G

G

Y

E

R

R

V

V

K

R

L

L

L

V

G

A

V

S

A

L

V

F

R

G

T

E

E

G

T

G

G

R

I

W

E

R

Q

A

R

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

K

Q

H

D

W

H

P

P

I

L

A

A

Q

R

V

A

S

R

G

G

V

-

T

-

N

N

A

A

V

L

A

W

V

V

active site: D191 (= D202), K195 (= K206)
binding l-homoserine: K99 (= K106), N150 (= N160), G151 (= G161), T152 (= T162), Y178 (= Y189), E180 (= E191), D186 (= D197), K195 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G11 (≠ L11), G12 (= G12), T13 (= T13), V14 (= V14), L42 (≠ A43), V43 (≠ A44), R44 (= R45), D45 (= D46), K48 (≠ R49), R50 (= R51), A73 (≠ L79), M74 (= M80), A97 (= A104), K99 (= K106), G177 (= G188), E180 (= E191)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 289, 290, 294

2ejwA Homoserine dehydrogenase from thermus thermophilus hb8
37% identity, 67% coverage: 1:287/428 of query aligns to 1:274/331 of 2ejwA

query
sites
2ejwA

M

M

S

E

E

A

L

L

K

K

V

I

G

A

L

L

A

L

G

G

L

G

G

G

T

T

V

V

G

G

A

S

A

A

V

F

F

Y

R

N

M

L

L

V

E

L

R

E

R

R

K

A

A

E

E

E

L

L

E

S

A

A

R

-

T

F

G

G

R

V

T

V

L

P

R

R

V

F

T

L

A

G

V

V

A

L

A

V

R

R

D

D

R

P

S

R

R

K

D

P

R

R

G

A

L

I

D

-

L

-

G

-

V

-

I

-

W

-

F

-

E

-

D

-

P

P

V

Q

A

E

L

L

A

L

R

R

D

A

G

E

D

P

I

F

D

D

V

L

F

L

-

E

-

A

-

D

-

L

-

V

V

V

E

E

L

A

M

M

G

G

D

V

G

E

D

A

P

P

A

L

K

R

A

L

A

-

V

V

A

L

A

P

A

A

L

L

D

E

R

A

G

G

I

I

P

P

V

L

V

I

T

T

A

A

N

N

K

K

A

A

L

L

A

A

K

E

C

A

G

W

V

E

E

S

L

L

A

-

Q

-

R

R

A

P

E

F

A

A

S

E

R

E

A

G

G

L

L

I

H

Y

F

H

E
|
E

A

A

A

S

V
|
V

A
x
M

G
x
A

G

G

I
x
T

P

P

I

A

V

L

K

S

T

F

L

L

R

-

E

E

S

T

L

L

A

R

G

G

N

S

T

E

I

L

E

L

R

E

V

L

S

H

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

L

Y

Y

I

I

L

L

T

Q

R

E

M

M

A

-

E

E

E

K

K

G

L

R

S

T

F

Y

H

A

V

E

C

A

L

L

G

L

E

E

A

A

Q

Q

R

R

L

L

G

G

Y

Y

A

A

E

E

A

A

D

D

P

P

T

T

F

L

D

D

I

V

D

E

G

G

F

I

D
|
D

T

A

A

A

H

H

K
|
K

L

L

A

T

I

L

L

L

T

A

S

R

L

L

A

L

F

V

G

D

T

P

Q

G

V

F

D

P

A

F

D

A

S

E

I

V

Y

E

V

A

E

Q

G

G

I

I

R

A

Q

R

L

L

T

T

L

P

A

E

D

V

L

L

E

Q

A

K

A

A

D

E

D

A

L

R

G

G

Y

E

R

R

V

V

K

R

L

L

L

V

G

A

V

S

A

L

V

F

R

G

T

E

E

G

T

G

G

R

I

W

E

R

Q

A

R

A

V

V

H

A

P

P

T

R

M

R

V

L

P

P

K

Q

H

D

W

H

P

P

I

L

A

A

Q

R

V

A

S

R

G

G

V

-

T

-

N

N

A

A

V

L

A

W

V

V

active site: D191 (= D202), K195 (= K206)
binding magnesium ion: E121 (= E130), V124 (= V133), M125 (≠ A134), A126 (≠ G135), T128 (≠ I137)

F9VNG5 Homoserine dehydrogenase; HDH; HSD; StHSD; EC 1.1.1.3 from Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) (Sulfolobus tokodaii) (see 3 papers)
33% identity, 74% coverage: 4:319/428 of query aligns to 1:301/304 of F9VNG5

