SitesBLAST

SitesBLAST – Find functional sites

SitesBLAST

Comparing WP_012168766.1 NCBI__GCF_000010525.1:WP_012168766.1 to proteins with known functional sites using BLASTp with E ≤ 0.001.

Or try Sites on a Tree, PaperBLAST, Conserved Domains, or compare to all protein structures

Found 20 (the maximum) hits to proteins with known functional sites (download)

Q9VLJ8 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Ubiquitin activating enzyme 4; EC 2.7.7.80; EC 2.8.1.11 from Drosophila melanogaster (Fruit fly) (see paper)
48% identity, 89% coverage: 3:243/270 of query aligns to 64:297/453 of Q9VLJ8

query
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Q9VLJ8

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Sites not aligning to the query:

62 modified: Phosphothreonine

D4GSF3 SAMP-activating enzyme E1; Ubiquitin-like activating enzyme of archaea; Ubl-activating enzyme; EC 2.7.7.- from Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) (see paper)
47% identity, 100% coverage: 1:270/270 of query aligns to 3:266/270 of D4GSF3

query
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D4GSF3

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C188 (= C194) mutation to S: Loss of activity since this mutant is not able to complement a ubaA deletion in trans to restore sampylation and tRNA thiolation.

P38820 Adenylyltransferase and sulfurtransferase UBA4; Needs CLA4 to survive protein 3; Ubiquitin-like protein activator 4; EC 2.7.7.-; EC 2.8.1.- from Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) (see 4 papers)
47% identity, 81% coverage: 3:221/270 of query aligns to 39:252/440 of P38820

query
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P38820

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C225 (= C194) mutation C->A,S: Abolishes adenylyltransferase activity but not sulfurtransferase activity.

Sites not aligning to the query:

397 mutation C->A,S: Abolishes sulfurtransferase activity but not adenylyltransferase activity.

Q72J02 Sulfur carrier protein adenylyltransferase; E1-like protein TtuC; Sulfur carrier protein MoaD adenylyltransferase; Sulfur carrier protein ThiS adenylyltransferase; Sulfur carrier protein TtuB adenylyltransferase; tRNA two-thiouridine-synthesizing protein C; EC 2.7.7.80; EC 2.7.7.73; EC 2.7.7.- from Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) (see paper)
47% identity, 99% coverage: 1:267/270 of query aligns to 1:265/271 of Q72J02

query
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Q72J02

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C192 (= C194) modified: Glycyl cysteine thioester (Cys-Gly) (interchain with G-Cter in TtuB); mutation to S: Not able to form a thioester complex with TtuB.

Sites not aligning to the query:

268 C→S: Still able to form a thioester complex with TtuB.

O59954 Adenylyltransferase and sulfurtransferase uba4; Common component for nitrate reductase and xanthine dehydrogenase protein F; Ubiquitin-like protein activator 4; EC 2.7.7.80; EC 2.8.1.11 from Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans) (see paper)
45% identity, 88% coverage: 6:243/270 of query aligns to 64:306/482 of O59954

query
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O59954

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T

-

P

K

S

L

L

M

I

M

F

D

S

A

A

R

Y

S

S

M

S

-

P

R

Q

F

F

E

R

T

T

L

I

G82 (= G24) mutation to D: In cnxF21ts and cnxF24ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.
G100 (= G42) mutation to S: In cnxF1285; impairs molybdopterin biosynthesis.
R130 (= R72) mutation to Q: In cnxF200; impairs molybdopterin biosynthesis.
C185 (≠ G127) mutation to Y: In cnxF472; impairs molybdopterin biosynthesis.
E215 (≠ D157) mutation to K: In cnxF119; impairs molybdopterin biosynthesis.
G264 (= G205) mutation to S: In cnxF142ts; temperature-sensitive mutant. Impairs molybdopterin biosynthesis.

O95396 Adenylyltransferase and sulfurtransferase MOCS3; Molybdenum cofactor synthesis protein 3; Molybdopterin synthase sulfurylase; MPT synthase sulfurylase; EC 2.7.7.80; EC 2.8.1.11 from Homo sapiens (Human) (see 5 papers)
49% identity, 89% coverage: 2:240/270 of query aligns to 54:285/460 of O95396