query
sites
F9VNG5

L

M

K

K

V

L

G

L

L

L

A

F

G

G

L
x
Y

G

G

T
x
N

V
|
V

G

G

A

K

A

A

V

F

F

R

R

K

M

L

L

L

-

H

E

E

R

K

R

R

K

S

A

P

E

E

L

L

-

N

E

D

A

V

R

I

T

I

G

G

R

G

T

I

L

V

R

T

V
x
R

T
x
R

A

G

V

I

A

M

A

L

R

Q

D

D

R

K

S

-

R

E

D

D

R

F

G

T

L

P

D

D

L

L

G

E

G

G

V

D

I

V

W

F

-

K

-

A

F

F

E

E

D

-

P

-

V

-

A

K

L

I

A

K

R

P

D

D

G

I

D

I

I

V

D

D

V

V

F

-

V
x
S

E

S

L

A

M

N

G

Y

G

N

D

N

G

G

D

E

P

P

A

S

K

L

A

S

A

L

V

Y

A

K

A

E

A

A

L

I

D

K

R

D

G

G

I

V

P

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

K

L

C

A

G

F

V

N

E

E

L

I

A

F

Q

S

R

L

A

A

E

R

A

S

S

K

R

G

A

V

G

K

L

I

H

G

F

F

E

Q

A

G

A

T

V

V

A

M

G

S

G

G

I

T

P

P

I

S

V

I

K

N

T

L

L

Y

R

R

E

-

S

V

L

L

A

P

G

G

N

S

T

R

I

V

E

I

R

K

V

I

S

R

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

A

-

E

N

E

K

K

G

L

V

S

S

F

F

H

E

V

E

C

A

L

L

G

K

E

E

A

A

Q

Q

R

R

L

R

G
|
G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

I

I

D

N

G

G

F

F

D

D

T

A

A

A

H

A

K

K

L

I

A

T

I

I

L

L

T

A

S

N

L

F

A

M

F

I

G

G

T

N

Q

S

V

V

D

T

A

I

D

K

S

D

I

V

Y

K

V

F

E

E

G

G

I

I

R

N

Q

R

L

-

T

-

L

-

A

-

D

D

L

L

E

P

A

K

A

N

D

E

D

-

L

-

G

-

Y

-

R

K

V

I

K

K

L

L

L

I

G

A

V

Y

A

A

V

D

R

E

T

K

E

E

T

V

G

W

I

-

E

-

Q

-

R

-

V

V

H

K

P

P

T

L

M

P

V

I

P

S

K

Q

H

D

W

D

P

P

I

L

A

Y

Q

N

V

V

S

D

G

G

V

V

T

E

N

N

A

A

V

L

A

E

V

I

D

T

G

T

D

D

A

I

V

Q

A

S

L

I

T

L

L

I

V

R

G

G

P

P

G

G

A

A

G
|
G

G

P

A

V

A

N

T

A

A

A

S

Y

A

G

V

A

V

L

G

S

D

D

L

L

F

I

D

L

V

L

A

K

R

R

Y8 (≠ L11) binding NADP(+)
N10 (≠ T13) binding NADP(+)
V11 (= V14) binding NADP(+)
R38 (≠ V39) binding NADP(+)
R39 (≠ T40) binding NADP(+)
S73 (≠ V77) binding NADP(+)
T100 (≠ A104) binding NADP(+)
K102 (= K106) binding NADP(+)
G182 (= G188) binding NADP(+)
E185 (= E191) binding NADP(+)
G284 (= G302) binding NADP(+)

Sites not aligning to the query:

303 modified: Interchain

7f4cA The crystal structure of the immature holo-enzyme of homoserine dehydrogenase complexed with NADP and 1,4-butandiol from the hyperthermophilic archaeon sulfurisphaera tokodaii. (see paper)
33% identity, 74% coverage: 4:319/428 of query aligns to 1:297/300 of 7f4cA

query
sites
7f4cA

L

M

K

K

V

L

G

L

L

L

A
x
F

G
|
G

L
x
Y

G
|
G

T
x
N

V
|
V

G

G

A

K

A

A

V

F

F

R

R

K

M

L

L

L

-

H

E

E

R

K

R

R

K

S

A

P

E

E

L

L

-

N

E

D

A

V

R

I

T

I

G

G

R

G

T

I

L

V

R
x
T

V
x
R

T
x
R

A

G

V

I

A

M

A

L

R

Q

D

D

R

K

S

-

R

E

D

D

R

F

G

T

L

P

D

D

L

L

G

E

G

G

V

D

I

V

W

F

-

K

-

A

F

F

E

E

D

-

P

-

V

-

A

K

L

I

A

K

R

P

D

D

G

I

D

I

I

V

D

D

V
|
V

F

-

V
x
S

E
x
S

L

A

M

N

G

Y

G

N

D

N

G

G

D

E

P

P

A

S

K

L

A

S

A

L

V

Y

A

K

A

E

A

A

L

I

D

K

R

D

G

G

I

V

P

N

V

I

T

T

A
x
T

N

N

K
|
K

A

A

L

P

L

L

A

A

K

L

C

A

G

F

V

N

E

E

L

I

A

F

Q

S

R

L

A

A

E

R

A

S

S

K

R

G

A

V

G

K

L

I

H

G

F

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

V

I

K

N

T

L

L

Y

R

R

E

-

S

V

L

L

A

P

G

G

N

S

T

R

I

V

E

I

R

K

V

I

S

R

G

G

I

I

L

L

N

N

G

G

T

T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

A

-

E

N

E

K

K

G

L

V

S

S

F

F

H

E

V

E

C

A

L

L

G

K

E

E

A

A

Q

Q

R

R

L

R

G

G

Y

Y

A

A

E
|
E

A

E

D

D

P

P

T

T

F

L

D

D

I

I

D

N

G

G

F

F

D

D

T

A

A

A

H

A

K

K

L

I

A

T

I

I

L

L

T

A

S

N

L

F

A

M

F

I

G

G

T

N

Q

S

V

V

D

T

A

I

D

K

S

D

I

V

Y

K

V

F

E

E

G

G

I

I

R

N

Q

R

L

-

T

-

L

-

A

-

D

D

L

L

E

-

A

-

D

-

L

-

G

-

Y

-

R

K

V

I

K

K

L

L

L

I

G

A

V

Y

A

A

V

D

R

E

T

K

E

E

T

V

G

W

I

-

E

-

Q

-

R

-

V

V

H

K

P

P

T

L

M

P

V

I

P

S

K

Q

H

D

W

D

P

P

I

L

A

Y

Q

N

V

V

S

D

G

G

V

V

T

E

N

N

A

A

V

L

A

E

V

I

D

T

G

T

D

D

A

I

V

Q

A

S

L

I

T

L

L

I

V

R

G

G

P

P

G

G

A

A

G
|
G

G

P

A

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

G

S

D

D

L

L

F

I

D

L

V

L

A

K

R

R

binding nadp nicotinamide-adenine-dinucleotide phosphate: F6 (≠ A9), G7 (= G10), Y8 (≠ L11), G9 (= G12), N10 (≠ T13), V11 (= V14), T37 (≠ R38), R38 (≠ V39), R39 (≠ T40), V72 (= V75), S73 (≠ V77), S74 (≠ E78), T100 (≠ A104), K102 (= K106), G127 (≠ A131), S131 (≠ G135), E185 (= E191), G280 (= G302), A284 (≠ T306)

5x9dA Crystal structure of homoserine dehydrogenase in complex with l- cysteine and NAD (see paper)
33% identity, 74% coverage: 4:319/428 of query aligns to 1:299/302 of 5x9dA

query
sites
5x9dA

L

M

K

K

V

L

G

L

L

L

A
x
F

G
|
G

L
x
Y

G
|
G

T
x
N

V
|
V

G

G

A

K

A

A

V

F

F

R

R

K

M

L

L

L

-

H

E

E

R

K

R

R

K

S

A

P

E

E

L

L

-

N

E

D

A

V

R

I

T

I

G

G

R

G

T

I

L

V

R
x
T

V
x
R

T

R

A

G

V

I

A

M

A

L

R

Q

D

D

R

K

S

-

R

E

D

D

R

F

G

T

L

P

D

D

L

L

G

E

G

G

V

D

I

V

W

F

-

K

-

A

F

F

E

E

D

-

P

-

V

-

A

K

L

I

A

K

R

P

D

D

G

I

D

I

I

V

D

D

V
|
V

F

-

V
x
S

E
x
S

L

A

M

N

G

Y

G

N

D

N

G

G

D

E

P
|
P

A

S

K

L

A

S

A

L

V

Y

A

K

A

E

A

A

L

I

D

K

R

D

G

G

I

V

P

N

V

I

T

T

A
x
T

N
|
N

K
|
K

A

A

L

P

L

L

A

A

K

L

C

A

G

F

V

N

E

E

L

I

A

F

Q

S

R

L

A

A

E

R

A

S

S

K

R

G

A

V

G

K

L

I

H

G

F

F

E

Q

A
x
G

A

T

V

V

A

M

G
x
S

G

G

I

T

P

P

I

S

V

I

K

N

T

L

L

Y

R

R

E

-

S

V

L

L

A

P

G

G

N

S

T

R

I

V

E

I

R

K

V

I

S

R

G

G

I

I

L

L

N
|
N

G
|
G

T
|
T

C

T

N

N

Y

F

I

I

L

L

T

T

R

L

M

M

A

-

E

N

E

K

K

G

L

V

S

S

F

F

H

E

V

E

C

A

L

L

G

K

E

E

A

A

Q

Q

R

R

L

R

G

G

Y
|
Y

A
|
A

E
|
E

A

E

D

D

P

P

T

T

F

L

D
|
D

I

I

D

N

G

G

F

F

D
|
D

T

A

A

A

H

A

K
|
K

L

I

A

T

I

I

L

L

T

A

S

N

L

F

A

M

F

I

G

G

T

N

Q

S

V

V

D

T

A

I

D

K

S

D

I

V

Y

K

V

F

E

E

G

G

I

I

R

N

Q

R

L

-

T

-

L

-

A

-

D

-

L

-

E

-

A

-

D

D

D

P

L

K

G

N

Y

E

R

K

V

I

K

K

L

L

L

I

G

A

V

Y

A

A

V

D

R

E

T

K

E

E

T

V

G

W

I

-

E

-

Q

-

R

-

V

V

H

K

P

P

T

L

M

P

V

I

P

S

K

Q

H

D

W

-

P

P

I

L

A

Y

Q

N

V

V

S

D

G

G

V

V

T

E

N

N

A

A

V

L

A

E

V

I

D

T

G

T

D

D

A

I

V

Q

A

S

L

I

T

L

L

I

V

R

G

G

P

P

G

G

A
|
A

G
|
G

G

P

A

V

A

N

T
x
A

A

A

S

Y

A

G

V

A

V

L

G

S

D

D

L

L

F

I

D

L

V

L

A

K

R

R

active site: D196 (= D202), K200 (= K206)
binding (2R)-3-[[(4S)-3-aminocarbonyl-1-[(2R,3R,4S,5R)-5-[[[[(2R,3S,4R,5R)-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl]oxy-oxidanyl-phosphoryl]oxymethyl]-3,4-bis(oxidanyl)oxolan-2-yl]-4H-pyridin-4-yl]sulfanyl]-2-azanyl-propanoic acid: F6 (≠ A9), G7 (= G10), Y8 (≠ L11), G9 (= G12), N10 (≠ T13), V11 (= V14), T37 (≠ R38), R38 (≠ V39), V72 (= V75), S73 (≠ V77), S74 (≠ E78), P82 (= P86), T100 (≠ A104), N101 (= N105), K102 (= K106), G127 (≠ A131), S131 (≠ G135), N155 (= N160), G156 (= G161), T157 (= T162), Y183 (= Y189), A184 (= A190), E185 (= E191), D191 (= D197), D196 (= D202), K200 (= K206), A281 (= A301), G282 (= G302), A286 (≠ T306)

4xb1A Hyperthermophilic archaeal homoserine dehydrogenase in complex with NADPH (see paper)
30% identity, 73% coverage: 3:315/428 of query aligns to 2:309/319 of 4xb1A