query
sites
O95396

A

A

F

L

S

S

A

R

D

D

E

E

I

I

E

L

R

R

Y

Y

A

S

R

R

H

Q

I

L

V

V

L

L

H

P

D

E

V

L

G

G

G

V

P

H

G

G

Q

Q

Q

L

K

R

L

L

K

G

A

T

A

A

K

C

V

V

L

L

V

I

V

V

G

G

A

C

G

G

L

L

G

G

A

C

P

P

A

L

L

A

L

Q

Y

Y

L

L

A

A

G

G

V

V

G

G

R

R

L

L

I

G

I

L

V

V

D

D

D

Y

D

D

A

V

V

V

S

E

L

M

S

S

N

N

L

L

Q

A

R

R

Q

Q

V

V

I

L

H

H

R

G

T

E

A

A

D

L

I

A

G

G

R

Q

P

A

K

K

T

A

D

F

S

S

A

A

K

A

E

A

A

S

V

L

E

R

A

R

L

L

N

N

P

S

H

A

V

V

E

E

V

C

E

V

T

P

H

Y

A
x
T

V
x
Q

R
x
A

L
|
L

T
|
T

P
|
P

A
|
A

N
x
T

A
|
A

L
|
L

D
|
D

L
|
L

I
x
V

S
x
R

R
|
R

A
x
Y

D
|
D

V
|
V

L
x
A

D
|
D

G
x
C

S
|
S

D
|
D

N
|
N

F
x
V

A
x
P

T
|
T

R
|
R

Y
|
Y

L
|
L

V
|
V

S
x
N

D
|
D

A
|
A

C
|
C

V
|
V

L
|
L

A
|
A

K
x
G

K
x
R

T
x
P

L
|
L

V
|
V

T
x
S

A
|
A

A
x
S

L
x
A

G
x
L

T
x
R

F
|
F

D
x
E

A
x
G

T
x
Q

L
x
I

T
|
T

T
x
V

I
x
Y

S

-

A

-

H
|
H

E
x
Y

T
x
D

G
|
G

P

-

D

-

G

-

T

-

P

-

N
x
G

P
|
P

T
x
C

Y
|
Y

R
|
R

C
|
C

L
x
I

F
|
F

P
|
P

E
x
Q

P
|
P

P
x
A

G
x
E

T
|
T

V
|
V

A
x
T

P
x
N

C
|
C

A

A

E

D

A

G

G

G

V

V

L

L

G

G

A

V

L

V

A

T

G

G

I

V

A

L

G

G

S

C

M

L

A

Q

A

A

L

L

E

E

A

V

I

L

R

K

A

I

I

A

V

A

G

G

F

L

G

G

E

P

G

S

L

Y

V

S

G

G

K

S

L

L

M

L

M

F

D

D

A

A

R

L

S

R

M

G

R

H

F

F

158:238 (vs. 106:193, 51% identical) Interaction with NFS1
C239 (= C194) mutation to A: Impairs sulfurtransferase activity.

Sites not aligning to the query:

316 modified: Disulfide link with 324; C→A: Does not affect sulfurtransferase activity.
324 modified: Disulfide link with 316; C→A: Does not affect sulfurtransferase activity.
365 C→A: Does not affect sulfurtransferase activity.
412 active site, Cysteine persulfide intermediate; for sulfurtransferase activity; modified: Cysteine persulfide; C→A: Abolishes sulfurtransferase activity.
413 K→R: Does not affect sulfurtransferase specificity and activity.
414 L→K: Does not affect sulfurtransferase specificity and activity.
415 G→A: Does not affect sulfurtransferase specificity and activity.
416 N→V: Does not affect sulfurtransferase specificity and activity.
417 D→R: Results in 470-fold increased activity.; D→T: Results in 90-fold increased activity.
458 P→G: Does not affect sulfurtransferase specificity and activity.
460 Y→A: Does not affect sulfurtransferase specificity and activity.

P12282 Molybdopterin-synthase adenylyltransferase; MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase; Sulfur carrier protein MoaD adenylyltransferase; EC 2.7.7.80 from Escherichia coli (strain K12) (see 2 papers)
41% identity, 90% coverage: 3:244/270 of query aligns to 4:237/249 of P12282

query
sites
P12282

F

L

S

S

A

D

D

Q

E

E

I

M

E

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

H

R

D

G

V

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

V

I

V

V

G

G

A

L

G

G

L

L

G

G

A
x
C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

R

N

L

L

I

T

I

L

V

L

D

D

D

F

D

D

A

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

R

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K

K

T

V

D

E

S

S

A

A

K

R

E

D

A

A

V

L

E

T

A

R

L

I

N

N

P

P

H

H

V

I

E

A

V

I

E

-

T

-

H

-

A

-

V

-

R

-

L

-

T

T

P

P

A

V

N

N

A

A

L

L

-

L

-

D

-

A

-

E

-

L

-

A

D

A

L

L

I

I

S

A

R

E

A

H

D

D

V

L

V

V

L

L

D

D

G
x
C

S

T

D
|
D

N

N

F

V

A

A

T

V

R

R

Y

N

L

Q

V

L

S

N

D

A

A

G

C
|
C

V

F

L

A

A

A

K

K

K

V

T

P

L

L

V

V

T

S

A

G

A

A

L

A

G

I

T

R

F

M

D

E

A

G

T

Q

L

I

T

T

T

V

I

F

S

-

A

-

H

-

E

-

T

T

G

Y

P

Q

D

D

G

G

T

-

P

-

N

E

P

P

T
x
C

Y

Y

R

R

C
|
C

-

L

-

S

-

R

L

L

F

F

P

G

E

E

P

-

G

-

T

N

V

A

A

L

P

T

C
|
C

A

V

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

A

I

G

G

I

V

A

I

G

G

S

S

M

L

A

Q

A

A

L

M

E

E

A

A

I

I

R

K

A

M

I

L

V

A

G

G

F

Y

G

G

E

K

G

P

L

A

V

S

G

G

K

K

L

I

L

V

M

M

M

Y

D

D

A

A

R

M

S

T

M
x
C

R

Q

F

F

E

R

T

E

L

M

S

K

R14 (= R13) mutation R->A,K: No effect.; mutation to A: No activity; when associated with A-73.
C44 (≠ A43) mutation to A: No effect.
R73 (= R72) mutation to A: No effect. No activity; when associated with A-14.; mutation to K: Substantially reduced activity.
C128 (≠ G127) mutation to A: No effect.; mutation to Y: No activity.
D130 (= D129) mutation to A: No activity.; mutation to E: Substantially reduced activity.
C142 (= C141) mutation to A: No effect.
C172 (≠ T177) mutation to A: No zinc bound and no enzyme activity.
C175 (= C180) mutation to A: No zinc bound and no enzyme activity.
C187 (= C194) mutation to A: No effect.
C231 (≠ M238) mutation to A: No effect.

Sites not aligning to the query:

244 C→A: No zinc bound and almost no enzyme activity.
247 C→A: No zinc bound and almost no enzyme activity.

1jwbB Structure of the covalent acyl-adenylate form of the moeb-moad protein complex (see paper)
40% identity, 90% coverage: 3:244/270 of query aligns to 3:229/240 of 1jwbB

query
sites
1jwbB

F

L

S

S

A

D

D

Q

E

E

I

M

E

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

H

R

D

G

V

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

V

I

V

V

G
|
G

A

L

G
|
G

L

L

G

G

A

C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

R

N

L

L

I

T

I

L

V

L

D
|
D

D
x
F

D

D

A

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

R

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K
|
K

T

V

D

E

S

S

A

A

K

R

E

D

A

A

V

L

E

T

A

R

L

I

N

N

P

P

H

H

V

I

E

A

V

I

E

-

T

-

H

-

A

-

V

-

R

-

L

-

T

T

P

P

A

V

N

N

A

A

L
|
L

-

L

-

D

-

A

-

E

-

L

-

A

D

A

L

L

I

I

S

A

R

E

A

H

D

D

V

L

V

V

L

L

D

D

G
x
C

S
x
T

D
|
D

N
|
N

F

V

A

A

T
x
V

R

R

Y

N

L

Q

V

L

S

N

D

A

A

G

C

C

V

F

L

A

A

A

K

K

K

V

T

P

L

L

V

V

T

S

A

G

A

A

L

A

G

I

T

R

F

M

D

E

A

G

T

Q

L

I

T

T

T

V

I

F

S

-

A

-

H

-

E

-

T

T

G

Y

P

Q

D

D

G

G

T

-

P

-

N

E

P

P

T
x
C

Y

Y

R

R

C

C

L

L

F

-

P

-

E

-

P

-

G

-

T

-

V

S

A

R

P

L

C

F

A

G

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

A

I

G

G

I

V

A

I

G

G

S

S

M

L

A

Q

A

A

L

M

E

E

A

A

I

I

R

K

A

M

I

L

V

A

G

G

F

Y

G

G

E

K

G

P

L

A

V

S

G

G

K

K

L

I

L

V

M

M

M

Y

D

D

A

A

R

M

S

T

M

C

R

Q

F

F

E

R

T

E

L

M

S

K

active site: R13 (= R13), D129 (= D129)
binding adenosine monophosphate: G37 (= G37), G39 (= G39), G40 (= G40), D61 (= D61), F62 (≠ D62), K85 (= K85), L108 (= L115), C127 (≠ G127), T128 (≠ S128), D129 (= D129), N130 (= N130), V133 (≠ T133)
binding zinc ion: C171 (≠ T177)

Sites not aligning to the query:

binding zinc ion: 236, 239

1jw9B Structure of the native moeb-moad protein complex (see paper)
40% identity, 90% coverage: 3:244/270 of query aligns to 3:229/240 of 1jw9B

query
sites
1jw9B

F

L

S

S

A

D

D

Q

E

E

I

M

E

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

H

R

D

G

V

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

V

I

V

V

G

G

A

L

G

G

L

L

G

G

A

C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

R

N

L

L

I

T

I

L

V

L

D

D

D

F

D

D

A

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

T

I

L

H

H

R

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K

K

T

V

D

E

S

S

A

A

K

R

E

D

A

A

V

L

E

T

A

R

L

I

N

N

P

P

H

H

V

I

E

A

V

I

E

-

T

-

H

-

A

-

V

-

R

-

L

-

T

T

P

P

A

V

N

N

A

A

L

L

-

L

-

D

-

A

-

E

-

L

-

A

D

A

L

L

I

I

S

A

R

E

A

H

D

D

V

L

V

V

L

L

D

D

G

C

S

T

D
|
D

N

N

F

V

A

A

T

V

R

R

Y

N

L

Q

V

L

S

N

D

A

A

G

C

C

V

F

L

A

A

A

K

K

K

V

T

P

L

L

V

V

T

S

A

G

A

A

L

A

G

I

T

R

F

M

D

E

A

G

T

Q

L

I

T

T

T

V

I

F

S

-

A

-

H

-

E

-

T

T

G

Y

P

Q

D

D

G

G

T

-

P

-

N

E

P

P

T
x
C

Y

Y

R

R

C

C

L

L

F

-

P

-

E

-

P

-

G

-

T

-

V

S

A

R

P

L

C

F

A

G

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

A

I

G

G

I

V

A

I

G

G

S

S

M

L

A

Q

A

A

L

M

E

E

A

A

I

I

R

K

A

M

I

L

V

A

G

G

F

Y

G

G

E

K

G

P

L

A

V

S

G

G

K

K

L

I

L

V

M

M

M

Y

D

D

A

A

R

M

S

T

M

C

R

Q

F

F

E

R

T

E

L

M

S

K

active site: R13 (= R13), D129 (= D129)
binding zinc ion: C171 (≠ T177)

Sites not aligning to the query:

binding zinc ion: 236, 239

P30138 Sulfur carrier protein ThiS adenylyltransferase; EC 2.7.7.73 from Escherichia coli (strain K12) (see 3 papers)
42% identity, 88% coverage: 7:244/270 of query aligns to 5:233/251 of P30138