query
sites
4xb1A

E

K

L

V

K

N

V

I

G

S

L

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

A

R

A

A

V

L

F

-

R

-

M

-

L

-

E

-

R

-

K

-

A

A

E

E

L

I

E

I

A

A

R

E

T

K

G

S

R

R

T

I

L

F

R

G

V

V

-

E

-

L

T

N

A

V

V

I

A

S

A

I

R

T

D
|
D

R
|
R

S

S

R

-

D

-

R

-

G

-

L

-

D

-

L

-

G

-

G

G

V

T

I

I

W

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

-

K

-

E

-

V

F
x
K

E

E

D

S

P

T

V

G

A

K

L

L

A

S

R

N

D

I

G

G

D

D

I

Y

D

E

V

V

F

Y

-

N

-

F

-

S

-

P

-

Q

-

E

-

L

V

V

E

E

L

E

M

V

G

K

G

P

D

N

-

I

-

L

-

V

-

D

-
x
V

-
x
S

G

W

D

D

P

E

A

A

K

H

A

E

A

M

V

Y

A

K

A

V

A

A

L

L

D

G

R

E

G

G

I

I

P

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

K

N

C

Y

G

Y

V

D

E

E

L

L

A

M

Q

N

R

L

A

A

E

K

A

E

S

N

R

N

A

A

G

G

L

I

H

F

F

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

V

I

K

G

T

V

L

L

R

R

E

E

S

N

L

L

A

L

G

G

N

E

T

N

I

I

E

K

R

R

V

I

S

D

G

A

I

V

L

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

A

S

E

E

E

G

K

K

L

-

S

T

F

L

H

D

V

D

C

A

L

I

G

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

I

I

D

D

G

G

F

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

I

I

L

L

T

H

S

W

L

V

A

S

F

Y

G

G

T

E

Q

P

V

P

D

E

A

E

D

E

S

E

I

R

Y

L

V

-

E

-

G

G

I

I

R

R

Q

E

L

V

T

R

L

D

A

A

D

-

L

-

E

-

A

-

D

-

L

-

G

-

Y

R

R

N

V

V

K

R

L

L

L

V

G

A

V

Q

A

V

V

S

R

K

T

G

E

K

T

I

G

S

I

V

E

K

Q

P

R

R

V

K

H

L

P

S

T

S

M

D

V

N

P

P

K

-

H

-

W

-

P

-

I

-

A

L

Q

L

V

V

S

E

G

G

V

V

T

Q

N

N

A

A

V

A

A

V

V

I

D

R

G

T

D

N

A

N

V

L

A

G

-

E

L

V

T

I

L

L

V

K

G

G

P

P

G

G

A
x
G

G

G

A

R

A

V

T
|
T

A

A

S

S

A

G

V

V

V

F

G

T

D

D

L

I

F

I

active site: D211 (= D202), K215 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A9), F10 (≠ L11), G11 (= G12), T12 (= T13), V13 (= V14), D39 (= D46), R40 (= R47), K57 (≠ F61), V91 (vs. gap), S92 (vs. gap), S93 (vs. gap), S114 (≠ A104), K116 (= K106), S141 (≠ A131), G295 (≠ A301), T300 (= T306)

O58802 Homoserine dehydrogenase; HDH; HSD; HseDH; EC 1.1.1.3 from Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3) (see paper)
30% identity, 73% coverage: 3:315/428 of query aligns to 2:309/319 of O58802

query
sites
O58802

E

K

L

V

K

N

V

I

G

S

L

I

A

F

G

G

L
x
F

G

G

T
|
T

V
|
V

G

G

A

R

A

A

V

L

F

-

R

-

M

-

L

-

E

-

R

-

K

-

A

A

E

E

L

I

E

I

A

A

R

E

T

K

G

S

R

R

T

I

L

F

R

G

V

V

-

E

-

L

T

N

A

V

V

I

A

S

A

I

R

T

D

D

R
|
R

S

S

R

-

D

-

R

-

G

-

L

-

D

-

L

-

G

-

G

G

V

T

I

I

W

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

-

K

-

E

-

V

F
x
K

E

E

D

S

P

T

V

G

A

K

L

L

A

S

R

N

D

I

G

G

D

D

I

Y

D

E

V

V

F

Y

-

N

-

F

-

S

-

P

-

Q

-

E

-

L

V

V

E

E

L

E

M

V

G

K

G

P

D

N

-

I

-

L

-

V

-

D

-

V

-
x
S

G

W

D

D

P

E

A

A

K

H

A

E

A

M

V

Y

A

K

A

V

A

A

L

L

D

G

R

E

G

G

I

I

P

S

V

V

T

T

A
x
S

N

N

K
|
K

A

P

L

P

L

I

A

A

K

N

C

Y

G

Y

V

D

E

E

L

L

A

M

Q

N

R

L

A

A

E

K

A

E

S

N

R

N

A

A

G

G

L

I

H

F

F

F

E
|
E

A

S

A

T

V
|
V

A

M

G
x
A

G

G

I
x
T

P

P

I

I

V

I

K

G

T

V

L

L

R

R

E

E

S

N

L

L

A

L

G

G

N

E

T

N

I

I

E

K

R

R

V

I

S

D

G

A

I

V

L

V

N

N

G

A

T

S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

A

S

E

E

E

G

K

K

L

-

S

T

F

L

H

D

V

D

C

A

L

I

G

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D

D

I

I

D

D

G

G

F

I

D

D

T

A

A

Y

H

Y

K

K

L

A

A

K

I

I

L

L

T

H

S

W

L

V

A

S

F

Y

G

G

T

E

Q

P

V

P

D

E

A

E

D

E

S

E

I

R

Y

L

V

-

E

-

G

G

I

I

R

R

Q

E

L

V

T

R

L

D

A

A

D

-

L

-

E

-

A

-

D

-

L

-

G

-

Y

R

R

N

V

V

K

R

L

L

L

V

G

A

V

Q

A

V

V

S

R

K

T

G

E

K

T

I

G

S

I

V

E

K

Q

P

R

R

V

K

H

L

P

S

T

S

M

D

V

N

P

P

K

-

H

-

W

-

P

-

I

-

A

L

Q

L

V

V

S

E

G

G

V

V

T

Q

N

N

A

A

V

A

A

V

V

I

D

R

G

T

D

N

A

N

V

L

A

G

-

E

L

V

T

I

L

L

V

K

G

G

P

P

G

G

A

G

G
|
G

G

G

A

R

A

V

T

T

A

A

S

S

A

G

V

V

V

F

G

T

D

D

L

I

F

I

F10 (≠ L11) binding NADPH
T12 (= T13) binding NADPH
V13 (= V14) binding NADPH
R40 (= R47) binding NADPH; mutation to A: Increases activity with NADP.
K57 (≠ F61) binding NADPH; mutation to A: Increases activity with NADP.
S92 (vs. gap) binding NADPH
S93 (vs. gap) binding NADPH
S114 (≠ A104) binding NADPH
K116 (= K106) binding NADPH
E140 (= E130) binding Na(+)
V143 (= V133) binding Na(+)
A145 (≠ G135) binding Na(+)
T147 (≠ I137) binding Na(+)
G296 (= G302) binding NADPH