query
sites
P30138

E

D

I

F

E

M

R

R

Y

Y

A

S

R

R

H

Q

I

I

V

L

L

L

H

D

D

D

V

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

K

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

A

A

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

L

I

V

I

L

V

A

D

D

A

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

R

T

T

T

A

E

D

D

I

I

G

D

R

R

P

P

K

K

T

S

D

Q

S

V

A

S

K

Q

E

Q

A

R

V

L

E

T

A

Q

L

L

N

N

P

P

H

D

V

I

E

Q

V

L

E

T

T

A

H

L

A

Q

V

Q

R

R

L

L

T

T

P

G

A

E

N

A

A

L

L

K

D

D

L

A

I

V

S

A

R

R

A

A

D

D

V

V

L

L

D

D

G

C

S

T

D

D

N

N

F

M

A

A

T

T

R

R

Y

Q

L

E

V

I

S

N

D

A

A

A

C

C

V

V

L

A

A

L

K

N

K

T

T

P

L

L

V

I

T

T

A

A

A

S

L

A

G

V

T

G

F

F

D

G

A

G

T

Q

L

L

T

M

T

V

I

L

S

T

-

P

A

P

H

W

E

E

T

Q

G

G

P

-

D

-

G

-

T

-

P

-

N

-

P

-

T
x
C

Y

Y

R

R

C
|
C

L

L

F
x
W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

T

R

V

-

A

-

P

N

C
|
C

A

R

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

A

V

G

G

I

V

A

M

G

G

S

T

M

L

A

Q

A

A

L

L

E

E

A

A

I

I

R

K

A

L

I

L

V

S

G

G

F

I

G

-

E

E

G

T

L

P

V

A

G

G

K

E

L

L

L

R

M

L

M

F

D

D

A

G

R

K

S

S

M

S

R

Q

F

W

E

R

T

S

L

L

S

A

C169 (≠ T177) binding Zn(2+)
C172 (= C180) binding Zn(2+)
W174 (≠ F182) mutation to A: No adenylation of ThiS.
C184 (= C194) mutation to S: No cross-link formed with ThiS. No effect on ThiS thiocarboxylate formation in vitro. Does not support growth.

Sites not aligning to the query:

240 binding Zn(2+)
243 binding Zn(2+)

1zfnA Structural analysis of escherichia coli thif (see paper)
42% identity, 88% coverage: 7:244/270 of query aligns to 5:233/244 of 1zfnA

query
sites
1zfnA

E

D

I

F

E

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V

L

L

L

H

D

D

D

V

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

K

Q

V

V

L

L

V

I

V
x
I

G
|
G

A

L

G
|
G

L

L

G

G

A

T

P

P

A

A

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

L

I

V

I

L

V

A

D
|
D

D

D

A

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q
|
Q

V

I

I

L

H

F

R

T

T

T

A

E

D

D

I

I

G

D

R

R

P

P

K
|
K

T

S

D

Q

S

V

A

S

K

Q

E

Q

A

R

V

L

E

T

A

Q

L

L

N

N

P

P

H

D

V

I

E

Q

V

L

E

T

T

A

H

L

A

Q

V

Q

R

R

L

L

T

T

P

G

A

E

N

A

A

L

L

K

D

D

L

A

I

V

S

A

R

R

A

A

D

D

V

V

L

L

D

D

G

C

S
x
T

D
|
D

N

N

F

M

A

A

T
|
T

R

R

Y

Q

L

E

V

I

S

N

D

A

A

A

C

C

V

V

L

A

A

L

K

N

K

T

T

P

L

L

V

I

T

T

A

A

A

S

L

A

G

V

T

G

F

F

D

G

A

G

T

Q

L

L

T

M

T

V

I

L

S

T

-

P

A

P

H

W

E

E

T

Q

G

G

P

-

D

-

G

-

T

-

P

-

N

-

P

-

T
x
C

Y

Y

R

R

C
|
C

L

L

F

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

E

T

R

V

-

A

-

P

N

C

C

A

R

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

A

V

G

G

I

V

A

M

G

G

S

T

M

L

A

Q

A

A

L

L

E

E

A

A

I

I

R

K

A

L

I

L

V

S

G

G

F

I

G

-

E

E

G

T

L

P

V

A

G

G

K

E

L

L

L

R

M

L

M

F

D

D

A

G

R

K

S

S

M

S

R

Q

F

W

E

R

T

S

L

L

S

A

active site: R11 (= R13), D127 (= D129)
binding adenosine-5'-triphosphate: I34 (≠ V36), G35 (= G37), G37 (= G39), G38 (= G40), D59 (= D61), R70 (= R72), Q71 (= Q73), K83 (= K85), T126 (≠ S128), D127 (= D129), T131 (= T133)
binding zinc ion: C169 (≠ T177), C172 (= C180)

Sites not aligning to the query:

binding zinc ion: 240, 243

1zud3 Structure of this-thif protein complex (see paper)
41% identity, 88% coverage: 7:244/270 of query aligns to 5:228/240 of 1zud3