4xb2A Hyperthermophilic archaeal homoserine dehydrogenase mutant in complex with NADPH (see paper)
30% identity, 73% coverage: 3:315/428 of query aligns to 2:309/319 of 4xb2A

query
sites
4xb2A

E

K

L

V

K

N

V

I

G

S

L

I

A
x
F

G

G

L
x
F

G
|
G

T
|
T

V
|
V

G

G

A

R

A

A

V

L

F

-

R

-

M

-

L

-

E

-

R

-

K

-

A

A

E

E

L

I

E

I

A

A

R

E

T

K

G

S

R

R

T

I

L

F

R

G

V

V

-

E

-

L

T

N

A

V

V

I

A

S

A

I

R

T

D

D

R
|
R

S

S

R

-

D

-

R

-

G

-

L

-

D

-

L

-

G

-

G

G

V

T

I

I

W

W

-

G

-

D

-

F

-

D

-

L

-

E

-

A

-

K

-

E

-

V

F

A

E

E

D

S

P

T

V

G

A

K

L

L

A

S

R

N

D

I

G

G

D

D

I

Y

D

E

V

V

F

Y

-

N

-

F

-

S

-

P

-

Q

-

E

-

L

V

V

E

E

L

E

M

V

G

K

G

P

D

N

-

I

-

L

-

V

-

D

-
x
V

-
x
S

G

W

D

D

P

E

A

A

K

H

A

E

A

M

V

Y

A

K

A

V

A

A

L

L

D

G

R

E

G

G

I

I

P

S

V

V

T

T

A
x
S

N
|
N

K
|
K

A

P

L

P

L

I

A

A

K

N

C

Y

G

Y

V

D

E

E

L

L

A

M

Q

N

R

L

A

A

E

K

A

E

S

N

R

N

A

A

G

G

L

I

H

F

F

F

E

E

A
x
S

A

T

V

V

A

M

G

A

G

G

I

T

P

P

I

I

V

I

K

G

T

V

L

L

R

R

E

E

S

N

L

L

A

L

G

G

N

E

T

N

I

I

E

K

R

R

V

I

S

D

G

A

I

V

L

V

N

N

G
x
A

T
x
S

C

T

N

T

Y

F

I

I

L

L

T

T

R

K

M

M

A

S

E

E

E

G

K

K

L

-

S

T

F

L

H

D

V

D

C

A

L

I

G

E

E

E

A

A

Q

K

R

S

L

L

G

G

Y

I

A

L

E

E

A

E

D

D

P

P

T

S

F

K

D
|
D

I

I

D

D

G

G

F

I

D
|
D

T

A

A

Y

H

Y

K
|
K

L

A

A

K

I

I

L

L

T

H

S

W

L

V

A

S

F

Y

G

G

T

E

Q

P

V

P

D

E

A

E

D

E

S

E

I

R

Y

L

V

-

E

-

G

G

I

I

R

R

Q

E

L

V

T

R

L

D

A

A

D

-

L

-

E

-

A

-

D

-

L

-

G

-

Y

R

R

N

V

V

K

R

L

L

L

V

G

A

V

Q

A

V

V

S

R

K

T

G

E

K

T

I

G

S

I

V

E

K

Q

P

R

R

V

K

H

L

P

S

T

S

M

D

V

N

P

P

K

-

H

-

W

-

P

-

I

-

A

L

Q

L

V

V

S

E

G

G

V

V

T

Q

N

N

A

A

V

A

A

V

V

I

D

R

G

T

D

N

A

N

V

L

A

G

-

E

L

V

T

I

L

L

V

K

G

G

P

P

G

G

A
x
G

G

G

A

R

A

V

T
|
T

A

A

S

S

A

G

V

V

V

F

G

T

D

D

L

I

F

I

active site: D211 (= D202), K215 (= K206)
binding l-homoserine: A171 (≠ G161), S172 (≠ T162), D206 (= D197), K215 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: F8 (≠ A9), F10 (≠ L11), G11 (= G12), T12 (= T13), V13 (= V14), R40 (= R47), V91 (vs. gap), S92 (vs. gap), S93 (vs. gap), S114 (≠ A104), N115 (= N105), K116 (= K106), S141 (≠ A131), G295 (≠ A301), T300 (= T306)

3jsaA Homoserine dehydrogenase from thermoplasma volcanium complexed with NAD
30% identity, 54% coverage: 1:229/428 of query aligns to 1:239/321 of 3jsaA

query
sites
3jsaA

M

M

S

K

E

T

L

I

K

N

V

L

G

S

L

I

A

F

G
|
G

L
|
L

G
|
G

T
x
N

V
|
V

G

G

A

L

A

N

V

L

F

L

R

R

M

I

L

I

E

-

R

-

R

R

K

S

A

F

E

N

L

E

E

E

A

N

R

R

T

L

G

G

R

L

T

K

L

F

R

N

V

V

T

V

A

F

V

V

A

A

A
x
D

R
x
S

D

L

R

H

S

S

R

Y

-

Y

D

N

R

E

G

R

L

I

D

D

L

I

G

G

G

K

V

V

I

I

W

S

F

Y

E

K

D

E

-

K

-

G

-

S

-

L

-

D

-

S

-

L

-

E

-

Y

-

E

-

S

-

I

-

S

-

A

P

S

V

E

A

A

L

L

A

A

R

R

D

D

G

F

D

D

I

I

D

V

V

V

F

D

V
x
A

E
x
T

L
x
P

M

A

G

S

G

A

D

D

G

G

D

K

P

K

A

E

K

L

A

A

A

F

V

Y

A

K

A

E

A

T

L

F

D

E

R

N

G

G

I

K

P

D

V

V

T

T

A
|
A

N
|
N

K

K

A

S

L

G

L

L

A

A

K

N

C

F

G

W

V

P

E

E

L

I

A

M

Q

E

R

Y

A

A

E

R

A

S

S

N

R

N

A

R

G

R

L

I

H

R

F

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

I

L

V

F

K

S

T

F

L

I

R

D

E

Y

S

S

L

V

A

L

G

P

N

S

T

R

I

I

E

K

R

K

V

F

S

R

G

G

I

I

L

V

N

S

G

L

T

T

C

I

N

N

Y

Y

I

F

L

I

T

R

R

E

M

L

A

A

E

N

E

K

K

R

L

-

S

E

F

F

H

D

V

D

C

V

L

L

G

S

E

E

A

A

Q

T

R

K

L

L

G

G

Y

I

A

V

E

N

A

Y

D

K

P

D

T

-

F

-

D

D

I

L

D

T

G

G

F

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

A

V

I

I

L

L

T

C

S

N

L

H

A

L

F

Y

G

G

T

S

Q

S

V

Y

D

R

A

L

D

S

S

D

I

V

Y

F

V

Y

E

E

G

G

I

I

active site: D212 (= D202), K216 (= K206)
binding nicotinamide-adenine-dinucleotide: G10 (= G10), L11 (= L11), G12 (= G12), N13 (≠ T13), V14 (= V14), D42 (≠ A44), S43 (≠ R45), A90 (≠ V77), T91 (≠ E78), P92 (≠ L79), A117 (= A104), N118 (= N105), A144 (= A131)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 308