query
sites
1zud3

E

D

I

F

E

M

R

R

Y

Y

A

S

R
|
R

H

Q

I

I

V
x
L

L

L

H
x
D

D

D

V

I

G

A

G

L

P

D

G

G

Q

Q

K

K

L

L

K

L

A

D

A

S

K

Q

V

V

L

L

V

I

G

G

A

L

G

G

L

L

G

G

A

T

P

P

A

A

L

A

L

L

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

L

I

V

I

L

V

A

D

D

A

D

V

V

S

H

L

L

S

S

N

N

L

L

Q

Q

R

R

Q

Q

V

I

I

L

H

F

R

T

T

T

A

E

D

D

I

I

G

D

R

R

P

P

K

K

T

S

D

Q

S

V

A

S

K

Q

E

Q

A

R

V

L

E

T

A

Q

L

L

N

N

P

P

H

D

V

I

E

Q

V

L

E

T

T

A

H

L

A

Q

V

Q

R

R

L

L

T

T

P

G

A

E

N

A

A

L

L

K

D

D

L

A

I

V

S

A

R

R

A

A

D

D

V

V

L

L

D

D

G

C

S

T

D
|
D

N

N

F

M

A

A

T

T

R

R

Y

Q

L

E

V

I

S

N

D

A

A

A

C

C

V

V

L

A

A

L

K

N

K

T

T

P

L

L

V

I

T

T

A

A

A

S

L

A

G

V

T

G

F

F

D

G

A

G

T

Q

L

L

T

M

T

V

I

L

S

T

-

P

A

P

H

W

E

E

T

Q

G

G

P

-

D

-

G

-

T

-

P

-

N

-

P

-

T
x
C

Y

Y

R

R

C

C

L

L

F

W

P

P

E

D

P

N

P

Q

P

E

P

P

G

-

T

-

V

-

A

-

P

-

C

-

A

-

E

T

A

A

G

G

V

V

L

V

G

G

A

P

L

V

A

V

G

G

I

V

A

M

G

G

S

T

M

L

A

Q

A

A

L

L

E

E

A

A

I

I

R

K

A

L

I

L

V

S

G

G

F

I

G

-

E

E

G

T

L

P

V

A

G

G

K

E

L

L

L

R

M

L

M

F

D

D

A

G

R

K

S

S

M

S

R

Q

F

W

E

R

T

S

L

L

S

A

active site: R11 (= R13), D127 (= D129)
binding calcium ion: L14 (≠ V16), D16 (≠ H18)
binding zinc ion: C169 (≠ T177)

Sites not aligning to the query:

binding zinc ion: 235, 238

6yubA Crystal structure of uba4 from chaetomium thermophilum (see paper)
43% identity, 81% coverage: 2:220/270 of query aligns to 5:214/423 of 6yubA

query
sites
6yubA

A

S

F

L

S

S

A

K

D

E

E

E

I

I

E

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

H

P

D

G

V

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

K

R

L

L

K

M

A

N

A

A

K

K

V

V

L

L

V

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

A

A

L

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

I

I

G

I

I

V

V

D

D

D

G

D

D

A

S

V

V

S

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

R

A

T

T

A

K

D

R

I

V

G

G

R

M

P

L

K

K

T

V

D

D

S

S

A

L

K

I

E

T

A

H

V

L

E

I

A

E

L

I

N

N

P

P

H

L

V

P

E

V

V

Y

E

V

T

P

H

Y

A

R

V

F

R

D

L

L

T

T

P

P

A

Q

N

N

A

A

L

A

D

Q

L

I

I

I

S

K

R

P

A

W

D

D

V

V

V

I

L

L

D

D

G

C

S

T

D

D

N

N

F

P

A

A

T

T

R

R

Y

Y

L

L

V

I

S

S

D

D

A

V

C

C

V

V

L

L

A

L

K

G

K

K

T

P

L

L

V

V

T

S

A

A

L

S

G

V

T

Q

F

K

D

S

A

G

T

Q

L

L

T

I

T

V

I

L

S

N

A

C

H

P

E

P

T

T

G

-

P

P

D

Q

G

G

T

V

P

V

N

N

-

K

-

A

P

P

T
x
C

Y

Y

R

R

C
|
C

L

C

F

F

P

K

E

K

P

P

P

-

G

-

T

-

V

-

A

-

P

-

C

-

A

-

E

-

A

-

G

G

V

I

L

M

G

G

A

P

L

V

A

V

G

G

I

M

A

M

G

G

S

V

M

A

A

Q

A

A

L

G

E

E

A

A

I

I

R

K

A

I

I

L

V

V

binding zinc ion: C183 (≠ T177), C186 (= C180)

Sites not aligning to the query:

binding zinc ion: 266, 269

6yubB Crystal structure of uba4 from chaetomium thermophilum (see paper)
43% identity, 81% coverage: 2:220/270 of query aligns to 6:213/289 of 6yubB

query
sites
6yubB

A

S

F

L

S

S

A

K

D

E

E

E

I

I

E

E

R

R

Y

Y

A

S

R

R

H

Q

I

M

V

I

L

V

H

P

D

G

V

M

G

G

G

K

P

E

G

G

Q

Q

Q

L

K

R

L

L

K

M

A

N

A

A

K

K

V

V

L

L

V

I

G

G

A

A

G

G

L

L

G

G

A

C

P

P

A

A

L

A

L

Q

Y

Y

L

L

A

A

A

G

A

A

G

G

V

V

G

G

R

T

L

I

I

G

I

I

V

V

D

D

D

G

D

D

A

S

V

V

S

E

L

T

S

S

N

N

L

L

Q

H

R

R

Q

Q

V

V

I

A

H

H

R

A

T

T

A

K

D

R

I

V

G

G

R

M

P

L

K

K

T

V

D

D

S

S

A

L

K

I

E

T

A

H

V

L

E

I

A

E

L

I

N

N

P

P

H

L

V

P

E

V

V

Y

E

V

T

P

H

Y

A

R

V

F

R

D

L

L

T

T

P

P

A

Q

N

N

A

A

L

A

D

Q

L

I

I

I

S

K

R

P

A

W

D

D

V

V

V

I

L

L

D

D

G

C

S

T

D

D

N

N

F

P

A

A

T

T

R

R

Y

Y

L

L

V

I

S

S

D

D

A

V

C

C

V

V

L

L

A

L

K

G

K

K

T

P

L

L

V

V

T

S

A

A

L

S

G

V

T

Q

F

K

D

S

A

G

T

Q

L

L

T

I

T

V

I

L

S

N

A

C

H

P

E

P

T

T

G

-

P

P

D

Q

G

G

T

V

P

V

N

N

-

K

-

A

P

P

T
x
C

Y

Y

R

R

C
|
C

L

C

F

F

P

-

E

-

P

-

G

-

T

-

V

-

A

-

P

-

C

-

A

-

E

-

A

K

G

G

V

I

L

M

G

G

A

P

L

V

A

V

G

G

I

M

A

M

G

G

S

V

M

A

A

Q

A

A

L

G

E

E

A

A

I

I

R

K

A

I

I

L

V

V

binding zinc ion: C184 (≠ T177), C187 (= C180)

Sites not aligning to the query:

binding zinc ion: 265

1jwaB Structure of the atp-bound moeb-moad protein complex (see paper)
38% identity, 90% coverage: 3:244/270 of query aligns to 3:214/217 of 1jwaB

query
sites
1jwaB

F

L

S

S

A

D

D

Q

E

E

I

M

E

L

R

R

Y

Y

A

N

R
|
R

H

Q

I

I

V

I

L

L

H

R

D

G

V

F

G

D

G

F

P

D

G

G

Q

Q

Q

E

K

A

L

L

K

K

A

D

A

S

K

R

V

V

L

L

V

I

V

V

G

G

A

L

G
|
G

L

L

G

G

A

C

P

A

A

A

L

S

L

Q

Y

Y

L

L

A

A

A

S

A

A

G

G

V

V

G

G

R

N

L

L

I

T

I

L

V

L

D
|
D

D
x
F

D

D

A

T

V

V

S

S

L

L

S

S

N

N

L

L

Q

Q

R
|
R

Q

Q

V

T

I

L

H

H

R

S

T

D

A

A

D

T

I

V

G

G

R

Q

P

P

K
|
K

T

V

D

E

S

S

A

A

K

R

E

D

A

A

V

L

E

T

A

R

L

I

N

N

P

P

H

H

V

I

E

A

V

I

E

-

T

-

H

-

A

-

V

-

R

-

L

-

T

T

P

P

A

V

N

N

A

A

L
|
L

-

L

-

D

-

A

-

E

-

L

-

A

D

A

L

L

I

I

S

A

R

E

A

H

D

D

V

L

V

V

L

L

D

D

G

C

S

T

D
|
D

N
|
N

F

V

A

A

T
x
V

R

R

Y

N

L

Q

V

L

S

N

D

A

A

G

C

C

V

F

L

A

A

A

K

K

K

V

T

P

L

L

V

V

T

S

A

G

A

A

L

A

G

I

T

R

F

M

D

E

A

G

T

Q

L

I

T

T

T

V

I

F

S

-

A

-

H

-

E

-

T

-

G

-

P

-

D

-

G

-

T

-

P

-

N

-

P

-

T

T

Y

Y

R

-

C

-

L

-

F

-

P

-

E

-

P

-

G

-

T

-

V

-

A

-

P

-

C

-

A

-

E

E

A

A

G

G

V

V

L

M

G

A

A

P

L

L

A

I

G

G

I

V

A

I

G

G

S

S

M

L

A

Q

A

A

L

M

E

E

A

A

I

I

R

K

A

M

I

L

V

A

G

G

F

Y

G

G

E

K

G

P

L

A

V

S

G

G

K

K

L

I

L

V

M

M

M

Y

D

D

A

A

R

M

S

T

M

C

R

Q

F

F

E

R

T

E

L

M

S

K

active site: R13 (= R13), D129 (= D129)
binding adenosine-5'-triphosphate: G39 (= G39), G40 (= G40), D61 (= D61), F62 (≠ D62), R72 (= R72), K85 (= K85), L108 (= L115), D129 (= D129), N130 (= N130), V133 (≠ T133)

3h9jA Crystal structure of e. Coli mccb + ampcpp + semet mcca (see paper)
27% identity, 83% coverage: 4:226/270 of query aligns to 85:308/339 of 3h9jA

query
sites
3h9jA

S

N

A

S

D

T

E

E

I

N

E

N

R

R

Y

Y

A

S

R

R

H

N

I

F

V

L

L

H

H

Y

D

Q

V

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

Q

D

K

K

L

L

K

K

A

N

A

A

K

K

V

V

L

V

V

I

V

L

G

G

A

C

G
|
G

L

I

G

G

A

N

P

H

A

V

L

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

R

E

L

I

I

I

I

L

V

I

D
|
D

D

N

D
|
D

A

Q

V

I

S

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

I

L

H

F

R

S

T

E

A

D

D

D

I

V

G

G

R

K

P

N

K
|
K

T

T

D

E

S

V

A

I

K

K

E

R

A

E

V

L

E

L

A

K

L

R

N

N

P

S

H

E

V

I

E

S

V

V

E

S

T

E

H

I

A

A

V

L

R
x
N

L
x
I

T

N

P

D

A

Y

N

T

A

D

L

L

D

H

L

K

I

V

S

P

R

E

A

A

D

D

V

I

V

W

L

V

D

V

G

S

S
x
A

D
|
D

N
x
H

-

P

F

F

A

N

T

L

R

I

Y

N

L

W

V

V

S

N

D

K

A

Y

C

C

V

V

L

R

A

A

K

N

K

Q

T

P

L

Y

V

I

T

N

A

A

A

G

L

Y

G

V

T

N

F

D

D

I

A

A

T

V

L

F

T

-

I

-

S

-

A

-

H

-

E

-

T

-

G

G

P

P

D

L

G

Y

T

V

P

P

N

G

P

K

T

T

-

G

-
x
C

Y

Y

R

E

C

C

-

Q

-

K

-

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

L

L

F

I

P

N

E

S

P

R

P

F

P

K

P

P

G

A

T

T

V

F

A

A

P

P

C

-

A

-

E

-

A

-

G

-

V

-

L

-

G

-

A

-

L

V

A

N

G

N

I

V

A

A

G

A

S

A

M

L

A

C

A

A

L

A

E

D

A

V

I

I

R

K

A

F

I

I

V

G

G

K

F

Y

G

S

E

E

G

P

L

L

active site: R157 (= R72)
binding diphosphomethylphosphonic acid adenosyl ester: G124 (= G39), G125 (= G40), D146 (= D61), D148 (= D63), R157 (= R72), K170 (= K85), N193 (≠ R108), I194 (≠ L109), A213 (≠ S128), D214 (= D129), H215 (≠ N130)
binding zinc ion: C257 (vs. gap)