3ingA Crystal structure of homoserine dehydrogenase (np_394635.1) from thermoplasma acidophilum at 1.95 a resolution
26% identity, 73% coverage: 3:316/428 of query aligns to 2:300/319 of 3ingA

query
sites
3ingA

E

E

L

I

K

R

V

I

G

I

L

L

A

M

G
|
G

L
x
T

G
|
G

T
x
N

V
|
V

G

G

A

L

A

N

V

V

F

L

R

R

M

I

L

I

E

D

R

-

K

-

A

-

E

-

L

A

E

S

A

N

R

R

T

R

G

R

R

F

T

S

L

I

R

K

V

V

T

V

A

G

V

V

A

S

-
x
D

A
x
S

R

R

D

S

R

Y

S

A

R

S

D

G

R

R

G

N

L

L

D

D

L

I

G

S

V

I

I

I

W

S

F

N

E
x
K

D

E

P

K

V

T

A

G

L

R

A

I

R

S

D

D

G

R

D

A

I

F

D

S

V

G

F

P

V

E

E

D

L

L

M

M

G

G

G

E

D

A

G

A

D

D

-

L

-

V

-

D

-
x
C

-
x
T

P
|
P

A

A

K

S

-

R

-

D

-

G

-

V

-

R

-

E

-

Y

A

S

A

L

V

Y

A

R

A

M

A

A

L

F

D

E

R

S

G

G

I

M

P

N

V

V

T

T

A
|
A

N
|
N

K
|
K

A

S

L

G

L

L

A

A

K

N

C

K

G

W

V

H

E

D

L

I

A

M

Q

D

R

S

A

A

E

N

A

Q

S

N

R

S

A

K

G

Y

L

I

H

R

F

Y

E

E

A
|
A

A

T

V

V

A

A

G

G

I

V

P

P

I

L

V

F

K

S

T

V

L

L

R

D

E

Y

S

S

L

I

A

L

G

P

N

S

T

K

I

V

E

K

R

R

V

F

S

R

G

G

I

I

L

V

N

S

G

S

T

T

C

I

N

N

Y

Y

I

V

L

I

T

R

R

N

M

M

A

A

E

N

E

G

K

R

L

-

S

S

F

L

H

R

V

D

C

V

L

V

G

D

E

D

A

A

Q

I

R

K

L

K

G

G

Y

I

A

A

E
|
E

A
x
S

D

N

P

P

T

Q

F

D

D

D

I

L

D

N

G

G

F

L

D
|
D

T

A

A

A

H

R

K
|
K

L

S

A

V

I

I

L

L

T

V

S

N

L

H

A

I

F

F

G

G

T

T

Q

-

V

-

D

-

A

-

D

-

S

-

I

-

Y

-

V

-

E

-

G

-

I

-

R

-

Q

E

L

Y

T

T

L

L

A

N

D

D

L

V

E

E

A

Y

A

S

D

-

L

-

G

-

Y

-

R

-

V

-

K

-

L

-

G

G

V

V

A

D

V

E

R

R

T

S

E

Y

T

N

G

A

I

N

E

D

Q

R

R

L

V

V

H

T

P

E

T

V

M

Y

V

V

P

D

K

D

H

R

W

R

P

P

I

V

A

A

Q

-

V

-

S

-

G

-

V

V

T

S

N

R

A

I

V

I

A

S

V

L

D

N

G

K

D

D

A

D

V

F

A

L

L

M

T

S

L

I

V

G

G

M

P

D

G

G

A

L

G

G

G

Y

A

Q

-

I

-

E

-

T

-

D

A

S

T

N

A

G

S

T

A

V

V

N

V

V

G

S

D

D

L

I

F

Y

D

D

active site: D209 (= D202), K213 (= K206)
binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: G9 (= G10), T10 (≠ L11), G11 (= G12), N12 (≠ T13), V13 (= V14), D38 (vs. gap), S39 (≠ A44), K57 (≠ E62), C85 (vs. gap), T86 (vs. gap), P87 (= P86), A112 (= A104), N113 (= N105), K114 (= K106), A139 (= A131), E198 (= E191), S199 (≠ A192)

Sites not aligning to the query:

binding nadph dihydro-nicotinamide-adenine-dinucleotide phosphate: 302, 305

O81852 Bifunctional aspartokinase/homoserine dehydrogenase 2, chloroplastic; AK-HD 2; AK-HSDH 2; Beta-aspartyl phosphate homoserine 2; EC 2.7.2.4; EC 1.1.1.3 from Arabidopsis thaliana (Mouse-ear cress) (see paper)
28% identity, 74% coverage: 2:318/428 of query aligns to 556:909/916 of O81852