Sites not aligning to the query:

binding zinc ion: 334, 337
binding : 313, 315

3h5nA Crystal structure of e. Coli mccb + atp (see paper)
27% identity, 83% coverage: 4:226/270 of query aligns to 85:308/338 of 3h5nA

query
sites
3h5nA

S

N

A

S

D

T

E

E

I

N

E

N

R

R

Y

Y

A

S

R

R

H

N

I

F

V

L

L

H

H

Y

D

Q

V

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

Q

D

K

K

L

L

K

K

A

N

A

A

K

K

V

V

L

V

V

I

V

L

G

G

A

C

G
|
G

G

G

L

I

G

G

A

N

P

H

A

V

L

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

R

E

L

I

I

I

I

L

V

I

D
|
D

D

N

D

D

A

Q

V

I

S

E

L

N

S

T

N
|
N

L

L

Q

T

R
|
R

Q
|
Q

V

V

I

L

H

F

R

S

T

E

A

D

D

D

I

V

G

G

R

K

P

N

K
|
K

T

T

D

E

S

V

A

I

K

K

E

R

A

E

V

L

E

L

A

K

L

R

N

N

P

S

H

E

V

I

E

S

V

V

E

S

T

E

H

I

A

A

V

L

R
x
N

L
x
I

T

N

P

D

A

Y

N

T

A

D

L

L

D

H

L

K

I

V

S

P

R

E

A

A

D

D

V

I

V

W

L

V

D

V

G

S

S
x
A

D
|
D

N
x
H

-

P

F

F

A

N

T

L

R

I

Y

N

L

W

V

V

S

N

D

K

A

Y

C

C

V

V

L

R

A

A

K

N

K

Q

T

P

L

Y

V

I

T

N

A

A

A

G

L

Y

G

V

T

N

F

D

D

I

A

A

T

V

L

F

T

-

I

-

S

-

A

-

H

-

E

-

T

-

G

G

P

P

D

L

G

Y

T

V

P

P

N

G

P

K

T

T

-

G

-
x
C

Y

Y

R

E

C
|
C

-

Q

-

K

-

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

L

L

F

I

P

N

E

S

P

R

P

F

P

K

P

P

G

A

T

T

V

F

A

A

P

P

C

-

A

-

E

-

A

-

G

-

V

-

L

-

G

-

A

-

L

V

A

N

G

N

I

V

A

A

G

A

S

A

M

L

A

C

A

A

L

A

E

D

A

V

I

I

R

K

A

F

I

I

V

G

G

K

F

Y

G

S

E

E

G

P

L

L

active site: R157 (= R72)
binding adenosine-5'-triphosphate: G124 (= G39), D146 (= D61), N154 (= N69), R157 (= R72), Q158 (= Q73), K170 (= K85), N193 (≠ R108), I194 (≠ L109), A213 (≠ S128), D214 (= D129), H215 (≠ N130)
binding zinc ion: C257 (vs. gap), C260 (= C180)

Sites not aligning to the query:

binding zinc ion: 334, 337

3h5rA Crystal structure of e. Coli mccb + succinimide (see paper)
26% identity, 83% coverage: 4:226/270 of query aligns to 85:309/340 of 3h5rA

query
sites
3h5rA

S

N

A

S

D

T

E

E

I

N

E

N

R

R

Y

Y

A

S

R

R

H

N

I

F

V

L

L

H

H

Y

D

Q

V

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

Q

D

K

K

L

L

K

K

A

N

A

A

K

K

V

V

L

V

V

I

V

L

G

G

A

C

G

G

L
x
I

G

G

A

N

P

H

A

V

L

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

R

E

L

I

I

I

I

L

V

I

D

D

D

N

D

D

A

Q

V

I

S

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

I

L

H

F

R

S

T

E

A

D

D

D

I

V

G

G

R

K

P

N

K

K

T

T

D

E

S

V

A

I

K

K

E

R

A

E

V

L

E

L

A

K

L

R

N

N

P

S

H

E

V

I

E

S

V

V

E

S

T

E

H

I

A

A

V

L

R

N

L

I

T

N

P

D

A

Y

N

T

A

D

L

L

D

H

L

K

I

V

S

P

R

E

A

A

D

D

V

I

V

W

L

V

D

V

G
x
S

S
x
A

D

D

N

H

-

P

F

F

A

N

T

L

R

I

Y

N

L

W

V

V

S

N

D

K

A

Y

C

C

V

V

L

R

A

A

K

N

K

Q

T

P

L

Y

V

I

T

N

A
|
A

A
x
G

L
x
Y

G

V

T

N

F

D

D

I

A

A

T
x
V

L

F

T

-

I

-

S

-

A

-

H

-

E

-

T

-

G

G

P

P

D

L

G

Y

T

V

P

P

N

G

P

K

T

T

-

G

-
x
C

Y

Y

R

E

C
|
C

-

Q

-

K

-

V

-

K

-

E

-

N

-

I

-

D

-

H

-

K

-

I

-

K

L

L

F

I

P

N

E

S

P

R

P

F

P

K

P
|
P

G

A

T

T

V

F

A

A

P

P

C

-

A

-

E

-

A

-

G

-

V

-

L

-

G

-

A

-

L

V

A

N

G

N

I

V

A

A

G

A

S

A

M

L

A

C

A

A

L

A

E

D

A

V

I

I

R

K

A

F

I

I

V

G

G

K

F

Y

G

S

E

E

G

P

L

L

active site: R157 (= R72)
binding zinc ion: C257 (vs. gap), C260 (= C180)
binding : I126 (≠ L41), S212 (≠ G127), A213 (≠ S128), A237 (= A151), G238 (≠ A152), Y239 (≠ L153), V245 (≠ T159), P280 (= P188)

Sites not aligning to the query:

binding zinc ion: 335, 338
binding : 10, 14, 26, 314, 316

P20973 Ubiquitin-activating enzyme E1 1; EC 6.2.1.45 from Triticum aestivum (Wheat) (see paper)
34% identity, 57% coverage: 10:162/270 of query aligns to 444:601/1051 of P20973

query
sites
P20973

R

R

Y

Y

A

D

R

A

H

Q

I

I

V

S

L

V

H

-

D

-

V

F

G

G

G

S

P

K

G

L

Q

Q

Q

N

K

K

L

L

K

E

A

E

A

A

K

K

V

I

L

F

V

M

V

V

G

G

A

S

G

G

G

A

L

L

G

G

A

C

P

E

A

F

L

L

L

K

Y

N

L

L

A

A

A

L

A

M

G

G

V

I

-

S

-

C

-

S

-

Q

-

N

G

G

R

N

L

L

I

T

I

L

V

T

D

D

A

V

V

I

S

E

L

K

S

S

N

N

L

L

Q

S

R

R

Q

Q

V

F

I

L

H

F

R

R

T

D

A

W

D

N

I

I

G

G

R

Q

P

P

K

K

T

S

D

T

S

V

A

A

K

A

E

T

A

A

V

A

E

M

A

V

L

I

N

N

P

P

H

K

V

L

E

H

V

V

E

E

T

A

H

L

A

Q

V

N

R

R

L

A

T

S

P

P

-

E

-

T

A

E

N

N

A

V

L

F

D

N

-

D

-

A

L

F

I

W

S

E

R

N

A

L

D

D

V

A

V

V

L

V

D

N

G

A

S

L

D

D

N

N

F

V

A

T

T

A

R

R

Y

M

L

Y

V

I

S

D

D

S

A

R

C

C

V

V

L

Y

A

F

K

Q

K

K

T

P

L

L

V

L

T

E

A

S

A

-

L

-

G

G

T

T

F

L

D

G

A

A

T

K

L

C

T

N

T

T

Sites not aligning to the query:

626 active site, Glycyl thioester intermediate; C→A: Abolishes enzyme activity.

3h5aC Crystal structure of e. Coli mccb (see paper)
30% identity, 55% coverage: 4:151/270 of query aligns to 85:237/358 of 3h5aC

query
sites
3h5aC

S

N

A

S

D

T

E

E

I

N

E

N

R

R

Y

Y

A

S

R

R

H

N

I

F

V

L

L

H

H

Y

D

Q

V

S

G

Y

G

G

P

A

G

N

-

P

-

V

-

L

-

V

Q

Q

Q

D

K

K

L

L

K

K

A

N

A

A

K

K

V

V

L

V

V

I

V

L

G

G

A

C

G

G

L

I

G

G

A

N

P

H

A

V

L

S

L

V

Y

I

L

L

A

A

A

T

A

S

G

G

V

I

G

G

R

E

L

I

I

I

I

L

V

I

D

D

D

N

D

D

A

Q

V

I

S

E

L

N

S

T

N

N

L

L

Q

T

R
|
R

Q

Q

V

V

I

L

H

F

R

S

T

E

A

D

D

D

I

V

G

G

R

K

P

N

K

K

T

T

D

E

S

V

A

I

K

K

E

R

A

E

V

L

E

L

A

K

L

R

N

N

P

S

H

E

V

I

E

S

V

V

E

S

T

E

H

I

A

A

V

L

R

N

L

I

T

N

P

D

A

Y

N

T

A

D

L

L

D

H

L

K

I

V

S

P

R

E

A

A

D

D

V

I

V

W

L

V

D

V

G

S

S

A

D

D

N

H

-

P

F

F

A

N

T

L

R

I

Y

N

L

W

V

V

S

N

D

K

A

Y

C

C

V

V

L

R

A

A

K

N

K

Q

T

P

L

Y

V

I

T

N

A

A

active site: R157 (= R72)

Sites not aligning to the query:

binding zinc ion: 257, 260, 343

Query Sequence

>WP_012168766.1 NCBI__GCF_000010525.1:WP_012168766.1
MAFSADEIERYARHIVLHDVGGPGQQKLKAAKVLVVGAGGLGAPALLYLAAAGVGRLIIV
DDDAVSLSNLQRQVIHRTADIGRPKTDSAKEAVEALNPHVEVETHAVRLTPANALDLISR
ADVVLDGSDNFATRYLVSDACVLAKKTLVTAALGTFDATLTTISAHETGPDGTPNPTYRC
LFPEPPPPGTVAPCAEAGVLGALAGIAGSMAALEAIRAIVGFGEGLVGKLLMMDARSMRF
ETLSYGWDEDNPLTGTGARITDLSAHEKAA

Or try a new SitesBLAST search

SitesBLAST's Database

SitesBLAST's database includes (1) SwissProt entries with experimentally-supported functional features; and (2) protein structures with bound ligands, from the BioLip database.

by Morgan Price, Arkin group
Lawrence Berkeley National Laboratory