query
sites
O81852

S

T

E

T

L

L

K

A

V

M

G

G

L

I

A

V

G

G

L

P

G

G

T

L

V

I

G

G

A

A

A

T

V

L

F

L

R

D

M

Q

L

L

E

R

R

D

R

Q

K

A

A

A

E

V

L

L

E

K

A

Q

R

E

T

F

G

N

R

I

T

D

L

L

R

R

V

V

T

L

A

G

V

I

A

T

A

G

R

S

D

K

R

K

S

M

-

L

R

S

D

D

R

I

G

G

L

I

D

D

L

L

G

S

G

R

V

-

I

-

W

W

F

R

E

E

D

L

P

L

V

N

A

E

L

K

A

G

R

T

D

E

G

A

D

D

I

L

D

D

V

K

F

F

V

T

E

Q

L

Q

M

V

G

H

G

G

D

N

-

H

-

F

-

I

-

P

-

N

-

S

-

V

G

D

D

C

P

T

A

A

K

D

A

S

A

A

V

I

A

A

A

S

-

R

-

Y

-

D

A

W

L

L

D

R

R

K

G

G

I

I

P

H

V

V

V

I

T

T

A

P

N

N

K

K

A

K

L

-

A

A

K

N

C

S

G

G

-

P

-

L

-

D

-

Q

-

Y

V

L

E

K

L

L

A

R

Q

D

R

L

A

Q

E

R

A

K

S

S

R

Y

A

T

G

H

L

Y

H

F

F

Y

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

T

T

L

L

R

R

E

G

S

L

L

L

-

E

A

T

G

G

N

D

T

K

I

I

E

L

R

R

V

I

S

E

G

G

I

I

L

C

N

S

G

G

T

T

C

L

N

S

Y

Y

I

L

L

F

T

N

R

N

M

F

A

V

E

G

E

D

K

R

L

-

S

S

F

F

H

S

V

E

C

V

L

V

G

T

E

E

A

A

Q

K

R

N

L

A

G

G

Y

F

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

D

S

G

G

F

T

D

D

T

V

A

A

H

R

K

K

L

V

A

I

I

I

L

L

T

A

S

R

L

E

A

S

F

-

G

G

T

L

Q

K

V

L

D

D

-

L

A

A

D

D

-

L

-

P

-

I

-

R

S

S

I

L

Y

V

V

P

E

E

G

P

I

L

R

K

Q

G

L

C

T

T

L

S

A

V

D

E

-

E

-

F

L

M

E

E

A

K

-

L

-

P

-

Q

-

Y

-

D

-

G

-

D

-

L

-

A

-

K

-

E

-

R

-

L

-

D

A

A

D

E

D

N

L

S

G

G

Y

E

R

V

V

L

K

R

L

Y

L

V

G

G

V

V

-

V

-

D

A

A

V

V

R

N

T

Q

E

K

T

G

G

T

I

V

E

E

Q

L

R

R

V

R

H

Y

P

-

T

-

M

-

V

-

P

K

K

K

H

E

W

H

P

P

I

F

A

A

Q

Q

V

L

S

A

G

G

V

S

T

D

N

N

A

I

V

I

A

A

V

F

D

T

G

T

D

T

A

R

V

Y

-

K

-

D

-

H

A

P

L

L

T

I

L

V

V

R

G

G

P

P

G

G

A

A

G

G

G

A

A

Q

A

V

T

T

A

A

S

G

A

G

V

I

V

F

G

S

D

D

L

I

F

L

D

R

V

L

A

A

Sites not aligning to the query:

441 I→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
443 Q→A: Loss of threonine sensitivity for the aspartokinase activity and decreased inhibition of homoserine dehydrogenase activity by threonine.
522 I→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.
524 Q→A: No effect on the inhibition of aspartokinase activity by threonine, but decreased inhibition of homoserine dehydrogenase activity by threonine.

P31116 Homoserine dehydrogenase; HDH; HSD; EC 1.1.1.3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 3 papers)
29% identity, 35% coverage: 95:243/428 of query aligns to 106:260/359 of P31116

query
sites
P31116

L

V

D

E

R

N

G

G

I

I

P

S

V

I

V

A

T

T

A

P

N

N

K

K

-

K

A

A

L

F

L

S

A

S

K

D

C

L

G

A

V

T

E

W

L

K

A

A

Q

L

R

F

A

S

E

N

A

K

S

P

R

T

A

N

G

G

L

F

-

V

H

Y

F

H

E
|
E

A

A

A

T

V
|
V

A

G

G
x
A

G

G

I
x
L

P

P

I

I

V

I

K

S

T

F

L

L

R

R

E

E

S

I

L

I

-

Q

A

T

G

G

N

D

T

E

I

V

E

E

R

K

V

I

S

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

A

S

E

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

H

S

V

D

C

V

L

V

G

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E
|
E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

D

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

S

R

L

I

A

S

F

-

G

G

T

V

Q

E

V

V

D

E

A

S

D

P

S

T

I

S

Y

F

-

P

V

V

E

Q

G

S

I

L

R

I

Q

P

L

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

D

E

L

F

E143 (= E130) binding Na(+)
V146 (= V133) binding Na(+)
A148 (≠ G135) binding Na(+)
L150 (≠ I137) binding Na(+)
E208 (= E191) binding L-homoserine; mutation to D: Increases KM for aspartate-semialdehyde 48-fold and reduces kcat by 50%.; mutation E->L,Q: Loss of activity.
D219 (= D202) binding L-homoserine; mutation to L: Reduces kcat 150-fold.
K223 (= K206) mutation to V: Loss of activity.

Sites not aligning to the query:

13 binding NAD(+)
15 binding NAD(+)
16 binding NAD(+)
41 binding NAD(+)
79 H→A: Reduces kcat 2-fold.
93 binding NAD(+)
309 H→A: Reduces kcat 40-fold. Affects dimer formation.
340 binding NAD(+)

1tveA Homoserine dehydrogenase in complex with 4-(4-hydroxy-3- isopropylphenylthio)-2-isopropylphenol (see paper)
29% identity, 35% coverage: 95:243/428 of query aligns to 105:259/358 of 1tveA

query
sites
1tveA

L

V

D

E

R

N

G

G

I

I

P

S

V

I

V

A

T

T

A

P

N

N

K
|
K

-

K

A

A

L

F

L

S

A

S

K

D

C

L

G

A

V

T

E

W

L

K

A

A

Q

L

R

F

A

S

E

N

A

K

S

P

R

T

A

N

G

G

L

F

-

V

H

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

T

F

L

L

R

R

E

E

S

I

L

I

-

Q

A

T

G

G

N

D

T

E

I

V

E

E

R

K

V

I

S

E

G

G

I

I

L

F

N

S

G

G

T
|
T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

A

S

E

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

H

S

V

D

C

V

L

V

G

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D
|
D

I

L

D

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

S

R

L

I

A

S

F

-

G

G

T

V

Q

E

V

V

D

E

A

S

D

P

S

T

I

S

Y

F

-

P

V

V

E

Q

G

S

I

L

R

I

Q

P

L

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

D

E

L

F

active site: D218 (= D202), K222 (= K206)
binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: K116 (= K106), T175 (= T162), D213 (= D197), D218 (= D202)

Sites not aligning to the query:

binding 4-(4-hydroxy-3-isopropylphenylthio)-2-isopropylphenol: 93

1q7gA Homoserine dehydrogenase in complex with suicide inhibitor complex NAD-5-hydroxy-4-oxonorvaline (see paper)
29% identity, 35% coverage: 95:243/428 of query aligns to 105:259/358 of 1q7gA

query
sites
1q7gA

L

V

D

E

R

N

G

G

I

I

P

S

V

I

V

A

T

T

A
x
P

N

N

K
|
K

-

K

A

A

L

F

L

S

A

S

K

D

C

L

G

A

V

T

E

W

L

K

A

A

Q

L

R

F

A

S

E

N

A

K

S

P

R

T

A

N

G

G

L

F

-

V

H

Y

F

H

E

E

A
|
A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

T

F

L

L

R

R

E

E

S

I

L

I

-

Q

A

T

G

G

N

D

T

E

I

V

E

E

R

K

V

I

S

E

G

G

I

I

L

F

N
x
S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

A

S

E

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

H

S

V

D

C

V

L

V

G

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

D

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

S

R

L

I

A

S

F

-

G

G

T

V

Q

E

V

V

D

E

A

S

D

P

S

T

I

S

Y

F

-

P

V

V

E

Q

G

S

I

L

R

I

Q

P

L

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

D

E

L

F

active site: D218 (= D202), K222 (= K206)
binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: P114 (≠ A104), K116 (= K106), A143 (= A131), S173 (≠ N160), K222 (= K206)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide-5-hydroxy-4-oxonorvaline: 13, 14, 15, 39, 91, 92, 93, 97, 338, 343

1ebuD Homoserine dehydrogenase complex with NAD analogue and l-homoserine (see paper)
29% identity, 35% coverage: 95:243/428 of query aligns to 105:259/358 of 1ebuD

query
sites
1ebuD

L

V

D

E

R

N

G

G

I

I

P

S

V

I

V

A

T

T

A

P

N

N

K
|
K

-

K

A

A

L

F

L

S

A

S

K

D

C

L

G

A

V

T

E

W

L

K

A

A

Q

L

R

F

A

S

E

N

A

K

S

P

R

T

A

N

G

G

L

F

-

V

H

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

T

F

L

L

R

R

E

E

S

I

L

I

-

Q

A

T

G

G

N

D

T

E

I

V

E

E

R

K

V

I

S

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

A

S

E

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

H

S

V

D

C

V

L

V

G

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

D

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

S

R

L

I

A

S

F

-

G

G

T

V

Q

E

V

V

D

E

A

S

D

P

S

T

I

S

Y

F

-

P

V

V

E

Q

G

S

I

L

R

I

Q

P

L

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

D

E

L

F

active site: D218 (= D202), K222 (= K206)
binding 3-aminomethyl-pyridinium-adenine-dinucleotide: K116 (= K106)

Sites not aligning to the query:

binding 3-aminomethyl-pyridinium-adenine-dinucleotide: 11, 12, 13, 14, 15, 39, 40, 91, 93, 343

1ebfA Homoserine dehydrogenase from s. Cerevisiae complex with NAD+ (see paper)
29% identity, 35% coverage: 95:243/428 of query aligns to 105:259/358 of 1ebfA

query
sites
1ebfA

L

V

D

E

R

N

G

G

I

I

P

S

V

I

V

A

T

T

A
x
P

N

N

K

K

-

K

A

A

L

F

L

S

A

S

K

D

C

L

G

A

V

T

E

W

L

K

A

A

Q

L

R

F

A

S

E

N

A

K

S

P

R

T

A

N

G

G

L

F

-

V

H

Y

F

H

E

E

A

A

A

T

V

V

A

G

G

A

G

G

I

L

P

P

I

I

V

I

K

S

T

F

L

L

R

R

E

E

S

I

L

I

-

Q

A

T

G

G

N

D

T

E

I

V

E

E

R

K

V

I

S

E

G

G

I

I

L

F

N

S

G

G

T

T

C

L

N

S

Y

Y

I

I

L

F

T

N

R

E

M

F

A

S

E

T

E

S

K

Q

-

A

-

N

-

D

L

V

S

K

F

F

H

S

V

D

C

V

L

V

G

K

E

V

A

A

Q

K

R

K

L

L

G

G

Y

Y

A

T

E

E

A

P

D

D

P

P

T

R

F

D

D

D

I

L

D

N

G

G

F

L

D
|
D

T

V

A

A

H

R

K
|
K

L

V

A

T

I

I

L

V

T

G

S

R

L

I

A

S

F

-

G

G

T

V

Q

E

V

V

D

E

A

S

D

P

S

T

I

S

Y

F

-

P

V

V

E

Q

G

S

I

L

R

I

Q

P

L

K

T

P

L

L

A

E

D

S

L

V

E

K

A

S

A

A

D

D

D

E

L

F

active site: D218 (= D202), K222 (= K206)
binding nicotinamide-adenine-dinucleotide: P114 (≠ A104)

Sites not aligning to the query:

binding nicotinamide-adenine-dinucleotide: 10, 12, 13, 14, 15, 39, 40, 92, 93

Query Sequence

>WP_012115496.1 NCBI__GCF_000017645.1:WP_012115496.1
MSELKVGLAGLGTVGAAVFRMLERRKAELEARTGRTLRVTAVAARDRSRDRGLDLGGVIW
FEDPVALARDGDIDVFVELMGGDGDPAKAAVAAALDRGIPVVTANKALLAKCGVELAQRA
EASRAGLHFEAAVAGGIPIVKTLRESLAGNTIERVSGILNGTCNYILTRMAEEKLSFHVC
LGEAQRLGYAEADPTFDIDGFDTAHKLAILTSLAFGTQVDADSIYVEGIRQLTLADLEAA
DDLGYRVKLLGVAVRTETGIEQRVHPTMVPKHWPIAQVSGVTNAVAVDGDAVALTLVGPG
AGGAATASAVVGDLFDVARGARGYTFGLPVAELTKAERAAMQRHEGGYYIRLAVVNKPGT
AATIARRMADEQISLESIMQRRPDGAETARVILITYATTEEAVRRAIAAIEADGVISALP
QVIRIEKD

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory